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Lentiviral vectors, derived from the human immunodeficiency virus type 1 (HIV-1), are powerful and efficient gene delivery tools. They are capable of efficiently transducing both dividing and non-dividing cells, such as neurons and stem cells. By integrating target gene into the host genome, they enable stable and long-term target gene expression—making them essential in gene therapy, cell therapy, and vaccine development.
GenScript utilizes a third-generation self-inactivating (SIN) lentiviral system to provide a one-stop lentivirus packaging service—from vector design to virus packaging and purification. Our advanced serum-free suspension culture technology, combined with optimized vector GLV3, guarantees high-titer lentivirus production up to 109 TU/mL. GenScript proprietary lentivirus packaging platform delivers high-quality lentivirus with industry-leading turnaround times—just 5 business days from plasmid to lentivirus.
5 BDs
to Lentivirus Packaging
10 BDs from gene to lentivirus
1E+8 TU/mL
Guaranteed Functional Titer
Average delivered titer 3 times higher than guaranteed
>10Kb Large Insert
Maximum 400% Titer Boost
Proprietary lentivirus platform for large insert with unmatched efficiency
100% Accuracy
Functional Titer
Exclusive Default QC - Duplex qPCR
| Scale | Guaranteed Functional Titer ⓘ | Volume | TAT | Default QC | Deliverable |
|---|---|---|---|---|---|
| Purified | > 1E+8 TU/mL | 0.1 mL |
Starting at 7 business days (Duplex qPCR) new Starting at 5 business days (P24 ELISA) |
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| 1 mL | |||||
| 5 mL | |||||
| > 1E+9 TU/mL | 1mL | ||||
| Supernatant | > 2E+6 TU/mL | 0.2 mL | Starting at 5 business days |
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Note:
P24 ELISA: Quantify capsid proteins, measures the total number of viral particles (physical titer). The calculated functional titer (TU/mL) is derived from the P24 titer equation and represents a predicted value, which may differ from actual functional titers.
Duplex qPCR: Directly measure and quantify the infectious viral particles capable of transducing target cells, providing precise functional titer.
| Application | Vector Name | Insert Capacity | Promoter | Linker | Antibiotics Marker | Fluorescence Marker |
|---|---|---|---|---|---|---|
| Constitutive Overexpression | GLV3-CAG-[ORF]-PGK-BSD | 4853 | CAG | / | BSD | / |
| GLV3-CAG-[ORF]-PGK-Puro | 4652 | CAG | / | Puro | / | |
| GLV3-CAG-[ORF]-PGK-BSD-P2A-EGFP | 4070 | CAG | P2A | BSD | EGFP | |
| GLV3-CAG-[ORF]-PGK-BSD-P2A-mCherry | 4079 | CAG | P2A | BSD | mCherry | |
| GLV3-CAG-[ORF]-PGK-Puro-P2A-EGFP | 3869 | CAG | P2A | Puro | EGFP | |
| GLV3-CAG-[ORF]-PGK-Puro-P2A-mCherry | 3878 | CAG | P2A | Puro | mCherry | |
| GLV3-CMV-[ORF]-PGK-BSD | 4861 | CMV | / | BSD | / | |
| GLV3-CMV-[ORF]-PGK-Hyg | 4234 | CMV | / | Hygro | / | |
| GLV3-CMV-[ORF]-PGK-Neo | 4465 | CMV | / | Neo | / | |
| GLV3-CMV-[ORF]-PGK-Puro | 4660 | CMV | / | Puro | / | |
| GLV3-CMV-[ORF]-hPGK-EGFP-P2A-BleoR-WPRE | 3803 | CMV | P2A | BleoR | EGFP | |
| GLV3-CMV-[ORF]-hPGK-mCherry-P2A-BleoR-WPRE | 3812 | CMV | P2A | BleoR | mCherry | |
| GLV3-CMV-[ORF]-PGK-BSD-P2A-EGFP | 3979 | CMV | P2A | BSD | EGFP | |
| GLV3-CMV-[ORF]-PGK-BSD-P2A-mCherry | 3988 | CMV | P2A | BSD | mCherry | |
| GLV3-CMV-[ORF]-PGK-Puro-P2A-EGFP | 3778 | CMV | P2A | Puro | EGFP | |
| GLV3-CMV-[ORF]-PGK-Puro-P2A-mCherry | 3787 | CMV | P2A | Puro | mCherry | |
| GLV3-EF1a-[ORF]-PGK-BSD | 4266 | EF1a | / | BSD | / | |
| GLV3-EF1a-[ORF]-PGK-Hyg | 3639 | EF1a | / | Hygro | / | |
| GLV3-EF1a-[ORF]-PGK-Neo | 3870 | EF1a | / | Neo | / | |
| GLV3-EF1a-[ORF]-PGK-Puro | 4065 | EF1a | / | Puro | / | |
| GLV3-EF1a-[ORF]-PGK-BSD-P2A-EGFP | 3474 | EF1a | P2A | BSD | EGFP | |
| GLV3-EF1a-[ORF]-PGK-BSD-P2A-mCherry | 3483 | EF1a | P2A | BSD | mCherry | |
| GLV3-EF1a-[ORF]-PGK-Puro-P2A-EGFP | 3282 | EF1a | P2A | Puro | EGFP | |
| GLV3-EF1a-[ORF]-PGK-Puro-P2A-mCherry | 3282 | EF1a | P2A | Puro | mCherry | |
| GLV3-EF1α-[ORF]-hPGK-EGFP-P2A-BleoR-WPRE | 3208 | EF1a | P2A | BleoR | EGFP | |
| GLV3-EF1α-[ORF]-hPGK-mCherry-P2A-BleoR-WPRE | 3217 | EF1a | P2A | BleoR | mCherry | |
| Inducible Expression | GLV3-TRE tight-EGFP-EFS-rtTA-ad-PGK-Puro | 3935 | TRE tight | / | Puro | / |
| GLV3-TRE tight-EGFP-EFS-tTA-ad-PGK-Puro | 3935 | TRE tight | / | Puro | / | |
| CRISPR System | GLV3-U6-sgRNA(Sp)-hPGK-BleoR-WPRE | 4952 | U6 | / | BleoR | / |
| GLV3-U6-sgRNA(Sp)-hPGK-PuroR-WPRE | 4727 | U6 | / | PuroR | / | |
| GLV3-U6-sgRNA(Sp)-hPGK-BSD-WPRE | 4928 | U6 | / | BSD | / | |
| GLV3-U6-sgRNA(Sp)-hPGK-EGFP-WPRE | 4607 | U6 | / | / | EGFP | |
| GLV3-U6-sgRNA(Sp)-hPGK-EGFP-P2A-BleoR-WPRE | 4169 | U6 | P2A | BleoR | EGFP | |
| GLV3-U6-sgRNA(Sp)-hPGK-EGFP-P2A-PuroR-WPRE | 3944 | U6 | P2A | PuroR | EGFP | |
| GLV3-U6-sgRNA(Sp)-hPGK-EGFP-P2A-BSD-WPRE | 4145 | U6 | P2A | BSD | EGFP | |
| GLV3-U6-sgRNA(Sp)-hPGK-mCherry-WPRE | 4616 | U6 | / | / | mCherry | |
| GLV3-U6-sgRNA(Sp)-hPGK-mCherry-P2A-BleoR-WPRE | 4178 | U6 | P2A | BleoR | mCherry | |
| GLV3-U6-sgRNA(Sp)-hPGK-mCherry-P2A-PuroR-WPRE | 3953 | U6 | P2A | PuroR | mCherry | |
| GLV3-U6-sgRNA(Sp)-hPGK-mCherry-P2A-BSD-WPRE | 4154 | U6 | P2A | BSD | mCherry | |
| GLV3-EFS-SpCas9-hPGK-BleoR-WPRE | / | EFS | / | BleoR | / | |
| GLV3-EFS-SpCas9-hPGK-PuroR-WPRE | / | EFS | / | PuroR | / | |
| GLV3-EFS-SpCas9-hPGK-BSD-WPRE | / | EFS | / | BSD | / | |
| GLV3-U6-sgRNA(Sp)-EFS-SpCas9-P2A-BleoR-WPRE | 1016 | U6/EFS | P2A | BleoR | / | |
| GLV3-U6-sgRNA(Sp)-EFS-SpCas9-P2A-PuroR-WPRE | 791 | U6/EFS | P2A | PuroR | / | |
| GLV3-U6-sgRNA(Sp)-EFS-SpCas9-P2A-BSD-WPRE | 992 | U6/EFS | P2A | BSD | / | |
| GLV3-U6-sgRNA(Sp)-EFS-SpCas9-P2A-EGFP-WPRE | 671 | U6/EFS | P2A | / | EGFP | |
| GLV3-U6-sgRNA(Sp)-EFS-SpCas9-P2A-mCherry-WPRE | 680 | U6/EFS | P2A | / | mCherry |
| Bright Field | Fluorescence Field | |
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Positive control - 3 μL |
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Sample 1 - 1μL LTR Insert Length: >9 kb Duplex qPCR Titer: 2.52×10^8 TU/mL |
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Sample 2 - 1μL LTR Insert Length: >9 kb Duplex qPCR Titer: 2.06×10^8 TU/mL |
Challenge: The oversized insert (>10 kb) compromises lentiviral packaging efficiency, resulting in low viral titer and poor downstream performance.
Solution: Utilizing our proprietary lentivirus packaging system and GLV3 vector, we optimized each step of virus packaging specialized for large insert
(>6.5 kb) to achieve high-titer delivery.
Result:
| Bright Field | Fluorescence Field | |
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Positive Control - 3 μL Time: 48h after transduction |
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Sample 1- 3μL Duplex qPCR Titer: 1.03×10^8 TU/mL Time: 48h after transduction |
We can guarantee the minimum titer in most of the cases except the following situations: