Gli3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gli3
Entrez Gene ID	14634
Full Name	GLI-Kruppel family member GLI3
Synonyms	AI854843,AU023367,Bph,GLI3-190,GLI3FL,Pdn,Xt,add
General protein information	Preferred Names GLI-Kruppel family member GLI3 Names transcriptional activator GLI3 GLI3 form of 190 kDa GLI3 full length protein anterior digit pattern deformity brachyphalangy extra toes polydactyly Nagoya zinc finger protein GLI3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A1\|13 5.43 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008130.2	NP_032156.2	transcriptional activator GLI3
XM_017315389.1	XP_017170878.1	transcriptional activator GLI3 isoform X2
XM_006516552.3	XP_006516615.1	transcriptional activator GLI3 isoform X1
NM_008130.3	NP_032156.2	transcriptional activator GLI3

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
Macaca mulatta (Rhesus monkey)	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Gene Ontology
Bibliography

Related articles in PubMed

Primary cilia in murine palatal rugae development.
Nakaniwa M, Kawasaki M, Kawasaki K, Yamada A, Meguro F, Takeyasu M, Ohazama A
Gene expression patterns : GEP34119062(2019 12)

Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals.
Yung T, Poon F, Liang M, Coquenlorge S, McGaugh EC, Hui CC, Wilson MD, Nostro MC, Kim TH
Nature communications10(1)4647(2019 10)

Derepression of sonic hedgehog signaling upon Gpr161 deletion unravels forebrain and ventricular abnormalities.
Shimada IS, Somatilaka BN, Hwang SH, Anderson AG, Shelton JM, Rajaram V, Konopka G, Mukhopadhyay S
Developmental biology450(1)47-62(2019 06)

GLI3 repressor but not GLI3 activator is essential for mouse eye patterning and morphogenesis.
Wiegering A, Petzsch P, Köhrer K, Rüther U, Gerhardt C
Developmental biology450(2)141-154(2019 06)

Secreted metalloproteases ADAMTS9 and ADAMTS20 have a non-canonical role in ciliary vesicle growth during ciliogenesis.
Nandadasa S, Kraft CM, Wang LW, O'Donnell A, Patel R, Gee HY, Grobe K, Cox TC, Hildebrandt F, Apte SS
Nature communications10(1)953(2019 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gli3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu78425	XM_017315389.1 Latest version!	Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu21819	NM_008130.3 Latest version!	Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu48321	XM_006516552.3 Latest version!	Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OMu21819	NM_008130.2	Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gli3 ( XM_017315389.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170878.1 Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X2, mRNA.

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CloneID	OMu78425
Clone ID Related Accession (Same CDS sequence)	XM_017315389.1
Accession Version	XM_017315389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGTT CTCACTTCCT AAGGACAGTG GGTAATAAGA CATCTCAGCC AAGCCCTGGT 
GGTCAGTCTT CATGCAGCAG CCAACAGTCC CCCATCAGCA ACTATTCCAA CAGTGGGCTC 
GAGCTTCCTC TGACTGATGG AGGTAGTGTA GCAGACCTCA GTGCCATCGA TGAAACCCCA 
ATCATGGACT CGACCATTTC CACGGCAACC ACAGCCCTTG CTTTGCAAGC CAGGAGAAAC 
CCGGCAGGGA CCAAATGGAT GGAGCACATC AAACTTGAAA GGCTAAAACA AGTGAATGGA 
ATGTTTCCAA GACTGAACCC TATTCTACCC TCCAAAGCCC CTGCGGTATC TCCTCTCATA 
GGAAATGGCA CACAGTCCAA TAACAACTAT AGCTCGGGTG GGCCCGGGAC CCTCCTCCCG 
AGCAGAAGTG ACCTGTCAGG TGTAGACTTC ACAGTGCTGA ACACACTCAA CAGGAGAGAC 
AGCAATACCA GCACTATCAG CTCTGCCTAC CTGAGCAGCC GCAGATCCTC GGGCATCTCC 
CCCTGCTTTT CCAGCCGCAG GTCCAGCGAG GCGTCCCAGG CTGAAGGGCG ACCCCAGAAT 
GTGAGTGTGG CTGACTCCTA CGATCCCATC TCCACAGATG CTTCAAGGAG GTCCAGCGAG 
GCCAGCCAGG GTGACGGGCT GCCCAGTCTG CTCAGCCTCA CACCCGTGCA GCAGTACCGC 
CTTAAGGCCA AGTATGCAGC TGCCACAGGT GGCCCACCAC CCACACCTCT GCCCCACATG 
GAGAGGCTGA GCCTGAAGAC CAAGATGGCC TTGCTAGGGG AAGGGAGGGA CTCTGGGGTG 
ACCCTGCCTC CAGTCCATCC TCCTCGAAGA TGCAGTGATG GGGGAGGTCA CACATACAGA 
GGGCGTCACC TGATGCCTCA TGATGCACTA GCGAACAGTG TGAGGAGAGC CAGCGACCCT 
GTGAGGACAG TCTCGGAGAA CATGTCACTT GCCAGGGTTC AACGCTTCAG CAGCCTCAAT 
AGCTTTAATC CTCCAAATTT GCCTCCATCG GTGGAAAAGC GTAGCTTAGT GCTGCAAAAT 
TATACCCGGC AGGAGAGCAG CCAACCCCGG TACTTCCAGG CATCCCCTTG CCCTCCCAGC 
ATCACAGAGA ATGTTGCCCT GGAGGCTCTG ACCATGGATG CTGATGCTAA CTTGAATGAT 
GAAGACCTCC TGCCAGATGA TGTGGTACAG TATTTAAATT CCCAGAACCA AACAGGGTAT 
GGGCAGCAGT TGCAGAGTGG CATCTCTGAA GACAGCAAAG TAGCCCATGA GCCAGAGGAC 
TTGGACTTAG CAGGGCTGCC AGACAGTCAT GTTGGCCAGG AGTACCCAGC TCTGGAGCAG 
CCCTGCTCTG AGGGCAGTAA AACTGATTTG CCCATCCAGT GGAACGAGGT CAGTTCTGGA 
ACTTCTGATC TGTCATCCTC CAAGCTGAAG TGTGGTCAGC AGCGCCCTAG CGCACAGCAG 
CCTCGGGGTT TTGGGCTATA CAACAATATG GTGGTACACC CACATAATCT GTGGAAGGTT 
GGCACTGGCC CAGCTGGGGG ATATCAGACC CTCGGGGAGA ATAGCAGTAC CTACAATGGC 
CCAGAGCACT TTGCAATCCA CAGTGGGGAT GGACTTGGCA CCAATGGAAA TACTTTCCAT 
GAACAGCCCT TTAAGACCCA GCAGTATGGG AGCCAGCTCA ACAGGCAGCC ACTGACTTCC 
AGTGCTCTAG ATCATGCCTG TGGTACAGGG ATTCAAGGGT CCAAGCTAAA AGGCAACAGC 
TTGCAAGAGA ATGGGGGTTT GCTAGATTTC AGCCTGTCCG TGGCACCAAA TGAGTTAGCT 
GGCAACACAG TGAATGGCAT GCAAACCCAA GATCAAATGG GACAGGGATA CATTGCCCAT 
CAGCTACTCA GTGGCAGCAT GCAACACCAG GGGCCCAGCC GCCCTGGTCA ACAGGTACTA 
GGGCAGGTTG GTGCTACCTC ACATATCAAC ATCTATCAAG GGACAGAGAG CTGCCTACCA 
GGGACTCAGG ACAACAGCAG CCAGCCATCA AGCATGGCAG CTATCAGGGG CTACCAGCCC 
TGTGCCAGCT ATGGGGGTAA CAGGCGTCAG GCAATGCCAA GGGGCAACCT CACTCTGCAA 
CAAGGACAGC TCAGTGACAT GAGTCAGAGC AGCAGGGTGA ACAGCATCAA AATGGAGGCA 
CAAGGTCAGT CCCAGCAGCT CTGCTCTACC GTGCAGAATT ATTCCGGTCA GTTCTATGAC 
CAAACCATGG GCTTCAGTCA GCAAGACAGG AAAGCTGGCT CGTTCTCCCT CTCAGATGCC 
AACTGCCTGC TCCAAGGGAA TGGCACTGAA AACTCTGAGT TACTCTCCCC AGGTGCTAAC 
CAGGTAACAA GCACAGTTGA CAGCTTTGAG AGTCATGACC TAGAAGGTGT GCAGATTGAT 
TTTGATGCCA TCATAGATGA TGGGGACCAT ACCAGCCTAA TGTCAGGGGC CTTGAGCCCA 
AGTATTATTC AGAACCTTTC CCACAGCTCC TCCCGTCTCA CCACTCCGCG GGCATCCCTC 
CCATTCCCAT CCCTATCCAT GGGCACCACC AACATGGCTA TCGGGGATAT GAGTTCTTTG 
CTGACCTCCC TTGCAGAAGA AAGCAAGTTC CTTGCAGTTA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170878.1
CDS	49..2736
Translation	MSCSHFLRTVGNKTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSVADLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHIKLERLKQVNGMFPRLNPILPSKAPAVSPLIGNGTQSNNNYSSGGPGTLLPSRSDLSGVDFTVLNTLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPVQQYRLKAKYAAATGGPPPTPLPHMERLSLKTKMALLGEGRDSGVTLPPVHPPRRCSDGGGHTYRGRHLMPHDALANSVRRASDPVRTVSENMSLARVQRFSSLNSFNPPNLPPSVEKRSLVLQNYTRQESSQPRYFQASPCPPSITENVALEALTMDADANLNDEDLLPDDVVQYLNSQNQTGYGQQLQSGISEDSKVAHEPEDLDLAGLPDSHVGQEYPALEQPCSEGSKTDLPIQWNEVSSGTSDLSSSKLKCGQQRPSAQQPRGFGLYNNMVVHPHNLWKVGTGPAGGYQTLGENSSTYNGPEHFAIHSGDGLGTNGNTFHEQPFKTQQYGSQLNRQPLTSSALDHACGTGIQGSKLKGNSLQENGGLLDFSLSVAPNELAGNTVNGMQTQDQMGQGYIAHQLLSGSMQHQGPSRPGQQVLGQVGATSHINIYQGTESCLPGTQDNSSQPSSMAAIRGYQPCASYGGNRRQAMPRGNLTLQQGQLSDMSQSSRVNSIKMEAQGQSQQLCSTVQNYSGQFYDQTMGFSQQDRKAGSFSLSDANCLLQGNGTENSELLSPGANQVTSTVDSFESHDLEGVQIDFDAIIDDGDHTSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPSLSMGTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_017315389.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315389.1

ORF Insert Sequence:

ATGTCTTGTT CTCACTTCCT AAGGACAGTG GGTAATAAGA CATCTCAGCC AAGCCCTGGT 
GGTCAGTCTT CATGCAGCAG CCAACAGTCC CCCATCAGCA ACTATTCCAA CAGTGGGCTC 
GAGCTTCCTC TGACTGATGG AGGTAGTGTA GCAGACCTCA GTGCCATCGA TGAAACCCCA 
ATCATGGACT CGACCATTTC CACGGCAACC ACAGCCCTTG CTTTGCAAGC CAGGAGAAAC 
CCGGCAGGGA CCAAATGGAT GGAGCACATC AAACTTGAAA GGCTAAAACA AGTGAATGGA 
ATGTTTCCAA GACTGAACCC TATTCTACCC TCCAAAGCCC CTGCGGTATC TCCTCTCATA 
GGAAATGGCA CACAGTCCAA TAACAACTAT AGCTCGGGTG GGCCCGGGAC CCTCCTCCCG 
AGCAGAAGTG ACCTGTCAGG TGTAGACTTC ACAGTGCTGA ACACACTCAA CAGGAGAGAC 
AGCAATACCA GCACTATCAG CTCTGCCTAC CTGAGCAGCC GCAGATCCTC GGGCATCTCC 
CCCTGCTTTT CCAGCCGCAG GTCCAGCGAG GCGTCCCAGG CTGAAGGGCG ACCCCAGAAT 
GTGAGTGTGG CTGACTCCTA CGATCCCATC TCCACAGATG CTTCAAGGAG GTCCAGCGAG 
GCCAGCCAGG GTGACGGGCT GCCCAGTCTG CTCAGCCTCA CACCCGTGCA GCAGTACCGC 
CTTAAGGCCA AGTATGCAGC TGCCACAGGT GGCCCACCAC CCACACCTCT GCCCCACATG 
GAGAGGCTGA GCCTGAAGAC CAAGATGGCC TTGCTAGGGG AAGGGAGGGA CTCTGGGGTG 
ACCCTGCCTC CAGTCCATCC TCCTCGAAGA TGCAGTGATG GGGGAGGTCA CACATACAGA 
GGGCGTCACC TGATGCCTCA TGATGCACTA GCGAACAGTG TGAGGAGAGC CAGCGACCCT 
GTGAGGACAG TCTCGGAGAA CATGTCACTT GCCAGGGTTC AACGCTTCAG CAGCCTCAAT 
AGCTTTAATC CTCCAAATTT GCCTCCATCG GTGGAAAAGC GTAGCTTAGT GCTGCAAAAT 
TATACCCGGC AGGAGAGCAG CCAACCCCGG TACTTCCAGG CATCCCCTTG CCCTCCCAGC 
ATCACAGAGA ATGTTGCCCT GGAGGCTCTG ACCATGGATG CTGATGCTAA CTTGAATGAT 
GAAGACCTCC TGCCAGATGA TGTGGTACAG TATTTAAATT CCCAGAACCA AACAGGGTAT 
GGGCAGCAGT TGCAGAGTGG CATCTCTGAA GACAGCAAAG TAGCCCATGA GCCAGAGGAC 
TTGGACTTAG CAGGGCTGCC AGACAGTCAT GTTGGCCAGG AGTACCCAGC TCTGGAGCAG 
CCCTGCTCTG AGGGCAGTAA AACTGATTTG CCCATCCAGT GGAACGAGGT CAGTTCTGGA 
ACTTCTGATC TGTCATCCTC CAAGCTGAAG TGTGGTCAGC AGCGCCCTAG CGCACAGCAG 
CCTCGGGGTT TTGGGCTATA CAACAATATG GTGGTACACC CACATAATCT GTGGAAGGTT 
GGCACTGGCC CAGCTGGGGG ATATCAGACC CTCGGGGAGA ATAGCAGTAC CTACAATGGC 
CCAGAGCACT TTGCAATCCA CAGTGGGGAT GGACTTGGCA CCAATGGAAA TACTTTCCAT 
GAACAGCCCT TTAAGACCCA GCAGTATGGG AGCCAGCTCA ACAGGCAGCC ACTGACTTCC 
AGTGCTCTAG ATCATGCCTG TGGTACAGGG ATTCAAGGGT CCAAGCTAAA AGGCAACAGC 
TTGCAAGAGA ATGGGGGTTT GCTAGATTTC AGCCTGTCCG TGGCACCAAA TGAGTTAGCT 
GGCAACACAG TGAATGGCAT GCAAACCCAA GATCAAATGG GACAGGGATA CATTGCCCAT 
CAGCTACTCA GTGGCAGCAT GCAACACCAG GGGCCCAGCC GCCCTGGTCA ACAGGTACTA 
GGGCAGGTTG GTGCTACCTC ACATATCAAC ATCTATCAAG GGACAGAGAG CTGCCTACCA 
GGGACTCAGG ACAACAGCAG CCAGCCATCA AGCATGGCAG CTATCAGGGG CTACCAGCCC 
TGTGCCAGCT ATGGGGGTAA CAGGCGTCAG GCAATGCCAA GGGGCAACCT CACTCTGCAA 
CAAGGACAGC TCAGTGACAT GAGTCAGAGC AGCAGGGTGA ACAGCATCAA AATGGAGGCA 
CAAGGTCAGT CCCAGCAGCT CTGCTCTACC GTGCAGAATT ATTCCGGTCA GTTCTATGAC 
CAAACCATGG GCTTCAGTCA GCAAGACAGG AAAGCTGGCT CGTTCTCCCT CTCAGATGCC 
AACTGCCTGC TCCAAGGGAA TGGCACTGAA AACTCTGAGT TACTCTCCCC AGGTGCTAAC 
CAGGTAACAA GCACAGTTGA CAGCTTTGAG AGTCATGACC TAGAAGGTGT GCAGATTGAT 
TTTGATGCCA TCATAGATGA TGGGGACCAT ACCAGCCTAA TGTCAGGGGC CTTGAGCCCA 
AGTATTATTC AGAACCTTTC CCACAGCTCC TCCCGTCTCA CCACTCCGCG GGCATCCCTC 
CCATTCCCAT CCCTATCCAT GGGCACCACC AACATGGCTA TCGGGGATAT GAGTTCTTTG 
CTGACCTCCC TTGCAGAAGA AAGCAAGTTC CTTGCAGTTA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli3 ( NM_008130.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032156.2 Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA.

Price & Availability↑

CloneID	OMu21819
Clone ID Related Accession (Same CDS sequence)	NM_008130.2 , NM_008130.3
Accession Version	NM_008130.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514563200000
Organism	Mus musculus(house mouse)
Product	transcriptional activator GLI3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158261.1, X95255.1, CA752350.1 and AC173210.3. On Jan 5, 2007 this sequence version replaced NM_008130.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X95255.1, BC141135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG 
CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT 
GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC 
TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT 
GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT 
AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT 
ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA 
GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG 
ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT 
GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC 
AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA 
GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT 
GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT 
CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC 
ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG 
AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGAGCAAGCC CACAAGCGAG 
TCTGCAGTGA GCAGCACTGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCTGAT 
GAAGACCTCC CCAGCCCAGG GTCACGGGGC CAGCAGGAAC AGCCGGAAGG AACAACCCTA 
GTCAAGGAGG AAGCGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTACGAGACA 
AACTGCCACT GGGAAGGCTG CACCAGAGAG TTCGACACCC AAGATCAGCT TGTGCATCAT 
ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTCG TGTGCCGCTG GCTTGATTGT 
TCACGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTGCATAT GAGAAGACAC 
ACTGGGGAGA AGCCTCACAA ATGTACATTT GAAGGTTGCA CAAAAGCCTA CTCAAGACTC 
GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATACGT CTGTGAGCAT 
GAGGGCTGCA ACAAGGCTTT CTCTAATGCT TCAGATCGGG CCAAGCACCA AAACAGAACA 
CATTCCAATG AGAAACCGTA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC 
CCCAGCTCTC TCCGGAAACA CGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG 
AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CCCCTGGCCG GCCAACTCAG GGAGCATTTG GTGAGCAGAA GGAGCTGAGC 
AACACTACCT CAAAGCGGGA AGAGTGCCTC CAGGTGAAGA CTGTCAAGGC TGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TGTAGCAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CATCAAACTT 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA 
GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CCAATAACAA CTATAGCTCG 
GGTGGGCCCG GGACCCTCCT CCCGAGCAGA AGTGACCTGT CAGGTGTAGA CTTCACAGTG 
CTGAACACAC TCAACAGGAG AGACAGCAAT ACCAGCACTA TCAGCTCTGC CTACCTGAGC 
AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG CGAGGCGTCC 
CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCTGACT CCTACGATCC CATCTCCACA 
GATGCTTCAA GGAGGTCCAG CGAGGCCAGC CAGGGTGACG GGCTGCCCAG TCTGCTCAGC 
CTCACACCCG TGCAGCAGTA CCGCCTTAAG GCCAAGTATG CAGCTGCCAC AGGTGGCCCA 
CCACCCACAC CTCTGCCCCA CATGGAGAGG CTGAGCCTGA AGACCAAGAT GGCCTTGCTA 
GGGGAAGGGA GGGACTCTGG GGTGACCCTG CCTCCAGTCC ATCCTCCTCG AAGATGCAGT 
GATGGGGGAG GTCACACATA CAGAGGGCGT CACCTGATGC CTCATGATGC ACTAGCGAAC 
AGTGTGAGGA GAGCCAGCGA CCCTGTGAGG ACAGTCTCGG AGAACATGTC ACTTGCCAGG 
GTTCAACGCT TCAGCAGCCT CAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCGGTGGAA 
AAGCGTAGCT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCAGCCAACC CCGGTACTTC 
CAGGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC TCTGACCATG 
GATGCTGATG CTAACTTGAA TGATGAAGAC CTCCTGCCAG ATGATGTGGT ACAGTATTTA 
AATTCCCAGA ACCAAACAGG GTATGGGCAG CAGTTGCAGA GTGGCATCTC TGAAGACAGC 
AAAGTAGCCC ATGAGCCAGA GGACTTGGAC TTAGCAGGGC TGCCAGACAG TCATGTTGGC 
CAGGAGTACC CAGCTCTGGA GCAGCCCTGC TCTGAGGGCA GTAAAACTGA TTTGCCCATC 
CAGTGGAACG AGGTCAGTTC TGGAACTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT 
CAGCAGCGCC CTAGCGCACA GCAGCCTCGG GGTTTTGGGC TATACAACAA TATGGTGGTA 
CACCCACATA ATCTGTGGAA GGTTGGCACT GGCCCAGCTG GGGGATATCA GACCCTCGGG 
GAGAATAGCA GTACCTACAA TGGCCCAGAG CACTTTGCAA TCCACAGTGG GGATGGACTT 
GGCACCAATG GAAATACTTT CCATGAACAG CCCTTTAAGA CCCAGCAGTA TGGGAGCCAG 
CTCAACAGGC AGCCACTGAC TTCCAGTGCT CTAGATCATG CCTGTGGTAC AGGGATTCAA 
GGGTCCAAGC TAAAAGGCAA CAGCTTGCAA GAGAATGGGG GTTTGCTAGA TTTCAGCCTG 
TCCGTGGCAC CAAATGAGTT AGCTGGCAAC ACAGTGAATG GCATGCAAAC CCAAGATCAA 
ATGGGACAGG GATACATTGC CCATCAGCTA CTCAGTGGCA GCATGCAACA CCAGGGGCCC 
AGCCGCCCTG GTCAACAGGT ACTAGGGCAG GTTGGTGCTA CCTCACATAT CAACATCTAT 
CAAGGGACAG AGAGCTGCCT ACCAGGGACT CAGGACAACA GCAGCCAGCC ATCAAGCATG 
GCAGCTATCA GGGGCTACCA GCCCTGTGCC AGCTATGGGG GTAACAGGCG TCAGGCAATG 
CCAAGGGGCA ACCTCACTCT GCAACAAGGA CAGCTCAGTG ACATGAGTCA GAGCAGCAGG 
GTGAACAGCA TCAAAATGGA GGCACAAGGT CAGTCCCAGC AGCTCTGCTC TACCGTGCAG 
AATTATTCCG GTCAGTTCTA TGACCAAACC ATGGGCTTCA GTCAGCAAGA CAGGAAAGCT 
GGCTCGTTCT CCCTCTCAGA TGCCAACTGC CTGCTCCAAG GGAATGGCAC TGAAAACTCT 
GAGTTACTCT CCCCAGGTGC TAACCAGGTA ACAAGCACAG TTGACAGCTT TGAGAGTCAT 
GACCTAGAAG GTGTGCAGAT TGATTTTGAT GCCATCATAG ATGATGGGGA CCATACCAGC 
CTAATGTCAG GGGCCTTGAG CCCAAGTATT ATTCAGAACC TTTCCCACAG CTCCTCCCGT 
CTCACCACTC CGCGGGCATC CCTCCCATTC CCATCCCTAT CCATGGGCAC CACCAACATG 
GCTATCGGGG ATATGAGTTC TTTGCTGACC TCCCTTGCAG AAGAAAGCAA GTTCCTTGCA 
GTTATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032156.2
CDS	432..5183
Translation	MEAQAHSSTATERKKAENSIGKCPTRTDVSEKAVASSTTSNEDESPGQIYHRERRNAITMQPQSVQGLNKISEEPSTSSDERASLIKKEIHGSLPHLAEPSLPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADILPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQSKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGSRGQQEQPEGTTLVKEEADKDESKQEPEVIYETNCHWEGCTREFDTQDQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDMHPRPPPPRDSGSHSQSRSPGRPTQGAFGEQKELSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSVADLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHIKLERLKQVNGMFPRLNPILPSKAPAVSPLIGNGTQSNNNYSSGGPGTLLPSRSDLSGVDFTVLNTLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPVQQYRLKAKYAAATGGPPPTPLPHMERLSLKTKMALLGEGRDSGVTLPPVHPPRRCSDGGGHTYRGRHLMPHDALANSVRRASDPVRTVSENMSLARVQRFSSLNSFNPPNLPPSVEKRSLVLQNYTRQESSQPRYFQASPCPPSITENVALEALTMDADANLNDEDLLPDDVVQYLNSQNQTGYGQQLQSGISEDSKVAHEPEDLDLAGLPDSHVGQEYPALEQPCSEGSKTDLPIQWNEVSSGTSDLSSSKLKCGQQRPSAQQPRGFGLYNNMVVHPHNLWKVGTGPAGGYQTLGENSSTYNGPEHFAIHSGDGLGTNGNTFHEQPFKTQQYGSQLNRQPLTSSALDHACGTGIQGSKLKGNSLQENGGLLDFSLSVAPNELAGNTVNGMQTQDQMGQGYIAHQLLSGSMQHQGPSRPGQQVLGQVGATSHINIYQGTESCLPGTQDNSSQPSSMAAIRGYQPCASYGGNRRQAMPRGNLTLQQGQLSDMSQSSRVNSIKMEAQGQSQQLCSTVQNYSGQFYDQTMGFSQQDRKAGSFSLSDANCLLQGNGTENSELLSPGANQVTSTVDSFESHDLEGVQIDFDAIIDDGDHTSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPSLSMGTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	NM_008130.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008130.2

ORF Insert Sequence:

ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG 
CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT 
GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC 
TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT 
GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT 
AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT 
ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA 
GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG 
ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT 
GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC 
AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA 
GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT 
GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT 
CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC 
ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG 
AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGAGCAAGCC CACAAGCGAG 
TCTGCAGTGA GCAGCACTGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCTGAT 
GAAGACCTCC CCAGCCCAGG GTCACGGGGC CAGCAGGAAC AGCCGGAAGG AACAACCCTA 
GTCAAGGAGG AAGCGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTACGAGACA 
AACTGCCACT GGGAAGGCTG CACCAGAGAG TTCGACACCC AAGATCAGCT TGTGCATCAT 
ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTCG TGTGCCGCTG GCTTGATTGT 
TCACGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTGCATAT GAGAAGACAC 
ACTGGGGAGA AGCCTCACAA ATGTACATTT GAAGGTTGCA CAAAAGCCTA CTCAAGACTC 
GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATACGT CTGTGAGCAT 
GAGGGCTGCA ACAAGGCTTT CTCTAATGCT TCAGATCGGG CCAAGCACCA AAACAGAACA 
CATTCCAATG AGAAACCGTA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC 
CCCAGCTCTC TCCGGAAACA CGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG 
AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CCCCTGGCCG GCCAACTCAG GGAGCATTTG GTGAGCAGAA GGAGCTGAGC 
AACACTACCT CAAAGCGGGA AGAGTGCCTC CAGGTGAAGA CTGTCAAGGC TGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TGTAGCAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CATCAAACTT 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA 
GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CCAATAACAA CTATAGCTCG 
GGTGGGCCCG GGACCCTCCT CCCGAGCAGA AGTGACCTGT CAGGTGTAGA CTTCACAGTG 
CTGAACACAC TCAACAGGAG AGACAGCAAT ACCAGCACTA TCAGCTCTGC CTACCTGAGC 
AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG CGAGGCGTCC 
CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCTGACT CCTACGATCC CATCTCCACA 
GATGCTTCAA GGAGGTCCAG CGAGGCCAGC CAGGGTGACG GGCTGCCCAG TCTGCTCAGC 
CTCACACCCG TGCAGCAGTA CCGCCTTAAG GCCAAGTATG CAGCTGCCAC AGGTGGCCCA 
CCACCCACAC CTCTGCCCCA CATGGAGAGG CTGAGCCTGA AGACCAAGAT GGCCTTGCTA 
GGGGAAGGGA GGGACTCTGG GGTGACCCTG CCTCCAGTCC ATCCTCCTCG AAGATGCAGT 
GATGGGGGAG GTCACACATA CAGAGGGCGT CACCTGATGC CTCATGATGC ACTAGCGAAC 
AGTGTGAGGA GAGCCAGCGA CCCTGTGAGG ACAGTCTCGG AGAACATGTC ACTTGCCAGG 
GTTCAACGCT TCAGCAGCCT CAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCGGTGGAA 
AAGCGTAGCT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCAGCCAACC CCGGTACTTC 
CAGGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC TCTGACCATG 
GATGCTGATG CTAACTTGAA TGATGAAGAC CTCCTGCCAG ATGATGTGGT ACAGTATTTA 
AATTCCCAGA ACCAAACAGG GTATGGGCAG CAGTTGCAGA GTGGCATCTC TGAAGACAGC 
AAAGTAGCCC ATGAGCCAGA GGACTTGGAC TTAGCAGGGC TGCCAGACAG TCATGTTGGC 
CAGGAGTACC CAGCTCTGGA GCAGCCCTGC TCTGAGGGCA GTAAAACTGA TTTGCCCATC 
CAGTGGAACG AGGTCAGTTC TGGAACTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT 
CAGCAGCGCC CTAGCGCACA GCAGCCTCGG GGTTTTGGGC TATACAACAA TATGGTGGTA 
CACCCACATA ATCTGTGGAA GGTTGGCACT GGCCCAGCTG GGGGATATCA GACCCTCGGG 
GAGAATAGCA GTACCTACAA TGGCCCAGAG CACTTTGCAA TCCACAGTGG GGATGGACTT 
GGCACCAATG GAAATACTTT CCATGAACAG CCCTTTAAGA CCCAGCAGTA TGGGAGCCAG 
CTCAACAGGC AGCCACTGAC TTCCAGTGCT CTAGATCATG CCTGTGGTAC AGGGATTCAA 
GGGTCCAAGC TAAAAGGCAA CAGCTTGCAA GAGAATGGGG GTTTGCTAGA TTTCAGCCTG 
TCCGTGGCAC CAAATGAGTT AGCTGGCAAC ACAGTGAATG GCATGCAAAC CCAAGATCAA 
ATGGGACAGG GATACATTGC CCATCAGCTA CTCAGTGGCA GCATGCAACA CCAGGGGCCC 
AGCCGCCCTG GTCAACAGGT ACTAGGGCAG GTTGGTGCTA CCTCACATAT CAACATCTAT 
CAAGGGACAG AGAGCTGCCT ACCAGGGACT CAGGACAACA GCAGCCAGCC ATCAAGCATG 
GCAGCTATCA GGGGCTACCA GCCCTGTGCC AGCTATGGGG GTAACAGGCG TCAGGCAATG 
CCAAGGGGCA ACCTCACTCT GCAACAAGGA CAGCTCAGTG ACATGAGTCA GAGCAGCAGG 
GTGAACAGCA TCAAAATGGA GGCACAAGGT CAGTCCCAGC AGCTCTGCTC TACCGTGCAG 
AATTATTCCG GTCAGTTCTA TGACCAAACC ATGGGCTTCA GTCAGCAAGA CAGGAAAGCT 
GGCTCGTTCT CCCTCTCAGA TGCCAACTGC CTGCTCCAAG GGAATGGCAC TGAAAACTCT 
GAGTTACTCT CCCCAGGTGC TAACCAGGTA ACAAGCACAG TTGACAGCTT TGAGAGTCAT 
GACCTAGAAG GTGTGCAGAT TGATTTTGAT GCCATCATAG ATGATGGGGA CCATACCAGC 
CTAATGTCAG GGGCCTTGAG CCCAAGTATT ATTCAGAACC TTTCCCACAG CTCCTCCCGT 
CTCACCACTC CGCGGGCATC CCTCCCATTC CCATCCCTAT CCATGGGCAC CACCAACATG 
GCTATCGGGG ATATGAGTTC TTTGCTGACC TCCCTTGCAG AAGAAAGCAA GTTCCTTGCA 
GTTATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli3 ( NM_008130.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032156.2 Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA.

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CloneID	OMu21819
Clone ID Related Accession (Same CDS sequence)	NM_008130.2 , NM_008130.3
Accession Version	NM_008130.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Mus musculus(house mouse)
Product	transcriptional activator GLI3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163610.4, AC173115.2 and AC173210.3. On Aug 14, 2018 this sequence version replaced NM_008130.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC141135.1, X95255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG 
CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT 
GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC 
TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT 
GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT 
AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT 
ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA 
GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG 
ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT 
GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC 
AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA 
GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT 
GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT 
CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC 
ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG 
AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGAGCAAGCC CACAAGCGAG 
TCTGCAGTGA GCAGCACTGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCTGAT 
GAAGACCTCC CCAGCCCAGG GTCACGGGGC CAGCAGGAAC AGCCGGAAGG AACAACCCTA 
GTCAAGGAGG AAGCGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTACGAGACA 
AACTGCCACT GGGAAGGCTG CACCAGAGAG TTCGACACCC AAGATCAGCT TGTGCATCAT 
ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTCG TGTGCCGCTG GCTTGATTGT 
TCACGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTGCATAT GAGAAGACAC 
ACTGGGGAGA AGCCTCACAA ATGTACATTT GAAGGTTGCA CAAAAGCCTA CTCAAGACTC 
GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATACGT CTGTGAGCAT 
GAGGGCTGCA ACAAGGCTTT CTCTAATGCT TCAGATCGGG CCAAGCACCA AAACAGAACA 
CATTCCAATG AGAAACCGTA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC 
CCCAGCTCTC TCCGGAAACA CGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG 
AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CCCCTGGCCG GCCAACTCAG GGAGCATTTG GTGAGCAGAA GGAGCTGAGC 
AACACTACCT CAAAGCGGGA AGAGTGCCTC CAGGTGAAGA CTGTCAAGGC TGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TGTAGCAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CATCAAACTT 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA 
GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CCAATAACAA CTATAGCTCG 
GGTGGGCCCG GGACCCTCCT CCCGAGCAGA AGTGACCTGT CAGGTGTAGA CTTCACAGTG 
CTGAACACAC TCAACAGGAG AGACAGCAAT ACCAGCACTA TCAGCTCTGC CTACCTGAGC 
AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG CGAGGCGTCC 
CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCTGACT CCTACGATCC CATCTCCACA 
GATGCTTCAA GGAGGTCCAG CGAGGCCAGC CAGGGTGACG GGCTGCCCAG TCTGCTCAGC 
CTCACACCCG TGCAGCAGTA CCGCCTTAAG GCCAAGTATG CAGCTGCCAC AGGTGGCCCA 
CCACCCACAC CTCTGCCCCA CATGGAGAGG CTGAGCCTGA AGACCAAGAT GGCCTTGCTA 
GGGGAAGGGA GGGACTCTGG GGTGACCCTG CCTCCAGTCC ATCCTCCTCG AAGATGCAGT 
GATGGGGGAG GTCACACATA CAGAGGGCGT CACCTGATGC CTCATGATGC ACTAGCGAAC 
AGTGTGAGGA GAGCCAGCGA CCCTGTGAGG ACAGTCTCGG AGAACATGTC ACTTGCCAGG 
GTTCAACGCT TCAGCAGCCT CAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCGGTGGAA 
AAGCGTAGCT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCAGCCAACC CCGGTACTTC 
CAGGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC TCTGACCATG 
GATGCTGATG CTAACTTGAA TGATGAAGAC CTCCTGCCAG ATGATGTGGT ACAGTATTTA 
AATTCCCAGA ACCAAACAGG GTATGGGCAG CAGTTGCAGA GTGGCATCTC TGAAGACAGC 
AAAGTAGCCC ATGAGCCAGA GGACTTGGAC TTAGCAGGGC TGCCAGACAG TCATGTTGGC 
CAGGAGTACC CAGCTCTGGA GCAGCCCTGC TCTGAGGGCA GTAAAACTGA TTTGCCCATC 
CAGTGGAACG AGGTCAGTTC TGGAACTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT 
CAGCAGCGCC CTAGCGCACA GCAGCCTCGG GGTTTTGGGC TATACAACAA TATGGTGGTA 
CACCCACATA ATCTGTGGAA GGTTGGCACT GGCCCAGCTG GGGGATATCA GACCCTCGGG 
GAGAATAGCA GTACCTACAA TGGCCCAGAG CACTTTGCAA TCCACAGTGG GGATGGACTT 
GGCACCAATG GAAATACTTT CCATGAACAG CCCTTTAAGA CCCAGCAGTA TGGGAGCCAG 
CTCAACAGGC AGCCACTGAC TTCCAGTGCT CTAGATCATG CCTGTGGTAC AGGGATTCAA 
GGGTCCAAGC TAAAAGGCAA CAGCTTGCAA GAGAATGGGG GTTTGCTAGA TTTCAGCCTG 
TCCGTGGCAC CAAATGAGTT AGCTGGCAAC ACAGTGAATG GCATGCAAAC CCAAGATCAA 
ATGGGACAGG GATACATTGC CCATCAGCTA CTCAGTGGCA GCATGCAACA CCAGGGGCCC 
AGCCGCCCTG GTCAACAGGT ACTAGGGCAG GTTGGTGCTA CCTCACATAT CAACATCTAT 
CAAGGGACAG AGAGCTGCCT ACCAGGGACT CAGGACAACA GCAGCCAGCC ATCAAGCATG 
GCAGCTATCA GGGGCTACCA GCCCTGTGCC AGCTATGGGG GTAACAGGCG TCAGGCAATG 
CCAAGGGGCA ACCTCACTCT GCAACAAGGA CAGCTCAGTG ACATGAGTCA GAGCAGCAGG 
GTGAACAGCA TCAAAATGGA GGCACAAGGT CAGTCCCAGC AGCTCTGCTC TACCGTGCAG 
AATTATTCCG GTCAGTTCTA TGACCAAACC ATGGGCTTCA GTCAGCAAGA CAGGAAAGCT 
GGCTCGTTCT CCCTCTCAGA TGCCAACTGC CTGCTCCAAG GGAATGGCAC TGAAAACTCT 
GAGTTACTCT CCCCAGGTGC TAACCAGGTA ACAAGCACAG TTGACAGCTT TGAGAGTCAT 
GACCTAGAAG GTGTGCAGAT TGATTTTGAT GCCATCATAG ATGATGGGGA CCATACCAGC 
CTAATGTCAG GGGCCTTGAG CCCAAGTATT ATTCAGAACC TTTCCCACAG CTCCTCCCGT 
CTCACCACTC CGCGGGCATC CCTCCCATTC CCATCCCTAT CCATGGGCAC CACCAACATG 
GCTATCGGGG ATATGAGTTC TTTGCTGACC TCCCTTGCAG AAGAAAGCAA GTTCCTTGCA 
GTTATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032156.2
CDS	431..5182
Translation	MEAQAHSSTATERKKAENSIGKCPTRTDVSEKAVASSTTSNEDESPGQIYHRERRNAITMQPQSVQGLNKISEEPSTSSDERASLIKKEIHGSLPHLAEPSLPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADILPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQSKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGSRGQQEQPEGTTLVKEEADKDESKQEPEVIYETNCHWEGCTREFDTQDQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDMHPRPPPPRDSGSHSQSRSPGRPTQGAFGEQKELSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSVADLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHIKLERLKQVNGMFPRLNPILPSKAPAVSPLIGNGTQSNNNYSSGGPGTLLPSRSDLSGVDFTVLNTLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPVQQYRLKAKYAAATGGPPPTPLPHMERLSLKTKMALLGEGRDSGVTLPPVHPPRRCSDGGGHTYRGRHLMPHDALANSVRRASDPVRTVSENMSLARVQRFSSLNSFNPPNLPPSVEKRSLVLQNYTRQESSQPRYFQASPCPPSITENVALEALTMDADANLNDEDLLPDDVVQYLNSQNQTGYGQQLQSGISEDSKVAHEPEDLDLAGLPDSHVGQEYPALEQPCSEGSKTDLPIQWNEVSSGTSDLSSSKLKCGQQRPSAQQPRGFGLYNNMVVHPHNLWKVGTGPAGGYQTLGENSSTYNGPEHFAIHSGDGLGTNGNTFHEQPFKTQQYGSQLNRQPLTSSALDHACGTGIQGSKLKGNSLQENGGLLDFSLSVAPNELAGNTVNGMQTQDQMGQGYIAHQLLSGSMQHQGPSRPGQQVLGQVGATSHINIYQGTESCLPGTQDNSSQPSSMAAIRGYQPCASYGGNRRQAMPRGNLTLQQGQLSDMSQSSRVNSIKMEAQGQSQQLCSTVQNYSGQFYDQTMGFSQQDRKAGSFSLSDANCLLQGNGTENSELLSPGANQVTSTVDSFESHDLEGVQIDFDAIIDDGDHTSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPSLSMGTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	NM_008130.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008130.3

ORF Insert Sequence:

ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG 
CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT 
GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC 
TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT 
GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT 
AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT 
ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA 
GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG 
ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT 
GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC 
AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA 
GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT 
GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT 
CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC 
ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG 
AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGAGCAAGCC CACAAGCGAG 
TCTGCAGTGA GCAGCACTGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCTGAT 
GAAGACCTCC CCAGCCCAGG GTCACGGGGC CAGCAGGAAC AGCCGGAAGG AACAACCCTA 
GTCAAGGAGG AAGCGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTACGAGACA 
AACTGCCACT GGGAAGGCTG CACCAGAGAG TTCGACACCC AAGATCAGCT TGTGCATCAT 
ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTCG TGTGCCGCTG GCTTGATTGT 
TCACGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTGCATAT GAGAAGACAC 
ACTGGGGAGA AGCCTCACAA ATGTACATTT GAAGGTTGCA CAAAAGCCTA CTCAAGACTC 
GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATACGT CTGTGAGCAT 
GAGGGCTGCA ACAAGGCTTT CTCTAATGCT TCAGATCGGG CCAAGCACCA AAACAGAACA 
CATTCCAATG AGAAACCGTA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC 
CCCAGCTCTC TCCGGAAACA CGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG 
AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CCCCTGGCCG GCCAACTCAG GGAGCATTTG GTGAGCAGAA GGAGCTGAGC 
AACACTACCT CAAAGCGGGA AGAGTGCCTC CAGGTGAAGA CTGTCAAGGC TGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TGTAGCAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CATCAAACTT 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA 
GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CCAATAACAA CTATAGCTCG 
GGTGGGCCCG GGACCCTCCT CCCGAGCAGA AGTGACCTGT CAGGTGTAGA CTTCACAGTG 
CTGAACACAC TCAACAGGAG AGACAGCAAT ACCAGCACTA TCAGCTCTGC CTACCTGAGC 
AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG CGAGGCGTCC 
CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCTGACT CCTACGATCC CATCTCCACA 
GATGCTTCAA GGAGGTCCAG CGAGGCCAGC CAGGGTGACG GGCTGCCCAG TCTGCTCAGC 
CTCACACCCG TGCAGCAGTA CCGCCTTAAG GCCAAGTATG CAGCTGCCAC AGGTGGCCCA 
CCACCCACAC CTCTGCCCCA CATGGAGAGG CTGAGCCTGA AGACCAAGAT GGCCTTGCTA 
GGGGAAGGGA GGGACTCTGG GGTGACCCTG CCTCCAGTCC ATCCTCCTCG AAGATGCAGT 
GATGGGGGAG GTCACACATA CAGAGGGCGT CACCTGATGC CTCATGATGC ACTAGCGAAC 
AGTGTGAGGA GAGCCAGCGA CCCTGTGAGG ACAGTCTCGG AGAACATGTC ACTTGCCAGG 
GTTCAACGCT TCAGCAGCCT CAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCGGTGGAA 
AAGCGTAGCT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCAGCCAACC CCGGTACTTC 
CAGGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC TCTGACCATG 
GATGCTGATG CTAACTTGAA TGATGAAGAC CTCCTGCCAG ATGATGTGGT ACAGTATTTA 
AATTCCCAGA ACCAAACAGG GTATGGGCAG CAGTTGCAGA GTGGCATCTC TGAAGACAGC 
AAAGTAGCCC ATGAGCCAGA GGACTTGGAC TTAGCAGGGC TGCCAGACAG TCATGTTGGC 
CAGGAGTACC CAGCTCTGGA GCAGCCCTGC TCTGAGGGCA GTAAAACTGA TTTGCCCATC 
CAGTGGAACG AGGTCAGTTC TGGAACTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT 
CAGCAGCGCC CTAGCGCACA GCAGCCTCGG GGTTTTGGGC TATACAACAA TATGGTGGTA 
CACCCACATA ATCTGTGGAA GGTTGGCACT GGCCCAGCTG GGGGATATCA GACCCTCGGG 
GAGAATAGCA GTACCTACAA TGGCCCAGAG CACTTTGCAA TCCACAGTGG GGATGGACTT 
GGCACCAATG GAAATACTTT CCATGAACAG CCCTTTAAGA CCCAGCAGTA TGGGAGCCAG 
CTCAACAGGC AGCCACTGAC TTCCAGTGCT CTAGATCATG CCTGTGGTAC AGGGATTCAA 
GGGTCCAAGC TAAAAGGCAA CAGCTTGCAA GAGAATGGGG GTTTGCTAGA TTTCAGCCTG 
TCCGTGGCAC CAAATGAGTT AGCTGGCAAC ACAGTGAATG GCATGCAAAC CCAAGATCAA 
ATGGGACAGG GATACATTGC CCATCAGCTA CTCAGTGGCA GCATGCAACA CCAGGGGCCC 
AGCCGCCCTG GTCAACAGGT ACTAGGGCAG GTTGGTGCTA CCTCACATAT CAACATCTAT 
CAAGGGACAG AGAGCTGCCT ACCAGGGACT CAGGACAACA GCAGCCAGCC ATCAAGCATG 
GCAGCTATCA GGGGCTACCA GCCCTGTGCC AGCTATGGGG GTAACAGGCG TCAGGCAATG 
CCAAGGGGCA ACCTCACTCT GCAACAAGGA CAGCTCAGTG ACATGAGTCA GAGCAGCAGG 
GTGAACAGCA TCAAAATGGA GGCACAAGGT CAGTCCCAGC AGCTCTGCTC TACCGTGCAG 
AATTATTCCG GTCAGTTCTA TGACCAAACC ATGGGCTTCA GTCAGCAAGA CAGGAAAGCT 
GGCTCGTTCT CCCTCTCAGA TGCCAACTGC CTGCTCCAAG GGAATGGCAC TGAAAACTCT 
GAGTTACTCT CCCCAGGTGC TAACCAGGTA ACAAGCACAG TTGACAGCTT TGAGAGTCAT 
GACCTAGAAG GTGTGCAGAT TGATTTTGAT GCCATCATAG ATGATGGGGA CCATACCAGC 
CTAATGTCAG GGGCCTTGAG CCCAAGTATT ATTCAGAACC TTTCCCACAG CTCCTCCCGT 
CTCACCACTC CGCGGGCATC CCTCCCATTC CCATCCCTAT CCATGGGCAC CACCAACATG 
GCTATCGGGG ATATGAGTTC TTTGCTGACC TCCCTTGCAG AAGAAAGCAA GTTCCTTGCA 
GTTATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli3 ( XM_006516552.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516615.1 Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X1, mRNA.

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CloneID	OMu48321
Clone ID Related Accession (Same CDS sequence)	XM_006516552.3
Accession Version	XM_006516552.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG 
CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT 
GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC 
TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT 
GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT 
AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT 
ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA 
GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG 
ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT 
GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC 
AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA 
GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT 
GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT 
CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC 
ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG 
AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGCAGAGCAA GCCCACAAGC 
GAGTCTGCAG TGAGCAGCAC TGGTGACCCT ATGCATAATA AGCGGTCCAA GATCAAGCCT 
GATGAAGACC TCCCCAGCCC AGGGTCACGG GGCCAGCAGG AACAGCCGGA AGGAACAACC 
CTAGTCAAGG AGGAAGCGGA CAAAGATGAA AGCAAGCAGG AGCCTGAAGT CATCTACGAG 
ACAAACTGCC ACTGGGAAGG CTGCACCAGA GAGTTCGACA CCCAAGATCA GCTTGTGCAT 
CATATCAATA ATGACCACAT TCATGGAGAA AAGAAGGAGT TCGTGTGCCG CTGGCTTGAT 
TGTTCACGAG AGCAGAAACC GTTCAAAGCC CAGTACATGT TGGTAGTGCA TATGAGAAGA 
CACACTGGGG AGAAGCCTCA CAAATGTACA TTTGAAGGTT GCACAAAAGC CTACTCAAGA 
CTCGAAAACC TGAAAACCCA CTTGAGATCT CACACTGGAG AGAAGCCATA CGTCTGTGAG 
CATGAGGGCT GCAACAAGGC TTTCTCTAAT GCTTCAGATC GGGCCAAGCA CCAAAACAGA 
ACACATTCCA ATGAGAAACC GTATGTATGC AAAATCCCTG GCTGCACCAA GCGTTACACA 
GACCCCAGCT CTCTCCGGAA ACACGTGAAG ACTGTGCATG GCCCTGAGGC TCATGTTACC 
AAGAAGCAGC GTGGGGACAT GCACCCTCGG CCTCCACCCC CGAGAGATTC CGGCAGCCAT 
TCACAGTCCA GGTCCCCTGG CCGGCCAACT CAGGGAGCAT TTGGTGAGCA GAAGGAGCTG 
AGCAACACTA CCTCAAAGCG GGAAGAGTGC CTCCAGGTGA AGACTGTCAA GGCTGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CTGATGGAGG TAGTGTAGCA 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC GGCAACCACA 
GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACATCAAA 
CTTGAAAGGC TAAAACAAGT GAATGGAATG TTTCCAAGAC TGAACCCTAT TCTACCCTCC 
AAAGCCCCTG CGGTATCTCC TCTCATAGGA AATGGCACAC AGTCCAATAA CAACTATAGC 
TCGGGTGGGC CCGGGACCCT CCTCCCGAGC AGAAGTGACC TGTCAGGTGT AGACTTCACA 
GTGCTGAACA CACTCAACAG GAGAGACAGC AATACCAGCA CTATCAGCTC TGCCTACCTG 
AGCAGCCGCA GATCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCAGGTC CAGCGAGGCG 
TCCCAGGCTG AAGGGCGACC CCAGAATGTG AGTGTGGCTG ACTCCTACGA TCCCATCTCC 
ACAGATGCTT CAAGGAGGTC CAGCGAGGCC AGCCAGGGTG ACGGGCTGCC CAGTCTGCTC 
AGCCTCACAC CCGTGCAGCA GTACCGCCTT AAGGCCAAGT ATGCAGCTGC CACAGGTGGC 
CCACCACCCA CACCTCTGCC CCACATGGAG AGGCTGAGCC TGAAGACCAA GATGGCCTTG 
CTAGGGGAAG GGAGGGACTC TGGGGTGACC CTGCCTCCAG TCCATCCTCC TCGAAGATGC 
AGTGATGGGG GAGGTCACAC ATACAGAGGG CGTCACCTGA TGCCTCATGA TGCACTAGCG 
AACAGTGTGA GGAGAGCCAG CGACCCTGTG AGGACAGTCT CGGAGAACAT GTCACTTGCC 
AGGGTTCAAC GCTTCAGCAG CCTCAATAGC TTTAATCCTC CAAATTTGCC TCCATCGGTG 
GAAAAGCGTA GCTTAGTGCT GCAAAATTAT ACCCGGCAGG AGAGCAGCCA ACCCCGGTAC 
TTCCAGGCAT CCCCTTGCCC TCCCAGCATC ACAGAGAATG TTGCCCTGGA GGCTCTGACC 
ATGGATGCTG ATGCTAACTT GAATGATGAA GACCTCCTGC CAGATGATGT GGTACAGTAT 
TTAAATTCCC AGAACCAAAC AGGGTATGGG CAGCAGTTGC AGAGTGGCAT CTCTGAAGAC 
AGCAAAGTAG CCCATGAGCC AGAGGACTTG GACTTAGCAG GGCTGCCAGA CAGTCATGTT 
GGCCAGGAGT ACCCAGCTCT GGAGCAGCCC TGCTCTGAGG GCAGTAAAAC TGATTTGCCC 
ATCCAGTGGA ACGAGGTCAG TTCTGGAACT TCTGATCTGT CATCCTCCAA GCTGAAGTGT 
GGTCAGCAGC GCCCTAGCGC ACAGCAGCCT CGGGGTTTTG GGCTATACAA CAATATGGTG 
GTACACCCAC ATAATCTGTG GAAGGTTGGC ACTGGCCCAG CTGGGGGATA TCAGACCCTC 
GGGGAGAATA GCAGTACCTA CAATGGCCCA GAGCACTTTG CAATCCACAG TGGGGATGGA 
CTTGGCACCA ATGGAAATAC TTTCCATGAA CAGCCCTTTA AGACCCAGCA GTATGGGAGC 
CAGCTCAACA GGCAGCCACT GACTTCCAGT GCTCTAGATC ATGCCTGTGG TACAGGGATT 
CAAGGGTCCA AGCTAAAAGG CAACAGCTTG CAAGAGAATG GGGGTTTGCT AGATTTCAGC 
CTGTCCGTGG CACCAAATGA GTTAGCTGGC AACACAGTGA ATGGCATGCA AACCCAAGAT 
CAAATGGGAC AGGGATACAT TGCCCATCAG CTACTCAGTG GCAGCATGCA ACACCAGGGG 
CCCAGCCGCC CTGGTCAACA GGTACTAGGG CAGGTTGGTG CTACCTCACA TATCAACATC 
TATCAAGGGA CAGAGAGCTG CCTACCAGGG ACTCAGGACA ACAGCAGCCA GCCATCAAGC 
ATGGCAGCTA TCAGGGGCTA CCAGCCCTGT GCCAGCTATG GGGGTAACAG GCGTCAGGCA 
ATGCCAAGGG GCAACCTCAC TCTGCAACAA GGACAGCTCA GTGACATGAG TCAGAGCAGC 
AGGGTGAACA GCATCAAAAT GGAGGCACAA GGTCAGTCCC AGCAGCTCTG CTCTACCGTG 
CAGAATTATT CCGGTCAGTT CTATGACCAA ACCATGGGCT TCAGTCAGCA AGACAGGAAA 
GCTGGCTCGT TCTCCCTCTC AGATGCCAAC TGCCTGCTCC AAGGGAATGG CACTGAAAAC 
TCTGAGTTAC TCTCCCCAGG TGCTAACCAG GTAACAAGCA CAGTTGACAG CTTTGAGAGT 
CATGACCTAG AAGGTGTGCA GATTGATTTT GATGCCATCA TAGATGATGG GGACCATACC 
AGCCTAATGT CAGGGGCCTT GAGCCCAAGT ATTATTCAGA ACCTTTCCCA CAGCTCCTCC 
CGTCTCACCA CTCCGCGGGC ATCCCTCCCA TTCCCATCCC TATCCATGGG CACCACCAAC 
ATGGCTATCG GGGATATGAG TTCTTTGCTG ACCTCCCTTG CAGAAGAAAG CAAGTTCCTT 
GCAGTTATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516615.1
CDS	130..4884
Translation	MEAQAHSSTATERKKAENSIGKCPTRTDVSEKAVASSTTSNEDESPGQIYHRERRNAITMQPQSVQGLNKISEEPSTSSDERASLIKKEIHGSLPHLAEPSLPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADILPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQQSKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGSRGQQEQPEGTTLVKEEADKDESKQEPEVIYETNCHWEGCTREFDTQDQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDMHPRPPPPRDSGSHSQSRSPGRPTQGAFGEQKELSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSVADLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHIKLERLKQVNGMFPRLNPILPSKAPAVSPLIGNGTQSNNNYSSGGPGTLLPSRSDLSGVDFTVLNTLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPVQQYRLKAKYAAATGGPPPTPLPHMERLSLKTKMALLGEGRDSGVTLPPVHPPRRCSDGGGHTYRGRHLMPHDALANSVRRASDPVRTVSENMSLARVQRFSSLNSFNPPNLPPSVEKRSLVLQNYTRQESSQPRYFQASPCPPSITENVALEALTMDADANLNDEDLLPDDVVQYLNSQNQTGYGQQLQSGISEDSKVAHEPEDLDLAGLPDSHVGQEYPALEQPCSEGSKTDLPIQWNEVSSGTSDLSSSKLKCGQQRPSAQQPRGFGLYNNMVVHPHNLWKVGTGPAGGYQTLGENSSTYNGPEHFAIHSGDGLGTNGNTFHEQPFKTQQYGSQLNRQPLTSSALDHACGTGIQGSKLKGNSLQENGGLLDFSLSVAPNELAGNTVNGMQTQDQMGQGYIAHQLLSGSMQHQGPSRPGQQVLGQVGATSHINIYQGTESCLPGTQDNSSQPSSMAAIRGYQPCASYGGNRRQAMPRGNLTLQQGQLSDMSQSSRVNSIKMEAQGQSQQLCSTVQNYSGQFYDQTMGFSQQDRKAGSFSLSDANCLLQGNGTENSELLSPGANQVTSTVDSFESHDLEGVQIDFDAIIDDGDHTSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPSLSMGTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_006516552.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516552.3

ORF Insert Sequence:

ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG 
CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT 
GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC 
TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT 
GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT 
AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT 
ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA 
GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG 
ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT 
GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC 
AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA 
GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT 
GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT 
CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC 
ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG 
AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGCAGAGCAA GCCCACAAGC 
GAGTCTGCAG TGAGCAGCAC TGGTGACCCT ATGCATAATA AGCGGTCCAA GATCAAGCCT 
GATGAAGACC TCCCCAGCCC AGGGTCACGG GGCCAGCAGG AACAGCCGGA AGGAACAACC 
CTAGTCAAGG AGGAAGCGGA CAAAGATGAA AGCAAGCAGG AGCCTGAAGT CATCTACGAG 
ACAAACTGCC ACTGGGAAGG CTGCACCAGA GAGTTCGACA CCCAAGATCA GCTTGTGCAT 
CATATCAATA ATGACCACAT TCATGGAGAA AAGAAGGAGT TCGTGTGCCG CTGGCTTGAT 
TGTTCACGAG AGCAGAAACC GTTCAAAGCC CAGTACATGT TGGTAGTGCA TATGAGAAGA 
CACACTGGGG AGAAGCCTCA CAAATGTACA TTTGAAGGTT GCACAAAAGC CTACTCAAGA 
CTCGAAAACC TGAAAACCCA CTTGAGATCT CACACTGGAG AGAAGCCATA CGTCTGTGAG 
CATGAGGGCT GCAACAAGGC TTTCTCTAAT GCTTCAGATC GGGCCAAGCA CCAAAACAGA 
ACACATTCCA ATGAGAAACC GTATGTATGC AAAATCCCTG GCTGCACCAA GCGTTACACA 
GACCCCAGCT CTCTCCGGAA ACACGTGAAG ACTGTGCATG GCCCTGAGGC TCATGTTACC 
AAGAAGCAGC GTGGGGACAT GCACCCTCGG CCTCCACCCC CGAGAGATTC CGGCAGCCAT 
TCACAGTCCA GGTCCCCTGG CCGGCCAACT CAGGGAGCAT TTGGTGAGCA GAAGGAGCTG 
AGCAACACTA CCTCAAAGCG GGAAGAGTGC CTCCAGGTGA AGACTGTCAA GGCTGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CTGATGGAGG TAGTGTAGCA 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC GGCAACCACA 
GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACATCAAA 
CTTGAAAGGC TAAAACAAGT GAATGGAATG TTTCCAAGAC TGAACCCTAT TCTACCCTCC 
AAAGCCCCTG CGGTATCTCC TCTCATAGGA AATGGCACAC AGTCCAATAA CAACTATAGC 
TCGGGTGGGC CCGGGACCCT CCTCCCGAGC AGAAGTGACC TGTCAGGTGT AGACTTCACA 
GTGCTGAACA CACTCAACAG GAGAGACAGC AATACCAGCA CTATCAGCTC TGCCTACCTG 
AGCAGCCGCA GATCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCAGGTC CAGCGAGGCG 
TCCCAGGCTG AAGGGCGACC CCAGAATGTG AGTGTGGCTG ACTCCTACGA TCCCATCTCC 
ACAGATGCTT CAAGGAGGTC CAGCGAGGCC AGCCAGGGTG ACGGGCTGCC CAGTCTGCTC 
AGCCTCACAC CCGTGCAGCA GTACCGCCTT AAGGCCAAGT ATGCAGCTGC CACAGGTGGC 
CCACCACCCA CACCTCTGCC CCACATGGAG AGGCTGAGCC TGAAGACCAA GATGGCCTTG 
CTAGGGGAAG GGAGGGACTC TGGGGTGACC CTGCCTCCAG TCCATCCTCC TCGAAGATGC 
AGTGATGGGG GAGGTCACAC ATACAGAGGG CGTCACCTGA TGCCTCATGA TGCACTAGCG 
AACAGTGTGA GGAGAGCCAG CGACCCTGTG AGGACAGTCT CGGAGAACAT GTCACTTGCC 
AGGGTTCAAC GCTTCAGCAG CCTCAATAGC TTTAATCCTC CAAATTTGCC TCCATCGGTG 
GAAAAGCGTA GCTTAGTGCT GCAAAATTAT ACCCGGCAGG AGAGCAGCCA ACCCCGGTAC 
TTCCAGGCAT CCCCTTGCCC TCCCAGCATC ACAGAGAATG TTGCCCTGGA GGCTCTGACC 
ATGGATGCTG ATGCTAACTT GAATGATGAA GACCTCCTGC CAGATGATGT GGTACAGTAT 
TTAAATTCCC AGAACCAAAC AGGGTATGGG CAGCAGTTGC AGAGTGGCAT CTCTGAAGAC 
AGCAAAGTAG CCCATGAGCC AGAGGACTTG GACTTAGCAG GGCTGCCAGA CAGTCATGTT 
GGCCAGGAGT ACCCAGCTCT GGAGCAGCCC TGCTCTGAGG GCAGTAAAAC TGATTTGCCC 
ATCCAGTGGA ACGAGGTCAG TTCTGGAACT TCTGATCTGT CATCCTCCAA GCTGAAGTGT 
GGTCAGCAGC GCCCTAGCGC ACAGCAGCCT CGGGGTTTTG GGCTATACAA CAATATGGTG 
GTACACCCAC ATAATCTGTG GAAGGTTGGC ACTGGCCCAG CTGGGGGATA TCAGACCCTC 
GGGGAGAATA GCAGTACCTA CAATGGCCCA GAGCACTTTG CAATCCACAG TGGGGATGGA 
CTTGGCACCA ATGGAAATAC TTTCCATGAA CAGCCCTTTA AGACCCAGCA GTATGGGAGC 
CAGCTCAACA GGCAGCCACT GACTTCCAGT GCTCTAGATC ATGCCTGTGG TACAGGGATT 
CAAGGGTCCA AGCTAAAAGG CAACAGCTTG CAAGAGAATG GGGGTTTGCT AGATTTCAGC 
CTGTCCGTGG CACCAAATGA GTTAGCTGGC AACACAGTGA ATGGCATGCA AACCCAAGAT 
CAAATGGGAC AGGGATACAT TGCCCATCAG CTACTCAGTG GCAGCATGCA ACACCAGGGG 
CCCAGCCGCC CTGGTCAACA GGTACTAGGG CAGGTTGGTG CTACCTCACA TATCAACATC 
TATCAAGGGA CAGAGAGCTG CCTACCAGGG ACTCAGGACA ACAGCAGCCA GCCATCAAGC 
ATGGCAGCTA TCAGGGGCTA CCAGCCCTGT GCCAGCTATG GGGGTAACAG GCGTCAGGCA 
ATGCCAAGGG GCAACCTCAC TCTGCAACAA GGACAGCTCA GTGACATGAG TCAGAGCAGC 
AGGGTGAACA GCATCAAAAT GGAGGCACAA GGTCAGTCCC AGCAGCTCTG CTCTACCGTG 
CAGAATTATT CCGGTCAGTT CTATGACCAA ACCATGGGCT TCAGTCAGCA AGACAGGAAA 
GCTGGCTCGT TCTCCCTCTC AGATGCCAAC TGCCTGCTCC AAGGGAATGG CACTGAAAAC 
TCTGAGTTAC TCTCCCCAGG TGCTAACCAG GTAACAAGCA CAGTTGACAG CTTTGAGAGT 
CATGACCTAG AAGGTGTGCA GATTGATTTT GATGCCATCA TAGATGATGG GGACCATACC 
AGCCTAATGT CAGGGGCCTT GAGCCCAAGT ATTATTCAGA ACCTTTCCCA CAGCTCCTCC 
CGTCTCACCA CTCCGCGGGC ATCCCTCCCA TTCCCATCCC TATCCATGGG CACCACCAAC 
ATGGCTATCG GGGATATGAG TTCTTTGCTG ACCTCCCTTG CAGAAGAAAG CAAGTTCCTT 
GCAGTTATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Primary cilia in murine palatal rugae development.
Gene expression patterns : GEP34119062(2019 12)
Nakaniwa M,Kawasaki M,Kawasaki K,Yamada A,Meguro F,Takeyasu M,Ohazama A

Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals.
Nature communications10(1)4647(2019 10)
Yung T,Poon F,Liang M,Coquenlorge S,McGaugh EC,Hui CC,Wilson MD,Nostro MC,Kim TH

Derepression of sonic hedgehog signaling upon Gpr161 deletion unravels forebrain and ventricular abnormalities.
Developmental biology450(1)47-62(2019 06)
Shimada IS,Somatilaka BN,Hwang SH,Anderson AG,Shelton JM,Rajaram V,Konopka G,Mukhopadhyay S

GLI3 repressor but not GLI3 activator is essential for mouse eye patterning and morphogenesis.
Developmental biology450(2)141-154(2019 06)
Wiegering A,Petzsch P,Köhrer K,Rüther U,Gerhardt C

Secreted metalloproteases ADAMTS9 and ADAMTS20 have a non-canonical role in ciliary vesicle growth during ciliogenesis.
Nature communications10(1)953(2019 02)
Nandadasa S,Kraft CM,Wang LW,O'Donnell A,Patel R,Gee HY,Grobe K,Cox TC,Hildebrandt F,Apte SS

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Gli3 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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