Polr1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr1a
Entrez Gene ID	20019
Full Name	polymerase (RNA) I polypeptide A
Synonyms	194kDa,2900087K15Rik,3010014K16Rik,RPA194,Rpo1-4,mRPA1
General protein information	Preferred Names polymerase (RNA) I polypeptide A Names DNA-directed RNA polymerase I subunit RPA1 DNA-directed RNA polymerase I largest subunit DNA-directed RNA polymerase I subunit A RNA polymerase 1-4 (194 kDa subunit) RNA polymerase I 194 kDa subunit RNA polymerase I subunit A1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 C1\|6 32.21 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006505804.2	XP_006505867.1	DNA-directed RNA polymerase I subunit RPA1 isoform X1
NM_009088.3	NP_033114.3	DNA-directed RNA polymerase I subunit RPA1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
Macaca mulatta (Rhesus monkey)	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Neurospora crassa	NCU01638	XP_956006.2
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3

Gene Ontology
Bibliography

Related articles in PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.
Yamamoto K, Yamamoto M, Hanada K, Nogi Y, Matsuyama T, Muramatsu M
Molecular and cellular biology24(14)6338-49(2004 Jul)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

A kinetic framework for a mammalian RNA polymerase in vivo.
Dundr M, Hoffmann-Rohrer U, Hu Q, Grummt I, Rothblum LI, Phair RD, Misteli T
Science (New York, N.Y.)298(5598)1623-6(2002 Nov)

Molecular cloning and characterization of the cDNA encoding the largest subunit of mouse RNA polymerase I.
Seither P, Coy JF, Pouska A, Grummt I
Molecular & general genetics : MGG255(2)180-6(1997 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu37057	XM_006505804.2 Latest version!	Mus musculus polymerase (RNA) I polypeptide A (Polr1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu03497	NM_009088.3 Latest version!	Mus musculus polymerase (RNA) I polypeptide A (Polr1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr1a ( XM_006505804.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505867.1 Mus musculus polymerase (RNA) I polypeptide A (Polr1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37057
Clone ID Related Accession (Same CDS sequence)	XM_006505804.2
Accession Version	XM_006505804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase I subunit RPA1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006505804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCATCA ACGAGGATGG CAGTCGCACA GCCCTGAGCT CAGTGGATGC TGCGCAGCGG 
GAAGCTGTGG CCAAACAGCT CCTGACACCA GCCACAGGGG CTCCGAAGCC TCAGGGGACA 
AAAGTTGTGT GCCGGCATGT GAAAAATGGG GACATCCTCC TCCTGAACCG ACAGCCTACC 
TTGCACAGAC CCTCCATCCA GGCCCACCGT GCCCGCATTC TGCCTGAAGA AAAAGTCCTA 
CGCCTTCACT ATGCCAACTG CAAGGCCTAC AATGCTGACT TTGATGGAGA TGAGATGAAC 
GCCCACTTCC CCCAGAGTGA GTTGGGCCGA GCTGAAGCCT ATGTCCTGGC CTGCACTGAT 
CAGCAGTATC TTGTTCCCAA GGATGGCCAG CCATTGGCAG GACTGATCCA AGATCACATG 
GTTTCAGGTG CAAACATGAC CATTCGGGGA TGCTTTTTCA CCCGGGAACA GTACATGGAA 
CTGGTGTACC GAGGGCTCAC AGACAAAGTG GGACGCGTGA AGCTCTTTCC TCCCGCCATC 
CTGAAGCCCT TCCCACTGTG GACAGGAAAA CAGGTTGTGT CGACCCTCCT CATAAACATA 
ATTCCAGAGG ACTACGCCCC CCTGAACTTA TCTGGAAAGG CAAAAATTGG CAGCAAAGCC 
TGGGTGAAGG AAAAGCCACG GCCTATCCCT GACTTTGACC CGGACTCCAT GTGTGAGTCC 
CAGGTGATCA TCAGGGAAGG AGAGCTGCTC TGCGGAGTGC TGGACAAGGC ACACTATGGG 
AGCTCTGCCT ACGGCCTGGT CCACTGCTGC TATGAGATCT ATGGGGGTGA GACCAGCGGC 
AGGGTTCTCA CCTGCCTAGC CCGCCTCTTC ACTGCCTACC TCCAGCTCTA TAGAGGTTTC 
ACCTTGGGTG TAGAAGACAT TTTGGTTAAG CCAAATGCAG ATGTGGTGAG ACAGCGTATC 
ATTGAAGAAT CTACCCAGTG TGGACCCCAG GCTGTCAAGG CTGCATTGAG CCTGCCAGAA 
ACTGCATCAT GTGATGAAAT CCAAGGGAAA TGGCAGGATG CCCATCTGAG CAAAGACCAG 
AGGGATTTTA ACATGATTGA TATGAAGTTC AAGGAGGAAG TGAACCATTA CAGCAATGAG 
ATTAACAAGG CATGTATGCC TCTTGGCTTG CACAGACAGT TCCCCGAAAA CAACCTGCAG 
ATGATGGTGC AGTCAGGAGC CAAAGGGTCA ACCGTGAACA CGATGCAAAT CTCTTGCCTG 
CTGGGCCAAA TTGAATTGGA AGGTCGGAGA CCCCCACTGA TGGCGTCTGG CAAGTCACTG 
CCCTGCTTTG AGCCTTACGA GTTCACCCCC AGGGCTGGTG GCTTTGTCAC TGGAAGGTTT 
CTTACCGGTA TCAGGCCTCC TGAGTTCTTC TTCCACTGCA TGGCAGGACG AGAAGGCCTG 
GTGGACACTG CTGTCAAAAC CAGCCGTTCT GGCTATCTCC AAAGGTGTAT CATCAAGCAC 
CTGGAGGGGC TGGTCATCCA GTACGATCTG ACGGTCCGTG ACAGCGATGG CAGCGTGGTC 
CAGTTCCTGT ATGGGGAGGA CGGCCTGGAC ATCCCCAAGA CACAGTTCCT ACAGCCCAAG 
CAGTTCCCCT TTCTGGCCGG CAATTATGAG GTGATAATGA AATCCAAGCA TCTTCATGAA 
GTTTTATCCA GAGCAGATCC CCAGAAAGTG CTCGGCCACA TCAAAGCCAT TAAAAAATGG 
CATCACAAGC ATTCCGGTGC CCTGCTGAGG AAAGGCGCCT TCCTCAGTTT TTCCCAGAAG 
ATCCAGGCAG CTGTGAAAGC TTTGAACCTA AAGGGCAGTA TCCAGAATGG CCGCAGCCCC 
GAGACCCAGC AGATGTTACA GATGTGGTAT GACTTGGATG AAGAGAGCCG ATGGAAATAC 
CAGAAGAGGG CTGCCCCTTG TCCCGACCCT AGCCTTTCTG TGTGGCGCCC AGACATCTAC 
TTTGCATCTG TGTCTGAAAC ATTTGAAAAG AAGATTGATG ACTTCAGTCA GGAATGGGCA 
GCTCAAGCAG AGAGGAGTTA CAAGAAATCA GAGCTTTCCC TCGACAGGCT GAGAACCTTA 
CTGCAGCTGA AGTGGCAGCG CTCACTGTGT GACCCAGGCG AGGCTGTTGG CCTCCTGGCT 
GCCCAGAGCA TCGGAGAGCC CTCCACACAG ATGACACTGA ACACCTTCCA CTTTGCAGGC 
AGAGGCGAGA TGAACGTCAC CCTGGGCATT CCAAGATTGA GGGAGATTCT CATGGTGGCC 
AGTGCCAACA TCAAAACGCC CATGATGAGT GTGCCTGTGT TCGACACCAA GAAAGCATTG 
AAGAAAGTGA AGAGTCTAAA GAAGAGGCTC ACCAGAGTGT GCCTGGGGGA GGTATTGCAG 
AAAGTTGACA TCCAGGAGTC CTTCTGTATG GGAGAAAAAC GGAATAAGTT CCAGGTGTAT 
GAGCTCCGGT TTCAGTTCCT GCCACATGCA TATTACCAGC AGGAGAAGTG CCTGAGACCC 
GAGGACATCT TGCACTTCAT GGAAACCAGA TTCTTTAAGC TACTAATGGA AGCTATCAAA 
AAGAAGAAAA ACAAAGCATC AGCCTTCAGA AATGTGAATT CGAGGAGAGC TACACAGAAG 
GACCTCAATG ACACTGAGGA CTCGGGGAGG AGTCAGAGAG AGGAGGAAAG GGATGAGGAA 
GAGGAGGGGA ACATTGTGGA TGCTGAAGCT GAGGAGGGCG ATGCAGACGC TTCTGACACC 
AAACGCAAGG AGAAGCAAGA AGAAGAGGTT GACTATGAGA GTGAGGAAGA AGGAGAGGAG 
GAAGAGGAGG AGGAGGTACA AGAAGAAGGG AACATCAAAG GCGACGGTGT CCACCAGGGC 
CATGAGCCAG ATGAGGAAGA ACATTTAGGC CTCGAGGAGG AGGAGTCATC CCAGAAGCCT 
CCCCGCCGCC ACTCCAGGCC TCAGGGAGCT GAGGCCATAA AACGCCGGAT CCAGGCTGTA 
CGGGAGTCCT ACTCGTTCAT CGAGGACTAC CAGTATGACA CAGAGGAGAG CCTGTGGTGT 
CAGGTGACTG TGAAGCTCCC ACTGATGAAG ATTAATTTTG ACATGAGCTC CTTGGTTGTG 
TCCTTGGCCC ACAAAGCCAT TGTCTATACG ACCAAGGGCA TCACTCGGTG CCTCCTGAAC 
GAAACGACCA ACAGTAAGAA TGAGAAGGAG CTTGTACTGA ACACGGAGGG AATCAACCTT 
CCCGAGCTGT TCAAGTACTC CGAGATACTG GACCTGCGCC GCCTCTACTC CAATGACATC 
CACGCCATGG CCAATACTTA TGGCATCGAG GCTGCGTTGC GGGTGATAGA GAAGGAGATT 
AAAGATGTGT TTGCTGTGTA TGGCATTGCA GTTGATCCTC GCCATCTCTC CCTGGTGGCT 
GATTACATGT GTTTTGAGGG GGTTTATAAG CCACTCAATC GCTTTGGGAT CCAGTCAAGC 
TCCTCCCCTT TGCAGCAGAT GACATTTGAA ACCAGCTTCC AGTTTCTGAA ACAGGCCACC 
ATGATGGGAT CCCATGATGA ACTCAAGTCA CCTTCCGCCT GCCTCGTGGT GGGAAAGGTT 
GTCAAAGGCG GGACAGGCCT GTTTGAGCTC AAGCAGCCCC TAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505867.1
CDS	265..3912
Translation	MVINEDGSRTALSSVDAAQREAVAKQLLTPATGAPKPQGTKVVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYAPLNLSGKAKIGSKAWVKEKPRPIPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVVRQRIIEESTQCGPQAVKAALSLPETASCDEIQGKWQDAHLSKDQRDFNMIDMKFKEEVNHYSNEINKACMPLGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLAGNYEVIMKSKHLHEVLSRADPQKVLGHIKAIKKWHHKHSGALLRKGAFLSFSQKIQAAVKALNLKGSIQNGRSPETQQMLQMWYDLDEESRWKYQKRAAPCPDPSLSVWRPDIYFASVSETFEKKIDDFSQEWAAQAERSYKKSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFDTKKALKKVKSLKKRLTRVCLGEVLQKVDIQESFCMGEKRNKFQVYELRFQFLPHAYYQQEKCLRPEDILHFMETRFFKLLMEAIKKKKNKASAFRNVNSRRATQKDLNDTEDSGRSQREEERDEEEEGNIVDAEAEEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEEVQEEGNIKGDGVHQGHEPDEEEHLGLEEEESSQKPPRRHSRPQGAEAIKRRIQAVRESYSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHKAIVYTTKGITRCLLNETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPSACLVVGKVVKGGTGLFELKQPLR

Target ORF information:

RefSeq Version	XM_006505804.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) I polypeptide A (Polr1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505804.2

ORF Insert Sequence:

ATGGTCATCA ACGAGGATGG CAGTCGCACA GCCCTGAGCT CAGTGGATGC TGCGCAGCGG 
GAAGCTGTGG CCAAACAGCT CCTGACACCA GCCACAGGGG CTCCGAAGCC TCAGGGGACA 
AAAGTTGTGT GCCGGCATGT GAAAAATGGG GACATCCTCC TCCTGAACCG ACAGCCTACC 
TTGCACAGAC CCTCCATCCA GGCCCACCGT GCCCGCATTC TGCCTGAAGA AAAAGTCCTA 
CGCCTTCACT ATGCCAACTG CAAGGCCTAC AATGCTGACT TTGATGGAGA TGAGATGAAC 
GCCCACTTCC CCCAGAGTGA GTTGGGCCGA GCTGAAGCCT ATGTCCTGGC CTGCACTGAT 
CAGCAGTATC TTGTTCCCAA GGATGGCCAG CCATTGGCAG GACTGATCCA AGATCACATG 
GTTTCAGGTG CAAACATGAC CATTCGGGGA TGCTTTTTCA CCCGGGAACA GTACATGGAA 
CTGGTGTACC GAGGGCTCAC AGACAAAGTG GGACGCGTGA AGCTCTTTCC TCCCGCCATC 
CTGAAGCCCT TCCCACTGTG GACAGGAAAA CAGGTTGTGT CGACCCTCCT CATAAACATA 
ATTCCAGAGG ACTACGCCCC CCTGAACTTA TCTGGAAAGG CAAAAATTGG CAGCAAAGCC 
TGGGTGAAGG AAAAGCCACG GCCTATCCCT GACTTTGACC CGGACTCCAT GTGTGAGTCC 
CAGGTGATCA TCAGGGAAGG AGAGCTGCTC TGCGGAGTGC TGGACAAGGC ACACTATGGG 
AGCTCTGCCT ACGGCCTGGT CCACTGCTGC TATGAGATCT ATGGGGGTGA GACCAGCGGC 
AGGGTTCTCA CCTGCCTAGC CCGCCTCTTC ACTGCCTACC TCCAGCTCTA TAGAGGTTTC 
ACCTTGGGTG TAGAAGACAT TTTGGTTAAG CCAAATGCAG ATGTGGTGAG ACAGCGTATC 
ATTGAAGAAT CTACCCAGTG TGGACCCCAG GCTGTCAAGG CTGCATTGAG CCTGCCAGAA 
ACTGCATCAT GTGATGAAAT CCAAGGGAAA TGGCAGGATG CCCATCTGAG CAAAGACCAG 
AGGGATTTTA ACATGATTGA TATGAAGTTC AAGGAGGAAG TGAACCATTA CAGCAATGAG 
ATTAACAAGG CATGTATGCC TCTTGGCTTG CACAGACAGT TCCCCGAAAA CAACCTGCAG 
ATGATGGTGC AGTCAGGAGC CAAAGGGTCA ACCGTGAACA CGATGCAAAT CTCTTGCCTG 
CTGGGCCAAA TTGAATTGGA AGGTCGGAGA CCCCCACTGA TGGCGTCTGG CAAGTCACTG 
CCCTGCTTTG AGCCTTACGA GTTCACCCCC AGGGCTGGTG GCTTTGTCAC TGGAAGGTTT 
CTTACCGGTA TCAGGCCTCC TGAGTTCTTC TTCCACTGCA TGGCAGGACG AGAAGGCCTG 
GTGGACACTG CTGTCAAAAC CAGCCGTTCT GGCTATCTCC AAAGGTGTAT CATCAAGCAC 
CTGGAGGGGC TGGTCATCCA GTACGATCTG ACGGTCCGTG ACAGCGATGG CAGCGTGGTC 
CAGTTCCTGT ATGGGGAGGA CGGCCTGGAC ATCCCCAAGA CACAGTTCCT ACAGCCCAAG 
CAGTTCCCCT TTCTGGCCGG CAATTATGAG GTGATAATGA AATCCAAGCA TCTTCATGAA 
GTTTTATCCA GAGCAGATCC CCAGAAAGTG CTCGGCCACA TCAAAGCCAT TAAAAAATGG 
CATCACAAGC ATTCCGGTGC CCTGCTGAGG AAAGGCGCCT TCCTCAGTTT TTCCCAGAAG 
ATCCAGGCAG CTGTGAAAGC TTTGAACCTA AAGGGCAGTA TCCAGAATGG CCGCAGCCCC 
GAGACCCAGC AGATGTTACA GATGTGGTAT GACTTGGATG AAGAGAGCCG ATGGAAATAC 
CAGAAGAGGG CTGCCCCTTG TCCCGACCCT AGCCTTTCTG TGTGGCGCCC AGACATCTAC 
TTTGCATCTG TGTCTGAAAC ATTTGAAAAG AAGATTGATG ACTTCAGTCA GGAATGGGCA 
GCTCAAGCAG AGAGGAGTTA CAAGAAATCA GAGCTTTCCC TCGACAGGCT GAGAACCTTA 
CTGCAGCTGA AGTGGCAGCG CTCACTGTGT GACCCAGGCG AGGCTGTTGG CCTCCTGGCT 
GCCCAGAGCA TCGGAGAGCC CTCCACACAG ATGACACTGA ACACCTTCCA CTTTGCAGGC 
AGAGGCGAGA TGAACGTCAC CCTGGGCATT CCAAGATTGA GGGAGATTCT CATGGTGGCC 
AGTGCCAACA TCAAAACGCC CATGATGAGT GTGCCTGTGT TCGACACCAA GAAAGCATTG 
AAGAAAGTGA AGAGTCTAAA GAAGAGGCTC ACCAGAGTGT GCCTGGGGGA GGTATTGCAG 
AAAGTTGACA TCCAGGAGTC CTTCTGTATG GGAGAAAAAC GGAATAAGTT CCAGGTGTAT 
GAGCTCCGGT TTCAGTTCCT GCCACATGCA TATTACCAGC AGGAGAAGTG CCTGAGACCC 
GAGGACATCT TGCACTTCAT GGAAACCAGA TTCTTTAAGC TACTAATGGA AGCTATCAAA 
AAGAAGAAAA ACAAAGCATC AGCCTTCAGA AATGTGAATT CGAGGAGAGC TACACAGAAG 
GACCTCAATG ACACTGAGGA CTCGGGGAGG AGTCAGAGAG AGGAGGAAAG GGATGAGGAA 
GAGGAGGGGA ACATTGTGGA TGCTGAAGCT GAGGAGGGCG ATGCAGACGC TTCTGACACC 
AAACGCAAGG AGAAGCAAGA AGAAGAGGTT GACTATGAGA GTGAGGAAGA AGGAGAGGAG 
GAAGAGGAGG AGGAGGTACA AGAAGAAGGG AACATCAAAG GCGACGGTGT CCACCAGGGC 
CATGAGCCAG ATGAGGAAGA ACATTTAGGC CTCGAGGAGG AGGAGTCATC CCAGAAGCCT 
CCCCGCCGCC ACTCCAGGCC TCAGGGAGCT GAGGCCATAA AACGCCGGAT CCAGGCTGTA 
CGGGAGTCCT ACTCGTTCAT CGAGGACTAC CAGTATGACA CAGAGGAGAG CCTGTGGTGT 
CAGGTGACTG TGAAGCTCCC ACTGATGAAG ATTAATTTTG ACATGAGCTC CTTGGTTGTG 
TCCTTGGCCC ACAAAGCCAT TGTCTATACG ACCAAGGGCA TCACTCGGTG CCTCCTGAAC 
GAAACGACCA ACAGTAAGAA TGAGAAGGAG CTTGTACTGA ACACGGAGGG AATCAACCTT 
CCCGAGCTGT TCAAGTACTC CGAGATACTG GACCTGCGCC GCCTCTACTC CAATGACATC 
CACGCCATGG CCAATACTTA TGGCATCGAG GCTGCGTTGC GGGTGATAGA GAAGGAGATT 
AAAGATGTGT TTGCTGTGTA TGGCATTGCA GTTGATCCTC GCCATCTCTC CCTGGTGGCT 
GATTACATGT GTTTTGAGGG GGTTTATAAG CCACTCAATC GCTTTGGGAT CCAGTCAAGC 
TCCTCCCCTT TGCAGCAGAT GACATTTGAA ACCAGCTTCC AGTTTCTGAA ACAGGCCACC 
ATGATGGGAT CCCATGATGA ACTCAAGTCA CCTTCCGCCT GCCTCGTGGT GGGAAAGGTT 
GTCAAAGGCG GGACAGGCCT GTTTGAGCTC AAGCAGCCCC TAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr1a ( NM_009088.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033114.3 Mus musculus polymerase (RNA) I polypeptide A (Polr1a), mRNA.

Price & Availability↑

CloneID	OMu03497
Clone ID Related Accession (Same CDS sequence)	NM_009088.3
Accession Version	NM_009088.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK160970.1, AK147379.1 and CJ260853.1. On Sep 1, 2009 this sequence version replaced NM_009088.2. ##Evidence-Data-START## Transcript exon combination :: BC053744.1, AK147379.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGCCT CCAAGCACAC ACCATGGAGG CGGCTTCAGG GCATTTCCTT CGGAATGTAT 
TCGGCCGAAG AGCTCAAGAA GTTAAGTGTT AAATCCATTA CAAATCCTCG ATACGTGGAC 
TACCTGGGAA ACCCTTCAGC AAATGGCCTG TATGATTTGG CTTTGGGTCC TGCAGATTCC 
AAAGAAGTAT GCGCTACCTG CGTGCAGGAC TTCAACAACT GCTCTGGACA CCTGGGCCAC 
ATTGATCTCC CGCTCACAGT CTACAACCCT TTCCTCTTTG ATAAACTCTA CCTGCTGCTT 
CGAGGCTCCT GTTTGAGTTG CCACATGCTG ACTTGTCCTC GGGCTGCAAT TTACCTGTTG 
ATCTCCCAGC TGAGGGTCCT GGAAGTTGGA GCCCTGCAAG CAGTCTACGA GCTTGAGCGA 
ATTCTCAGCA GGTTTCTGGA AGAAACTGGA GATCCTTCCG CCTTCGAAAT TCAAGAAGAG 
TTAGAAGAAT ACACAAGCAA AATTCTGCAG AACAACCTCT TGGGGTCCCA GGGCACACAT 
GTGAAAAATG TGTGCGAGAG CAGAAGCAAA CTTGTCGCTC AGTTCTGGAA GACACACATG 
GCTGCGAAGC AGTGTCCACA CTGCAAGACG GGACGGTCGG TGGTTCGAAA GGAGCACAAT 
AGTAAACTAA TCATCACATA CCCAGCCACG GTGCACAAGA AATCAGACCA GGAGGGCACA 
GAACTCCCAG AAGGAGTGCC GGAAGCTCCA GGAATTGACA AGGCTCAGAT GGGGAAACGA 
GGGTACCTGA CCCCAAGCAG CGCCCAAGAA CACCTTTTCG CCATTTGGAA GAATGAAGGA 
TTCTTTCTGA ATTATCTTTT TTCGGGATTG GATGATATTG GCCCAGAGTC CAGTTTCAAC 
CCCAGTATGT TCTTTCTAGA CTTCATAGTC GTACCGCCAT CCAGGTACCG TCCCGTCAAT 
CGCCTGGGTG ACCAGATGTT CACTAATGGC CAGACAGTAA ACTTGCAAGC TGTCATGAAG 
GATGCAGTTT TGATCCGGAA ACTTCTGGCG TTGATGGCCC AAGAACAGAA ACTGCCCTGT 
GAGATGACAG AACTCACCAT AGACAAGGAA AATGACTCCT CAGTGGCTAT CGACCGATCC 
TTCCTGGGTT TGCTTCCAGG CCCGTCTCTC ACAGATAAAC TTTACAACAT TTGGATTCGC 
CTTCAGAGCC ACGTCAATAT TGTGTTTGAC AGTGAGATGG ACAAATTGAT GTTGGAAAAG 
TACCCTGGCA TAAGACAGAT CCTAGAGAAG AAGGAAGGCC TGTTCCGGAA GCACATGATG 
GGAAAGCGAG TTGACTACGC AGCCCGCTCG GTCATCTGCC CAGACATGTA CATCAACACC 
AATGAGATCG GAATCCCCAT GGTATTTGCC ACAAAACTGA CTTACCCTCA GCCAGTTACC 
CCCTGGAACG TGCAGGAACT GAGACAGGCA GTCATCAATG GACCCAATGT GCACCCAGGA 
GCCTCCATGG TCATCAACGA GGATGGCAGT CGCACAGCCC TGAGCTCAGT GGATGCTGCG 
CAGCGGGAAG CTGTGGCCAA ACAGCTCCTG ACACCAGCCA CAGGGGCTCC GAAGCCTCAG 
GGGACAAAAG TTGTGTGCCG GCATGTGAAA AATGGGGACA TCCTCCTCCT GAACCGACAG 
CCTACCTTGC ACAGACCCTC CATCCAGGCC CACCGTGCCC GCATTCTGCC TGAAGAAAAA 
GTCCTACGCC TTCACTATGC CAACTGCAAG GCCTACAATG CTGACTTTGA TGGAGATGAG 
ATGAACGCCC ACTTCCCCCA GAGTGAGTTG GGCCGAGCTG AAGCCTATGT CCTGGCCTGC 
ACTGATCAGC AGTATCTTGT TCCCAAGGAT GGCCAGCCAT TGGCAGGACT GATCCAAGAT 
CACATGGTTT CAGGTGCAAA CATGACCATT CGGGGATGCT TTTTCACCCG GGAACAGTAC 
ATGGAACTGG TGTACCGAGG GCTCACAGAC AAAGTGGGAC GCGTGAAGCT CTTTCCTCCC 
GCCATCCTGA AGCCCTTCCC ACTGTGGACA GGAAAACAGG TTGTGTCGAC CCTCCTCATA 
AACATAATTC CAGAGGACTA CGCCCCCCTG AACTTATCTG GAAAGGCAAA AATTGGCAGC 
AAAGCCTGGG TGAAGGAAAA GCCACGGCCT ATCCCTGACT TTGACCCGGA CTCCATGTGT 
GAGTCCCAGG TGATCATCAG GGAAGGAGAG CTGCTCTGCG GAGTGCTGGA CAAGGCACAC 
TATGGGAGCT CTGCCTACGG CCTGGTCCAC TGCTGCTATG AGATCTATGG GGGTGAGACC 
AGCGGCAGGG TTCTCACCTG CCTAGCCCGC CTCTTCACTG CCTACCTCCA GCTCTATAGA 
GGTTTCACCT TGGGTGTAGA AGACATTTTG GTTAAGCCAA ATGCAGATGT GGTGAGACAG 
CGTATCATTG AAGAATCTAC CCAGTGTGGA CCCCAGGCTG TCAAGGCTGC ATTGAGCCTG 
CCAGAAACTG CATCATGTGA TGAAATCCAA GGGAAATGGC AGGATGCCCA TCTGAGCAAA 
GACCAGAGGG ATTTTAACAT GATTGATATG AAGTTCAAGG AGGAAGTGAA CCATTACAGC 
AATGAGATTA ACAAGGCATG TATGCCTCTT GGCTTGCACA GACAGTTCCC CGAAAACAAC 
CTGCAGATGA TGGTGCAGTC AGGAGCCAAA GGGTCAACCG TGAACACGAT GCAAATCTCT 
TGCCTGCTGG GCCAAATTGA ATTGGAAGGT CGGAGACCCC CACTGATGGC GTCTGGCAAG 
TCACTGCCCT GCTTTGAGCC TTACGAGTTC ACCCCCAGGG CTGGTGGCTT TGTCACTGGA 
AGGTTTCTTA CCGGTATCAG GCCTCCTGAG TTCTTCTTCC ACTGCATGGC AGGACGAGAA 
GGCCTGGTGG ACACTGCTGT CAAAACCAGC CGTTCTGGCT ATCTCCAAAG GTGTATCATC 
AAGCACCTGG AGGGGCTGGT CATCCAGTAC GATCTGACGG TCCGTGACAG CGATGGCAGC 
GTGGTCCAGT TCCTGTATGG GGAGGACGGC CTGGACATCC CCAAGACACA GTTCCTACAG 
CCCAAGCAGT TCCCCTTTCT GGCCGGCAAT TATGAGGTGA TAATGAAATC CAAGCATCTT 
CATGAAGTTT TATCCAGAGC AGATCCCCAG AAAGTGCTCG GCCACATCAA AGCCATTAAA 
AAATGGCATC ACAAGCATTC CGGTGCCCTG CTGAGGAAAG GCGCCTTCCT CAGTTTTTCC 
CAGAAGATCC AGGCAGCTGT GAAAGCTTTG AACCTAAAGG GCAGTATCCA GAATGGCCGC 
AGCCCCGAGA CCCAGCAGAT GTTACAGATG TGGTATGACT TGGATGAAGA GAGCCGATGG 
AAATACCAGA AGAGGGCTGC CCCTTGTCCC GACCCTAGCC TTTCTGTGTG GCGCCCAGAC 
ATCTACTTTG CATCTGTGTC TGAAACATTT GAAAAGAAGA TTGATGACTT CAGTCAGGAA 
TGGGCAGCTC AAGCAGAGAG GAGTTACAAG AAATCAGAGC TTTCCCTCGA CAGGCTGAGA 
ACCTTACTGC AGCTGAAGTG GCAGCGCTCA CTGTGTGACC CAGGCGAGGC TGTTGGCCTC 
CTGGCTGCCC AGAGCATCGG AGAGCCCTCC ACACAGATGA CACTGAACAC CTTCCACTTT 
GCAGGCAGAG GCGAGATGAA CGTCACCCTG GGCATTCCAA GATTGAGGGA GATTCTCATG 
GTGGCCAGTG CCAACATCAA AACGCCCATG ATGAGTGTGC CTGTGTTCGA CACCAAGAAA 
GCATTGAAGA AAGTGAAGAG TCTAAAGAAG AGGCTCACCA GAGTGTGCCT GGGGGAGGTA 
TTGCAGAAAG TTGACATCCA GGAGTCCTTC TGTATGGGAG AAAAACGGAA TAAGTTCCAG 
GTGTATGAGC TCCGGTTTCA GTTCCTGCCA CATGCATATT ACCAGCAGGA GAAGTGCCTG 
AGACCCGAGG ACATCTTGCA CTTCATGGAA ACCAGATTCT TTAAGCTACT AATGGAAGCT 
ATCAAAAAGA AGAAAAACAA AGCATCAGCC TTCAGAAATG TGAATTCGAG GAGAGCTACA 
CAGAAGGACC TCAATGACAC TGAGGACTCG GGGAGGAGTC AGAGAGAGGA GGAAAGGGAT 
GAGGAAGAGG AGGGGAACAT TGTGGATGCT GAAGCTGAGG AGGGCGATGC AGACGCTTCT 
GACACCAAAC GCAAGGAGAA GCAAGAAGAA GAGGTTGACT ATGAGAGTGA GGAAGAAGGA 
GAGGAGGAAG AGGAGGAGGA GGTACAAGAA GAAGGGAACA TCAAAGGCGA CGGTGTCCAC 
CAGGGCCATG AGCCAGATGA GGAAGAACAT TTAGGCCTCG AGGAGGAGGA GTCATCCCAG 
AAGCCTCCCC GCCGCCACTC CAGGCCTCAG GGAGCTGAGG CCATAAAACG CCGGATCCAG 
GCTGTACGGG AGTCCTACTC GTTCATCGAG GACTACCAGT ATGACACAGA GGAGAGCCTG 
TGGTGTCAGG TGACTGTGAA GCTCCCACTG ATGAAGATTA ATTTTGACAT GAGCTCCTTG 
GTTGTGTCCT TGGCCCACAA AGCCATTGTC TATACGACCA AGGGCATCAC TCGGTGCCTC 
CTGAACGAAA CGACCAACAG TAAGAATGAG AAGGAGCTTG TACTGAACAC GGAGGGAATC 
AACCTTCCCG AGCTGTTCAA GTACTCCGAG ATACTGGACC TGCGCCGCCT CTACTCCAAT 
GACATCCACG CCATGGCCAA TACTTATGGC ATCGAGGCTG CGTTGCGGGT GATAGAGAAG 
GAGATTAAAG ATGTGTTTGC TGTGTATGGC ATTGCAGTTG ATCCTCGCCA TCTCTCCCTG 
GTGGCTGATT ACATGTGTTT TGAGGGGGTT TATAAGCCAC TCAATCGCTT TGGGATCCAG 
TCAAGCTCCT CCCCTTTGCA GCAGATGACA TTTGAAACCA GCTTCCAGTT TCTGAAACAG 
GCCACCATGA TGGGATCCCA TGATGAACTC AAGTCACCTT CCGCCTGCCT CGTGGTGGGA 
AAGGTTGTCA AAGGCGGGAC AGGCCTGTTT GAGCTCAAGC AGCCCCTAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033114.3
CDS	111..5264
Translation	MLASKHTPWRRLQGISFGMYSAEELKKLSVKSITNPRYVDYLGNPSANGLYDLALGPADSKEVCATCVQDFNNCSGHLGHIDLPLTVYNPFLFDKLYLLLRGSCLSCHMLTCPRAAIYLLISQLRVLEVGALQAVYELERILSRFLEETGDPSAFEIQEELEEYTSKILQNNLLGSQGTHVKNVCESRSKLVAQFWKTHMAAKQCPHCKTGRSVVRKEHNSKLIITYPATVHKKSDQEGTELPEGVPEAPGIDKAQMGKRGYLTPSSAQEHLFAIWKNEGFFLNYLFSGLDDIGPESSFNPSMFFLDFIVVPPSRYRPVNRLGDQMFTNGQTVNLQAVMKDAVLIRKLLALMAQEQKLPCEMTELTIDKENDSSVAIDRSFLGLLPGPSLTDKLYNIWIRLQSHVNIVFDSEMDKLMLEKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSSVDAAQREAVAKQLLTPATGAPKPQGTKVVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYAPLNLSGKAKIGSKAWVKEKPRPIPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVVRQRIIEESTQCGPQAVKAALSLPETASCDEIQGKWQDAHLSKDQRDFNMIDMKFKEEVNHYSNEINKACMPLGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLAGNYEVIMKSKHLHEVLSRADPQKVLGHIKAIKKWHHKHSGALLRKGAFLSFSQKIQAAVKALNLKGSIQNGRSPETQQMLQMWYDLDEESRWKYQKRAAPCPDPSLSVWRPDIYFASVSETFEKKIDDFSQEWAAQAERSYKKSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFDTKKALKKVKSLKKRLTRVCLGEVLQKVDIQESFCMGEKRNKFQVYELRFQFLPHAYYQQEKCLRPEDILHFMETRFFKLLMEAIKKKKNKASAFRNVNSRRATQKDLNDTEDSGRSQREEERDEEEEGNIVDAEAEEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEEVQEEGNIKGDGVHQGHEPDEEEHLGLEEEESSQKPPRRHSRPQGAEAIKRRIQAVRESYSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHKAIVYTTKGITRCLLNETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPSACLVVGKVVKGGTGLFELKQPLR

Target ORF information:

RefSeq Version	NM_009088.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) I polypeptide A (Polr1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009088.3

ORF Insert Sequence:

ATGCTGGCCT CCAAGCACAC ACCATGGAGG CGGCTTCAGG GCATTTCCTT CGGAATGTAT 
TCGGCCGAAG AGCTCAAGAA GTTAAGTGTT AAATCCATTA CAAATCCTCG ATACGTGGAC 
TACCTGGGAA ACCCTTCAGC AAATGGCCTG TATGATTTGG CTTTGGGTCC TGCAGATTCC 
AAAGAAGTAT GCGCTACCTG CGTGCAGGAC TTCAACAACT GCTCTGGACA CCTGGGCCAC 
ATTGATCTCC CGCTCACAGT CTACAACCCT TTCCTCTTTG ATAAACTCTA CCTGCTGCTT 
CGAGGCTCCT GTTTGAGTTG CCACATGCTG ACTTGTCCTC GGGCTGCAAT TTACCTGTTG 
ATCTCCCAGC TGAGGGTCCT GGAAGTTGGA GCCCTGCAAG CAGTCTACGA GCTTGAGCGA 
ATTCTCAGCA GGTTTCTGGA AGAAACTGGA GATCCTTCCG CCTTCGAAAT TCAAGAAGAG 
TTAGAAGAAT ACACAAGCAA AATTCTGCAG AACAACCTCT TGGGGTCCCA GGGCACACAT 
GTGAAAAATG TGTGCGAGAG CAGAAGCAAA CTTGTCGCTC AGTTCTGGAA GACACACATG 
GCTGCGAAGC AGTGTCCACA CTGCAAGACG GGACGGTCGG TGGTTCGAAA GGAGCACAAT 
AGTAAACTAA TCATCACATA CCCAGCCACG GTGCACAAGA AATCAGACCA GGAGGGCACA 
GAACTCCCAG AAGGAGTGCC GGAAGCTCCA GGAATTGACA AGGCTCAGAT GGGGAAACGA 
GGGTACCTGA CCCCAAGCAG CGCCCAAGAA CACCTTTTCG CCATTTGGAA GAATGAAGGA 
TTCTTTCTGA ATTATCTTTT TTCGGGATTG GATGATATTG GCCCAGAGTC CAGTTTCAAC 
CCCAGTATGT TCTTTCTAGA CTTCATAGTC GTACCGCCAT CCAGGTACCG TCCCGTCAAT 
CGCCTGGGTG ACCAGATGTT CACTAATGGC CAGACAGTAA ACTTGCAAGC TGTCATGAAG 
GATGCAGTTT TGATCCGGAA ACTTCTGGCG TTGATGGCCC AAGAACAGAA ACTGCCCTGT 
GAGATGACAG AACTCACCAT AGACAAGGAA AATGACTCCT CAGTGGCTAT CGACCGATCC 
TTCCTGGGTT TGCTTCCAGG CCCGTCTCTC ACAGATAAAC TTTACAACAT TTGGATTCGC 
CTTCAGAGCC ACGTCAATAT TGTGTTTGAC AGTGAGATGG ACAAATTGAT GTTGGAAAAG 
TACCCTGGCA TAAGACAGAT CCTAGAGAAG AAGGAAGGCC TGTTCCGGAA GCACATGATG 
GGAAAGCGAG TTGACTACGC AGCCCGCTCG GTCATCTGCC CAGACATGTA CATCAACACC 
AATGAGATCG GAATCCCCAT GGTATTTGCC ACAAAACTGA CTTACCCTCA GCCAGTTACC 
CCCTGGAACG TGCAGGAACT GAGACAGGCA GTCATCAATG GACCCAATGT GCACCCAGGA 
GCCTCCATGG TCATCAACGA GGATGGCAGT CGCACAGCCC TGAGCTCAGT GGATGCTGCG 
CAGCGGGAAG CTGTGGCCAA ACAGCTCCTG ACACCAGCCA CAGGGGCTCC GAAGCCTCAG 
GGGACAAAAG TTGTGTGCCG GCATGTGAAA AATGGGGACA TCCTCCTCCT GAACCGACAG 
CCTACCTTGC ACAGACCCTC CATCCAGGCC CACCGTGCCC GCATTCTGCC TGAAGAAAAA 
GTCCTACGCC TTCACTATGC CAACTGCAAG GCCTACAATG CTGACTTTGA TGGAGATGAG 
ATGAACGCCC ACTTCCCCCA GAGTGAGTTG GGCCGAGCTG AAGCCTATGT CCTGGCCTGC 
ACTGATCAGC AGTATCTTGT TCCCAAGGAT GGCCAGCCAT TGGCAGGACT GATCCAAGAT 
CACATGGTTT CAGGTGCAAA CATGACCATT CGGGGATGCT TTTTCACCCG GGAACAGTAC 
ATGGAACTGG TGTACCGAGG GCTCACAGAC AAAGTGGGAC GCGTGAAGCT CTTTCCTCCC 
GCCATCCTGA AGCCCTTCCC ACTGTGGACA GGAAAACAGG TTGTGTCGAC CCTCCTCATA 
AACATAATTC CAGAGGACTA CGCCCCCCTG AACTTATCTG GAAAGGCAAA AATTGGCAGC 
AAAGCCTGGG TGAAGGAAAA GCCACGGCCT ATCCCTGACT TTGACCCGGA CTCCATGTGT 
GAGTCCCAGG TGATCATCAG GGAAGGAGAG CTGCTCTGCG GAGTGCTGGA CAAGGCACAC 
TATGGGAGCT CTGCCTACGG CCTGGTCCAC TGCTGCTATG AGATCTATGG GGGTGAGACC 
AGCGGCAGGG TTCTCACCTG CCTAGCCCGC CTCTTCACTG CCTACCTCCA GCTCTATAGA 
GGTTTCACCT TGGGTGTAGA AGACATTTTG GTTAAGCCAA ATGCAGATGT GGTGAGACAG 
CGTATCATTG AAGAATCTAC CCAGTGTGGA CCCCAGGCTG TCAAGGCTGC ATTGAGCCTG 
CCAGAAACTG CATCATGTGA TGAAATCCAA GGGAAATGGC AGGATGCCCA TCTGAGCAAA 
GACCAGAGGG ATTTTAACAT GATTGATATG AAGTTCAAGG AGGAAGTGAA CCATTACAGC 
AATGAGATTA ACAAGGCATG TATGCCTCTT GGCTTGCACA GACAGTTCCC CGAAAACAAC 
CTGCAGATGA TGGTGCAGTC AGGAGCCAAA GGGTCAACCG TGAACACGAT GCAAATCTCT 
TGCCTGCTGG GCCAAATTGA ATTGGAAGGT CGGAGACCCC CACTGATGGC GTCTGGCAAG 
TCACTGCCCT GCTTTGAGCC TTACGAGTTC ACCCCCAGGG CTGGTGGCTT TGTCACTGGA 
AGGTTTCTTA CCGGTATCAG GCCTCCTGAG TTCTTCTTCC ACTGCATGGC AGGACGAGAA 
GGCCTGGTGG ACACTGCTGT CAAAACCAGC CGTTCTGGCT ATCTCCAAAG GTGTATCATC 
AAGCACCTGG AGGGGCTGGT CATCCAGTAC GATCTGACGG TCCGTGACAG CGATGGCAGC 
GTGGTCCAGT TCCTGTATGG GGAGGACGGC CTGGACATCC CCAAGACACA GTTCCTACAG 
CCCAAGCAGT TCCCCTTTCT GGCCGGCAAT TATGAGGTGA TAATGAAATC CAAGCATCTT 
CATGAAGTTT TATCCAGAGC AGATCCCCAG AAAGTGCTCG GCCACATCAA AGCCATTAAA 
AAATGGCATC ACAAGCATTC CGGTGCCCTG CTGAGGAAAG GCGCCTTCCT CAGTTTTTCC 
CAGAAGATCC AGGCAGCTGT GAAAGCTTTG AACCTAAAGG GCAGTATCCA GAATGGCCGC 
AGCCCCGAGA CCCAGCAGAT GTTACAGATG TGGTATGACT TGGATGAAGA GAGCCGATGG 
AAATACCAGA AGAGGGCTGC CCCTTGTCCC GACCCTAGCC TTTCTGTGTG GCGCCCAGAC 
ATCTACTTTG CATCTGTGTC TGAAACATTT GAAAAGAAGA TTGATGACTT CAGTCAGGAA 
TGGGCAGCTC AAGCAGAGAG GAGTTACAAG AAATCAGAGC TTTCCCTCGA CAGGCTGAGA 
ACCTTACTGC AGCTGAAGTG GCAGCGCTCA CTGTGTGACC CAGGCGAGGC TGTTGGCCTC 
CTGGCTGCCC AGAGCATCGG AGAGCCCTCC ACACAGATGA CACTGAACAC CTTCCACTTT 
GCAGGCAGAG GCGAGATGAA CGTCACCCTG GGCATTCCAA GATTGAGGGA GATTCTCATG 
GTGGCCAGTG CCAACATCAA AACGCCCATG ATGAGTGTGC CTGTGTTCGA CACCAAGAAA 
GCATTGAAGA AAGTGAAGAG TCTAAAGAAG AGGCTCACCA GAGTGTGCCT GGGGGAGGTA 
TTGCAGAAAG TTGACATCCA GGAGTCCTTC TGTATGGGAG AAAAACGGAA TAAGTTCCAG 
GTGTATGAGC TCCGGTTTCA GTTCCTGCCA CATGCATATT ACCAGCAGGA GAAGTGCCTG 
AGACCCGAGG ACATCTTGCA CTTCATGGAA ACCAGATTCT TTAAGCTACT AATGGAAGCT 
ATCAAAAAGA AGAAAAACAA AGCATCAGCC TTCAGAAATG TGAATTCGAG GAGAGCTACA 
CAGAAGGACC TCAATGACAC TGAGGACTCG GGGAGGAGTC AGAGAGAGGA GGAAAGGGAT 
GAGGAAGAGG AGGGGAACAT TGTGGATGCT GAAGCTGAGG AGGGCGATGC AGACGCTTCT 
GACACCAAAC GCAAGGAGAA GCAAGAAGAA GAGGTTGACT ATGAGAGTGA GGAAGAAGGA 
GAGGAGGAAG AGGAGGAGGA GGTACAAGAA GAAGGGAACA TCAAAGGCGA CGGTGTCCAC 
CAGGGCCATG AGCCAGATGA GGAAGAACAT TTAGGCCTCG AGGAGGAGGA GTCATCCCAG 
AAGCCTCCCC GCCGCCACTC CAGGCCTCAG GGAGCTGAGG CCATAAAACG CCGGATCCAG 
GCTGTACGGG AGTCCTACTC GTTCATCGAG GACTACCAGT ATGACACAGA GGAGAGCCTG 
TGGTGTCAGG TGACTGTGAA GCTCCCACTG ATGAAGATTA ATTTTGACAT GAGCTCCTTG 
GTTGTGTCCT TGGCCCACAA AGCCATTGTC TATACGACCA AGGGCATCAC TCGGTGCCTC 
CTGAACGAAA CGACCAACAG TAAGAATGAG AAGGAGCTTG TACTGAACAC GGAGGGAATC 
AACCTTCCCG AGCTGTTCAA GTACTCCGAG ATACTGGACC TGCGCCGCCT CTACTCCAAT 
GACATCCACG CCATGGCCAA TACTTATGGC ATCGAGGCTG CGTTGCGGGT GATAGAGAAG 
GAGATTAAAG ATGTGTTTGC TGTGTATGGC ATTGCAGTTG ATCCTCGCCA TCTCTCCCTG 
GTGGCTGATT ACATGTGTTT TGAGGGGGTT TATAAGCCAC TCAATCGCTT TGGGATCCAG 
TCAAGCTCCT CCCCTTTGCA GCAGATGACA TTTGAAACCA GCTTCCAGTT TCTGAAACAG 
GCCACCATGA TGGGATCCCA TGATGAACTC AAGTCACCTT CCGCCTGCCT CGTGGTGGGA 
AAGGTTGTCA AAGGCGGGAC AGGCCTGTTT GAGCTCAAGC AGCCCCTAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.
Molecular and cellular biology24(14)6338-49(2004 Jul)
Yamamoto K,Yamamoto M,Hanada K,Nogi Y,Matsuyama T,Muramatsu M

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

A kinetic framework for a mammalian RNA polymerase in vivo.
Science (New York, N.Y.)298(5598)1623-6(2002 Nov)
Dundr M,Hoffmann-Rohrer U,Hu Q,Grummt I,Rothblum LI,Phair RD,Misteli T

Molecular cloning and characterization of the cDNA encoding the largest subunit of mouse RNA polymerase I.
Molecular & general genetics : MGG255(2)180-6(1997 Jun)
Seither P,Coy JF,Pouska A,Grummt I

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Polr1a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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