Oligo pools are collections of oligonucleotides synthesized using GenScript’s proprietary high density Complementary Metal Oxide Semiconductor (CMOS) technology. GenScript oligo pools are designed for PCR (Polymerase Chain Reaction) amplification, which requires that the oligos be carefully designed with specific sequences at their ends to ensure effective primer annealing and successful amplification.
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![Oligo Pool]( "Oligo Pool")
Overview
At GenScript, our Oligo Pool service offers researchers a comprehensive solution for customized oligonucleotide pools. Using our proprietary high density Complementary Metal Oxide Semiconductor (CMOS) technology, we achieve unmatched precision via microelectrode control, ensuring even distribution and minimal batch differences. Harnessing the industry's largest capacity, with over 8 million oligos on a single chip, our technology empowers breakthroughs in gene editing, protein/antibody engineering, DNA data storage, and more.
Consistency
Complete chamber reaction for excellent uniformity
Precision
Precise control of current and voltage at each electrode
Capacity
Up to 8 Million Oligos on a single chip
Technology
Behind the TitansDiscover how our Next-Generation CMOS Technology is pushing the boundaries of DNA Synthesis.
Next-Generation MSC DNA Synthesis Technology
Miniature Semiconductor Chip (MSC) enables large-scale de novo synthesis of DNA using proprietary electrochemistry technologies and delivers precision on a microelectrode scale.
1/ACCURATE SPATIAL CONTROL
Semiconductor based ensures spatial control over the whole process
2/ENCLOSED ENVIRONMENT
Less interference of H2O, O2, O3 and static electricity
3/CONSISTENCY & UNIFORMITY
Full chamber reaction with standard wafer and chip production process
Services Specification
GenScript Oligo Pool Service Specification Chip Size 8 Million Oligos on 1 Chip Oligo Length Up to 170nt Yield Up to 1 fmol for each oligo Delivery Time 1-2 weeks
Quality Control - Visual verification of synthesized DNA for each electrode using a microarray scanner
- Full-spectrum analysis to assess purity and yield
- Guaranteed final yield of synthesized oligos in each pool
- NEW Verification of full oligo length synthesis through the use of spike-in control oligos
*PCR amplification of entire pool, cloning, and sequencing for sequence coverage and accuracy at an additional fee*
We Offer Customized Pricing to Match your Needs (Starting from 1K pool size). Get an Estimated Price with Our Calculator Today!
Applications
Genome Editing 1NGS Target Enrichment 2Protein & Antibody Engineering 3Gene Fragments 4DNA Data Storage 5Genome Editing
CRISPR gRNA Library
Oligo pool gRNA libraries, utilizing CRISPR gene editing, enable high-throughput screening for cancer therapy, diagnostics, and microorganism engineering. They facilitate gene knock-out and activation, probing tumorigenesis, biomarkers, and metabolic pathways. This transformative tool leverages CRISPR's precision for versatile biomedical and biotech applications.
NGS Target Enrichment
NGS Target Enrichment
Next-generation sequencing (NGS) target enrichment utilizing oligo pools has emerged as a transformative technique in genomics research. By custom designing pools of oligonucleotide probes complementary to specific genomic regions of interest, researchers can efficiently capture and amplify relevant DNA fragments for sequencing. This approach not only streamlines the sequencing process but also significantly reduces the cost and data burden.
Protein & Antibody Engineering
Mutant Libraries
Mutant libraries are powerful, high-throughput tools for optimizing protein structure and function. However, traditional methods for library construction, including error-prone PCR or degenerate methods, suffer from limited control over codon usage and poor variant representation. Powered by our semiconductor-based oligo synthesis technology, our Precision Mutant Library service offers precise control over each synthesized variant. The result is a more diverse and fully covered mutant library with unbiased distribution.
Gene Fragments
Gene Fragments
Gene Fragments offer a cost-effective, swift, and effective avenue for constructing the requisite genes in your research endeavors. These double-stranded DNA fragments of up to 3,000 base pairs in length, fulfilling the demands of easy gene construction or modification suitable for a variety of applications. The Gene Fragments Service leverages our exclusive complementary metal oxide semiconductor (CMOS) integrated circuits (IC), ensuring unmatched precision, accelerated delivery schedules, and exceptional cost-effectiveness.
DNA Data Storage
DNA Data Storage
DNA data storage involves encoding digital information as DNA sequences, utilizing the four DNA bases (A, T, C, and G) to represent binary data (0s and 1s). These sequences can be later synthesized into DNA strands for storage. Oligo pools are used to generate these sequences efficiently and in parallel, allowing large amounts of data to be encoded using unique combinations of oligos.
Resource
sgRNA Library Application Note
Building a diverse CRISPR gRNA library using arrayed-synthesized oligo pools.
NGS Target Enrichment Application
Double stranded DNA capture probes in NGS targeted sequencing.
Protein & Antibody Engineering Application
Assembly of precise mutant libraries using arrayed-synthesized oligos.
Oligo Pools Brochure
Learn how GenSript Oligo Pool enables precise, scalable library construction and high-throughput screening.
FAQ
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Q1. What are GenScript Oligo Pools, and how are they intended to be used?
- Q2. What does GenScript guarantee when it comes to the oligo pools?
GenScript guarantees that when an oligo pool is appropriately designed and used as a template for PCR with suitable primers, it will produce a PCR product of the correct size. However, it's important to note that while general PCR guidelines are provided, modifications to the PCR protocols might be necessary to accommodate specific variables, such as the choice of primers and the presence of certain sequence motifs. As every pool is uniquely made according to the customer’s design, GenScript cannot commit to specific characteristics that could be influenced by the sequence design or downstream workflow.
- Q3. Is there a user guide for PCR enrichment and amplification of Oligo Pools?
Yes. You can download the GenScript’s Oligo Pool User Guide and PCR Amplification Guidelines to learn about PCR enrichment and amplification best practices for Oligo Pools.
- Q4: How does GenScript Oligo Pool verify the full-length synthesis of oligos?
GenScript Oligo Pool offers a free QC option using PCR amplification of spike-in control oligos synthesized on the same chip. Each pool includes 50 optimized control oligos that are amplified to confirm full-length synthesis accuracy. Customer sequences are screened to ensure QC primers only target these control oligos.
New – Synthesis verification of full oligo length using spike-in control oligos
In order to verify full-length synthesis of oligos, we now offer an additional QC option for FREE, PCR amplification of control oligos synthesized on the same chip . Sequences of 50 optimized control oligos will be synthesized with each pool and PCR amplification of the control oligos will be performed to confirm oligo synthesis. Customer sequences will be screened to ensure QC PCR primers will only capture control oligos.
Synthesis Verification of Full Length Oligos in a Pool
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- Q2. What does GenScript guarantee when it comes to the oligo pools?
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