Trimer Oligonucleotides Synthesis Services

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Overview

Trimer oligonucleotides are trimer phosphoramidites formed by connecting three predetermined nucleotides in a specific order corresponding to different amino acids. By using various trimer oligonucleotides as synthetic raw materials and adding them at predetermined positions, a DNA library that conforms to a predetermined sequence can be obtained.

GenScript provides DNA library synthesis services using trimer oligonucleotides as raw materials. These can be used for downstream library construction applications, supporting subsequent research in protein-directed evolution, antibody or peptide drug screening, enzyme optimization, AAV engineering, drug target screening and discovery, and more.

Advantages

Precise and Customized Synthesis

Avoid unexpected mutations and stop codons.
Support up to 20 mutation locus & custom AA ratio mutation

100% Codon Coverage and Lower Deviation

Ensure library diversity and homogeneity
Support codon content ratio as low as 1%

Library capacity up to 10¹⁰ & Multiple Forms

Can be directly cloned into customer vectors
Oligos, PCR product, plasmid, glycerol stock is optional

Cost-effective and Fast Delivery

Self-produced trimer phosphoramidites materials
Save cost for high diversity libraries & as fast as 2 weeks

Oligo Synthesis

Service

Trimer Oligo Length	Number of amino acid sites *	Purification	Quantity	Delivery Time	Deliverables #
≤110nt	≤15	HPLC	≥90 nt: Approx. 1 nmol <90 nt: Approx. 2 nmol	2-3 weeks	Lyophilized powder (mixed oligo)

* Support E.coli and Saccharomyces cerevisiae expression systems
# Deliverables such as PCR products, plasmids, and glycerol bacterial stocks are optional

Default - Molecular Weight, Quantification
Optional - NGS Sequencing

Case Study

Proportional mutation with high uniform distribution
Case 1. The target protein has 349 AA and needs saturation mutations at 5 sites, theoretical diversity is 20⁵ = 3.2 X 10⁶

Results:
- The sequence coverage is 100%
- The proportional distribution of 20 amino acids at 5 saturation mutation sites is highly uniform
Case 2. The target protein has 123 AA and needs saturation mutations at 2 sites, theoretical diversity is 20² = 400

Results:
- The sequence coverage is 100%
- The distribution of mutations is highly uniform as 90% / 10% as low as 1.91
Non-proportional mutation matches customized request
Case 3. The target protein needs a different ratio of mutations at 11 sites

Results:
- Amino acid distribution in diverse ratios highly match with design proportions, with low deviation

Applications

A DNA library synthesized using trimer oligonucleotides can be utilized to construct mutant libraries for further screening and research as shown below. This may be a more cost-effective and flexible solution.

Antibody Discovery

Peptide Drug Discovery

Enzyme Optimization

CAR/TCR Engineering

AAV Engineering

Overview

DNA mutant libraries are used in antibody engineering to generate diverse antibody variants with targeted mutations. These libraries enable high-throughput screening to identify variants with enhanced binding affinity and stability, accelerating the optimization and development of therapeutic antibodies.

Overview

DNA mutant libraries enable high-throughput screening to find enzyme variants with enhanced catalytic efficiency, stability, and much more, speeding up the development of improved biocatalysts for industrial and pharmaceutical applications.

Overview

DNA mutant libraries enable high-throughput screening to find CAR/TCR variants with enhanced antigen recognition, signaling, and costimulatory features, accelerating the development of improved immunotherapies for cancer and other diseases.

Overview

DNA mutant libraries enable high-throughput screening to find AAV variants with optimized capsid, genomic payload, and regulatory elements, accelerating the development of advanced gene therapies for a wide range of genetic disorders.

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GenScript Trimer Oligonucleotides Synthesis

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EMAIL

oligo@genscript.com

PHONE

1-877-436-7274

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Trimer Oligonucleotides Synthesis

On-stop Solution with Self-produced Trimer Phosphoramidites 100% Codon Coverage and Lower Deviation

Overview

Advantages

Service

Case Study

Applications

Get in Touch with GenScript Trimer Oligonucleotides Synthesis

REQUEST A QUOTE

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ONLINE FORM

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Get in Touch with
GenScript Trimer Oligonucleotides Synthesis