GLI3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLI3
Entrez Gene ID	785371
Full Name	GLI family zinc finger 3
General protein information	Preferred Names GLI family zinc finger 3 Names zinc finger protein GLI3 transcriptional activator GLI3 GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) GLI-kruppel family member 3-like protein transcriptional activator GLI3-like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015463085.1	XP_015318571.1	transcriptional activator GLI3 isoform X1
XM_001253425.4	XP_001253426.2	transcriptional activator GLI3 isoform X2
XM_005205661.3	XP_005205718.1	transcriptional activator GLI3 isoform X2
XM_005205661.4	XP_005205718.1	transcriptional activator GLI3 isoform X2
XM_005205659.3	XP_005205716.1	transcriptional activator GLI3 isoform X1
XM_005205659.4	XP_005205716.1	transcriptional activator GLI3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
Macaca mulatta (Rhesus monkey)	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Gene Ontology

The following GLI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa03954	XM_005205661.4 Latest version!	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa03954	XM_001253425.4 Latest version!	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa18737	XM_005205659.4 Latest version!	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa18737	XM_015463085.1 Latest version!	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OBa03954	XM_005205661.3	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa18737	XM_005205659.3	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLI3 ( XM_001253425.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_001253426.2 Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa03954
Clone ID Related Accession (Same CDS sequence)	XM_001253425.4 , XM_005205661.3 , XM_005205661.4
Accession Version	XM_001253425.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001253425.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG 
CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG 
CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC 
AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT 
TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT 
CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT 
CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC 
ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC 
CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG 
CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA 
GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT 
CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC 
TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG 
CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG 
ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA 
GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC 
TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA 
GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT 
CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC 
ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC 
ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC 
AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC 
CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG 
TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC 
AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC 
AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC 
CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG 
GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC 
TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC 
ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC 
AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC 
GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC 
GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC 
CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC 
GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA 
GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA 
GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG 
GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG 
GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG 
AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT 
GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC 
AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT 
ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG 
CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC 
CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG 
AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC 
TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG 
CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC 
GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC 
AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC 
GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT 
CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG 
AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA 
AGCAAATTCC TTGCGGTTAT GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001253426.2
CDS	311..4897
Translation	MQPQIAPGLSKISEEPSTSSDERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDLVHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDVHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGGVGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMLPRLNPILPPKAPAVSPLIGNGTQPSTSCSLGGPMPLLPNRSDLSGVDITMLNMLNRRDSSTSTVSSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDGREPAGTLPPVHPPRRCSDGGAHGYSRRPLLPQDALGNGVRRASDPVRTVSESLSLPRVQRFNSLNSFNPPVLPPSLEKRNLVLQNYTRPDGGQARHFHSSPCPPSITENVTLESLTMDADVSLNDEDLLPDDVVQYLNSQNPAGYEQHFPGTFSDDSKVPHGLGSGPGDFDPLGLPDSHASHTGSQQYRSLEQPCPDASKSDLPIQWNEVSSGSADLSSSKLKCGQRSMGQQARAFGSYSHMGVHPQNPLRSGAGPAGGYPTLGEHASSFGGPEHLVSHGGGAGTNGNAFHEPPYKAQQYGNCLNRQPGAPTLLDGACGPGIQAAKLKSITMQGSGSHLDFGLSVVPNESTGSTVNGIPTPDPTGQGYLAQQLLGDSTHTQGAGRSGQNLLGQASATSHIYQGPESSLPGPPSISVQPPNVAAVRGYQPCVGYGGSRRQAGPRGGLALQPGQFSDPSQTCRVNGIKMEMPGQPHQLCSNVQSYSGQFYDQTVGFGQQDMKTGSFSMSEANCLLQGAGAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRAALPFPPLSLSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_001253425.4
Organism	Bos taurus(cattle)
Definition	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001253425.4

ORF Insert Sequence:

ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG 
CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG 
CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC 
AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT 
TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT 
CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT 
CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC 
ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC 
CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG 
CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA 
GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT 
CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC 
TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG 
CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG 
ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA 
GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC 
TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA 
GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT 
CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC 
ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC 
ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC 
AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC 
CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG 
TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC 
AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC 
AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC 
CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG 
GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC 
TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC 
ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC 
AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC 
GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC 
GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC 
CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC 
GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA 
GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA 
GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG 
GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG 
GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG 
AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT 
GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC 
AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT 
ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG 
CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC 
CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG 
AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC 
TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG 
CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC 
GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC 
AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC 
GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT 
CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG 
AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA 
AGCAAATTCC TTGCGGTTAT GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_005205661.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205718.1 Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa03954
Clone ID Related Accession (Same CDS sequence)	XM_001253425.4 , XM_005205661.3 , XM_005205661.4
Accession Version	XM_005205661.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG 
CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG 
CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC 
AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT 
TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT 
CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT 
CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC 
ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC 
CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG 
CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA 
GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT 
CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC 
TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG 
CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG 
ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA 
GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC 
TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA 
GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT 
CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC 
ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC 
ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC 
AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC 
CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG 
TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC 
AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC 
AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC 
CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG 
GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC 
TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC 
ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC 
AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC 
GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC 
GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC 
CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC 
GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA 
GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA 
GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG 
GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG 
GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG 
AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT 
GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC 
AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT 
ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG 
CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC 
CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG 
AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC 
TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG 
CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC 
GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC 
AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC 
GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT 
CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG 
AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA 
AGCAAATTCC TTGCGGTTAT GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205718.1
CDS	311..4897
Translation	MQPQIAPGLSKISEEPSTSSDERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDLVHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDVHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGGVGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMLPRLNPILPPKAPAVSPLIGNGTQPSTSCSLGGPMPLLPNRSDLSGVDITMLNMLNRRDSSTSTVSSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDGREPAGTLPPVHPPRRCSDGGAHGYSRRPLLPQDALGNGVRRASDPVRTVSESLSLPRVQRFNSLNSFNPPVLPPSLEKRNLVLQNYTRPDGGQARHFHSSPCPPSITENVTLESLTMDADVSLNDEDLLPDDVVQYLNSQNPAGYEQHFPGTFSDDSKVPHGLGSGPGDFDPLGLPDSHASHTGSQQYRSLEQPCPDASKSDLPIQWNEVSSGSADLSSSKLKCGQRSMGQQARAFGSYSHMGVHPQNPLRSGAGPAGGYPTLGEHASSFGGPEHLVSHGGGAGTNGNAFHEPPYKAQQYGNCLNRQPGAPTLLDGACGPGIQAAKLKSITMQGSGSHLDFGLSVVPNESTGSTVNGIPTPDPTGQGYLAQQLLGDSTHTQGAGRSGQNLLGQASATSHIYQGPESSLPGPPSISVQPPNVAAVRGYQPCVGYGGSRRQAGPRGGLALQPGQFSDPSQTCRVNGIKMEMPGQPHQLCSNVQSYSGQFYDQTVGFGQQDMKTGSFSMSEANCLLQGAGAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRAALPFPPLSLSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_005205661.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205661.3

ORF Insert Sequence:

ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG 
CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG 
CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC 
AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT 
TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT 
CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT 
CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC 
ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC 
CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG 
CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA 
GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT 
CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC 
TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG 
CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG 
ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA 
GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC 
TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA 
GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT 
CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC 
ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC 
ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC 
AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC 
CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG 
TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC 
AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC 
AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC 
CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG 
GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC 
TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC 
ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC 
AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC 
GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC 
GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC 
CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC 
GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA 
GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA 
GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG 
GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG 
GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG 
AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT 
GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC 
AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT 
ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG 
CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC 
CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG 
AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC 
TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG 
CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC 
GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC 
AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC 
GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT 
CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG 
AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA 
AGCAAATTCC TTGCGGTTAT GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_005205661.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205718.1 Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

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CloneID	OBa03954
Clone ID Related Accession (Same CDS sequence)	XM_001253425.4 , XM_005205661.3 , XM_005205661.4
Accession Version	XM_005205661.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205661.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG 
CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG 
CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC 
AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT 
TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT 
CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT 
CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC 
ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC 
CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG 
CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA 
GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT 
CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC 
TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG 
CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG 
ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA 
GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC 
TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA 
GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT 
CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC 
ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC 
ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC 
AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC 
CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG 
TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC 
AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC 
AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC 
CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG 
GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC 
TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC 
ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC 
AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC 
GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC 
GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC 
CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC 
GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA 
GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA 
GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG 
GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG 
GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG 
AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT 
GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC 
AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT 
ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG 
CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC 
CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG 
AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC 
TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG 
CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC 
GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC 
AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC 
GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT 
CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG 
AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA 
AGCAAATTCC TTGCGGTTAT GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205718.1
CDS	270..4856
Translation	MQPQIAPGLSKISEEPSTSSDERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDLVHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDVHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGGVGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMLPRLNPILPPKAPAVSPLIGNGTQPSTSCSLGGPMPLLPNRSDLSGVDITMLNMLNRRDSSTSTVSSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDGREPAGTLPPVHPPRRCSDGGAHGYSRRPLLPQDALGNGVRRASDPVRTVSESLSLPRVQRFNSLNSFNPPVLPPSLEKRNLVLQNYTRPDGGQARHFHSSPCPPSITENVTLESLTMDADVSLNDEDLLPDDVVQYLNSQNPAGYEQHFPGTFSDDSKVPHGLGSGPGDFDPLGLPDSHASHTGSQQYRSLEQPCPDASKSDLPIQWNEVSSGSADLSSSKLKCGQRSMGQQARAFGSYSHMGVHPQNPLRSGAGPAGGYPTLGEHASSFGGPEHLVSHGGGAGTNGNAFHEPPYKAQQYGNCLNRQPGAPTLLDGACGPGIQAAKLKSITMQGSGSHLDFGLSVVPNESTGSTVNGIPTPDPTGQGYLAQQLLGDSTHTQGAGRSGQNLLGQASATSHIYQGPESSLPGPPSISVQPPNVAAVRGYQPCVGYGGSRRQAGPRGGLALQPGQFSDPSQTCRVNGIKMEMPGQPHQLCSNVQSYSGQFYDQTVGFGQQDMKTGSFSMSEANCLLQGAGAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRAALPFPPLSLSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_005205661.4
Organism	Bos taurus(cattle)
Definition	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205661.4

ORF Insert Sequence:

ATGCAGCCAC AGATTGCCCC GGGGCTCAGC AAGATCAGCG AGGAGCCTTC AACATCCAGC 
GACGAGAGGG CCTCACTGAT CAAGAAGGAG ATCCATGGGT CCTTGCCCCA CCTGGCCGAG 
CCCTCTGTCC CCTACCGCGG GGCTGTGTTT GCTATGGACC CCAGGAATGG CTACATGGAG 
CCTCACTATC ACCCTCCTCA TCTCTTTCCT GCATTCCATC CTCCTGTACC AATTGATGCC 
AGACACCACG AGGGCCGTTA CCATTATGAT CCATCTCCGA TCCCTCCGTT GCATGTGCCT 
TCTGCCTTAT CTAGTAGCCC CACGTATTCT GACCTGCCCT TCATCAGGAT CTCCCCACAT 
CGGAACCCTG CTGCAGCTTC CGAATCCCCC TTCAGCCCCC CGCATCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTGCACAGC AGCCCATCCC TCTCCATGAT CTCGGCCGCT 
CGTGGGCTGA GCCCCACAGA TGCCCCCCAT GCCGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGTTAGCCGG CCAGCGCAGC CCCTATGCAG ACATCATTCC CTCTGCTGCC 
ACCGCTGGCG CAGGGGCCAT CCACATGGAG TATCTTCACG CCATGGATAG CACCAGATTC 
CCCAGCCCTA GGCTGTCAGC CCGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACAT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGCAG CAGCTCTTCA GCAAGTGGCT CCTATGGCCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCCT GAGCTTCACC TACCCTTCGG CGCCCGTGTC CCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGCTCAG CCTTTGGACA CAGCCCTCCG 
CTCATCCACC CCGCCCCAAC TTTCCCGACC CAGAGGCCTA TTCCCGGAAT CCCTACAGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCCGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGCAGTAC GGGGGACCTG GTGCACAACA AACGCTCCAA GATCAAACCA 
GATGAAGACC TCCCCAGCCC AGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTGAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCCGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGTTCTCGA GAGTTCGACA CCCAGGAGCA GCTGGTGCAT 
CATATAAATA ATGACCACAT CCATGGAGAG AAGAAGGAGT TTGTGTGCCG GTGGCTCGAC 
TGCTCCAGAG AACAGAAACC CTTCAAAGCC CAGTATATGC TGGTGGTGCA CATGCGCAGG 
CACACGGGGG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTCGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGAT GCAACAAGGC TTTCTCTAAT GCCTCGGATC GCGCTAAGCA CCAGAACAGG 
ACACATTCCA ACGAGAAACC ATATGTGTGC AAAATCCCCG GCTGCACCAA GCGCTACACA 
GACCCCAGCT CCCTCCGGAA ACACGTGAAG ACCGTGCACG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACGT GCATCCCCGG CCCCCGCCCC CTCGAGATTC CGGCAGCCAC 
TCACAGTCTA GGTCACCTGG CCGGCAGACT CAGGGAGCCC TCGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAACCGTCAA GGCGGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TGGTGTGGGC 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC TGCAACCACA 
GCCCTCGCTT TGCAAGCCCG GAGGAACCCA GCAGGGACCA AATGGATGGA GCACGTCAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACGC AGCCCAGTAC CAGCTGCAGT 
CTGGGCGGGC CCATGCCCCT CCTCCCGAAC AGAAGCGACC TGTCTGGGGT GGACATCACC 
ATGTTGAACA TGCTCAACCG GAGGGACAGC AGCACCAGCA CCGTCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCTGG CATCTCGCCC TGCTTCTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGGCGACC CCAGAACGTG AGCGTGGCCG ACTCTTACGA CCCCATCTCC 
ACCGACGCCT CTCGCAGGTC CAGCGAGGCC AGCCAGGGCG ACGGTCTGCC CAGCCTGCTC 
AGCCTCACTC CCGCCCAGCA GTACCGCCTG AAAGCCAAGT ACGCTGCCGC CACGGGCGGC 
CCCCCGCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCCG GATGGCCCTG 
TTGGGAGATG GCAGGGAGCC GGCGGGGACC CTGCCTCCCG TGCATCCTCC TCGGAGATGC 
AGCGACGGCG GGGCCCACGG TTACAGCCGG CGCCCACTGC TGCCCCAGGA TGCGCTGGGC 
AACGGCGTGA GAAGAGCCAG CGACCCGGTG AGGACGGTTT CGGAGAGCCT CTCTCTGCCC 
CGGGTACAGC GGTTCAACAG CCTTAACAGC TTCAACCCTC CGGTGTTGCC TCCATCCCTG 
GAAAAGCGCA ACCTCGTGCT TCAGAACTAC ACGCGGCCCG ATGGGGGCCA GGCCCGCCAC 
TTCCACTCCT CTCCCTGTCC CCCGAGCATC ACTGAGAATG TCACCCTGGA GTCCCTGACC 
ATGGACGCCG ACGTGAGCCT GAACGATGAG GACCTCCTGC CTGATGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCCAGC CGGGTATGAG CAGCACTTCC CAGGCACCTT CTCTGATGAC 
AGCAAAGTGC CCCATGGCTT AGGGAGTGGA CCCGGGGACT TTGACCCACT GGGGCTGCCC 
GACAGCCACG CCAGCCACAC CGGCAGCCAG CAGTACCGCA GCCTGGAGCA GCCCTGCCCC 
GATGCCAGTA AATCCGACCT GCCCATTCAG TGGAACGAAG TCAGCTCCGG CAGCGCGGAC 
CTGTCCTCCT CCAAGCTGAA ATGTGGCCAG CGGTCCATGG GGCAGCAGGC CCGGGCCTTC 
GGGTCGTACA GCCACATGGG TGTTCACCCA CAGAATCCAT TGAGGAGTGG GGCTGGCCCA 
GCTGGGGGGT ATCCGACCCT TGGGGAGCAT GCCAGCTCCT TCGGGGGCCC GGAACACTTA 
GTGAGCCACG GTGGCGGAGC TGGCACCAAT GGGAATGCCT TCCATGAACC ACCCTATAAG 
GCCCAGCAGT ATGGGAACTG CCTCAACAGG CAGCCTGGTG CCCCCACCTT ACTCGATGGG 
GCCTGTGGCC CTGGGATCCA GGCGGCAAAG CTGAAAAGCA TCACCATGCA AGGGAGCGGG 
AGCCATCTGG ATTTTGGCCT GTCGGTGGTG CCGAATGAGT CAACCGGCAG CACGGTGAAT 
GGCATACCCA CCCCAGACCC CACAGGACAG GGGTACCTGG CTCAGCAGCT CCTCGGTGAC 
AGCACACACA CCCAGGGGGC AGGCCGCTCC GGGCAGAACC TGCTGGGGCA GGCTAGTGCT 
ACCTCACACA TCTATCAAGG GCCGGAGAGC AGCCTGCCCG GGCCTCCCAG CATCAGCGTG 
CAGCCGCCAA ACGTGGCAGC CGTCAGGGGC TACCAGCCAT GTGTTGGCTA CGGGGGCAGC 
CGGCGCCAGG CTGGGCCGAG GGGTGGTCTC GCTCTGCAGC CAGGACAGTT TAGTGACCCG 
AGTCAGACCT GCAGGGTGAA TGGCATCAAG ATGGAGATGC CAGGACAGCC CCATCAGCTC 
TGTTCGAATG TGCAGAGTTA CTCTGGTCAG TTCTATGACC AAACTGTTGG CTTCGGTCAG 
CAAGACATGA AAACTGGTTC CTTCTCCATG TCAGAAGCCA ACTGCCTGCT CCAGGGGGCC 
GGCGCCGAAA ACTCTGAATT ACTCTCCCCA GGTGCTAACC AGGTGACAAG CACAGTTGAC 
AGCCTCGACA GCCATGACCT GGAAGGGGTG CAGATTGATT TCGACGCTAT CATAGACGAC 
GGGGACCACG CCAGCCTGAT GTCAGGAGCC CTGAGCCCAA GCATTATTCA GAACCTTTCT 
CATAGCTCCT CCCGACTCAC CACGCCGCGA GCCGCCCTTC CGTTCCCGCC TCTGTCTTTG 
AGCACCACCA ACATGGCAAT CGGGGACATG AGCTCTCTGC TGACCTCCCT CGCGGAAGAA 
AGCAAATTCC TTGCGGTTAT GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_015463085.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318571.1 Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OBa18737
Clone ID Related Accession (Same CDS sequence)	XM_015463085.1 , XM_005205659.3 , XM_005205659.4
Accession Version	XM_015463085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC 
GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC 
TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT 
CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG 
AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT 
GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC 
GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC 
AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT 
GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG 
GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT 
ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC 
TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC 
ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA 
CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC 
CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA 
CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC 
CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA 
GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG 
GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG 
TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC 
AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC 
CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC 
GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC 
CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC 
CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG 
GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC 
GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC 
GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG 
GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA 
AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC 
CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG 
GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC 
AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC 
AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT 
GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG 
TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG 
TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT 
GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG 
AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC 
CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC 
TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC 
CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC 
ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC 
ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC 
TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG 
CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG 
CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT 
CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT 
TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA 
GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC 
GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC 
CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG 
GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT 
AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC 
ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC 
AAATTCCTTG CGGTTATGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318571.1
CDS	229..4992
Translation	MEAQSQSSTTTEKKKVENSIVKCPTRPDVSEKAVASSTTSNEDESPGQTYHRERRNAVTMQPQIAPGLSKISEEPSTSSDERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDLVHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDVHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGGVGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMLPRLNPILPPKAPAVSPLIGNGTQPSTSCSLGGPMPLLPNRSDLSGVDITMLNMLNRRDSSTSTVSSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDGREPAGTLPPVHPPRRCSDGGAHGYSRRPLLPQDALGNGVRRASDPVRTVSESLSLPRVQRFNSLNSFNPPVLPPSLEKRNLVLQNYTRPDGGQARHFHSSPCPPSITENVTLESLTMDADVSLNDEDLLPDDVVQYLNSQNPAGYEQHFPGTFSDDSKVPHGLGSGPGDFDPLGLPDSHASHTGSQQYRSLEQPCPDASKSDLPIQWNEVSSGSADLSSSKLKCGQRSMGQQARAFGSYSHMGVHPQNPLRSGAGPAGGYPTLGEHASSFGGPEHLVSHGGGAGTNGNAFHEPPYKAQQYGNCLNRQPGAPTLLDGACGPGIQAAKLKSITMQGSGSHLDFGLSVVPNESTGSTVNGIPTPDPTGQGYLAQQLLGDSTHTQGAGRSGQNLLGQASATSHIYQGPESSLPGPPSISVQPPNVAAVRGYQPCVGYGGSRRQAGPRGGLALQPGQFSDPSQTCRVNGIKMEMPGQPHQLCSNVQSYSGQFYDQTVGFGQQDMKTGSFSMSEANCLLQGAGAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRAALPFPPLSLSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015463085.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463085.1

ORF Insert Sequence:

ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC 
GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC 
TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT 
CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG 
AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT 
GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC 
GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC 
AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT 
GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG 
GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT 
ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC 
TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC 
ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA 
CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC 
CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA 
CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC 
CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA 
GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG 
GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG 
TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC 
AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC 
CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC 
GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC 
CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC 
CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG 
GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC 
GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC 
GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG 
GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA 
AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC 
CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG 
GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC 
AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC 
AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT 
GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG 
TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG 
TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT 
GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG 
AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC 
CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC 
TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC 
CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC 
ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC 
ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC 
TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG 
CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG 
CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT 
CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT 
TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA 
GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC 
GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC 
CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG 
GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT 
AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC 
ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC 
AAATTCCTTG CGGTTATGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_005205659.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205716.1 Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OBa18737
Clone ID Related Accession (Same CDS sequence)	XM_015463085.1 , XM_005205659.3 , XM_005205659.4
Accession Version	XM_005205659.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC 
GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC 
TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT 
CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG 
AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT 
GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC 
GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC 
AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT 
GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG 
GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT 
ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC 
TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC 
ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA 
CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC 
CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA 
CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC 
CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA 
GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG 
GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG 
TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC 
AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC 
CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC 
GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC 
CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC 
CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG 
GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC 
GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC 
GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG 
GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA 
AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC 
CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG 
GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC 
AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC 
AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT 
GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG 
TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG 
TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT 
GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG 
AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC 
CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC 
TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC 
CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC 
ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC 
ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC 
TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG 
CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG 
CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT 
CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT 
TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA 
GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC 
GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC 
CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG 
GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT 
AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC 
ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC 
AAATTCCTTG CGGTTATGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205716.1
CDS	229..4992
Translation	MEAQSQSSTTTEKKKVENSIVKCPTRPDVSEKAVASSTTSNEDESPGQTYHRERRNAVTMQPQIAPGLSKISEEPSTSSDERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDLVHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDVHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGGVGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMLPRLNPILPPKAPAVSPLIGNGTQPSTSCSLGGPMPLLPNRSDLSGVDITMLNMLNRRDSSTSTVSSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDGREPAGTLPPVHPPRRCSDGGAHGYSRRPLLPQDALGNGVRRASDPVRTVSESLSLPRVQRFNSLNSFNPPVLPPSLEKRNLVLQNYTRPDGGQARHFHSSPCPPSITENVTLESLTMDADVSLNDEDLLPDDVVQYLNSQNPAGYEQHFPGTFSDDSKVPHGLGSGPGDFDPLGLPDSHASHTGSQQYRSLEQPCPDASKSDLPIQWNEVSSGSADLSSSKLKCGQRSMGQQARAFGSYSHMGVHPQNPLRSGAGPAGGYPTLGEHASSFGGPEHLVSHGGGAGTNGNAFHEPPYKAQQYGNCLNRQPGAPTLLDGACGPGIQAAKLKSITMQGSGSHLDFGLSVVPNESTGSTVNGIPTPDPTGQGYLAQQLLGDSTHTQGAGRSGQNLLGQASATSHIYQGPESSLPGPPSISVQPPNVAAVRGYQPCVGYGGSRRQAGPRGGLALQPGQFSDPSQTCRVNGIKMEMPGQPHQLCSNVQSYSGQFYDQTVGFGQQDMKTGSFSMSEANCLLQGAGAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRAALPFPPLSLSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_005205659.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205659.3

ORF Insert Sequence:

ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC 
GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC 
TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT 
CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG 
AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT 
GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC 
GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC 
AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT 
GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG 
GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT 
ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC 
TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC 
ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA 
CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC 
CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA 
CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC 
CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA 
GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG 
GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG 
TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC 
AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC 
CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC 
GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC 
CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC 
CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG 
GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC 
GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC 
GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG 
GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA 
AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC 
CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG 
GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC 
AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC 
AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT 
GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG 
TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG 
TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT 
GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG 
AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC 
CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC 
TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC 
CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC 
ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC 
ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC 
TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG 
CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG 
CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT 
CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT 
TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA 
GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC 
GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC 
CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG 
GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT 
AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC 
ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC 
AAATTCCTTG CGGTTATGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_005205659.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205716.1 Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OBa18737
Clone ID Related Accession (Same CDS sequence)	XM_015463085.1 , XM_005205659.3 , XM_005205659.4
Accession Version	XM_005205659.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC 
GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC 
TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT 
CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG 
AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT 
GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC 
GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC 
AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT 
GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG 
GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT 
ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC 
TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC 
ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA 
CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC 
CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA 
CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC 
CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA 
GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG 
GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG 
TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC 
AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC 
CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC 
GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC 
CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC 
CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG 
GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC 
GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC 
GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG 
GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA 
AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC 
CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG 
GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC 
AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC 
AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT 
GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG 
TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG 
TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT 
GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG 
AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC 
CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC 
TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC 
CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC 
ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC 
ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC 
TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG 
CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG 
CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT 
CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT 
TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA 
GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC 
GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC 
CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG 
GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT 
AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC 
ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC 
AAATTCCTTG CGGTTATGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205716.1
CDS	123..4886
Translation	MEAQSQSSTTTEKKKVENSIVKCPTRPDVSEKAVASSTTSNEDESPGQTYHRERRNAVTMQPQIAPGLSKISEEPSTSSDERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDLVHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDVHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGGVGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMLPRLNPILPPKAPAVSPLIGNGTQPSTSCSLGGPMPLLPNRSDLSGVDITMLNMLNRRDSSTSTVSSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDGREPAGTLPPVHPPRRCSDGGAHGYSRRPLLPQDALGNGVRRASDPVRTVSESLSLPRVQRFNSLNSFNPPVLPPSLEKRNLVLQNYTRPDGGQARHFHSSPCPPSITENVTLESLTMDADVSLNDEDLLPDDVVQYLNSQNPAGYEQHFPGTFSDDSKVPHGLGSGPGDFDPLGLPDSHASHTGSQQYRSLEQPCPDASKSDLPIQWNEVSSGSADLSSSKLKCGQRSMGQQARAFGSYSHMGVHPQNPLRSGAGPAGGYPTLGEHASSFGGPEHLVSHGGGAGTNGNAFHEPPYKAQQYGNCLNRQPGAPTLLDGACGPGIQAAKLKSITMQGSGSHLDFGLSVVPNESTGSTVNGIPTPDPTGQGYLAQQLLGDSTHTQGAGRSGQNLLGQASATSHIYQGPESSLPGPPSISVQPPNVAAVRGYQPCVGYGGSRRQAGPRGGLALQPGQFSDPSQTCRVNGIKMEMPGQPHQLCSNVQSYSGQFYDQTVGFGQQDMKTGSFSMSEANCLLQGAGAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRAALPFPPLSLSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_005205659.4
Organism	Bos taurus(cattle)
Definition	Bos taurus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205659.4

ORF Insert Sequence:

ATGGAGGCCC AGTCCCAGAG CTCGACGACC ACTGAGAAAA AGAAAGTGGA GAATTCCATA 
GTGAAATGCC CCACTCGGCC AGATGTCAGC GAGAAAGCCG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA TTGCCCCGGG GCTCAGCAAG ATCAGCGAGG AGCCTTCAAC ATCCAGCGAC 
GAGAGGGCCT CACTGATCAA GAAGGAGATC CATGGGTCCT TGCCCCACCT GGCCGAGCCC 
TCTGTCCCCT ACCGCGGGGC TGTGTTTGCT ATGGACCCCA GGAATGGCTA CATGGAGCCT 
CACTATCACC CTCCTCATCT CTTTCCTGCA TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CACCACGAGG GCCGTTACCA TTATGATCCA TCTCCGATCC CTCCGTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCCAC GTATTCTGAC CTGCCCTTCA TCAGGATCTC CCCACATCGG 
AACCCTGCTG CAGCTTCCGA ATCCCCCTTC AGCCCCCCGC ATCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT GCACAGCAGC CCATCCCTCT CCATGATCTC GGCCGCTCGT 
GGGCTGAGCC CCACAGATGC CCCCCATGCC GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGT TAGCCGGCCA GCGCAGCCCC TATGCAGACA TCATTCCCTC TGCTGCCACC 
GCTGGCGCAG GGGCCATCCA CATGGAGTAT CTTCACGCCA TGGATAGCAC CAGATTCCCC 
AGCCCTAGGC TGTCAGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCA AGTGGCTCCT ATGGCCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCGGCGC CCGTGTCCCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGCTCAGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCCG CCCCAACTTT CCCGACCCAG AGGCCTATTC CCGGAATCCC TACAGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCC GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGTACGGG GGACCTGGTG CACAACAAAC GCTCCAAGAT CAAACCAGAT 
GAAGACCTCC CCAGCCCAGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG 
GTGAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CCGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG TTCTCGAGAG TTCGACACCC AGGAGCAGCT GGTGCATCAT 
ATAAATAATG ACCACATCCA TGGAGAGAAG AAGGAGTTTG TGTGCCGGTG GCTCGACTGC 
TCCAGAGAAC AGAAACCCTT CAAAGCCCAG TATATGCTGG TGGTGCACAT GCGCAGGCAC 
ACGGGGGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTC 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGATGCA ACAAGGCTTT CTCTAATGCC TCGGATCGCG CTAAGCACCA GAACAGGACA 
CATTCCAACG AGAAACCATA TGTGTGCAAA ATCCCCGGCT GCACCAAGCG CTACACAGAC 
CCCAGCTCCC TCCGGAAACA CGTGAAGACC GTGCACGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACGTGCA TCCCCGGCCC CCGCCCCCTC GAGATTCCGG CAGCCACTCA 
CAGTCTAGGT CACCTGGCCG GCAGACTCAG GGAGCCCTCG GGGAGCAGAA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAA CCGTCAAGGC GGAGAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTGG TGTGGGCGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACTGC AACCACAGCC 
CTCGCTTTGC AAGCCCGGAG GAACCCAGCA GGGACCAAAT GGATGGAGCA CGTCAAACTA 
GAAAGGCTCA AACAAGTGAA TGGAATGCTT CCACGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCAG TCTCTCCTCT CATAGGAAAC GGCACGCAGC CCAGTACCAG CTGCAGTCTG 
GGCGGGCCCA TGCCCCTCCT CCCGAACAGA AGCGACCTGT CTGGGGTGGA CATCACCATG 
TTGAACATGC TCAACCGGAG GGACAGCAGC ACCAGCACCG TCAGCTCCGC CTACCTGAGC 
AGCCGCCGCT CCTCTGGCAT CTCGCCCTGC TTCTCCAGCC GCCGGTCCAG CGAGGCGTCC 
CAGGCCGAGG GGCGACCCCA GAACGTGAGC GTGGCCGACT CTTACGACCC CATCTCCACC 
GACGCCTCTC GCAGGTCCAG CGAGGCCAGC CAGGGCGACG GTCTGCCCAG CCTGCTCAGC 
CTCACTCCCG CCCAGCAGTA CCGCCTGAAA GCCAAGTACG CTGCCGCCAC GGGCGGCCCC 
CCGCCCACCC CGCTGCCCAA CATGGAGAGG ATGAGCCTCA AGACCCGGAT GGCCCTGTTG 
GGAGATGGCA GGGAGCCGGC GGGGACCCTG CCTCCCGTGC ATCCTCCTCG GAGATGCAGC 
GACGGCGGGG CCCACGGTTA CAGCCGGCGC CCACTGCTGC CCCAGGATGC GCTGGGCAAC 
GGCGTGAGAA GAGCCAGCGA CCCGGTGAGG ACGGTTTCGG AGAGCCTCTC TCTGCCCCGG 
GTACAGCGGT TCAACAGCCT TAACAGCTTC AACCCTCCGG TGTTGCCTCC ATCCCTGGAA 
AAGCGCAACC TCGTGCTTCA GAACTACACG CGGCCCGATG GGGGCCAGGC CCGCCACTTC 
CACTCCTCTC CCTGTCCCCC GAGCATCACT GAGAATGTCA CCCTGGAGTC CCTGACCATG 
GACGCCGACG TGAGCCTGAA CGATGAGGAC CTCCTGCCTG ATGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCCAGCCGG GTATGAGCAG CACTTCCCAG GCACCTTCTC TGATGACAGC 
AAAGTGCCCC ATGGCTTAGG GAGTGGACCC GGGGACTTTG ACCCACTGGG GCTGCCCGAC 
AGCCACGCCA GCCACACCGG CAGCCAGCAG TACCGCAGCC TGGAGCAGCC CTGCCCCGAT 
GCCAGTAAAT CCGACCTGCC CATTCAGTGG AACGAAGTCA GCTCCGGCAG CGCGGACCTG 
TCCTCCTCCA AGCTGAAATG TGGCCAGCGG TCCATGGGGC AGCAGGCCCG GGCCTTCGGG 
TCGTACAGCC ACATGGGTGT TCACCCACAG AATCCATTGA GGAGTGGGGC TGGCCCAGCT 
GGGGGGTATC CGACCCTTGG GGAGCATGCC AGCTCCTTCG GGGGCCCGGA ACACTTAGTG 
AGCCACGGTG GCGGAGCTGG CACCAATGGG AATGCCTTCC ATGAACCACC CTATAAGGCC 
CAGCAGTATG GGAACTGCCT CAACAGGCAG CCTGGTGCCC CCACCTTACT CGATGGGGCC 
TGTGGCCCTG GGATCCAGGC GGCAAAGCTG AAAAGCATCA CCATGCAAGG GAGCGGGAGC 
CATCTGGATT TTGGCCTGTC GGTGGTGCCG AATGAGTCAA CCGGCAGCAC GGTGAATGGC 
ATACCCACCC CAGACCCCAC AGGACAGGGG TACCTGGCTC AGCAGCTCCT CGGTGACAGC 
ACACACACCC AGGGGGCAGG CCGCTCCGGG CAGAACCTGC TGGGGCAGGC TAGTGCTACC 
TCACACATCT ATCAAGGGCC GGAGAGCAGC CTGCCCGGGC CTCCCAGCAT CAGCGTGCAG 
CCGCCAAACG TGGCAGCCGT CAGGGGCTAC CAGCCATGTG TTGGCTACGG GGGCAGCCGG 
CGCCAGGCTG GGCCGAGGGG TGGTCTCGCT CTGCAGCCAG GACAGTTTAG TGACCCGAGT 
CAGACCTGCA GGGTGAATGG CATCAAGATG GAGATGCCAG GACAGCCCCA TCAGCTCTGT 
TCGAATGTGC AGAGTTACTC TGGTCAGTTC TATGACCAAA CTGTTGGCTT CGGTCAGCAA 
GACATGAAAA CTGGTTCCTT CTCCATGTCA GAAGCCAACT GCCTGCTCCA GGGGGCCGGC 
GCCGAAAACT CTGAATTACT CTCCCCAGGT GCTAACCAGG TGACAAGCAC AGTTGACAGC 
CTCGACAGCC ATGACCTGGA AGGGGTGCAG ATTGATTTCG ACGCTATCAT AGACGACGGG 
GACCACGCCA GCCTGATGTC AGGAGCCCTG AGCCCAAGCA TTATTCAGAA CCTTTCTCAT 
AGCTCCTCCC GACTCACCAC GCCGCGAGCC GCCCTTCCGT TCCCGCCTCT GTCTTTGAGC 
ACCACCAACA TGGCAATCGG GGACATGAGC TCTCTGCTGA CCTCCCTCGC GGAAGAAAGC 
AAATTCCTTG CGGTTATGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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GLI3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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