Polr1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr1a
Entrez Gene ID	83581
Full Name	RNA polymerase I subunit A
Synonyms	Rpa1,Rpo1-4
General protein information	Preferred Names RNA polymerase I subunit A Names DNA-directed RNA polymerase I subunit RPA1 A194 DNA-directed RNA polymerase I A DNA-directed RNA polymerase I largest subunit DNA-directed RNA polymerase I subunit A RNA polymerase 1-4 RNA polymerase I 194 kDa subunit RNA polymerase I subunit A1 RPA194 polymerase (RNA) I polypeptide A polymerase (RNA) I subunit A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	subunit of RNA polymerase I [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762998.2	XP_008761220.2	DNA-directed RNA polymerase I subunit RPA1 isoform X1
NM_031772.1	NP_113960.1	DNA-directed RNA polymerase I subunit RPA1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
Macaca mulatta (Rhesus monkey)	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Neurospora crassa	NCU01638	XP_956006.2
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3

Gene Ontology
Bibliography

Related articles in PubMed

The Warsaw breakage syndrome-related protein DDX11 is required for ribosomal RNA synthesis and embryonic development.
Sun X, Chen H, Deng Z, Hu B, Luo H, Zeng X, Han L, Cai G, Ma L
Human molecular genetics24(17)4901-15(2015 Sep)

CRM1 and its ribosome export adaptor NMD3 localize to the nucleolus and affect rRNA synthesis.
Bai B, Moore HM, Laiho M
Nucleus (Austin, Tex.)4(4)315-25(2013 Jul-Aug)

Conservation between the RNA polymerase I, II, and III transcription initiation machineries.
Vannini A, Cramer P
Molecular cell45(4)439-46(2012 Feb)

RNA polymerase I activity is regulated at multiple steps in the transcription cycle: recent insights into factors that influence transcription elongation.
Schneider DA
Gene493(2)176-84(2012 Feb)

Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.
Yamamoto K, Yamamoto M, Hanada K, Nogi Y, Matsuyama T, Muramatsu M
Molecular and cellular biology24(14)6338-49(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08695	NM_031772.1 Latest version!	Rattus norvegicus RNA polymerase I subunit A (Polr1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa61024	XM_008762998.2 Latest version!	Rattus norvegicus polymerase (RNA) I subunit A (Polr1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr1a ( NM_031772.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113960.1 Rattus norvegicus RNA polymerase I subunit A (Polr1a), mRNA.

Price & Availability↑

CloneID	ORa08695
Clone ID Related Accession (Same CDS sequence)	NM_031772.1
Accession Version	NM_031772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Rattus norvegicus(Norway rat)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF025425.1. ##Evidence-Data-START## Transcript exon combination :: AF025425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGCCT CCAAGCACAC GCCATGGCGG CGGCTTCAGG GCATTTCCTT CGGGATGTAT 
TCGGCCGAGG AGCTCAAGAA GTTAAGTGTT AAGTCCATTA CAAATCCTCG CTATGTGGAC 
AGCCTGGGAA ACCCTTCGGC AGATGGCCTG TATGATTTGG CTTTGGGTCC TGCAGATTCC 
AAAGAAGTAT GCTCTACCTG TGTGCAGGAC TTCAACAACT GCTCCGGACA CCTGGGCCAC 
ATTGACCTCC CGCTCACAGT GTACAACCCT CTCCTCTTCG ATAAACTCTA CCTGCTGCTG 
CGAGGCTCCT GTTTAAATTG TCATATGCTG ACTTGTCCTC GGGCTGCAAT TCACCTATTG 
GTCTGCCAGC TGAAGGTCCT AGACGTTGGA GCCCTACAAG CAGTCTATGA GCTTGAGCGG 
ATTCTCAGCA GGTTTCTGGA AGAAACTTCG GATCCTTCTG CCTTCGAAAT TCAAGAAGAG 
TTAGAAGAGT ACACAAGCAA AATTCTGCAG AATAACCTCC TGGGGTCCCA GGGTGCACAT 
GTGAAAAATG TGTGCGAGAG CAGAAGCAAA CTTGTCGCTC ACTTCTGGAA GACACACATG 
GCTGCTAAGC GCTGTCCACA CTGCAAGACG GGACGGTCAG TGGTTCGAAA GGAGCACAAT 
AGTAAGCTAA CAATAACATA TCCAGCCATG GTGCACAAGA AGTCTGGCCA GAAGGATGCA 
GAACTCCCAG AAGGAGCACC AGCAGCTCCA GGAATTGATG AGGCTCAGAT GGGGAAACGA 
GGATACTTGA CCCCAAGCAG CGCCCAAGAA CATCTTTTCG CCATTTGGAA GAATGAAGGA 
TTCTTCCTGA ATTATCTTTT TTCGGGATTG GATGATATTG GCCCAGAGTC CAGTTTCAAC 
CCCAGTATGT TCTTTCTAGA TTTCATCGTG GTACCGCCAT CCAGGTATCG TCCCATCAAT 
CGCCTGGGTG ACCAGATGTT CACTAATGGC CAGACAGTGA ATTTGCAAGC GGTCATGAAG 
GATGCAGTTT TGATCCGGAA ACTTCTGGCA GTGATGGCCC AAGAACAGAA ACTGCCCTGT 
GAGATGACAG AAATCACCAT AGACAAGGAA AATGACTCCT CGGGTGCTAT CGACCGGTCC 
TTTCTGAGTT TGCTTCCAGG ACAGTCTCTC ACAGATAAAC TTTACAATAT TTGGATTCGC 
CTCCAGAGCC ACGTCAATAT TGTGTTTGAC AGTGACATGG ACAAACTGAT GCTGGAAAAG 
TACCCTGGCA TAAGACAGAT CCTCGAGAAG AAGGAAGGCC TGTTCCGGAA GCACATGATG 
GGAAAGCGAG TGGACTACGC AGCCCGCTCG GTCATCTGCC CAGATATGTA CATCAACACC 
AATGAGATCG GGATTCCCAT GGTATTTGCC ACAAAACTGA CTTACCCCCA GCCAGTTACC 
CCCTGGAACG TGCAGGAACT GAGACAGGCA GTCATCAATG GACCCAATGT GCACCCAGGA 
GCCTCCATGG TCATCAACGA GGATGGCAGT CGCACAGCCC TGAGCGCAGT GGATGCTACA 
CAGCGGGAAG CTGTGGCCAA ACAGCTCCTG ACACCGTCCA CAGGGATCCC TAAGCCTCAG 
GGAGCAAAAG TTGTGTGCCG GCATGTGAAG AATGGGGACA TTCTCCTCCT GAACCGACAG 
CCCACCTTGC ACAGACCCTC CATCCAGGCC CACCGTGCCC ACATTCTGCC TGAGGAAAAA 
GTCCTGCGCC TTCACTATGC CAACTGCAAG GCCTACAATG CTGACTTCGA TGGAGACGAG 
ATGAATGCCC ACTTCCCCCA GAGTGAGCTG GGCCGAGCTG AAGCCTATGT CCTGGCCTGC 
ACTGACCAGC AGTATCTTGT TCCCAAGGAT GGCCAGCCCT TGGCAGGACT GATTCAGGAT 
CACATGGTGT CGGGTGCAAA CATGACCATT CGGGGATGCT TTTTCACCCG GGAACAGTAC 
ATGGAGCTGG TGTACCGAGG GCTCACAGAC AAAGTGGGAC GCGTGAAGCT CTTTCCTCCT 
GCCATCCTGA AGCCCTTCCC GCTGTGGACA GGAAAGCAGG TCGTGTCGAC CCTCCTCATA 
AACATAATCC CAGAGGACTA CACCCCCCTG AATTTAACTG GAAAGGCAAA AATCGGCAGT 
AAAGCCTGGG TGAAGGAAAA GCCTCGGCCT GTTCCTGACT TTGACCCTGA CTCCATGTGT 
GAGTCCCAGG TGATCATCAG GGAAGGGGAG CTGCTCTGCG GGGTGCTGGA CAAGGCACAC 
TATGGGAGCT CTGCCTACGG CCTGGTCCAC TGCTGCTACG AGATCTATGG GGGTGAGACC 
AGCGGCAGGG TTCTCACCTG CTTAGCCCGC CTCTTCACTG CCTACCTACA GCTCTATCGA 
GGTTTCACCT TGGGTGTGGA AGACATTTTG GTTAAGCCAA ATGCAGATGT GATGAGACAG 
CGCATCATTG AAGAATCTAC CCAGTGTGGA CCCCGGGCTG TCAGGGCTGC GTTGAACCTG 
CCAGAGGCTG CATCATGTGA TGAGATCCAG GGGAAATGGC AGGATGCCAT CTGGCGCAAA 
GACCAGAGGG ATTTTAACAT GATTGATATG AAGTTCAAGG AGGAAGTGAA CCATTACAGC 
AATGAGATTA ACAAGGCATG TATGCCTTTT GGCTTGCACA GACAATTCCC TGAAAACAAC 
CTGCAGATGA TGGTGCAGTC GGGAGCCAAA GGGTCGACCG TGAACACGAT GCAAATCTCT 
TGCCTGCTGG GCCAAATTGA GTTGGAAGGT CGGAGACCCC CACTGATGGC GTCTGGCAAG 
TCACTGCCCT GCTTTGAGCC TTACGAGTTC ACCCCAAGGG CTGGTGGCTT TGTCACTGGA 
AGGTTCCTTA CCGGTATCAG GCCACCTGAG TTCTTCTTCC ACTGCATGGC GGGGCGAGAA 
GGCCTGGTGG ATACTGCTGT CAAGACCAGC CGTTCTGGCT ATCTCCAAAG GTGTATCATC 
AAGCACCTGG AGGGTCTGGT CATCCAGTAC GATCTCACGG TCCGTGACAG TGATGGCAGT 
GTGGTACAGT TCCTGTATGG GGAGGATGGC CTGGACATCC CCAAGACACA GTTCCTACAA 
CCCAAGCAGT TCCCCTTTCT GGCCAGCAAT TATGAGGTGA TAATGAAATC CAAGCATCTT 
CATGAAGTTT TGTCTAGAGC AGATCCCCAG AAAGTGCTCC GCCACTTCAG AGCCATTAAA 
AAATGGCACC ACAGGCATTC CAGTGCCCTG CTGAGAAAAG GCGCCTTCCT GAGTTTTTCC 
CAGAAGATTC AGGCAGCTGT GAAAGCCTTG AACCTAGAGG GCAAGACCCA GAATGGGCGC 
AGCCCAGAGA CCCAGCAGAT GTTACAGATG TGGCATGAAT TAGATGAGCA GAGCCGACGG 
AAATACCAGA AGAGGGCTGC CCCGTGTCCC GACCCTAGCC TTTCTGTGTG GCGCCCAGAC 
ATCCACTTCG CATCTGTGTC TGAAACATTT GAAAAAAAGA TTGATGACTA CAGTCAGGAG 
TGGGCAGCTC AAGCAGAGAA GAGTCACAAC AGATCAGAGC TTTCTCTTGA CAGGCTGAGA 
ACCTTACTGC AGCTCAAGTG GCAGCGCTCA CTGTGTGACC CAGGCGAGGC TGTTGGCCTC 
CTGGCCGCCC AGAGCATCGG AGAGCCCTCC ACACAGATGA CACTGAACAC CTTCCACTTT 
GCAGGCAGAG GCGAGATGAA CGTCACCCTG GGCATTCCAA GATTGAGGGA GATTCTCATG 
GTGGCCAGTG CCAACATCAA AACACCCATG ATGAGCGTGC CTGTGTTCAA CACCAAGAAA 
GCACTGAGGA GAGTGAAGAG TCTGAAGAAG CAGCTCACCA GGGTGTGCCT GGGGGAGGTA 
TTGCAGAAAG TTGACATCCA GGAGTCCTTC TGTATGGGAG AGAAACAGAA TAAATTCCGG 
GTGTATGAGC TCCGGTTTCA GTTCCTGCCA CATGCATATT ACCAGCAGGA GAAGTGCCTG 
AGACCCGAGG ACATCTTGCA CTTCATGGAA ACCAGATTCT TTAAGCTACT AATGGAAGCT 
ATCAAAAAGA AGAATAGCAA AGCATCAGCC TTCAGAAGTG TGAATACCAG GAGAGCTACA 
CAGAAGGACC TCGATGACAC TGAGGACTCG GGGAGGAATC GGAGAGAGGA GGAAAGGGAT 
GAGGAAGAGG AGGGGAACAT TGTGGATGCT GAAGCTGAGG AGGGGGATGC AGATGCTTCT 
GACACCAAAC GCAAGGAGAA GCAAGAAGAA GAGGTTGACT ATGAGAGTGA GGAAGAAGGG 
GAGGAGGAAG AGGAAGAGGA TGTACAAGAA GAGGAGAACA TCAAAGGTGA AGGTGCCCAC 
CAGACCCATG AGCCAGATGA GGAAGAAGGT TCAGGCCTTG AGGAGGAATC ATCCCAGAAC 
CCTCCCTGCC GCCATTCCAG GCCTCAGGGA GCTGAGGCCA TGGAACGCCG AATCCAGGCT 
GTACGGGAGT CCCATTCGTT CATTGAGGAC TACCAGTATG ACACAGAGGA GAGCCTGTGG 
TGCCAGGTGA CAGTGAAGCT CCCATTGATG AAGATTAATT TTGACATGAG CTCCTTGGTT 
GTATCCTTGG CCCACAATGC CATTGTCTAT ACGACTAAGG GCATCACTCG GTGTCTCCTG 
AACGAAACGA TCAACAGTAA GAATGAGAAG GAGTTTGTCC TGAACACGGA GGGAATCAAC 
CTCCCCGAGC TGTTCAAGTA CTCCGAGGTG CTGGACCTGC GCCGCCTGTA CTCCAACGAC 
ATCCATGCTG TGGCCAATAC GTACGGCATC GAGGCTGCGC TGCGGGTGAT AGAGAAGGAG 
ATTAAGGATG TGTTTGCTGT GTACGGCATT GCGGTTGATC CTCGCCATCT CTCCCTGGTT 
GCTGATTATA TGTGTTTTGA GGGTGTTTAT AAGCCACTCA ATCGCTTTGG GATCCAGTCA 
AGCTCCTCCC CTTTGCAGCA GATGACGTTT GAAACCAGCT TCCAGTTTCT GAAACAGGCC 
ACCATGATGG GATCCCATGA TGAACTGAAG TCACCTTCTG CCTGCCTTGT GGTGGGAAAG 
GTCGTCAAAG GCGGGACAGG CCTGTTTGAG CTCAAGCAGC CCCTAAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113960.1
CDS	57..5207
Translation	MLASKHTPWRRLQGISFGMYSAEELKKLSVKSITNPRYVDSLGNPSADGLYDLALGPADSKEVCSTCVQDFNNCSGHLGHIDLPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAAIHLLVCQLKVLDVGALQAVYELERILSRFLEETSDPSAFEIQEELEEYTSKILQNNLLGSQGAHVKNVCESRSKLVAHFWKTHMAAKRCPHCKTGRSVVRKEHNSKLTITYPAMVHKKSGQKDAELPEGAPAAPGIDEAQMGKRGYLTPSSAQEHLFAIWKNEGFFLNYLFSGLDDIGPESSFNPSMFFLDFIVVPPSRYRPINRLGDQMFTNGQTVNLQAVMKDAVLIRKLLAVMAQEQKLPCEMTEITIDKENDSSGAIDRSFLSLLPGQSLTDKLYNIWIRLQSHVNIVFDSDMDKLMLEKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDATQREAVAKQLLTPSTGIPKPQGAKVVCRHVKNGDILLLNRQPTLHRPSIQAHRAHILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYTPLNLTGKAKIGSKAWVKEKPRPVPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVMRQRIIEESTQCGPRAVRAALNLPEAASCDEIQGKWQDAIWRKDQRDFNMIDMKFKEEVNHYSNEINKACMPFGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIMKSKHLHEVLSRADPQKVLRHFRAIKKWHHRHSSALLRKGAFLSFSQKIQAAVKALNLEGKTQNGRSPETQQMLQMWHELDEQSRRKYQKRAAPCPDPSLSVWRPDIHFASVSETFEKKIDDYSQEWAAQAEKSHNRSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFNTKKALRRVKSLKKQLTRVCLGEVLQKVDIQESFCMGEKQNKFRVYELRFQFLPHAYYQQEKCLRPEDILHFMETRFFKLLMEAIKKKNSKASAFRSVNTRRATQKDLDDTEDSGRNRREEERDEEEEGNIVDAEAEEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEDVQEEENIKGEGAHQTHEPDEEEGSGLEEESSQNPPCRHSRPQGAEAMERRIQAVRESHSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHNAIVYTTKGITRCLLNETINSKNEKEFVLNTEGINLPELFKYSEVLDLRRLYSNDIHAVANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPSACLVVGKVVKGGTGLFELKQPLR

Target ORF information:

RefSeq Version	NM_031772.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA polymerase I subunit A (Polr1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031772.1

ORF Insert Sequence:

ATGCTGGCCT CCAAGCACAC GCCATGGCGG CGGCTTCAGG GCATTTCCTT CGGGATGTAT 
TCGGCCGAGG AGCTCAAGAA GTTAAGTGTT AAGTCCATTA CAAATCCTCG CTATGTGGAC 
AGCCTGGGAA ACCCTTCGGC AGATGGCCTG TATGATTTGG CTTTGGGTCC TGCAGATTCC 
AAAGAAGTAT GCTCTACCTG TGTGCAGGAC TTCAACAACT GCTCCGGACA CCTGGGCCAC 
ATTGACCTCC CGCTCACAGT GTACAACCCT CTCCTCTTCG ATAAACTCTA CCTGCTGCTG 
CGAGGCTCCT GTTTAAATTG TCATATGCTG ACTTGTCCTC GGGCTGCAAT TCACCTATTG 
GTCTGCCAGC TGAAGGTCCT AGACGTTGGA GCCCTACAAG CAGTCTATGA GCTTGAGCGG 
ATTCTCAGCA GGTTTCTGGA AGAAACTTCG GATCCTTCTG CCTTCGAAAT TCAAGAAGAG 
TTAGAAGAGT ACACAAGCAA AATTCTGCAG AATAACCTCC TGGGGTCCCA GGGTGCACAT 
GTGAAAAATG TGTGCGAGAG CAGAAGCAAA CTTGTCGCTC ACTTCTGGAA GACACACATG 
GCTGCTAAGC GCTGTCCACA CTGCAAGACG GGACGGTCAG TGGTTCGAAA GGAGCACAAT 
AGTAAGCTAA CAATAACATA TCCAGCCATG GTGCACAAGA AGTCTGGCCA GAAGGATGCA 
GAACTCCCAG AAGGAGCACC AGCAGCTCCA GGAATTGATG AGGCTCAGAT GGGGAAACGA 
GGATACTTGA CCCCAAGCAG CGCCCAAGAA CATCTTTTCG CCATTTGGAA GAATGAAGGA 
TTCTTCCTGA ATTATCTTTT TTCGGGATTG GATGATATTG GCCCAGAGTC CAGTTTCAAC 
CCCAGTATGT TCTTTCTAGA TTTCATCGTG GTACCGCCAT CCAGGTATCG TCCCATCAAT 
CGCCTGGGTG ACCAGATGTT CACTAATGGC CAGACAGTGA ATTTGCAAGC GGTCATGAAG 
GATGCAGTTT TGATCCGGAA ACTTCTGGCA GTGATGGCCC AAGAACAGAA ACTGCCCTGT 
GAGATGACAG AAATCACCAT AGACAAGGAA AATGACTCCT CGGGTGCTAT CGACCGGTCC 
TTTCTGAGTT TGCTTCCAGG ACAGTCTCTC ACAGATAAAC TTTACAATAT TTGGATTCGC 
CTCCAGAGCC ACGTCAATAT TGTGTTTGAC AGTGACATGG ACAAACTGAT GCTGGAAAAG 
TACCCTGGCA TAAGACAGAT CCTCGAGAAG AAGGAAGGCC TGTTCCGGAA GCACATGATG 
GGAAAGCGAG TGGACTACGC AGCCCGCTCG GTCATCTGCC CAGATATGTA CATCAACACC 
AATGAGATCG GGATTCCCAT GGTATTTGCC ACAAAACTGA CTTACCCCCA GCCAGTTACC 
CCCTGGAACG TGCAGGAACT GAGACAGGCA GTCATCAATG GACCCAATGT GCACCCAGGA 
GCCTCCATGG TCATCAACGA GGATGGCAGT CGCACAGCCC TGAGCGCAGT GGATGCTACA 
CAGCGGGAAG CTGTGGCCAA ACAGCTCCTG ACACCGTCCA CAGGGATCCC TAAGCCTCAG 
GGAGCAAAAG TTGTGTGCCG GCATGTGAAG AATGGGGACA TTCTCCTCCT GAACCGACAG 
CCCACCTTGC ACAGACCCTC CATCCAGGCC CACCGTGCCC ACATTCTGCC TGAGGAAAAA 
GTCCTGCGCC TTCACTATGC CAACTGCAAG GCCTACAATG CTGACTTCGA TGGAGACGAG 
ATGAATGCCC ACTTCCCCCA GAGTGAGCTG GGCCGAGCTG AAGCCTATGT CCTGGCCTGC 
ACTGACCAGC AGTATCTTGT TCCCAAGGAT GGCCAGCCCT TGGCAGGACT GATTCAGGAT 
CACATGGTGT CGGGTGCAAA CATGACCATT CGGGGATGCT TTTTCACCCG GGAACAGTAC 
ATGGAGCTGG TGTACCGAGG GCTCACAGAC AAAGTGGGAC GCGTGAAGCT CTTTCCTCCT 
GCCATCCTGA AGCCCTTCCC GCTGTGGACA GGAAAGCAGG TCGTGTCGAC CCTCCTCATA 
AACATAATCC CAGAGGACTA CACCCCCCTG AATTTAACTG GAAAGGCAAA AATCGGCAGT 
AAAGCCTGGG TGAAGGAAAA GCCTCGGCCT GTTCCTGACT TTGACCCTGA CTCCATGTGT 
GAGTCCCAGG TGATCATCAG GGAAGGGGAG CTGCTCTGCG GGGTGCTGGA CAAGGCACAC 
TATGGGAGCT CTGCCTACGG CCTGGTCCAC TGCTGCTACG AGATCTATGG GGGTGAGACC 
AGCGGCAGGG TTCTCACCTG CTTAGCCCGC CTCTTCACTG CCTACCTACA GCTCTATCGA 
GGTTTCACCT TGGGTGTGGA AGACATTTTG GTTAAGCCAA ATGCAGATGT GATGAGACAG 
CGCATCATTG AAGAATCTAC CCAGTGTGGA CCCCGGGCTG TCAGGGCTGC GTTGAACCTG 
CCAGAGGCTG CATCATGTGA TGAGATCCAG GGGAAATGGC AGGATGCCAT CTGGCGCAAA 
GACCAGAGGG ATTTTAACAT GATTGATATG AAGTTCAAGG AGGAAGTGAA CCATTACAGC 
AATGAGATTA ACAAGGCATG TATGCCTTTT GGCTTGCACA GACAATTCCC TGAAAACAAC 
CTGCAGATGA TGGTGCAGTC GGGAGCCAAA GGGTCGACCG TGAACACGAT GCAAATCTCT 
TGCCTGCTGG GCCAAATTGA GTTGGAAGGT CGGAGACCCC CACTGATGGC GTCTGGCAAG 
TCACTGCCCT GCTTTGAGCC TTACGAGTTC ACCCCAAGGG CTGGTGGCTT TGTCACTGGA 
AGGTTCCTTA CCGGTATCAG GCCACCTGAG TTCTTCTTCC ACTGCATGGC GGGGCGAGAA 
GGCCTGGTGG ATACTGCTGT CAAGACCAGC CGTTCTGGCT ATCTCCAAAG GTGTATCATC 
AAGCACCTGG AGGGTCTGGT CATCCAGTAC GATCTCACGG TCCGTGACAG TGATGGCAGT 
GTGGTACAGT TCCTGTATGG GGAGGATGGC CTGGACATCC CCAAGACACA GTTCCTACAA 
CCCAAGCAGT TCCCCTTTCT GGCCAGCAAT TATGAGGTGA TAATGAAATC CAAGCATCTT 
CATGAAGTTT TGTCTAGAGC AGATCCCCAG AAAGTGCTCC GCCACTTCAG AGCCATTAAA 
AAATGGCACC ACAGGCATTC CAGTGCCCTG CTGAGAAAAG GCGCCTTCCT GAGTTTTTCC 
CAGAAGATTC AGGCAGCTGT GAAAGCCTTG AACCTAGAGG GCAAGACCCA GAATGGGCGC 
AGCCCAGAGA CCCAGCAGAT GTTACAGATG TGGCATGAAT TAGATGAGCA GAGCCGACGG 
AAATACCAGA AGAGGGCTGC CCCGTGTCCC GACCCTAGCC TTTCTGTGTG GCGCCCAGAC 
ATCCACTTCG CATCTGTGTC TGAAACATTT GAAAAAAAGA TTGATGACTA CAGTCAGGAG 
TGGGCAGCTC AAGCAGAGAA GAGTCACAAC AGATCAGAGC TTTCTCTTGA CAGGCTGAGA 
ACCTTACTGC AGCTCAAGTG GCAGCGCTCA CTGTGTGACC CAGGCGAGGC TGTTGGCCTC 
CTGGCCGCCC AGAGCATCGG AGAGCCCTCC ACACAGATGA CACTGAACAC CTTCCACTTT 
GCAGGCAGAG GCGAGATGAA CGTCACCCTG GGCATTCCAA GATTGAGGGA GATTCTCATG 
GTGGCCAGTG CCAACATCAA AACACCCATG ATGAGCGTGC CTGTGTTCAA CACCAAGAAA 
GCACTGAGGA GAGTGAAGAG TCTGAAGAAG CAGCTCACCA GGGTGTGCCT GGGGGAGGTA 
TTGCAGAAAG TTGACATCCA GGAGTCCTTC TGTATGGGAG AGAAACAGAA TAAATTCCGG 
GTGTATGAGC TCCGGTTTCA GTTCCTGCCA CATGCATATT ACCAGCAGGA GAAGTGCCTG 
AGACCCGAGG ACATCTTGCA CTTCATGGAA ACCAGATTCT TTAAGCTACT AATGGAAGCT 
ATCAAAAAGA AGAATAGCAA AGCATCAGCC TTCAGAAGTG TGAATACCAG GAGAGCTACA 
CAGAAGGACC TCGATGACAC TGAGGACTCG GGGAGGAATC GGAGAGAGGA GGAAAGGGAT 
GAGGAAGAGG AGGGGAACAT TGTGGATGCT GAAGCTGAGG AGGGGGATGC AGATGCTTCT 
GACACCAAAC GCAAGGAGAA GCAAGAAGAA GAGGTTGACT ATGAGAGTGA GGAAGAAGGG 
GAGGAGGAAG AGGAAGAGGA TGTACAAGAA GAGGAGAACA TCAAAGGTGA AGGTGCCCAC 
CAGACCCATG AGCCAGATGA GGAAGAAGGT TCAGGCCTTG AGGAGGAATC ATCCCAGAAC 
CCTCCCTGCC GCCATTCCAG GCCTCAGGGA GCTGAGGCCA TGGAACGCCG AATCCAGGCT 
GTACGGGAGT CCCATTCGTT CATTGAGGAC TACCAGTATG ACACAGAGGA GAGCCTGTGG 
TGCCAGGTGA CAGTGAAGCT CCCATTGATG AAGATTAATT TTGACATGAG CTCCTTGGTT 
GTATCCTTGG CCCACAATGC CATTGTCTAT ACGACTAAGG GCATCACTCG GTGTCTCCTG 
AACGAAACGA TCAACAGTAA GAATGAGAAG GAGTTTGTCC TGAACACGGA GGGAATCAAC 
CTCCCCGAGC TGTTCAAGTA CTCCGAGGTG CTGGACCTGC GCCGCCTGTA CTCCAACGAC 
ATCCATGCTG TGGCCAATAC GTACGGCATC GAGGCTGCGC TGCGGGTGAT AGAGAAGGAG 
ATTAAGGATG TGTTTGCTGT GTACGGCATT GCGGTTGATC CTCGCCATCT CTCCCTGGTT 
GCTGATTATA TGTGTTTTGA GGGTGTTTAT AAGCCACTCA ATCGCTTTGG GATCCAGTCA 
AGCTCCTCCC CTTTGCAGCA GATGACGTTT GAAACCAGCT TCCAGTTTCT GAAACAGGCC 
ACCATGATGG GATCCCATGA TGAACTGAAG TCACCTTCTG CCTGCCTTGT GGTGGGAAAG 
GTCGTCAAAG GCGGGACAGG CCTGTTTGAG CTCAAGCAGC CCCTAAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr1a ( XM_008762998.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761220.2 Rattus norvegicus polymerase (RNA) I subunit A (Polr1a), transcript variant X1, mRNA.

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CloneID	ORa61024
Clone ID Related Accession (Same CDS sequence)	XM_008762998.2
Accession Version	XM_008762998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA-directed RNA polymerase I subunit RPA1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCCT CCAAGCACAC GCCATGGCGG CGGCTTCAGG GCATTTCCTT CGGGATGTAT 
TCGGCCGAGG AGCTCAAGAA GTTAAGTGTT AAGTCCATTA CAAATCCTCG CTATGTGGAC 
AGCCTGGGAA ACCCTTCGGC AGATGGCCTG TATGATTTGG CTTTGGGTCC TGCAGATTCC 
AAAGAAGTAT GCTCTACCTG TGTGCAGGAC TTCAACAACT GCTCCGGACA CCTGGGCCAC 
ATTGACCTCC CGCTCACAGT GTACAACCCT CTCCTCTTCG ATAAACTCTA CCTGCTGCTG 
CGAGGCTCCT GTTTAAATTG TCATATGCTG ACTTGTCCTC GGGCTGCAAT TCACCTATTG 
GTCTGCCAGC TGAAGGTCCT AGACGTTGGA GCCCTACAAG CAGTCTATGA GCTTGAGCGG 
ATTCTCAGCA GGTTTCTGGA AGAAACTTCG GATCCTTCTG CCTTCGAAAT TCAAGAAGAG 
TTAGAAGAGT ACACAAGCAA AATTCTGCAG AATAACCTCC TGGGGTCCCA GGGTGCACAT 
GTGAAAAATG TGTGCGAGAG CAGAAGCAAA CTTGTCGCTC ACTTCTGGAA GACACACATG 
GCTGCTAAGC GCTGTCCACA CTGCAAGACG GGACGGTCAG TGGTTCGAAA GGAGCACAAT 
AGTAAGCTAA CAATAACATA TCCAGCCATG GTGCACAAGA AGTCTGGCCA GAAGGATGCA 
GAACTCCCAG AAGGAGCACC AGCAGCTCCA GGAATTGATG AGGCTCAGAT GGGGAAACGA 
GGATACTTGA CCCCAAGCAG CGCCCAAGAA CATCTTTTCG CCATTTGGAA GAATGAAGGA 
TTCTTCCTGA ATTATCTTTT TTCGGGATTG GATGATATTG GCCCAGAGTC CAGTTTCAAC 
CCCAGTATGT TCTTTCTAGA TTTCATCGTG GTACCGCCAT CCAGGTATCG TCCCATCAAT 
CGCCTGGGTG ACCAGATGTT CACTAATGGC CAGACAGTGA ATTTGCAAGC GGTCATGAAG 
GATGCAGTTT TGATCCGGAA ACTTCTGGCA GTGATGGCCC AAGAACAGAA ACTGCCCTGT 
GAGATGACAG AAATCACCAT AGACAAGGAA AATGACTCCT CGGGTGCTAT CGACCGGTCC 
TTTCTGAGTT TGCTTCCAGG ACAGTCTCTC ACAGATAAAC TTTACAATAT TTGGATTCGC 
CTCCAGAGCC ACGTCAATAT TGTGTTTGAC AGTGACATGG ACAAACTGAT GCTGGAAAAG 
TACCCTGGCA TAAGACAGAT CCTCGAGAAG AAGGAAGGCC TGTTCCGGAA GCACATGATG 
GGAAAGCGAG TGGACTACGC AGCCCGCTCG GTCATCTGCC CAGATATGTA CATCAACACC 
AATGAGATCG GGATTCCCAT GGTATTTGCC ACAAAACTGA CTTACCCCCA GCCAGTTACC 
CCCTGGAACG TGCAGGAACT GAGACAGGCA GTCATCAATG GACCCAATGT GCACCCAGGA 
GCCTCCATGG TCATCAACGA GGATGGCAGT CGCACAGCCC TGAGCGCAGT GGATGCTACA 
CAGCGGGAAG CTGTGGCCAA ACAGCTCCTG ACACCGTCCA CAGGGATCCC TAAGCCTCAG 
GGAGCAAAAG TTGTGTGCCG GCATGTGAAG AATGGGGACA TTCTCCTCCT GAACCGACAG 
CCCACCTTGC ACAGACCCTC CATCCAGGCC CACCGTGCCC ACATTCTGCC TGAGGAAAAA 
GTCCTGCGCC TTCACTATGC CAACTGCAAG GCCTACAATG CTGACTTCGA TGGAGACGAG 
ATGAATGCCC ACTTCCCCCA GAGTGAGCTG GGCCGAGCTG AAGCCTATGT CCTGGCCTGC 
ACTGACCAGC AGTATCTTGT TCCCAAGGAT GGCCAGCCCT TGGCAGGACT GATTCAGGAT 
CACATGGTGT CGGGTGCAAA CATGACCATT CGGGGATGCT TTTTCACCCG GGAACAGTAC 
ATGGAGCTGG TGTACCGAGG GCTCACAGAC AAAGTGGGAC GCGTGAAGCT CTTTCCTCCT 
GCCATCCTGA AGCCCTTCCC GCTGTGGACA GGAAAGCAGG TCGTGTCGAC CCTCCTCATA 
AACATAATCC CAGAGGACTA CACCCCCCTG AATTTAACTG GAAAGGCAAA AATCGGCAGT 
AAAGCCTGGG TGAAGGAAAA GCCTCGGCCT GTTCCTGACT TTGACCCTGA CTCCATGTGT 
GAGTCCCAGG TGATCATCAG GGAAGGGGAG CTGCTCTGCG GGGTGCTGGA CAAGGCACAC 
TATGGGAGCT CTGCCTACGG CCTGGTCCAC TGCTGCTACG AGATCTATGG GGGTGAGACC 
AGCGGCAGGG TTCTCACCTG CTTAGCCCGC CTCTTCACTG CCTACCTACA GCTCTATCGA 
GGTTTCACCT TGGGTGTGGA AGACATTTTG GTTAAGCCAA ATGCAGATGT GATGAGACAG 
CGCATCATTG AAGAATCTAC CCAGTGTGGA CCCCGGGCTG TCAGGGCTGC GTTGAACCTG 
CCAGAGGCTG CATCATGTGA TGAGATCCAG GGGAAATGGC AGGATGCCCA TCTGGGCAAA 
GACCAGAGGG ATTTTAACAT GATTGATATG AAGTTCAAGG AGGAAGTGAA CCATTACAGC 
AATGAGATTA ACAAGGCATG TATGCCTTTT GGCTTGCACA GACAATTCCC TGAAAACAAC 
CTGCAGATGA TGGTGCAGTC GGGAGCCAAA GGGTCGACCG TGAACACGAT GCAAATCTCT 
TGCCTGCTGG GCCAAATTGA GTTGGAAGGT CGGAGACCCC CACTGATGGC GTCTGGCAAG 
TCACTGCCCT GCTTTGAGCC TTACGAGTTC ACCCCAAGGG CTGGTGGCTT TGTCACTGGA 
AGGTTCCTTA CCGGTATCAG GCCACCTGAG TTCTTCTTCC ACTGCATGGC GGGGCGAGAA 
GGCCTGGTGG ATACTGCTGT CAAGACCAGC CGTTCTGGCT ATCTCCAAAG GTGTATCATC 
AAGCACCTGG AGGGTCTGGT CATCCAGTAC GATCTCACGG TCCGTGACAG TGATGGCAGT 
GTGGTACAGT TCCTGTATGG GGAGGATGGC CTGGACATCC CCAAGACACA GTTCCTACAA 
CCCAAGCAGT TCCCCTTTCT GGCCAGCAAT TATGAGGTGA TAATGAAATC CAAGCATCTT 
CATGAAGTTT TGTCTAGAGC AGATCCCCAG AAAGTGCTCC GCCACTTCAG AGCCATTAAA 
AAATGGCACC ACAGGCATTC CAGTGCCCTG CTGAGAAAAG GCGCCTTCCT GAGTTTTTCC 
CAGAAGATTC AGGCAGCTGT GAAAGCCTTG AACCTAGAGG GCAAGACCCA GAATGGGCGC 
AGCCCAGAGA CCCAGCAGAT GTTACAGATG TGGCATGAAT TAGATGAGCA GAGCCGACGG 
AAATACCAGA AGAGGGCTGC CCCGTGTCCC GACCCTAGCC TTTCTGTGTG GCGCCCAGAC 
ATCCACTTCG CATCTGTGTC TGAAACATTT GAAAAAAAGA TTGATGACTA CAGTCAGGAG 
TGGGCAGCTC AAGCAGAGAA GAGTCACAAC AGATCAGAGC TTTCTCTTGA CAGGCTGAGA 
ACCTTACTGC AGCTCAAGTG GCAGCGCTCA CTGTGTGACC CAGGCGAGGC TGTTGGCCTC 
CTGGCCGCCC AGAGCATCGG AGAGCCCTCC ACACAGATGA CACTGAACAC CTTCCACTTT 
GCAGGCAGAG GCGAGATGAA CGTCACCCTG GGCATTCCAA GATTGAGGGA GATTCTCATG 
GTGGCCAGTG CCAACATCAA AACACCCATG ATGAGCGTGC CTGTGTTCAA CACCAAGAAA 
GCACTGAGGA GAGTGAAGAG TCTGAAGAAG CAGCTCACCA GGGTGTGCCT GGGGGAGGTA 
TTGCAGAAAG TTGACATCCA GGAGTCCTTC TGTATGGGAG AGAAACAGAA TAAATTCCGG 
GTGTATGAGC TCCGGTTTCA GTTCCTGCCA CATGCATATT ACCAGCAGGA GAAGTGCCTG 
AGACCCGAGG ACATCTTGCA CTTCATGGAA ACCAGATTCT TTAAGCTACT AATGGAAGCT 
ATCAAAAAGA AGAATAGCAA AGCATCAGCC TTCAGAAGTG TGAATACCAG GAGAGCTACA 
CAGAAGGACC TCGATGACAC TGAGGACTCG GGGAGGAATC GGAGAGAGGA GGAAAGGGAT 
GAGGAAGAGG AGGGGAACAT TGTGGATGCT GAAGCTGAGG AGGGGGATGC AGATGCTTCT 
GACACCAAAC GCAAGGAGAA GCAAGAAGAA GAGGTTGACT ATGAGAGTGA GGAAGAAGGG 
GAGGAGGAAG AGGAAGAGGA TGTACAAGAA GAGGAGAACA TCAAAGGTGA AGGTGCCCAC 
CAGACCCATG AGCCAGATGA GGAAGAAGGT TCAGGCCTTG AGGAGGAATC ATCCCAGAAC 
CCTCCCCGCC GCCATTCCAG GCCTCAGGGA GCTGAGGCCA TGGAACGCCG AATCCAGGCT 
GTACGGGAGT CCCATTCGTT CATTGAGGAC TACCAGTATG ACACAGAGGA GAGCCTGTGG 
TGCCAGGTGA CAGTGAAGCT CCCATTGATG AAGATTAATT TTGACATGAG CTCCTTGGTT 
GTATCCTTGG CCCACAATGC CATTGTCTAT ACGACTAAGG GCATCACTCG GTGTCTCCTG 
AATGAAACGA TCAACAGTAA GAATGAGAAG GAGTTTGTCC TGAACACGGA GGGAATCAAC 
CTCCCCGAGC TGTTCAAGTA CTCCGAGGTG CTGGACCTGC GCCGCCTGTA CTCCAACGAC 
ATCCATGCTG TGGCCAATAC GTACGGCATC GAGGCTGCGC TGCGGGTGAT AGAGAAGGAG 
ATTAAGGATG TGTTTGCTGT GTACGGCATT GCGGTTGATC CTCGCCATCT CTCCCTGGTT 
GCTGATTATA TGTGTTTTGA GGGTGTTTAT AAGCCACTCA ATCGCTTTGG GATCCAGTCA 
AGCTCCTCCC CTTTGCAGCA GATGACGTTT GAAACCAGCT TCCAGTTTCT GAAACAGGCC 
ACCATGATGG CCATGGCCTT GCCACCGTTG GTTCACTTCT ACAGTGAGTC AGACTATCCC 
AAGTGCTGGC AATATGAGGA TAAAGAGGCT GCTCACCTTG CAATGGACCA GAAGCAGGGA 
GGAAAGCACC GAGATCCAGC TATAACTTTC AAAGGCCCTG AGTGCCAGGC AAGCAGCTCA 
CCAGGGCAGA CCTGCAGAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761220.2
CDS	338..5581
Translation	MLASKHTPWRRLQGISFGMYSAEELKKLSVKSITNPRYVDSLGNPSADGLYDLALGPADSKEVCSTCVQDFNNCSGHLGHIDLPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAAIHLLVCQLKVLDVGALQAVYELERILSRFLEETSDPSAFEIQEELEEYTSKILQNNLLGSQGAHVKNVCESRSKLVAHFWKTHMAAKRCPHCKTGRSVVRKEHNSKLTITYPAMVHKKSGQKDAELPEGAPAAPGIDEAQMGKRGYLTPSSAQEHLFAIWKNEGFFLNYLFSGLDDIGPESSFNPSMFFLDFIVVPPSRYRPINRLGDQMFTNGQTVNLQAVMKDAVLIRKLLAVMAQEQKLPCEMTEITIDKENDSSGAIDRSFLSLLPGQSLTDKLYNIWIRLQSHVNIVFDSDMDKLMLEKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDATQREAVAKQLLTPSTGIPKPQGAKVVCRHVKNGDILLLNRQPTLHRPSIQAHRAHILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYTPLNLTGKAKIGSKAWVKEKPRPVPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVMRQRIIEESTQCGPRAVRAALNLPEAASCDEIQGKWQDAHLGKDQRDFNMIDMKFKEEVNHYSNEINKACMPFGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIMKSKHLHEVLSRADPQKVLRHFRAIKKWHHRHSSALLRKGAFLSFSQKIQAAVKALNLEGKTQNGRSPETQQMLQMWHELDEQSRRKYQKRAAPCPDPSLSVWRPDIHFASVSETFEKKIDDYSQEWAAQAEKSHNRSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFNTKKALRRVKSLKKQLTRVCLGEVLQKVDIQESFCMGEKQNKFRVYELRFQFLPHAYYQQEKCLRPEDILHFMETRFFKLLMEAIKKKNSKASAFRSVNTRRATQKDLDDTEDSGRNRREEERDEEEEGNIVDAEAEEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEDVQEEENIKGEGAHQTHEPDEEEGSGLEEESSQNPPRRHSRPQGAEAMERRIQAVRESHSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHNAIVYTTKGITRCLLNETINSKNEKEFVLNTEGINLPELFKYSEVLDLRRLYSNDIHAVANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQATMMAMALPPLVHFYSESDYPKCWQYEDKEAAHLAMDQKQGGKHRDPAITFKGPECQASSSPGQTCRI

Target ORF information:

RefSeq Version	XM_008762998.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polymerase (RNA) I subunit A (Polr1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762998.2

ORF Insert Sequence:

ATGCTGGCCT CCAAGCACAC GCCATGGCGG CGGCTTCAGG GCATTTCCTT CGGGATGTAT 
TCGGCCGAGG AGCTCAAGAA GTTAAGTGTT AAGTCCATTA CAAATCCTCG CTATGTGGAC 
AGCCTGGGAA ACCCTTCGGC AGATGGCCTG TATGATTTGG CTTTGGGTCC TGCAGATTCC 
AAAGAAGTAT GCTCTACCTG TGTGCAGGAC TTCAACAACT GCTCCGGACA CCTGGGCCAC 
ATTGACCTCC CGCTCACAGT GTACAACCCT CTCCTCTTCG ATAAACTCTA CCTGCTGCTG 
CGAGGCTCCT GTTTAAATTG TCATATGCTG ACTTGTCCTC GGGCTGCAAT TCACCTATTG 
GTCTGCCAGC TGAAGGTCCT AGACGTTGGA GCCCTACAAG CAGTCTATGA GCTTGAGCGG 
ATTCTCAGCA GGTTTCTGGA AGAAACTTCG GATCCTTCTG CCTTCGAAAT TCAAGAAGAG 
TTAGAAGAGT ACACAAGCAA AATTCTGCAG AATAACCTCC TGGGGTCCCA GGGTGCACAT 
GTGAAAAATG TGTGCGAGAG CAGAAGCAAA CTTGTCGCTC ACTTCTGGAA GACACACATG 
GCTGCTAAGC GCTGTCCACA CTGCAAGACG GGACGGTCAG TGGTTCGAAA GGAGCACAAT 
AGTAAGCTAA CAATAACATA TCCAGCCATG GTGCACAAGA AGTCTGGCCA GAAGGATGCA 
GAACTCCCAG AAGGAGCACC AGCAGCTCCA GGAATTGATG AGGCTCAGAT GGGGAAACGA 
GGATACTTGA CCCCAAGCAG CGCCCAAGAA CATCTTTTCG CCATTTGGAA GAATGAAGGA 
TTCTTCCTGA ATTATCTTTT TTCGGGATTG GATGATATTG GCCCAGAGTC CAGTTTCAAC 
CCCAGTATGT TCTTTCTAGA TTTCATCGTG GTACCGCCAT CCAGGTATCG TCCCATCAAT 
CGCCTGGGTG ACCAGATGTT CACTAATGGC CAGACAGTGA ATTTGCAAGC GGTCATGAAG 
GATGCAGTTT TGATCCGGAA ACTTCTGGCA GTGATGGCCC AAGAACAGAA ACTGCCCTGT 
GAGATGACAG AAATCACCAT AGACAAGGAA AATGACTCCT CGGGTGCTAT CGACCGGTCC 
TTTCTGAGTT TGCTTCCAGG ACAGTCTCTC ACAGATAAAC TTTACAATAT TTGGATTCGC 
CTCCAGAGCC ACGTCAATAT TGTGTTTGAC AGTGACATGG ACAAACTGAT GCTGGAAAAG 
TACCCTGGCA TAAGACAGAT CCTCGAGAAG AAGGAAGGCC TGTTCCGGAA GCACATGATG 
GGAAAGCGAG TGGACTACGC AGCCCGCTCG GTCATCTGCC CAGATATGTA CATCAACACC 
AATGAGATCG GGATTCCCAT GGTATTTGCC ACAAAACTGA CTTACCCCCA GCCAGTTACC 
CCCTGGAACG TGCAGGAACT GAGACAGGCA GTCATCAATG GACCCAATGT GCACCCAGGA 
GCCTCCATGG TCATCAACGA GGATGGCAGT CGCACAGCCC TGAGCGCAGT GGATGCTACA 
CAGCGGGAAG CTGTGGCCAA ACAGCTCCTG ACACCGTCCA CAGGGATCCC TAAGCCTCAG 
GGAGCAAAAG TTGTGTGCCG GCATGTGAAG AATGGGGACA TTCTCCTCCT GAACCGACAG 
CCCACCTTGC ACAGACCCTC CATCCAGGCC CACCGTGCCC ACATTCTGCC TGAGGAAAAA 
GTCCTGCGCC TTCACTATGC CAACTGCAAG GCCTACAATG CTGACTTCGA TGGAGACGAG 
ATGAATGCCC ACTTCCCCCA GAGTGAGCTG GGCCGAGCTG AAGCCTATGT CCTGGCCTGC 
ACTGACCAGC AGTATCTTGT TCCCAAGGAT GGCCAGCCCT TGGCAGGACT GATTCAGGAT 
CACATGGTGT CGGGTGCAAA CATGACCATT CGGGGATGCT TTTTCACCCG GGAACAGTAC 
ATGGAGCTGG TGTACCGAGG GCTCACAGAC AAAGTGGGAC GCGTGAAGCT CTTTCCTCCT 
GCCATCCTGA AGCCCTTCCC GCTGTGGACA GGAAAGCAGG TCGTGTCGAC CCTCCTCATA 
AACATAATCC CAGAGGACTA CACCCCCCTG AATTTAACTG GAAAGGCAAA AATCGGCAGT 
AAAGCCTGGG TGAAGGAAAA GCCTCGGCCT GTTCCTGACT TTGACCCTGA CTCCATGTGT 
GAGTCCCAGG TGATCATCAG GGAAGGGGAG CTGCTCTGCG GGGTGCTGGA CAAGGCACAC 
TATGGGAGCT CTGCCTACGG CCTGGTCCAC TGCTGCTACG AGATCTATGG GGGTGAGACC 
AGCGGCAGGG TTCTCACCTG CTTAGCCCGC CTCTTCACTG CCTACCTACA GCTCTATCGA 
GGTTTCACCT TGGGTGTGGA AGACATTTTG GTTAAGCCAA ATGCAGATGT GATGAGACAG 
CGCATCATTG AAGAATCTAC CCAGTGTGGA CCCCGGGCTG TCAGGGCTGC GTTGAACCTG 
CCAGAGGCTG CATCATGTGA TGAGATCCAG GGGAAATGGC AGGATGCCCA TCTGGGCAAA 
GACCAGAGGG ATTTTAACAT GATTGATATG AAGTTCAAGG AGGAAGTGAA CCATTACAGC 
AATGAGATTA ACAAGGCATG TATGCCTTTT GGCTTGCACA GACAATTCCC TGAAAACAAC 
CTGCAGATGA TGGTGCAGTC GGGAGCCAAA GGGTCGACCG TGAACACGAT GCAAATCTCT 
TGCCTGCTGG GCCAAATTGA GTTGGAAGGT CGGAGACCCC CACTGATGGC GTCTGGCAAG 
TCACTGCCCT GCTTTGAGCC TTACGAGTTC ACCCCAAGGG CTGGTGGCTT TGTCACTGGA 
AGGTTCCTTA CCGGTATCAG GCCACCTGAG TTCTTCTTCC ACTGCATGGC GGGGCGAGAA 
GGCCTGGTGG ATACTGCTGT CAAGACCAGC CGTTCTGGCT ATCTCCAAAG GTGTATCATC 
AAGCACCTGG AGGGTCTGGT CATCCAGTAC GATCTCACGG TCCGTGACAG TGATGGCAGT 
GTGGTACAGT TCCTGTATGG GGAGGATGGC CTGGACATCC CCAAGACACA GTTCCTACAA 
CCCAAGCAGT TCCCCTTTCT GGCCAGCAAT TATGAGGTGA TAATGAAATC CAAGCATCTT 
CATGAAGTTT TGTCTAGAGC AGATCCCCAG AAAGTGCTCC GCCACTTCAG AGCCATTAAA 
AAATGGCACC ACAGGCATTC CAGTGCCCTG CTGAGAAAAG GCGCCTTCCT GAGTTTTTCC 
CAGAAGATTC AGGCAGCTGT GAAAGCCTTG AACCTAGAGG GCAAGACCCA GAATGGGCGC 
AGCCCAGAGA CCCAGCAGAT GTTACAGATG TGGCATGAAT TAGATGAGCA GAGCCGACGG 
AAATACCAGA AGAGGGCTGC CCCGTGTCCC GACCCTAGCC TTTCTGTGTG GCGCCCAGAC 
ATCCACTTCG CATCTGTGTC TGAAACATTT GAAAAAAAGA TTGATGACTA CAGTCAGGAG 
TGGGCAGCTC AAGCAGAGAA GAGTCACAAC AGATCAGAGC TTTCTCTTGA CAGGCTGAGA 
ACCTTACTGC AGCTCAAGTG GCAGCGCTCA CTGTGTGACC CAGGCGAGGC TGTTGGCCTC 
CTGGCCGCCC AGAGCATCGG AGAGCCCTCC ACACAGATGA CACTGAACAC CTTCCACTTT 
GCAGGCAGAG GCGAGATGAA CGTCACCCTG GGCATTCCAA GATTGAGGGA GATTCTCATG 
GTGGCCAGTG CCAACATCAA AACACCCATG ATGAGCGTGC CTGTGTTCAA CACCAAGAAA 
GCACTGAGGA GAGTGAAGAG TCTGAAGAAG CAGCTCACCA GGGTGTGCCT GGGGGAGGTA 
TTGCAGAAAG TTGACATCCA GGAGTCCTTC TGTATGGGAG AGAAACAGAA TAAATTCCGG 
GTGTATGAGC TCCGGTTTCA GTTCCTGCCA CATGCATATT ACCAGCAGGA GAAGTGCCTG 
AGACCCGAGG ACATCTTGCA CTTCATGGAA ACCAGATTCT TTAAGCTACT AATGGAAGCT 
ATCAAAAAGA AGAATAGCAA AGCATCAGCC TTCAGAAGTG TGAATACCAG GAGAGCTACA 
CAGAAGGACC TCGATGACAC TGAGGACTCG GGGAGGAATC GGAGAGAGGA GGAAAGGGAT 
GAGGAAGAGG AGGGGAACAT TGTGGATGCT GAAGCTGAGG AGGGGGATGC AGATGCTTCT 
GACACCAAAC GCAAGGAGAA GCAAGAAGAA GAGGTTGACT ATGAGAGTGA GGAAGAAGGG 
GAGGAGGAAG AGGAAGAGGA TGTACAAGAA GAGGAGAACA TCAAAGGTGA AGGTGCCCAC 
CAGACCCATG AGCCAGATGA GGAAGAAGGT TCAGGCCTTG AGGAGGAATC ATCCCAGAAC 
CCTCCCCGCC GCCATTCCAG GCCTCAGGGA GCTGAGGCCA TGGAACGCCG AATCCAGGCT 
GTACGGGAGT CCCATTCGTT CATTGAGGAC TACCAGTATG ACACAGAGGA GAGCCTGTGG 
TGCCAGGTGA CAGTGAAGCT CCCATTGATG AAGATTAATT TTGACATGAG CTCCTTGGTT 
GTATCCTTGG CCCACAATGC CATTGTCTAT ACGACTAAGG GCATCACTCG GTGTCTCCTG 
AATGAAACGA TCAACAGTAA GAATGAGAAG GAGTTTGTCC TGAACACGGA GGGAATCAAC 
CTCCCCGAGC TGTTCAAGTA CTCCGAGGTG CTGGACCTGC GCCGCCTGTA CTCCAACGAC 
ATCCATGCTG TGGCCAATAC GTACGGCATC GAGGCTGCGC TGCGGGTGAT AGAGAAGGAG 
ATTAAGGATG TGTTTGCTGT GTACGGCATT GCGGTTGATC CTCGCCATCT CTCCCTGGTT 
GCTGATTATA TGTGTTTTGA GGGTGTTTAT AAGCCACTCA ATCGCTTTGG GATCCAGTCA 
AGCTCCTCCC CTTTGCAGCA GATGACGTTT GAAACCAGCT TCCAGTTTCT GAAACAGGCC 
ACCATGATGG CCATGGCCTT GCCACCGTTG GTTCACTTCT ACAGTGAGTC AGACTATCCC 
AAGTGCTGGC AATATGAGGA TAAAGAGGCT GCTCACCTTG CAATGGACCA GAAGCAGGGA 
GGAAAGCACC GAGATCCAGC TATAACTTTC AAAGGCCCTG AGTGCCAGGC AAGCAGCTCA 
CCAGGGCAGA CCTGCAGAAT CTGA