eStain™ L1 Protein Staining System

Highlights

Automated fast staining

Consistent results for Coomassie Blue Staining

Membrane staining

Easy to use with clean and convenient operation

Customer Testimonials

"We've incorporated eStain, the automatic gel staining system into our laboratory operations, which has notably reduced the time required for downstream protein detection. Overall, the suite of automated solutions provided by GenScript has streamlined our research endeavors considerably and made our research easy."

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GenScript eStain User

Experienced scientist with expertise on
neglected tropical diseases (NTDs)

"The eStain Automated Protein Staining Device reduced staining time from 2-3 hours to just 10 minutes, with consistent quality. Our team of scientists relies on eStain for all our protein staining work. I would say no one should be doing manual staining when there is a readily available automated solution in the market, such as the eStain." Read More >>

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Andrew Lees

Fina BioSolutions LLC

eStain Videos

• Video

  Introductory Video

  Detailed Set Up and Operation Video

Why choose eStain™ L1

• Shortened workflow increases your productivity

  The eStain Protein Staining System combines three steps of traditional Coomassie blue staining method into one single step to greatly reduce the time required for protein staining (Table 1).

  	Wash or Rinse Step	Stain Step	Destain or Rinse Step	Time saved with eStain L1
  eStain™	0 min	Electric Staining <10 min	0 min
  Staining reagent A	Rinse 15 min	Stain 1-3 hr	Wash 1-3 hr	2 hr , 15 min-6 hr, 15 min
  Staining reagent B	Wash 15 min	Stain 1 hr	Rinse 30 min	1 hr , 45 min
  Gelcode Blue Safe Protein Stain (Basic Protocol)	Wash 15 min	Stain 15 min-1 hr	Destain 1-2 hr	1 hr , 30 min - 3 hr , 15 min

• Excellent sensitivity and repeatability with Coomassie Blue
  • Gel: ExpressPlus™ PAGE Gel, 4-12%, 10 wells (GenScript M41210)
  • Program: Stain
  • Staining solution: Coomasie Blue
  • Time: 9 min 30 s

  
• Compatibility for membrane staining with Ponceau S solution
  • Gel: SurePAGE™ Gel, Bis-Tris, 4-12% (GenScript, M00653)
  • Membrane: GenScript nitrocellulose, PVDF membranes
  • Samples: Pre-stained protein standard (GenScript, M006245); unstained PAGE-Master protein standard (GenScript, M00516)
  • Program: Stain
  • Staining Solution: Ponceau S solution
  • Time: 9 min 30 s

  

  

Quick Procedures

• eStain Protein Staining device quick guide

  Note: For complete instructions and advanced programming, please refer to the Manual

  Operational Instructions
  Step 1. Add distilled water into the tray provided. When gel electrophoresis is complete, carefully remove the gel from the gel cassette and briefly rinse the gel with distilled water for 1 min
  Step 2. Open the gel holder and place it on the table with the fabric side down. Carefully place the rinsed gel on the fabric side of the gel holder. Ensure that the upper  part of the gel (with comb) point to the axis of the gel holder and is as close to the axis as possible
  Step 3. Pre-wet a piece of filter paper (provided) with distilled water and place it on top of the gel
  Step 4. Close the gel holder
  Step 5. Choose a Channel and insert the gel holder into the channel. Note：Please ensure that the fabric side of the gel holder is facing the user before inserting it into the Channel
  Step 6. Press the corresponding channel to start the program. The channel timer starts to countdown
  Step 7. The instrument beeps as the program countdown to 0
  Step 8. The channel stops flashing and the screen returns to normal. Take out the gel holder and the stained gel. The staining process is complete.

Product List

eStain™ L1 Resources

eStain User Manual
estain L1 Quick Reference Card
eStain Flyer
Ponceau Staining Application Note

Technical Resources

Product Documents

Learn More

Product FAQ Product Publications Product News Other Technical Support