Smcr8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smcr8
Entrez Gene ID	237782
Full Name	Smith-Magenis syndrome chromosome region, candidate 8 homolog (human)
Synonyms	2310076G09Rik,AI642055,D030073L15Rik
General protein information	Preferred Names Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) Names guanine nucleotide exchange protein SMCR8 smith-Magenis syndrome chromosomal region candidate gene 8 protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001085440.1	NP_001078909.1	guanine nucleotide exchange protein SMCR8 isoform 1
NM_175491.4	NP_780700.1	guanine nucleotide exchange protein SMCR8 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMCR8	XP_851653.1
Rattus norvegicus (Norway rat)	Smcr8	NP_001178848.1
Homo sapiens (human)	SMCR8	NP_658988.2
Bos taurus (cattle)	SMCR8	XP_003587459.2
Danio rerio (zebrafish)	smcr8a	XP_687034.3
Macaca mulatta (Rhesus monkey)	SMCR8	XP_001095753.1
Xenopus tropicalis (tropical clawed frog)	smcr8	XP_002935890.1
Gallus gallus (chicken)	SMCR8	XP_004945263.1
Pan troglodytes (chimpanzee)	SMCR8	XP_001157693.1
Mus musculus (house mouse)	Smcr8	NP_001078909.1

Gene Ontology
Bibliography

Related articles in PubMed

Excessive endosomal TLR signaling causes inflammatory disease in mice with defective SMCR8-WDR41-C9ORF72 complex function.
McAlpine W, Sun L, Wang KW, Liu A, Jain R, San Miguel M, Wang J, Zhang Z, Hayse B, McAlpine SG, Choi JH, Zhong X, Ludwig S, Russell J, Zhan X, Choi M, Li X, Tang M, Moresco EMY, Beutler B, Turer E
Proceedings of the National Academy of Sciences of the United States of America115(49)E11523-E11531(2018 12)

The C9orf72-interacting protein Smcr8 is a negative regulator of autoimmunity and lysosomal exocytosis.
Zhang Y, Burberry A, Wang JY, Sandoe J, Ghosh S, Udeshi ND, Svinkina T, Mordes DA, Mok J, Charlton M, Li QZ, Carr SA, Eggan K
Genes & development32(13-14)929-943(2018 07)

Loss of C9orf72 Enhances Autophagic Activity via Deregulated mTOR and TFEB Signaling.
Ugolino J, Ji YJ, Conchina K, Chu J, Nirujogi RS, Pandey A, Brady NR, Hamacher-Brady A, Wang J
PLoS genetics12(11)e1006443(2016 Nov)

A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy.
Yang M, Liang C, Swaminathan K, Herrlinger S, Lai F, Shiekhattar R, Chen JF
Science advances2(9)e1601167(2016 09)

Penetrance of craniofacial anomalies in mouse models of Smith-Magenis syndrome is modified by genomic sequence surrounding Rai1: not all null alleles are alike.
Yan J, Bi W, Lupski JR
American journal of human genetics80(3)518-25(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Smcr8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smcr8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20960	NM_001085440.1 Latest version!	Mus musculus Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) (Smcr8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu58383	NM_175491.4 Latest version!	Mus musculus Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) (Smcr8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smcr8 ( NM_001085440.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001078909.1 Mus musculus Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) (Smcr8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20960
Clone ID Related Accession (Same CDS sequence)	NM_001085440.1
Accession Version	NM_001085440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange protein SMCR8 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ099053.1, AK164045.1, BC085095.1, BC094221.1 and AW912487.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.167611.1, SRR1660817.164110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAGCG CCCCTGATGT GGTGGCCTTC ACCAAGGAAG ATGAATACGA GGAAGAACCT 
TACAATGAGC CCGCTTTGCC TGAGGAGTAC TCAGTCCCTC TCTTTCCTTA TGCCAGCCAG 
GGGGCAAACC CCTGGTCTAA ACTGTCTGGG GCCAAGTTCT CCAGGGACTT CATCCTCATT 
TCCGAGTTCT CTGAGCAGGT GGGACCCCAG CCCTTGCTTA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT TAATTACTTC TCTTTGCGGA TTATGTCGGT GGATTACCAG 
GCTTCATTCG TAGGCCATCC TCCCGGTTCT GCCTACCCCA AGTTGAACTT TGTGGAAGAC 
TCTAAAGTGG TACTGGGAGA CTCTAAGGAA GGGGCCTTTG CATATGTGCA CCACCTCACC 
TTGTACGACC TGGAGGCCAG GGGCTTTGTG AGGCCCTTTT GTATGGCTTA TATCTCCGCA 
GACCAGCATA AAATCATGCA GCAGTTCCAA GAGCTCTCGG CCGAATTTTC CAAAGCATCT 
GAGTGCTTGA AGATGGGCAA CAGGAAGGCA TTCGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACAAGGAC AGTGCTACAC ACAGAAACCG AGATCCAGAA GAAAGCCAAC 
GACAAGGGTT TTTATTCTTC TCAGGCGATT GAGAAAGCCA ATGAACTGGC CAATGTGGAG 
AAGTCCATCA TCGAACATCA AGATTTGCTG AGGCAGATCC GCTCATACCC TCGTCAGAAG 
ACGAAGATCC CTGACTTGCA GCCTGGTGAT ACAGAGCATA CCCAGGATCA GGCTGACCAG 
GTATCCACTA CCTCTAATCC TGAGGAGTCC GCTAATGCAG ACCTTTATAC CTGCAGACCG 
GCTTACACCC CCAAACTCAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AACTCTGTGA CACTGAGTAT TTCACTCAGA CCCTGGCCCA GCTTAGCCAC 
ATTGAACACA TGTTCAGAGG AGACCTGTGC TACCTCCTGA CCAGTCAGAT TGACAGAGTG 
CTTCGAAAAC AACAGCCCAT AACGAATTTC CTCTTTGAAG ATTTTGTAGA GGTGGATGAC 
AGGATGGAAA AGCAAGAGAA TGTACCCTCT CAGCCCAGTC AGGACAGGCT GCCTCCCAAG 
CCTGTAGAAG AATGCCCCAT TCCTAAAGTG TTAATTAGCG TTGGCTCTTA CAAGTCCAGT 
GTGGAGTCTG TATTGATCAA GATGGAGCAG GAACTTGGCG ATGAAGAGTA CACGGGGGTG 
GAGGCAACAG AGGCACGCAG TTTTGACCCC CAAGAGAACC TGGACTACCT GGATATGGAT 
ATGAAAGGGA GCATCAGTAG TGGGGAAAGC ATTGAGGTGC TGGGCACCGA GAAGTCAGCC 
TCTGTGTTGT CGAAATCTGA CAGCCAGGCC AGCCTCACCG TGCCATTGAG CCCCCATGTA 
GTCCGTAGCA AAGCGGTCAG CCACAGGACA ATCAGCGAGG ACAGCATTGA AGTCTTAAGT 
ACCTGCCCTT CTGAGGCCCT CATTCCTGAT GACTTTAAGG CCAGTTACCC AAGTGCCATT 
AATGAAGAAG AAGCCTATGC GGATAATGAG GGGGCCATCC ATTTCCAGGC AAGTGCCGGC 
TCACCAGAAC CGGATGAGAC TCAGGAGGGC AACTTGGAAA ATATCCCATC CCAAATAGAC 
TCCAGCTGCT GTATTGGAAA GGAGAGTGAA GGTCACTTGG TGCCTCTCCC AACCCCAGCC 
TACACTCTTT CTGATGAGGA CAGTGTGGTG AGCATTCCCC CACAGCGCTA CATACAGAAG 
GACCAGGGGC TCCACGTGGA CTTCGGAGTG GAAAACACTG ACCCTTCTCC CCGAGACAAC 
AGTTGTGAAA TGTTCCCAGC TTATGAGCTG GATCCAAGCT GCCTTCTGGC TAGCCGAGAT 
GTTAGTAAGA TGAGCCTGGA TAACTACTCG GATACCACTA GCTACATGGG CAGTGCGGCC 
TCTACCAGCT CAGACAGAAT CCCCTCAGCA CCTCCTGCTG GCCTATCCTC TGAGAGGCAC 
AAAAAGAGAG CTGGCCAGAA TGCCTTAAAA TTCATCCGCC AGTACCCTTT TGCCCACCCA 
GCCATCTACT CCCTGCTCAG TGGGAGGACA CTTGTGGTCC TGGGCGAAGA TGAAACCATT 
GTCAGGAAGC TGGTGACTGC ACTGTCCATC TTTGTTCCCA ACTATGGCTG CTATGCCAAG 
CCGGTGAAGC ACTGGATTTC TTCCCCTTTG CATATTATGG ATTTCCAGAA GTGGAAGCTT 
ATTGGCCTAC AAAGAGTGGC CTCTCCTGCC AATGTGGGTA CCCTCCATAC CCTGAGCCGT 
TACAGCCGTT ATACAAGCAT CCTGGACCTG GACAGCAAGA CCCTACGCTG TCCCCTATAC 
AGGGGTACAC TGGTACCCCG CCTGGCTGAC CATCGCACTC AGATCAAGCG GGGCAGCACA 
TACTACCTTC ACGTCCAGAG CATGCTCACC CAGCTCTGCT CCAAGGCATT CCTCTACACC 
TTTTGTCACC ACTTGCATCT GCCCGCCCAC AGTGAGGAGA CACAAGAAGC AGTGGCCAGC 
AGACAAACCA GCTTCCTAAA GCTAAACCTG GGGCTCGTGA ACGAGGACAT CAGAGTGGTA 
CAGTACCTGG CTGAGCTGTT GAAACTGCAC TATATGCAGG AGTCACCGGG GACCACCCAC 
CCACTGCTCA GGTTTGACTA CGTCCCCAGC TTTTTGTACA AAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078909.1
CDS	504..3311
Translation	MISAPDVVAFTKEDEYEEEPYNEPALPEEYSVPLFPYASQGANPWSKLSGAKFSRDFILISEFSEQVGPQPLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSKASECLKMGNRKAFAGELEKKLKDLDYTRTVLHTETEIQKKANDKGFYSSQAIEKANELANVEKSIIEHQDLLRQIRSYPRQKTKIPDLQPGDTEHTQDQADQVSTTSNPEESANADLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGDLCYLLTSQIDRVLRKQQPITNFLFEDFVEVDDRMEKQENVPSQPSQDRLPPKPVEECPIPKVLISVGSYKSSVESVLIKMEQELGDEEYTGVEATEARSFDPQENLDYLDMDMKGSISSGESIEVLGTEKSASVLSKSDSQASLTVPLSPHVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEEAYADNEGAIHFQASAGSPEPDETQEGNLENIPSQIDSSCCIGKESEGHLVPLPTPAYTLSDEDSVVSIPPQRYIQKDQGLHVDFGVENTDPSPRDNSCEMFPAYELDPSCLLASRDVSKMSLDNYSDTTSYMGSAASTSSDRIPSAPPAGLSSERHKKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDETIVRKLVTALSIFVPNYGCYAKPVKHWISSPLHIMDFQKWKLIGLQRVASPANVGTLHTLSRYSRYTSILDLDSKTLRCPLYRGTLVPRLADHRTQIKRGSTYYLHVQSMLTQLCSKAFLYTFCHHLHLPAHSEETQEAVASRQTSFLKLNLGLVNEDIRVVQYLAELLKLHYMQESPGTTHPLLRFDYVPSFLYKI

Target ORF information:

RefSeq Version	NM_001085440.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) (Smcr8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085440.1

ORF Insert Sequence:

ATGATCAGCG CCCCTGATGT GGTGGCCTTC ACCAAGGAAG ATGAATACGA GGAAGAACCT 
TACAATGAGC CCGCTTTGCC TGAGGAGTAC TCAGTCCCTC TCTTTCCTTA TGCCAGCCAG 
GGGGCAAACC CCTGGTCTAA ACTGTCTGGG GCCAAGTTCT CCAGGGACTT CATCCTCATT 
TCCGAGTTCT CTGAGCAGGT GGGACCCCAG CCCTTGCTTA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT TAATTACTTC TCTTTGCGGA TTATGTCGGT GGATTACCAG 
GCTTCATTCG TAGGCCATCC TCCCGGTTCT GCCTACCCCA AGTTGAACTT TGTGGAAGAC 
TCTAAAGTGG TACTGGGAGA CTCTAAGGAA GGGGCCTTTG CATATGTGCA CCACCTCACC 
TTGTACGACC TGGAGGCCAG GGGCTTTGTG AGGCCCTTTT GTATGGCTTA TATCTCCGCA 
GACCAGCATA AAATCATGCA GCAGTTCCAA GAGCTCTCGG CCGAATTTTC CAAAGCATCT 
GAGTGCTTGA AGATGGGCAA CAGGAAGGCA TTCGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACAAGGAC AGTGCTACAC ACAGAAACCG AGATCCAGAA GAAAGCCAAC 
GACAAGGGTT TTTATTCTTC TCAGGCGATT GAGAAAGCCA ATGAACTGGC CAATGTGGAG 
AAGTCCATCA TCGAACATCA AGATTTGCTG AGGCAGATCC GCTCATACCC TCGTCAGAAG 
ACGAAGATCC CTGACTTGCA GCCTGGTGAT ACAGAGCATA CCCAGGATCA GGCTGACCAG 
GTATCCACTA CCTCTAATCC TGAGGAGTCC GCTAATGCAG ACCTTTATAC CTGCAGACCG 
GCTTACACCC CCAAACTCAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AACTCTGTGA CACTGAGTAT TTCACTCAGA CCCTGGCCCA GCTTAGCCAC 
ATTGAACACA TGTTCAGAGG AGACCTGTGC TACCTCCTGA CCAGTCAGAT TGACAGAGTG 
CTTCGAAAAC AACAGCCCAT AACGAATTTC CTCTTTGAAG ATTTTGTAGA GGTGGATGAC 
AGGATGGAAA AGCAAGAGAA TGTACCCTCT CAGCCCAGTC AGGACAGGCT GCCTCCCAAG 
CCTGTAGAAG AATGCCCCAT TCCTAAAGTG TTAATTAGCG TTGGCTCTTA CAAGTCCAGT 
GTGGAGTCTG TATTGATCAA GATGGAGCAG GAACTTGGCG ATGAAGAGTA CACGGGGGTG 
GAGGCAACAG AGGCACGCAG TTTTGACCCC CAAGAGAACC TGGACTACCT GGATATGGAT 
ATGAAAGGGA GCATCAGTAG TGGGGAAAGC ATTGAGGTGC TGGGCACCGA GAAGTCAGCC 
TCTGTGTTGT CGAAATCTGA CAGCCAGGCC AGCCTCACCG TGCCATTGAG CCCCCATGTA 
GTCCGTAGCA AAGCGGTCAG CCACAGGACA ATCAGCGAGG ACAGCATTGA AGTCTTAAGT 
ACCTGCCCTT CTGAGGCCCT CATTCCTGAT GACTTTAAGG CCAGTTACCC AAGTGCCATT 
AATGAAGAAG AAGCCTATGC GGATAATGAG GGGGCCATCC ATTTCCAGGC AAGTGCCGGC 
TCACCAGAAC CGGATGAGAC TCAGGAGGGC AACTTGGAAA ATATCCCATC CCAAATAGAC 
TCCAGCTGCT GTATTGGAAA GGAGAGTGAA GGTCACTTGG TGCCTCTCCC AACCCCAGCC 
TACACTCTTT CTGATGAGGA CAGTGTGGTG AGCATTCCCC CACAGCGCTA CATACAGAAG 
GACCAGGGGC TCCACGTGGA CTTCGGAGTG GAAAACACTG ACCCTTCTCC CCGAGACAAC 
AGTTGTGAAA TGTTCCCAGC TTATGAGCTG GATCCAAGCT GCCTTCTGGC TAGCCGAGAT 
GTTAGTAAGA TGAGCCTGGA TAACTACTCG GATACCACTA GCTACATGGG CAGTGCGGCC 
TCTACCAGCT CAGACAGAAT CCCCTCAGCA CCTCCTGCTG GCCTATCCTC TGAGAGGCAC 
AAAAAGAGAG CTGGCCAGAA TGCCTTAAAA TTCATCCGCC AGTACCCTTT TGCCCACCCA 
GCCATCTACT CCCTGCTCAG TGGGAGGACA CTTGTGGTCC TGGGCGAAGA TGAAACCATT 
GTCAGGAAGC TGGTGACTGC ACTGTCCATC TTTGTTCCCA ACTATGGCTG CTATGCCAAG 
CCGGTGAAGC ACTGGATTTC TTCCCCTTTG CATATTATGG ATTTCCAGAA GTGGAAGCTT 
ATTGGCCTAC AAAGAGTGGC CTCTCCTGCC AATGTGGGTA CCCTCCATAC CCTGAGCCGT 
TACAGCCGTT ATACAAGCAT CCTGGACCTG GACAGCAAGA CCCTACGCTG TCCCCTATAC 
AGGGGTACAC TGGTACCCCG CCTGGCTGAC CATCGCACTC AGATCAAGCG GGGCAGCACA 
TACTACCTTC ACGTCCAGAG CATGCTCACC CAGCTCTGCT CCAAGGCATT CCTCTACACC 
TTTTGTCACC ACTTGCATCT GCCCGCCCAC AGTGAGGAGA CACAAGAAGC AGTGGCCAGC 
AGACAAACCA GCTTCCTAAA GCTAAACCTG GGGCTCGTGA ACGAGGACAT CAGAGTGGTA 
CAGTACCTGG CTGAGCTGTT GAAACTGCAC TATATGCAGG AGTCACCGGG GACCACCCAC 
CCACTGCTCA GGTTTGACTA CGTCCCCAGC TTTTTGTACA AAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smcr8 ( NM_175491.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780700.1 Mus musculus Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) (Smcr8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu58383
Clone ID Related Accession (Same CDS sequence)	NM_175491.4
Accession Version	NM_175491.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Mus musculus(house mouse)
Product	guanine nucleotide exchange protein SMCR8 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ099053.1 and AK083739.1. On May 2, 2007 this sequence version replaced NM_175491.3. ##Evidence-Data-START## Transcript is intronless :: AK083739.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAGCG CCCCTGATGT GGTGGCCTTC ACCAAGGAAG ATGAATACGA GGAAGAACCT 
TACAATGAGC CCGCTTTGCC TGAGGAGTAC TCAGTCCCTC TCTTTCCTTA TGCCAGCCAG 
GGGGCAAACC CCTGGTCTAA ACTGTCTGGG GCCAAGTTCT CCAGGGACTT CATCCTCATT 
TCCGAGTTCT CTGAGCAGGT GGGACCCCAG CCCTTGCTTA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT TAATTACTTC TCTTTGCGGA TTATGTCGGT GGATTACCAG 
GCTTCATTCG TAGGCCATCC TCCCGGTTCT GCCTACCCCA AGTTGAACTT TGTGGAAGAC 
TCTAAAGTGG TACTGGGAGA CTCTAAGGAA GGGGCCTTTG CATATGTGCA CCACCTCACC 
TTGTACGACC TGGAGGCCAG GGGCTTTGTG AGGCCCTTTT GTATGGCTTA TATCTCCGCA 
GACCAGCATA AAATCATGCA GCAGTTCCAA GAGCTCTCGG CCGAATTTTC CAAAGCATCT 
GAGTGCTTGA AGATGGGCAA CAGGAAGGCA TTCGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACAAGGAC AGTGCTACAC ACAGAAACCG AGATCCAGAA GAAAGCCAAC 
GACAAGGGTT TTTATTCTTC TCAGGCGATT GAGAAAGCCA ATGAACTGGC CAATGTGGAG 
AAGTCCATCA TCGAACATCA AGATTTGCTG AGGCAGATCC GCTCATACCC TCGTCAGAAG 
ACGAAGATCC CTGACTTGCA GCCTGGTGAT ACAGAGCATA CCCAGGATCA GGCTGACCAG 
GTATCCACTA CCTCTAATCC TGAGGAGTCC GCTAATGCAG ACCTTTATAC CTGCAGACCG 
GCTTACACCC CCAAACTCAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AACTCTGTGA CACTGAGTAT TTCACTCAGA CCCTGGCCCA GCTTAGCCAC 
ATTGAACACA TGTTCAGAGG AGACCTGTGC TACCTCCTGA CCAGTCAGAT TGACAGAGTG 
CTTCGAAAAC AACAGCCCAT AACGAATTTC CTCTTTGAAG ATTTTGTAGA GGTGGATGAC 
AGGATGGAAA AGCAAGAGAA TGTACCCTCT CAGCCCAGTC AGGACAGGCT GCCTCCCAAG 
CCTGTAGAAG AATGCCCCAT TCCTAAAGTG TTAATTAGCG TTGGCTCTTA CAAGTCCAGT 
GTGGAGTCTG TATTGATCAA GATGGAGCAG GAACTTGGCG ATGAAGAGTA CACGGGGGTG 
GAGGCAACAG AGGCACGCAG TTTTGACCCC CAAGAGAACC TGGACTACCT GGATATGGAT 
ATGAAAGGGA GCATCAGTAG TGGGGAAAGC ATTGAGGTGC TGGGCACCGA GAAGTCAGCC 
TCTGTGTTGT CGAAATCTGA CAGCCAGGCC AGCCTCACCG TGCCATTGAG CCCCCATGTA 
GTCCGTAGCA AAGCGGTCAG CCACAGGACA ATCAGCGAGG ACAGCATTGA AGTCTTAAGT 
ACCTGCCCTT CTGAGGCCCT CATTCCTGAT GACTTTAAGG CCAGTTACCC AAGTGCCATT 
AATGAAGAAG AAGCCTATGC GGATAATGAG GGGGCCATCC ATTTCCAGGC AAGTGCCGGC 
TCACCAGAAC CGGATGAGAC TCAGGAGGGC AACTTGGAAA ATATCCCATC CCAAATAGAC 
TCCAGCTGCT GTATTGGAAA GGAGAGTGAA GGTCACTTGG TGCCTCTCCC AACCCCAGCC 
TACACTCTTT CTGATGAGGA CAGTGTGGTG AGCATTCCCC CACAGCGCTA CATACAGAAG 
GACCAGGGGC TCCACGTGGA CTTCGGAGTG GAAAACACTG ACCCTTCTCC CCGAGACAAC 
AGTTGTGAAA TGTTCCCAGC TTATGAGCTG GATCCAAGCT GCCTTCTGGC TAGCCGAGAT 
GTTAGTAAGA TGAGCCTGGA TAACTACTCG GATACCACTA GCTACATGGG CAGTGCGGCC 
TCTACCAGCT CAGACAGAAT CCCCTCAGCA CCTCCTGCTG GCCTATCCTC TGAGAGGCAC 
AAAAAGAGAG CTGGCCAGAA TGCCTTAAAA TTCATCCGCC AGTACCCTTT TGCCCACCCA 
GCCATCTACT CCCTGCTCAG TGGGAGGACA CTTGTGGTCC TGGGCGAAGA TGAAACCATT 
GTCAGGAAGC TGGTGACTGC ACTGTCCATC TTTGTTCCCA ACTATGGCTG CTATGCCAAG 
CCGGTGAAGC ACTGGATTTC TTCCCCTTTG CATATTATGG ATTTCCAGAA GTGGAAGCTT 
ATTGGCCTAC AAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780700.1
CDS	504..2861
Translation	MISAPDVVAFTKEDEYEEEPYNEPALPEEYSVPLFPYASQGANPWSKLSGAKFSRDFILISEFSEQVGPQPLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSKASECLKMGNRKAFAGELEKKLKDLDYTRTVLHTETEIQKKANDKGFYSSQAIEKANELANVEKSIIEHQDLLRQIRSYPRQKTKIPDLQPGDTEHTQDQADQVSTTSNPEESANADLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGDLCYLLTSQIDRVLRKQQPITNFLFEDFVEVDDRMEKQENVPSQPSQDRLPPKPVEECPIPKVLISVGSYKSSVESVLIKMEQELGDEEYTGVEATEARSFDPQENLDYLDMDMKGSISSGESIEVLGTEKSASVLSKSDSQASLTVPLSPHVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEEAYADNEGAIHFQASAGSPEPDETQEGNLENIPSQIDSSCCIGKESEGHLVPLPTPAYTLSDEDSVVSIPPQRYIQKDQGLHVDFGVENTDPSPRDNSCEMFPAYELDPSCLLASRDVSKMSLDNYSDTTSYMGSAASTSSDRIPSAPPAGLSSERHKKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDETIVRKLVTALSIFVPNYGCYAKPVKHWISSPLHIMDFQKWKLIGLQR

Target ORF information:

RefSeq Version	NM_175491.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) (Smcr8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175491.4

ORF Insert Sequence:

ATGATCAGCG CCCCTGATGT GGTGGCCTTC ACCAAGGAAG ATGAATACGA GGAAGAACCT 
TACAATGAGC CCGCTTTGCC TGAGGAGTAC TCAGTCCCTC TCTTTCCTTA TGCCAGCCAG 
GGGGCAAACC CCTGGTCTAA ACTGTCTGGG GCCAAGTTCT CCAGGGACTT CATCCTCATT 
TCCGAGTTCT CTGAGCAGGT GGGACCCCAG CCCTTGCTTA CCATCCCCAA TGACACCAAA 
GTTTTTGGCA CTTTTGATCT TAATTACTTC TCTTTGCGGA TTATGTCGGT GGATTACCAG 
GCTTCATTCG TAGGCCATCC TCCCGGTTCT GCCTACCCCA AGTTGAACTT TGTGGAAGAC 
TCTAAAGTGG TACTGGGAGA CTCTAAGGAA GGGGCCTTTG CATATGTGCA CCACCTCACC 
TTGTACGACC TGGAGGCCAG GGGCTTTGTG AGGCCCTTTT GTATGGCTTA TATCTCCGCA 
GACCAGCATA AAATCATGCA GCAGTTCCAA GAGCTCTCGG CCGAATTTTC CAAAGCATCT 
GAGTGCTTGA AGATGGGCAA CAGGAAGGCA TTCGCTGGGG AACTTGAAAA AAAGCTGAAA 
GACTTGGATT ACACAAGGAC AGTGCTACAC ACAGAAACCG AGATCCAGAA GAAAGCCAAC 
GACAAGGGTT TTTATTCTTC TCAGGCGATT GAGAAAGCCA ATGAACTGGC CAATGTGGAG 
AAGTCCATCA TCGAACATCA AGATTTGCTG AGGCAGATCC GCTCATACCC TCGTCAGAAG 
ACGAAGATCC CTGACTTGCA GCCTGGTGAT ACAGAGCATA CCCAGGATCA GGCTGACCAG 
GTATCCACTA CCTCTAATCC TGAGGAGTCC GCTAATGCAG ACCTTTATAC CTGCAGACCG 
GCTTACACCC CCAAACTCAT CAAAGCAAAG TCCACCAAGT GTTTTGACAA GAAGTTGAAG 
ACCTTGGAGG AACTCTGTGA CACTGAGTAT TTCACTCAGA CCCTGGCCCA GCTTAGCCAC 
ATTGAACACA TGTTCAGAGG AGACCTGTGC TACCTCCTGA CCAGTCAGAT TGACAGAGTG 
CTTCGAAAAC AACAGCCCAT AACGAATTTC CTCTTTGAAG ATTTTGTAGA GGTGGATGAC 
AGGATGGAAA AGCAAGAGAA TGTACCCTCT CAGCCCAGTC AGGACAGGCT GCCTCCCAAG 
CCTGTAGAAG AATGCCCCAT TCCTAAAGTG TTAATTAGCG TTGGCTCTTA CAAGTCCAGT 
GTGGAGTCTG TATTGATCAA GATGGAGCAG GAACTTGGCG ATGAAGAGTA CACGGGGGTG 
GAGGCAACAG AGGCACGCAG TTTTGACCCC CAAGAGAACC TGGACTACCT GGATATGGAT 
ATGAAAGGGA GCATCAGTAG TGGGGAAAGC ATTGAGGTGC TGGGCACCGA GAAGTCAGCC 
TCTGTGTTGT CGAAATCTGA CAGCCAGGCC AGCCTCACCG TGCCATTGAG CCCCCATGTA 
GTCCGTAGCA AAGCGGTCAG CCACAGGACA ATCAGCGAGG ACAGCATTGA AGTCTTAAGT 
ACCTGCCCTT CTGAGGCCCT CATTCCTGAT GACTTTAAGG CCAGTTACCC AAGTGCCATT 
AATGAAGAAG AAGCCTATGC GGATAATGAG GGGGCCATCC ATTTCCAGGC AAGTGCCGGC 
TCACCAGAAC CGGATGAGAC TCAGGAGGGC AACTTGGAAA ATATCCCATC CCAAATAGAC 
TCCAGCTGCT GTATTGGAAA GGAGAGTGAA GGTCACTTGG TGCCTCTCCC AACCCCAGCC 
TACACTCTTT CTGATGAGGA CAGTGTGGTG AGCATTCCCC CACAGCGCTA CATACAGAAG 
GACCAGGGGC TCCACGTGGA CTTCGGAGTG GAAAACACTG ACCCTTCTCC CCGAGACAAC 
AGTTGTGAAA TGTTCCCAGC TTATGAGCTG GATCCAAGCT GCCTTCTGGC TAGCCGAGAT 
GTTAGTAAGA TGAGCCTGGA TAACTACTCG GATACCACTA GCTACATGGG CAGTGCGGCC 
TCTACCAGCT CAGACAGAAT CCCCTCAGCA CCTCCTGCTG GCCTATCCTC TGAGAGGCAC 
AAAAAGAGAG CTGGCCAGAA TGCCTTAAAA TTCATCCGCC AGTACCCTTT TGCCCACCCA 
GCCATCTACT CCCTGCTCAG TGGGAGGACA CTTGTGGTCC TGGGCGAAGA TGAAACCATT 
GTCAGGAAGC TGGTGACTGC ACTGTCCATC TTTGTTCCCA ACTATGGCTG CTATGCCAAG 
CCGGTGAAGC ACTGGATTTC TTCCCCTTTG CATATTATGG ATTTCCAGAA GTGGAAGCTT 
ATTGGCCTAC AAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Excessive endosomal TLR signaling causes inflammatory disease in mice with defective SMCR8-WDR41-C9ORF72 complex function.
Proceedings of the National Academy of Sciences of the United States of America115(49)E11523-E11531(2018 12)
McAlpine W,Sun L,Wang KW,Liu A,Jain R,San Miguel M,Wang J,Zhang Z,Hayse B,McAlpine SG,Choi JH,Zhong X,Ludwig S,Russell J,Zhan X,Choi M,Li X,Tang M,Moresco EMY,Beutler B,Turer E

The C9orf72-interacting protein Smcr8 is a negative regulator of autoimmunity and lysosomal exocytosis.
Genes & development32(13-14)929-943(2018 07)
Zhang Y,Burberry A,Wang JY,Sandoe J,Ghosh S,Udeshi ND,Svinkina T,Mordes DA,Mok J,Charlton M,Li QZ,Carr SA,Eggan K

Loss of C9orf72 Enhances Autophagic Activity via Deregulated mTOR and TFEB Signaling.
PLoS genetics12(11)e1006443(2016 Nov)
Ugolino J,Ji YJ,Conchina K,Chu J,Nirujogi RS,Pandey A,Brady NR,Hamacher-Brady A,Wang J

A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy.
Science advances2(9)e1601167(2016 09)
Yang M,Liang C,Swaminathan K,Herrlinger S,Lai F,Shiekhattar R,Chen JF

Penetrance of craniofacial anomalies in mouse models of Smith-Magenis syndrome is modified by genomic sequence surrounding Rai1: not all null alleles are alike.
American journal of human genetics80(3)518-25(2007 Mar)
Yan J,Bi W,Lupski JR

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Smcr8 cDNA ORF clone, Mus musculus(house mouse)

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