polr1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	polr1a
Entrez Gene ID	327078
Full Name	polymerase (RNA) I polypeptide A
Synonyms	cb934,fa26a03,sb:cb935,wu:fa26a03
General protein information	Preferred Names polymerase (RNA) I polypeptide A Names DNA-directed RNA polymerase I subunit RPA1 polymerase (RNA) I polypeptide A, 194kDa
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001922804.5	XP_001922839.2	DNA-directed RNA polymerase I subunit RPA1
XM_001922804.6	XP_001922839.2	DNA-directed RNA polymerase I subunit RPA1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
Macaca mulatta (Rhesus monkey)	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Neurospora crassa	NCU01638	XP_956006.2
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3

Gene Ontology

The following polr1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polr1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa19506	XM_001922804.6 Latest version!	Danio rerio polymerase (RNA) I polypeptide A (polr1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa19506	XM_001922804.5	Danio rerio polymerase (RNA) I polypeptide A (polr1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polr1a ( XM_001922804.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922839.2 Danio rerio polymerase (RNA) I polypeptide A (polr1a), mRNA.

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CloneID	ODa19506
Clone ID Related Accession (Same CDS sequence)	XM_001922804.5 , XM_001922804.6
Accession Version	XM_001922804.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922804.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATTTT CAAAAGAAAC ACCATGGAGG CGATTAGAGG GCATGTCGTT TGGCCTGTTC 
TCCGCTGATG AAACAAGAAA ACTGAGTGTG AAAACTATCA CAAATCCTCA GTTGCTGGAT 
AATGTGGGCA ATGCTGCTGC AAACGGGCTG TATGACCTGG CACTCGGTCC GGCTGATAAC 
AAGGAAATCT GTTCTACCTG CATGCAAGAT TTCAGCAGCT GTCCCGGACA CTTTGGCCAC 
ATAGAGCTGC CCCTGCCTGT CTACAACCCT CTGTTTTTTG ATAAATTATA TTTACTCATC 
CGGGGTTCAT GCCTGTCATG TCACATACTG AAGTGTCCAA GAGCAGCTAT TCATTTACTG 
CTGTGCCAGC TGAAGCTCCT GGACGTGGGG GCCATGAAGG AGGTGTTTGA GATTGAAAAC 
GTTCTCAATC AGTTTTTGGA ATCCAACCCC AGCCCACCTG GAGAAGAAAT CCAAGAAACT 
CTGAATGACT TTGTTAAACC CATCCTCGAA ACCAGAAAAG ACAAGCAACA CACTGACCCT 
GTCAAGCACA TCTGTGAGAA AAAGTCCAGT CTCATTACCA GTTTCTGGAG AATACATATG 
GGCCCAAAAA AATGCCAGAA TTGCAAAGCC AATAGAATGA CTGTGCGCAA AGAACACAAC 
AGCAAGCTCA TCGTCACTCA AACTTTGCAC AAAAAAGCTT CTGAACAGGA TGATGATTCC 
CAATTGACCA AACGTATATT CCTGACTCCG AGTGTGGCAC GAGAACACAT CATCAAACTT 
TGGGAGACAG AAGGTTTTTT CCTGAAGCAT TTGTTCTCAG GGTTTGACAT GGACAACTCT 
GATAATGGCT TTAATCCTGA AGTTTTTTTC TTGGAGCTGC TGGTGGTTCC TCCATGCAGG 
TACAGGCCTA TTAATCGTTT GGGAGACCAG ATGTTCACCA ATGGACAGAC TGTGAACATG 
CAAGCTGTGT TAAAGGATGG TTTAATCATT CAGAAATTAC TAACACTAAT TGCTGATGAG 
AAAACCAAGG CTATTGAAGG CACACAGACA GAGCTGGAGC AGTCCAGTGA AGCCCCAAAG 
GTCACTGAAA TTGATCAGAG CTTCCTGACT TCCATCCAAG GAGTGACTCT CACAGACAAG 
CTCTACAGCA CCTGGATCCA TTTGCAGAGT CTTGTCAACA TTGTGTTTGA CAGCGACATG 
GACAAATTGA TCACTGAGAA GTTCCCAGGC GTCCGGCAGC TGTTGGAGAA GAAAGAGGGT 
TTATTTAGGA AGCACATGAT GGGAAAGCGT GTGGATTACG CTGCACGCTC TGTCATCTGT 
CCTGATATGT ACATCGGAAC CAATGAAATT GGAATTCCAA TGGTTTTTGC TACCAAGTTG 
ACATATCCTC AACCTGTGAC ACCCTGGAAC GTAAAGGAAA TGAGGCAGGC AGTGCTGAAT 
GGACCCAACA TCCACCCTGG AGCCACCATG GTTGTTAATG AAGACGGGTC CCGCACTATT 
CTCAGCAGTA CCAATCAGAC CCAAAGAGAA GCTGTCGCCA AACAACTCCT GACACCCAGC 
ACTGGCCAGC ACAGGATACC CATGAAAATT GTAAATCGGC ACATCAAAAA TGGCGATGTG 
CTGCTGTTAA ACCGTCAGCC CACTCTCCAC AGACCATCAA TTCAGGCCCA CTGTGCTCGG 
ATCCTGCCTG GAGAGAAAGT GCTCAGGTTA CATTATGCTA ACTGTAAGGC CTACAACGCT 
GACTTTGATG GAGATGAGAT GAATGCACAC TTCCCTCAGA GTGAGCTGAG CAGAGCTGAG 
GCCTACACAC TAGTGAGCAC AGACCAGCAG TACTTGGTGC CAAAAGATGG GAAACCATTG 
GCAGGCTTGA TTCAAGATCA CATGGTTTCT GGCACCAGCA TGACCATCCG TGGCTGTTTC 
TTCACGAGGG ATCAGTACAC TGCACTAGTC TACAGAGGCC TGACAGATAA GATCGGCAGA 
GTCAAGCTTC TCCCTCCCAC ACTCATTAAG CCTGTGCTGC TGTGGACAGG AAAGCAGGTG 
GTCTCGACCC TTTTGCTGAA TGTCATCCCT GAAAACAGCA TTCCTTTAAA CTTGACTGGA 
AAAGCAAAGA TTCCTGGTAA AGCCTGGGTG AAGGAATCTC CCAGACCTGT TCCAGGATAC 
GTGCCAGAAT CCATGTGTGA ATCTCAGGTG GTCATTCGTC AGGGAGAGCT CTTGATGGGG 
GTTCTGGATA AAGCTCACTA TGGGAGCTCA GCGTATGGTC TGGTCCACTG CTGCTATGAA 
CTCTATGGAG GAGAGACTAG TGGGAAACTG CTTAGCTGTC TGGCACGGCT CTTCACTGCC 
TATCTGCAGC TTTACCGCGG GTTTACTTTG GGTGTGGAGG ACATTCTTGT AAAGGATGGA 
GCCAACAAAC AAAGAAAAAA AATCATCAAA AAGTCAGTCA CATGTGGCTC CAAGGCTCTT 
CAGGCTGCTT TCAACCTGCC TGCTTCTGCT GAAGATACTG AAGCCAGAGA ACGCTGGCAA 
GATGCACATC TGAACATCGA CCAGAGAGAT TTCAACATGG TAGACCTAAA GTTTAAAGAA 
GAGGTCAATC AAGTGAACAA CAACATCAAC AAGGTCTGCA TGCCACTGGG TCTCCACCGC 
TCGTTCCCTG AAAATAACCT ACAAATGATG GTGCAGTCAG GAGCCAAGGG GTCTACTGTT 
AATACTATGC AGATATCCTG TTTGTTGGGT CAAATTGAGC TGGAAGGCCG CCGACCACCA 
CTGATGCCTT CGGGGAAGTC TCTACCCTGC TTTCAGCCCT ATGAGCCACA GCCTCGCTCT 
GGAGGATTTG TGACTGGCAG ATTTCTGACT GGAATCAAAC CTCCTGAATT CTTCTTTCAT 
TGTATGGCTG GTCGTGAAGG TCTTGTGGAC ACTGCAGTGA AAACCAGTCG ATCTGGTTAC 
CTGCAAAGGT GTGTGATTAA GCATCTTGAA GGTTTGGTGG TCCAGTATGA TCTGACTGTG 
CGGGACAGCG ATGGCAGTGT GGTTCAGTTT CTTTATGGAG AGGATGGGTT AGACATTCCC 
AAAACACAAT TCCTACAGCC ACGACAGTTC CCTTTCATCA AAGACAACTA TGAGGTTATC 
AGGAAGTCCA AGTGCTTGGA TGAAGTTTTG GCTAAAATGG ACCCAGTGGC TGCATTAAAT 
CACTTTAAAG CTATAAAGAA ATGGAAAGCC AAGCATGAGC ATGCTTCACC CAGAGATGGT 
GCCTTCTTAC TCTTCTCCCA GAAGAAGATG AGCAAGTTGA AGGCTCAAGT AGAGGGTCAT 
GAGCTAGTCA ATGGGAGAGA GGAGGCCACT TTACAGCTCA TAGAAAGATG GAGAGCTCTG 
GATGAGTCTG GCCGGATGAG GTATTCAAAG AAGACTTCTC ATTGTCCAGA ACCGTGTCTG 
GGTCTTTATC GGCCAGACAT CAGCTTTGGT TCAGTGTCTG AGACATTTGA CTCCATTGTT 
GAGTCTTATC TTAATCAGTT CAACATCAAG CAGGAATTTT CGGCATCTTC TGAAACCACT 
GACGCAGATA GGTTGAGAAA TCTCCTGCAG CTGAAATGGC AGAGAGCTCT GTGTGACCCT 
GGAGAGGCTG TGGGTCTGGT TGCAGCTCAG AGCATCGGTG AACCATCCAC ACAAATGACC 
CTCAACACCT TTCACTTTGC CGGCAGAGGA GAGATGAATG TCACACTGGG TATTCCCAGA 
TTAAGAGAAA TTCTCTTGGT TGCGAGTTCT AACATCAAAA CTCCCATGAT GAGTATTCCA 
GTTGTAATGA ACAAGAAAGC CTTGAAGCAG GTTAAAACAC TGCGCAAGAA GATAACGCGA 
GTCTGCTTAT CAGAGGTGCT ACATAAAGTA AATGTGATAG AATCTTTCCG AATGCCGAAG 
AAGGAGACCC TGAAGTCACG AGTCTTCAAA ATCACGTTCC ACTTCCTGCC ACCTGAGCGA 
TACCAGCAAG ACAAGCTACT CAACCCTGAT GAGATCCTTC ACTACATGGA ACAAACTTTC 
TTCCGCATGC TGTTGGAGGC CATTAAGAAG CTGACTGCAA AATTGGCTGC CATCAGTTTG 
GTGGAAACTC GTAGAGCCAC AGTGAATGAT AATGATCGGG ATACCAGTGA TGGCGGCGCA 
GACAGAGAGG ACAATGGGGG AGAGGATGCA GAAAACGAGA GGATTGTGGA TGATGAGGCC 
AATGAAGGTG ATGCTGATGC CACAGATTCC AAGAGAAAAG AAAAGCAAGA AGAGGAGGTA 
GACTATGAGA GCGAGGAGGG AGAGGAAGGC AGTGATCAGG AGCAGGAGGA AGTGGCTGAG 
GAACAGGAGG CGTCTCAGGA GGTCTCTGAG GAAGGGTCTA CTGAGAGCCA ACAGAGAGTT 
AACTCTGAAC AGCCGAAGGG AAGCTCACAG GGCTCAGTGA GGATCAACTC TGTGCTGCAG 
TTAAGCGCTG CTATTGAGGA CTACAAATAT GACACCAAAA AAGGACTGTG GTGTGAGTTA 
ACCCTGGTCA TGCCGATCAC TAAAGTGCAC TTTGACCTCA CCTCAGTGGT GGTGAAACAG 
GCTCAGAACG CGGTCATCAT GGAGACCAAG GGCATCACGC GCTGTTTGTT AAATGAAGTG 
ACCAGAAAAG ACGGAAGCAA AGAGATTATG CTCAACACAG AAGGCATCAA CATTCATGAG 
ATGTTCAAAT ATGCTGATAT CCTGGACTTG AAGAGATTAT ACTCCAATGA GGTTCATGCC 
ATGGCCAGCA CATATGGTAT TGAGGTGGCC CTTAGAGTGA TCGAGAAGGA GATTAAAGAT 
GTGTTTGCTG TATACGGTAT CGAAGTGGAT CCTCGTCATC TGTCTTTAGT TGCTGATTAC 
ATGTGTTTTG AGGGAGTGTA CAAGCCTCTC AATCGTTTCG GCATTCAGTC CAACAGCTCC 
CCACTGCAAC AGATGACCTT TGAGACCAGC TTCAAGTTCC TGAAGCAAGC CACGATGTTA 
GGTTCTTATG ATAAACTCAG TTCGCCCTCA GCTTGTTTGG TTGTAGGAAA AGTTGTGAAG 
GGAGGAACTG GAATATTTGA TCTGAAACAG CCATTACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922839.2
CDS	113..5194
Translation	MQFSKETPWRRLEGMSFGLFSADETRKLSVKTITNPQLLDNVGNAAANGLYDLALGPADNKEICSTCMQDFSSCPGHFGHIELPLPVYNPLFFDKLYLLIRGSCLSCHILKCPRAAIHLLLCQLKLLDVGAMKEVFEIENVLNQFLESNPSPPGEEIQETLNDFVKPILETRKDKQHTDPVKHICEKKSSLITSFWRIHMGPKKCQNCKANRMTVRKEHNSKLIVTQTLHKKASEQDDDSQLTKRIFLTPSVAREHIIKLWETEGFFLKHLFSGFDMDNSDNGFNPEVFFLELLVVPPCRYRPINRLGDQMFTNGQTVNMQAVLKDGLIIQKLLTLIADEKTKAIEGTQTELEQSSEAPKVTEIDQSFLTSIQGVTLTDKLYSTWIHLQSLVNIVFDSDMDKLITEKFPGVRQLLEKKEGLFRKHMMGKRVDYAARSVICPDMYIGTNEIGIPMVFATKLTYPQPVTPWNVKEMRQAVLNGPNIHPGATMVVNEDGSRTILSSTNQTQREAVAKQLLTPSTGQHRIPMKIVNRHIKNGDVLLLNRQPTLHRPSIQAHCARILPGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELSRAEAYTLVSTDQQYLVPKDGKPLAGLIQDHMVSGTSMTIRGCFFTRDQYTALVYRGLTDKIGRVKLLPPTLIKPVLLWTGKQVVSTLLLNVIPENSIPLNLTGKAKIPGKAWVKESPRPVPGYVPESMCESQVVIRQGELLMGVLDKAHYGSSAYGLVHCCYELYGGETSGKLLSCLARLFTAYLQLYRGFTLGVEDILVKDGANKQRKKIIKKSVTCGSKALQAAFNLPASAEDTEARERWQDAHLNIDQRDFNMVDLKFKEEVNQVNNNINKVCMPLGLHRSFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGKSLPCFQPYEPQPRSGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCVIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPRQFPFIKDNYEVIRKSKCLDEVLAKMDPVAALNHFKAIKKWKAKHEHASPRDGAFLLFSQKKMSKLKAQVEGHELVNGREEATLQLIERWRALDESGRMRYSKKTSHCPEPCLGLYRPDISFGSVSETFDSIVESYLNQFNIKQEFSASSETTDADRLRNLLQLKWQRALCDPGEAVGLVAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILLVASSNIKTPMMSIPVVMNKKALKQVKTLRKKITRVCLSEVLHKVNVIESFRMPKKETLKSRVFKITFHFLPPERYQQDKLLNPDEILHYMEQTFFRMLLEAIKKLTAKLAAISLVETRRATVNDNDRDTSDGGADREDNGGEDAENERIVDDEANEGDADATDSKRKEKQEEEVDYESEEGEEGSDQEQEEVAEEQEASQEVSEEGSTESQQRVNSEQPKGSSQGSVRINSVLQLSAAIEDYKYDTKKGLWCELTLVMPITKVHFDLTSVVVKQAQNAVIMETKGITRCLLNEVTRKDGSKEIMLNTEGINIHEMFKYADILDLKRLYSNEVHAMASTYGIEVALRVIEKEIKDVFAVYGIEVDPRHLSLVADYMCFEGVYKPLNRFGIQSNSSPLQQMTFETSFKFLKQATMLGSYDKLSSPSACLVVGKVVKGGTGIFDLKQPLQ

Target ORF information:

RefSeq Version	XM_001922804.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (RNA) I polypeptide A (polr1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922804.5

ORF Insert Sequence:

ATGCAATTTT CAAAAGAAAC ACCATGGAGG CGATTAGAGG GCATGTCGTT TGGCCTGTTC 
TCCGCTGATG AAACAAGAAA ACTGAGTGTG AAAACTATCA CAAATCCTCA GTTGCTGGAT 
AATGTGGGCA ATGCTGCTGC AAACGGGCTG TATGACCTGG CACTCGGTCC GGCTGATAAC 
AAGGAAATCT GTTCTACCTG CATGCAAGAT TTCAGCAGCT GTCCCGGACA CTTTGGCCAC 
ATAGAGCTGC CCCTGCCTGT CTACAACCCT CTGTTTTTTG ATAAATTATA TTTACTCATC 
CGGGGTTCAT GCCTGTCATG TCACATACTG AAGTGTCCAA GAGCAGCTAT TCATTTACTG 
CTGTGCCAGC TGAAGCTCCT GGACGTGGGG GCCATGAAGG AGGTGTTTGA GATTGAAAAC 
GTTCTCAATC AGTTTTTGGA ATCCAACCCC AGCCCACCTG GAGAAGAAAT CCAAGAAACT 
CTGAATGACT TTGTTAAACC CATCCTCGAA ACCAGAAAAG ACAAGCAACA CACTGACCCT 
GTCAAGCACA TCTGTGAGAA AAAGTCCAGT CTCATTACCA GTTTCTGGAG AATACATATG 
GGCCCAAAAA AATGCCAGAA TTGCAAAGCC AATAGAATGA CTGTGCGCAA AGAACACAAC 
AGCAAGCTCA TCGTCACTCA AACTTTGCAC AAAAAAGCTT CTGAACAGGA TGATGATTCC 
CAATTGACCA AACGTATATT CCTGACTCCG AGTGTGGCAC GAGAACACAT CATCAAACTT 
TGGGAGACAG AAGGTTTTTT CCTGAAGCAT TTGTTCTCAG GGTTTGACAT GGACAACTCT 
GATAATGGCT TTAATCCTGA AGTTTTTTTC TTGGAGCTGC TGGTGGTTCC TCCATGCAGG 
TACAGGCCTA TTAATCGTTT GGGAGACCAG ATGTTCACCA ATGGACAGAC TGTGAACATG 
CAAGCTGTGT TAAAGGATGG TTTAATCATT CAGAAATTAC TAACACTAAT TGCTGATGAG 
AAAACCAAGG CTATTGAAGG CACACAGACA GAGCTGGAGC AGTCCAGTGA AGCCCCAAAG 
GTCACTGAAA TTGATCAGAG CTTCCTGACT TCCATCCAAG GAGTGACTCT CACAGACAAG 
CTCTACAGCA CCTGGATCCA TTTGCAGAGT CTTGTCAACA TTGTGTTTGA CAGCGACATG 
GACAAATTGA TCACTGAGAA GTTCCCAGGC GTCCGGCAGC TGTTGGAGAA GAAAGAGGGT 
TTATTTAGGA AGCACATGAT GGGAAAGCGT GTGGATTACG CTGCACGCTC TGTCATCTGT 
CCTGATATGT ACATCGGAAC CAATGAAATT GGAATTCCAA TGGTTTTTGC TACCAAGTTG 
ACATATCCTC AACCTGTGAC ACCCTGGAAC GTAAAGGAAA TGAGGCAGGC AGTGCTGAAT 
GGACCCAACA TCCACCCTGG AGCCACCATG GTTGTTAATG AAGACGGGTC CCGCACTATT 
CTCAGCAGTA CCAATCAGAC CCAAAGAGAA GCTGTCGCCA AACAACTCCT GACACCCAGC 
ACTGGCCAGC ACAGGATACC CATGAAAATT GTAAATCGGC ACATCAAAAA TGGCGATGTG 
CTGCTGTTAA ACCGTCAGCC CACTCTCCAC AGACCATCAA TTCAGGCCCA CTGTGCTCGG 
ATCCTGCCTG GAGAGAAAGT GCTCAGGTTA CATTATGCTA ACTGTAAGGC CTACAACGCT 
GACTTTGATG GAGATGAGAT GAATGCACAC TTCCCTCAGA GTGAGCTGAG CAGAGCTGAG 
GCCTACACAC TAGTGAGCAC AGACCAGCAG TACTTGGTGC CAAAAGATGG GAAACCATTG 
GCAGGCTTGA TTCAAGATCA CATGGTTTCT GGCACCAGCA TGACCATCCG TGGCTGTTTC 
TTCACGAGGG ATCAGTACAC TGCACTAGTC TACAGAGGCC TGACAGATAA GATCGGCAGA 
GTCAAGCTTC TCCCTCCCAC ACTCATTAAG CCTGTGCTGC TGTGGACAGG AAAGCAGGTG 
GTCTCGACCC TTTTGCTGAA TGTCATCCCT GAAAACAGCA TTCCTTTAAA CTTGACTGGA 
AAAGCAAAGA TTCCTGGTAA AGCCTGGGTG AAGGAATCTC CCAGACCTGT TCCAGGATAC 
GTGCCAGAAT CCATGTGTGA ATCTCAGGTG GTCATTCGTC AGGGAGAGCT CTTGATGGGG 
GTTCTGGATA AAGCTCACTA TGGGAGCTCA GCGTATGGTC TGGTCCACTG CTGCTATGAA 
CTCTATGGAG GAGAGACTAG TGGGAAACTG CTTAGCTGTC TGGCACGGCT CTTCACTGCC 
TATCTGCAGC TTTACCGCGG GTTTACTTTG GGTGTGGAGG ACATTCTTGT AAAGGATGGA 
GCCAACAAAC AAAGAAAAAA AATCATCAAA AAGTCAGTCA CATGTGGCTC CAAGGCTCTT 
CAGGCTGCTT TCAACCTGCC TGCTTCTGCT GAAGATACTG AAGCCAGAGA ACGCTGGCAA 
GATGCACATC TGAACATCGA CCAGAGAGAT TTCAACATGG TAGACCTAAA GTTTAAAGAA 
GAGGTCAATC AAGTGAACAA CAACATCAAC AAGGTCTGCA TGCCACTGGG TCTCCACCGC 
TCGTTCCCTG AAAATAACCT ACAAATGATG GTGCAGTCAG GAGCCAAGGG GTCTACTGTT 
AATACTATGC AGATATCCTG TTTGTTGGGT CAAATTGAGC TGGAAGGCCG CCGACCACCA 
CTGATGCCTT CGGGGAAGTC TCTACCCTGC TTTCAGCCCT ATGAGCCACA GCCTCGCTCT 
GGAGGATTTG TGACTGGCAG ATTTCTGACT GGAATCAAAC CTCCTGAATT CTTCTTTCAT 
TGTATGGCTG GTCGTGAAGG TCTTGTGGAC ACTGCAGTGA AAACCAGTCG ATCTGGTTAC 
CTGCAAAGGT GTGTGATTAA GCATCTTGAA GGTTTGGTGG TCCAGTATGA TCTGACTGTG 
CGGGACAGCG ATGGCAGTGT GGTTCAGTTT CTTTATGGAG AGGATGGGTT AGACATTCCC 
AAAACACAAT TCCTACAGCC ACGACAGTTC CCTTTCATCA AAGACAACTA TGAGGTTATC 
AGGAAGTCCA AGTGCTTGGA TGAAGTTTTG GCTAAAATGG ACCCAGTGGC TGCATTAAAT 
CACTTTAAAG CTATAAAGAA ATGGAAAGCC AAGCATGAGC ATGCTTCACC CAGAGATGGT 
GCCTTCTTAC TCTTCTCCCA GAAGAAGATG AGCAAGTTGA AGGCTCAAGT AGAGGGTCAT 
GAGCTAGTCA ATGGGAGAGA GGAGGCCACT TTACAGCTCA TAGAAAGATG GAGAGCTCTG 
GATGAGTCTG GCCGGATGAG GTATTCAAAG AAGACTTCTC ATTGTCCAGA ACCGTGTCTG 
GGTCTTTATC GGCCAGACAT CAGCTTTGGT TCAGTGTCTG AGACATTTGA CTCCATTGTT 
GAGTCTTATC TTAATCAGTT CAACATCAAG CAGGAATTTT CGGCATCTTC TGAAACCACT 
GACGCAGATA GGTTGAGAAA TCTCCTGCAG CTGAAATGGC AGAGAGCTCT GTGTGACCCT 
GGAGAGGCTG TGGGTCTGGT TGCAGCTCAG AGCATCGGTG AACCATCCAC ACAAATGACC 
CTCAACACCT TTCACTTTGC CGGCAGAGGA GAGATGAATG TCACACTGGG TATTCCCAGA 
TTAAGAGAAA TTCTCTTGGT TGCGAGTTCT AACATCAAAA CTCCCATGAT GAGTATTCCA 
GTTGTAATGA ACAAGAAAGC CTTGAAGCAG GTTAAAACAC TGCGCAAGAA GATAACGCGA 
GTCTGCTTAT CAGAGGTGCT ACATAAAGTA AATGTGATAG AATCTTTCCG AATGCCGAAG 
AAGGAGACCC TGAAGTCACG AGTCTTCAAA ATCACGTTCC ACTTCCTGCC ACCTGAGCGA 
TACCAGCAAG ACAAGCTACT CAACCCTGAT GAGATCCTTC ACTACATGGA ACAAACTTTC 
TTCCGCATGC TGTTGGAGGC CATTAAGAAG CTGACTGCAA AATTGGCTGC CATCAGTTTG 
GTGGAAACTC GTAGAGCCAC AGTGAATGAT AATGATCGGG ATACCAGTGA TGGCGGCGCA 
GACAGAGAGG ACAATGGGGG AGAGGATGCA GAAAACGAGA GGATTGTGGA TGATGAGGCC 
AATGAAGGTG ATGCTGATGC CACAGATTCC AAGAGAAAAG AAAAGCAAGA AGAGGAGGTA 
GACTATGAGA GCGAGGAGGG AGAGGAAGGC AGTGATCAGG AGCAGGAGGA AGTGGCTGAG 
GAACAGGAGG CGTCTCAGGA GGTCTCTGAG GAAGGGTCTA CTGAGAGCCA ACAGAGAGTT 
AACTCTGAAC AGCCGAAGGG AAGCTCACAG GGCTCAGTGA GGATCAACTC TGTGCTGCAG 
TTAAGCGCTG CTATTGAGGA CTACAAATAT GACACCAAAA AAGGACTGTG GTGTGAGTTA 
ACCCTGGTCA TGCCGATCAC TAAAGTGCAC TTTGACCTCA CCTCAGTGGT GGTGAAACAG 
GCTCAGAACG CGGTCATCAT GGAGACCAAG GGCATCACGC GCTGTTTGTT AAATGAAGTG 
ACCAGAAAAG ACGGAAGCAA AGAGATTATG CTCAACACAG AAGGCATCAA CATTCATGAG 
ATGTTCAAAT ATGCTGATAT CCTGGACTTG AAGAGATTAT ACTCCAATGA GGTTCATGCC 
ATGGCCAGCA CATATGGTAT TGAGGTGGCC CTTAGAGTGA TCGAGAAGGA GATTAAAGAT 
GTGTTTGCTG TATACGGTAT CGAAGTGGAT CCTCGTCATC TGTCTTTAGT TGCTGATTAC 
ATGTGTTTTG AGGGAGTGTA CAAGCCTCTC AATCGTTTCG GCATTCAGTC CAACAGCTCC 
CCACTGCAAC AGATGACCTT TGAGACCAGC TTCAAGTTCC TGAAGCAAGC CACGATGTTA 
GGTTCTTATG ATAAACTCAG TTCGCCCTCA GCTTGTTTGG TTGTAGGAAA AGTTGTGAAG 
GGAGGAACTG GAATATTTGA TCTGAAACAG CCATTACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

polr1a ( XM_001922804.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922839.2 Danio rerio polymerase (RNA) I polypeptide A (polr1a), mRNA.

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CloneID	ODa19506
Clone ID Related Accession (Same CDS sequence)	XM_001922804.5 , XM_001922804.6
Accession Version	XM_001922804.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922804.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATTTT CAAAAGAAAC ACCATGGAGG CGATTAGAGG GCATGTCGTT TGGCCTGTTC 
TCCGCTGATG AAACAAGAAA ACTGAGTGTG AAAACTATCA CAAATCCTCA GTTGCTGGAT 
AATGTGGGCA ATGCTGCTGC AAACGGGCTG TATGACCTGG CACTCGGTCC GGCTGATAAC 
AAGGAAATCT GTTCTACCTG CATGCAAGAT TTCAGCAGCT GTCCCGGACA CTTTGGCCAC 
ATAGAGCTGC CCCTGCCTGT CTACAACCCT CTGTTTTTTG ATAAATTATA TTTACTCATC 
CGGGGTTCAT GCCTGTCATG TCACATACTG AAGTGTCCAA GAGCAGCTAT TCATTTACTG 
CTGTGCCAGC TGAAGCTCCT GGACGTGGGG GCCATGAAGG AGGTGTTTGA GATTGAAAAC 
GTTCTCAATC AGTTTTTGGA ATCCAACCCC AGCCCACCTG GAGAAGAAAT CCAAGAAACT 
CTGAATGACT TTGTTAAACC CATCCTCGAA ACCAGAAAAG ACAAGCAACA CACTGACCCT 
GTCAAGCACA TCTGTGAGAA AAAGTCCAGT CTCATTACCA GTTTCTGGAG AATACATATG 
GGCCCAAAAA AATGCCAGAA TTGCAAAGCC AATAGAATGA CTGTGCGCAA AGAACACAAC 
AGCAAGCTCA TCGTCACTCA AACTTTGCAC AAAAAAGCTT CTGAACAGGA TGATGATTCC 
CAATTGACCA AACGTATATT CCTGACTCCG AGTGTGGCAC GAGAACACAT CATCAAACTT 
TGGGAGACAG AAGGTTTTTT CCTGAAGCAT TTGTTCTCAG GGTTTGACAT GGACAACTCT 
GATAATGGCT TTAATCCTGA AGTTTTTTTC TTGGAGCTGC TGGTGGTTCC TCCATGCAGG 
TACAGGCCTA TTAATCGTTT GGGAGACCAG ATGTTCACCA ATGGACAGAC TGTGAACATG 
CAAGCTGTGT TAAAGGATGG TTTAATCATT CAGAAATTAC TAACACTAAT TGCTGATGAG 
AAAACCAAGG CTATTGAAGG CACACAGACA GAGCTGGAGC AGTCCAGTGA AGCCCCAAAG 
GTCACTGAAA TTGATCAGAG CTTCCTGACT TCCATCCAAG GAGTGACTCT CACAGACAAG 
CTCTACAGCA CCTGGATCCA TTTGCAGAGT CTTGTCAACA TTGTGTTTGA CAGCGACATG 
GACAAATTGA TCACTGAGAA GTTCCCAGGC GTCCGGCAGC TGTTGGAGAA GAAAGAGGGT 
TTATTTAGGA AGCACATGAT GGGAAAGCGT GTGGATTACG CTGCACGCTC TGTCATCTGT 
CCTGATATGT ACATCGGAAC CAATGAAATT GGAATTCCAA TGGTTTTTGC TACCAAGTTG 
ACATATCCTC AACCTGTGAC ACCCTGGAAC GTAAAGGAAA TGAGGCAGGC AGTGCTGAAT 
GGACCCAACA TCCACCCTGG AGCCACCATG GTTGTTAATG AAGACGGGTC CCGCACTATT 
CTCAGCAGTA CCAATCAGAC CCAAAGAGAA GCTGTCGCCA AACAACTCCT GACACCCAGC 
ACTGGCCAGC ACAGGATACC CATGAAAATT GTAAATCGGC ACATCAAAAA TGGCGATGTG 
CTGCTGTTAA ACCGTCAGCC CACTCTCCAC AGACCATCAA TTCAGGCCCA CTGTGCTCGG 
ATCCTGCCTG GAGAGAAAGT GCTCAGGTTA CATTATGCTA ACTGTAAGGC CTACAACGCT 
GACTTTGATG GAGATGAGAT GAATGCACAC TTCCCTCAGA GTGAGCTGAG CAGAGCTGAG 
GCCTACACAC TAGTGAGCAC AGACCAGCAG TACTTGGTGC CAAAAGATGG GAAACCATTG 
GCAGGCTTGA TTCAAGATCA CATGGTTTCT GGCACCAGCA TGACCATCCG TGGCTGTTTC 
TTCACGAGGG ATCAGTACAC TGCACTAGTC TACAGAGGCC TGACAGATAA GATCGGCAGA 
GTCAAGCTTC TCCCTCCCAC ACTCATTAAG CCTGTGCTGC TGTGGACAGG AAAGCAGGTG 
GTCTCGACCC TTTTGCTGAA TGTCATCCCT GAAAACAGCA TTCCTTTAAA CTTGACTGGA 
AAAGCAAAGA TTCCTGGTAA AGCCTGGGTG AAGGAATCTC CCAGACCTGT TCCAGGATAC 
GTGCCAGAAT CCATGTGTGA ATCTCAGGTG GTCATTCGTC AGGGAGAGCT CTTGATGGGG 
GTTCTGGATA AAGCTCACTA TGGGAGCTCA GCGTATGGTC TGGTCCACTG CTGCTATGAA 
CTCTATGGAG GAGAGACTAG TGGGAAACTG CTTAGCTGTC TGGCACGGCT CTTCACTGCC 
TATCTGCAGC TTTACCGCGG GTTTACTTTG GGTGTGGAGG ACATTCTTGT AAAGGATGGA 
GCCAACAAAC AAAGAAAAAA AATCATCAAA AAGTCAGTCA CATGTGGCTC CAAGGCTCTT 
CAGGCTGCTT TCAACCTGCC TGCTTCTGCT GAAGATACTG AAGCCAGAGA ACGCTGGCAA 
GATGCACATC TGAACATCGA CCAGAGAGAT TTCAACATGG TAGACCTAAA GTTTAAAGAA 
GAGGTCAATC AAGTGAACAA CAACATCAAC AAGGTCTGCA TGCCACTGGG TCTCCACCGC 
TCGTTCCCTG AAAATAACCT ACAAATGATG GTGCAGTCAG GAGCCAAGGG GTCTACTGTT 
AATACTATGC AGATATCCTG TTTGTTGGGT CAAATTGAGC TGGAAGGCCG CCGACCACCA 
CTGATGCCTT CGGGGAAGTC TCTACCCTGC TTTCAGCCCT ATGAGCCACA GCCTCGCTCT 
GGAGGATTTG TGACTGGCAG ATTTCTGACT GGAATCAAAC CTCCTGAATT CTTCTTTCAT 
TGTATGGCTG GTCGTGAAGG TCTTGTGGAC ACTGCAGTGA AAACCAGTCG ATCTGGTTAC 
CTGCAAAGGT GTGTGATTAA GCATCTTGAA GGTTTGGTGG TCCAGTATGA TCTGACTGTG 
CGGGACAGCG ATGGCAGTGT GGTTCAGTTT CTTTATGGAG AGGATGGGTT AGACATTCCC 
AAAACACAAT TCCTACAGCC ACGACAGTTC CCTTTCATCA AAGACAACTA TGAGGTTATC 
AGGAAGTCCA AGTGCTTGGA TGAAGTTTTG GCTAAAATGG ACCCAGTGGC TGCATTAAAT 
CACTTTAAAG CTATAAAGAA ATGGAAAGCC AAGCATGAGC ATGCTTCACC CAGAGATGGT 
GCCTTCTTAC TCTTCTCCCA GAAGAAGATG AGCAAGTTGA AGGCTCAAGT AGAGGGTCAT 
GAGCTAGTCA ATGGGAGAGA GGAGGCCACT TTACAGCTCA TAGAAAGATG GAGAGCTCTG 
GATGAGTCTG GCCGGATGAG GTATTCAAAG AAGACTTCTC ATTGTCCAGA ACCGTGTCTG 
GGTCTTTATC GGCCAGACAT CAGCTTTGGT TCAGTGTCTG AGACATTTGA CTCCATTGTT 
GAGTCTTATC TTAATCAGTT CAACATCAAG CAGGAATTTT CGGCATCTTC TGAAACCACT 
GACGCAGATA GGTTGAGAAA TCTCCTGCAG CTGAAATGGC AGAGAGCTCT GTGTGACCCT 
GGAGAGGCTG TGGGTCTGGT TGCAGCTCAG AGCATCGGTG AACCATCCAC ACAAATGACC 
CTCAACACCT TTCACTTTGC CGGCAGAGGA GAGATGAATG TCACACTGGG TATTCCCAGA 
TTAAGAGAAA TTCTCTTGGT TGCGAGTTCT AACATCAAAA CTCCCATGAT GAGTATTCCA 
GTTGTAATGA ACAAGAAAGC CTTGAAGCAG GTTAAAACAC TGCGCAAGAA GATAACGCGA 
GTCTGCTTAT CAGAGGTGCT ACATAAAGTA AATGTGATAG AATCTTTCCG AATGCCGAAG 
AAGGAGACCC TGAAGTCACG AGTCTTCAAA ATCACGTTCC ACTTCCTGCC ACCTGAGCGA 
TACCAGCAAG ACAAGCTACT CAACCCTGAT GAGATCCTTC ACTACATGGA ACAAACTTTC 
TTCCGCATGC TGTTGGAGGC CATTAAGAAG CTGACTGCAA AATTGGCTGC CATCAGTTTG 
GTGGAAACTC GTAGAGCCAC AGTGAATGAT AATGATCGGG ATACCAGTGA TGGCGGCGCA 
GACAGAGAGG ACAATGGGGG AGAGGATGCA GAAAACGAGA GGATTGTGGA TGATGAGGCC 
AATGAAGGTG ATGCTGATGC CACAGATTCC AAGAGAAAAG AAAAGCAAGA AGAGGAGGTA 
GACTATGAGA GCGAGGAGGG AGAGGAAGGC AGTGATCAGG AGCAGGAGGA AGTGGCTGAG 
GAACAGGAGG CGTCTCAGGA GGTCTCTGAG GAAGGGTCTA CTGAGAGCCA ACAGAGAGTT 
AACTCTGAAC AGCCGAAGGG AAGCTCACAG GGCTCAGTGA GGATCAACTC TGTGCTGCAG 
TTAAGCGCTG CTATTGAGGA CTACAAATAT GACACCAAAA AAGGACTGTG GTGTGAGTTA 
ACCCTGGTCA TGCCGATCAC TAAAGTGCAC TTTGACCTCA CCTCAGTGGT GGTGAAACAG 
GCTCAGAACG CGGTCATCAT GGAGACCAAG GGCATCACGC GCTGTTTGTT AAATGAAGTG 
ACCAGAAAAG ACGGAAGCAA AGAGATTATG CTCAACACAG AAGGCATCAA CATTCATGAG 
ATGTTCAAAT ATGCTGATAT CCTGGACTTG AAGAGATTAT ACTCCAATGA GGTTCATGCC 
ATGGCCAGCA CATATGGTAT TGAGGTGGCC CTTAGAGTGA TCGAGAAGGA GATTAAAGAT 
GTGTTTGCTG TATACGGTAT CGAAGTGGAT CCTCGTCATC TGTCTTTAGT TGCTGATTAC 
ATGTGTTTTG AGGGAGTGTA CAAGCCTCTC AATCGTTTCG GCATTCAGTC CAACAGCTCC 
CCACTGCAAC AGATGACCTT TGAGACCAGC TTCAAGTTCC TGAAGCAAGC CACGATGTTA 
GGTTCTTATG ATAAACTCAG TTCGCCCTCA GCTTGTTTGG TTGTAGGAAA AGTTGTGAAG 
GGAGGAACTG GAATATTTGA TCTGAAACAG CCATTACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922839.2
CDS	110..5191
Translation	MQFSKETPWRRLEGMSFGLFSADETRKLSVKTITNPQLLDNVGNAAANGLYDLALGPADNKEICSTCMQDFSSCPGHFGHIELPLPVYNPLFFDKLYLLIRGSCLSCHILKCPRAAIHLLLCQLKLLDVGAMKEVFEIENVLNQFLESNPSPPGEEIQETLNDFVKPILETRKDKQHTDPVKHICEKKSSLITSFWRIHMGPKKCQNCKANRMTVRKEHNSKLIVTQTLHKKASEQDDDSQLTKRIFLTPSVAREHIIKLWETEGFFLKHLFSGFDMDNSDNGFNPEVFFLELLVVPPCRYRPINRLGDQMFTNGQTVNMQAVLKDGLIIQKLLTLIADEKTKAIEGTQTELEQSSEAPKVTEIDQSFLTSIQGVTLTDKLYSTWIHLQSLVNIVFDSDMDKLITEKFPGVRQLLEKKEGLFRKHMMGKRVDYAARSVICPDMYIGTNEIGIPMVFATKLTYPQPVTPWNVKEMRQAVLNGPNIHPGATMVVNEDGSRTILSSTNQTQREAVAKQLLTPSTGQHRIPMKIVNRHIKNGDVLLLNRQPTLHRPSIQAHCARILPGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELSRAEAYTLVSTDQQYLVPKDGKPLAGLIQDHMVSGTSMTIRGCFFTRDQYTALVYRGLTDKIGRVKLLPPTLIKPVLLWTGKQVVSTLLLNVIPENSIPLNLTGKAKIPGKAWVKESPRPVPGYVPESMCESQVVIRQGELLMGVLDKAHYGSSAYGLVHCCYELYGGETSGKLLSCLARLFTAYLQLYRGFTLGVEDILVKDGANKQRKKIIKKSVTCGSKALQAAFNLPASAEDTEARERWQDAHLNIDQRDFNMVDLKFKEEVNQVNNNINKVCMPLGLHRSFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGKSLPCFQPYEPQPRSGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCVIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPRQFPFIKDNYEVIRKSKCLDEVLAKMDPVAALNHFKAIKKWKAKHEHASPRDGAFLLFSQKKMSKLKAQVEGHELVNGREEATLQLIERWRALDESGRMRYSKKTSHCPEPCLGLYRPDISFGSVSETFDSIVESYLNQFNIKQEFSASSETTDADRLRNLLQLKWQRALCDPGEAVGLVAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILLVASSNIKTPMMSIPVVMNKKALKQVKTLRKKITRVCLSEVLHKVNVIESFRMPKKETLKSRVFKITFHFLPPERYQQDKLLNPDEILHYMEQTFFRMLLEAIKKLTAKLAAISLVETRRATVNDNDRDTSDGGADREDNGGEDAENERIVDDEANEGDADATDSKRKEKQEEEVDYESEEGEEGSDQEQEEVAEEQEASQEVSEEGSTESQQRVNSEQPKGSSQGSVRINSVLQLSAAIEDYKYDTKKGLWCELTLVMPITKVHFDLTSVVVKQAQNAVIMETKGITRCLLNEVTRKDGSKEIMLNTEGINIHEMFKYADILDLKRLYSNEVHAMASTYGIEVALRVIEKEIKDVFAVYGIEVDPRHLSLVADYMCFEGVYKPLNRFGIQSNSSPLQQMTFETSFKFLKQATMLGSYDKLSSPSACLVVGKVVKGGTGIFDLKQPLQ

Target ORF information:

RefSeq Version	XM_001922804.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (RNA) I polypeptide A (polr1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922804.6

ORF Insert Sequence:

ATGCAATTTT CAAAAGAAAC ACCATGGAGG CGATTAGAGG GCATGTCGTT TGGCCTGTTC 
TCCGCTGATG AAACAAGAAA ACTGAGTGTG AAAACTATCA CAAATCCTCA GTTGCTGGAT 
AATGTGGGCA ATGCTGCTGC AAACGGGCTG TATGACCTGG CACTCGGTCC GGCTGATAAC 
AAGGAAATCT GTTCTACCTG CATGCAAGAT TTCAGCAGCT GTCCCGGACA CTTTGGCCAC 
ATAGAGCTGC CCCTGCCTGT CTACAACCCT CTGTTTTTTG ATAAATTATA TTTACTCATC 
CGGGGTTCAT GCCTGTCATG TCACATACTG AAGTGTCCAA GAGCAGCTAT TCATTTACTG 
CTGTGCCAGC TGAAGCTCCT GGACGTGGGG GCCATGAAGG AGGTGTTTGA GATTGAAAAC 
GTTCTCAATC AGTTTTTGGA ATCCAACCCC AGCCCACCTG GAGAAGAAAT CCAAGAAACT 
CTGAATGACT TTGTTAAACC CATCCTCGAA ACCAGAAAAG ACAAGCAACA CACTGACCCT 
GTCAAGCACA TCTGTGAGAA AAAGTCCAGT CTCATTACCA GTTTCTGGAG AATACATATG 
GGCCCAAAAA AATGCCAGAA TTGCAAAGCC AATAGAATGA CTGTGCGCAA AGAACACAAC 
AGCAAGCTCA TCGTCACTCA AACTTTGCAC AAAAAAGCTT CTGAACAGGA TGATGATTCC 
CAATTGACCA AACGTATATT CCTGACTCCG AGTGTGGCAC GAGAACACAT CATCAAACTT 
TGGGAGACAG AAGGTTTTTT CCTGAAGCAT TTGTTCTCAG GGTTTGACAT GGACAACTCT 
GATAATGGCT TTAATCCTGA AGTTTTTTTC TTGGAGCTGC TGGTGGTTCC TCCATGCAGG 
TACAGGCCTA TTAATCGTTT GGGAGACCAG ATGTTCACCA ATGGACAGAC TGTGAACATG 
CAAGCTGTGT TAAAGGATGG TTTAATCATT CAGAAATTAC TAACACTAAT TGCTGATGAG 
AAAACCAAGG CTATTGAAGG CACACAGACA GAGCTGGAGC AGTCCAGTGA AGCCCCAAAG 
GTCACTGAAA TTGATCAGAG CTTCCTGACT TCCATCCAAG GAGTGACTCT CACAGACAAG 
CTCTACAGCA CCTGGATCCA TTTGCAGAGT CTTGTCAACA TTGTGTTTGA CAGCGACATG 
GACAAATTGA TCACTGAGAA GTTCCCAGGC GTCCGGCAGC TGTTGGAGAA GAAAGAGGGT 
TTATTTAGGA AGCACATGAT GGGAAAGCGT GTGGATTACG CTGCACGCTC TGTCATCTGT 
CCTGATATGT ACATCGGAAC CAATGAAATT GGAATTCCAA TGGTTTTTGC TACCAAGTTG 
ACATATCCTC AACCTGTGAC ACCCTGGAAC GTAAAGGAAA TGAGGCAGGC AGTGCTGAAT 
GGACCCAACA TCCACCCTGG AGCCACCATG GTTGTTAATG AAGACGGGTC CCGCACTATT 
CTCAGCAGTA CCAATCAGAC CCAAAGAGAA GCTGTCGCCA AACAACTCCT GACACCCAGC 
ACTGGCCAGC ACAGGATACC CATGAAAATT GTAAATCGGC ACATCAAAAA TGGCGATGTG 
CTGCTGTTAA ACCGTCAGCC CACTCTCCAC AGACCATCAA TTCAGGCCCA CTGTGCTCGG 
ATCCTGCCTG GAGAGAAAGT GCTCAGGTTA CATTATGCTA ACTGTAAGGC CTACAACGCT 
GACTTTGATG GAGATGAGAT GAATGCACAC TTCCCTCAGA GTGAGCTGAG CAGAGCTGAG 
GCCTACACAC TAGTGAGCAC AGACCAGCAG TACTTGGTGC CAAAAGATGG GAAACCATTG 
GCAGGCTTGA TTCAAGATCA CATGGTTTCT GGCACCAGCA TGACCATCCG TGGCTGTTTC 
TTCACGAGGG ATCAGTACAC TGCACTAGTC TACAGAGGCC TGACAGATAA GATCGGCAGA 
GTCAAGCTTC TCCCTCCCAC ACTCATTAAG CCTGTGCTGC TGTGGACAGG AAAGCAGGTG 
GTCTCGACCC TTTTGCTGAA TGTCATCCCT GAAAACAGCA TTCCTTTAAA CTTGACTGGA 
AAAGCAAAGA TTCCTGGTAA AGCCTGGGTG AAGGAATCTC CCAGACCTGT TCCAGGATAC 
GTGCCAGAAT CCATGTGTGA ATCTCAGGTG GTCATTCGTC AGGGAGAGCT CTTGATGGGG 
GTTCTGGATA AAGCTCACTA TGGGAGCTCA GCGTATGGTC TGGTCCACTG CTGCTATGAA 
CTCTATGGAG GAGAGACTAG TGGGAAACTG CTTAGCTGTC TGGCACGGCT CTTCACTGCC 
TATCTGCAGC TTTACCGCGG GTTTACTTTG GGTGTGGAGG ACATTCTTGT AAAGGATGGA 
GCCAACAAAC AAAGAAAAAA AATCATCAAA AAGTCAGTCA CATGTGGCTC CAAGGCTCTT 
CAGGCTGCTT TCAACCTGCC TGCTTCTGCT GAAGATACTG AAGCCAGAGA ACGCTGGCAA 
GATGCACATC TGAACATCGA CCAGAGAGAT TTCAACATGG TAGACCTAAA GTTTAAAGAA 
GAGGTCAATC AAGTGAACAA CAACATCAAC AAGGTCTGCA TGCCACTGGG TCTCCACCGC 
TCGTTCCCTG AAAATAACCT ACAAATGATG GTGCAGTCAG GAGCCAAGGG GTCTACTGTT 
AATACTATGC AGATATCCTG TTTGTTGGGT CAAATTGAGC TGGAAGGCCG CCGACCACCA 
CTGATGCCTT CGGGGAAGTC TCTACCCTGC TTTCAGCCCT ATGAGCCACA GCCTCGCTCT 
GGAGGATTTG TGACTGGCAG ATTTCTGACT GGAATCAAAC CTCCTGAATT CTTCTTTCAT 
TGTATGGCTG GTCGTGAAGG TCTTGTGGAC ACTGCAGTGA AAACCAGTCG ATCTGGTTAC 
CTGCAAAGGT GTGTGATTAA GCATCTTGAA GGTTTGGTGG TCCAGTATGA TCTGACTGTG 
CGGGACAGCG ATGGCAGTGT GGTTCAGTTT CTTTATGGAG AGGATGGGTT AGACATTCCC 
AAAACACAAT TCCTACAGCC ACGACAGTTC CCTTTCATCA AAGACAACTA TGAGGTTATC 
AGGAAGTCCA AGTGCTTGGA TGAAGTTTTG GCTAAAATGG ACCCAGTGGC TGCATTAAAT 
CACTTTAAAG CTATAAAGAA ATGGAAAGCC AAGCATGAGC ATGCTTCACC CAGAGATGGT 
GCCTTCTTAC TCTTCTCCCA GAAGAAGATG AGCAAGTTGA AGGCTCAAGT AGAGGGTCAT 
GAGCTAGTCA ATGGGAGAGA GGAGGCCACT TTACAGCTCA TAGAAAGATG GAGAGCTCTG 
GATGAGTCTG GCCGGATGAG GTATTCAAAG AAGACTTCTC ATTGTCCAGA ACCGTGTCTG 
GGTCTTTATC GGCCAGACAT CAGCTTTGGT TCAGTGTCTG AGACATTTGA CTCCATTGTT 
GAGTCTTATC TTAATCAGTT CAACATCAAG CAGGAATTTT CGGCATCTTC TGAAACCACT 
GACGCAGATA GGTTGAGAAA TCTCCTGCAG CTGAAATGGC AGAGAGCTCT GTGTGACCCT 
GGAGAGGCTG TGGGTCTGGT TGCAGCTCAG AGCATCGGTG AACCATCCAC ACAAATGACC 
CTCAACACCT TTCACTTTGC CGGCAGAGGA GAGATGAATG TCACACTGGG TATTCCCAGA 
TTAAGAGAAA TTCTCTTGGT TGCGAGTTCT AACATCAAAA CTCCCATGAT GAGTATTCCA 
GTTGTAATGA ACAAGAAAGC CTTGAAGCAG GTTAAAACAC TGCGCAAGAA GATAACGCGA 
GTCTGCTTAT CAGAGGTGCT ACATAAAGTA AATGTGATAG AATCTTTCCG AATGCCGAAG 
AAGGAGACCC TGAAGTCACG AGTCTTCAAA ATCACGTTCC ACTTCCTGCC ACCTGAGCGA 
TACCAGCAAG ACAAGCTACT CAACCCTGAT GAGATCCTTC ACTACATGGA ACAAACTTTC 
TTCCGCATGC TGTTGGAGGC CATTAAGAAG CTGACTGCAA AATTGGCTGC CATCAGTTTG 
GTGGAAACTC GTAGAGCCAC AGTGAATGAT AATGATCGGG ATACCAGTGA TGGCGGCGCA 
GACAGAGAGG ACAATGGGGG AGAGGATGCA GAAAACGAGA GGATTGTGGA TGATGAGGCC 
AATGAAGGTG ATGCTGATGC CACAGATTCC AAGAGAAAAG AAAAGCAAGA AGAGGAGGTA 
GACTATGAGA GCGAGGAGGG AGAGGAAGGC AGTGATCAGG AGCAGGAGGA AGTGGCTGAG 
GAACAGGAGG CGTCTCAGGA GGTCTCTGAG GAAGGGTCTA CTGAGAGCCA ACAGAGAGTT 
AACTCTGAAC AGCCGAAGGG AAGCTCACAG GGCTCAGTGA GGATCAACTC TGTGCTGCAG 
TTAAGCGCTG CTATTGAGGA CTACAAATAT GACACCAAAA AAGGACTGTG GTGTGAGTTA 
ACCCTGGTCA TGCCGATCAC TAAAGTGCAC TTTGACCTCA CCTCAGTGGT GGTGAAACAG 
GCTCAGAACG CGGTCATCAT GGAGACCAAG GGCATCACGC GCTGTTTGTT AAATGAAGTG 
ACCAGAAAAG ACGGAAGCAA AGAGATTATG CTCAACACAG AAGGCATCAA CATTCATGAG 
ATGTTCAAAT ATGCTGATAT CCTGGACTTG AAGAGATTAT ACTCCAATGA GGTTCATGCC 
ATGGCCAGCA CATATGGTAT TGAGGTGGCC CTTAGAGTGA TCGAGAAGGA GATTAAAGAT 
GTGTTTGCTG TATACGGTAT CGAAGTGGAT CCTCGTCATC TGTCTTTAGT TGCTGATTAC 
ATGTGTTTTG AGGGAGTGTA CAAGCCTCTC AATCGTTTCG GCATTCAGTC CAACAGCTCC 
CCACTGCAAC AGATGACCTT TGAGACCAGC TTCAAGTTCC TGAAGCAAGC CACGATGTTA 
GGTTCTTATG ATAAACTCAG TTCGCCCTCA GCTTGTTTGG TTGTAGGAAA AGTTGTGAAG 
GGAGGAACTG GAATATTTGA TCTGAAACAG CCATTACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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polr1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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