Eme1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eme1
Entrez Gene ID	268465
Full Name	essential meiotic structure-specific endonuclease 1
Synonyms	6820428D13
General protein information	Preferred Names essential meiotic structure-specific endonuclease 1 Names crossover junction endonuclease EME1 essential meiotic endonuclease 1 homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_030246037.1	XP_030101897.1	crossover junction endonuclease EME1 isoform X4
NM_177752.4	NP_808420.1	crossover junction endonuclease EME1
XM_006533408.2	XP_006533471.1	crossover junction endonuclease EME1 isoform X1
XM_006533408.3	XP_006533471.1	crossover junction endonuclease EME1 isoform X1
XM_011249045.1	XP_011247347.1	crossover junction endonuclease EME1 isoform X2
NM_177752.5	NP_808420.1	crossover junction endonuclease EME1
XM_030246036.1	XP_030101896.1	crossover junction endonuclease EME1 isoform X3

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002302	XP_312668.4
Mus musculus (house mouse)	Eme1	NP_808420.1
Xenopus tropicalis (tropical clawed frog)	eme1	NP_001072299.1
Rattus norvegicus (Norway rat)	Eme1	NP_001099300.2
Gallus gallus (chicken)	EME1	XP_003642406.1
Danio rerio (zebrafish)	eme1	XP_005156276.1
Drosophila melanogaster (fruit fly)	mms4	NP_610611.1
Homo sapiens (human)	EME1	NP_689676.2
Pan troglodytes (chimpanzee)	EME1	XP_001169575.1
Canis lupus familiaris (dog)	EME1	XP_005624696.1

Gene Ontology
Bibliography

Related articles in PubMed

A mutation in the endonuclease domain of mouse MLH3 reveals novel roles for MutLγ during crossover formation in meiotic prophase I.
Toledo M, Sun X, Brieño-Enríquez MA, Raghavan V, Gray S, Pea J, Milano CR, Venkatesh A, Patel L, Borst PL, Alani E, Cohen PE
PLoS genetics15(6)e1008177(2019 06)

Gen1 and Eme1 Play Redundant Roles in DNA Repair and Meiotic Recombination in Mice.
Wang X, Wang H, Guo B, Zhang Y, Gong Y, Zhang C, Xu H, Wu X
DNA and cell biology35(10)585-590(2016 Oct)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

Cooperative control of holliday junction resolution and DNA repair by the SLX1 and MUS81-EME1 nucleases.
Castor D, Nair N, Déclais AC, Lachaud C, Toth R, Macartney TJ, Lilley DM, Arthur JS, Rouse J
Molecular cell52(2)221-33(2013 Oct)

Atypical E2F repressors and activators coordinate placental development.
Ouseph MM, Li J, Chen HZ, Pécot T, Wenzel P, Thompson JC, Comstock G, Chokshi V, Byrne M, Forde B, Chong JL, Huang K, Machiraju R, de Bruin A, Leone G
Developmental cell22(4)849-62(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eme1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eme1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu74316	XM_011249045.1 Latest version!	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu74317	XM_030246036.1 Latest version!	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu85799	XM_030246037.1 Latest version!	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu46362	XM_006533408.3 Latest version!	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu06778	NM_177752.5 Latest version!	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$335.30 $479.00
View Old Accession Versions >>
OMu46362	XM_006533408.2	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu06778	NM_177752.4	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$239.50-$335.30 $479.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eme1 ( NM_177752.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808420.1 Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), mRNA.

Price & Availability↑

CloneID	OMu06778
Clone ID Related Accession (Same CDS sequence)	NM_177752.4 , NM_177752.5
Accession Version	NM_177752.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495814400000
Organism	Mus musculus(house mouse)
Product	crossover junction endonuclease EME1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL645764.15. On May 2, 2007 this sequence version replaced NM_177752.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK078516.1, BC089459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGGATGGAG ATGACTGGAT GGAAGAGCCA ACAATCCTGG TGTTGGTCCT GGCAGAGGTC 
TTCATGTCCA TGGCCTACAA CTTAAAGCAG GCAAGTCCTA GCAGCACCGA GAAAGGGAAG 
GAGACCCTTC GGAGCTTTGT AACTGATGTC ACAGCAAAGA CGGGGAAAGC ATTGTCACTG 
GTGATTGTGG ACCAGGAGAA ATGCTTCAGG CCTCAGAATC CCCCGAGGAG AAGGAAATCA 
GGAATGGCAA ATAAACAGGC CAAAGCGAAG CATCAGCAGA GGCAAGAGTC TAGCACGGGA 
CTCATGGTGT CCAGGGCAGA CATGGAGAAG GCACTGGTGG ATCTGCAGCT TTATACAGAA 
GCCCAGGCGT GGATGGTGCA GAGCTGGAAG GAGCTGGCGG ACTTCACCTG TGCATTTACA 
AAGGCTGTGG CTGAAGCACC CTTCAAGAAG CTCCGGGATC AAGTCACCTT TTCCTTCTTC 
CTGGAGAAAG ACTGGGCTGG AGGGATGAAA GTGGACCAGT CCGGCAGGGG ACTGGCACTG 
ATCTGGAGGC GGCAGATCCA GCAGCTGAAC CGAGTCAGCT CGGAGATGGC CAGTGCTATT 
GTGGACGCCT ATCCCTCACC ACAGCTCCTG GTGCAGGCTT ATCAGCGGTG TTTCTCTGAG 
CAAGAACGTC AGAATTTGCT GGCTGACATA CAAGTGCGAC GTGGGGAAGG TGTGACAGCC 
ACCTCCCGCC GTGTTGGGCC AGAATTATCC AGGCGGATCT ACCTTCAAAT GACCACAGCG 
CAACCAGATC TCATCTTAGA CAGTGTTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808420.1
CDS	89..1801
Translation	MALRRLSLSRLSTESDSEDLPTFAFLKKEPSSTNRKPPQRAKNIVVVTSDSEASCPPSPGLKGPPCVPSAAGAPPQAGPVRVLSSSSEDEDVFVPLAERITCKLLTSKQLCPELSSSSLKTGLDGQNNASAPCDWKRQPWPKIPDVPLHGALEKSAANDEDSLLDDQCRQLPTYQATCRELAVSKTNSDRPLPKKRTKHIQTVQSGGSQGCWRPGQASRKENTPRQHERKKKAEMIKRLKAQRPEECLKHIVVVLDPVLLQMEGGGQLLGALQAMECSCVIEVQAIPRSITWRRRRTELVEDGDDWMEEPTILVLVLAEVFMSMAYNLKQASPSSTEKGKETLRSFVTDVTAKTGKALSLVIVDQEKCFRPQNPPRRRKSGMANKQAKAKHQQRQESSTGLMVSRADMEKALVDLQLYTEAQAWMVQSWKELADFTCAFTKAVAEAPFKKLRDQVTFSFFLEKDWAGGMKVDQSGRGLALIWRRQIQQLNRVSSEMASAIVDAYPSPQLLVQAYQRCFSEQERQNLLADIQVRRGEGVTATSRRVGPELSRRIYLQMTTAQPDLILDSVD

Target ORF information:

RefSeq Version	NM_177752.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177752.4

ORF Insert Sequence:

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGGATGGAG ATGACTGGAT GGAAGAGCCA ACAATCCTGG TGTTGGTCCT GGCAGAGGTC 
TTCATGTCCA TGGCCTACAA CTTAAAGCAG GCAAGTCCTA GCAGCACCGA GAAAGGGAAG 
GAGACCCTTC GGAGCTTTGT AACTGATGTC ACAGCAAAGA CGGGGAAAGC ATTGTCACTG 
GTGATTGTGG ACCAGGAGAA ATGCTTCAGG CCTCAGAATC CCCCGAGGAG AAGGAAATCA 
GGAATGGCAA ATAAACAGGC CAAAGCGAAG CATCAGCAGA GGCAAGAGTC TAGCACGGGA 
CTCATGGTGT CCAGGGCAGA CATGGAGAAG GCACTGGTGG ATCTGCAGCT TTATACAGAA 
GCCCAGGCGT GGATGGTGCA GAGCTGGAAG GAGCTGGCGG ACTTCACCTG TGCATTTACA 
AAGGCTGTGG CTGAAGCACC CTTCAAGAAG CTCCGGGATC AAGTCACCTT TTCCTTCTTC 
CTGGAGAAAG ACTGGGCTGG AGGGATGAAA GTGGACCAGT CCGGCAGGGG ACTGGCACTG 
ATCTGGAGGC GGCAGATCCA GCAGCTGAAC CGAGTCAGCT CGGAGATGGC CAGTGCTATT 
GTGGACGCCT ATCCCTCACC ACAGCTCCTG GTGCAGGCTT ATCAGCGGTG TTTCTCTGAG 
CAAGAACGTC AGAATTTGCT GGCTGACATA CAAGTGCGAC GTGGGGAAGG TGTGACAGCC 
ACCTCCCGCC GTGTTGGGCC AGAATTATCC AGGCGGATCT ACCTTCAAAT GACCACAGCG 
CAACCAGATC TCATCTTAGA CAGTGTTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eme1 ( XM_011249045.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247347.1 Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu74316
Clone ID Related Accession (Same CDS sequence)	XM_011249045.1
Accession Version	XM_011249045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	crossover junction endonuclease EME1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGTG GGGGCCAGCT CCTGGGAGCG CTGCAGGCCA TGGAGTGCAG CTGTGTGATT 
GAAGTCCAGG CCATACCTCG AAGCATCACT TGGAGGAGGA GGAGGACAGA GCTGGTAGAG 
TTGCCTGTTG CAGAGAGAGG AGCAACTGGT AGCATCCCTT TCCCCTGCCT GAATGAGGAT 
GGAGATGACT GGATGGAAGA GCCAACAATC CTGGTGTTGG TCCTGGCAGA GGTCTTCATG 
TCCATGGCCT ACAACTTAAA GCAGGCAAGT CCTAGCAGCA CCGAGAAAGG GAAGGAGACC 
CTTCGGAGCT TTGTAACTGA TGTCACAGCA AAGACGGGGA AAGCATTGTC ACTGGTGATT 
GTGGACCAGG AGAAATGCTT CAGGCCTCAG AATCCCCCGA GGAGAAGGAA ATCAGGAATG 
GCAAATAAAC AGGCCAAAGC GAAGCATCAG CAGAGGCAAG AGTCTAGCAC GGGACTCATG 
GTGTCCAGGG CAGACATGGA GAAGGCACTG GTGGATCTGC AGCTTTATAC AGAAGCCCAG 
GCGTGGATGG TGCAGAGCTG GAAGGAGCTG GCGGACTTCA CCTGTGCATT TACAAAGGCT 
GTGGCTGAAG CACCCTTCAA GAAGCTCCGG GATCAAGTCA CCTTTTCCTT CTTCCTGGAG 
AAAGACTGGG CTGGAGGGAT GAAAGTGGAC CAGTCCGGCA GGGGACTGGC ACTGATCTGG 
AGGCGGCAGA TCCAGCAGCT GAACCGAGTC AGCTCGGAGA TGGCCAGTGC TATTGTGGAC 
GCCTATCCCT CACCACAGCT CCTGGTGCAG GCTTATCAGC GGTGTTTCTC TGAGCAAGAA 
CGTCAGAATT TGCTGGCTGA CATACAAGTG CGACGTGGGG AAGGTGTGAC AGCCACCTCC 
CGCCGTGTTG GGCCAGAATT ATCCAGGCGG ATCTACCTTC AAATGACCAC AGCGCAACCA 
GATCTCATCT TAGACAGTGT TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247347.1
CDS	102..1088
Translation	MEGGGQLLGALQAMECSCVIEVQAIPRSITWRRRRTELVELPVAERGATGSIPFPCLNEDGDDWMEEPTILVLVLAEVFMSMAYNLKQASPSSTEKGKETLRSFVTDVTAKTGKALSLVIVDQEKCFRPQNPPRRRKSGMANKQAKAKHQQRQESSTGLMVSRADMEKALVDLQLYTEAQAWMVQSWKELADFTCAFTKAVAEAPFKKLRDQVTFSFFLEKDWAGGMKVDQSGRGLALIWRRQIQQLNRVSSEMASAIVDAYPSPQLLVQAYQRCFSEQERQNLLADIQVRRGEGVTATSRRVGPELSRRIYLQMTTAQPDLILDSVD

Target ORF information:

RefSeq Version	XM_011249045.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249045.1

ORF Insert Sequence:

ATGGAAGGTG GGGGCCAGCT CCTGGGAGCG CTGCAGGCCA TGGAGTGCAG CTGTGTGATT 
GAAGTCCAGG CCATACCTCG AAGCATCACT TGGAGGAGGA GGAGGACAGA GCTGGTAGAG 
TTGCCTGTTG CAGAGAGAGG AGCAACTGGT AGCATCCCTT TCCCCTGCCT GAATGAGGAT 
GGAGATGACT GGATGGAAGA GCCAACAATC CTGGTGTTGG TCCTGGCAGA GGTCTTCATG 
TCCATGGCCT ACAACTTAAA GCAGGCAAGT CCTAGCAGCA CCGAGAAAGG GAAGGAGACC 
CTTCGGAGCT TTGTAACTGA TGTCACAGCA AAGACGGGGA AAGCATTGTC ACTGGTGATT 
GTGGACCAGG AGAAATGCTT CAGGCCTCAG AATCCCCCGA GGAGAAGGAA ATCAGGAATG 
GCAAATAAAC AGGCCAAAGC GAAGCATCAG CAGAGGCAAG AGTCTAGCAC GGGACTCATG 
GTGTCCAGGG CAGACATGGA GAAGGCACTG GTGGATCTGC AGCTTTATAC AGAAGCCCAG 
GCGTGGATGG TGCAGAGCTG GAAGGAGCTG GCGGACTTCA CCTGTGCATT TACAAAGGCT 
GTGGCTGAAG CACCCTTCAA GAAGCTCCGG GATCAAGTCA CCTTTTCCTT CTTCCTGGAG 
AAAGACTGGG CTGGAGGGAT GAAAGTGGAC CAGTCCGGCA GGGGACTGGC ACTGATCTGG 
AGGCGGCAGA TCCAGCAGCT GAACCGAGTC AGCTCGGAGA TGGCCAGTGC TATTGTGGAC 
GCCTATCCCT CACCACAGCT CCTGGTGCAG GCTTATCAGC GGTGTTTCTC TGAGCAAGAA 
CGTCAGAATT TGCTGGCTGA CATACAAGTG CGACGTGGGG AAGGTGTGAC AGCCACCTCC 
CGCCGTGTTG GGCCAGAATT ATCCAGGCGG ATCTACCTTC AAATGACCAC AGCGCAACCA 
GATCTCATCT TAGACAGTGT TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eme1 ( XM_030246036.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101896.1 Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu74317
Clone ID Related Accession (Same CDS sequence)	XM_030246036.1
Accession Version	XM_030246036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	crossover junction endonuclease EME1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGTTGCCTG TTGCAGAGAG AGGAGCAACT GGTAGCATCC CTTTCCCCTG CCTGAATGAG 
GTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101896.1
CDS	171..1139
Translation	MALRRLSLSRLSTESDSEDLPTFAFLKKEPSSTNRKPPQRAKNIVVVTSDSEASCPPSPGLKGPPCVPSAAGAPPQAGPVRVLSSSSEDEDVFVPLAERITCKLLTSKQLCPELSSSSLKTGLDGQNNASAPCDWKRQPWPKIPDVPLHGALEKSAANDEDSLLDDQCRQLPTYQATCRELAVSKTNSDRPLPKKRTKHIQTVQSGGSQGCWRPGQASRKENTPRQHERKKKAEMIKRLKAQRPEECLKHIVVVLDPVLLQMEGGGQLLGALQAMECSCVIEVQAIPRSITWRRRRTELVELPVAERGATGSIPFPCLNEVI

Target ORF information:

RefSeq Version	XM_030246036.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246036.1

ORF Insert Sequence:

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGTTGCCTG TTGCAGAGAG AGGAGCAACT GGTAGCATCC CTTTCCCCTG CCTGAATGAG 
GTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eme1 ( XM_030246037.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101897.1 Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X4, mRNA.

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CloneID	OMu85799
Clone ID Related Accession (Same CDS sequence)	XM_030246037.1
Accession Version	XM_030246037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	crossover junction endonuclease EME1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGTG GGGGCCAGCT CCTGGGAGCG CTGCAGGCCA TGGAGTGCAG CTGTGTGATT 
GAAGTCCAGG CCATACCTCG AAGCATCACT TGGAGGAGGA GGAGGACAGA GCTGGTAGAG 
GATGGAGATG ACTGGATGGA AGAGCCAACA ATCCTGGTGT TGGTCCTGGC AGAGGTCTTC 
ATGTCCATGG CCTACAACTT AAAGCAGGCA AGTCCTAGCA GCACCGAGAA AGGGAAGGAG 
ACCCTTCGGA GCTTTGTAAC TGATGTCACA GCAAAGACGG GGAAAGCATT GTCACTGGTG 
ATTGTGGACC AGGAGAAATG CTTCAGGCCT CAGAATCCCC CGAGGAGAAG GAAATCAGGA 
ATGGCAAATA AACAGGCCAA AGCGAAGCAT CAGCAGAGGC AAGAGTCTAG CACGGGACTC 
ATGGTGTCCA GGGCAGACAT GGAGAAGGCA CTGGTGGATC TGCAGCTTTA TACAGAAGCC 
CAGGCGTGGA TGGTGCAGAG CTGGAAGGAG CTGGCGGACT TCACCTGTGC ATTTACAAAG 
GCTGTGGCTG AAGCACCCTT CAAGAAGCTC CGGGATCAAG TCACCTTTTC CTTCTTCCTG 
GAGAAAGACT GGGCTGGAGG GATGAAAGTG GACCAGTCCG GCAGGGGACT GGCACTGATC 
TGGAGGCGGC AGATCCAGCA GCTGAACCGA GTCAGCTCGG AGATGGCCAG TGCTATTGTG 
GACGCCTATC CCTCACCACA GCTCCTGGTG CAGGCTTATC AGCGGTGTTT CTCTGAGCAA 
GAACGTCAGA ATTTGCTGGC TGACATACAA GTGCGACGTG GGGAAGGTGT GACAGCCACC 
TCCCGCCGTG TTGGGCCAGA ATTATCCAGG CGGATCTACC TTCAAATGAC CACAGCGCAA 
CCAGATCTCA TCTTAGACAG TGTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101897.1
CDS	153..1082
Translation	MEGGGQLLGALQAMECSCVIEVQAIPRSITWRRRRTELVEDGDDWMEEPTILVLVLAEVFMSMAYNLKQASPSSTEKGKETLRSFVTDVTAKTGKALSLVIVDQEKCFRPQNPPRRRKSGMANKQAKAKHQQRQESSTGLMVSRADMEKALVDLQLYTEAQAWMVQSWKELADFTCAFTKAVAEAPFKKLRDQVTFSFFLEKDWAGGMKVDQSGRGLALIWRRQIQQLNRVSSEMASAIVDAYPSPQLLVQAYQRCFSEQERQNLLADIQVRRGEGVTATSRRVGPELSRRIYLQMTTAQPDLILDSVD

Target ORF information:

RefSeq Version	XM_030246037.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246037.1

ORF Insert Sequence:

ATGGAAGGTG GGGGCCAGCT CCTGGGAGCG CTGCAGGCCA TGGAGTGCAG CTGTGTGATT 
GAAGTCCAGG CCATACCTCG AAGCATCACT TGGAGGAGGA GGAGGACAGA GCTGGTAGAG 
GATGGAGATG ACTGGATGGA AGAGCCAACA ATCCTGGTGT TGGTCCTGGC AGAGGTCTTC 
ATGTCCATGG CCTACAACTT AAAGCAGGCA AGTCCTAGCA GCACCGAGAA AGGGAAGGAG 
ACCCTTCGGA GCTTTGTAAC TGATGTCACA GCAAAGACGG GGAAAGCATT GTCACTGGTG 
ATTGTGGACC AGGAGAAATG CTTCAGGCCT CAGAATCCCC CGAGGAGAAG GAAATCAGGA 
ATGGCAAATA AACAGGCCAA AGCGAAGCAT CAGCAGAGGC AAGAGTCTAG CACGGGACTC 
ATGGTGTCCA GGGCAGACAT GGAGAAGGCA CTGGTGGATC TGCAGCTTTA TACAGAAGCC 
CAGGCGTGGA TGGTGCAGAG CTGGAAGGAG CTGGCGGACT TCACCTGTGC ATTTACAAAG 
GCTGTGGCTG AAGCACCCTT CAAGAAGCTC CGGGATCAAG TCACCTTTTC CTTCTTCCTG 
GAGAAAGACT GGGCTGGAGG GATGAAAGTG GACCAGTCCG GCAGGGGACT GGCACTGATC 
TGGAGGCGGC AGATCCAGCA GCTGAACCGA GTCAGCTCGG AGATGGCCAG TGCTATTGTG 
GACGCCTATC CCTCACCACA GCTCCTGGTG CAGGCTTATC AGCGGTGTTT CTCTGAGCAA 
GAACGTCAGA ATTTGCTGGC TGACATACAA GTGCGACGTG GGGAAGGTGT GACAGCCACC 
TCCCGCCGTG TTGGGCCAGA ATTATCCAGG CGGATCTACC TTCAAATGAC CACAGCGCAA 
CCAGATCTCA TCTTAGACAG TGTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eme1 ( XM_006533408.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533471.1 Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X1, mRNA.

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CloneID	OMu46362
Clone ID Related Accession (Same CDS sequence)	XM_006533408.2 , XM_006533408.3
Accession Version	XM_006533408.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	crossover junction endonuclease EME1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006533408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGTTGCCTG TTGCAGAGAG AGGAGCAACT GGTAGCATCC CTTTCCCCTG CCTGAATGAG 
GATGGAGATG ACTGGATGGA AGAGCCAACA ATCCTGGTGT TGGTCCTGGC AGAGGTCTTC 
ATGTCCATGG CCTACAACTT AAAGCAGGCA AGTCCTAGCA GCACCGAGAA AGGGAAGGAG 
ACCCTTCGGA GCTTTGTAAC TGATGTCACA GCAAAGACGG GGAAAGCATT GTCACTGGTG 
ATTGTGGACC AGGAGAAATG CTTCAGGCCT CAGAATCCCC CGAGGAGAAG GAAATCAGGA 
ATGGCAAATA AACAGGCCAA AGCGAAGCAT CAGCAGAGGC AAGAGTCTAG CACGGGACTC 
ATGGTGTCCA GGGCAGACAT GGAGAAGGCA CTGGTGGATC TGCAGCTTTA TACAGAAGCC 
CAGGCGTGGA TGGTGCAGAG CTGGAAGGAG CTGGCGGACT TCACCTGTGC ATTTACAAAG 
GCTGTGGCTG AAGCACCCTT CAAGAAGCTC CGGGATCAAG TCACCTTTTC CTTCTTCCTG 
GAGAAAGACT GGGCTGGAGG GATGAAAGTG GACCAGTCCG GCAGGGGACT GGCACTGATC 
TGGAGGCGGC AGATCCAGCA GCTGAACCGA GTCAGCTCGG AGATGGCCAG TGCTATTGTG 
GACGCCTATC CCTCACCACA GCTCCTGGTG CAGGCTTATC AGCGGTGTTT CTCTGAGCAA 
GAACGTCAGA ATTTGCTGGC TGACATACAA GTGCGACGTG GGGAAGGTGT GACAGCCACC 
TCCCGCCGTG TTGGGCCAGA ATTATCCAGG CGGATCTACC TTCAAATGAC CACAGCGCAA 
CCAGATCTCA TCTTAGACAG TGTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533471.1
CDS	118..1887
Translation	MALRRLSLSRLSTESDSEDLPTFAFLKKEPSSTNRKPPQRAKNIVVVTSDSEASCPPSPGLKGPPCVPSAAGAPPQAGPVRVLSSSSEDEDVFVPLAERITCKLLTSKQLCPELSSSSLKTGLDGQNNASAPCDWKRQPWPKIPDVPLHGALEKSAANDEDSLLDDQCRQLPTYQATCRELAVSKTNSDRPLPKKRTKHIQTVQSGGSQGCWRPGQASRKENTPRQHERKKKAEMIKRLKAQRPEECLKHIVVVLDPVLLQMEGGGQLLGALQAMECSCVIEVQAIPRSITWRRRRTELVELPVAERGATGSIPFPCLNEDGDDWMEEPTILVLVLAEVFMSMAYNLKQASPSSTEKGKETLRSFVTDVTAKTGKALSLVIVDQEKCFRPQNPPRRRKSGMANKQAKAKHQQRQESSTGLMVSRADMEKALVDLQLYTEAQAWMVQSWKELADFTCAFTKAVAEAPFKKLRDQVTFSFFLEKDWAGGMKVDQSGRGLALIWRRQIQQLNRVSSEMASAIVDAYPSPQLLVQAYQRCFSEQERQNLLADIQVRRGEGVTATSRRVGPELSRRIYLQMTTAQPDLILDSVD

Target ORF information:

RefSeq Version	XM_006533408.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533408.2

ORF Insert Sequence:

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGTTGCCTG TTGCAGAGAG AGGAGCAACT GGTAGCATCC CTTTCCCCTG CCTGAATGAG 
GATGGAGATG ACTGGATGGA AGAGCCAACA ATCCTGGTGT TGGTCCTGGC AGAGGTCTTC 
ATGTCCATGG CCTACAACTT AAAGCAGGCA AGTCCTAGCA GCACCGAGAA AGGGAAGGAG 
ACCCTTCGGA GCTTTGTAAC TGATGTCACA GCAAAGACGG GGAAAGCATT GTCACTGGTG 
ATTGTGGACC AGGAGAAATG CTTCAGGCCT CAGAATCCCC CGAGGAGAAG GAAATCAGGA 
ATGGCAAATA AACAGGCCAA AGCGAAGCAT CAGCAGAGGC AAGAGTCTAG CACGGGACTC 
ATGGTGTCCA GGGCAGACAT GGAGAAGGCA CTGGTGGATC TGCAGCTTTA TACAGAAGCC 
CAGGCGTGGA TGGTGCAGAG CTGGAAGGAG CTGGCGGACT TCACCTGTGC ATTTACAAAG 
GCTGTGGCTG AAGCACCCTT CAAGAAGCTC CGGGATCAAG TCACCTTTTC CTTCTTCCTG 
GAGAAAGACT GGGCTGGAGG GATGAAAGTG GACCAGTCCG GCAGGGGACT GGCACTGATC 
TGGAGGCGGC AGATCCAGCA GCTGAACCGA GTCAGCTCGG AGATGGCCAG TGCTATTGTG 
GACGCCTATC CCTCACCACA GCTCCTGGTG CAGGCTTATC AGCGGTGTTT CTCTGAGCAA 
GAACGTCAGA ATTTGCTGGC TGACATACAA GTGCGACGTG GGGAAGGTGT GACAGCCACC 
TCCCGCCGTG TTGGGCCAGA ATTATCCAGG CGGATCTACC TTCAAATGAC CACAGCGCAA 
CCAGATCTCA TCTTAGACAG TGTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eme1 ( XM_006533408.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533471.1 Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46362
Clone ID Related Accession (Same CDS sequence)	XM_006533408.2 , XM_006533408.3
Accession Version	XM_006533408.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	crossover junction endonuclease EME1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533408.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGTTGCCTG TTGCAGAGAG AGGAGCAACT GGTAGCATCC CTTTCCCCTG CCTGAATGAG 
GATGGAGATG ACTGGATGGA AGAGCCAACA ATCCTGGTGT TGGTCCTGGC AGAGGTCTTC 
ATGTCCATGG CCTACAACTT AAAGCAGGCA AGTCCTAGCA GCACCGAGAA AGGGAAGGAG 
ACCCTTCGGA GCTTTGTAAC TGATGTCACA GCAAAGACGG GGAAAGCATT GTCACTGGTG 
ATTGTGGACC AGGAGAAATG CTTCAGGCCT CAGAATCCCC CGAGGAGAAG GAAATCAGGA 
ATGGCAAATA AACAGGCCAA AGCGAAGCAT CAGCAGAGGC AAGAGTCTAG CACGGGACTC 
ATGGTGTCCA GGGCAGACAT GGAGAAGGCA CTGGTGGATC TGCAGCTTTA TACAGAAGCC 
CAGGCGTGGA TGGTGCAGAG CTGGAAGGAG CTGGCGGACT TCACCTGTGC ATTTACAAAG 
GCTGTGGCTG AAGCACCCTT CAAGAAGCTC CGGGATCAAG TCACCTTTTC CTTCTTCCTG 
GAGAAAGACT GGGCTGGAGG GATGAAAGTG GACCAGTCCG GCAGGGGACT GGCACTGATC 
TGGAGGCGGC AGATCCAGCA GCTGAACCGA GTCAGCTCGG AGATGGCCAG TGCTATTGTG 
GACGCCTATC CCTCACCACA GCTCCTGGTG CAGGCTTATC AGCGGTGTTT CTCTGAGCAA 
GAACGTCAGA ATTTGCTGGC TGACATACAA GTGCGACGTG GGGAAGGTGT GACAGCCACC 
TCCCGCCGTG TTGGGCCAGA ATTATCCAGG CGGATCTACC TTCAAATGAC CACAGCGCAA 
CCAGATCTCA TCTTAGACAG TGTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533471.1
CDS	171..1940
Translation	MALRRLSLSRLSTESDSEDLPTFAFLKKEPSSTNRKPPQRAKNIVVVTSDSEASCPPSPGLKGPPCVPSAAGAPPQAGPVRVLSSSSEDEDVFVPLAERITCKLLTSKQLCPELSSSSLKTGLDGQNNASAPCDWKRQPWPKIPDVPLHGALEKSAANDEDSLLDDQCRQLPTYQATCRELAVSKTNSDRPLPKKRTKHIQTVQSGGSQGCWRPGQASRKENTPRQHERKKKAEMIKRLKAQRPEECLKHIVVVLDPVLLQMEGGGQLLGALQAMECSCVIEVQAIPRSITWRRRRTELVELPVAERGATGSIPFPCLNEDGDDWMEEPTILVLVLAEVFMSMAYNLKQASPSSTEKGKETLRSFVTDVTAKTGKALSLVIVDQEKCFRPQNPPRRRKSGMANKQAKAKHQQRQESSTGLMVSRADMEKALVDLQLYTEAQAWMVQSWKELADFTCAFTKAVAEAPFKKLRDQVTFSFFLEKDWAGGMKVDQSGRGLALIWRRQIQQLNRVSSEMASAIVDAYPSPQLLVQAYQRCFSEQERQNLLADIQVRRGEGVTATSRRVGPELSRRIYLQMTTAQPDLILDSVD

Target ORF information:

RefSeq Version	XM_006533408.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533408.3

ORF Insert Sequence:

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGTTGCCTG TTGCAGAGAG AGGAGCAACT GGTAGCATCC CTTTCCCCTG CCTGAATGAG 
GATGGAGATG ACTGGATGGA AGAGCCAACA ATCCTGGTGT TGGTCCTGGC AGAGGTCTTC 
ATGTCCATGG CCTACAACTT AAAGCAGGCA AGTCCTAGCA GCACCGAGAA AGGGAAGGAG 
ACCCTTCGGA GCTTTGTAAC TGATGTCACA GCAAAGACGG GGAAAGCATT GTCACTGGTG 
ATTGTGGACC AGGAGAAATG CTTCAGGCCT CAGAATCCCC CGAGGAGAAG GAAATCAGGA 
ATGGCAAATA AACAGGCCAA AGCGAAGCAT CAGCAGAGGC AAGAGTCTAG CACGGGACTC 
ATGGTGTCCA GGGCAGACAT GGAGAAGGCA CTGGTGGATC TGCAGCTTTA TACAGAAGCC 
CAGGCGTGGA TGGTGCAGAG CTGGAAGGAG CTGGCGGACT TCACCTGTGC ATTTACAAAG 
GCTGTGGCTG AAGCACCCTT CAAGAAGCTC CGGGATCAAG TCACCTTTTC CTTCTTCCTG 
GAGAAAGACT GGGCTGGAGG GATGAAAGTG GACCAGTCCG GCAGGGGACT GGCACTGATC 
TGGAGGCGGC AGATCCAGCA GCTGAACCGA GTCAGCTCGG AGATGGCCAG TGCTATTGTG 
GACGCCTATC CCTCACCACA GCTCCTGGTG CAGGCTTATC AGCGGTGTTT CTCTGAGCAA 
GAACGTCAGA ATTTGCTGGC TGACATACAA GTGCGACGTG GGGAAGGTGT GACAGCCACC 
TCCCGCCGTG TTGGGCCAGA ATTATCCAGG CGGATCTACC TTCAAATGAC CACAGCGCAA 
CCAGATCTCA TCTTAGACAG TGTTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eme1 ( NM_177752.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808420.1 Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), mRNA.

Price & Availability↑

CloneID	OMu06778
Clone ID Related Accession (Same CDS sequence)	NM_177752.4 , NM_177752.5
Accession Version	NM_177752.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573315200000
Organism	Mus musculus(house mouse)
Product	crossover junction endonuclease EME1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645764.15. On Jul 6, 2018 this sequence version replaced NM_177752.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK078516.1, BC089459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGGATGGAG ATGACTGGAT GGAAGAGCCA ACAATCCTGG TGTTGGTCCT GGCAGAGGTC 
TTCATGTCCA TGGCCTACAA CTTAAAGCAG GCAAGTCCTA GCAGCACCGA GAAAGGGAAG 
GAGACCCTTC GGAGCTTTGT AACTGATGTC ACAGCAAAGA CGGGGAAAGC ATTGTCACTG 
GTGATTGTGG ACCAGGAGAA ATGCTTCAGG CCTCAGAATC CCCCGAGGAG AAGGAAATCA 
GGAATGGCAA ATAAACAGGC CAAAGCGAAG CATCAGCAGA GGCAAGAGTC TAGCACGGGA 
CTCATGGTGT CCAGGGCAGA CATGGAGAAG GCACTGGTGG ATCTGCAGCT TTATACAGAA 
GCCCAGGCGT GGATGGTGCA GAGCTGGAAG GAGCTGGCGG ACTTCACCTG TGCATTTACA 
AAGGCTGTGG CTGAAGCACC CTTCAAGAAG CTCCGGGATC AAGTCACCTT TTCCTTCTTC 
CTGGAGAAAG ACTGGGCTGG AGGGATGAAA GTGGACCAGT CCGGCAGGGG ACTGGCACTG 
ATCTGGAGGC GGCAGATCCA GCAGCTGAAC CGAGTCAGCT CGGAGATGGC CAGTGCTATT 
GTGGACGCCT ATCCCTCACC ACAGCTCCTG GTGCAGGCTT ATCAGCGGTG TTTCTCTGAG 
CAAGAACGTC AGAATTTGCT GGCTGACATA CAAGTGCGAC GTGGGGAAGG TGTGACAGCC 
ACCTCCCGCC GTGTTGGGCC AGAATTATCC AGGCGGATCT ACCTTCAAAT GACCACAGCG 
CAACCAGATC TCATCTTAGA CAGTGTTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808420.1
CDS	78..1790
Translation	MALRRLSLSRLSTESDSEDLPTFAFLKKEPSSTNRKPPQRAKNIVVVTSDSEASCPPSPGLKGPPCVPSAAGAPPQAGPVRVLSSSSEDEDVFVPLAERITCKLLTSKQLCPELSSSSLKTGLDGQNNASAPCDWKRQPWPKIPDVPLHGALEKSAANDEDSLLDDQCRQLPTYQATCRELAVSKTNSDRPLPKKRTKHIQTVQSGGSQGCWRPGQASRKENTPRQHERKKKAEMIKRLKAQRPEECLKHIVVVLDPVLLQMEGGGQLLGALQAMECSCVIEVQAIPRSITWRRRRTELVEDGDDWMEEPTILVLVLAEVFMSMAYNLKQASPSSTEKGKETLRSFVTDVTAKTGKALSLVIVDQEKCFRPQNPPRRRKSGMANKQAKAKHQQRQESSTGLMVSRADMEKALVDLQLYTEAQAWMVQSWKELADFTCAFTKAVAEAPFKKLRDQVTFSFFLEKDWAGGMKVDQSGRGLALIWRRQIQQLNRVSSEMASAIVDAYPSPQLLVQAYQRCFSEQERQNLLADIQVRRGEGVTATSRRVGPELSRRIYLQMTTAQPDLILDSVD

Target ORF information:

RefSeq Version	NM_177752.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus essential meiotic structure-specific endonuclease 1 (Eme1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177752.5

ORF Insert Sequence:

ATGGCTCTAA GAAGGTTATC CCTGTCTCGC CTCTCCACGG AGAGTGATTC TGAGGACCTG 
CCCACATTTG CCTTTTTGAA GAAGGAACCA TCTTCAACCA ACAGGAAGCC ACCTCAGAGG 
GCGAAGAACA TAGTGGTTGT CACCTCAGAT TCTGAAGCCT CCTGTCCTCC ATCACCAGGC 
CTCAAAGGTC CACCATGTGT CCCCAGTGCA GCTGGAGCTC CCCCACAAGC AGGGCCAGTC 
AGAGTGCTAA GCAGTAGCAG TGAGGACGAA GATGTATTTG TCCCCCTAGC TGAGAGGATC 
ACATGTAAGC TTTTGACGAG CAAGCAGCTG TGCCCTGAGC TCTCTAGCTC CTCACTTAAA 
ACAGGTTTGG ATGGCCAAAA TAATGCCAGC GCACCATGTG ACTGGAAAAG GCAACCATGG 
CCAAAGATTC CCGATGTTCC CCTCCACGGT GCCTTAGAGA AGAGTGCTGC AAATGATGAG 
GACTCTCTCT TAGACGATCA GTGTCGTCAG CTTCCAACCT ACCAGGCTAC CTGCAGGGAG 
CTGGCAGTCT CCAAAACAAA TTCCGACCGA CCTCTACCCA AGAAGAGAAC CAAGCATATT 
CAGACGGTCC AGAGCGGAGG CTCTCAGGGA TGCTGGCGAC CGGGACAGGC AAGCAGGAAG 
GAAAACACCC CGAGGCAGCA TGAAAGAAAA AAGAAGGCAG AGATGATCAA GAGGCTCAAA 
GCCCAGAGGC CAGAGGAATG CCTGAAGCAC ATCGTGGTGG TGCTGGATCC AGTGCTTTTA 
CAGATGGAAG GTGGGGGCCA GCTCCTGGGA GCGCTGCAGG CCATGGAGTG CAGCTGTGTG 
ATTGAAGTCC AGGCCATACC TCGAAGCATC ACTTGGAGGA GGAGGAGGAC AGAGCTGGTA 
GAGGATGGAG ATGACTGGAT GGAAGAGCCA ACAATCCTGG TGTTGGTCCT GGCAGAGGTC 
TTCATGTCCA TGGCCTACAA CTTAAAGCAG GCAAGTCCTA GCAGCACCGA GAAAGGGAAG 
GAGACCCTTC GGAGCTTTGT AACTGATGTC ACAGCAAAGA CGGGGAAAGC ATTGTCACTG 
GTGATTGTGG ACCAGGAGAA ATGCTTCAGG CCTCAGAATC CCCCGAGGAG AAGGAAATCA 
GGAATGGCAA ATAAACAGGC CAAAGCGAAG CATCAGCAGA GGCAAGAGTC TAGCACGGGA 
CTCATGGTGT CCAGGGCAGA CATGGAGAAG GCACTGGTGG ATCTGCAGCT TTATACAGAA 
GCCCAGGCGT GGATGGTGCA GAGCTGGAAG GAGCTGGCGG ACTTCACCTG TGCATTTACA 
AAGGCTGTGG CTGAAGCACC CTTCAAGAAG CTCCGGGATC AAGTCACCTT TTCCTTCTTC 
CTGGAGAAAG ACTGGGCTGG AGGGATGAAA GTGGACCAGT CCGGCAGGGG ACTGGCACTG 
ATCTGGAGGC GGCAGATCCA GCAGCTGAAC CGAGTCAGCT CGGAGATGGC CAGTGCTATT 
GTGGACGCCT ATCCCTCACC ACAGCTCCTG GTGCAGGCTT ATCAGCGGTG TTTCTCTGAG 
CAAGAACGTC AGAATTTGCT GGCTGACATA CAAGTGCGAC GTGGGGAAGG TGTGACAGCC 
ACCTCCCGCC GTGTTGGGCC AGAATTATCC AGGCGGATCT ACCTTCAAAT GACCACAGCG 
CAACCAGATC TCATCTTAGA CAGTGTTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mutation in the endonuclease domain of mouse MLH3 reveals novel roles for MutLγ during crossover formation in meiotic prophase I.
PLoS genetics15(6)e1008177(2019 06)
Toledo M,Sun X,Brieño-Enríquez MA,Raghavan V,Gray S,Pea J,Milano CR,Venkatesh A,Patel L,Borst PL,Alani E,Cohen PE

Gen1 and Eme1 Play Redundant Roles in DNA Repair and Meiotic Recombination in Mice.
DNA and cell biology35(10)585-590(2016 Oct)
Wang X,Wang H,Guo B,Zhang Y,Gong Y,Zhang C,Xu H,Wu X

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Cooperative control of holliday junction resolution and DNA repair by the SLX1 and MUS81-EME1 nucleases.
Molecular cell52(2)221-33(2013 Oct)
Castor D,Nair N,Déclais AC,Lachaud C,Toth R,Macartney TJ,Lilley DM,Arthur JS,Rouse J

Atypical E2F repressors and activators coordinate placental development.
Developmental cell22(4)849-62(2012 Apr)
Ouseph MM,Li J,Chen HZ,Pécot T,Wenzel P,Thompson JC,Comstock G,Chokshi V,Byrne M,Forde B,Chong JL,Huang K,Machiraju R,de Bruin A,Leone G

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Eme1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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