DPYD cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPYD
Entrez Gene ID	281124
Full Name	dihydropyrimidine dehydrogenase
General protein information	Preferred Names dihydropyrimidine dehydrogenase Names dihydropyrimidine dehydrogenase [NADP(+)] DHPDHase DPD dihydrothymine dehydrogenase dihydrouracil dehydrogenase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174041.2	NP_776466.1	dihydropyrimidine dehydrogenase [NADP(+)]

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DPYD	XP_513583.3
Gallus gallus (chicken)	DPYD	XP_426639.3
Xenopus tropicalis (tropical clawed frog)	dpyd	XP_002933845.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001021	XP_003437170.1
Homo sapiens (human)	DPYD	NP_000101.2
Canis lupus familiaris (dog)	DPYD	XP_005621932.1
Mus musculus (house mouse)	Dpyd	NP_740748.1
Danio rerio (zebrafish)	dpydb	NP_998058.1
Drosophila melanogaster (fruit fly)	su(r)	NP_572538.1
Caenorhabditis elegans (roundworm)	dpyd-1	NP_508927.2
Macaca mulatta (Rhesus monkey)	DPYD	XP_001106007.1
Bos taurus (cattle)	DPYD	NP_776466.1
Rattus norvegicus (Norway rat)	Dpyd	NP_112289.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

cDNA cloning of bovine liver dihydropyrimidine dehydrogenase.
Albin N, Johnson MR, Diasio RB
DNA sequence : the journal of DNA sequencing and mapping6(4)243-50(1996)

Dihydropyrimidine dehydrogenase activity in human peripheral blood mononuclear cells and liver: population characteristics, newly identified deficient patients, and clinical implication in 5-fluorouracil chemotherapy.
Lu Z, Zhang R, Diasio RB
Cancer research53(22)5433-8(1993 Nov)

Comparison of dihydropyrimidine dehydrogenase from human, rat, pig and cow liver. Biochemical and immunological properties.
Lu ZH, Zhang R, Diasio RB
Biochemical pharmacology46(5)945-52(1993 Sep)

Inactivation of dihydropyrimidine dehydrogenase by 5-iodouracil.
Porter DJ, Chestnut WG, Taylor LC, Merrill BM, Spector T
The Journal of biological chemistry266(30)19988-94(1991 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DPYD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPYD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41317	NM_174041.2 Latest version!	Bos taurus dihydropyrimidine dehydrogenase (DPYD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPYD ( NM_174041.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776466.1 Bos taurus dihydropyrimidine dehydrogenase (DPYD), mRNA.

Price & Availability↑

CloneID	OBa41317
Clone ID Related Accession (Same CDS sequence)	NM_174041.2
Accession Version	NM_174041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Bos taurus(cattle)
Product	dihydropyrimidine dehydrogenase [NADP(+)]
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U20981.1. On or before Oct 4, 2005 this sequence version replaced XM_865928.1, NM_174041.1. ##Evidence-Data-START## Transcript exon combination :: U20981.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCTG TGCTAAGCAA GGACGTGGCG GATATCGAGA GTATCCTGGC TTTAAATCCT 
CGAACACAGT CTCGTGCAAC TCTGCGTTCC ACTTTGGCCA AGAAATTAGA CAAGAAACAT 
TGGAAAAGAA ATCCTGATAA GAACTGCTTT AATTGCGAGA AGCTGGAGAA TAATTTTGAT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAAT GAGATGCCTG 
AAATGTGCAG ATGCCCCCTG TCAGAAGAGC TGTCCAACAA ATCTAGATAT CAAATCGTTC 
ATCACAAGTA TCTCAAACAA GAACTATTAT GGAGCTGCTA AGATGATATT TTCTGACAAC 
CCACTTGGTC TGACCTGTGG AATGGTATGC CCCACCTCTG ATCTTTGTGT AGGTGGATGT 
AATTTGTATG CCACTGAAGA GGGACCAATT AATATTGGTG GATTGCAGCA ATATGCTACT 
GAGGTATTCA AAGCAATGAA TATCCCACAA ATCAGGAATC CTTCTCTGCC TCCCCCAGAA 
AAAATGCCTG AAGCCTATTC TGCAAAGATT GCTCTTTTGG GTGCTGGGCC TGCAAGTATA 
AGTTGTGCTT CGTTCTTGGC TCGATTAGGC TACAATGACA TCACTATATT TGAAAAACAA 
GAATACGTTG GTGGTATAAG TACTTCTGAA ATCCCTCAGT TCCGGCTGCC ATATGATGTA 
GTAAATTTTG AGATTGAGCT TATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCAGTGA ATGACATTAC TCTTAGTACT TTGAAAGAAG AAGGGTACAA AGCTGCTTTC 
ATTGGGATAG GTTTGCCAGA ACCCAAGAAG GATCACATCT TCCAAGGCCT GACACAGGAC 
CAGGGGTTTT ACACTTCCAA AGACTTTCTG CCTCTTGTAG CCAAAAGCAG TAAAGCAGGA 
ATGTGCGCCT GTCACTCTCC ATTGCTGTCG ATACGGGGAA CCGTGATTGT ACTCGGAGCT 
GGAGACACAG CTTTCGACTG TGCAACATCC GCTTTACGTT GTGGAGCCCG CCGAGTGTTC 
ATCGTCTTCA GAAAAGGCTT TGTTAATATA AGAGCTGTCC CTGAGGAGGT GGAGCTTGCT 
AGAGAAGAAA AATGTGAATT TTTGCCTTTC TTGTCTCCAC GGAAGGTTAT AGTAAAAGGT 
GGGAGAATTG TTGCCATGCA ATTTGTTCGG ACAGAGCAAG ATGAAACTGG AAAATGGAAT 
GAAGATGGAG ATCAGATAGC CTGTCTGAAA GCCGATGTGG TCATCAGTGC CTTTGGCTCA 
GTTCTGAGTG ATCCTAAAGT AAAAGAAGCC TTGAGCCCTA TAAAATTTAA CAGATGGGAT 
CTCCCAGAAG TAGATCCAGA AACTATGCAA ACCAGTGAGC CATGGGTGTT TGCAGGTGGT 
GATGTGGTTG GTATAGCCAA CACTACAGTG GAAGCCGTGA ATGATGGAAA GCAAGCCTCT 
TGGTACATTC ACAGATATAT ACAGTCACAA TATGGAGCTT CAGTTTCTGC TAAGCCCGAA 
CTCCCCCTGT TTTATACTCC CATTGATCTG GTGGACATTA GTGTGGAAAT GGCTGCATTG 
AAGTTTACAA ATCCTTTTGG TCTTGCCAGT GCAACTCCAA CTACCAGTTC GTCAATGATT 
CGAAGAGCTT TTGAAGCTGG ATGGGCCTTT GCTCTGACCA AAACTTTCTC TCTTGATAAG 
GATATAGTGA CAAATGTTTC ACCCAGAATC ATCCGGGGGA CCACCTCTGG CCCCATGTAT 
GGCCCTGGAC AAAGCTCTTT CCTGAATATT GAGCTCATCA GTGAAAAAAC GGCTGCATAT 
TGGTGTCAAA GTGTCACTGA ACTAAAAGCC GACTTTCCAG ACAATATTGT GATTGCTAGC 
ATCATGTGCA GTTACAACAG AAATGACTGG ATGGAACTCT CCAGAAAGGC TGAGGCTTCT 
GGAGCAGACG CCCTGGAGTT AAATTTATCG TGTCCGCATG GCATGGGAGA AAGAGGAATG 
GGTCTGGCTT GTGGACAGGA TCCAGAGCTG GTGCGGAACA TCTGTCGCTG GGTTAGGCAA 
GCTGTTCGGA TTCCTTTTTT TGCCAAGTTG ACCCCAAATG TCACTGATAT TGTAAGCATA 
GCCAGAGCTG CAAAGGAAGG TGGGGCAAAT GGTGTTACAG CTACCAACAC TGTCTCAGGT 
CTCATGGGAT TAAAAGCTGA TGGCACACCC TGGCCAGCAG TGGGCCGTGA GAAGCGGACT 
ACATACGGCG GAGTGTCCGG CACAGCCATC AGACCTATTG CTTTGAGAGC TGTGACCACC 
ATTGCTCGTG CTTTGCCTGA ATTTCCCATT TTGGCCACTG GTGGAATTGA CTCAGCTGAA 
AGTGGTCTTC AGTTTCTCCA CGGTGGTGCT TCGGTGCTCC AGGTATGCAG TGCTATTCAA 
AATCAGGATT TCACTATCAT CCAAGACTAC TGCACTGGCC TCAAAGCCTT GCTTTATCTG 
AAAAGCATTG AAGAACTACA GGACTGGGAT GGGCAGAGTC CAGCCACGAA GAGTCACCAG 
AAAGGGAAAC CAGTCCCTTG TATTGCTGAA CTTGTGGGAA AGAAACTGCC AAGCTTTGGA 
CCTTATCTTG AGAAGTGCAA GAAAATCATA GCAGAGGAAA AGTTGAGACT GAAAAAAGAA 
AATGTGACCG TTCTACCACT TGAAAGAAAC CATTTTATCC CAAAAAAACC TATTCCTTCT 
GTTAAGGATG TGATTGGAAA AGCTCTGCAG TACCTTGGAA CATATGGTGA ACTGAACAAC 
ACAGAGCAGG TTGTGGCTGT GATCGATGAA GAGATGTGTA TCAACTGTGG CAAATGCTAC 
ATGACCTGTA ATGACTCTGG CTACCAGGCT ATCCAGTTTG ATCCTGAAAC CCACCTGCCC 
ACCGTTACTG ACACTTGTAC AGGCTGTACC CTGTGTCTCT CCGTCTGCCC TATTATCGAC 
TGCATCAAAA TGGTTTCCAG GACAACACCT TATGAACCAA AGAGAGGCTT GCCCTTGGCT 
GTGAATCCTG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776466.1
CDS	75..3152
Translation	MAPVLSKDVADIESILALNPRTQSRATLRSTLAKKLDKKHWKRNPDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKSFITSISNKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGCNLYATEEGPINIGGLQQYATEVFKAMNIPQIRNPSLPPPEKMPEAYSAKIALLGAGPASISCASFLARLGYNDITIFEKQEYVGGISTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSVNDITLSTLKEEGYKAAFIGIGLPEPKKDHIFQGLTQDQGFYTSKDFLPLVAKSSKAGMCACHSPLLSIRGTVIVLGAGDTAFDCATSALRCGARRVFIVFRKGFVNIRAVPEEVELAREEKCEFLPFLSPRKVIVKGGRIVAMQFVRTEQDETGKWNEDGDQIACLKADVVISAFGSVLSDPKVKEALSPIKFNRWDLPEVDPETMQTSEPWVFAGGDVVGIANTTVEAVNDGKQASWYIHRYIQSQYGASVSAKPELPLFYTPIDLVDISVEMAALKFTNPFGLASATPTTSSSMIRRAFEAGWAFALTKTFSLDKDIVTNVSPRIIRGTTSGPMYGPGQSSFLNIELISEKTAAYWCQSVTELKADFPDNIVIASIMCSYNRNDWMELSRKAEASGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVRIPFFAKLTPNVTDIVSIARAAKEGGANGVTATNTVSGLMGLKADGTPWPAVGREKRTTYGGVSGTAIRPIALRAVTTIARALPEFPILATGGIDSAESGLQFLHGGASVLQVCSAIQNQDFTIIQDYCTGLKALLYLKSIEELQDWDGQSPATKSHQKGKPVPCIAELVGKKLPSFGPYLEKCKKIIAEEKLRLKKENVTVLPLERNHFIPKKPIPSVKDVIGKALQYLGTYGELNNTEQVVAVIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVTDTCTGCTLCLSVCPIIDCIKMVSRTTPYEPKRGLPLAVNPVS

Target ORF information:

RefSeq Version	NM_174041.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dihydropyrimidine dehydrogenase (DPYD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174041.2

ORF Insert Sequence:

ATGGCCCCTG TGCTAAGCAA GGACGTGGCG GATATCGAGA GTATCCTGGC TTTAAATCCT 
CGAACACAGT CTCGTGCAAC TCTGCGTTCC ACTTTGGCCA AGAAATTAGA CAAGAAACAT 
TGGAAAAGAA ATCCTGATAA GAACTGCTTT AATTGCGAGA AGCTGGAGAA TAATTTTGAT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAAT GAGATGCCTG 
AAATGTGCAG ATGCCCCCTG TCAGAAGAGC TGTCCAACAA ATCTAGATAT CAAATCGTTC 
ATCACAAGTA TCTCAAACAA GAACTATTAT GGAGCTGCTA AGATGATATT TTCTGACAAC 
CCACTTGGTC TGACCTGTGG AATGGTATGC CCCACCTCTG ATCTTTGTGT AGGTGGATGT 
AATTTGTATG CCACTGAAGA GGGACCAATT AATATTGGTG GATTGCAGCA ATATGCTACT 
GAGGTATTCA AAGCAATGAA TATCCCACAA ATCAGGAATC CTTCTCTGCC TCCCCCAGAA 
AAAATGCCTG AAGCCTATTC TGCAAAGATT GCTCTTTTGG GTGCTGGGCC TGCAAGTATA 
AGTTGTGCTT CGTTCTTGGC TCGATTAGGC TACAATGACA TCACTATATT TGAAAAACAA 
GAATACGTTG GTGGTATAAG TACTTCTGAA ATCCCTCAGT TCCGGCTGCC ATATGATGTA 
GTAAATTTTG AGATTGAGCT TATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCAGTGA ATGACATTAC TCTTAGTACT TTGAAAGAAG AAGGGTACAA AGCTGCTTTC 
ATTGGGATAG GTTTGCCAGA ACCCAAGAAG GATCACATCT TCCAAGGCCT GACACAGGAC 
CAGGGGTTTT ACACTTCCAA AGACTTTCTG CCTCTTGTAG CCAAAAGCAG TAAAGCAGGA 
ATGTGCGCCT GTCACTCTCC ATTGCTGTCG ATACGGGGAA CCGTGATTGT ACTCGGAGCT 
GGAGACACAG CTTTCGACTG TGCAACATCC GCTTTACGTT GTGGAGCCCG CCGAGTGTTC 
ATCGTCTTCA GAAAAGGCTT TGTTAATATA AGAGCTGTCC CTGAGGAGGT GGAGCTTGCT 
AGAGAAGAAA AATGTGAATT TTTGCCTTTC TTGTCTCCAC GGAAGGTTAT AGTAAAAGGT 
GGGAGAATTG TTGCCATGCA ATTTGTTCGG ACAGAGCAAG ATGAAACTGG AAAATGGAAT 
GAAGATGGAG ATCAGATAGC CTGTCTGAAA GCCGATGTGG TCATCAGTGC CTTTGGCTCA 
GTTCTGAGTG ATCCTAAAGT AAAAGAAGCC TTGAGCCCTA TAAAATTTAA CAGATGGGAT 
CTCCCAGAAG TAGATCCAGA AACTATGCAA ACCAGTGAGC CATGGGTGTT TGCAGGTGGT 
GATGTGGTTG GTATAGCCAA CACTACAGTG GAAGCCGTGA ATGATGGAAA GCAAGCCTCT 
TGGTACATTC ACAGATATAT ACAGTCACAA TATGGAGCTT CAGTTTCTGC TAAGCCCGAA 
CTCCCCCTGT TTTATACTCC CATTGATCTG GTGGACATTA GTGTGGAAAT GGCTGCATTG 
AAGTTTACAA ATCCTTTTGG TCTTGCCAGT GCAACTCCAA CTACCAGTTC GTCAATGATT 
CGAAGAGCTT TTGAAGCTGG ATGGGCCTTT GCTCTGACCA AAACTTTCTC TCTTGATAAG 
GATATAGTGA CAAATGTTTC ACCCAGAATC ATCCGGGGGA CCACCTCTGG CCCCATGTAT 
GGCCCTGGAC AAAGCTCTTT CCTGAATATT GAGCTCATCA GTGAAAAAAC GGCTGCATAT 
TGGTGTCAAA GTGTCACTGA ACTAAAAGCC GACTTTCCAG ACAATATTGT GATTGCTAGC 
ATCATGTGCA GTTACAACAG AAATGACTGG ATGGAACTCT CCAGAAAGGC TGAGGCTTCT 
GGAGCAGACG CCCTGGAGTT AAATTTATCG TGTCCGCATG GCATGGGAGA AAGAGGAATG 
GGTCTGGCTT GTGGACAGGA TCCAGAGCTG GTGCGGAACA TCTGTCGCTG GGTTAGGCAA 
GCTGTTCGGA TTCCTTTTTT TGCCAAGTTG ACCCCAAATG TCACTGATAT TGTAAGCATA 
GCCAGAGCTG CAAAGGAAGG TGGGGCAAAT GGTGTTACAG CTACCAACAC TGTCTCAGGT 
CTCATGGGAT TAAAAGCTGA TGGCACACCC TGGCCAGCAG TGGGCCGTGA GAAGCGGACT 
ACATACGGCG GAGTGTCCGG CACAGCCATC AGACCTATTG CTTTGAGAGC TGTGACCACC 
ATTGCTCGTG CTTTGCCTGA ATTTCCCATT TTGGCCACTG GTGGAATTGA CTCAGCTGAA 
AGTGGTCTTC AGTTTCTCCA CGGTGGTGCT TCGGTGCTCC AGGTATGCAG TGCTATTCAA 
AATCAGGATT TCACTATCAT CCAAGACTAC TGCACTGGCC TCAAAGCCTT GCTTTATCTG 
AAAAGCATTG AAGAACTACA GGACTGGGAT GGGCAGAGTC CAGCCACGAA GAGTCACCAG 
AAAGGGAAAC CAGTCCCTTG TATTGCTGAA CTTGTGGGAA AGAAACTGCC AAGCTTTGGA 
CCTTATCTTG AGAAGTGCAA GAAAATCATA GCAGAGGAAA AGTTGAGACT GAAAAAAGAA 
AATGTGACCG TTCTACCACT TGAAAGAAAC CATTTTATCC CAAAAAAACC TATTCCTTCT 
GTTAAGGATG TGATTGGAAA AGCTCTGCAG TACCTTGGAA CATATGGTGA ACTGAACAAC 
ACAGAGCAGG TTGTGGCTGT GATCGATGAA GAGATGTGTA TCAACTGTGG CAAATGCTAC 
ATGACCTGTA ATGACTCTGG CTACCAGGCT ATCCAGTTTG ATCCTGAAAC CCACCTGCCC 
ACCGTTACTG ACACTTGTAC AGGCTGTACC CTGTGTCTCT CCGTCTGCCC TATTATCGAC 
TGCATCAAAA TGGTTTCCAG GACAACACCT TATGAACCAA AGAGAGGCTT GCCCTTGGCT 
GTGAATCCTG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

cDNA cloning of bovine liver dihydropyrimidine dehydrogenase.
DNA sequence : the journal of DNA sequencing and mapping6(4)243-50(1996)
Albin N,Johnson MR,Diasio RB

Dihydropyrimidine dehydrogenase activity in human peripheral blood mononuclear cells and liver: population characteristics, newly identified deficient patients, and clinical implication in 5-fluorouracil chemotherapy.
Cancer research53(22)5433-8(1993 Nov)
Lu Z,Zhang R,Diasio RB

Comparison of dihydropyrimidine dehydrogenase from human, rat, pig and cow liver. Biochemical and immunological properties.
Biochemical pharmacology46(5)945-52(1993 Sep)
Lu ZH,Zhang R,Diasio RB

Inactivation of dihydropyrimidine dehydrogenase by 5-iodouracil.
The Journal of biological chemistry266(30)19988-94(1991 Oct)
Porter DJ,Chestnut WG,Taylor LC,Merrill BM,Spector T

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DPYD cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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