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Test
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Test Description
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When to Request
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Solubility
Test
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Qualitative solubility test Free: Genscript offers you qualitative test
results for up to 3 solvents (default are Ultrapure water, 1 x DPBS(pH 7.1±0.1), DMSO) for free.
Quantitative solubility test New: Customized solvents are available
and specified quantitative test report (the highest gross peptide concentration can be achieved) will be
included.
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Recommended for:
hydrophobic peptides > 5 AA long, and
containing >50%:
- Tryptophan
- Isoleucine
- Leucine
- Phenylalanine
- Methionine
- Valine
- Tyrosine
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TFA Exchange
Service
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TFA is often used in the final cleavage of custom peptides from the solid
support. Hence,
traces of TFA may be left behind in the final peptide preparation, even after
purification.
Ion exchange chromatography is used to separate and quantify TFA.
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Recommended for:
peptides that will be used in:
- cellular assays
- API formulations
- manufactured products
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Counter
Ion
Quantification Analysis*
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Counter ions used in the synthesis process such
as acetate, hydrochloride, or
trifluoacetate
(TFA) may be present in your peptide preparation. Ion exchange chromatography is
used to
separate and quantify the various amounts of counter ions in the preparation.
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Bundle with TFA
Counter-ion Exchange for:
peptides that will be used in:
- cellular assays
- API formulations
- manufactured products
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Amino Acid Analysis*
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Peptide is hydrolyzed under acid conditions into free, individual amino
acid
residues.
Following derivation, reversed-phase liquid chromatography is used to separate
the amino
acid derivatives, followed by UV-detection and quantification.
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Request this test:
1). to determine the amino acid composition in
the peptide
preparation.
2). to determine the precise amount of the net peptide in the
preparation
to ensure experimental accuracy and consistency.§
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Peptide Content Analysis/Nitrogen Analysis*
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Peptide content analysis involves quantifying the amount of
elemental nitrogen in
the gross
peptide preparation.The amount of target peptide in the preparation is
calculated using
molar ratios.
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Request this test to determine the precise amount of the net peptide in
the gross
peptide preparation to ensure experimental accuracy and consistency.§
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Endotoxin Control &
Analysis
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Endotoxin, which can form throughout the process of peptide synthesis, can
decrease cell
viability or cause an immune response. Our endotoxin control service guarantees
endotoxin
levels in your custom peptide prep to be ≤ 0.01 EU/µg in order to improve your
experimental
results.
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Recommended for peptides used in:
- Cellular assays
- Immunotherapy
- Active pharmaceutical ingredients (APIs)
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Moisture Content Analysis*
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Even after lyophilization, water may still be present in a peptide preparation.
The amount of
water left behind depends on the hygroscopic nature of the peptide. Normally,
hydrophilic
peptides will retain the most water. Moisture content is determined by the Karl
Fischer
Titration method.
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Water % contributes to the gross weight of the peptide preparation
and may vary
from batch to
batch of the same peptide. It is important to know the exact amount of your
target peptide
in the gross peptide preparation to limit variability in results between batches
of the same
peptide.
Request this test to ensure precise dilution of
your peptide to
your desired stock concentration, which will limit batch to batch variability
between
different lots of the same peptide.
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pH Test
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Peptide is dissolved in water (additional solvents may be added in case of
hydrophobic custom
peptides) and a pH meter is used to determine the pH value of the peptide.
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Acids (e.g. TFA) used during peptide synthesis can form salts with
amino groups
on side
chains or the amino terminus of your peptide causing the pH of the preparation
to become
acidic. Low pH can interfere with assay results.
Request this
test to
ensure that your peptide preparation has your desired pH value.
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| NMR analysis |
NMR is mainly based on a physical
phenomenon, nuclear
magnetic resonance (NMR) of atomic nucleus either to determine the purity and
impurities of
peptide samples or to characterize impurities, through the carbon spectrum and
hydrogen
spectroscopy
NMR is usually used for microcosmic physicochemical properties
testing of
peptides, and the carbon or hydrogen spectrum is provided for the determination of
purity and
impurities in peptides.
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Request this test to determine the primary structure and the components of
impurities
in peptides.
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| LC-MS-analysis |
LC-MS is used for the components
analyses of
peptide samples, which can measure molecular weight online of all peptide
components.
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Request this test for real-time characterization of the peptide components
in peptide
samples, which is commonly applied to confirm the target HPLC peak or the analysis
of isomers.
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| LC MS/MS analysis |
LC MS/MS is employed for peptide sequence identification of individual peptides and for assessing peptide pooling coverage. It is also utilized in peptide degradation studies to quantify the main products and identify the degradation products.
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Recommended for:
- Peptide sequencing and primary structure determination;
- Evaluation of peptide pooling coverage;
- In vitro stability studies;
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| Stability test |
This test is used for the stability
test of
peptide samples under requested solvents. HPLC and MS characterization reports of
peptide
solution over a sustained period of time will be provided.
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Request this test to compare the stability of peptide in different
environments.
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| Peptide Sequencing |
N-terminal amino acid of peptide
samples is
degraded step-by-step by Edman degradation method, and then separated and identified
by HPLC.
The whole N-terminal amino acid sequence of peptide is tested.determined.
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Request this test to determine the primary structure of peptide samples.
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| Specific opticalrotation |
The plane-polarized light would be
rotated
through the peptide solution if the peptide containing asymmetric carbon atoms, and
the specific
optical rotation is the measured value of the optical rotation.
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Request this test to determine the optical activity and purity of peptides
especially
containing chiral amino acids.
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| HPLC-UV-fluorescence |
HPLC-UV-fluorescence detection is
beneficial for the
detection of peptides containing fluorescence adsorption group, which provides more
sensitive
and specific signals than single UV detector.
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Request this test for the purity or concentration determinations of peptide
samples
containing fluorescence adsorption groups(such as FITC, AMC, FAM, DABCYL, TMR and so
on)
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