gli3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gli3
Entrez Gene ID	403042
Full Name	GLI family zinc finger 3
Synonyms	im:7142603
General protein information	Preferred Names GLI family zinc finger 3 Names transcriptional activator GLI3 GLI-Kruppel family member GLI3 zinc finger transcription factor Gli3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205728.1	NP_991291.1	transcriptional activator GLI3
XM_021470118.1	XP_021325793.1	transcriptional activator GLI3 isoform X1
XM_021470119.1	XP_021325794.1	transcriptional activator GLI3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
Macaca mulatta (Rhesus monkey)	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Gene Ontology
Bibliography

Related articles in PubMed

let-7 MicroRNA-Mediated Regulation of Shh Signaling and the Gene Regulatory Network Is Essential for Retina Regeneration.
Kaur S, Gupta S, Chaudhary M, Khursheed MA, Mitra S, Kurup AJ, Ramachandran R
Cell reports23(5)1409-1423(2018 05)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Molecular mechanisms underlying the exceptional adaptations of batoid fins.
Nakamura T, Klomp J, Pieretti J, Schneider I, Gehrke AR, Shubin NH
Proceedings of the National Academy of Sciences of the United States of America112(52)15940-5(2015 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Sox4 regulates choroid fissure closure by limiting Hedgehog signaling during ocular morphogenesis.
Wen W, Pillai-Kastoori L, Wilson SG, Morris AC
Developmental biology399(1)139-153(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gli3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gli3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151662	XM_021470118.1 Latest version!	Danio rerio GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa151663	XM_021470119.1 Latest version!	Danio rerio GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa03571	NM_205728.1 Latest version!	Danio rerio GLI family zinc finger 3 (gli3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gli3 ( XM_021470118.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325793.1 Danio rerio GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151662
Clone ID Related Accession (Same CDS sequence)	XM_021470118.1
Accession Version	XM_021470118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCAA GTCAAGTCAA TCAAAACAGA AAAGCCCATG TGGGTGATCA GACGTCTCAG 
TCAAGCCCTG GCAGTCAGTC TTCATGCAGC AGTGACCAGT CCATCAACGG CCCCCATGTC 
AGCCAGGGAG TCCAGCTTAC TGTGAACAGT ATTGGGCTTG CGGGGGAGGT GGCAATGCTT 
GAGGACATGG AAAATGAAGA GGAGGATGAG GAGAACAGAG AAAGAGAGAA GGAGGATGCC 
CCCATTATGG ACTCTACAGT ATCCACAGCA ACATCCTCTG CGGCAACAAT AGTTTTGCAG 
ACTCATCGAA GTGTTGGACG GCCCCTACGC TGGATGGAGC ATGTTAAGAT GGAGAGGTTA 
AGGCAGGTGA ATGGAGCTGT GCCTAGGCTA GGATTCCTGC CTCCAACACC GCCCCCTAAA 
GGCCAGGCCA TGCCTGCCCT CACAGGAAAG GGTCAATGCC TTGGGAGGAG TACTTCTGTG 
AGGGTGCCTC CATGCCCTGA GCCGTCCAAC ACAGAGCTGA CAATTCTGAG TTTGCTGCAT 
GAACGACGGG ACAGTAGTGG CAGCAACACT AGCTCAGCTT ACCTGAGCAG CAGCCGTCGC 
TCATCTGGCA TCTCCCCCTG CTTTTCCAGT CGCTGTTCTA GCCAGGCTTC ACTGTCAGAG 
TACAGCCCCC ATCGACATAT CCATAATCTC AGTGCAACTG ATTCTTATGA CCCTATTTCC 
ACTGATGCCT CTCGACGCTC CAGTGAAGCC AGCCAGTATG ATGGAGGAAG TTCAAGTGCA 
GTGGTGTTTG GTGGAAGTGG AGTTTTCAAG GGCAGTGGTA TGGGATCAAG AGGGGATCTG 
AACCTCACTC CAGCTCAACA CTATAGCCTA AAGGCCAAGT ATGCAGCAGC AACAGGTGGA 
CCGCCTCCCA CACCCTTGCC CAGTATGGGG CATATAAACC TAAAGAGTCA CTTTGCTTTA 
AAGGAAGACA GAAAGGACAC TGGCCAGCTT ACACTACCAC CACTGGTCAG GTCACGTCGC 
TGTAGTGATG GTGCACACAA CTCGCATGAC AGTCAAGCCG TGTATCAACG AAAGGGAACA 
TACTTTGGTG GTGGTCCAGG GAAAAATGAT CGAAGAGCTA GTGATCCTAC ACATCACAAA 
CAAAATCTTA GTTCTTATGG CTTGCCACAA GTACGACATT TCAGTAGTTT AAACAATATG 
ACTTCATCAC CCAATCCTGC TAGTTATGGT TACTTAAACA GAGAAGATCA CAGTCTGAAC 
CTACAGAATT CCACCTGTCA CGTAGATAGC TTTCAGAGTG GTCTGCACTC CCCTTGTCCT 
CCTAGCATCA TGGAGCAATC AGCACTTGAG AACATTTCCA TAAACCAGGA TGGTCTTTTG 
TTACGTGTCA ATGAAAACAT CCAGTCTGAC TTCATGCAGT ATCTCCATTC CCAAGATCAG 
GAATCAATAA TTAACCAAGA CAACCATAGG CCTATTTTCA ATCCCGCAAA TGACCAGAAC 
TTCCTCAGTC CACAGCAGCA CCAAGAGGGA GTAGAGCAAG AAGACACTAG TATTGATCTG 
ATGCCTATCC AGTCGAATGA AGTGAGCTCT GGTAGTGCCG ATGTTACCAT TCCAAGGCAG 
CCGTTTCAGA GGTGTGGCAG ATGGTCAGAC CAGAGTGGCT CAGTAAGCAA CGCCTTTGGT 
CGATTTGGAA ACATGGTTGT ACAACAGCAG CCTATAGATT TTATTGATCA GCACTCAGCT 
CTTCAACACA TGGGAGGTAT AAGCTATGGG TGCAGAAATT CAGAGCAGCA TCTACAAGTA 
GCAAAACCAG CCCAAGAGAA GTCCAGGATG AAAACAGGCA TTAAGACTGA GTCTTCCTCA 
ACCTTCCAAC TGGAATCCAA ACACAGCAAC TTTGGTCGAC CAGACTTCTC TCATATCTTT 
TCGGCCCCCA TTCATCAAAA ATGTTCCCAG CATACAGTTG TATCCCAATC TTATCCCCAA 
CAAAATCAAA TTACAAATCA AGATAGCAGC AACCTCTTTT TGCAAAGACC AGGTAACTGC 
AGCTTACTCT TGGCTTACCG GCCTCAGCCA GCTGTCAACA CTTCCTACTC CACTAGGCAG 
GATATTAACA GCCATAATGT ACAATCATGC CATCCACAAA GCTTGAACAA TAGACTAGGC 
ACAAATTGTT CCCCTTCAAA TCAGGTTTCC GTTGTGAAGC TTGATCAAGT TAATCACCTT 
CAAATCAACT CTTGCTCATC AATTCAGCAA CAAACACCTG TGTTTATTGA TAAGTGTTTT 
GACCTAGAAA AGTCCAAGCT CTCTCACGCC ATAGTACAGT CATGTCTAAT GGAATCATTG 
ATGGCAGAGA AAGAAAGAAC TTCCAAAGTT CTTCTGTCAT CTGGGGTTGG TCAGGTGACT 
AGCACAGTAG AAAGAAGCCC AGCTGAAAAA GTTGGTTTAG GAATTTGTTC CATTGGTGAT 
GATAGTTATG ATCAGGGCAG TGTAGTGTCT TCCTCTCAAC TCAGTAATTC TCATGATTCC 
TTTGCATTTC ACAGTGTGAA TCCAGGCACT AACAACATGG CTATTAGTGA CATGAGCTCC 
CTCCTCACCA CCCTCGCAGA AGAGAGTAAA TTTCTGGCAA TCATCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325793.1
CDS	145..2775
Translation	MSASQVNQNRKAHVGDQTSQSSPGSQSSCSSDQSINGPHVSQGVQLTVNSIGLAGEVAMLEDMENEEEDEENREREKEDAPIMDSTVSTATSSAATIVLQTHRSVGRPLRWMEHVKMERLRQVNGAVPRLGFLPPTPPPKGQAMPALTGKGQCLGRSTSVRVPPCPEPSNTELTILSLLHERRDSSGSNTSSAYLSSSRRSSGISPCFSSRCSSQASLSEYSPHRHIHNLSATDSYDPISTDASRRSSEASQYDGGSSSAVVFGGSGVFKGSGMGSRGDLNLTPAQHYSLKAKYAAATGGPPPTPLPSMGHINLKSHFALKEDRKDTGQLTLPPLVRSRRCSDGAHNSHDSQAVYQRKGTYFGGGPGKNDRRASDPTHHKQNLSSYGLPQVRHFSSLNNMTSSPNPASYGYLNREDHSLNLQNSTCHVDSFQSGLHSPCPPSIMEQSALENISINQDGLLLRVNENIQSDFMQYLHSQDQESIINQDNHRPIFNPANDQNFLSPQQHQEGVEQEDTSIDLMPIQSNEVSSGSADVTIPRQPFQRCGRWSDQSGSVSNAFGRFGNMVVQQQPIDFIDQHSALQHMGGISYGCRNSEQHLQVAKPAQEKSRMKTGIKTESSSTFQLESKHSNFGRPDFSHIFSAPIHQKCSQHTVVSQSYPQQNQITNQDSSNLFLQRPGNCSLLLAYRPQPAVNTSYSTRQDINSHNVQSCHPQSLNNRLGTNCSPSNQVSVVKLDQVNHLQINSCSSIQQQTPVFIDKCFDLEKSKLSHAIVQSCLMESLMAEKERTSKVLLSSGVGQVTSTVERSPAEKVGLGICSIGDDSYDQGSVVSSSQLSNSHDSFAFHSVNPGTNNMAISDMSSLLTTLAEESKFLAIIQ

Target ORF information:

RefSeq Version	XM_021470118.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLI family zinc finger 3 (gli3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470118.1

ORF Insert Sequence:

ATGTCTGCAA GTCAAGTCAA TCAAAACAGA AAAGCCCATG TGGGTGATCA GACGTCTCAG 
TCAAGCCCTG GCAGTCAGTC TTCATGCAGC AGTGACCAGT CCATCAACGG CCCCCATGTC 
AGCCAGGGAG TCCAGCTTAC TGTGAACAGT ATTGGGCTTG CGGGGGAGGT GGCAATGCTT 
GAGGACATGG AAAATGAAGA GGAGGATGAG GAGAACAGAG AAAGAGAGAA GGAGGATGCC 
CCCATTATGG ACTCTACAGT ATCCACAGCA ACATCCTCTG CGGCAACAAT AGTTTTGCAG 
ACTCATCGAA GTGTTGGACG GCCCCTACGC TGGATGGAGC ATGTTAAGAT GGAGAGGTTA 
AGGCAGGTGA ATGGAGCTGT GCCTAGGCTA GGATTCCTGC CTCCAACACC GCCCCCTAAA 
GGCCAGGCCA TGCCTGCCCT CACAGGAAAG GGTCAATGCC TTGGGAGGAG TACTTCTGTG 
AGGGTGCCTC CATGCCCTGA GCCGTCCAAC ACAGAGCTGA CAATTCTGAG TTTGCTGCAT 
GAACGACGGG ACAGTAGTGG CAGCAACACT AGCTCAGCTT ACCTGAGCAG CAGCCGTCGC 
TCATCTGGCA TCTCCCCCTG CTTTTCCAGT CGCTGTTCTA GCCAGGCTTC ACTGTCAGAG 
TACAGCCCCC ATCGACATAT CCATAATCTC AGTGCAACTG ATTCTTATGA CCCTATTTCC 
ACTGATGCCT CTCGACGCTC CAGTGAAGCC AGCCAGTATG ATGGAGGAAG TTCAAGTGCA 
GTGGTGTTTG GTGGAAGTGG AGTTTTCAAG GGCAGTGGTA TGGGATCAAG AGGGGATCTG 
AACCTCACTC CAGCTCAACA CTATAGCCTA AAGGCCAAGT ATGCAGCAGC AACAGGTGGA 
CCGCCTCCCA CACCCTTGCC CAGTATGGGG CATATAAACC TAAAGAGTCA CTTTGCTTTA 
AAGGAAGACA GAAAGGACAC TGGCCAGCTT ACACTACCAC CACTGGTCAG GTCACGTCGC 
TGTAGTGATG GTGCACACAA CTCGCATGAC AGTCAAGCCG TGTATCAACG AAAGGGAACA 
TACTTTGGTG GTGGTCCAGG GAAAAATGAT CGAAGAGCTA GTGATCCTAC ACATCACAAA 
CAAAATCTTA GTTCTTATGG CTTGCCACAA GTACGACATT TCAGTAGTTT AAACAATATG 
ACTTCATCAC CCAATCCTGC TAGTTATGGT TACTTAAACA GAGAAGATCA CAGTCTGAAC 
CTACAGAATT CCACCTGTCA CGTAGATAGC TTTCAGAGTG GTCTGCACTC CCCTTGTCCT 
CCTAGCATCA TGGAGCAATC AGCACTTGAG AACATTTCCA TAAACCAGGA TGGTCTTTTG 
TTACGTGTCA ATGAAAACAT CCAGTCTGAC TTCATGCAGT ATCTCCATTC CCAAGATCAG 
GAATCAATAA TTAACCAAGA CAACCATAGG CCTATTTTCA ATCCCGCAAA TGACCAGAAC 
TTCCTCAGTC CACAGCAGCA CCAAGAGGGA GTAGAGCAAG AAGACACTAG TATTGATCTG 
ATGCCTATCC AGTCGAATGA AGTGAGCTCT GGTAGTGCCG ATGTTACCAT TCCAAGGCAG 
CCGTTTCAGA GGTGTGGCAG ATGGTCAGAC CAGAGTGGCT CAGTAAGCAA CGCCTTTGGT 
CGATTTGGAA ACATGGTTGT ACAACAGCAG CCTATAGATT TTATTGATCA GCACTCAGCT 
CTTCAACACA TGGGAGGTAT AAGCTATGGG TGCAGAAATT CAGAGCAGCA TCTACAAGTA 
GCAAAACCAG CCCAAGAGAA GTCCAGGATG AAAACAGGCA TTAAGACTGA GTCTTCCTCA 
ACCTTCCAAC TGGAATCCAA ACACAGCAAC TTTGGTCGAC CAGACTTCTC TCATATCTTT 
TCGGCCCCCA TTCATCAAAA ATGTTCCCAG CATACAGTTG TATCCCAATC TTATCCCCAA 
CAAAATCAAA TTACAAATCA AGATAGCAGC AACCTCTTTT TGCAAAGACC AGGTAACTGC 
AGCTTACTCT TGGCTTACCG GCCTCAGCCA GCTGTCAACA CTTCCTACTC CACTAGGCAG 
GATATTAACA GCCATAATGT ACAATCATGC CATCCACAAA GCTTGAACAA TAGACTAGGC 
ACAAATTGTT CCCCTTCAAA TCAGGTTTCC GTTGTGAAGC TTGATCAAGT TAATCACCTT 
CAAATCAACT CTTGCTCATC AATTCAGCAA CAAACACCTG TGTTTATTGA TAAGTGTTTT 
GACCTAGAAA AGTCCAAGCT CTCTCACGCC ATAGTACAGT CATGTCTAAT GGAATCATTG 
ATGGCAGAGA AAGAAAGAAC TTCCAAAGTT CTTCTGTCAT CTGGGGTTGG TCAGGTGACT 
AGCACAGTAG AAAGAAGCCC AGCTGAAAAA GTTGGTTTAG GAATTTGTTC CATTGGTGAT 
GATAGTTATG ATCAGGGCAG TGTAGTGTCT TCCTCTCAAC TCAGTAATTC TCATGATTCC 
TTTGCATTTC ACAGTGTGAA TCCAGGCACT AACAACATGG CTATTAGTGA CATGAGCTCC 
CTCCTCACCA CCCTCGCAGA AGAGAGTAAA TTTCTGGCAA TCATCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gli3 ( XM_021470119.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325794.1 Danio rerio GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa151663
Clone ID Related Accession (Same CDS sequence)	XM_021470119.1
Accession Version	XM_021470119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCCC AATTCCAGAC CAGTTCTTCA ACAGAGAAGA GAATGGAGAA CGTTGTAGGC 
ACCAATAGTG CACCCACTCG TACAGACATC AGTGAGAAGG CTGTGGCCTC CAGCACTACA 
TCCAATGAGG ATGACAGTTC AGGTTCACCA TATCACCTCG AGAGGCGAAA CACCATTAGT 
GCGCAGACTG ATGTTGTGAA CACATCAGGA AAAGTCAGTG AAGAGCCATC CACCTCTACA 
GACGAACGTC CTCCACTGGT GAAGAAAGAA CTTCAGAGCT CTCTGCACCG CTTGGCAGAC 
CACACCCTGC CCTATCGTGG AACACTGTTT GCCATGGACC CTCGTTCTGG ATATCTGGAC 
CCTCACTATA CTGCACCACA GTTCTTTCCT GCTTTTCATC ATCCAGTACC AATTGATGAT 
AGACACACAC AAGGCCGATA CATTTACGAA CCCTCGCCAG TACCATCACT ACACATGCCT 
CCTGCACTGG CAGCAAGCCC GACATTTTCT GATATCTCCC TCATCCGGAT CTCACCGCAC 
CGGAACCCTT CAATGGGGAC AGACTCACCC TTTAACCCTT CACATCCTTA CATTAACCCC 
TACATGGACT ACATACGCTC CTTGCACAGC AGTCCCTCTT TGTCCATGGT TTCTGCAGCC 
AGAGGCCTTA GTCCTGCAGA TACACATCAC ACTGGTTTAA CCACAGCAGA GTACTACCAC 
CAAATGGCCC TGCTTGCTGG TCACCGCAGT CCCTACGCTG ACCTCATTCC CTCAGTGGGG 
TCCACAACAG GGCCTGCAAG CAGTGCCCTG CATATGGAGT ACCTGCAGGC TATGGAAAGT 
TCGAGATTTC CTAGTCCTAG GCTGATAAAC CGGCCCAGCA GGAAAAGACC CATCCCCAAC 
TCCCCTTCCT TGTCTGACCC CAGTTTTGAC TTACAGGCCA TGATTCGCAC CTCTCCCAAC 
TCTTTAGTCA CTATCCTTAA TAATTCTCGC TCTAGCTCAT CTAACAGTGG ATCTTATGGG 
CACCTATCTG CTGGAACTAT CAGTCCTGCT TTGAGTTTTG CATATCCTCC AACACCTGTA 
GCACTGCAGA TGCATCAGCA GCTAATCAGT CGACAACCAG GCATCGTAGG ATCTGCATTT 
GGACACAGTC CACCACTCAT GCACTCAGCA CCTGCTTTTT CAACCCAGAG ACCTGTTCCT 
GCCATCCAAA CCTCCAGCCT GGCACCAACA GAACGTTCAA TAGTCTCCAA TGACTCACAG 
AGTAAACCCA CAAGTGAGTC GGCTGTGAGC AGCACAGGGG ACCCCATGAA CCATAAAAGG 
TCCAAACTCA AACCTGAAGA AGAACCACCC AGCCCCAGAG CCGTTAGCAT GCAGGAGCAA 
ATGAATGGAA TGACTCTGGT GAAGGAAGAG GGCGATAAAG AGGAGGGCAA GCAGGAGCCA 
GAGGTTGTGT ATGAGACTAA TTGTCACTGG GATGGTTGCT GCAGAGAGTT TGACACCCAA 
GAGCAGCTTG TGCATCACAT AAATAATGAC CATATTCATG GAGAAAAAAA GGAATTTGTG 
TGTCGCTGGG AGGAATGTTC AAGAGAACAG AAACCCTTTA AGGCCCAGTA TATGCTGGTG 
GTGCACATGC GCAGACACAC AGGAGAGAAG CCTCATAAAT GCACGTTTGA AGGCTGTTCA 
AAGGCATATT CCAGATTAGA GAACCTGAAA ACACATTTGC GTTCACACAC AGGAGAGAAG 
CCATATGTGT GTGAACATGA AGGCTGCAAC AAAGCCTTTT CGAATGCATC AGACCGAGCC 
AAACACCAGA ATCGGACACA CTCTAATGAG AAACCCTATG TGTGTAAAAT TCCTGGCTGC 
ACTAAGCGGT ACACTGACCC AAGCTCCTTA AGGAAACATG TTAAAACTGT ACATGGTCCT 
GAAGCCCATG TCACCAAAAA ACAACGTGGG GAGACATACC CACGACCCCC ACCTCAGCCA 
CGAGAACCTG GAGCAACCAC TGACCAAAAG GAGTATACCC ATGCTACCTC AAAACAGGAT 
GAATGTCTGC AAGTCAAGTC AATCAAAACA GAAAAGCCCA TGTGGGTGAT CAGACGTCTC 
AGTCAAGCCC TGGCAGTCAG TCTTCATGCA GCAGTGACCA GTCCATCAAC GGCCCCCATG 
TCAGCCAGGG AGTCCAGCTT ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325794.1
CDS	427..2613
Translation	MEPQFQTSSSTEKRMENVVGTNSAPTRTDISEKAVASSTTSNEDDSSGSPYHLERRNTISAQTDVVNTSGKVSEEPSTSTDERPPLVKKELQSSLHRLADHTLPYRGTLFAMDPRSGYLDPHYTAPQFFPAFHHPVPIDDRHTQGRYIYEPSPVPSLHMPPALAASPTFSDISLIRISPHRNPSMGTDSPFNPSHPYINPYMDYIRSLHSSPSLSMVSAARGLSPADTHHTGLTTAEYYHQMALLAGHRSPYADLIPSVGSTTGPASSALHMEYLQAMESSRFPSPRLINRPSRKRPIPNSPSLSDPSFDLQAMIRTSPNSLVTILNNSRSSSSNSGSYGHLSAGTISPALSFAYPPTPVALQMHQQLISRQPGIVGSAFGHSPPLMHSAPAFSTQRPVPAIQTSSLAPTERSIVSNDSQSKPTSESAVSSTGDPMNHKRSKLKPEEEPPSPRAVSMQEQMNGMTLVKEEGDKEEGKQEPEVVYETNCHWDGCCREFDTQEQLVHHINNDHIHGEKKEFVCRWEECSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGETYPRPPPQPREPGATTDQKEYTHATSKQDECLQVKSIKTEKPMWVIRRLSQALAVSLHAAVTSPSTAPMSARESSLL

Target ORF information:

RefSeq Version	XM_021470119.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLI family zinc finger 3 (gli3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470119.1

ORF Insert Sequence:

ATGGAACCCC AATTCCAGAC CAGTTCTTCA ACAGAGAAGA GAATGGAGAA CGTTGTAGGC 
ACCAATAGTG CACCCACTCG TACAGACATC AGTGAGAAGG CTGTGGCCTC CAGCACTACA 
TCCAATGAGG ATGACAGTTC AGGTTCACCA TATCACCTCG AGAGGCGAAA CACCATTAGT 
GCGCAGACTG ATGTTGTGAA CACATCAGGA AAAGTCAGTG AAGAGCCATC CACCTCTACA 
GACGAACGTC CTCCACTGGT GAAGAAAGAA CTTCAGAGCT CTCTGCACCG CTTGGCAGAC 
CACACCCTGC CCTATCGTGG AACACTGTTT GCCATGGACC CTCGTTCTGG ATATCTGGAC 
CCTCACTATA CTGCACCACA GTTCTTTCCT GCTTTTCATC ATCCAGTACC AATTGATGAT 
AGACACACAC AAGGCCGATA CATTTACGAA CCCTCGCCAG TACCATCACT ACACATGCCT 
CCTGCACTGG CAGCAAGCCC GACATTTTCT GATATCTCCC TCATCCGGAT CTCACCGCAC 
CGGAACCCTT CAATGGGGAC AGACTCACCC TTTAACCCTT CACATCCTTA CATTAACCCC 
TACATGGACT ACATACGCTC CTTGCACAGC AGTCCCTCTT TGTCCATGGT TTCTGCAGCC 
AGAGGCCTTA GTCCTGCAGA TACACATCAC ACTGGTTTAA CCACAGCAGA GTACTACCAC 
CAAATGGCCC TGCTTGCTGG TCACCGCAGT CCCTACGCTG ACCTCATTCC CTCAGTGGGG 
TCCACAACAG GGCCTGCAAG CAGTGCCCTG CATATGGAGT ACCTGCAGGC TATGGAAAGT 
TCGAGATTTC CTAGTCCTAG GCTGATAAAC CGGCCCAGCA GGAAAAGACC CATCCCCAAC 
TCCCCTTCCT TGTCTGACCC CAGTTTTGAC TTACAGGCCA TGATTCGCAC CTCTCCCAAC 
TCTTTAGTCA CTATCCTTAA TAATTCTCGC TCTAGCTCAT CTAACAGTGG ATCTTATGGG 
CACCTATCTG CTGGAACTAT CAGTCCTGCT TTGAGTTTTG CATATCCTCC AACACCTGTA 
GCACTGCAGA TGCATCAGCA GCTAATCAGT CGACAACCAG GCATCGTAGG ATCTGCATTT 
GGACACAGTC CACCACTCAT GCACTCAGCA CCTGCTTTTT CAACCCAGAG ACCTGTTCCT 
GCCATCCAAA CCTCCAGCCT GGCACCAACA GAACGTTCAA TAGTCTCCAA TGACTCACAG 
AGTAAACCCA CAAGTGAGTC GGCTGTGAGC AGCACAGGGG ACCCCATGAA CCATAAAAGG 
TCCAAACTCA AACCTGAAGA AGAACCACCC AGCCCCAGAG CCGTTAGCAT GCAGGAGCAA 
ATGAATGGAA TGACTCTGGT GAAGGAAGAG GGCGATAAAG AGGAGGGCAA GCAGGAGCCA 
GAGGTTGTGT ATGAGACTAA TTGTCACTGG GATGGTTGCT GCAGAGAGTT TGACACCCAA 
GAGCAGCTTG TGCATCACAT AAATAATGAC CATATTCATG GAGAAAAAAA GGAATTTGTG 
TGTCGCTGGG AGGAATGTTC AAGAGAACAG AAACCCTTTA AGGCCCAGTA TATGCTGGTG 
GTGCACATGC GCAGACACAC AGGAGAGAAG CCTCATAAAT GCACGTTTGA AGGCTGTTCA 
AAGGCATATT CCAGATTAGA GAACCTGAAA ACACATTTGC GTTCACACAC AGGAGAGAAG 
CCATATGTGT GTGAACATGA AGGCTGCAAC AAAGCCTTTT CGAATGCATC AGACCGAGCC 
AAACACCAGA ATCGGACACA CTCTAATGAG AAACCCTATG TGTGTAAAAT TCCTGGCTGC 
ACTAAGCGGT ACACTGACCC AAGCTCCTTA AGGAAACATG TTAAAACTGT ACATGGTCCT 
GAAGCCCATG TCACCAAAAA ACAACGTGGG GAGACATACC CACGACCCCC ACCTCAGCCA 
CGAGAACCTG GAGCAACCAC TGACCAAAAG GAGTATACCC ATGCTACCTC AAAACAGGAT 
GAATGTCTGC AAGTCAAGTC AATCAAAACA GAAAAGCCCA TGTGGGTGAT CAGACGTCTC 
AGTCAAGCCC TGGCAGTCAG TCTTCATGCA GCAGTGACCA GTCCATCAAC GGCCCCCATG 
TCAGCCAGGG AGTCCAGCTT ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gli3 ( NM_205728.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991291.1 Danio rerio GLI family zinc finger 3 (gli3), mRNA.

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CloneID	ODa03571
Clone ID Related Accession (Same CDS sequence)	NM_205728.1
Accession Version	NM_205728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	transcriptional activator GLI3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY377429.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY377429.1, BC163726.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAACCCC AATTCCAGAC CAGTTCTTCA ACAGAGAAGA GAATGGAGAA CGTTGTAGGC 
ACCAATAGTG CACCCACTCG TACAGACATC AGTGAGAAGG CTGTGGCCTC CAGCACTACA 
TCCAATGAGG ATGACAGTTC AGGTTCACCA TATCACCTCG AGAGGCGAAA CACCATTAGT 
GCGCAGACTG ATGTTGTGAA CACATCAGGA AAAGTCAGTG AAGAGCCATC CACTTCTACA 
GACGAACGTC TTCCACTGGT GAAGAAAGAA CTTCAGAGCT TTCTGCACCG CTTGGCAGAC 
CACACCTTGC CTTATCGTGG AACACTGTTT GCCATGGACC TTCGTTCTGG ATATCTGGAC 
CCTCACTATA CTGCACCACA GTTCTTTCGT GCTTTTCATC ATCCAGTACC AATTGATGAT 
AGACACACAC AAGGCCGATA CATTTACGAA CCTTCGCCAG TACCATCACT ACACATGCCT 
CCTGCACTGG CAGCAAGCCC GACATTTTCT GATATCTCCC TCATCCGGAT CTCACCGCAC 
CGGAACCCTT CAATGGGGAC AGACTCACCC TTTAACCCTT CACATCCTTA CATTAACCCC 
TACATGGACT ACATACGCTC CTTGCACAGC AGTCCCTCTT TGTCCATGGT TTCTGCAGCC 
AGAGGCCTTA GTCCTGCAGA TACACATCAC ACTGGTTTAA CCACAGCAGA GTATTACCAC 
CAAATGGCCC TGCTTGTTGG TCACCGCAGT CCCTACGCTG ACCTCATTCC CTCAGTGGGG 
TCCACAACAG GGCCTGCAAG CAGTGCCCTG CATATGGAGT ACCTGCAGGC TATGGAAAGT 
TCGAGATTTC CTAGTCCTAG GCTGATAAAC CGGCCCAGCA GGAAAAGACC CATCCCCAAC 
TCCCCTTCCT TGTCTGACCC CAGTTTTGAC TTACAGGCCA TGATTCGCAC CTCTCCCAAC 
TCTTTAGTCA CTATCCTTAA TAATTCTCGC TCTAGCTCAT CTAACAGTGG ATCTTATGGG 
CACCTATCTG CTGGAACTAT CAGTCCTGCT TTGAGTTTTG CATATCCTCC AACACCTGTA 
GCACTGCAGA TGCATCAGCA GCTAATCAGT CGACAACCAG GCATCGTAGG ATCTGCATTT 
GGACACAGTC CACCACTCAT GCACTCAGCA CTTGCTTTTT CAACCCAGAG ACCTGTTCCT 
GCCATCCAAA CCTCCAGCCT GGCACCAACA GAACGTTCAA TAGTCTCCAA TGACTCACAG 
AGTAAACCCA CAAGTGAGTC GGCTGTGAGC AGCACAGGGG ACCCCATGAA CCATAAAAGG 
TCCAAACTCA AACCTGAAGA AGAACCACCC AGCCCCAGAG CCGTTAGCAT GCAGGAGCAA 
ATGAATGGAA TGACTCTGGT GAAGGAAGAG GGCGATAAAG AGGAGGGCAA GCAGGAGCCA 
GAGGTTGTGT ATGAGACTAA TTGTCACTGG GATGGTTGCT GCAGAGAGTT TGACACCCAA 
GAGCAGCTTG TGCATCACAT AAATAATGAC CATATTCATG GAGAAAAAAA GGAATTTGTG 
TGTCGCTGGG AGGAATGTTC AAGAGAACAG AAACCCTTTA AGGCCCAGTA TATGCTGGTG 
GTGCACATGC GCAGACACAC AGGAGAGAAG CCTCATAAAT GCACGTTTGA AGGCTGTTCA 
AAGGCATATT CCAGATTAGA GAACCTGAAA ACACATTTGC GTTCACACAC AGGAGAGAAG 
CCATATGTGT GTGAACATGA AGGCTGCAAC AAAGCCTTTT CGAATGCATC AGACCGAGCC 
AAACACCAGA ATCGGACACA CTCTAATGAG AAACCCTATG TGTGTAAAAT TCCTGGTTGC 
ACTAAACGGT ACACTGACCC AAGCTCCTTA AGGAAACATG TTAAAACTGT ACATGGTCCT 
GAAGCCCATG TCACCAAAAA ACAACGTGGG GAGACATACC CACGACCCCC ACCTCAGCCA 
CGAGAACCTG GAGCAACCAC TGACCAAAAG GAGTATACCC ATGCTACCTC AAAACAGGAT 
GAATGTCTGC AAGTCAAGTC AATCAAAACA GAAAAGCCCA TGACGTCTCA GTCAAGCCCT 
GGCAGTCAGT CTTCATGCAG CAGTGACCAG TCCATCAACG GCCCCCATGT CAGCCAGGGA 
GTCCAGCTTA CTGTGAACAG TATTGGGCTT GCGGGGGAGG TGGCAATGCT TGAGGACATG 
GAAAATGAAG AGGAGGATGA GGAGAACAGA GAAGGAGAGA AGGAGGATGC CCCCATTATG 
GACTCTACAG TATCCACAGC AACATCCTCT GCGGCAACAA TAGTTTTGCA GACTCATCGA 
AGTGTTGGAC GGCCCCTACG CTGGATGGAG CATGTTAAGA TGGAGAGGTT AAGGCAGGTG 
AATGGAGCTG TGCCTAGGCT AGGATTCCTG CCTCCAACAC CGCCCCGTAA AGGCCAGGCC 
ATGCCTGCCC TCACAGGAAA GGGTCAATGC CTTGGGAGGA TGACTTCTGT GAGGGTGCCT 
CCATGCCCTG AGCCGTCCAA CACAGAGCTG ACAATTCTGA GTTTGCTGCA TGAACGACGG 
GACAGTAGTG GCAGCAACAC TAGCTCAGCT TACCTGAGCA GCAGCCGTCG CTCATCTGGC 
ATCTCCCCCT GCTTTTCCAG TCGCTGTTCT AGCCAGGCTT CACTGTCAGA GTACAGCCCC 
CATCGACATA TCCATAATCT CAGTGCAACT GATTCTTATG ACCCTATTTC CACTGATGCC 
TCTCGACGCT CCAGTGAAGC CAGCCAGTAT GATGGAGGAA GTTCAAGTGC AGTGGTGTTT 
GGTGGAAGTG GAGTTTTCAA GGGCAGTGGT ATGGGATCAA GAGGGGATCT GAACCTCACT 
CCAGCTCAAC ACTATAGCCT AAAGGCCAAG TATGCAGCAG CAACAGGTGG ACCTCCTCCC 
ACACCCTTGC CCAGTATGGG GCATATAAAC CTAAAGACTC ACTTTGCTTT AAAGGAAGAC 
AGAAAGGACA CTGGCCAGCT TACACTACCA CCACTGGTCA GGTCACGTCG CTGTAGTGAT 
GGTGCACACA ACTCGCATGA CAGTCAAGCC GTGTATCAAC GAAAGGGAAC ATACTTTGGT 
GGTGGTCCAG GGAAAAATGA TCGAAGAGCT AGTGATCCTA CACATCACAA ACAAAATCTT 
AGTTCTTATG GCTTGCCACA AGTACGACAT TTCAGTAGTT TAAACAATAT GACTTCATCA 
CCCAATCCTG CTAGTTATGG TTACTCAAAC AGAGAAGATC ACAGTCTGAA CCTACAGAAT 
TCAACCTGTC ATGTAGATAG CTTTCAGAGT GGTCTGCACT CCCCTTGTCC TCCTAGCATC 
ATGGAGCAAT CAGCACTTGA GAACATTTTC ATGAACCAGG ATGGTCTTTT GTTACGTGTC 
AATGAAAACA TCCAGTCTGA TTTCATGCAG TATCTCCATT CCCAAGATCA GGAATCAATA 
ATTATCCAAG ACAACCATAG GCCTATTTTC AATCCTGCAG ATGACCAAAA CTTCCTCAGT 
CCACAGCAGC AGCAAGAGGG GGTAGAGCAA GAAGACACTC GTATAGATCT GATGCCTATC 
CAGTCGAATG AAGTGAGCTC TGCTAGTGCT GATGTTACTA TTCCAAGGCA GCCGTTTCAG 
AGGTGTGGCA GATGGTCAGA CCAGAGTGGC TCAGTAAGCA ACGCCTTTGG TCGATTTGGA 
AACATGGTTG TACAACAGCA GCCTATAGAT TTTATTGATC AGCACTCAGC TCTTCAACAC 
ATGGGAGGTA TAAGCTATGG GTGCAGAAAT TCAGAGCAGC ATCTACAAGT AGCAAAACCA 
GCCCAAGAGA AGTCCAGGAT GAAAACAGGC ATTAAGACTG AGTCTTCCTC AACCTTCCAA 
CTGGAATCCA AACACAGCAA CTTTGGTCGA CCAGACTTCT CTCATATCTT TTCGGCCCCC 
ATTCATCAAA AATGTTCCCA GCATACAGTT GTATCCCAAT CTTATCCCCA ACAAAATCAA 
ATTACAAATC AAGATAGCAG CAACCTCTTT TTGCAAAGAC CAGGTAACTG CAGCTTACTC 
TTGGCTTACC GGCCTCAGCC AGCTGTCAAC ACTTCTTACT CCACTAGGCA GGATATTAAC 
AGCCATAATG TACAATCATG CCATCCACAA AGCTTGAACA ATAGACTAGG CACAAGTTGT 
TCCCTTTCAA ATCAGGTTTC CGTTGTGAAG CTTGATCAAG TTAATCACCT TCAAATCAAC 
TCTTGCTCAA CAATTCAGCA ACAAAAGCCT GTGTTTATTG ATAAGTGTTT TGACCCAGAA 
GAGTCCAAGC TCTCTCACGC CATAGTACAG TCATGTCTAA TGGAATCATT GATGGCAGAG 
AAAGAAAGAA CTTCCAAAGT TCTTCTGTCA TCTGGGGTTG GTCAGGTGAC TAGCAAAGTA 
GAAAGAAGCC CAGCTGAAAA AGTTGGTTTA GGAATTTGTT CCATTGGTGG TGATAGTTAT 
GATCAGGGCA GTGTAGTGTC TTCCTCTCGA CTCAGTAATT CTCATGATTC CTTTGCATTT 
CACAGTGTGG ATCCAGGCAC TAACAACATG GCTATTAGTG ACATGAACTC CCTCCTCACC 
ACCCTCGCAG AAGAGAGTAA ATTTCTGGCA ATCATCCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991291.1
CDS	386..5047
Translation	MEPQFQTSSSTEKRMENVVGTNSAPTRTDISEKAVASSTTSNEDDSSGSPYHLERRNTISAQTDVVNTSGKVSEEPSTSTDERLPLVKKELQSFLHRLADHTLPYRGTLFAMDLRSGYLDPHYTAPQFFRAFHHPVPIDDRHTQGRYIYEPSPVPSLHMPPALAASPTFSDISLIRISPHRNPSMGTDSPFNPSHPYINPYMDYIRSLHSSPSLSMVSAARGLSPADTHHTGLTTAEYYHQMALLVGHRSPYADLIPSVGSTTGPASSALHMEYLQAMESSRFPSPRLINRPSRKRPIPNSPSLSDPSFDLQAMIRTSPNSLVTILNNSRSSSSNSGSYGHLSAGTISPALSFAYPPTPVALQMHQQLISRQPGIVGSAFGHSPPLMHSALAFSTQRPVPAIQTSSLAPTERSIVSNDSQSKPTSESAVSSTGDPMNHKRSKLKPEEEPPSPRAVSMQEQMNGMTLVKEEGDKEEGKQEPEVVYETNCHWDGCCREFDTQEQLVHHINNDHIHGEKKEFVCRWEECSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGETYPRPPPQPREPGATTDQKEYTHATSKQDECLQVKSIKTEKPMTSQSSPGSQSSCSSDQSINGPHVSQGVQLTVNSIGLAGEVAMLEDMENEEEDEENREGEKEDAPIMDSTVSTATSSAATIVLQTHRSVGRPLRWMEHVKMERLRQVNGAVPRLGFLPPTPPRKGQAMPALTGKGQCLGRMTSVRVPPCPEPSNTELTILSLLHERRDSSGSNTSSAYLSSSRRSSGISPCFSSRCSSQASLSEYSPHRHIHNLSATDSYDPISTDASRRSSEASQYDGGSSSAVVFGGSGVFKGSGMGSRGDLNLTPAQHYSLKAKYAAATGGPPPTPLPSMGHINLKTHFALKEDRKDTGQLTLPPLVRSRRCSDGAHNSHDSQAVYQRKGTYFGGGPGKNDRRASDPTHHKQNLSSYGLPQVRHFSSLNNMTSSPNPASYGYSNREDHSLNLQNSTCHVDSFQSGLHSPCPPSIMEQSALENIFMNQDGLLLRVNENIQSDFMQYLHSQDQESIIIQDNHRPIFNPADDQNFLSPQQQQEGVEQEDTRIDLMPIQSNEVSSASADVTIPRQPFQRCGRWSDQSGSVSNAFGRFGNMVVQQQPIDFIDQHSALQHMGGISYGCRNSEQHLQVAKPAQEKSRMKTGIKTESSSTFQLESKHSNFGRPDFSHIFSAPIHQKCSQHTVVSQSYPQQNQITNQDSSNLFLQRPGNCSLLLAYRPQPAVNTSYSTRQDINSHNVQSCHPQSLNNRLGTSCSLSNQVSVVKLDQVNHLQINSCSTIQQQKPVFIDKCFDPEESKLSHAIVQSCLMESLMAEKERTSKVLLSSGVGQVTSKVERSPAEKVGLGICSIGGDSYDQGSVVSSSRLSNSHDSFAFHSVDPGTNNMAISDMNSLLTTLAEESKFLAIIQ

Target ORF information:

RefSeq Version	NM_205728.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLI family zinc finger 3 (gli3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205728.1

ORF Insert Sequence:

ATGGAACCCC AATTCCAGAC CAGTTCTTCA ACAGAGAAGA GAATGGAGAA CGTTGTAGGC 
ACCAATAGTG CACCCACTCG TACAGACATC AGTGAGAAGG CTGTGGCCTC CAGCACTACA 
TCCAATGAGG ATGACAGTTC AGGTTCACCA TATCACCTCG AGAGGCGAAA CACCATTAGT 
GCGCAGACTG ATGTTGTGAA CACATCAGGA AAAGTCAGTG AAGAGCCATC CACTTCTACA 
GACGAACGTC TTCCACTGGT GAAGAAAGAA CTTCAGAGCT TTCTGCACCG CTTGGCAGAC 
CACACCTTGC CTTATCGTGG AACACTGTTT GCCATGGACC TTCGTTCTGG ATATCTGGAC 
CCTCACTATA CTGCACCACA GTTCTTTCGT GCTTTTCATC ATCCAGTACC AATTGATGAT 
AGACACACAC AAGGCCGATA CATTTACGAA CCTTCGCCAG TACCATCACT ACACATGCCT 
CCTGCACTGG CAGCAAGCCC GACATTTTCT GATATCTCCC TCATCCGGAT CTCACCGCAC 
CGGAACCCTT CAATGGGGAC AGACTCACCC TTTAACCCTT CACATCCTTA CATTAACCCC 
TACATGGACT ACATACGCTC CTTGCACAGC AGTCCCTCTT TGTCCATGGT TTCTGCAGCC 
AGAGGCCTTA GTCCTGCAGA TACACATCAC ACTGGTTTAA CCACAGCAGA GTATTACCAC 
CAAATGGCCC TGCTTGTTGG TCACCGCAGT CCCTACGCTG ACCTCATTCC CTCAGTGGGG 
TCCACAACAG GGCCTGCAAG CAGTGCCCTG CATATGGAGT ACCTGCAGGC TATGGAAAGT 
TCGAGATTTC CTAGTCCTAG GCTGATAAAC CGGCCCAGCA GGAAAAGACC CATCCCCAAC 
TCCCCTTCCT TGTCTGACCC CAGTTTTGAC TTACAGGCCA TGATTCGCAC CTCTCCCAAC 
TCTTTAGTCA CTATCCTTAA TAATTCTCGC TCTAGCTCAT CTAACAGTGG ATCTTATGGG 
CACCTATCTG CTGGAACTAT CAGTCCTGCT TTGAGTTTTG CATATCCTCC AACACCTGTA 
GCACTGCAGA TGCATCAGCA GCTAATCAGT CGACAACCAG GCATCGTAGG ATCTGCATTT 
GGACACAGTC CACCACTCAT GCACTCAGCA CTTGCTTTTT CAACCCAGAG ACCTGTTCCT 
GCCATCCAAA CCTCCAGCCT GGCACCAACA GAACGTTCAA TAGTCTCCAA TGACTCACAG 
AGTAAACCCA CAAGTGAGTC GGCTGTGAGC AGCACAGGGG ACCCCATGAA CCATAAAAGG 
TCCAAACTCA AACCTGAAGA AGAACCACCC AGCCCCAGAG CCGTTAGCAT GCAGGAGCAA 
ATGAATGGAA TGACTCTGGT GAAGGAAGAG GGCGATAAAG AGGAGGGCAA GCAGGAGCCA 
GAGGTTGTGT ATGAGACTAA TTGTCACTGG GATGGTTGCT GCAGAGAGTT TGACACCCAA 
GAGCAGCTTG TGCATCACAT AAATAATGAC CATATTCATG GAGAAAAAAA GGAATTTGTG 
TGTCGCTGGG AGGAATGTTC AAGAGAACAG AAACCCTTTA AGGCCCAGTA TATGCTGGTG 
GTGCACATGC GCAGACACAC AGGAGAGAAG CCTCATAAAT GCACGTTTGA AGGCTGTTCA 
AAGGCATATT CCAGATTAGA GAACCTGAAA ACACATTTGC GTTCACACAC AGGAGAGAAG 
CCATATGTGT GTGAACATGA AGGCTGCAAC AAAGCCTTTT CGAATGCATC AGACCGAGCC 
AAACACCAGA ATCGGACACA CTCTAATGAG AAACCCTATG TGTGTAAAAT TCCTGGTTGC 
ACTAAACGGT ACACTGACCC AAGCTCCTTA AGGAAACATG TTAAAACTGT ACATGGTCCT 
GAAGCCCATG TCACCAAAAA ACAACGTGGG GAGACATACC CACGACCCCC ACCTCAGCCA 
CGAGAACCTG GAGCAACCAC TGACCAAAAG GAGTATACCC ATGCTACCTC AAAACAGGAT 
GAATGTCTGC AAGTCAAGTC AATCAAAACA GAAAAGCCCA TGACGTCTCA GTCAAGCCCT 
GGCAGTCAGT CTTCATGCAG CAGTGACCAG TCCATCAACG GCCCCCATGT CAGCCAGGGA 
GTCCAGCTTA CTGTGAACAG TATTGGGCTT GCGGGGGAGG TGGCAATGCT TGAGGACATG 
GAAAATGAAG AGGAGGATGA GGAGAACAGA GAAGGAGAGA AGGAGGATGC CCCCATTATG 
GACTCTACAG TATCCACAGC AACATCCTCT GCGGCAACAA TAGTTTTGCA GACTCATCGA 
AGTGTTGGAC GGCCCCTACG CTGGATGGAG CATGTTAAGA TGGAGAGGTT AAGGCAGGTG 
AATGGAGCTG TGCCTAGGCT AGGATTCCTG CCTCCAACAC CGCCCCGTAA AGGCCAGGCC 
ATGCCTGCCC TCACAGGAAA GGGTCAATGC CTTGGGAGGA TGACTTCTGT GAGGGTGCCT 
CCATGCCCTG AGCCGTCCAA CACAGAGCTG ACAATTCTGA GTTTGCTGCA TGAACGACGG 
GACAGTAGTG GCAGCAACAC TAGCTCAGCT TACCTGAGCA GCAGCCGTCG CTCATCTGGC 
ATCTCCCCCT GCTTTTCCAG TCGCTGTTCT AGCCAGGCTT CACTGTCAGA GTACAGCCCC 
CATCGACATA TCCATAATCT CAGTGCAACT GATTCTTATG ACCCTATTTC CACTGATGCC 
TCTCGACGCT CCAGTGAAGC CAGCCAGTAT GATGGAGGAA GTTCAAGTGC AGTGGTGTTT 
GGTGGAAGTG GAGTTTTCAA GGGCAGTGGT ATGGGATCAA GAGGGGATCT GAACCTCACT 
CCAGCTCAAC ACTATAGCCT AAAGGCCAAG TATGCAGCAG CAACAGGTGG ACCTCCTCCC 
ACACCCTTGC CCAGTATGGG GCATATAAAC CTAAAGACTC ACTTTGCTTT AAAGGAAGAC 
AGAAAGGACA CTGGCCAGCT TACACTACCA CCACTGGTCA GGTCACGTCG CTGTAGTGAT 
GGTGCACACA ACTCGCATGA CAGTCAAGCC GTGTATCAAC GAAAGGGAAC ATACTTTGGT 
GGTGGTCCAG GGAAAAATGA TCGAAGAGCT AGTGATCCTA CACATCACAA ACAAAATCTT 
AGTTCTTATG GCTTGCCACA AGTACGACAT TTCAGTAGTT TAAACAATAT GACTTCATCA 
CCCAATCCTG CTAGTTATGG TTACTCAAAC AGAGAAGATC ACAGTCTGAA CCTACAGAAT 
TCAACCTGTC ATGTAGATAG CTTTCAGAGT GGTCTGCACT CCCCTTGTCC TCCTAGCATC 
ATGGAGCAAT CAGCACTTGA GAACATTTTC ATGAACCAGG ATGGTCTTTT GTTACGTGTC 
AATGAAAACA TCCAGTCTGA TTTCATGCAG TATCTCCATT CCCAAGATCA GGAATCAATA 
ATTATCCAAG ACAACCATAG GCCTATTTTC AATCCTGCAG ATGACCAAAA CTTCCTCAGT 
CCACAGCAGC AGCAAGAGGG GGTAGAGCAA GAAGACACTC GTATAGATCT GATGCCTATC 
CAGTCGAATG AAGTGAGCTC TGCTAGTGCT GATGTTACTA TTCCAAGGCA GCCGTTTCAG 
AGGTGTGGCA GATGGTCAGA CCAGAGTGGC TCAGTAAGCA ACGCCTTTGG TCGATTTGGA 
AACATGGTTG TACAACAGCA GCCTATAGAT TTTATTGATC AGCACTCAGC TCTTCAACAC 
ATGGGAGGTA TAAGCTATGG GTGCAGAAAT TCAGAGCAGC ATCTACAAGT AGCAAAACCA 
GCCCAAGAGA AGTCCAGGAT GAAAACAGGC ATTAAGACTG AGTCTTCCTC AACCTTCCAA 
CTGGAATCCA AACACAGCAA CTTTGGTCGA CCAGACTTCT CTCATATCTT TTCGGCCCCC 
ATTCATCAAA AATGTTCCCA GCATACAGTT GTATCCCAAT CTTATCCCCA ACAAAATCAA 
ATTACAAATC AAGATAGCAG CAACCTCTTT TTGCAAAGAC CAGGTAACTG CAGCTTACTC 
TTGGCTTACC GGCCTCAGCC AGCTGTCAAC ACTTCTTACT CCACTAGGCA GGATATTAAC 
AGCCATAATG TACAATCATG CCATCCACAA AGCTTGAACA ATAGACTAGG CACAAGTTGT 
TCCCTTTCAA ATCAGGTTTC CGTTGTGAAG CTTGATCAAG TTAATCACCT TCAAATCAAC 
TCTTGCTCAA CAATTCAGCA ACAAAAGCCT GTGTTTATTG ATAAGTGTTT TGACCCAGAA 
GAGTCCAAGC TCTCTCACGC CATAGTACAG TCATGTCTAA TGGAATCATT GATGGCAGAG 
AAAGAAAGAA CTTCCAAAGT TCTTCTGTCA TCTGGGGTTG GTCAGGTGAC TAGCAAAGTA 
GAAAGAAGCC CAGCTGAAAA AGTTGGTTTA GGAATTTGTT CCATTGGTGG TGATAGTTAT 
GATCAGGGCA GTGTAGTGTC TTCCTCTCGA CTCAGTAATT CTCATGATTC CTTTGCATTT 
CACAGTGTGG ATCCAGGCAC TAACAACATG GCTATTAGTG ACATGAACTC CCTCCTCACC 
ACCCTCGCAG AAGAGAGTAA ATTTCTGGCA ATCATCCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

let-7 MicroRNA-Mediated Regulation of Shh Signaling and the Gene Regulatory Network Is Essential for Retina Regeneration.
Cell reports23(5)1409-1423(2018 05)
Kaur S,Gupta S,Chaudhary M,Khursheed MA,Mitra S,Kurup AJ,Ramachandran R

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Molecular mechanisms underlying the exceptional adaptations of batoid fins.
Proceedings of the National Academy of Sciences of the United States of America112(52)15940-5(2015 Dec)
Nakamura T,Klomp J,Pieretti J,Schneider I,Gehrke AR,Shubin NH

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Sox4 regulates choroid fissure closure by limiting Hedgehog signaling during ocular morphogenesis.
Developmental biology399(1)139-153(2015 Mar)
Wen W,Pillai-Kastoori L,Wilson SG,Morris AC

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gli3 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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