Ifih1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ifih1
Entrez Gene ID	71586
Full Name	interferon induced with helicase C domain 1
Synonyms	9130009C22Rik,Helicard,Hlcd,MDA5,RLR-2
General protein information	Preferred Names interferon induced with helicase C domain 1 Names interferon-induced helicase C domain-containing protein 1 RIG-I-like receptor 2 helicase with 2 CARD domains interferon induced with helicase C domain protein 1 melanoma differentiation-associated protein 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 C1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_027835.3	NP_082111.2	interferon-induced helicase C domain-containing protein 1 isoform 1
NM_001164477.1	NP_001157949.1	interferon-induced helicase C domain-containing protein 1 isoform 2
XM_006500187.4	XP_006500250.1	interferon-induced helicase C domain-containing protein 1 isoform X1
XM_006500187.3	XP_006500250.1	interferon-induced helicase C domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC565759	XP_694124.4
Bos taurus (cattle)	IFIH1	XP_002685384.1
Mus musculus (house mouse)	Ifih1	NP_082111.2
Rattus norvegicus (Norway rat)	Ifih1	NP_001102669.1
Homo sapiens (human)	IFIH1	NP_071451.2
Pan troglodytes (chimpanzee)	IFIH1	XP_003309348.1
Macaca mulatta (Rhesus monkey)	IFIH1	NP_001040588.1
Canis lupus familiaris (dog)	IFIH1	XP_545493.2
Gallus gallus (chicken)	IFIH1	NP_001180567.1
Xenopus tropicalis (tropical clawed frog)	ifih1	XP_002933320.2

Gene Ontology
Bibliography

Related articles in PubMed

Noncoding dsRNA induces retinoic acid synthesis to stimulate hair follicle regeneration via TLR3.
Kim D, Chen R, Sheu M, Kim N, Kim S, Islam N, Wier EM, Wang G, Li A, Park A, Son W, Evans B, Yu V, Prizmic VP, Oh E, Wang Z, Yu J, Huang W, Archer NK, Hu Z, Clemetson N, Nelson AM, Chien A, Okoye GA, Miller LS, Ghiaur G, Kang S, Jones JW, Kane MA, Garza LA
Nature communications10(1)2811(2019 06)

CCR5 is a required signaling receptor for macrophage expression of inflammatory genes in response to viral double-stranded RNA.
Shaheen ZR, Christmann BS, Stafford JD, Moran JM, Buller RML, Corbett JA
American journal of physiology. Regulatory, integrative and comparative physiology316(5)R525-R534(2019 05)

Distinct roles for MDA5 and TLR3 in the acute response to inhaled double-stranded RNA.
Veazey JM, Chapman TJ, Smyth TR, Hillman SE, Eliseeva SI, Georas SN
PloS one14(5)e0216056(2019)

Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis.
Yu Q, Qu K, Modis Y
Molecular cell72(6)999-1012.e6(2018 12)

ADAR1-mediated RNA editing is required for thymic self-tolerance and inhibition of autoimmunity.
Nakahama T, Kato Y, Kim JI, Vongpipatana T, Suzuki Y, Walkley CR, Kawahara Y
EMBO reports19(12)(2018 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ifih1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ifih1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21872	NM_001164477.1 Latest version!	Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu30898	XM_006500187.4 Latest version!	Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu21680	NM_027835.3 Latest version!	Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu30898	XM_006500187.3	Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ifih1 ( NM_001164477.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157949.1 Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu21872
Clone ID Related Accession (Same CDS sequence)	NM_001164477.1
Accession Version	NM_001164477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Mus musculus(house mouse)
Product	interferon-induced helicase C domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929246.7. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC080200.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGATTG TCTGTTCTGC AGAGGACAGC TTCAGGAATC TCATCTTATT CTTCAGGCCC 
AGGCTGAAAA TGTACATTCA GGTGGAGCCA GTGCTGGACC ACCTCATCTT TCTGTCTGCA 
GAAACCAAAG AGCAGATTCT TAAAAAGATC AACACCTGTG GTAACACCAG CGCGGCAGAA 
CTGCTGCTGA GCACCTTGGA GCAGGGACAA TGGCCTCTGG GATGGACGCA GATGTTCGTG 
GAGGCCCTAG AGCACAGTGG CAATCCCCTA GCCGCGCGCT ATGTCAAACC CACACTCACT 
GATCTGCCCT CTCCTTCCTC TGAGACTGCC CATGACGAGT GTCTCCACTT GCTGACCCTC 
CTCCAGCCCA CTTTGGTGGA CAAACTTCTG ATTAACGATG TCTTGGACAC TTGCTTCGAG 
AAGGGACTAT TGACAGTCGA AGACAGAAAT CGGATTTCTG CTGCAGGAAA CAGCGGGAAT 
GAGTCAGGTG TAAGAGAGCT GCTGAGAAGG ATTGTGCAGA AGGAAAACTG GTTTTCTACC 
TTCCTGGATG TTCTGCGCCA AACTGGAAAT GATGCACTAT TCCAAGAACT AACAGGTGGA 
GGCTGCCCAG AAGACAACAC AGAATCAGAC ACAAGTTTGG CAGAAGGAAG TGTCAGCTGC 
TTCGATGAAA GTCTTGGACA TAACAGCAAC ATGGGCAGGG ATTCAGGCAC CATGGGAAGT 
GATTCAGATG AAAGTGTCAT CCAGACAAAA AGAGTATCCC CCGAGCCAGA ACTGCAGCTC 
AGGCCTTACC AAATGGAAGT GGCCCAACCA GCTCTAGATG GGAAGAATAT TATTATCTGC 
CTCCCCACGG GGAGTGGGAA AACCAGAGTG GCTGTTTACA TCACCAAAGA TCACTTAGAC 
AAGAAGAAGC AGGCATCTGA ATCCGGGAAG GTTATCGTTC TTGTCAATAA GGTAATGTTA 
GCAGAACAAC TTTTCCGAAA AGAGTTCAAC CCATATTTGA AGAAATGGTA TCGAATTATT 
GGATTAAGTG GCGATACCCA GCTGAAAATA TCATTTCCAG AAGTTGTCAA ATCTTACGAT 
GTTATTATCA GCACTGCTCA AATCCTTGAA AACTCCCTCT TAAATCTGGA GAGTGGAGAC 
GATGACGGTG TGCAGCTGTC AGACTTCTCT CTCATTATCA TTGATGAGTG CCATCACACC 
AACAAGGAGG CAGTCTATAA CAACATCATG AGACGATATT TGAAGCAGAA GCTGAGAAAC 
AATGACCTCA AGAAACAAAA CAAACCAGCC ATTCCCCTGC CGCAGATACT AGGACTGACA 
GCTTCACCTG GTGTTGGAGC AGCCAAAAAG CAGTCTGAGG CTGAAAAACA TATTTTAAAT 
ATATGTGCCA ATCTTGATGC CTTTACCATT AAAACAGTGA AAGAGAATCT TGGTCAACTC 
AAACACCAAA TAAAGGAACC ATGCAAGAAA TTTGTGATTG CTGATGACAC CAGAGAAAAT 
CCATTTAAAG AGAAACTTCT AGAAATTATG GCAAGCATTC AGACTTACTG CCAAAAAAGT 
CCAATGTCAG ATTTTGGAAC CCAACATTAT GAGCAGTGGG CCATTCAAAT GGAGAAAAAA 
GCTGCTAAAG ACGGAAATCG CAAAGATCGC GTCTGTGCAG AACATTTGAG GAAGTACAAC 
GAAGCCCTAC AAATCAACGA CACGATCCGA ATGATTGATG CATATAGCCA CCTGGAGACA 
TTCTACACTG ATGAGAAAGA AAAGAAGTTC GCAGTCCTCA ATGACAGCGA CAAGAGTGAT 
GACGAGGCCA GCAGTTGCAA TGACCAACTT AAGGGCGATG TAAAGAAATC TTTGAAACTG 
GACGAAACGG ATGAATTTCT CATGAATTTG TTCTTTGATA ACAAGAAAAT GTTGAAAAAA 
CTAGCTGAAA ACCCAAAATA CGAGAATGAA AAACTCATTA AATTAAGAAA CACGATACTG 
GAACAATTCA CAAGGTCTGA GGAGTCCTCC CGAGGAATTA TTTTTACAAA AACACGACAG 
AGCACCTACG CACTTTCCCA GTGGATCATG GAAAATGCAA AGTTTGCGGA AGTTGGAGTC 
AAAGCGCATC ACCTGATTGG CGCGGGGCAC AGCAGTGAAG TCAAGCCCAT GACTCAGACT 
GAACAAAAAG AAGTCATTAG TAAATTTCGC ACTGGCGAAA TAAATCTGCT TATCGCTACG 
ACGGTGGCAG AGGAAGGCCT GGATATCAAA GAGTGCAATA TTGTTATTCG TTATGGCCTT 
GTCACGAACG AGATAGCCAT GGTCCAGGCC CGGGGTCGAG CCAGAGCTGA TGAAAGCACG 
TATGTCCTGG TCACCAGCAG TGGCTCAGGA GTTACCGAAC GGGAGATTGT TAATGATTTC 
CGAGAGAAGA TGATGTATAA AGCTATTAAC CGTGTTCAAA ACATGAAACC AGAGGAGTAT 
GCACATAAGA TTTTGGAATT GCAGGTGCAA AGTATCCTGG AAAAGAAAAT GAAAGTCAAA 
AGAAGCATTG CAAAGCAATA CAACGACAAT CCATCGTTAA TAACACTTCT CTGCAAAAAT 
TGTAGCATGC TGGTCTGCTC GGGAGAAAAC ATCCATGTCA TTGAGAAGAT GCATCATGTC 
AATATGACAC CAGAATTCAA GGGACTCTAC ATTGTAAGAG AAAACAAAGC ACTGCAAAAG 
AAATTTGCTG ATTATCAGAC CAATGGAGAG ATTATCTGCA AGTGTGGCCA GGCTTGGGGA 
ACAATGATGG TGCACAAAGG TTTAGATTTG CCTTGTCTTA AAATAAGGAA TTTTGTAGTC 
AATTTCAAAA ATAACTCACC GAAGAAACAG TACAAGAAGT GGGTGGAATT GCCTATCAGA 
TTTCCTGATC TTGACTACTC AGAATACTGC TTGTATAGTG ATGAAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157949.1
CDS	326..3256
Translation	MSIVCSAEDSFRNLILFFRPRLKMYIQVEPVLDHLIFLSAETKEQILKKINTCGNTSAAELLLSTLEQGQWPLGWTQMFVEALEHSGNPLAARYVKPTLTDLPSPSSETAHDECLHLLTLLQPTLVDKLLINDVLDTCFEKGLLTVEDRNRISAAGNSGNESGVRELLRRIVQKENWFSTFLDVLRQTGNDALFQELTGGGCPEDNTESDTSLAEGSVSCFDESLGHNSNMGRDSGTMGSDSDESVIQTKRVSPEPELQLRPYQMEVAQPALDGKNIIICLPTGSGKTRVAVYITKDHLDKKKQASESGKVIVLVNKVMLAEQLFRKEFNPYLKKWYRIIGLSGDTQLKISFPEVVKSYDVIISTAQILENSLLNLESGDDDGVQLSDFSLIIIDECHHTNKEAVYNNIMRRYLKQKLRNNDLKKQNKPAIPLPQILGLTASPGVGAAKKQSEAEKHILNICANLDAFTIKTVKENLGQLKHQIKEPCKKFVIADDTRENPFKEKLLEIMASIQTYCQKSPMSDFGTQHYEQWAIQMEKKAAKDGNRKDRVCAEHLRKYNEALQINDTIRMIDAYSHLETFYTDEKEKKFAVLNDSDKSDDEASSCNDQLKGDVKKSLKLDETDEFLMNLFFDNKKMLKKLAENPKYENEKLIKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEVGVKAHHLIGAGHSSEVKPMTQTEQKEVISKFRTGEINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVTSSGSGVTEREIVNDFREKMMYKAINRVQNMKPEEYAHKILELQVQSILEKKMKVKRSIAKQYNDNPSLITLLCKNCSMLVCSGENIHVIEKMHHVNMTPEFKGLYIVRENKALQKKFADYQTNGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVNFKNNSPKKQYKKWVELPIRFPDLDYSEYCLYSDED

Target ORF information:

RefSeq Version	NM_001164477.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164477.1

ORF Insert Sequence:

ATGTCGATTG TCTGTTCTGC AGAGGACAGC TTCAGGAATC TCATCTTATT CTTCAGGCCC 
AGGCTGAAAA TGTACATTCA GGTGGAGCCA GTGCTGGACC ACCTCATCTT TCTGTCTGCA 
GAAACCAAAG AGCAGATTCT TAAAAAGATC AACACCTGTG GTAACACCAG CGCGGCAGAA 
CTGCTGCTGA GCACCTTGGA GCAGGGACAA TGGCCTCTGG GATGGACGCA GATGTTCGTG 
GAGGCCCTAG AGCACAGTGG CAATCCCCTA GCCGCGCGCT ATGTCAAACC CACACTCACT 
GATCTGCCCT CTCCTTCCTC TGAGACTGCC CATGACGAGT GTCTCCACTT GCTGACCCTC 
CTCCAGCCCA CTTTGGTGGA CAAACTTCTG ATTAACGATG TCTTGGACAC TTGCTTCGAG 
AAGGGACTAT TGACAGTCGA AGACAGAAAT CGGATTTCTG CTGCAGGAAA CAGCGGGAAT 
GAGTCAGGTG TAAGAGAGCT GCTGAGAAGG ATTGTGCAGA AGGAAAACTG GTTTTCTACC 
TTCCTGGATG TTCTGCGCCA AACTGGAAAT GATGCACTAT TCCAAGAACT AACAGGTGGA 
GGCTGCCCAG AAGACAACAC AGAATCAGAC ACAAGTTTGG CAGAAGGAAG TGTCAGCTGC 
TTCGATGAAA GTCTTGGACA TAACAGCAAC ATGGGCAGGG ATTCAGGCAC CATGGGAAGT 
GATTCAGATG AAAGTGTCAT CCAGACAAAA AGAGTATCCC CCGAGCCAGA ACTGCAGCTC 
AGGCCTTACC AAATGGAAGT GGCCCAACCA GCTCTAGATG GGAAGAATAT TATTATCTGC 
CTCCCCACGG GGAGTGGGAA AACCAGAGTG GCTGTTTACA TCACCAAAGA TCACTTAGAC 
AAGAAGAAGC AGGCATCTGA ATCCGGGAAG GTTATCGTTC TTGTCAATAA GGTAATGTTA 
GCAGAACAAC TTTTCCGAAA AGAGTTCAAC CCATATTTGA AGAAATGGTA TCGAATTATT 
GGATTAAGTG GCGATACCCA GCTGAAAATA TCATTTCCAG AAGTTGTCAA ATCTTACGAT 
GTTATTATCA GCACTGCTCA AATCCTTGAA AACTCCCTCT TAAATCTGGA GAGTGGAGAC 
GATGACGGTG TGCAGCTGTC AGACTTCTCT CTCATTATCA TTGATGAGTG CCATCACACC 
AACAAGGAGG CAGTCTATAA CAACATCATG AGACGATATT TGAAGCAGAA GCTGAGAAAC 
AATGACCTCA AGAAACAAAA CAAACCAGCC ATTCCCCTGC CGCAGATACT AGGACTGACA 
GCTTCACCTG GTGTTGGAGC AGCCAAAAAG CAGTCTGAGG CTGAAAAACA TATTTTAAAT 
ATATGTGCCA ATCTTGATGC CTTTACCATT AAAACAGTGA AAGAGAATCT TGGTCAACTC 
AAACACCAAA TAAAGGAACC ATGCAAGAAA TTTGTGATTG CTGATGACAC CAGAGAAAAT 
CCATTTAAAG AGAAACTTCT AGAAATTATG GCAAGCATTC AGACTTACTG CCAAAAAAGT 
CCAATGTCAG ATTTTGGAAC CCAACATTAT GAGCAGTGGG CCATTCAAAT GGAGAAAAAA 
GCTGCTAAAG ACGGAAATCG CAAAGATCGC GTCTGTGCAG AACATTTGAG GAAGTACAAC 
GAAGCCCTAC AAATCAACGA CACGATCCGA ATGATTGATG CATATAGCCA CCTGGAGACA 
TTCTACACTG ATGAGAAAGA AAAGAAGTTC GCAGTCCTCA ATGACAGCGA CAAGAGTGAT 
GACGAGGCCA GCAGTTGCAA TGACCAACTT AAGGGCGATG TAAAGAAATC TTTGAAACTG 
GACGAAACGG ATGAATTTCT CATGAATTTG TTCTTTGATA ACAAGAAAAT GTTGAAAAAA 
CTAGCTGAAA ACCCAAAATA CGAGAATGAA AAACTCATTA AATTAAGAAA CACGATACTG 
GAACAATTCA CAAGGTCTGA GGAGTCCTCC CGAGGAATTA TTTTTACAAA AACACGACAG 
AGCACCTACG CACTTTCCCA GTGGATCATG GAAAATGCAA AGTTTGCGGA AGTTGGAGTC 
AAAGCGCATC ACCTGATTGG CGCGGGGCAC AGCAGTGAAG TCAAGCCCAT GACTCAGACT 
GAACAAAAAG AAGTCATTAG TAAATTTCGC ACTGGCGAAA TAAATCTGCT TATCGCTACG 
ACGGTGGCAG AGGAAGGCCT GGATATCAAA GAGTGCAATA TTGTTATTCG TTATGGCCTT 
GTCACGAACG AGATAGCCAT GGTCCAGGCC CGGGGTCGAG CCAGAGCTGA TGAAAGCACG 
TATGTCCTGG TCACCAGCAG TGGCTCAGGA GTTACCGAAC GGGAGATTGT TAATGATTTC 
CGAGAGAAGA TGATGTATAA AGCTATTAAC CGTGTTCAAA ACATGAAACC AGAGGAGTAT 
GCACATAAGA TTTTGGAATT GCAGGTGCAA AGTATCCTGG AAAAGAAAAT GAAAGTCAAA 
AGAAGCATTG CAAAGCAATA CAACGACAAT CCATCGTTAA TAACACTTCT CTGCAAAAAT 
TGTAGCATGC TGGTCTGCTC GGGAGAAAAC ATCCATGTCA TTGAGAAGAT GCATCATGTC 
AATATGACAC CAGAATTCAA GGGACTCTAC ATTGTAAGAG AAAACAAAGC ACTGCAAAAG 
AAATTTGCTG ATTATCAGAC CAATGGAGAG ATTATCTGCA AGTGTGGCCA GGCTTGGGGA 
ACAATGATGG TGCACAAAGG TTTAGATTTG CCTTGTCTTA AAATAAGGAA TTTTGTAGTC 
AATTTCAAAA ATAACTCACC GAAGAAACAG TACAAGAAGT GGGTGGAATT GCCTATCAGA 
TTTCCTGATC TTGACTACTC AGAATACTGC TTGTATAGTG ATGAAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ifih1 ( XM_006500187.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500250.1 Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30898
Clone ID Related Accession (Same CDS sequence)	XM_006500187.4 , XM_006500187.3
Accession Version	XM_006500187.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	interferon-induced helicase C domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500187.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGGG ATTCAGGCAC CATGGGAAGT GATTCAGATG AAAGTGTCAT CCAGACAAAA 
AGAGTATCCC CCGAGCCAGA ACTGCAGCTC AGGCCTTACC AAATGGAAGT GGCCCAACCA 
GCTCTAGATG GGAAGAATAT TATTATCTGC CTCCCCACGG GGAGTGGGAA AACCAGAGTG 
GCTGTTTACA TCACCAAAGA TCACTTAGAC AAGAAGAAGC AGGCATCTGA ATCCGGGAAG 
GTTATCGTTC TTGTCAATAA GGTAATGTTA GCAGAACAAC TTTTCCGAAA AGAGTTCAAC 
CCATATTTGA AGAAATGGTA TCGAATTATT GGATTAAGTG GCGATACCCA GCTGAAAATA 
TCATTTCCAG AAGTTGTCAA ATCTTACGAT GTTATTATCA GCACTGCTCA AATCCTTGAA 
AACTCCCTCT TAAATCTGGA GAGTGGAGAC GATGACGGTG TGCAGCTGTC AGACTTCTCT 
CTCATTATCA TTGATGAGTG CCATCACACC AACAAGGAGG CAGTCTATAA CAACATCATG 
AGACGATATT TGAAGCAGAA GCTGAGAAAC AATGACCTCA AGAAACAAAA CAAACCAGCC 
ATTCCCCTGC CGCAGATACT AGGACTGACA GCTTCACCTG GTGTTGGAGC AGCCAAAAAG 
CAGTCTGAGG CTGAAAAACA TATTTTAAAT ATATGTGCCA ATCTTGATGC CTTTACCATT 
AAAACAGTGA AAGAGAATCT TGGTCAACTC AAACACCAAA TAAAGGAACC ATGCAAGAAA 
TTTGTGATTG CTGATGACAC CAGAGAAAAT CCATTTAAAG AGAAACTTCT AGAAATTATG 
GCAAGCATTC AGACTTACTG CCAAAAAAGT CCAATGTCAG ATTTTGGAAC CCAACATTAT 
GAGCAGTGGG CCATTCAAAT GGAGAAAAAA GCTGCTAAAG ACGGAAATCG CAAAGATCGC 
GTCTGTGCAG AACATTTGAG GAAGTACAAC GAAGCCCTAC AAATCAACGA CACGATCCGA 
ATGATTGATG CATATAGCCA CCTGGAGACA TTCTACACTG ATGAGAAAGA AAAGAAGTTC 
GCAGTCCTCA ATGACAGCGA CAAGAGTGAT GACGAGGCCA GCAGTTGCAA TGACCAACTT 
AAGGGCGATG TAAAGAAATC TTTGAAACTG GACGAAACGG ATGAATTTCT CATGAATTTG 
TTCTTTGATA ACAAGAAAAT GTTGAAAAAA CTAGCTGAAA ACCCAAAATA CGAGAATGAA 
AAACTCATTA AATTAAGAAA CACGATACTG GAACAATTCA CAAGGTCTGA GGAGTCCTCC 
CGAGGAATTA TTTTTACAAA AACACGACAG AGCACCTACG CACTTTCCCA GTGGATCATG 
GAAAATGCAA AGTTTGCGGA AGTTGGAGTC AAAGCGCATC ACCTGATTGG CGCGGGGCAC 
AGCAGTGAAG TCAAGCCCAT GACTCAGACT GAACAAAAAG AAGTCATTAG TAAATTTCGC 
ACTGGCGAAA TAAATCTGCT TATCGCTACG ACGGTGGCAG AGGAAGGCCT GGATATCAAA 
GAGTGCAATA TTGTTATTCG TTATGGCCTT GTCACGAACG AGATAGCCAT GGTCCAGGCC 
CGGGGTCGAG CCAGAGCTGA TGAAAGCACG TATGTCCTGG TCACCAGCAG TGGCTCAGGA 
GTTACCGAAC GGGAGATTGT TAATGATTTC CGAGAGAAGA TGATGTATAA AGCTATTAAC 
CGTGTTCAAA ACATGAAACC AGAGGAGTAT GCACATAAGA TTTTGGAATT GCAGGTGCAA 
AGTATCCTGG AAAAGAAAAT GAAAGTCAAA AGAAGCATTG CAAAGCAATA CAACGACAAT 
CCATCGTTAA TAACACTTCT CTGCAAAAAT TGTAGCATGC TGGTCTGCTC GGGAGAAAAC 
ATCCATGTCA TTGAGAAGAT GCATCATGTC AATATGACAC CAGAATTCAA GGGACTCTAC 
ATTGTAAGAG AAAACAAAGC ACTGCAAAAG AAATTTGCTG ATTATCAGAC CAATGGAGAG 
ATTATCTGCA AGTGTGGCCA GGCTTGGGGA ACAATGATGG TGCACAAAGG TTTAGATTTG 
CCTTGTCTTA AAATAAGGAA TTTTGTAGTC AATTTCAAAA ATAACTCACC GAAGAAACAG 
TACAAGAAGT GGGTGGAATT GCCTATCAGA TTTCCTGATC TTGACTACTC AGAATACTGC 
TTGTATAGTG ATGAAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500250.1
CDS	188..2428
Translation	MGRDSGTMGSDSDESVIQTKRVSPEPELQLRPYQMEVAQPALDGKNIIICLPTGSGKTRVAVYITKDHLDKKKQASESGKVIVLVNKVMLAEQLFRKEFNPYLKKWYRIIGLSGDTQLKISFPEVVKSYDVIISTAQILENSLLNLESGDDDGVQLSDFSLIIIDECHHTNKEAVYNNIMRRYLKQKLRNNDLKKQNKPAIPLPQILGLTASPGVGAAKKQSEAEKHILNICANLDAFTIKTVKENLGQLKHQIKEPCKKFVIADDTRENPFKEKLLEIMASIQTYCQKSPMSDFGTQHYEQWAIQMEKKAAKDGNRKDRVCAEHLRKYNEALQINDTIRMIDAYSHLETFYTDEKEKKFAVLNDSDKSDDEASSCNDQLKGDVKKSLKLDETDEFLMNLFFDNKKMLKKLAENPKYENEKLIKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEVGVKAHHLIGAGHSSEVKPMTQTEQKEVISKFRTGEINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVTSSGSGVTEREIVNDFREKMMYKAINRVQNMKPEEYAHKILELQVQSILEKKMKVKRSIAKQYNDNPSLITLLCKNCSMLVCSGENIHVIEKMHHVNMTPEFKGLYIVRENKALQKKFADYQTNGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVNFKNNSPKKQYKKWVELPIRFPDLDYSEYCLYSDED

Target ORF information:

RefSeq Version	XM_006500187.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500187.4

ORF Insert Sequence:

ATGGGCAGGG ATTCAGGCAC CATGGGAAGT GATTCAGATG AAAGTGTCAT CCAGACAAAA 
AGAGTATCCC CCGAGCCAGA ACTGCAGCTC AGGCCTTACC AAATGGAAGT GGCCCAACCA 
GCTCTAGATG GGAAGAATAT TATTATCTGC CTCCCCACGG GGAGTGGGAA AACCAGAGTG 
GCTGTTTACA TCACCAAAGA TCACTTAGAC AAGAAGAAGC AGGCATCTGA ATCCGGGAAG 
GTTATCGTTC TTGTCAATAA GGTAATGTTA GCAGAACAAC TTTTCCGAAA AGAGTTCAAC 
CCATATTTGA AGAAATGGTA TCGAATTATT GGATTAAGTG GCGATACCCA GCTGAAAATA 
TCATTTCCAG AAGTTGTCAA ATCTTACGAT GTTATTATCA GCACTGCTCA AATCCTTGAA 
AACTCCCTCT TAAATCTGGA GAGTGGAGAC GATGACGGTG TGCAGCTGTC AGACTTCTCT 
CTCATTATCA TTGATGAGTG CCATCACACC AACAAGGAGG CAGTCTATAA CAACATCATG 
AGACGATATT TGAAGCAGAA GCTGAGAAAC AATGACCTCA AGAAACAAAA CAAACCAGCC 
ATTCCCCTGC CGCAGATACT AGGACTGACA GCTTCACCTG GTGTTGGAGC AGCCAAAAAG 
CAGTCTGAGG CTGAAAAACA TATTTTAAAT ATATGTGCCA ATCTTGATGC CTTTACCATT 
AAAACAGTGA AAGAGAATCT TGGTCAACTC AAACACCAAA TAAAGGAACC ATGCAAGAAA 
TTTGTGATTG CTGATGACAC CAGAGAAAAT CCATTTAAAG AGAAACTTCT AGAAATTATG 
GCAAGCATTC AGACTTACTG CCAAAAAAGT CCAATGTCAG ATTTTGGAAC CCAACATTAT 
GAGCAGTGGG CCATTCAAAT GGAGAAAAAA GCTGCTAAAG ACGGAAATCG CAAAGATCGC 
GTCTGTGCAG AACATTTGAG GAAGTACAAC GAAGCCCTAC AAATCAACGA CACGATCCGA 
ATGATTGATG CATATAGCCA CCTGGAGACA TTCTACACTG ATGAGAAAGA AAAGAAGTTC 
GCAGTCCTCA ATGACAGCGA CAAGAGTGAT GACGAGGCCA GCAGTTGCAA TGACCAACTT 
AAGGGCGATG TAAAGAAATC TTTGAAACTG GACGAAACGG ATGAATTTCT CATGAATTTG 
TTCTTTGATA ACAAGAAAAT GTTGAAAAAA CTAGCTGAAA ACCCAAAATA CGAGAATGAA 
AAACTCATTA AATTAAGAAA CACGATACTG GAACAATTCA CAAGGTCTGA GGAGTCCTCC 
CGAGGAATTA TTTTTACAAA AACACGACAG AGCACCTACG CACTTTCCCA GTGGATCATG 
GAAAATGCAA AGTTTGCGGA AGTTGGAGTC AAAGCGCATC ACCTGATTGG CGCGGGGCAC 
AGCAGTGAAG TCAAGCCCAT GACTCAGACT GAACAAAAAG AAGTCATTAG TAAATTTCGC 
ACTGGCGAAA TAAATCTGCT TATCGCTACG ACGGTGGCAG AGGAAGGCCT GGATATCAAA 
GAGTGCAATA TTGTTATTCG TTATGGCCTT GTCACGAACG AGATAGCCAT GGTCCAGGCC 
CGGGGTCGAG CCAGAGCTGA TGAAAGCACG TATGTCCTGG TCACCAGCAG TGGCTCAGGA 
GTTACCGAAC GGGAGATTGT TAATGATTTC CGAGAGAAGA TGATGTATAA AGCTATTAAC 
CGTGTTCAAA ACATGAAACC AGAGGAGTAT GCACATAAGA TTTTGGAATT GCAGGTGCAA 
AGTATCCTGG AAAAGAAAAT GAAAGTCAAA AGAAGCATTG CAAAGCAATA CAACGACAAT 
CCATCGTTAA TAACACTTCT CTGCAAAAAT TGTAGCATGC TGGTCTGCTC GGGAGAAAAC 
ATCCATGTCA TTGAGAAGAT GCATCATGTC AATATGACAC CAGAATTCAA GGGACTCTAC 
ATTGTAAGAG AAAACAAAGC ACTGCAAAAG AAATTTGCTG ATTATCAGAC CAATGGAGAG 
ATTATCTGCA AGTGTGGCCA GGCTTGGGGA ACAATGATGG TGCACAAAGG TTTAGATTTG 
CCTTGTCTTA AAATAAGGAA TTTTGTAGTC AATTTCAAAA ATAACTCACC GAAGAAACAG 
TACAAGAAGT GGGTGGAATT GCCTATCAGA TTTCCTGATC TTGACTACTC AGAATACTGC 
TTGTATAGTG ATGAAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ifih1 ( XM_006500187.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500250.1 Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant X1, mRNA.

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CloneID	OMu30898
Clone ID Related Accession (Same CDS sequence)	XM_006500187.4 , XM_006500187.3
Accession Version	XM_006500187.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	interferon-induced helicase C domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500187.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGGG ATTCAGGCAC CATGGGAAGT GATTCAGATG AAAGTGTCAT CCAGACAAAA 
AGAGTATCCC CCGAGCCAGA ACTGCAGCTC AGGCCTTACC AAATGGAAGT GGCCCAACCA 
GCTCTAGATG GGAAGAATAT TATTATCTGC CTCCCCACGG GGAGTGGGAA AACCAGAGTG 
GCTGTTTACA TCACCAAAGA TCACTTAGAC AAGAAGAAGC AGGCATCTGA ATCCGGGAAG 
GTTATCGTTC TTGTCAATAA GGTAATGTTA GCAGAACAAC TTTTCCGAAA AGAGTTCAAC 
CCATATTTGA AGAAATGGTA TCGAATTATT GGATTAAGTG GCGATACCCA GCTGAAAATA 
TCATTTCCAG AAGTTGTCAA ATCTTACGAT GTTATTATCA GCACTGCTCA AATCCTTGAA 
AACTCCCTCT TAAATCTGGA GAGTGGAGAC GATGACGGTG TGCAGCTGTC AGACTTCTCT 
CTCATTATCA TTGATGAGTG CCATCACACC AACAAGGAGG CAGTCTATAA CAACATCATG 
AGACGATATT TGAAGCAGAA GCTGAGAAAC AATGACCTCA AGAAACAAAA CAAACCAGCC 
ATTCCCCTGC CGCAGATACT AGGACTGACA GCTTCACCTG GTGTTGGAGC AGCCAAAAAG 
CAGTCTGAGG CTGAAAAACA TATTTTAAAT ATATGTGCCA ATCTTGATGC CTTTACCATT 
AAAACAGTGA AAGAGAATCT TGGTCAACTC AAACACCAAA TAAAGGAACC ATGCAAGAAA 
TTTGTGATTG CTGATGACAC CAGAGAAAAT CCATTTAAAG AGAAACTTCT AGAAATTATG 
GCAAGCATTC AGACTTACTG CCAAAAAAGT CCAATGTCAG ATTTTGGAAC CCAACATTAT 
GAGCAGTGGG CCATTCAAAT GGAGAAAAAA GCTGCTAAAG ACGGAAATCG CAAAGATCGC 
GTCTGTGCAG AACATTTGAG GAAGTACAAC GAAGCCCTAC AAATCAACGA CACGATCCGA 
ATGATTGATG CATATAGCCA CCTGGAGACA TTCTACACTG ATGAGAAAGA AAAGAAGTTC 
GCAGTCCTCA ATGACAGCGA CAAGAGTGAT GACGAGGCCA GCAGTTGCAA TGACCAACTT 
AAGGGCGATG TAAAGAAATC TTTGAAACTG GACGAAACGG ATGAATTTCT CATGAATTTG 
TTCTTTGATA ACAAGAAAAT GTTGAAAAAA CTAGCTGAAA ACCCAAAATA CGAGAATGAA 
AAACTCATTA AATTAAGAAA CACGATACTG GAACAATTCA CAAGGTCTGA GGAGTCCTCC 
CGAGGAATTA TTTTTACAAA AACACGACAG AGCACCTACG CACTTTCCCA GTGGATCATG 
GAAAATGCAA AGTTTGCGGA AGTTGGAGTC AAAGCGCATC ACCTGATTGG CGCGGGGCAC 
AGCAGTGAAG TCAAGCCCAT GACTCAGACT GAACAAAAAG AAGTCATTAG TAAATTTCGC 
ACTGGCGAAA TAAATCTGCT TATCGCTACG ACGGTGGCAG AGGAAGGCCT GGATATCAAA 
GAGTGCAATA TTGTTATTCG TTATGGCCTT GTCACGAACG AGATAGCCAT GGTCCAGGCC 
CGGGGTCGAG CCAGAGCTGA TGAAAGCACG TATGTCCTGG TCACCAGCAG TGGCTCAGGA 
GTTACCGAAC GGGAGATTGT TAATGATTTC CGAGAGAAGA TGATGTATAA AGCTATTAAC 
CGTGTTCAAA ACATGAAACC AGAGGAGTAT GCACATAAGA TTTTGGAATT GCAGGTGCAA 
AGTATCCTGG AAAAGAAAAT GAAAGTCAAA AGAAGCATTG CAAAGCAATA CAACGACAAT 
CCATCGTTAA TAACACTTCT CTGCAAAAAT TGTAGCATGC TGGTCTGCTC GGGAGAAAAC 
ATCCATGTCA TTGAGAAGAT GCATCATGTC AATATGACAC CAGAATTCAA GGGACTCTAC 
ATTGTAAGAG AAAACAAAGC ACTGCAAAAG AAATTTGCTG ATTATCAGAC CAATGGAGAG 
ATTATCTGCA AGTGTGGCCA GGCTTGGGGA ACAATGATGG TGCACAAAGG TTTAGATTTG 
CCTTGTCTTA AAATAAGGAA TTTTGTAGTC AATTTCAAAA ATAACTCACC GAAGAAACAG 
TACAAGAAGT GGGTGGAATT GCCTATCAGA TTTCCTGATC TTGACTACTC AGAATACTGC 
TTGTATAGTG ATGAAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500250.1
CDS	188..2428
Translation	MGRDSGTMGSDSDESVIQTKRVSPEPELQLRPYQMEVAQPALDGKNIIICLPTGSGKTRVAVYITKDHLDKKKQASESGKVIVLVNKVMLAEQLFRKEFNPYLKKWYRIIGLSGDTQLKISFPEVVKSYDVIISTAQILENSLLNLESGDDDGVQLSDFSLIIIDECHHTNKEAVYNNIMRRYLKQKLRNNDLKKQNKPAIPLPQILGLTASPGVGAAKKQSEAEKHILNICANLDAFTIKTVKENLGQLKHQIKEPCKKFVIADDTRENPFKEKLLEIMASIQTYCQKSPMSDFGTQHYEQWAIQMEKKAAKDGNRKDRVCAEHLRKYNEALQINDTIRMIDAYSHLETFYTDEKEKKFAVLNDSDKSDDEASSCNDQLKGDVKKSLKLDETDEFLMNLFFDNKKMLKKLAENPKYENEKLIKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEVGVKAHHLIGAGHSSEVKPMTQTEQKEVISKFRTGEINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVTSSGSGVTEREIVNDFREKMMYKAINRVQNMKPEEYAHKILELQVQSILEKKMKVKRSIAKQYNDNPSLITLLCKNCSMLVCSGENIHVIEKMHHVNMTPEFKGLYIVRENKALQKKFADYQTNGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVNFKNNSPKKQYKKWVELPIRFPDLDYSEYCLYSDED

Target ORF information:

RefSeq Version	XM_006500187.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500187.3

ORF Insert Sequence:

ATGGGCAGGG ATTCAGGCAC CATGGGAAGT GATTCAGATG AAAGTGTCAT CCAGACAAAA 
AGAGTATCCC CCGAGCCAGA ACTGCAGCTC AGGCCTTACC AAATGGAAGT GGCCCAACCA 
GCTCTAGATG GGAAGAATAT TATTATCTGC CTCCCCACGG GGAGTGGGAA AACCAGAGTG 
GCTGTTTACA TCACCAAAGA TCACTTAGAC AAGAAGAAGC AGGCATCTGA ATCCGGGAAG 
GTTATCGTTC TTGTCAATAA GGTAATGTTA GCAGAACAAC TTTTCCGAAA AGAGTTCAAC 
CCATATTTGA AGAAATGGTA TCGAATTATT GGATTAAGTG GCGATACCCA GCTGAAAATA 
TCATTTCCAG AAGTTGTCAA ATCTTACGAT GTTATTATCA GCACTGCTCA AATCCTTGAA 
AACTCCCTCT TAAATCTGGA GAGTGGAGAC GATGACGGTG TGCAGCTGTC AGACTTCTCT 
CTCATTATCA TTGATGAGTG CCATCACACC AACAAGGAGG CAGTCTATAA CAACATCATG 
AGACGATATT TGAAGCAGAA GCTGAGAAAC AATGACCTCA AGAAACAAAA CAAACCAGCC 
ATTCCCCTGC CGCAGATACT AGGACTGACA GCTTCACCTG GTGTTGGAGC AGCCAAAAAG 
CAGTCTGAGG CTGAAAAACA TATTTTAAAT ATATGTGCCA ATCTTGATGC CTTTACCATT 
AAAACAGTGA AAGAGAATCT TGGTCAACTC AAACACCAAA TAAAGGAACC ATGCAAGAAA 
TTTGTGATTG CTGATGACAC CAGAGAAAAT CCATTTAAAG AGAAACTTCT AGAAATTATG 
GCAAGCATTC AGACTTACTG CCAAAAAAGT CCAATGTCAG ATTTTGGAAC CCAACATTAT 
GAGCAGTGGG CCATTCAAAT GGAGAAAAAA GCTGCTAAAG ACGGAAATCG CAAAGATCGC 
GTCTGTGCAG AACATTTGAG GAAGTACAAC GAAGCCCTAC AAATCAACGA CACGATCCGA 
ATGATTGATG CATATAGCCA CCTGGAGACA TTCTACACTG ATGAGAAAGA AAAGAAGTTC 
GCAGTCCTCA ATGACAGCGA CAAGAGTGAT GACGAGGCCA GCAGTTGCAA TGACCAACTT 
AAGGGCGATG TAAAGAAATC TTTGAAACTG GACGAAACGG ATGAATTTCT CATGAATTTG 
TTCTTTGATA ACAAGAAAAT GTTGAAAAAA CTAGCTGAAA ACCCAAAATA CGAGAATGAA 
AAACTCATTA AATTAAGAAA CACGATACTG GAACAATTCA CAAGGTCTGA GGAGTCCTCC 
CGAGGAATTA TTTTTACAAA AACACGACAG AGCACCTACG CACTTTCCCA GTGGATCATG 
GAAAATGCAA AGTTTGCGGA AGTTGGAGTC AAAGCGCATC ACCTGATTGG CGCGGGGCAC 
AGCAGTGAAG TCAAGCCCAT GACTCAGACT GAACAAAAAG AAGTCATTAG TAAATTTCGC 
ACTGGCGAAA TAAATCTGCT TATCGCTACG ACGGTGGCAG AGGAAGGCCT GGATATCAAA 
GAGTGCAATA TTGTTATTCG TTATGGCCTT GTCACGAACG AGATAGCCAT GGTCCAGGCC 
CGGGGTCGAG CCAGAGCTGA TGAAAGCACG TATGTCCTGG TCACCAGCAG TGGCTCAGGA 
GTTACCGAAC GGGAGATTGT TAATGATTTC CGAGAGAAGA TGATGTATAA AGCTATTAAC 
CGTGTTCAAA ACATGAAACC AGAGGAGTAT GCACATAAGA TTTTGGAATT GCAGGTGCAA 
AGTATCCTGG AAAAGAAAAT GAAAGTCAAA AGAAGCATTG CAAAGCAATA CAACGACAAT 
CCATCGTTAA TAACACTTCT CTGCAAAAAT TGTAGCATGC TGGTCTGCTC GGGAGAAAAC 
ATCCATGTCA TTGAGAAGAT GCATCATGTC AATATGACAC CAGAATTCAA GGGACTCTAC 
ATTGTAAGAG AAAACAAAGC ACTGCAAAAG AAATTTGCTG ATTATCAGAC CAATGGAGAG 
ATTATCTGCA AGTGTGGCCA GGCTTGGGGA ACAATGATGG TGCACAAAGG TTTAGATTTG 
CCTTGTCTTA AAATAAGGAA TTTTGTAGTC AATTTCAAAA ATAACTCACC GAAGAAACAG 
TACAAGAAGT GGGTGGAATT GCCTATCAGA TTTCCTGATC TTGACTACTC AGAATACTGC 
TTGTATAGTG ATGAAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ifih1 ( NM_027835.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082111.2 Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant 1, mRNA.

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CloneID	OMu21680
Clone ID Related Accession (Same CDS sequence)	NM_027835.3
Accession Version	NM_027835.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	interferon-induced helicase C domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929246.7, AK168646.1 and AY075132.1. On Sep 2, 2009 this sequence version replaced NM_027835.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153018.1, AF374384.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGATTG TCTGTTCTGC AGAGGACAGC TTCAGGAATC TCATCTTATT CTTCAGGCCC 
AGGCTGAAAA TGTACATTCA GGTGGAGCCA GTGCTGGACC ACCTCATCTT TCTGTCTGCA 
GAAACCAAAG AGCAGATTCT TAAAAAGATC AACACCTGTG GTAACACCAG CGCGGCAGAA 
CTGCTGCTGA GCACCTTGGA GCAGGGACAA TGGCCTCTGG GATGGACGCA GATGTTCGTG 
GAGGCCCTAG AGCACAGTGG CAATCCCCTA GCCGCGCGCT ATGTCAAACC CACACTCACT 
GATCTGCCCT CTCCTTCCTC TGAGACTGCC CATGACGAGT GTCTCCACTT GCTGACCCTC 
CTCCAGCCCA CTTTGGTGGA CAAACTTCTG ATTAACGATG TCTTGGACAC TTGCTTCGAG 
AAGGGACTAT TGACAGTCGA AGACAGAAAT CGGATTTCTG CTGCAGGAAA CAGCGGGAAT 
GAGTCAGGTG TAAGAGAGCT GCTGAGAAGG ATTGTGCAGA AGGAAAACTG GTTTTCTACC 
TTCCTGGATG TTCTGCGCCA AACTGGAAAT GATGCACTAT TCCAAGAACT AACAGGTGGA 
GGCTGCCCAG AAGACAACAC AGACTTGGCT AACTCGTCTC ACAGAGATGG GCCTGCAGCT 
AATGAGTGTC TTCTGCCTGC TGTCGATGAG TCAAGTCTGG AGACAGAGGC CTGGAACGTA 
GACGACATAT TACCAGAGGC TTCTTGTACA GATTCGTCTG TGACCACAGA ATCAGACACA 
AGTTTGGCAG AAGGAAGTGT CAGCTGCTTC GATGAAAGTC TTGGACATAA CAGCAACATG 
GGCAGGGATT CAGGCACCAT GGGAAGTGAT TCAGATGAAA GTGTCATCCA GACAAAAAGA 
GTATCCCCCG AGCCAGAACT GCAGCTCAGG CCTTACCAAA TGGAAGTGGC CCAACCAGCT 
CTAGATGGGA AGAATATTAT TATCTGCCTC CCCACGGGGA GTGGGAAAAC CAGAGTGGCT 
GTTTACATCA CCAAAGATCA CTTAGACAAG AAGAAGCAGG CATCTGAATC CGGGAAGGTT 
ATCGTTCTTG TCAATAAGGT AATGTTAGCA GAACAACTTT TCCGAAAAGA GTTCAACCCA 
TATTTGAAGA AATGGTATCG AATTATTGGA TTAAGTGGCG ATACCCAGCT GAAAATATCA 
TTTCCAGAAG TTGTCAAATC TTACGATGTT ATTATCAGCA CTGCTCAAAT CCTTGAAAAC 
TCCCTCTTAA ATCTGGAGAG TGGAGACGAT GACGGTGTGC AGCTGTCAGA CTTCTCTCTC 
ATTATCATTG ATGAGTGCCA TCACACCAAC AAGGAGGCAG TCTATAACAA CATCATGAGA 
CGATATTTGA AGCAGAAGCT GAGAAACAAT GACCTCAAGA AACAAAACAA ACCAGCCATT 
CCCCTGCCGC AGATACTAGG ACTGACAGCT TCACCTGGTG TTGGAGCAGC CAAAAAGCAG 
TCTGAGGCTG AAAAACATAT TTTAAATATA TGTGCCAATC TTGATGCCTT TACCATTAAA 
ACAGTGAAAG AGAATCTTGG TCAACTCAAA CACCAAATAA AGGAACCATG CAAGAAATTT 
GTGATTGCTG ATGACACCAG AGAAAATCCA TTTAAAGAGA AACTTCTAGA AATTATGGCA 
AGCATTCAGA CTTACTGCCA AAAAAGTCCA ATGTCAGATT TTGGAACCCA ACATTATGAG 
CAGTGGGCCA TTCAAATGGA GAAAAAAGCT GCTAAAGACG GAAATCGCAA AGATCGCGTC 
TGTGCAGAAC ATTTGAGGAA GTACAACGAA GCCCTACAAA TCAACGACAC GATCCGAATG 
ATTGATGCAT ATAGCCACCT GGAGACATTC TACACTGATG AGAAAGAAAA GAAGTTCGCA 
GTCCTCAATG ACAGCGACAA GAGTGATGAC GAGGCCAGCA GTTGCAATGA CCAACTTAAG 
GGCGATGTAA AGAAATCTTT GAAACTGGAC GAAACGGATG AATTTCTCAT GAATTTGTTC 
TTTGATAACA AGAAAATGTT GAAAAAACTA GCTGAAAACC CAAAATACGA GAATGAAAAA 
CTCATTAAAT TAAGAAACAC GATACTGGAA CAATTCACAA GGTCTGAGGA GTCCTCCCGA 
GGAATTATTT TTACAAAAAC ACGACAGAGC ACCTACGCAC TTTCCCAGTG GATCATGGAA 
AATGCAAAGT TTGCGGAAGT TGGAGTCAAA GCGCATCACC TGATTGGCGC GGGGCACAGC 
AGTGAAGTCA AGCCCATGAC TCAGACTGAA CAAAAAGAAG TCATTAGTAA ATTTCGCACT 
GGCGAAATAA ATCTGCTTAT CGCTACGACG GTGGCAGAGG AAGGCCTGGA TATCAAAGAG 
TGCAATATTG TTATTCGTTA TGGCCTTGTC ACGAACGAGA TAGCCATGGT CCAGGCCCGG 
GGTCGAGCCA GAGCTGATGA AAGCACGTAT GTCCTGGTCA CCAGCAGTGG CTCAGGAGTT 
ACCGAACGGG AGATTGTTAA TGATTTCCGA GAGAAGATGA TGTATAAAGC TATTAACCGT 
GTTCAAAACA TGAAACCAGA GGAGTATGCA CATAAGATTT TGGAATTGCA GGTGCAAAGT 
ATCCTGGAAA AGAAAATGAA AGTCAAAAGA AGCATTGCAA AGCAATACAA CGACAATCCA 
TCGTTAATAA CACTTCTCTG CAAAAATTGT AGCATGCTGG TCTGCTCGGG AGAAAACATC 
CATGTCATTG AGAAGATGCA TCATGTCAAT ATGACACCAG AATTCAAGGG ACTCTACATT 
GTAAGAGAAA ACAAAGCACT GCAAAAGAAA TTTGCTGATT ATCAGACCAA TGGAGAGATT 
ATCTGCAAGT GTGGCCAGGC TTGGGGAACA ATGATGGTGC ACAAAGGTTT AGATTTGCCT 
TGTCTTAAAA TAAGGAATTT TGTAGTCAAT TTCAAAAATA ACTCACCGAA GAAACAGTAC 
AAGAAGTGGG TGGAATTGCC TATCAGATTT CCTGATCTTG ACTACTCAGA ATACTGCTTG 
TATAGTGATG AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082111.2
CDS	326..3403
Translation	MSIVCSAEDSFRNLILFFRPRLKMYIQVEPVLDHLIFLSAETKEQILKKINTCGNTSAAELLLSTLEQGQWPLGWTQMFVEALEHSGNPLAARYVKPTLTDLPSPSSETAHDECLHLLTLLQPTLVDKLLINDVLDTCFEKGLLTVEDRNRISAAGNSGNESGVRELLRRIVQKENWFSTFLDVLRQTGNDALFQELTGGGCPEDNTDLANSSHRDGPAANECLLPAVDESSLETEAWNVDDILPEASCTDSSVTTESDTSLAEGSVSCFDESLGHNSNMGRDSGTMGSDSDESVIQTKRVSPEPELQLRPYQMEVAQPALDGKNIIICLPTGSGKTRVAVYITKDHLDKKKQASESGKVIVLVNKVMLAEQLFRKEFNPYLKKWYRIIGLSGDTQLKISFPEVVKSYDVIISTAQILENSLLNLESGDDDGVQLSDFSLIIIDECHHTNKEAVYNNIMRRYLKQKLRNNDLKKQNKPAIPLPQILGLTASPGVGAAKKQSEAEKHILNICANLDAFTIKTVKENLGQLKHQIKEPCKKFVIADDTRENPFKEKLLEIMASIQTYCQKSPMSDFGTQHYEQWAIQMEKKAAKDGNRKDRVCAEHLRKYNEALQINDTIRMIDAYSHLETFYTDEKEKKFAVLNDSDKSDDEASSCNDQLKGDVKKSLKLDETDEFLMNLFFDNKKMLKKLAENPKYENEKLIKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEVGVKAHHLIGAGHSSEVKPMTQTEQKEVISKFRTGEINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVTSSGSGVTEREIVNDFREKMMYKAINRVQNMKPEEYAHKILELQVQSILEKKMKVKRSIAKQYNDNPSLITLLCKNCSMLVCSGENIHVIEKMHHVNMTPEFKGLYIVRENKALQKKFADYQTNGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVNFKNNSPKKQYKKWVELPIRFPDLDYSEYCLYSDED

Target ORF information:

RefSeq Version	NM_027835.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus interferon induced with helicase C domain 1 (Ifih1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027835.3

ORF Insert Sequence:

ATGTCGATTG TCTGTTCTGC AGAGGACAGC TTCAGGAATC TCATCTTATT CTTCAGGCCC 
AGGCTGAAAA TGTACATTCA GGTGGAGCCA GTGCTGGACC ACCTCATCTT TCTGTCTGCA 
GAAACCAAAG AGCAGATTCT TAAAAAGATC AACACCTGTG GTAACACCAG CGCGGCAGAA 
CTGCTGCTGA GCACCTTGGA GCAGGGACAA TGGCCTCTGG GATGGACGCA GATGTTCGTG 
GAGGCCCTAG AGCACAGTGG CAATCCCCTA GCCGCGCGCT ATGTCAAACC CACACTCACT 
GATCTGCCCT CTCCTTCCTC TGAGACTGCC CATGACGAGT GTCTCCACTT GCTGACCCTC 
CTCCAGCCCA CTTTGGTGGA CAAACTTCTG ATTAACGATG TCTTGGACAC TTGCTTCGAG 
AAGGGACTAT TGACAGTCGA AGACAGAAAT CGGATTTCTG CTGCAGGAAA CAGCGGGAAT 
GAGTCAGGTG TAAGAGAGCT GCTGAGAAGG ATTGTGCAGA AGGAAAACTG GTTTTCTACC 
TTCCTGGATG TTCTGCGCCA AACTGGAAAT GATGCACTAT TCCAAGAACT AACAGGTGGA 
GGCTGCCCAG AAGACAACAC AGACTTGGCT AACTCGTCTC ACAGAGATGG GCCTGCAGCT 
AATGAGTGTC TTCTGCCTGC TGTCGATGAG TCAAGTCTGG AGACAGAGGC CTGGAACGTA 
GACGACATAT TACCAGAGGC TTCTTGTACA GATTCGTCTG TGACCACAGA ATCAGACACA 
AGTTTGGCAG AAGGAAGTGT CAGCTGCTTC GATGAAAGTC TTGGACATAA CAGCAACATG 
GGCAGGGATT CAGGCACCAT GGGAAGTGAT TCAGATGAAA GTGTCATCCA GACAAAAAGA 
GTATCCCCCG AGCCAGAACT GCAGCTCAGG CCTTACCAAA TGGAAGTGGC CCAACCAGCT 
CTAGATGGGA AGAATATTAT TATCTGCCTC CCCACGGGGA GTGGGAAAAC CAGAGTGGCT 
GTTTACATCA CCAAAGATCA CTTAGACAAG AAGAAGCAGG CATCTGAATC CGGGAAGGTT 
ATCGTTCTTG TCAATAAGGT AATGTTAGCA GAACAACTTT TCCGAAAAGA GTTCAACCCA 
TATTTGAAGA AATGGTATCG AATTATTGGA TTAAGTGGCG ATACCCAGCT GAAAATATCA 
TTTCCAGAAG TTGTCAAATC TTACGATGTT ATTATCAGCA CTGCTCAAAT CCTTGAAAAC 
TCCCTCTTAA ATCTGGAGAG TGGAGACGAT GACGGTGTGC AGCTGTCAGA CTTCTCTCTC 
ATTATCATTG ATGAGTGCCA TCACACCAAC AAGGAGGCAG TCTATAACAA CATCATGAGA 
CGATATTTGA AGCAGAAGCT GAGAAACAAT GACCTCAAGA AACAAAACAA ACCAGCCATT 
CCCCTGCCGC AGATACTAGG ACTGACAGCT TCACCTGGTG TTGGAGCAGC CAAAAAGCAG 
TCTGAGGCTG AAAAACATAT TTTAAATATA TGTGCCAATC TTGATGCCTT TACCATTAAA 
ACAGTGAAAG AGAATCTTGG TCAACTCAAA CACCAAATAA AGGAACCATG CAAGAAATTT 
GTGATTGCTG ATGACACCAG AGAAAATCCA TTTAAAGAGA AACTTCTAGA AATTATGGCA 
AGCATTCAGA CTTACTGCCA AAAAAGTCCA ATGTCAGATT TTGGAACCCA ACATTATGAG 
CAGTGGGCCA TTCAAATGGA GAAAAAAGCT GCTAAAGACG GAAATCGCAA AGATCGCGTC 
TGTGCAGAAC ATTTGAGGAA GTACAACGAA GCCCTACAAA TCAACGACAC GATCCGAATG 
ATTGATGCAT ATAGCCACCT GGAGACATTC TACACTGATG AGAAAGAAAA GAAGTTCGCA 
GTCCTCAATG ACAGCGACAA GAGTGATGAC GAGGCCAGCA GTTGCAATGA CCAACTTAAG 
GGCGATGTAA AGAAATCTTT GAAACTGGAC GAAACGGATG AATTTCTCAT GAATTTGTTC 
TTTGATAACA AGAAAATGTT GAAAAAACTA GCTGAAAACC CAAAATACGA GAATGAAAAA 
CTCATTAAAT TAAGAAACAC GATACTGGAA CAATTCACAA GGTCTGAGGA GTCCTCCCGA 
GGAATTATTT TTACAAAAAC ACGACAGAGC ACCTACGCAC TTTCCCAGTG GATCATGGAA 
AATGCAAAGT TTGCGGAAGT TGGAGTCAAA GCGCATCACC TGATTGGCGC GGGGCACAGC 
AGTGAAGTCA AGCCCATGAC TCAGACTGAA CAAAAAGAAG TCATTAGTAA ATTTCGCACT 
GGCGAAATAA ATCTGCTTAT CGCTACGACG GTGGCAGAGG AAGGCCTGGA TATCAAAGAG 
TGCAATATTG TTATTCGTTA TGGCCTTGTC ACGAACGAGA TAGCCATGGT CCAGGCCCGG 
GGTCGAGCCA GAGCTGATGA AAGCACGTAT GTCCTGGTCA CCAGCAGTGG CTCAGGAGTT 
ACCGAACGGG AGATTGTTAA TGATTTCCGA GAGAAGATGA TGTATAAAGC TATTAACCGT 
GTTCAAAACA TGAAACCAGA GGAGTATGCA CATAAGATTT TGGAATTGCA GGTGCAAAGT 
ATCCTGGAAA AGAAAATGAA AGTCAAAAGA AGCATTGCAA AGCAATACAA CGACAATCCA 
TCGTTAATAA CACTTCTCTG CAAAAATTGT AGCATGCTGG TCTGCTCGGG AGAAAACATC 
CATGTCATTG AGAAGATGCA TCATGTCAAT ATGACACCAG AATTCAAGGG ACTCTACATT 
GTAAGAGAAA ACAAAGCACT GCAAAAGAAA TTTGCTGATT ATCAGACCAA TGGAGAGATT 
ATCTGCAAGT GTGGCCAGGC TTGGGGAACA ATGATGGTGC ACAAAGGTTT AGATTTGCCT 
TGTCTTAAAA TAAGGAATTT TGTAGTCAAT TTCAAAAATA ACTCACCGAA GAAACAGTAC 
AAGAAGTGGG TGGAATTGCC TATCAGATTT CCTGATCTTG ACTACTCAGA ATACTGCTTG 
TATAGTGATG AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Noncoding dsRNA induces retinoic acid synthesis to stimulate hair follicle regeneration via TLR3.
Nature communications10(1)2811(2019 06)
Kim D,Chen R,Sheu M,Kim N,Kim S,Islam N,Wier EM,Wang G,Li A,Park A,Son W,Evans B,Yu V,Prizmic VP,Oh E,Wang Z,Yu J,Huang W,Archer NK,Hu Z,Clemetson N,Nelson AM,Chien A,Okoye GA,Miller LS,Ghiaur G,Kang S,Jones JW,Kane MA,Garza LA

CCR5 is a required signaling receptor for macrophage expression of inflammatory genes in response to viral double-stranded RNA.
American journal of physiology. Regulatory, integrative and comparative physiology316(5)R525-R534(2019 05)
Shaheen ZR,Christmann BS,Stafford JD,Moran JM,Buller RML,Corbett JA

Distinct roles for MDA5 and TLR3 in the acute response to inhaled double-stranded RNA.
PloS one14(5)e0216056(2019)
Veazey JM,Chapman TJ,Smyth TR,Hillman SE,Eliseeva SI,Georas SN

Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis.
Molecular cell72(6)999-1012.e6(2018 12)
Yu Q,Qu K,Modis Y

ADAR1-mediated RNA editing is required for thymic self-tolerance and inhibition of autoimmunity.
EMBO reports19(12)(2018 12)
Nakahama T,Kato Y,Kim JI,Vongpipatana T,Suzuki Y,Walkley CR,Kawahara Y

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Ifih1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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