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Customized Stable Cell Line Development

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Cell Engineering

Overview

Cellular assays, or stable cell-based assays, can be used in both biomedical research and drug-discovery screening applications to efficiently quantify cytotoxicity, biological activity, biochemical mechanisms and off-target interactions. The advantages of cell-based assays include the facilitation and generation of complex and biologically relevant data. Unlike traditional biochemical assays, cell-based assays are more physiologically relevant and can assess compound characteristics simultaneously.

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250+ cells delivery

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Extensive proteins experience

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PhD Experts support

Workflow

Assay Cell Line Workflow

Service Specifications

Service Name Service Detail Service Method Deliverables Delivery Time
CellPower™ Gene Constitutive Expression
  • Single gene overexpression stable cell pool/cell line
  • Multiple gene overexpression table cell pool/cell line
Viral Based
  • 2 cryovials of stable cell pool with 10^6 cells per vial
  • 4 cryovials of single-cell-derived stable clones with 10^6 cells per vial (2 clones)
  • Cell pool generation report
  • Cell clones screening report
  • CoA report
  • Starting from 8 weeks (Cell Pool)
  • Starting from 14 weeks (Cell clone)
Chemical Based
CellPower™ Gene Inducible Expression
  • Tet-On system
  • Tet-Off system
Viral Based
  • 2 cryovials of stable cell pool with 10^6 cells per vial
  • 2 cryovials of single-cell-derived stable clones with 10^6 cells per vial (1 clones)
  • Cell pool generation report
  • Cell clones screening report
  • CoA report
  • Starting from 12 weeks (Cell Pool)
  • Starting from 18 weeks (Cell clone)
Chemical Based
For knockdown cell line, please contact us for further detail.

It is preferred that customers provide their own cell lines, despite available cell lines can be purchased by GenScript for an additional fee. Please note that we currently do not provide genome editing service for primary cells, stem cells or iPS cells.
Validation services for Assay cell lines can be found under the "Add On Services" tab.

Add-On Service

Add-On Service Description
Reverse transcription (RT)-PCR Validate the positive cell pool/clones at mRNA level
FACS analysis Validate the transgene expression by FACS analysis. A validated antibody with specific binding to target protein or reporter tag must be provided.
Western Blot Validate the positive clones by western blot. A validated antibody in the host cells with specific binding to target protein must be provided.
Expression stability test Validate the GOI expression stability by WB or FACS in 15/20 generation.
One additional clone QC and deliver one additional positive clone.

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