SMCR8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMCR8
Entrez Gene ID	140775
Full Name	Smith-Magenis syndrome chromosome region, candidate 8
General protein information	Preferred Names Smith-Magenis syndrome chromosome region, candidate 8 Names guanine nucleotide exchange protein SMCR8 smith-Magenis syndrome chromosomal region candidate gene 8 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p11.2
Disorder MIM:	617074

mRNA and Protein(s)

mRNA	Protein	Name
NM_144775.2	NP_658988.2	guanine nucleotide exchange protein SMCR8
NM_144775.3	NP_658988.2	guanine nucleotide exchange protein SMCR8

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMCR8	XP_851653.1
Rattus norvegicus (Norway rat)	Smcr8	NP_001178848.1
Homo sapiens (human)	SMCR8	NP_658988.2
Bos taurus (cattle)	SMCR8	XP_003587459.2
Danio rerio (zebrafish)	smcr8a	XP_687034.3
Macaca mulatta (Rhesus monkey)	SMCR8	XP_001095753.1
Xenopus tropicalis (tropical clawed frog)	smcr8	XP_002935890.1
Gallus gallus (chicken)	SMCR8	XP_004945263.1
Pan troglodytes (chimpanzee)	SMCR8	XP_001157693.1
Mus musculus (house mouse)	Smcr8	NP_001078909.1

Gene Ontology
Bibliography

Related articles in PubMed

The C9orf72-interacting protein Smcr8 is a negative regulator of autoimmunity and lysosomal exocytosis.
Zhang Y, Burberry A, Wang JY, Sandoe J, Ghosh S, Udeshi ND, Svinkina T, Mordes DA, Mok J, Charlton M, Li QZ, Carr SA, Eggan K
Genes & development32(13-14)929-943(2018 07)

Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.
Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch AK, Müller S, Dikic I, Mittelbronn M, Behrends C
eLife6(2017 02)

C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling.
Amick J, Roczniak-Ferguson A, Ferguson SM
Molecular biology of the cell27(20)3040-3051(2016 10)

A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy.
Yang M, Liang C, Swaminathan K, Herrlinger S, Lai F, Shiekhattar R, Chen JF
Science advances2(9)e1601167(2016 09)

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.
Sullivan PM, Zhou X, Robins AM, Paushter DH, Kim D, Smolka MB, Hu F
Acta neuropathologica communications4(1)51(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMCR8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMCR8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31130	NM_144775.3 Latest version!	Homo sapiens SMCR8-C9orf72 complex subunit (SMCR8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
View Old Accession Versions >>
OHu31130	NM_144775.2	Homo sapiens Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMCR8 ( NM_144775.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_658988.2 Homo sapiens Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8), mRNA.

Price & Availability↑

CloneID	OHu31130
Clone ID Related Accession (Same CDS sequence)	NM_144775.2 , NM_144775.3
Accession Version	NM_144775.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514217600000
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange protein SMCR8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP280386.1, AF467440.1, BC101116.2, AC127537.8 and CK724925.1. On May 4, 2007 this sequence version replaced NM_144775.1. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.99884.1, BC142680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAGCG CCCCTGACGT AGTGGCCTTC ACCAAAGAGG AAGAGTATGA AGAAGAGCCT 
TACAATGAGC CGGCCCTGCC TGAGGAGTAC TCGGTGCCGC TCTTCCCCTT CGCCAGTCAG 
GGTGCTAACC CCTGGTCAAA ACTGTCCGGG GCCAAGTTTT CGAGGGACTT CATTCTTATT 
TCCGAGTTCT CTGAGCAGGT GGGACCCCAA CCCTTACTGA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT CAATTACTTC TCCCTGCGTA TCATGTCTGT GGATTACCAG 
GCTTCCTTCG TGGGCCATCC TCCTGGATCT GCCTACCCCA AGCTGAACTT CGTGGAGGAC 
TCCAAGGTGG TGCTGGGAGA TTCTAAGGAG GGCGCCTTTG CATACGTGCA CCACCTTACC 
CTATACGACC TGGAGGCCCG TGGCTTCGTG AGGCCGTTTT GCATGGCTTA TATCTCTGCA 
GACCAGCATA AAATCATGCA GCAGTTCCAG GAGCTTTCAG CCGAATTTTC CAGAGCTTCT 
GAGTGCTTGA AGACTGGCAA CAGGAAGGCA TTTGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACCAGGAC AGTGCTACAC ACAGAAACGG AGATCCAGAA GAAAGCCAAC 
GACAAAGGCT TTTACTCATC TCAGGCAATT GAGAAAGCCA ATGAACTGGC CAGTGTGGAG 
AAGTCCATCA TTGAACATCA AGACCTGCTG AAGCAGATCC GCTCATACCC TCATCGGAAG 
TTGAAGGGGC ATGATTTGTG TCCTGGTGAG ATGGAGCACA TCCAGGATCA GGCCAGCCAG 
GCATCCACTA CCTCTAACCC TGATGAGTCT GCCGACACAG ACCTTTACAC CTGCAGACCA 
GCCTACACCC CAAAACTTAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AGCTCTGTGA CACTGAATAT TTCACCCAGA CCCTGGCTCA GCTCAGCCAC 
ATTGAACACA TGTTCAGAGG AGACCTGTGT TACCTCCTGA CCAGTCAGAT TGATAGAGCA 
CTTCTAAAAC AACAGCATAT AACAAACTTT CTCTTTGAAG ACTTTGTGGA GGTCGATGAC 
AGGATGGTGG AGAAACAAGA AAGCATACCC TCTAAGCCCA GTCAAGACAG GCCGCCTTCC 
AGTTCTCTAG AAGAATGCCC AATTCCTAAA GTGTTAATTA GTGTTGGTTC TTACAAGTCC 
AGTGTGGAGT CTGTGTTGAT CAAGATGGAG CAGGAACTGG GAGATGAGGA GTACAAGGAA 
GTGGAAGTGA CTGAGTTGAG CAGTTTCGAC CCCCAGGAAA ACTTGGACTA CCTGGATATG 
GATATGAAAG GGAGTATCAG CAGTGGTGAA AGTATTGAAG TTTTGGGCAC GGAGAAATCC 
ACCTCCGTGC TTTCTAAATC TGACAGCCAG GCAAGCCTCA CAGTACCATT GAGCCCCCAG 
GTGGTCCGGA GCAAAGCAGT CAGCCACAGG ACCATCAGCG AGGACAGTAT TGAAGTCCTC 
AGTACCTGCC CCTCTGAGGC CCTCATCCCT GATGACTTTA AGGCCAGCTA CCCAAGTGCC 
ATTAATGAAG AAGAATCATA TCCAGATGGC AATGAAGGAG CCATCCGCTT CCAGGCAAGC 
ATCAGTCCTC CAGAACTGGG TGAGACAGAG GAAGGCAGCA TAGAAAACAC CCCATCACAA 
ATAGACTCCT CCTGCTGTAT TGGGAAGGAG AGCGATGGTC AGTTGGTGCT GCCCTCCACT 
CCAGCCCACA CACACTCTGA CGAGGATGGG GTGGTGAGCA GCCCCCCACA GCGCCACAGG 
CAGAAGGACC AGGGGTTCCG TGTAGACTTT TCAGTGGAAA ATGCCAACCC TTCTTCCCGA 
GACAACAGTT GTGAAGGGTT TCCCGCTTAT GAGCTGGACC CGAGCCACCT GCTGGCTAGC 
CGGGACATCA GTAAGACCAG CCTGGACAAC TACTCAGACA CCACCAGCTA CGTGAGCAGT 
GTAGCGTCCA CCAGCTCAGA CAGGATCCCC TCTGCTTATC CTGCTGGCCT GTCTTCCGAT 
AGGCATAAAA AGAGGGCTGG CCAGAACGCC TTAAAATTCA TCCGCCAGTA CCCCTTTGCC 
CACCCAGCCA TCTACTCCCT GCTCAGTGGG AGGACACTTG TGGTCCTGGG GGAAGATGAG 
GCCATAGTCA GGAAACTCGT GACTGCACTG GCTATCTTTG TCCCCAGCTA TGGCTGCTAC 
GCTAAGCCCG TGAAACATTG GGCCTCCTCC CCTTTGCACA TTATGGATTT TCAGAAGTGG 
AAGCTTATTG GCTTGCAGAG AGTGGCCTCC CCTGCCGGTG CCGGTACCCT CCATGCCCTG 
AGCCGCTACA GCCGCTACAC GAGCATCCTG GACCTTGACA ACAAAACCCT GCGCTGCCCC 
CTTTACAGAG GCACCCTGGT GCCCCGCCTG GCAGACCACC GCACACAGAT CAAGCGGGGC 
AGCACCTACT ACCTGCATGT CCAGAGCATG CTCACCCAGC TCTGCTCCAA GGCCTTCCTC 
TACACCTTCT GCCACCACCT GCACCTGCCT ACCCACGACA AGGAGACAGA GGAGCTGGTA 
GCCAGCCGCC AGATGAGCTT CCTAAAGCTG ACCCTGGGTC TGGTGAATGA GGATGTTAGG 
GTGGTCCAGT ACCTGGCTGA GCTGCTGAAG CTGCACTACA TGCAGGAATC TCCAGGGACC 
AGCCACCCCA TGCTCAGGTT TGACTATGTC CCCAGCTTTT TGTATAAAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_658988.2
CDS	511..3324
Translation	MISAPDVVAFTKEEEYEEEPYNEPALPEEYSVPLFPFASQGANPWSKLSGAKFSRDFILISEFSEQVGPQPLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSRASECLKTGNRKAFAGELEKKLKDLDYTRTVLHTETEIQKKANDKGFYSSQAIEKANELASVEKSIIEHQDLLKQIRSYPHRKLKGHDLCPGEMEHIQDQASQASTTSNPDESADTDLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGDLCYLLTSQIDRALLKQQHITNFLFEDFVEVDDRMVEKQESIPSKPSQDRPPSSSLEECPIPKVLISVGSYKSSVESVLIKMEQELGDEEYKEVEVTELSSFDPQENLDYLDMDMKGSISSGESIEVLGTEKSTSVLSKSDSQASLTVPLSPQVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEESYPDGNEGAIRFQASISPPELGETEEGSIENTPSQIDSSCCIGKESDGQLVLPSTPAHTHSDEDGVVSSPPQRHRQKDQGFRVDFSVENANPSSRDNSCEGFPAYELDPSHLLASRDISKTSLDNYSDTTSYVSSVASTSSDRIPSAYPAGLSSDRHKKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDEAIVRKLVTALAIFVPSYGCYAKPVKHWASSPLHIMDFQKWKLIGLQRVASPAGAGTLHALSRYSRYTSILDLDNKTLRCPLYRGTLVPRLADHRTQIKRGSTYYLHVQSMLTQLCSKAFLYTFCHHLHLPTHDKETEELVASRQMSFLKLTLGLVNEDVRVVQYLAELLKLHYMQESPGTSHPMLRFDYVPSFLYKI

Target ORF information:

RefSeq Version	NM_144775.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144775.2

ORF Insert Sequence:

ATGATCAGCG CCCCTGACGT AGTGGCCTTC ACCAAAGAGG AAGAGTATGA AGAAGAGCCT 
TACAATGAGC CGGCCCTGCC TGAGGAGTAC TCGGTGCCGC TCTTCCCCTT CGCCAGTCAG 
GGTGCTAACC CCTGGTCAAA ACTGTCCGGG GCCAAGTTTT CGAGGGACTT CATTCTTATT 
TCCGAGTTCT CTGAGCAGGT GGGACCCCAA CCCTTACTGA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT CAATTACTTC TCCCTGCGTA TCATGTCTGT GGATTACCAG 
GCTTCCTTCG TGGGCCATCC TCCTGGATCT GCCTACCCCA AGCTGAACTT CGTGGAGGAC 
TCCAAGGTGG TGCTGGGAGA TTCTAAGGAG GGCGCCTTTG CATACGTGCA CCACCTTACC 
CTATACGACC TGGAGGCCCG TGGCTTCGTG AGGCCGTTTT GCATGGCTTA TATCTCTGCA 
GACCAGCATA AAATCATGCA GCAGTTCCAG GAGCTTTCAG CCGAATTTTC CAGAGCTTCT 
GAGTGCTTGA AGACTGGCAA CAGGAAGGCA TTTGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACCAGGAC AGTGCTACAC ACAGAAACGG AGATCCAGAA GAAAGCCAAC 
GACAAAGGCT TTTACTCATC TCAGGCAATT GAGAAAGCCA ATGAACTGGC CAGTGTGGAG 
AAGTCCATCA TTGAACATCA AGACCTGCTG AAGCAGATCC GCTCATACCC TCATCGGAAG 
TTGAAGGGGC ATGATTTGTG TCCTGGTGAG ATGGAGCACA TCCAGGATCA GGCCAGCCAG 
GCATCCACTA CCTCTAACCC TGATGAGTCT GCCGACACAG ACCTTTACAC CTGCAGACCA 
GCCTACACCC CAAAACTTAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AGCTCTGTGA CACTGAATAT TTCACCCAGA CCCTGGCTCA GCTCAGCCAC 
ATTGAACACA TGTTCAGAGG AGACCTGTGT TACCTCCTGA CCAGTCAGAT TGATAGAGCA 
CTTCTAAAAC AACAGCATAT AACAAACTTT CTCTTTGAAG ACTTTGTGGA GGTCGATGAC 
AGGATGGTGG AGAAACAAGA AAGCATACCC TCTAAGCCCA GTCAAGACAG GCCGCCTTCC 
AGTTCTCTAG AAGAATGCCC AATTCCTAAA GTGTTAATTA GTGTTGGTTC TTACAAGTCC 
AGTGTGGAGT CTGTGTTGAT CAAGATGGAG CAGGAACTGG GAGATGAGGA GTACAAGGAA 
GTGGAAGTGA CTGAGTTGAG CAGTTTCGAC CCCCAGGAAA ACTTGGACTA CCTGGATATG 
GATATGAAAG GGAGTATCAG CAGTGGTGAA AGTATTGAAG TTTTGGGCAC GGAGAAATCC 
ACCTCCGTGC TTTCTAAATC TGACAGCCAG GCAAGCCTCA CAGTACCATT GAGCCCCCAG 
GTGGTCCGGA GCAAAGCAGT CAGCCACAGG ACCATCAGCG AGGACAGTAT TGAAGTCCTC 
AGTACCTGCC CCTCTGAGGC CCTCATCCCT GATGACTTTA AGGCCAGCTA CCCAAGTGCC 
ATTAATGAAG AAGAATCATA TCCAGATGGC AATGAAGGAG CCATCCGCTT CCAGGCAAGC 
ATCAGTCCTC CAGAACTGGG TGAGACAGAG GAAGGCAGCA TAGAAAACAC CCCATCACAA 
ATAGACTCCT CCTGCTGTAT TGGGAAGGAG AGCGATGGTC AGTTGGTGCT GCCCTCCACT 
CCAGCCCACA CACACTCTGA CGAGGATGGG GTGGTGAGCA GCCCCCCACA GCGCCACAGG 
CAGAAGGACC AGGGGTTCCG TGTAGACTTT TCAGTGGAAA ATGCCAACCC TTCTTCCCGA 
GACAACAGTT GTGAAGGGTT TCCCGCTTAT GAGCTGGACC CGAGCCACCT GCTGGCTAGC 
CGGGACATCA GTAAGACCAG CCTGGACAAC TACTCAGACA CCACCAGCTA CGTGAGCAGT 
GTAGCGTCCA CCAGCTCAGA CAGGATCCCC TCTGCTTATC CTGCTGGCCT GTCTTCCGAT 
AGGCATAAAA AGAGGGCTGG CCAGAACGCC TTAAAATTCA TCCGCCAGTA CCCCTTTGCC 
CACCCAGCCA TCTACTCCCT GCTCAGTGGG AGGACACTTG TGGTCCTGGG GGAAGATGAG 
GCCATAGTCA GGAAACTCGT GACTGCACTG GCTATCTTTG TCCCCAGCTA TGGCTGCTAC 
GCTAAGCCCG TGAAACATTG GGCCTCCTCC CCTTTGCACA TTATGGATTT TCAGAAGTGG 
AAGCTTATTG GCTTGCAGAG AGTGGCCTCC CCTGCCGGTG CCGGTACCCT CCATGCCCTG 
AGCCGCTACA GCCGCTACAC GAGCATCCTG GACCTTGACA ACAAAACCCT GCGCTGCCCC 
CTTTACAGAG GCACCCTGGT GCCCCGCCTG GCAGACCACC GCACACAGAT CAAGCGGGGC 
AGCACCTACT ACCTGCATGT CCAGAGCATG CTCACCCAGC TCTGCTCCAA GGCCTTCCTC 
TACACCTTCT GCCACCACCT GCACCTGCCT ACCCACGACA AGGAGACAGA GGAGCTGGTA 
GCCAGCCGCC AGATGAGCTT CCTAAAGCTG ACCCTGGGTC TGGTGAATGA GGATGTTAGG 
GTGGTCCAGT ACCTGGCTGA GCTGCTGAAG CTGCACTACA TGCAGGAATC TCCAGGGACC 
AGCCACCCCA TGCTCAGGTT TGACTATGTC CCCAGCTTTT TGTATAAAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCR8 ( NM_144775.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_658988.2 Homo sapiens SMCR8-C9orf72 complex subunit (SMCR8), mRNA.

Price & Availability↑

CloneID	OHu31130
Clone ID Related Accession (Same CDS sequence)	NM_144775.2 , NM_144775.3
Accession Version	NM_144775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559923200000
Organism	Homo sapiens(human)
Product	guanine nucleotide exchange protein SMCR8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP280386.1, AF467440.1, BC101116.2, AC127537.8 and CK724925.1. On Nov 22, 2018 this sequence version replaced NM_144775.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.99884.1, BC142680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000406438.5/ ENSP00000385025.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATCAGCG CCCCTGACGT AGTGGCCTTC ACCAAAGAGG AAGAGTATGA AGAAGAGCCT 
TACAATGAGC CGGCCCTGCC TGAGGAGTAC TCGGTGCCGC TCTTCCCCTT CGCCAGTCAG 
GGTGCTAACC CCTGGTCAAA ACTGTCCGGG GCCAAGTTTT CGAGGGACTT CATTCTTATT 
TCCGAGTTCT CTGAGCAGGT GGGACCCCAA CCCTTACTGA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT CAATTACTTC TCCCTGCGTA TCATGTCTGT GGATTACCAG 
GCTTCCTTCG TGGGCCATCC TCCTGGATCT GCCTACCCCA AGCTGAACTT CGTGGAGGAC 
TCCAAGGTGG TGCTGGGAGA TTCTAAGGAG GGCGCCTTTG CATACGTGCA CCACCTTACC 
CTATACGACC TGGAGGCCCG TGGCTTCGTG AGGCCGTTTT GCATGGCTTA TATCTCTGCA 
GACCAGCATA AAATCATGCA GCAGTTCCAG GAGCTTTCAG CCGAATTTTC CAGAGCTTCT 
GAGTGCTTGA AGACTGGCAA CAGGAAGGCA TTTGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACCAGGAC AGTGCTACAC ACAGAAACGG AGATCCAGAA GAAAGCCAAC 
GACAAAGGCT TTTACTCATC TCAGGCAATT GAGAAAGCCA ATGAACTGGC CAGTGTGGAG 
AAGTCCATCA TTGAACATCA AGACCTGCTG AAGCAGATCC GCTCATACCC TCATCGGAAG 
TTGAAGGGGC ATGATTTGTG TCCTGGTGAG ATGGAGCACA TCCAGGATCA GGCCAGCCAG 
GCATCCACTA CCTCTAACCC TGATGAGTCT GCCGACACAG ACCTTTACAC CTGCAGACCA 
GCCTACACCC CAAAACTTAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AGCTCTGTGA CACTGAATAT TTCACCCAGA CCCTGGCTCA GCTCAGCCAC 
ATTGAACACA TGTTCAGAGG AGACCTGTGT TACCTCCTGA CCAGTCAGAT TGATAGAGCA 
CTTCTAAAAC AACAGCATAT AACAAACTTT CTCTTTGAAG ACTTTGTGGA GGTCGATGAC 
AGGATGGTGG AGAAACAAGA AAGCATACCC TCTAAGCCCA GTCAAGACAG GCCGCCTTCC 
AGTTCTCTAG AAGAATGCCC AATTCCTAAA GTGTTAATTA GTGTTGGTTC TTACAAGTCC 
AGTGTGGAGT CTGTGTTGAT CAAGATGGAG CAGGAACTGG GAGATGAGGA GTACAAGGAA 
GTGGAAGTGA CTGAGTTGAG CAGTTTCGAC CCCCAGGAAA ACTTGGACTA CCTGGATATG 
GATATGAAAG GGAGTATCAG CAGTGGTGAA AGTATTGAAG TTTTGGGCAC GGAGAAATCC 
ACCTCCGTGC TTTCTAAATC TGACAGCCAG GCAAGCCTCA CAGTACCATT GAGCCCCCAG 
GTGGTCCGGA GCAAAGCAGT CAGCCACAGG ACCATCAGCG AGGACAGTAT TGAAGTCCTC 
AGTACCTGCC CCTCTGAGGC CCTCATCCCT GATGACTTTA AGGCCAGCTA CCCAAGTGCC 
ATTAATGAAG AAGAATCATA TCCAGATGGC AATGAAGGAG CCATCCGCTT CCAGGCAAGC 
ATCAGTCCTC CAGAACTGGG TGAGACAGAG GAAGGCAGCA TAGAAAACAC CCCATCACAA 
ATAGACTCCT CCTGCTGTAT TGGGAAGGAG AGCGATGGTC AGTTGGTGCT GCCCTCCACT 
CCAGCCCACA CACACTCTGA CGAGGATGGG GTGGTGAGCA GCCCCCCACA GCGCCACAGG 
CAGAAGGACC AGGGGTTCCG TGTAGACTTT TCAGTGGAAA ATGCCAACCC TTCTTCCCGA 
GACAACAGTT GTGAAGGGTT TCCCGCTTAT GAGCTGGACC CGAGCCACCT GCTGGCTAGC 
CGGGACATCA GTAAGACCAG CCTGGACAAC TACTCAGACA CCACCAGCTA CGTGAGCAGT 
GTAGCGTCCA CCAGCTCAGA CAGGATCCCC TCTGCTTATC CTGCTGGCCT GTCTTCCGAT 
AGGCATAAAA AGAGGGCTGG CCAGAACGCC TTAAAATTCA TCCGCCAGTA CCCCTTTGCC 
CACCCAGCCA TCTACTCCCT GCTCAGTGGG AGGACACTTG TGGTCCTGGG GGAAGATGAG 
GCCATAGTCA GGAAACTCGT GACTGCACTG GCTATCTTTG TCCCCAGCTA TGGCTGCTAC 
GCTAAGCCCG TGAAACATTG GGCCTCCTCC CCTTTGCACA TTATGGATTT TCAGAAGTGG 
AAGCTTATTG GCTTGCAGAG AGTGGCCTCC CCTGCCGGTG CCGGTACCCT CCATGCCCTG 
AGCCGCTACA GCCGCTACAC GAGCATCCTG GACCTTGACA ACAAAACCCT GCGCTGCCCC 
CTTTACAGAG GCACCCTGGT GCCCCGCCTG GCAGACCACC GCACACAGAT CAAGCGGGGC 
AGCACCTACT ACCTGCATGT CCAGAGCATG CTCACCCAGC TCTGCTCCAA GGCCTTCCTC 
TACACCTTCT GCCACCACCT GCACCTGCCT ACCCACGACA AGGAGACAGA GGAGCTGGTA 
GCCAGCCGCC AGATGAGCTT CCTAAAGCTG ACCCTGGGTC TGGTGAATGA GGATGTTAGG 
GTGGTCCAGT ACCTGGCTGA GCTGCTGAAG CTGCACTACA TGCAGGAATC TCCAGGGACC 
AGCCACCCCA TGCTCAGGTT TGACTATGTC CCCAGCTTTT TGTATAAAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_658988.2
CDS	498..3311
Translation	MISAPDVVAFTKEEEYEEEPYNEPALPEEYSVPLFPFASQGANPWSKLSGAKFSRDFILISEFSEQVGPQPLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSRASECLKTGNRKAFAGELEKKLKDLDYTRTVLHTETEIQKKANDKGFYSSQAIEKANELASVEKSIIEHQDLLKQIRSYPHRKLKGHDLCPGEMEHIQDQASQASTTSNPDESADTDLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGDLCYLLTSQIDRALLKQQHITNFLFEDFVEVDDRMVEKQESIPSKPSQDRPPSSSLEECPIPKVLISVGSYKSSVESVLIKMEQELGDEEYKEVEVTELSSFDPQENLDYLDMDMKGSISSGESIEVLGTEKSTSVLSKSDSQASLTVPLSPQVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEESYPDGNEGAIRFQASISPPELGETEEGSIENTPSQIDSSCCIGKESDGQLVLPSTPAHTHSDEDGVVSSPPQRHRQKDQGFRVDFSVENANPSSRDNSCEGFPAYELDPSHLLASRDISKTSLDNYSDTTSYVSSVASTSSDRIPSAYPAGLSSDRHKKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDEAIVRKLVTALAIFVPSYGCYAKPVKHWASSPLHIMDFQKWKLIGLQRVASPAGAGTLHALSRYSRYTSILDLDNKTLRCPLYRGTLVPRLADHRTQIKRGSTYYLHVQSMLTQLCSKAFLYTFCHHLHLPTHDKETEELVASRQMSFLKLTLGLVNEDVRVVQYLAELLKLHYMQESPGTSHPMLRFDYVPSFLYKI

Target ORF information:

RefSeq Version	NM_144775.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMCR8-C9orf72 complex subunit (SMCR8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144775.3

ORF Insert Sequence:

ATGATCAGCG CCCCTGACGT AGTGGCCTTC ACCAAAGAGG AAGAGTATGA AGAAGAGCCT 
TACAATGAGC CGGCCCTGCC TGAGGAGTAC TCGGTGCCGC TCTTCCCCTT CGCCAGTCAG 
GGTGCTAACC CCTGGTCAAA ACTGTCCGGG GCCAAGTTTT CGAGGGACTT CATTCTTATT 
TCCGAGTTCT CTGAGCAGGT GGGACCCCAA CCCTTACTGA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT CAATTACTTC TCCCTGCGTA TCATGTCTGT GGATTACCAG 
GCTTCCTTCG TGGGCCATCC TCCTGGATCT GCCTACCCCA AGCTGAACTT CGTGGAGGAC 
TCCAAGGTGG TGCTGGGAGA TTCTAAGGAG GGCGCCTTTG CATACGTGCA CCACCTTACC 
CTATACGACC TGGAGGCCCG TGGCTTCGTG AGGCCGTTTT GCATGGCTTA TATCTCTGCA 
GACCAGCATA AAATCATGCA GCAGTTCCAG GAGCTTTCAG CCGAATTTTC CAGAGCTTCT 
GAGTGCTTGA AGACTGGCAA CAGGAAGGCA TTTGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACCAGGAC AGTGCTACAC ACAGAAACGG AGATCCAGAA GAAAGCCAAC 
GACAAAGGCT TTTACTCATC TCAGGCAATT GAGAAAGCCA ATGAACTGGC CAGTGTGGAG 
AAGTCCATCA TTGAACATCA AGACCTGCTG AAGCAGATCC GCTCATACCC TCATCGGAAG 
TTGAAGGGGC ATGATTTGTG TCCTGGTGAG ATGGAGCACA TCCAGGATCA GGCCAGCCAG 
GCATCCACTA CCTCTAACCC TGATGAGTCT GCCGACACAG ACCTTTACAC CTGCAGACCA 
GCCTACACCC CAAAACTTAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AGCTCTGTGA CACTGAATAT TTCACCCAGA CCCTGGCTCA GCTCAGCCAC 
ATTGAACACA TGTTCAGAGG AGACCTGTGT TACCTCCTGA CCAGTCAGAT TGATAGAGCA 
CTTCTAAAAC AACAGCATAT AACAAACTTT CTCTTTGAAG ACTTTGTGGA GGTCGATGAC 
AGGATGGTGG AGAAACAAGA AAGCATACCC TCTAAGCCCA GTCAAGACAG GCCGCCTTCC 
AGTTCTCTAG AAGAATGCCC AATTCCTAAA GTGTTAATTA GTGTTGGTTC TTACAAGTCC 
AGTGTGGAGT CTGTGTTGAT CAAGATGGAG CAGGAACTGG GAGATGAGGA GTACAAGGAA 
GTGGAAGTGA CTGAGTTGAG CAGTTTCGAC CCCCAGGAAA ACTTGGACTA CCTGGATATG 
GATATGAAAG GGAGTATCAG CAGTGGTGAA AGTATTGAAG TTTTGGGCAC GGAGAAATCC 
ACCTCCGTGC TTTCTAAATC TGACAGCCAG GCAAGCCTCA CAGTACCATT GAGCCCCCAG 
GTGGTCCGGA GCAAAGCAGT CAGCCACAGG ACCATCAGCG AGGACAGTAT TGAAGTCCTC 
AGTACCTGCC CCTCTGAGGC CCTCATCCCT GATGACTTTA AGGCCAGCTA CCCAAGTGCC 
ATTAATGAAG AAGAATCATA TCCAGATGGC AATGAAGGAG CCATCCGCTT CCAGGCAAGC 
ATCAGTCCTC CAGAACTGGG TGAGACAGAG GAAGGCAGCA TAGAAAACAC CCCATCACAA 
ATAGACTCCT CCTGCTGTAT TGGGAAGGAG AGCGATGGTC AGTTGGTGCT GCCCTCCACT 
CCAGCCCACA CACACTCTGA CGAGGATGGG GTGGTGAGCA GCCCCCCACA GCGCCACAGG 
CAGAAGGACC AGGGGTTCCG TGTAGACTTT TCAGTGGAAA ATGCCAACCC TTCTTCCCGA 
GACAACAGTT GTGAAGGGTT TCCCGCTTAT GAGCTGGACC CGAGCCACCT GCTGGCTAGC 
CGGGACATCA GTAAGACCAG CCTGGACAAC TACTCAGACA CCACCAGCTA CGTGAGCAGT 
GTAGCGTCCA CCAGCTCAGA CAGGATCCCC TCTGCTTATC CTGCTGGCCT GTCTTCCGAT 
AGGCATAAAA AGAGGGCTGG CCAGAACGCC TTAAAATTCA TCCGCCAGTA CCCCTTTGCC 
CACCCAGCCA TCTACTCCCT GCTCAGTGGG AGGACACTTG TGGTCCTGGG GGAAGATGAG 
GCCATAGTCA GGAAACTCGT GACTGCACTG GCTATCTTTG TCCCCAGCTA TGGCTGCTAC 
GCTAAGCCCG TGAAACATTG GGCCTCCTCC CCTTTGCACA TTATGGATTT TCAGAAGTGG 
AAGCTTATTG GCTTGCAGAG AGTGGCCTCC CCTGCCGGTG CCGGTACCCT CCATGCCCTG 
AGCCGCTACA GCCGCTACAC GAGCATCCTG GACCTTGACA ACAAAACCCT GCGCTGCCCC 
CTTTACAGAG GCACCCTGGT GCCCCGCCTG GCAGACCACC GCACACAGAT CAAGCGGGGC 
AGCACCTACT ACCTGCATGT CCAGAGCATG CTCACCCAGC TCTGCTCCAA GGCCTTCCTC 
TACACCTTCT GCCACCACCT GCACCTGCCT ACCCACGACA AGGAGACAGA GGAGCTGGTA 
GCCAGCCGCC AGATGAGCTT CCTAAAGCTG ACCCTGGGTC TGGTGAATGA GGATGTTAGG 
GTGGTCCAGT ACCTGGCTGA GCTGCTGAAG CTGCACTACA TGCAGGAATC TCCAGGGACC 
AGCCACCCCA TGCTCAGGTT TGACTATGTC CCCAGCTTTT TGTATAAAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The C9orf72-interacting protein Smcr8 is a negative regulator of autoimmunity and lysosomal exocytosis.
Genes & development32(13-14)929-943(2018 07)
Zhang Y,Burberry A,Wang JY,Sandoe J,Ghosh S,Udeshi ND,Svinkina T,Mordes DA,Mok J,Charlton M,Li QZ,Carr SA,Eggan K

Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.
eLife6(2017 02)
Jung J,Nayak A,Schaeffer V,Starzetz T,Kirsch AK,Müller S,Dikic I,Mittelbronn M,Behrends C

C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling.
Molecular biology of the cell27(20)3040-3051(2016 10)
Amick J,Roczniak-Ferguson A,Ferguson SM

A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy.
Science advances2(9)e1601167(2016 09)
Yang M,Liang C,Swaminathan K,Herrlinger S,Lai F,Shiekhattar R,Chen JF

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.
Acta neuropathologica communications4(1)51(2016 05)
Sullivan PM,Zhou X,Robins AM,Paushter DH,Kim D,Smolka MB,Hu F

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SMCR8 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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