Titer Data of TurboCHO™ Stable Cell Pool & Cell Line
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![TurboCHO™ Recombinant Expression Service]( "TurboCHO™ Recombinant Expression Service")
Overview
Stable CHO-K1 cell pools/lines are commonly created using recombinant DNA technology, where a foreign gene is introduced into the cells, and selective pressure is applied to enrich for cells that have incorporated and stably express the foreign gene. This approach offers several advantages over transient expression systems, including consistent protein supply, reduced variability between batches, and scalability to industrial levels. However, some next-generation biotherapeutics are difficult to express at desired quantities in CHO cells, which led to the establishment of the TurboCHO™ stable cell pool/cell line expression platform by GenScript. This platform, coupled with AI-powered GenSmart™ codon optimization system and optimized fed-batch culture process, provides high expression levels for high-quality recombinant protein/antibody with fast turnaround times. The expression results are comparable with the TurboCHO™ transient expression platform (TurboCHO™ HT & TurboCHO™ Express), offering more flexible choices from small to large scale during the discovery timeline.
Highlights
High Expression Level
Average expression level (Fc-related antibody): Cell pool: 1.6~2.0 g/L; Cell line: 2.7~3.0 g/L
License-Agreed Cell
ECACC-licensed CHO-K1 cell
Flexible payments based on milestones
High-Quality Products
High purity antibodies
Customized QC tests
Service Details
Service Details Timeline Deliverables Phase I: Plasmid & Cell line preparation Codon optimization & plasmid construction 2 wks Top clones/pools (2 vials, 1×107 cells/ml, 1mL/vial) Stable cell pool generation (including transfection, screening, selection) 8 wks Stable cell line generation (including transfection, screening, single clone selection and cryopreservation) (optional) 4-6 wks Fed-batch culture and expression titer (for IgG-like antibodies and Fc-fusion proteins) determination, QC for cell pool/line 2 wks Report with mycoplasma test and expression titer Phase II: Scale-up expression and purification 1L fed-batch cell expression & one-step purification 2-3 wks - Purified antibody
- CoA Report (SEC-HPLC, endotoxin)
Option: N liter fed-batch cell expression & one-step purification Depends on scale - Purified antibody
- CoA Report (SEC-HPLC, endotoxin)
Note:
1. GenScript will cryopreserve 2 vials of the top stable cell line for further scaling-up (excluding the cell stocks to be delivered) for 12 months with no extra charge. GenScript offers an optional extension package of storage service for frozen cell stocks.
2. Developed cell lines and proteins produced by corresponding cells in this proposal are limited to Research Use. A new license agreement is needed if the client would like to use the developed cell line and produced proteins for commercial purposes.Case Studies
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High Expression Level of Antibodies Expressed with
TurboCHO™ Stable Cell Pool & Cell Line Platform
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High Purity of Antibodies Expressed with
TurboCHO™ Stable Cell Pool & Cell Line Platform
Human IgG1 Antibody Production, Expression Titer: 1749 mg/L; Purity (SDS-Page): >95%; Purity (SEC-HPLC): >99%
Rabbit IgG Antibody Production, Final Yield: 1380 mg/L; Purity (SDS-Page): >95%; Purity (SEC-HPLC): >99%
mIgG2a-scFv Antibody Production, Final Yield: 882 mg/L; Purity (SDS-Page): >95%; Purity (SEC-HPLC): >99%
Recombinant His-Protein Production, Final Yield: 493 mg/L; Purity (SDS-Page): >90%; Purity (SEC-HPLC): >93%
Related Resources
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Webinars
A Novel and Rapid Platform to Generate Gram-level Antibodies for Diagnostic and Therapeutic Use
The importance of protein expression in antibody drug discovery.
The progress of CHO cell line development.
How GenScript TurboCHO platform provides protein/antibody expression services for antibody drug discovery solutions.
Learn MoreTherapeutic Recombinant Antibody Development with Next Generation Proprietary CHO Expression SystemOverview of recombinant therapeutic antibody
Host selection for recombinant antibody expression
Why CHO expression system is still the favorable workhorse for recombinant protein production
Learn More
Additional Services
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with GenScript Custom
Protein Services
TurboCHO™ Antibody Expression in Singapore
From gene synthesis to antibody delivery starting at 8 BDs
Quotation and Ordering
Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.
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