EME1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	EME1
Entrez Gene ID	558350
Full Name	essential meiotic structure-specific endonuclease 1
Synonyms	fc30c07,wu:fc30c07
General protein information	Preferred Names essential meiotic structure-specific endonuclease 1 Names crossover junction endonuclease EME1 essential meiotic endonuclease 1 homolog 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_702004.5	XP_707096.1	crossover junction endonuclease EME1
XM_702004.6	XP_707096.1	crossover junction endonuclease EME1
XM_005156219.4	XP_005156276.1	crossover junction endonuclease EME1
XM_005156219.3	XP_005156276.1	crossover junction endonuclease EME1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002302	XP_312668.4
Mus musculus (house mouse)	Eme1	NP_808420.1
Xenopus tropicalis (tropical clawed frog)	eme1	NP_001072299.1
Rattus norvegicus (Norway rat)	Eme1	NP_001099300.2
Gallus gallus (chicken)	EME1	XP_003642406.1
Danio rerio (zebrafish)	eme1	XP_005156276.1
Drosophila melanogaster (fruit fly)	mms4	NP_610611.1
Homo sapiens (human)	EME1	NP_689676.2
Pan troglodytes (chimpanzee)	EME1	XP_001169575.1
Canis lupus familiaris (dog)	EME1	XP_005624696.1

Gene Ontology

The following EME1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EME1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17881	XM_702004.6 Latest version!	Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa17881	XM_005156219.4 Latest version!	Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa17881	XM_702004.5	Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa17881	XM_005156219.3	Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EME1 ( XM_702004.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_707096.1 Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17881
Clone ID Related Accession (Same CDS sequence)	XM_702004.5 , XM_702004.6 , XM_005156219.4 , XM_005156219.3
Accession Version	XM_702004.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	crossover junction endonuclease EME1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_702004.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGCG TCAGCAGACC TGGATTCGTC TTAAGTGACA GTGAGTCGAG TGATTCAGAA 
GAGCTGCCTG TGTTCGATTT CTCTCAGTCA CAAACCAGAC AGACCAACTT GGTGGTTTTA 
GACAGTTCAG ACTCAGAAAT GGCTCCTGTT GCAAATCCAG TTTCATCTCT GGAGGTCCAA 
ACTTCTGCTG GAGCCACTAA AAGTGATGTG CTGATGGTCA GCAGTGCTAG CGAGGATGAG 
GAGGAGCCCA TGATTCCCTT AGCTGTGAGA CTCAAACAGA AACAGTGTGG TCTGTCCATA 
TCCGCCACCA ATGCAGAACA GAGCCGGGCT TCCAATGCAG TTTCAAATGG ATGTTCACGC 
CTCTTAGATG TTCCAGCATA TCATATCAAC CCTGAGGCCC AGCCAGTTCA ACGCAACAAG 
ATCAGTAGCA ATATAACAAG GCATTACAGC TCAAACCATG ATGCACCATA CCTCACAAAA 
GAACCTGAAG CAGAGAATGG CACTTACCTG GCCAAACGCA AGAAAACCCC AGCAGAAGTG 
GAGGCTGCCA GACAAGAAGC CCTGAGGAAA AGAGCAATTC GAGAGCACCA ACAGGAAGAG 
AGGGGGAGAT TGAGGATGGA GAAGAAAGCT CTGGCTGATG CTGTGAAAGC CCTAAGGCCA 
GAGGAGTGCA TCAAACACAC GGTGGTGACG GTCGACCCTG GTCTGTTGCA GCTGGAAGGT 
GGCGGCGCTC TCCTGACTTC CCTGCATGCC ATGGGCTGCA ACTGTGCCAT AGAGAAGCAG 
TCTCTTCCTC GCAGTGTCTG CTGGGCGAGA CGCTCGCCCT GCCCTCAGAG TGGTGAGATG 
AAGTCTTTTC CAGAGTCATA TACTATCATC CAAGTTCCAG TGGATGACTT TGTGACCATG 
ATTAACAGCT ATTGCAAGAG GCAAAGCAGC AGTGTTTTGA CTGATACTGG GATATCACTG 
ACTGCCTGGA TACAAGGACT TATGAGCAGG AATCCAGGCA GATCCCTCAG TCTAGTTGTG 
ATTGACATAG AGAAGTACTT CAGATCCCAG AATTCAAAAT GTCAGAAGAA ATACCGTGAG 
GCAGTGCTGG GCGAGGAGAA GAATGTGGGA CTTCAGGGAG GGCAGAAAAA GAGAAGAAAG 
AAAGACGATA TCAATCAGCT TCCTGAGGTC TCGCGAGTGC AGGTGGAGGA GGCTTTGGTA 
GACCTGCAGT TGCAAACCGG GGTGCAAGTT CGCTTCCTCT CTACCTGGAA AGACTTCACT 
GATTATATTA CCATGTCAAC CAAAGCAGTA GCTGAGGCGC CGTTCAAGCG GGAGCGAGAG 
AAGACGGGAT TTACGTTTTG TTTGGAGAGT GAGTGGGCTG GAGGTCAGAA GGTAGACCGC 
ACAGGGAATG GCCTTCTGCA AGTTTGGAAG AGACAAATAC AGCAGTTGAA CCGCGTCAGC 
CTTGATATGG CCAGTGCTAT ACTCAGTGCC TACCCCTCTC CTCGGCTTCT TGCTCAGGCA 
TACACACGGT GCAGATCTGA ACAGGAAAAA GTTGGCTTGT TGTCAGACTT ACTCATTCGT 
CGTGGAGAGG GTGTGACATC TACAACCCGT AGAGTCGGTC CAGAACTTTC CAAACGAGTC 
TATCTTATGA TGACCTCAAG TGATGACCAA CAACCCCTTG ATTCTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_707096.1
CDS	153..1823
Translation	MTCVSRPGFVLSDSESSDSEELPVFDFSQSQTRQTNLVVLDSSDSEMAPVANPVSSLEVQTSAGATKSDVLMVSSASEDEEEPMIPLAVRLKQKQCGLSISATNAEQSRASNAVSNGCSRLLDVPAYHINPEAQPVQRNKISSNITRHYSSNHDAPYLTKEPEAENGTYLAKRKKTPAEVEAARQEALRKRAIREHQQEERGRLRMEKKALADAVKALRPEECIKHTVVTVDPGLLQLEGGGALLTSLHAMGCNCAIEKQSLPRSVCWARRSPCPQSGEMKSFPESYTIIQVPVDDFVTMINSYCKRQSSSVLTDTGISLTAWIQGLMSRNPGRSLSLVVIDIEKYFRSQNSKCQKKYREAVLGEEKNVGLQGGQKKRRKKDDINQLPEVSRVQVEEALVDLQLQTGVQVRFLSTWKDFTDYITMSTKAVAEAPFKREREKTGFTFCLESEWAGGQKVDRTGNGLLQVWKRQIQQLNRVSLDMASAILSAYPSPRLLAQAYTRCRSEQEKVGLLSDLLIRRGEGVTSTTRRVGPELSKRVYLMMTSSDDQQPLDSA

Target ORF information:

RefSeq Version	XM_702004.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_702004.5

ORF Insert Sequence:

ATGACTTGCG TCAGCAGACC TGGATTCGTC TTAAGTGACA GTGAGTCGAG TGATTCAGAA 
GAGCTGCCTG TGTTCGATTT CTCTCAGTCA CAAACCAGAC AGACCAACTT GGTGGTTTTA 
GACAGTTCAG ACTCAGAAAT GGCTCCTGTT GCAAATCCAG TTTCATCTCT GGAGGTCCAA 
ACTTCTGCTG GAGCCACTAA AAGTGATGTG CTGATGGTCA GCAGTGCTAG CGAGGATGAG 
GAGGAGCCCA TGATTCCCTT AGCTGTGAGA CTCAAACAGA AACAGTGTGG TCTGTCCATA 
TCCGCCACCA ATGCAGAACA GAGCCGGGCT TCCAATGCAG TTTCAAATGG ATGTTCACGC 
CTCTTAGATG TTCCAGCATA TCATATCAAC CCTGAGGCCC AGCCAGTTCA ACGCAACAAG 
ATCAGTAGCA ATATAACAAG GCATTACAGC TCAAACCATG ATGCACCATA CCTCACAAAA 
GAACCTGAAG CAGAGAATGG CACTTACCTG GCCAAACGCA AGAAAACCCC AGCAGAAGTG 
GAGGCTGCCA GACAAGAAGC CCTGAGGAAA AGAGCAATTC GAGAGCACCA ACAGGAAGAG 
AGGGGGAGAT TGAGGATGGA GAAGAAAGCT CTGGCTGATG CTGTGAAAGC CCTAAGGCCA 
GAGGAGTGCA TCAAACACAC GGTGGTGACG GTCGACCCTG GTCTGTTGCA GCTGGAAGGT 
GGCGGCGCTC TCCTGACTTC CCTGCATGCC ATGGGCTGCA ACTGTGCCAT AGAGAAGCAG 
TCTCTTCCTC GCAGTGTCTG CTGGGCGAGA CGCTCGCCCT GCCCTCAGAG TGGTGAGATG 
AAGTCTTTTC CAGAGTCATA TACTATCATC CAAGTTCCAG TGGATGACTT TGTGACCATG 
ATTAACAGCT ATTGCAAGAG GCAAAGCAGC AGTGTTTTGA CTGATACTGG GATATCACTG 
ACTGCCTGGA TACAAGGACT TATGAGCAGG AATCCAGGCA GATCCCTCAG TCTAGTTGTG 
ATTGACATAG AGAAGTACTT CAGATCCCAG AATTCAAAAT GTCAGAAGAA ATACCGTGAG 
GCAGTGCTGG GCGAGGAGAA GAATGTGGGA CTTCAGGGAG GGCAGAAAAA GAGAAGAAAG 
AAAGACGATA TCAATCAGCT TCCTGAGGTC TCGCGAGTGC AGGTGGAGGA GGCTTTGGTA 
GACCTGCAGT TGCAAACCGG GGTGCAAGTT CGCTTCCTCT CTACCTGGAA AGACTTCACT 
GATTATATTA CCATGTCAAC CAAAGCAGTA GCTGAGGCGC CGTTCAAGCG GGAGCGAGAG 
AAGACGGGAT TTACGTTTTG TTTGGAGAGT GAGTGGGCTG GAGGTCAGAA GGTAGACCGC 
ACAGGGAATG GCCTTCTGCA AGTTTGGAAG AGACAAATAC AGCAGTTGAA CCGCGTCAGC 
CTTGATATGG CCAGTGCTAT ACTCAGTGCC TACCCCTCTC CTCGGCTTCT TGCTCAGGCA 
TACACACGGT GCAGATCTGA ACAGGAAAAA GTTGGCTTGT TGTCAGACTT ACTCATTCGT 
CGTGGAGAGG GTGTGACATC TACAACCCGT AGAGTCGGTC CAGAACTTTC CAAACGAGTC 
TATCTTATGA TGACCTCAAG TGATGACCAA CAACCCCTTG ATTCTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( XM_702004.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_707096.1 Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17881
Clone ID Related Accession (Same CDS sequence)	XM_702004.5 , XM_702004.6 , XM_005156219.4 , XM_005156219.3
Accession Version	XM_702004.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	crossover junction endonuclease EME1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_702004.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGCG TCAGCAGACC TGGATTCGTC TTAAGTGACA GTGAGTCGAG TGATTCAGAA 
GAGCTGCCTG TGTTCGATTT CTCTCAGTCA CAAACCAGAC AGACCAACTT GGTGGTTTTA 
GACAGTTCAG ACTCAGAAAT GGCTCCTGTT GCAAATCCAG TTTCATCTCT GGAGGTCCAA 
ACTTCTGCTG GAGCCACTAA AAGTGATGTG CTGATGGTCA GCAGTGCTAG CGAGGATGAG 
GAGGAGCCCA TGATTCCCTT AGCTGTGAGA CTCAAACAGA AACAGTGTGG TCTGTCCATA 
TCCGCCACCA ATGCAGAACA GAGCCGGGCT TCCAATGCAG TTTCAAATGG ATGTTCACGC 
CTCTTAGATG TTCCAGCATA TCATATCAAC CCTGAGGCCC AGCCAGTTCA ACGCAACAAG 
ATCAGTAGCA ATATAACAAG GCATTACAGC TCAAACCATG ATGCACCATA CCTCACAAAA 
GAACCTGAAG CAGAGAATGG CACTTACCTG GCCAAACGCA AGAAAACCCC AGCAGAAGTG 
GAGGCTGCCA GACAAGAAGC CCTGAGGAAA AGAGCAATTC GAGAGCACCA ACAGGAAGAG 
AGGGGGAGAT TGAGGATGGA GAAGAAAGCT CTGGCTGATG CTGTGAAAGC CCTAAGGCCA 
GAGGAGTGCA TCAAACACAC GGTGGTGACG GTCGACCCTG GTCTGTTGCA GCTGGAAGGT 
GGCGGCGCTC TCCTGACTTC CCTGCATGCC ATGGGCTGCA ACTGTGCCAT AGAGAAGCAG 
TCTCTTCCTC GCAGTGTCTG CTGGGCGAGA CGCTCGCCCT GCCCTCAGAG TGGTGAGATG 
AAGTCTTTTC CAGAGTCATA TACTATCATC CAAGTTCCAG TGGATGACTT TGTGACCATG 
ATTAACAGCT ATTGCAAGAG GCAAAGCAGC AGTGTTTTGA CTGATACTGG GATATCACTG 
ACTGCCTGGA TACAAGGACT TATGAGCAGG AATCCAGGCA GATCCCTCAG TCTAGTTGTG 
ATTGACATAG AGAAGTACTT CAGATCCCAG AATTCAAAAT GTCAGAAGAA ATACCGTGAG 
GCAGTGCTGG GCGAGGAGAA GAATGTGGGA CTTCAGGGAG GGCAGAAAAA GAGAAGAAAG 
AAAGACGATA TCAATCAGCT TCCTGAGGTC TCGCGAGTGC AGGTGGAGGA GGCTTTGGTA 
GACCTGCAGT TGCAAACCGG GGTGCAAGTT CGCTTCCTCT CTACCTGGAA AGACTTCACT 
GATTATATTA CCATGTCAAC CAAAGCAGTA GCTGAGGCGC CGTTCAAGCG GGAGCGAGAG 
AAGACGGGAT TTACGTTTTG TTTGGAGAGT GAGTGGGCTG GAGGTCAGAA GGTAGACCGC 
ACAGGGAATG GCCTTCTGCA AGTTTGGAAG AGACAAATAC AGCAGTTGAA CCGCGTCAGC 
CTTGATATGG CCAGTGCTAT ACTCAGTGCC TACCCCTCTC CTCGGCTTCT TGCTCAGGCA 
TACACACGGT GCAGATCTGA ACAGGAAAAA GTTGGCTTGT TGTCAGACTT ACTCATTCGT 
CGTGGAGAGG GTGTGACATC TACAACCCGT AGAGTCGGTC CAGAACTTTC CAAACGAGTC 
TATCTTATGA TGACCTCAAG TGATGACCAA CAACCCCTTG ATTCTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_707096.1
CDS	158..1828
Translation	MTCVSRPGFVLSDSESSDSEELPVFDFSQSQTRQTNLVVLDSSDSEMAPVANPVSSLEVQTSAGATKSDVLMVSSASEDEEEPMIPLAVRLKQKQCGLSISATNAEQSRASNAVSNGCSRLLDVPAYHINPEAQPVQRNKISSNITRHYSSNHDAPYLTKEPEAENGTYLAKRKKTPAEVEAARQEALRKRAIREHQQEERGRLRMEKKALADAVKALRPEECIKHTVVTVDPGLLQLEGGGALLTSLHAMGCNCAIEKQSLPRSVCWARRSPCPQSGEMKSFPESYTIIQVPVDDFVTMINSYCKRQSSSVLTDTGISLTAWIQGLMSRNPGRSLSLVVIDIEKYFRSQNSKCQKKYREAVLGEEKNVGLQGGQKKRRKKDDINQLPEVSRVQVEEALVDLQLQTGVQVRFLSTWKDFTDYITMSTKAVAEAPFKREREKTGFTFCLESEWAGGQKVDRTGNGLLQVWKRQIQQLNRVSLDMASAILSAYPSPRLLAQAYTRCRSEQEKVGLLSDLLIRRGEGVTSTTRRVGPELSKRVYLMMTSSDDQQPLDSA

Target ORF information:

RefSeq Version	XM_702004.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_702004.6

ORF Insert Sequence:

ATGACTTGCG TCAGCAGACC TGGATTCGTC TTAAGTGACA GTGAGTCGAG TGATTCAGAA 
GAGCTGCCTG TGTTCGATTT CTCTCAGTCA CAAACCAGAC AGACCAACTT GGTGGTTTTA 
GACAGTTCAG ACTCAGAAAT GGCTCCTGTT GCAAATCCAG TTTCATCTCT GGAGGTCCAA 
ACTTCTGCTG GAGCCACTAA AAGTGATGTG CTGATGGTCA GCAGTGCTAG CGAGGATGAG 
GAGGAGCCCA TGATTCCCTT AGCTGTGAGA CTCAAACAGA AACAGTGTGG TCTGTCCATA 
TCCGCCACCA ATGCAGAACA GAGCCGGGCT TCCAATGCAG TTTCAAATGG ATGTTCACGC 
CTCTTAGATG TTCCAGCATA TCATATCAAC CCTGAGGCCC AGCCAGTTCA ACGCAACAAG 
ATCAGTAGCA ATATAACAAG GCATTACAGC TCAAACCATG ATGCACCATA CCTCACAAAA 
GAACCTGAAG CAGAGAATGG CACTTACCTG GCCAAACGCA AGAAAACCCC AGCAGAAGTG 
GAGGCTGCCA GACAAGAAGC CCTGAGGAAA AGAGCAATTC GAGAGCACCA ACAGGAAGAG 
AGGGGGAGAT TGAGGATGGA GAAGAAAGCT CTGGCTGATG CTGTGAAAGC CCTAAGGCCA 
GAGGAGTGCA TCAAACACAC GGTGGTGACG GTCGACCCTG GTCTGTTGCA GCTGGAAGGT 
GGCGGCGCTC TCCTGACTTC CCTGCATGCC ATGGGCTGCA ACTGTGCCAT AGAGAAGCAG 
TCTCTTCCTC GCAGTGTCTG CTGGGCGAGA CGCTCGCCCT GCCCTCAGAG TGGTGAGATG 
AAGTCTTTTC CAGAGTCATA TACTATCATC CAAGTTCCAG TGGATGACTT TGTGACCATG 
ATTAACAGCT ATTGCAAGAG GCAAAGCAGC AGTGTTTTGA CTGATACTGG GATATCACTG 
ACTGCCTGGA TACAAGGACT TATGAGCAGG AATCCAGGCA GATCCCTCAG TCTAGTTGTG 
ATTGACATAG AGAAGTACTT CAGATCCCAG AATTCAAAAT GTCAGAAGAA ATACCGTGAG 
GCAGTGCTGG GCGAGGAGAA GAATGTGGGA CTTCAGGGAG GGCAGAAAAA GAGAAGAAAG 
AAAGACGATA TCAATCAGCT TCCTGAGGTC TCGCGAGTGC AGGTGGAGGA GGCTTTGGTA 
GACCTGCAGT TGCAAACCGG GGTGCAAGTT CGCTTCCTCT CTACCTGGAA AGACTTCACT 
GATTATATTA CCATGTCAAC CAAAGCAGTA GCTGAGGCGC CGTTCAAGCG GGAGCGAGAG 
AAGACGGGAT TTACGTTTTG TTTGGAGAGT GAGTGGGCTG GAGGTCAGAA GGTAGACCGC 
ACAGGGAATG GCCTTCTGCA AGTTTGGAAG AGACAAATAC AGCAGTTGAA CCGCGTCAGC 
CTTGATATGG CCAGTGCTAT ACTCAGTGCC TACCCCTCTC CTCGGCTTCT TGCTCAGGCA 
TACACACGGT GCAGATCTGA ACAGGAAAAA GTTGGCTTGT TGTCAGACTT ACTCATTCGT 
CGTGGAGAGG GTGTGACATC TACAACCCGT AGAGTCGGTC CAGAACTTTC CAAACGAGTC 
TATCTTATGA TGACCTCAAG TGATGACCAA CAACCCCTTG ATTCTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( XM_005156219.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156276.1 Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17881
Clone ID Related Accession (Same CDS sequence)	XM_702004.5 , XM_702004.6 , XM_005156219.4 , XM_005156219.3
Accession Version	XM_005156219.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	crossover junction endonuclease EME1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156219.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGCG TCAGCAGACC TGGATTCGTC TTAAGTGACA GTGAGTCGAG TGATTCAGAA 
GAGCTGCCTG TGTTCGATTT CTCTCAGTCA CAAACCAGAC AGACCAACTT GGTGGTTTTA 
GACAGTTCAG ACTCAGAAAT GGCTCCTGTT GCAAATCCAG TTTCATCTCT GGAGGTCCAA 
ACTTCTGCTG GAGCCACTAA AAGTGATGTG CTGATGGTCA GCAGTGCTAG CGAGGATGAG 
GAGGAGCCCA TGATTCCCTT AGCTGTGAGA CTCAAACAGA AACAGTGTGG TCTGTCCATA 
TCCGCCACCA ATGCAGAACA GAGCCGGGCT TCCAATGCAG TTTCAAATGG ATGTTCACGC 
CTCTTAGATG TTCCAGCATA TCATATCAAC CCTGAGGCCC AGCCAGTTCA ACGCAACAAG 
ATCAGTAGCA ATATAACAAG GCATTACAGC TCAAACCATG ATGCACCATA CCTCACAAAA 
GAACCTGAAG CAGAGAATGG CACTTACCTG GCCAAACGCA AGAAAACCCC AGCAGAAGTG 
GAGGCTGCCA GACAAGAAGC CCTGAGGAAA AGAGCAATTC GAGAGCACCA ACAGGAAGAG 
AGGGGGAGAT TGAGGATGGA GAAGAAAGCT CTGGCTGATG CTGTGAAAGC CCTAAGGCCA 
GAGGAGTGCA TCAAACACAC GGTGGTGACG GTCGACCCTG GTCTGTTGCA GCTGGAAGGT 
GGCGGCGCTC TCCTGACTTC CCTGCATGCC ATGGGCTGCA ACTGTGCCAT AGAGAAGCAG 
TCTCTTCCTC GCAGTGTCTG CTGGGCGAGA CGCTCGCCCT GCCCTCAGAG TGGTGAGATG 
AAGTCTTTTC CAGAGTCATA TACTATCATC CAAGTTCCAG TGGATGACTT TGTGACCATG 
ATTAACAGCT ATTGCAAGAG GCAAAGCAGC AGTGTTTTGA CTGATACTGG GATATCACTG 
ACTGCCTGGA TACAAGGACT TATGAGCAGG AATCCAGGCA GATCCCTCAG TCTAGTTGTG 
ATTGACATAG AGAAGTACTT CAGATCCCAG AATTCAAAAT GTCAGAAGAA ATACCGTGAG 
GCAGTGCTGG GCGAGGAGAA GAATGTGGGA CTTCAGGGAG GGCAGAAAAA GAGAAGAAAG 
AAAGACGATA TCAATCAGCT TCCTGAGGTC TCGCGAGTGC AGGTGGAGGA GGCTTTGGTA 
GACCTGCAGT TGCAAACCGG GGTGCAAGTT CGCTTCCTCT CTACCTGGAA AGACTTCACT 
GATTATATTA CCATGTCAAC CAAAGCAGTA GCTGAGGCGC CGTTCAAGCG GGAGCGAGAG 
AAGACGGGAT TTACGTTTTG TTTGGAGAGT GAGTGGGCTG GAGGTCAGAA GGTAGACCGC 
ACAGGGAATG GCCTTCTGCA AGTTTGGAAG AGACAAATAC AGCAGTTGAA CCGCGTCAGC 
CTTGATATGG CCAGTGCTAT ACTCAGTGCC TACCCCTCTC CTCGGCTTCT TGCTCAGGCA 
TACACACGGT GCAGATCTGA ACAGGAAAAA GTTGGCTTGT TGTCAGACTT ACTCATTCGT 
CGTGGAGAGG GTGTGACATC TACAACCCGT AGAGTCGGTC CAGAACTTTC CAAACGAGTC 
TATCTTATGA TGACCTCAAG TGATGACCAA CAACCCCTTG ATTCTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156276.1
CDS	88..1758
Translation	MTCVSRPGFVLSDSESSDSEELPVFDFSQSQTRQTNLVVLDSSDSEMAPVANPVSSLEVQTSAGATKSDVLMVSSASEDEEEPMIPLAVRLKQKQCGLSISATNAEQSRASNAVSNGCSRLLDVPAYHINPEAQPVQRNKISSNITRHYSSNHDAPYLTKEPEAENGTYLAKRKKTPAEVEAARQEALRKRAIREHQQEERGRLRMEKKALADAVKALRPEECIKHTVVTVDPGLLQLEGGGALLTSLHAMGCNCAIEKQSLPRSVCWARRSPCPQSGEMKSFPESYTIIQVPVDDFVTMINSYCKRQSSSVLTDTGISLTAWIQGLMSRNPGRSLSLVVIDIEKYFRSQNSKCQKKYREAVLGEEKNVGLQGGQKKRRKKDDINQLPEVSRVQVEEALVDLQLQTGVQVRFLSTWKDFTDYITMSTKAVAEAPFKREREKTGFTFCLESEWAGGQKVDRTGNGLLQVWKRQIQQLNRVSLDMASAILSAYPSPRLLAQAYTRCRSEQEKVGLLSDLLIRRGEGVTSTTRRVGPELSKRVYLMMTSSDDQQPLDSA

Target ORF information:

RefSeq Version	XM_005156219.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156219.4

ORF Insert Sequence:

ATGACTTGCG TCAGCAGACC TGGATTCGTC TTAAGTGACA GTGAGTCGAG TGATTCAGAA 
GAGCTGCCTG TGTTCGATTT CTCTCAGTCA CAAACCAGAC AGACCAACTT GGTGGTTTTA 
GACAGTTCAG ACTCAGAAAT GGCTCCTGTT GCAAATCCAG TTTCATCTCT GGAGGTCCAA 
ACTTCTGCTG GAGCCACTAA AAGTGATGTG CTGATGGTCA GCAGTGCTAG CGAGGATGAG 
GAGGAGCCCA TGATTCCCTT AGCTGTGAGA CTCAAACAGA AACAGTGTGG TCTGTCCATA 
TCCGCCACCA ATGCAGAACA GAGCCGGGCT TCCAATGCAG TTTCAAATGG ATGTTCACGC 
CTCTTAGATG TTCCAGCATA TCATATCAAC CCTGAGGCCC AGCCAGTTCA ACGCAACAAG 
ATCAGTAGCA ATATAACAAG GCATTACAGC TCAAACCATG ATGCACCATA CCTCACAAAA 
GAACCTGAAG CAGAGAATGG CACTTACCTG GCCAAACGCA AGAAAACCCC AGCAGAAGTG 
GAGGCTGCCA GACAAGAAGC CCTGAGGAAA AGAGCAATTC GAGAGCACCA ACAGGAAGAG 
AGGGGGAGAT TGAGGATGGA GAAGAAAGCT CTGGCTGATG CTGTGAAAGC CCTAAGGCCA 
GAGGAGTGCA TCAAACACAC GGTGGTGACG GTCGACCCTG GTCTGTTGCA GCTGGAAGGT 
GGCGGCGCTC TCCTGACTTC CCTGCATGCC ATGGGCTGCA ACTGTGCCAT AGAGAAGCAG 
TCTCTTCCTC GCAGTGTCTG CTGGGCGAGA CGCTCGCCCT GCCCTCAGAG TGGTGAGATG 
AAGTCTTTTC CAGAGTCATA TACTATCATC CAAGTTCCAG TGGATGACTT TGTGACCATG 
ATTAACAGCT ATTGCAAGAG GCAAAGCAGC AGTGTTTTGA CTGATACTGG GATATCACTG 
ACTGCCTGGA TACAAGGACT TATGAGCAGG AATCCAGGCA GATCCCTCAG TCTAGTTGTG 
ATTGACATAG AGAAGTACTT CAGATCCCAG AATTCAAAAT GTCAGAAGAA ATACCGTGAG 
GCAGTGCTGG GCGAGGAGAA GAATGTGGGA CTTCAGGGAG GGCAGAAAAA GAGAAGAAAG 
AAAGACGATA TCAATCAGCT TCCTGAGGTC TCGCGAGTGC AGGTGGAGGA GGCTTTGGTA 
GACCTGCAGT TGCAAACCGG GGTGCAAGTT CGCTTCCTCT CTACCTGGAA AGACTTCACT 
GATTATATTA CCATGTCAAC CAAAGCAGTA GCTGAGGCGC CGTTCAAGCG GGAGCGAGAG 
AAGACGGGAT TTACGTTTTG TTTGGAGAGT GAGTGGGCTG GAGGTCAGAA GGTAGACCGC 
ACAGGGAATG GCCTTCTGCA AGTTTGGAAG AGACAAATAC AGCAGTTGAA CCGCGTCAGC 
CTTGATATGG CCAGTGCTAT ACTCAGTGCC TACCCCTCTC CTCGGCTTCT TGCTCAGGCA 
TACACACGGT GCAGATCTGA ACAGGAAAAA GTTGGCTTGT TGTCAGACTT ACTCATTCGT 
CGTGGAGAGG GTGTGACATC TACAACCCGT AGAGTCGGTC CAGAACTTTC CAAACGAGTC 
TATCTTATGA TGACCTCAAG TGATGACCAA CAACCCCTTG ATTCTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EME1 ( XM_005156219.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156276.1 Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X2, mRNA.

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CloneID	ODa17881
Clone ID Related Accession (Same CDS sequence)	XM_702004.5 , XM_702004.6 , XM_005156219.4 , XM_005156219.3
Accession Version	XM_005156219.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	crossover junction endonuclease EME1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGCG TCAGCAGACC TGGATTCGTC TTAAGTGACA GTGAGTCGAG TGATTCAGAA 
GAGCTGCCTG TGTTCGATTT CTCTCAGTCA CAAACCAGAC AGACCAACTT GGTGGTTTTA 
GACAGTTCAG ACTCAGAAAT GGCTCCTGTT GCAAATCCAG TTTCATCTCT GGAGGTCCAA 
ACTTCTGCTG GAGCCACTAA AAGTGATGTG CTGATGGTCA GCAGTGCTAG CGAGGATGAG 
GAGGAGCCCA TGATTCCCTT AGCTGTGAGA CTCAAACAGA AACAGTGTGG TCTGTCCATA 
TCCGCCACCA ATGCAGAACA GAGCCGGGCT TCCAATGCAG TTTCAAATGG ATGTTCACGC 
CTCTTAGATG TTCCAGCATA TCATATCAAC CCTGAGGCCC AGCCAGTTCA ACGCAACAAG 
ATCAGTAGCA ATATAACAAG GCATTACAGC TCAAACCATG ATGCACCATA CCTCACAAAA 
GAACCTGAAG CAGAGAATGG CACTTACCTG GCCAAACGCA AGAAAACCCC AGCAGAAGTG 
GAGGCTGCCA GACAAGAAGC CCTGAGGAAA AGAGCAATTC GAGAGCACCA ACAGGAAGAG 
AGGGGGAGAT TGAGGATGGA GAAGAAAGCT CTGGCTGATG CTGTGAAAGC CCTAAGGCCA 
GAGGAGTGCA TCAAACACAC GGTGGTGACG GTCGACCCTG GTCTGTTGCA GCTGGAAGGT 
GGCGGCGCTC TCCTGACTTC CCTGCATGCC ATGGGCTGCA ACTGTGCCAT AGAGAAGCAG 
TCTCTTCCTC GCAGTGTCTG CTGGGCGAGA CGCTCGCCCT GCCCTCAGAG TGGTGAGATG 
AAGTCTTTTC CAGAGTCATA TACTATCATC CAAGTTCCAG TGGATGACTT TGTGACCATG 
ATTAACAGCT ATTGCAAGAG GCAAAGCAGC AGTGTTTTGA CTGATACTGG GATATCACTG 
ACTGCCTGGA TACAAGGACT TATGAGCAGG AATCCAGGCA GATCCCTCAG TCTAGTTGTG 
ATTGACATAG AGAAGTACTT CAGATCCCAG AATTCAAAAT GTCAGAAGAA ATACCGTGAG 
GCAGTGCTGG GCGAGGAGAA GAATGTGGGA CTTCAGGGAG GGCAGAAAAA GAGAAGAAAG 
AAAGACGATA TCAATCAGCT TCCTGAGGTC TCGCGAGTGC AGGTGGAGGA GGCTTTGGTA 
GACCTGCAGT TGCAAACCGG GGTGCAAGTT CGCTTCCTCT CTACCTGGAA AGACTTCACT 
GATTATATTA CCATGTCAAC CAAAGCAGTA GCTGAGGCGC CGTTCAAGCG GGAGCGAGAG 
AAGACGGGAT TTACGTTTTG TTTGGAGAGT GAGTGGGCTG GAGGTCAGAA GGTAGACCGC 
ACAGGGAATG GCCTTCTGCA AGTTTGGAAG AGACAAATAC AGCAGTTGAA CCGCGTCAGC 
CTTGATATGG CCAGTGCTAT ACTCAGTGCC TACCCCTCTC CTCGGCTTCT TGCTCAGGCA 
TACACACGGT GCAGATCTGA ACAGGAAAAA GTTGGCTTGT TGTCAGACTT ACTCATTCGT 
CGTGGAGAGG GTGTGACATC TACAACCCGT AGAGTCGGTC CAGAACTTTC CAAACGAGTC 
TATCTTATGA TGACCTCAAG TGATGACCAA CAACCCCTTG ATTCTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156276.1
CDS	92..1762
Translation	MTCVSRPGFVLSDSESSDSEELPVFDFSQSQTRQTNLVVLDSSDSEMAPVANPVSSLEVQTSAGATKSDVLMVSSASEDEEEPMIPLAVRLKQKQCGLSISATNAEQSRASNAVSNGCSRLLDVPAYHINPEAQPVQRNKISSNITRHYSSNHDAPYLTKEPEAENGTYLAKRKKTPAEVEAARQEALRKRAIREHQQEERGRLRMEKKALADAVKALRPEECIKHTVVTVDPGLLQLEGGGALLTSLHAMGCNCAIEKQSLPRSVCWARRSPCPQSGEMKSFPESYTIIQVPVDDFVTMINSYCKRQSSSVLTDTGISLTAWIQGLMSRNPGRSLSLVVIDIEKYFRSQNSKCQKKYREAVLGEEKNVGLQGGQKKRRKKDDINQLPEVSRVQVEEALVDLQLQTGVQVRFLSTWKDFTDYITMSTKAVAEAPFKREREKTGFTFCLESEWAGGQKVDRTGNGLLQVWKRQIQQLNRVSLDMASAILSAYPSPRLLAQAYTRCRSEQEKVGLLSDLLIRRGEGVTSTTRRVGPELSKRVYLMMTSSDDQQPLDSA

Target ORF information:

RefSeq Version	XM_005156219.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio essential meiotic structure-specific endonuclease 1 (eme1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156219.3

ORF Insert Sequence:

ATGACTTGCG TCAGCAGACC TGGATTCGTC TTAAGTGACA GTGAGTCGAG TGATTCAGAA 
GAGCTGCCTG TGTTCGATTT CTCTCAGTCA CAAACCAGAC AGACCAACTT GGTGGTTTTA 
GACAGTTCAG ACTCAGAAAT GGCTCCTGTT GCAAATCCAG TTTCATCTCT GGAGGTCCAA 
ACTTCTGCTG GAGCCACTAA AAGTGATGTG CTGATGGTCA GCAGTGCTAG CGAGGATGAG 
GAGGAGCCCA TGATTCCCTT AGCTGTGAGA CTCAAACAGA AACAGTGTGG TCTGTCCATA 
TCCGCCACCA ATGCAGAACA GAGCCGGGCT TCCAATGCAG TTTCAAATGG ATGTTCACGC 
CTCTTAGATG TTCCAGCATA TCATATCAAC CCTGAGGCCC AGCCAGTTCA ACGCAACAAG 
ATCAGTAGCA ATATAACAAG GCATTACAGC TCAAACCATG ATGCACCATA CCTCACAAAA 
GAACCTGAAG CAGAGAATGG CACTTACCTG GCCAAACGCA AGAAAACCCC AGCAGAAGTG 
GAGGCTGCCA GACAAGAAGC CCTGAGGAAA AGAGCAATTC GAGAGCACCA ACAGGAAGAG 
AGGGGGAGAT TGAGGATGGA GAAGAAAGCT CTGGCTGATG CTGTGAAAGC CCTAAGGCCA 
GAGGAGTGCA TCAAACACAC GGTGGTGACG GTCGACCCTG GTCTGTTGCA GCTGGAAGGT 
GGCGGCGCTC TCCTGACTTC CCTGCATGCC ATGGGCTGCA ACTGTGCCAT AGAGAAGCAG 
TCTCTTCCTC GCAGTGTCTG CTGGGCGAGA CGCTCGCCCT GCCCTCAGAG TGGTGAGATG 
AAGTCTTTTC CAGAGTCATA TACTATCATC CAAGTTCCAG TGGATGACTT TGTGACCATG 
ATTAACAGCT ATTGCAAGAG GCAAAGCAGC AGTGTTTTGA CTGATACTGG GATATCACTG 
ACTGCCTGGA TACAAGGACT TATGAGCAGG AATCCAGGCA GATCCCTCAG TCTAGTTGTG 
ATTGACATAG AGAAGTACTT CAGATCCCAG AATTCAAAAT GTCAGAAGAA ATACCGTGAG 
GCAGTGCTGG GCGAGGAGAA GAATGTGGGA CTTCAGGGAG GGCAGAAAAA GAGAAGAAAG 
AAAGACGATA TCAATCAGCT TCCTGAGGTC TCGCGAGTGC AGGTGGAGGA GGCTTTGGTA 
GACCTGCAGT TGCAAACCGG GGTGCAAGTT CGCTTCCTCT CTACCTGGAA AGACTTCACT 
GATTATATTA CCATGTCAAC CAAAGCAGTA GCTGAGGCGC CGTTCAAGCG GGAGCGAGAG 
AAGACGGGAT TTACGTTTTG TTTGGAGAGT GAGTGGGCTG GAGGTCAGAA GGTAGACCGC 
ACAGGGAATG GCCTTCTGCA AGTTTGGAAG AGACAAATAC AGCAGTTGAA CCGCGTCAGC 
CTTGATATGG CCAGTGCTAT ACTCAGTGCC TACCCCTCTC CTCGGCTTCT TGCTCAGGCA 
TACACACGGT GCAGATCTGA ACAGGAAAAA GTTGGCTTGT TGTCAGACTT ACTCATTCGT 
CGTGGAGAGG GTGTGACATC TACAACCCGT AGAGTCGGTC CAGAACTTTC CAAACGAGTC 
TATCTTATGA TGACCTCAAG TGATGACCAA CAACCCCTTG ATTCTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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EME1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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