Recombinant Antibody Production with TurboCHO™ Express Platform

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Overview

GenScript has developed the proprietary TurboCHO™ expression system for higher-titer production of recombinant antibodies. Unlike TurboCHO™ High throughput platform, TurboCHO™ Express is designed for middle-scale production, with a timeline from gene synthesis to antibody delivery of just 10 business days! It also offers multiple purification and endotoxin control options in the default QC list, ensuring high-quality product delivery. TurboCHO™ Express platform can also deliver a range of purified antibody products (e.g., IgGs, Fab, scFV, VHH, bi-specific antibodies, and more) in quantities (i.e., from 5 mg to 1 g).

Additionally, GenScript introduces the FucoFree Cell line, designed for the production of afucosylated antibodies. This innovative cell line eliminates fucose modification, resulting in enhanced antibody-dependent cellular cytotoxicity (ADCC) and improved therapeutic efficacy. The combination of TurboCHO™ Express and the FucoFree Cell line provides researchers with efficient and tailored antibody production, offering new possibilities in the field of antibody therapeutics.

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Protein Services

Service Highlights

Cost-Effective

Superior Quality

Easy to Scale-up

FucoFree Cell Available

General Workflow

From gene synthesis to delivery workflow

Service Specifications

Perspective	Description
Targeting Amount (mAb)	Up to 1 g Other amounts upon request
Turnaround Time	Starting from 10 business days
Target Types	Recombinant proteins Antibody formats including full length antibody, bi-specific antibody, Fabs, scFvs, VHHs, or other fragments fused with Fc
QC	A280 SDS-PAGE/CE-SDS (extra charge) SEC-HPLC Endotoxin control
Deliverables	Target Purity: Antibody≥95% Target Endotoxin Level: <0.5EU /mg
Additional Services	WB, ELISA, FACS, ICC, Cell-based IHC, BLI/SPR affinity assay, Biacore™ SPR assay, etc.

Note: This customized solution applies to all types of proteins, antibody species, isotypes, and fragments.
1. All incoming projects are subjected to evaluation.
2. Optional steps are offered with an extra charge upon request.
3. The 10 business days is decided from sequence evaluation.

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Case Studies

High expression titers of multiple isotypes.
High purity of antibodies expressed in TurboCHO™ Express system

Bi-specific Antibody Production – Case 1, Titer: 374 mg/L with Purity > 99%

Bi-specific Antibody Production – Case 2, After purification yield: 65mg/L, Purity (SDS-Page)>95%, Purity (SEC)>93%

Bi-specific Antibody Production – Case 3, After purification yield: 98mg/L, Purity (SDS-Page)>90%, Purity (SEC)>99%

FucoFree TurboCHO™ Express

1. Similar expression performance in Fuco free TurboCHO system compared to wild-type TurboCHO!

No.	Control Titer (mg/L)	Fuco-free Titer (mg/L)	Titer Differences (%)
test-1	574	577	0.50%
test-2	575	612	6.07%
test-3	577	565	-2.08%

2. Comparable purity in Fuco free TurboCHO system compared to wild-type TurboCHO!

3. No fucosylation modification in Fuco free TurboCHO system compared to wild-type TurboCHO!

N-Glycan	G0F-GlcNAc	Man 5	G0F	G1F
TurboCHO™	15.5%	10.7%	65.3%	8.5%
N-Glycan	G0-GlcNAc	Man 5	G0	G1
FucoFree TurboCHO™	15.0%	8.9%	71.2%	4.9%

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Quotation and Ordering

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