EME1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EME1
Entrez Gene ID	146956
Full Name	essential meiotic structure-specific endonuclease 1
Synonyms	MMS4L,SLX2A
General protein information	Preferred Names essential meiotic structure-specific endonuclease 1 Names crossover junction endonuclease EME1 MMS4 homolog SLX2 structure-specific endonuclease subunit homolog A essential meiotic endonuclease 1 homolog 1 essential meiotic endonuclease 1 homolog 2 hMMS4 homolog of yeast EME1 endonuclease
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.33
Summary	This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009].
Disorder MIM:	610885

mRNA and Protein(s)

mRNA	Protein	Name
XM_017024236.1	XP_016879725.1	crossover junction endonuclease EME1 isoform X1
NM_152463.2	NP_689676.2	crossover junction endonuclease EME1 isoform 2
XM_005257081.3	XP_005257138.1	crossover junction endonuclease EME1 isoform X2
NM_001166131.1	NP_001159603.1	crossover junction endonuclease EME1 isoform 1
XM_011524395.2	XP_011522697.1	crossover junction endonuclease EME1 isoform X3
NM_152463.3	NP_689676.2	crossover junction endonuclease EME1 isoform 2
XM_017024236.2	XP_016879725.1	crossover junction endonuclease EME1 isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002302	XP_312668.4
Mus musculus (house mouse)	Eme1	NP_808420.1
Xenopus tropicalis (tropical clawed frog)	eme1	NP_001072299.1
Rattus norvegicus (Norway rat)	Eme1	NP_001099300.2
Gallus gallus (chicken)	EME1	XP_003642406.1
Danio rerio (zebrafish)	eme1	XP_005156276.1
Drosophila melanogaster (fruit fly)	mms4	NP_610611.1
Homo sapiens (human)	EME1	NP_689676.2
Pan troglodytes (chimpanzee)	EME1	XP_001169575.1
Canis lupus familiaris (dog)	EME1	XP_005624696.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphorylation by CK2 regulates MUS81/EME1 in mitosis and after replication stress.
Palma A, Pugliese GM, Murfuni I, Marabitti V, Malacaria E, Rinalducci S, Minoprio A, Sanchez M, Mazzei F, Zolla L, Franchitto A, Pichierri P
Nucleic acids research46(10)5109-5124(2018 06)

Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage.
Saugar I, Jiménez-Martín A, Tercero JA
Cell reports20(7)1553-1562(2017 08)

SLX4-SLX1 Protein-independent Down-regulation of MUS81-EME1 Protein by HIV-1 Viral Protein R (Vpr).
Zhou X, DeLucia M, Ahn J
The Journal of biological chemistry291(33)16936-16947(2016 08)

Crystal structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates.
Gwon GH, Jo A, Baek K, Jin KS, Fu Y, Lee JB, Kim Y, Cho Y
The EMBO journal33(9)1061-72(2014 May)

Coordinated actions of SLX1-SLX4 and MUS81-EME1 for Holliday junction resolution in human cells.
Wyatt HD, Sarbajna S, Matos J, West SC
Molecular cell52(2)234-47(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EME1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EME1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu50141	XM_005257081.3 Latest version!	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu76309	XM_011524395.2 Latest version!	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu12501	NM_152463.3 Latest version!	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu19201	NM_001166131.1 Latest version!	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu76307	XM_017024236.2 Latest version!	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu12501	NM_152463.2	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu76307	XM_017024236.1	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EME1 ( XM_005257081.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257138.1 Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu50141
Clone ID Related Accession (Same CDS sequence)	XM_005257081.3
Accession Version	XM_005257081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	crossover junction endonuclease EME1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATTGCTCT TACAGATGGA AGGTGGGGGC CAGCTCCTAG GAGCACTGCA GACCATGGAG 
TGCCGCTGTG TGATTGAGGC GCAGGCTGTG CCTTGCAGTG TCACTTGGAG GAGAAGGGCT 
GGGCCGTCTG AGGACAGAGA GGACTGGGTG GAGGAGCCAA CAGTACTGGT GTTGCTCCGG 
GCAGAGGCAT TTGTGTCCAT GATCGACAAT GGAAAGCAGG GAAGCCTGGA CAGCACTATG 
AAAGGGAAGG AAACGCTTCA GGGCTTTGTA ACTGACATCA CAGCAAAGAC AGCAGGGAAA 
GCTCTGTCAC TGGTGATTGT GGATCAGGAG AAATGCTTCA GTGCTCAGAA TCCTCCAAGA 
AGAGGGAAAC AGGGAGCAAA TAAACAGACC AAGAAGCAGC AGCAGAGACA ACCAGAGGCC 
AGCATAGGGT CCATGGTATC CAGGGTAGAC GCTGAAGAGG CATTGGTGGA TCTGCAGCTA 
CACACAGAAG CCCAGGCTCA AATTGTGCAG AGCTGGAAAG AGCTGGCCGA CTTCACATGC 
GCATTCACAA AGGCTGTGGC TGAGGCGCCC TTCAAGAAGC TCCGAGATGA AACTACCTTC 
TCCTTCTGTC TGGAGAGTGA CTGGGCTGGA GGGGTGAAGG TGGACCTTGC TGGCAGGGGA 
CTCGCACTAG TCTGGAGGAG ACAGATTCAG CAGCTGAACC GAGTCAGCCT GGAAATGGCC 
AGTGCAGTTG TGAATGCCTA TCCCTCCCCA CAGCTCCTGG TACAGGCTTA TCAGCAGTGT 
TTTTCGGATA AAGAACGCCA GAATTTGCTC GCAGACATAC AGGTGCGCCG TGGGGAAGGT 
GTGACATCCA CTTCTCGCCG CATTGGACCA GAACTATCCA GGCGTATCTA CCTTCAGATG 
ACCACTTTAC AGCCACATCT CTCTTTAGAT AGTGCTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257138.1
CDS	79..1320
Translation	MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTLLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

Target ORF information:

RefSeq Version	XM_005257081.3
Organism	Homo sapiens(human)
Definition	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257081.3

ORF Insert Sequence:

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATTGCTCT TACAGATGGA AGGTGGGGGC CAGCTCCTAG GAGCACTGCA GACCATGGAG 
TGCCGCTGTG TGATTGAGGC GCAGGCTGTG CCTTGCAGTG TCACTTGGAG GAGAAGGGCT 
GGGCCGTCTG AGGACAGAGA GGACTGGGTG GAGGAGCCAA CAGTACTGGT GTTGCTCCGG 
GCAGAGGCAT TTGTGTCCAT GATCGACAAT GGAAAGCAGG GAAGCCTGGA CAGCACTATG 
AAAGGGAAGG AAACGCTTCA GGGCTTTGTA ACTGACATCA CAGCAAAGAC AGCAGGGAAA 
GCTCTGTCAC TGGTGATTGT GGATCAGGAG AAATGCTTCA GTGCTCAGAA TCCTCCAAGA 
AGAGGGAAAC AGGGAGCAAA TAAACAGACC AAGAAGCAGC AGCAGAGACA ACCAGAGGCC 
AGCATAGGGT CCATGGTATC CAGGGTAGAC GCTGAAGAGG CATTGGTGGA TCTGCAGCTA 
CACACAGAAG CCCAGGCTCA AATTGTGCAG AGCTGGAAAG AGCTGGCCGA CTTCACATGC 
GCATTCACAA AGGCTGTGGC TGAGGCGCCC TTCAAGAAGC TCCGAGATGA AACTACCTTC 
TCCTTCTGTC TGGAGAGTGA CTGGGCTGGA GGGGTGAAGG TGGACCTTGC TGGCAGGGGA 
CTCGCACTAG TCTGGAGGAG ACAGATTCAG CAGCTGAACC GAGTCAGCCT GGAAATGGCC 
AGTGCAGTTG TGAATGCCTA TCCCTCCCCA CAGCTCCTGG TACAGGCTTA TCAGCAGTGT 
TTTTCGGATA AAGAACGCCA GAATTTGCTC GCAGACATAC AGGTGCGCCG TGGGGAAGGT 
GTGACATCCA CTTCTCGCCG CATTGGACCA GAACTATCCA GGCGTATCTA CCTTCAGATG 
ACCACTTTAC AGCCACATCT CTCTTTAGAT AGTGCTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( XM_011524395.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522697.1 Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu76309
Clone ID Related Accession (Same CDS sequence)	XM_011524395.2
Accession Version	XM_011524395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	crossover junction endonuclease EME1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGACA ATGGAAAGCA GGGAAGCCTG GACAGCACTA TGAAAGGGAA GGAAACGCTT 
CAGGGCTTTG TAACTGACAT CACAGCAAAG ACAGCAGGGA AAGCTCTGTC ACTGGTGATT 
GTGGATCAGG AGAAATGCTT CAGTGCTCAG AATCCTCCAA GAAGAGGGAA ACAGGGAGCA 
AATAAACAGA CCAAGAAGCA GCAGCAGAGA CAACCAGAGG CCAGCATAGG GTCCATGGTA 
TCCAGGGTAG ACGCTGAAGA GGCATTGGTG GATCTGCAGC TACACACAGA AGCCCAGGCT 
CAAATTGTGC AGAGCTGGAA AGAGCTGGCC GACTTCACAT GCGCATTCAC AAAGGCTGTG 
GCTGAGGCGC CCTTCAAGAA GCTCCGAGAT GAAACTACCT TCTCCTTCTG TCTGGAGAGT 
GACTGGGCTG GAGGGGTGAA GGTGGACCTT GCTGGCAGGG GACTCGCACT AGTCTGGAGG 
AGACAGATTC AGCAGCTGAA CCGAGTCAGC CTGGAAATGG CCAGTGCAGT TGTGAATGCC 
TATCCCTCCC CACAGCTCCT GGTACAGGCT TATCAGCAGT GTTTTTCGGA TAAAGAACGC 
CAGAATTTGC TCGCAGACAT ACAGGTGCGC CGTGGGGAAG GTGTGACATC CACTTCTCGC 
CGCATTGGAC CAGAACTATC CAGGCGTATC TACCTTCAGA TGACCACTTT ACAGCCACAT 
CTCTCTTTAG ATAGTGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522697.1
CDS	542..1285
Translation	MIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

Target ORF information:

RefSeq Version	XM_011524395.2
Organism	Homo sapiens(human)
Definition	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524395.2

ORF Insert Sequence:

ATGATCGACA ATGGAAAGCA GGGAAGCCTG GACAGCACTA TGAAAGGGAA GGAAACGCTT 
CAGGGCTTTG TAACTGACAT CACAGCAAAG ACAGCAGGGA AAGCTCTGTC ACTGGTGATT 
GTGGATCAGG AGAAATGCTT CAGTGCTCAG AATCCTCCAA GAAGAGGGAA ACAGGGAGCA 
AATAAACAGA CCAAGAAGCA GCAGCAGAGA CAACCAGAGG CCAGCATAGG GTCCATGGTA 
TCCAGGGTAG ACGCTGAAGA GGCATTGGTG GATCTGCAGC TACACACAGA AGCCCAGGCT 
CAAATTGTGC AGAGCTGGAA AGAGCTGGCC GACTTCACAT GCGCATTCAC AAAGGCTGTG 
GCTGAGGCGC CCTTCAAGAA GCTCCGAGAT GAAACTACCT TCTCCTTCTG TCTGGAGAGT 
GACTGGGCTG GAGGGGTGAA GGTGGACCTT GCTGGCAGGG GACTCGCACT AGTCTGGAGG 
AGACAGATTC AGCAGCTGAA CCGAGTCAGC CTGGAAATGG CCAGTGCAGT TGTGAATGCC 
TATCCCTCCC CACAGCTCCT GGTACAGGCT TATCAGCAGT GTTTTTCGGA TAAAGAACGC 
CAGAATTTGC TCGCAGACAT ACAGGTGCGC CGTGGGGAAG GTGTGACATC CACTTCTCGC 
CGCATTGGAC CAGAACTATC CAGGCGTATC TACCTTCAGA TGACCACTTT ACAGCCACAT 
CTCTCTTTAG ATAGTGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( NM_152463.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689676.2 Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12501
Clone ID Related Accession (Same CDS sequence)	NM_152463.2 , NM_152463.3
Accession Version	NM_152463.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529683200000
Organism	Homo sapiens(human)
Product	crossover junction endonuclease EME1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB245967.1, AK055926.1, AC004707.2 and BC016470.2. On Oct 8, 2009 this sequence version replaced NM_152463.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK055926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGACAGAG AGGACTGGGT GGAGGAGCCA ACAGTACTGG TGTTGCTCCG GGCAGAGGCA 
TTTGTGTCCA TGATCGACAA TGGAAAGCAG GGAAGCCTGG ACAGCACTAT GAAAGGGAAG 
GAAACGCTTC AGGGCTTTGT AACTGACATC ACAGCAAAGA CAGCAGGGAA AGCTCTGTCA 
CTGGTGATTG TGGATCAGGA GAAATGCTTC AGTGCTCAGA ATCCTCCAAG AAGAGGGAAA 
CAGGGAGCAA ATAAACAGAC CAAGAAGCAG CAGCAGAGAC AACCAGAGGC CAGCATAGGG 
TCCATGGTAT CCAGGGTAGA CGCTGAAGAG GCATTGGTGG ATCTGCAGCT ACACACAGAA 
GCCCAGGCTC AAATTGTGCA GAGCTGGAAA GAGCTGGCCG ACTTCACATG CGCATTCACA 
AAGGCTGTGG CTGAGGCGCC CTTCAAGAAG CTCCGAGATG AAACTACCTT CTCCTTCTGT 
CTGGAGAGTG ACTGGGCTGG AGGGGTGAAG GTGGACCTTG CTGGCAGGGG ACTCGCACTA 
GTCTGGAGGA GACAGATTCA GCAGCTGAAC CGAGTCAGCC TGGAAATGGC CAGTGCAGTT 
GTGAATGCCT ATCCCTCCCC ACAGCTCCTG GTACAGGCTT ATCAGCAGTG TTTTTCGGAT 
AAAGAACGCC AGAATTTGCT CGCAGACATA CAGGTGCGCC GTGGGGAAGG TGTGACATCC 
ACTTCTCGCC GCATTGGACC AGAACTATCC AGGCGTATCT ACCTTCAGAT GACCACTTTA 
CAGCCACATC TCTCTTTAGA TAGTGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689676.2
CDS	83..1795
Translation	MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

Target ORF information:

RefSeq Version	NM_152463.2
Organism	Homo sapiens(human)
Definition	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152463.2

ORF Insert Sequence:

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGACAGAG AGGACTGGGT GGAGGAGCCA ACAGTACTGG TGTTGCTCCG GGCAGAGGCA 
TTTGTGTCCA TGATCGACAA TGGAAAGCAG GGAAGCCTGG ACAGCACTAT GAAAGGGAAG 
GAAACGCTTC AGGGCTTTGT AACTGACATC ACAGCAAAGA CAGCAGGGAA AGCTCTGTCA 
CTGGTGATTG TGGATCAGGA GAAATGCTTC AGTGCTCAGA ATCCTCCAAG AAGAGGGAAA 
CAGGGAGCAA ATAAACAGAC CAAGAAGCAG CAGCAGAGAC AACCAGAGGC CAGCATAGGG 
TCCATGGTAT CCAGGGTAGA CGCTGAAGAG GCATTGGTGG ATCTGCAGCT ACACACAGAA 
GCCCAGGCTC AAATTGTGCA GAGCTGGAAA GAGCTGGCCG ACTTCACATG CGCATTCACA 
AAGGCTGTGG CTGAGGCGCC CTTCAAGAAG CTCCGAGATG AAACTACCTT CTCCTTCTGT 
CTGGAGAGTG ACTGGGCTGG AGGGGTGAAG GTGGACCTTG CTGGCAGGGG ACTCGCACTA 
GTCTGGAGGA GACAGATTCA GCAGCTGAAC CGAGTCAGCC TGGAAATGGC CAGTGCAGTT 
GTGAATGCCT ATCCCTCCCC ACAGCTCCTG GTACAGGCTT ATCAGCAGTG TTTTTCGGAT 
AAAGAACGCC AGAATTTGCT CGCAGACATA CAGGTGCGCC GTGGGGAAGG TGTGACATCC 
ACTTCTCGCC GCATTGGACC AGAACTATCC AGGCGTATCT ACCTTCAGAT GACCACTTTA 
CAGCCACATC TCTCTTTAGA TAGTGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( NM_152463.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689676.2 Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 2, mRNA.

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CloneID	OHu12501
Clone ID Related Accession (Same CDS sequence)	NM_152463.2 , NM_152463.3
Accession Version	NM_152463.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569686400000
Organism	Homo sapiens(human)
Product	crossover junction endonuclease EME1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK055926.1, AC004707.2 and BC016470.2. On Jun 2, 2019 this sequence version replaced NM_152463.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK055926.1, SRR6380200.115250.7 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGACAGAG AGGACTGGGT GGAGGAGCCA ACAGTACTGG TGTTGCTCCG GGCAGAGGCA 
TTTGTGTCCA TGATCGACAA TGGAAAGCAG GGAAGCCTGG ACAGCACTAT GAAAGGGAAG 
GAAACGCTTC AGGGCTTTGT AACTGACATC ACAGCAAAGA CAGCAGGGAA AGCTCTGTCA 
CTGGTGATTG TGGATCAGGA GAAATGCTTC AGTGCTCAGA ATCCTCCAAG AAGAGGGAAA 
CAGGGAGCAA ATAAACAGAC CAAGAAGCAG CAGCAGAGAC AACCAGAGGC CAGCATAGGG 
TCCATGGTAT CCAGGGTAGA CGCTGAAGAG GCATTGGTGG ATCTGCAGCT ACACACAGAA 
GCCCAGGCTC AAATTGTGCA GAGCTGGAAA GAGCTGGCCG ACTTCACATG CGCATTCACA 
AAGGCTGTGG CTGAGGCGCC CTTCAAGAAG CTCCGAGATG AAACTACCTT CTCCTTCTGT 
CTGGAGAGTG ACTGGGCTGG AGGGGTGAAG GTGGACCTTG CTGGCAGGGG ACTCGCACTA 
GTCTGGAGGA GACAGATTCA GCAGCTGAAC CGAGTCAGCC TGGAAATGGC CAGTGCAGTT 
GTGAATGCCT ATCCCTCCCC ACAGCTCCTG GTACAGGCTT ATCAGCAGTG TTTTTCGGAT 
AAAGAACGCC AGAATTTGCT CGCAGACATA CAGGTGCGCC GTGGGGAAGG TGTGACATCC 
ACTTCTCGCC GCATTGGACC AGAACTATCC AGGCGTATCT ACCTTCAGAT GACCACTTTA 
CAGCCACATC TCTCTTTAGA TAGTGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689676.2
CDS	74..1786
Translation	MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

Target ORF information:

RefSeq Version	NM_152463.3
Organism	Homo sapiens(human)
Definition	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152463.3

ORF Insert Sequence:

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGACAGAG AGGACTGGGT GGAGGAGCCA ACAGTACTGG TGTTGCTCCG GGCAGAGGCA 
TTTGTGTCCA TGATCGACAA TGGAAAGCAG GGAAGCCTGG ACAGCACTAT GAAAGGGAAG 
GAAACGCTTC AGGGCTTTGT AACTGACATC ACAGCAAAGA CAGCAGGGAA AGCTCTGTCA 
CTGGTGATTG TGGATCAGGA GAAATGCTTC AGTGCTCAGA ATCCTCCAAG AAGAGGGAAA 
CAGGGAGCAA ATAAACAGAC CAAGAAGCAG CAGCAGAGAC AACCAGAGGC CAGCATAGGG 
TCCATGGTAT CCAGGGTAGA CGCTGAAGAG GCATTGGTGG ATCTGCAGCT ACACACAGAA 
GCCCAGGCTC AAATTGTGCA GAGCTGGAAA GAGCTGGCCG ACTTCACATG CGCATTCACA 
AAGGCTGTGG CTGAGGCGCC CTTCAAGAAG CTCCGAGATG AAACTACCTT CTCCTTCTGT 
CTGGAGAGTG ACTGGGCTGG AGGGGTGAAG GTGGACCTTG CTGGCAGGGG ACTCGCACTA 
GTCTGGAGGA GACAGATTCA GCAGCTGAAC CGAGTCAGCC TGGAAATGGC CAGTGCAGTT 
GTGAATGCCT ATCCCTCCCC ACAGCTCCTG GTACAGGCTT ATCAGCAGTG TTTTTCGGAT 
AAAGAACGCC AGAATTTGCT CGCAGACATA CAGGTGCGCC GTGGGGAAGG TGTGACATCC 
ACTTCTCGCC GCATTGGACC AGAACTATCC AGGCGTATCT ACCTTCAGAT GACCACTTTA 
CAGCCACATC TCTCTTTAGA TAGTGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( NM_001166131.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159603.1 Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 1, mRNA.

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CloneID	OHu19201
Clone ID Related Accession (Same CDS sequence)	NM_001166131.1
Accession Version	NM_001166131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Homo sapiens(human)
Product	crossover junction endonuclease EME1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB245967.1, DA006645.1 and BC016470.2. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC016470.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2154529, SAMEA2163105 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGACAGAG AGGACTGGGT GGAGGAGCCA ACAGTACTGG TGTTGCTCCG GGCAGAGGCA 
TTTGTGTCCA TGATCGACAA TGGAAAGCAG GGAAGCCTGG ACAGCACTAT GAAAGGGAAG 
GAAACGCTTC AGGGCTTTGT AACTGACATC ACAGCAAAGA CAGCAGGGAA AGCTCTGTCA 
CTGGTGATTG TGGATCAGGA GAAATGCTTC AGCCTGGAGC TGCTGTTCTT TGATTTCCTC 
CCCTGCACCA GTGCTCAGAA TCCTCCAAGA AGAGGGAAAC AGGGAGCAAA TAAACAGACC 
AAGAAGCAGC AGCAGAGACA ACCAGAGGCC AGCATAGGGT CCATGGTATC CAGGGTAGAC 
GCTGAAGAGG CATTGGTGGA TCTGCAGCTA CACACAGAAG CCCAGGCTCA AATTGTGCAG 
AGCTGGAAAG AGCTGGCCGA CTTCACATGC GCATTCACAA AGGCTGTGGC TGAGGCGCCC 
TTCAAGAAGC TCCGAGATGA AACTACCTTC TCCTTCTGTC TGGAGAGTGA CTGGGCTGGA 
GGGGTGAAGG TGGACCTTGC TGGCAGGGGA CTCGCACTAG TCTGGAGGAG ACAGATTCAG 
CAGCTGAACC GAGTCAGCCT GGAAATGGCC AGTGCAGTTG TGAATGCCTA TCCCTCCCCA 
CAGCTCCTGG TACAGGCTTA TCAGCAGTGT TTTTCGGATA AAGAACGCCA GAATTTGCTC 
GCAGACATAC AGGTGCGCCG TGGGGAAGGT GTGACATCCA CTTCTCGCCG CATTGGACCA 
GAACTATCCA GGCGTATCTA CCTTCAGATG ACCACTTTAC AGCCACATCT CTCTTTAGAT 
AGTGCTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159603.1
CDS	83..1834
Translation	MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSLELLFFDFLPCTSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

Target ORF information:

RefSeq Version	NM_001166131.1
Organism	Homo sapiens(human)
Definition	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166131.1

ORF Insert Sequence:

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGACAGAG AGGACTGGGT GGAGGAGCCA ACAGTACTGG TGTTGCTCCG GGCAGAGGCA 
TTTGTGTCCA TGATCGACAA TGGAAAGCAG GGAAGCCTGG ACAGCACTAT GAAAGGGAAG 
GAAACGCTTC AGGGCTTTGT AACTGACATC ACAGCAAAGA CAGCAGGGAA AGCTCTGTCA 
CTGGTGATTG TGGATCAGGA GAAATGCTTC AGCCTGGAGC TGCTGTTCTT TGATTTCCTC 
CCCTGCACCA GTGCTCAGAA TCCTCCAAGA AGAGGGAAAC AGGGAGCAAA TAAACAGACC 
AAGAAGCAGC AGCAGAGACA ACCAGAGGCC AGCATAGGGT CCATGGTATC CAGGGTAGAC 
GCTGAAGAGG CATTGGTGGA TCTGCAGCTA CACACAGAAG CCCAGGCTCA AATTGTGCAG 
AGCTGGAAAG AGCTGGCCGA CTTCACATGC GCATTCACAA AGGCTGTGGC TGAGGCGCCC 
TTCAAGAAGC TCCGAGATGA AACTACCTTC TCCTTCTGTC TGGAGAGTGA CTGGGCTGGA 
GGGGTGAAGG TGGACCTTGC TGGCAGGGGA CTCGCACTAG TCTGGAGGAG ACAGATTCAG 
CAGCTGAACC GAGTCAGCCT GGAAATGGCC AGTGCAGTTG TGAATGCCTA TCCCTCCCCA 
CAGCTCCTGG TACAGGCTTA TCAGCAGTGT TTTTCGGATA AAGAACGCCA GAATTTGCTC 
GCAGACATAC AGGTGCGCCG TGGGGAAGGT GTGACATCCA CTTCTCGCCG CATTGGACCA 
GAACTATCCA GGCGTATCTA CCTTCAGATG ACCACTTTAC AGCCACATCT CTCTTTAGAT 
AGTGCTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( XM_017024236.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879725.1 Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X1, mRNA.

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CloneID	OHu76307
Clone ID Related Accession (Same CDS sequence)	XM_017024236.1 , XM_017024236.2
Accession Version	XM_017024236.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	crossover junction endonuclease EME1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGGAAGCC TGGACAGCAC TATGAAAGGG AAGGAAACGC TTCAGGGCTT TGTAACTGAC 
ATCACAGCAA AGACAGCAGG GAAAGCTCTG TCACTGGTGA TTGTGGATCA GGAGAAATGC 
TTCAGTGCTC AGAATCCTCC AAGAAGAGGG AAACAGGGAG CAAATAAACA GACCAAGAAG 
CAGCAGCAGA GACAACCAGA GGCCAGCATA GGGTCCATGG TATCCAGGGT AGACGCTGAA 
GAGGCATTGG TGGATCTGCA GCTACACACA GAAGCCCAGG CTCAAATTGT GCAGAGCTGG 
AAAGAGCTGG CCGACTTCAC ATGCGCATTC ACAAAGGCTG TGGCTGAGGC GCCCTTCAAG 
AAGCTCCGAG ATGAAACTAC CTTCTCCTTC TGTCTGGAGA GTGACTGGGC TGGAGGGGTG 
AAGGTGGACC TTGCTGGCAG GGGACTCGCA CTAGTCTGGA GGAGACAGAT TCAGCAGCTG 
AACCGAGTCA GCCTGGAAAT GGCCAGTGCA GTTGTGAATG CCTATCCCTC CCCACAGCTC 
CTGGTACAGG CTTATCAGCA GTGTTTTTCG GATAAAGAAC GCCAGAATTT GCTCGCAGAC 
ATACAGGTGC GCCGTGGGGA AGGTGTGACA TCCACTTCTC GCCGCATTGG ACCAGAACTA 
TCCAGGCGTA TCTACCTTCA GATGACCACT TTACAGCCAC ATCTCTCTTT AGATAGTGCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879725.1
CDS	83..1708
Translation	MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

Target ORF information:

RefSeq Version	XM_017024236.1
Organism	Homo sapiens(human)
Definition	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024236.1

ORF Insert Sequence:

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGGAAGCC TGGACAGCAC TATGAAAGGG AAGGAAACGC TTCAGGGCTT TGTAACTGAC 
ATCACAGCAA AGACAGCAGG GAAAGCTCTG TCACTGGTGA TTGTGGATCA GGAGAAATGC 
TTCAGTGCTC AGAATCCTCC AAGAAGAGGG AAACAGGGAG CAAATAAACA GACCAAGAAG 
CAGCAGCAGA GACAACCAGA GGCCAGCATA GGGTCCATGG TATCCAGGGT AGACGCTGAA 
GAGGCATTGG TGGATCTGCA GCTACACACA GAAGCCCAGG CTCAAATTGT GCAGAGCTGG 
AAAGAGCTGG CCGACTTCAC ATGCGCATTC ACAAAGGCTG TGGCTGAGGC GCCCTTCAAG 
AAGCTCCGAG ATGAAACTAC CTTCTCCTTC TGTCTGGAGA GTGACTGGGC TGGAGGGGTG 
AAGGTGGACC TTGCTGGCAG GGGACTCGCA CTAGTCTGGA GGAGACAGAT TCAGCAGCTG 
AACCGAGTCA GCCTGGAAAT GGCCAGTGCA GTTGTGAATG CCTATCCCTC CCCACAGCTC 
CTGGTACAGG CTTATCAGCA GTGTTTTTCG GATAAAGAAC GCCAGAATTT GCTCGCAGAC 
ATACAGGTGC GCCGTGGGGA AGGTGTGACA TCCACTTCTC GCCGCATTGG ACCAGAACTA 
TCCAGGCGTA TCTACCTTCA GATGACCACT TTACAGCCAC ATCTCTCTTT AGATAGTGCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( XM_017024236.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879725.1 Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X1, mRNA.

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CloneID	OHu76307
Clone ID Related Accession (Same CDS sequence)	XM_017024236.1 , XM_017024236.2
Accession Version	XM_017024236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	crossover junction endonuclease EME1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGGAAGCC TGGACAGCAC TATGAAAGGG AAGGAAACGC TTCAGGGCTT TGTAACTGAC 
ATCACAGCAA AGACAGCAGG GAAAGCTCTG TCACTGGTGA TTGTGGATCA GGAGAAATGC 
TTCAGTGCTC AGAATCCTCC AAGAAGAGGG AAACAGGGAG CAAATAAACA GACCAAGAAG 
CAGCAGCAGA GACAACCAGA GGCCAGCATA GGGTCCATGG TATCCAGGGT AGACGCTGAA 
GAGGCATTGG TGGATCTGCA GCTACACACA GAAGCCCAGG CTCAAATTGT GCAGAGCTGG 
AAAGAGCTGG CCGACTTCAC ATGCGCATTC ACAAAGGCTG TGGCTGAGGC GCCCTTCAAG 
AAGCTCCGAG ATGAAACTAC CTTCTCCTTC TGTCTGGAGA GTGACTGGGC TGGAGGGGTG 
AAGGTGGACC TTGCTGGCAG GGGACTCGCA CTAGTCTGGA GGAGACAGAT TCAGCAGCTG 
AACCGAGTCA GCCTGGAAAT GGCCAGTGCA GTTGTGAATG CCTATCCCTC CCCACAGCTC 
CTGGTACAGG CTTATCAGCA GTGTTTTTCG GATAAAGAAC GCCAGAATTT GCTCGCAGAC 
ATACAGGTGC GCCGTGGGGA AGGTGTGACA TCCACTTCTC GCCGCATTGG ACCAGAACTA 
TCCAGGCGTA TCTACCTTCA GATGACCACT TTACAGCCAC ATCTCTCTTT AGATAGTGCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879725.1
CDS	74..1699
Translation	MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

Target ORF information:

RefSeq Version	XM_017024236.2
Organism	Homo sapiens(human)
Definition	Homo sapiens essential meiotic structure-specific endonuclease 1 (EME1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024236.2

ORF Insert Sequence:

ATGGCTCTAA AGAAGTCATC ACCCTCACTG GATTCTGGTG ATAGTGACTC TGAGGAGTTG 
CCAACATTTG CCTTTCTGAA GAAGGAACCA TCTTCAACAA AGAGGAGACA GCCTGAAAGG 
GAAGAGAAGA TTGTAGTGGT TGACATCTCA GATTGTGAAG CCTCCTGTCC TCCAGCACCA 
GAGTTATTTT CACCACCTGT CCCAGAAATA GCTGAAACTG TCACACAAAC ACAGCCAGTC 
AGGTTGCTAA GCAGTGAAAG TGAAGATGAA GAAGAATTTA TTCCTCTGGC TCAAAGGCTT 
ACATGTAAGT TTCTGACCCA CAAGCAACTG AGCCCTGAGG ACTCTAGCTC CCCAGTTAAA 
AGTGTTTTGG ATCATCAAAA TAATGAAGGT GCATCATGTG ACTGGAAAAA GCCCTTTCCA 
AAGATCCCTG AAGTTCCCCT CCATGATACC CCAGAGAGGA GTGCAGCAGA TAACAAGGAC 
CTGATCTTAG ATCCATGCTG TCAGCTTCCA GCCTACCTGT CTACCTGCCC TGGCCAGAGC 
AGCAGCTTGG CAGTAACCAA AACAAATTCT GACATCCTTC CACCCCAGAA GAAAACCAAG 
CCGAGTCAGA AGGTCCAGGG AAGAGGCTCA CACGGATGCC GGCAGCAGAG ACAAGCAAGG 
CAGAAGGAAA GCACCCTGAG AAGACAGGAA AGAAAGAATG CAGCACTGGT TACCAGGATG 
AAAGCCCAGA GGCCAGAGGA ATGCTTAAAA CACATCATTG TAGTGCTGGA TCCAGTGCTC 
TTACAGATGG AAGGTGGGGG CCAGCTCCTA GGAGCACTGC AGACCATGGA GTGCCGCTGT 
GTGATTGAGG CGCAGGCTGT GCCTTGCAGT GTCACTTGGA GGAGAAGGGC TGGGCCGTCT 
GAGGGAAGCC TGGACAGCAC TATGAAAGGG AAGGAAACGC TTCAGGGCTT TGTAACTGAC 
ATCACAGCAA AGACAGCAGG GAAAGCTCTG TCACTGGTGA TTGTGGATCA GGAGAAATGC 
TTCAGTGCTC AGAATCCTCC AAGAAGAGGG AAACAGGGAG CAAATAAACA GACCAAGAAG 
CAGCAGCAGA GACAACCAGA GGCCAGCATA GGGTCCATGG TATCCAGGGT AGACGCTGAA 
GAGGCATTGG TGGATCTGCA GCTACACACA GAAGCCCAGG CTCAAATTGT GCAGAGCTGG 
AAAGAGCTGG CCGACTTCAC ATGCGCATTC ACAAAGGCTG TGGCTGAGGC GCCCTTCAAG 
AAGCTCCGAG ATGAAACTAC CTTCTCCTTC TGTCTGGAGA GTGACTGGGC TGGAGGGGTG 
AAGGTGGACC TTGCTGGCAG GGGACTCGCA CTAGTCTGGA GGAGACAGAT TCAGCAGCTG 
AACCGAGTCA GCCTGGAAAT GGCCAGTGCA GTTGTGAATG CCTATCCCTC CCCACAGCTC 
CTGGTACAGG CTTATCAGCA GTGTTTTTCG GATAAAGAAC GCCAGAATTT GCTCGCAGAC 
ATACAGGTGC GCCGTGGGGA AGGTGTGACA TCCACTTCTC GCCGCATTGG ACCAGAACTA 
TCCAGGCGTA TCTACCTTCA GATGACCACT TTACAGCCAC ATCTCTCTTT AGATAGTGCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphorylation by CK2 regulates MUS81/EME1 in mitosis and after replication stress.
Nucleic acids research46(10)5109-5124(2018 06)
Palma A,Pugliese GM,Murfuni I,Marabitti V,Malacaria E,Rinalducci S,Minoprio A,Sanchez M,Mazzei F,Zolla L,Franchitto A,Pichierri P

Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage.
Cell reports20(7)1553-1562(2017 08)
Saugar I,Jiménez-Martín A,Tercero JA

SLX4-SLX1 Protein-independent Down-regulation of MUS81-EME1 Protein by HIV-1 Viral Protein R (Vpr).
The Journal of biological chemistry291(33)16936-16947(2016 08)
Zhou X,DeLucia M,Ahn J

Crystal structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates.
The EMBO journal33(9)1061-72(2014 May)
Gwon GH,Jo A,Baek K,Jin KS,Fu Y,Lee JB,Kim Y,Cho Y

Coordinated actions of SLX1-SLX4 and MUS81-EME1 for Holliday junction resolution in human cells.
Molecular cell52(2)234-47(2013 Oct)
Wyatt HD,Sarbajna S,Matos J,West SC

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EME1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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