Oligonucleotide Purification

Purification with reverse-phase silica gel column which can effectively remove salts. The purity can meet the needs of most molecular biology experiments.

Advantages

• Fast
• High throughput
• Cost-effective

Applications

PCR amplification, gene synthesis, DNA sequencing, NGS capture probes, NGS Multiplex PCR oligos

The purification of oligo with HPLC can achieve high purity and is mainly used for short chain (< 40 nt) and modified oligo.

Advantages

• Purity can reach > 90%
• Remove N-1 short fragment effectively
• Purification for modified oligo with hydrophobic groups

Applications

Point mutation, subcloning, diagnostic PCR, protein binding gel migration electrophoresis analysis, modified primers with hydrophobic groups.

Using denaturing polyacrylamide gel electrophoresis, oligo of different molecular weights migrate at different speeds during electrophoresis, allowing for separation. Finally, the target DNA is recovered from the gel.

Advantages

• Purity can be > 90%
• Effective for purification of long-stranded oligo( 151 – 200 nt )

Applications

Mutation libraries, protein binding gel migration electrophoresis analysis, modified oligos such as phosphate and biotin

Utilizing oligonucleotide purification columns and other methods to remove short sequences and impurities from the product. By using automated equipment, DNA purification and recovery can be completed efficiently in batches.

Advantages

• Fast, cost-effective
• Similar purity as PAGE within 40 nt

Applications

• Multiplex PCR, point mutation, gene synthesis

HPLC purification done in a clean room. Before purification of each oligo, columns are thoroughly cleared of residues by multiple special rinsing procedures, and different oligos are kept reasonably spaced apart and purified separately to avoid exogenous contamination.

Advantages

• Strict control of exogenous contamination
• No NTC peaks within 40 cycles guaranteed

Applications

• TaqMan probes, qPCR oligos and commercial diagnostic oligo
• Suitable for large-scale production for ＞100 nmol per sequence

PAGE purification done in a clean room.The target sequences are separated by denaturing polyacrylamide gel electrophoresis, and each oligo is purified with an independent purification system and disposable consumables to avoid the possibility of mixing with other oligos.

Advantages

• Cross-contamination rate as low as 0.01%

Applications

• Commercial diagnostic oligos ( 151 – 200 nt )