smcr8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	smcr8
Entrez Gene ID	100486182
Full Name	Smith-Magenis syndrome chromosome region, candidate 8
General protein information	Preferred Names Smith-Magenis syndrome chromosome region, candidate 8 Names Smith-Magenis syndrome chromosomal region candidate gene 8 protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935844.4	XP_002935890.1	Smith-Magenis syndrome chromosomal region candidate gene 8 protein
XM_002935844.5	XP_002935890.2	guanine nucleotide exchange protein SMCR8

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMCR8	XP_851653.1
Rattus norvegicus (Norway rat)	Smcr8	NP_001178848.1
Homo sapiens (human)	SMCR8	NP_658988.2
Bos taurus (cattle)	SMCR8	XP_003587459.2
Danio rerio (zebrafish)	smcr8a	XP_687034.3
Macaca mulatta (Rhesus monkey)	SMCR8	XP_001095753.1
Xenopus tropicalis (tropical clawed frog)	smcr8	XP_002935890.1
Gallus gallus (chicken)	SMCR8	XP_004945263.1
Pan troglodytes (chimpanzee)	SMCR8	XP_001157693.1
Mus musculus (house mouse)	Smcr8	NP_001078909.1

Gene Ontology

The following smcr8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smcr8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa16490	XM_002935844.4 Latest version!	Xenopus tropicalis Smith-Magenis syndrome chromosome region, candidate 8 (smcr8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa67141	XM_002935844.5 Latest version!	Xenopus tropicalis Smith-Magenis syndrome chromosome region, candidate 8 (smcr8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smcr8 ( XM_002935844.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935890.1 Xenopus tropicalis Smith-Magenis syndrome chromosome region, candidate 8 (smcr8), mRNA.

Price & Availability↑

CloneID	OXa16490
Clone ID Related Accession (Same CDS sequence)	XM_002935844.4
Accession Version	XM_002935844.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Smith-Magenis syndrome chromosomal region candidate gene 8 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935844.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGCT CTCCGGACCT GGTGCCTTTT ACAATGGAGG AGGGGTATGA AGAGCCATTT 
CATGGCGCCT CTGGGCTGCC GGAAGAATAC ACGGTGCCTC TGTTTCCCTT TTCCCAAGCC 
AATAGCAGTC CCTGGTCCAG GCTGTGTGCT GCCAAATTCA GCAGGGACTT TATTCTCATC 
TCAGAGTTCT CTGAGCAAGT GGGGCCCCAG CCTCTTCTTA CCATCCCTGA TGATCAGCGG 
ATTTGTGGCG AGTTTGACCT GAACTACTTC TCCCTGCGCA TCATGTCGGT GGACTACCAG 
GCATCCTTTG TGGGCCACCC CCCGGGTTCA TCTTACCCGA AGCTGAATTT CGTCGAGGAC 
TCTAAGGTCG TCTTGGGAGA CTCCAAAGAA GGAGCTTTTG CCTATGTCCA TCACTTGACC 
CTGTACGACT TGGAGGCGCG AGGCTTCGTC AGGCCCTTTT GCATGGCCTA TATCTCCATG 
GACGAGGAAA AGATAATGAG ACAGTTCTTG GAGCTTTCCA GCGACTTCTC AAAAGCGTCC 
GAGTGCCTGA AAACCGGCAA TAGGAAGGCG TTTGCCAACG AGCTGGAAAA AAAGCTCCGA 
GACCTTGATT ACACCAGGAC CGTTCTACAG AGAGAAATGG AGGAACAGAT GAAGGCCGAC 
GACAAGGGAT TTTACACGAC ACAAACCATC GATAAAGCCA ACGAACTTGC CAGTGTGGAA 
AAATCCATTA TAGAGCATCA GGATTTACTG AAGCAGATCC AGTTATACCC GTACAGGAAA 
ATAAAAGAGC CGGATTTTCA GCCGTATGAG CCAGAAAGTT TACAAGACTC CGATGTTCCG 
AACGAATCTG AGCAACCATT GCCCCCTTCT AACCCAGAAG AAAGCCTGGT GGAAGAGGAG 
AGCAGTCTTT ACCATCCCAG GTCATCCTAC ACTCCTAAAT TCATAAAGGC AAAATCCTCG 
AAATGTTTTG ATAAGAAATT AAAAACATTG GAGGAACTGG TTGATATTTA TTTCTTTACT 
CAAACAATAG ACCTACTGAC TAGTATAGAA AAACGATACC GGGGTGACTT GAGTTATCTC 
CTAATGAAGC AACTTGAAAA GGGACTTTTA GAGCAACAGA CGGTTTCCAA TTTCTTGTTT 
GAAGATATTG CGACTTGGGA TGCAAAGCTG TCCAGCATGC ATTGTTCCAA TACTTTGGCG 
CTTGTTCAGG CTTTGCAGAA TCCTACATTT TTGGAAGAAC CGGGAAACCT TAAAAATTCA 
GCAAGTCTGG AGTCGTACAA ATCAAGCGTG GAATCTGTCC CTATTAAAAT TGAACAGGAA 
GTATTTGAAG AAGGAAATCA TCAAGAGACG GCTGAGCGCG GTGTTTCCGA CATCCCAGAT 
AACTGTGAAT TTAACGACGT GGATTTTAAA GGGAGTGTTA GTAGCAGCGA CAGCATTGAA 
GTCTTGAGAA CAGAGAAGTC AGTCGCTTTG CTCACTCATT CTGAGAGCCA GCCATGTTTG 
CGACTTAGCC CCCAACCGCC CAGGAGGAAG GTAGGTAGCA GAAGAACCAT TAGTGAGGAC 
AGTATTGAAG TTCTAAGCAC GTGTCCCTCT GAAAGTGTAA TTCCTGAGGA TTTTAGACCA 
GCATATCAAA TCGCCATTCA TGAAGAGGAT CCTGATGGAG TAGATGTGGA TGGAGAACAG 
CCTTGCAATT CCACCAATAA CGAGGAGAAT GAGCCTTCTC CAAACGATTT ATCAGGCGAC 
CCAGCTTGTT GCATGGGGGC AGAAACACTT TTAGCGCAGA CAGGAAATGT GACCCCTGTA 
CCTAATGATG GGGACATTAA TAAAGTGGTT AGTGTCCCGC CACAGTCGTA CAGAAACATG 
GATCATGCCT TTCAAGTCAA GTTCTCAGTA GACAGGGCTG AGAACCATTC TAATAAAGTG 
AGTCCCCATG GCCTCTCTAG TCCTGAAGCA GAGTCAACAT ACTTAATGAG TAGCGGAGAG 
TCTGTAAGAC TAAGCATTGA GGAGTATTCG GACTCTACAA GTTATAGAAG CAGCACGTCG 
ACTTGTTCTG ATAGGACACC CTCCCCGGCC CCATGTGTCA GTGGCCTCTC TGAACGACAT 
AGAAGGAAAG CCGGCCAGAA TGCACTAATG TTCATTAGAC AGTACCCCTT CTCCCACCCT 
GCCATATATT CTCTTCTCTG TGGAAGGACC CTCATAGTGC TGGGTGAGGA GGAAGCCTTT 
GTTAGAAGGC TTGTGACGGC ACTATCAATT TTTGTGCCCA ATCATGGACA TGTTGCCAAA 
CCCGTTAACA CATGGATGAC TTCCCCACTG CACATAATGG ATTTTCAGAA GTGGAAACTA 
ATTGGACTTC AAAGAGGGGG TTCTCCGTCC AGTACCAACA TCCTGCACTC CTTGAACCGC 
TACAGCCGCT ACGCCAGCGT GTTGGACGCA GACCATAAAA CACTACGCTG CCCCGTATAT 
AAAGGAGCCT TCCTGCAGAG ACTGGCTGAT CACCGCACAC AGATCAGGCG GGGCAGTACC 
TACTACATGC ATGTGCAAAG TATGTTGACT CAGCTCAGCG CTAAGGCCTT CCTCTTCACT 
TTTTGTCACC ACGCGCACCT CCCCATCCGG GAAAAAGAAA GCGAGGAGTC CATCGCCCAG 
CGCAGAGCCG CCTTCCTTCG GCAGCTGCTC GGCCTCTCCG AGGAGGACAG CAAGATAGTG 
GAATATCTCT CAGAGCTTCT CAAGATGCAT TATCTGAGGG AATCAGGGAC CCAGCTCCTC 
CCAGTGCTCA GGTTTGACTA TATCCCCAAC TTTTTATACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935890.1
CDS	102..2909
Translation	MIGSPDLVPFTMEEGYEEPFHGASGLPEEYTVPLFPFSQANSSPWSRLCAAKFSRDFILISEFSEQVGPQPLLTIPDDQRICGEFDLNYFSLRIMSVDYQASFVGHPPGSSYPKLNFVEDSKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISMDEEKIMRQFLELSSDFSKASECLKTGNRKAFANELEKKLRDLDYTRTVLQREMEEQMKADDKGFYTTQTIDKANELASVEKSIIEHQDLLKQIQLYPYRKIKEPDFQPYEPESLQDSDVPNESEQPLPPSNPEESLVEEESSLYHPRSSYTPKFIKAKSSKCFDKKLKTLEELVDIYFFTQTIDLLTSIEKRYRGDLSYLLMKQLEKGLLEQQTVSNFLFEDIATWDAKLSSMHCSNTLALVQALQNPTFLEEPGNLKNSASLESYKSSVESVPIKIEQEVFEEGNHQETAERGVSDIPDNCEFNDVDFKGSVSSSDSIEVLRTEKSVALLTHSESQPCLRLSPQPPRRKVGSRRTISEDSIEVLSTCPSESVIPEDFRPAYQIAIHEEDPDGVDVDGEQPCNSTNNEENEPSPNDLSGDPACCMGAETLLAQTGNVTPVPNDGDINKVVSVPPQSYRNMDHAFQVKFSVDRAENHSNKVSPHGLSSPEAESTYLMSSGESVRLSIEEYSDSTSYRSSTSTCSDRTPSPAPCVSGLSERHRRKAGQNALMFIRQYPFSHPAIYSLLCGRTLIVLGEEEAFVRRLVTALSIFVPNHGHVAKPVNTWMTSPLHIMDFQKWKLIGLQRGGSPSSTNILHSLNRYSRYASVLDADHKTLRCPVYKGAFLQRLADHRTQIRRGSTYYMHVQSMLTQLSAKAFLFTFCHHAHLPIREKESEESIAQRRAAFLRQLLGLSEEDSKIVEYLSELLKMHYLRESGTQLLPVLRFDYIPNFLYKI

Target ORF information:

RefSeq Version	XM_002935844.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Smith-Magenis syndrome chromosome region, candidate 8 (smcr8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935844.4

ORF Insert Sequence:

ATGATAGGCT CTCCGGACCT GGTGCCTTTT ACAATGGAGG AGGGGTATGA AGAGCCATTT 
CATGGCGCCT CTGGGCTGCC GGAAGAATAC ACGGTGCCTC TGTTTCCCTT TTCCCAAGCC 
AATAGCAGTC CCTGGTCCAG GCTGTGTGCT GCCAAATTCA GCAGGGACTT TATTCTCATC 
TCAGAGTTCT CTGAGCAAGT GGGGCCCCAG CCTCTTCTTA CCATCCCTGA TGATCAGCGG 
ATTTGTGGCG AGTTTGACCT GAACTACTTC TCCCTGCGCA TCATGTCGGT GGACTACCAG 
GCATCCTTTG TGGGCCACCC CCCGGGTTCA TCTTACCCGA AGCTGAATTT CGTCGAGGAC 
TCTAAGGTCG TCTTGGGAGA CTCCAAAGAA GGAGCTTTTG CCTATGTCCA TCACTTGACC 
CTGTACGACT TGGAGGCGCG AGGCTTCGTC AGGCCCTTTT GCATGGCCTA TATCTCCATG 
GACGAGGAAA AGATAATGAG ACAGTTCTTG GAGCTTTCCA GCGACTTCTC AAAAGCGTCC 
GAGTGCCTGA AAACCGGCAA TAGGAAGGCG TTTGCCAACG AGCTGGAAAA AAAGCTCCGA 
GACCTTGATT ACACCAGGAC CGTTCTACAG AGAGAAATGG AGGAACAGAT GAAGGCCGAC 
GACAAGGGAT TTTACACGAC ACAAACCATC GATAAAGCCA ACGAACTTGC CAGTGTGGAA 
AAATCCATTA TAGAGCATCA GGATTTACTG AAGCAGATCC AGTTATACCC GTACAGGAAA 
ATAAAAGAGC CGGATTTTCA GCCGTATGAG CCAGAAAGTT TACAAGACTC CGATGTTCCG 
AACGAATCTG AGCAACCATT GCCCCCTTCT AACCCAGAAG AAAGCCTGGT GGAAGAGGAG 
AGCAGTCTTT ACCATCCCAG GTCATCCTAC ACTCCTAAAT TCATAAAGGC AAAATCCTCG 
AAATGTTTTG ATAAGAAATT AAAAACATTG GAGGAACTGG TTGATATTTA TTTCTTTACT 
CAAACAATAG ACCTACTGAC TAGTATAGAA AAACGATACC GGGGTGACTT GAGTTATCTC 
CTAATGAAGC AACTTGAAAA GGGACTTTTA GAGCAACAGA CGGTTTCCAA TTTCTTGTTT 
GAAGATATTG CGACTTGGGA TGCAAAGCTG TCCAGCATGC ATTGTTCCAA TACTTTGGCG 
CTTGTTCAGG CTTTGCAGAA TCCTACATTT TTGGAAGAAC CGGGAAACCT TAAAAATTCA 
GCAAGTCTGG AGTCGTACAA ATCAAGCGTG GAATCTGTCC CTATTAAAAT TGAACAGGAA 
GTATTTGAAG AAGGAAATCA TCAAGAGACG GCTGAGCGCG GTGTTTCCGA CATCCCAGAT 
AACTGTGAAT TTAACGACGT GGATTTTAAA GGGAGTGTTA GTAGCAGCGA CAGCATTGAA 
GTCTTGAGAA CAGAGAAGTC AGTCGCTTTG CTCACTCATT CTGAGAGCCA GCCATGTTTG 
CGACTTAGCC CCCAACCGCC CAGGAGGAAG GTAGGTAGCA GAAGAACCAT TAGTGAGGAC 
AGTATTGAAG TTCTAAGCAC GTGTCCCTCT GAAAGTGTAA TTCCTGAGGA TTTTAGACCA 
GCATATCAAA TCGCCATTCA TGAAGAGGAT CCTGATGGAG TAGATGTGGA TGGAGAACAG 
CCTTGCAATT CCACCAATAA CGAGGAGAAT GAGCCTTCTC CAAACGATTT ATCAGGCGAC 
CCAGCTTGTT GCATGGGGGC AGAAACACTT TTAGCGCAGA CAGGAAATGT GACCCCTGTA 
CCTAATGATG GGGACATTAA TAAAGTGGTT AGTGTCCCGC CACAGTCGTA CAGAAACATG 
GATCATGCCT TTCAAGTCAA GTTCTCAGTA GACAGGGCTG AGAACCATTC TAATAAAGTG 
AGTCCCCATG GCCTCTCTAG TCCTGAAGCA GAGTCAACAT ACTTAATGAG TAGCGGAGAG 
TCTGTAAGAC TAAGCATTGA GGAGTATTCG GACTCTACAA GTTATAGAAG CAGCACGTCG 
ACTTGTTCTG ATAGGACACC CTCCCCGGCC CCATGTGTCA GTGGCCTCTC TGAACGACAT 
AGAAGGAAAG CCGGCCAGAA TGCACTAATG TTCATTAGAC AGTACCCCTT CTCCCACCCT 
GCCATATATT CTCTTCTCTG TGGAAGGACC CTCATAGTGC TGGGTGAGGA GGAAGCCTTT 
GTTAGAAGGC TTGTGACGGC ACTATCAATT TTTGTGCCCA ATCATGGACA TGTTGCCAAA 
CCCGTTAACA CATGGATGAC TTCCCCACTG CACATAATGG ATTTTCAGAA GTGGAAACTA 
ATTGGACTTC AAAGAGGGGG TTCTCCGTCC AGTACCAACA TCCTGCACTC CTTGAACCGC 
TACAGCCGCT ACGCCAGCGT GTTGGACGCA GACCATAAAA CACTACGCTG CCCCGTATAT 
AAAGGAGCCT TCCTGCAGAG ACTGGCTGAT CACCGCACAC AGATCAGGCG GGGCAGTACC 
TACTACATGC ATGTGCAAAG TATGTTGACT CAGCTCAGCG CTAAGGCCTT CCTCTTCACT 
TTTTGTCACC ACGCGCACCT CCCCATCCGG GAAAAAGAAA GCGAGGAGTC CATCGCCCAG 
CGCAGAGCCG CCTTCCTTCG GCAGCTGCTC GGCCTCTCCG AGGAGGACAG CAAGATAGTG 
GAATATCTCT CAGAGCTTCT CAAGATGCAT TATCTGAGGG AATCAGGGAC CCAGCTCCTC 
CCAGTGCTCA GGTTTGACTA TATCCCCAAC TTTTTATACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smcr8 ( XM_002935844.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935890.2 Xenopus tropicalis Smith-Magenis syndrome chromosome region, candidate 8 (smcr8), mRNA.

Price & Availability↑

CloneID	OXa67141
Clone ID Related Accession (Same CDS sequence)	XM_002935844.5
Accession Version	XM_002935844.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange protein SMCR8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935844.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGCT CTCCGGACCT GGTGCCTTTT ACAATGGAGG AGGGGTATGA AGAGCCATTT 
CATGGCGCCT CTGGGCTGCC GGAAGAATAC ACGGTGCCTC TGTTTCCCTT TTCCCAAGCC 
AATAGCAGTC CCTGGTCCAG GCTGTGTGCT GCCAAATTCA GCAGGGACTT TATTCTCATC 
TCAGAGTTCT CTGAGCAAGT GGGGCCCCAG CCTCTTCTTA CCATCCCTGA TGATCAGCGG 
ATTTGTGGCG AGTTTGACCT GAACTACTTC TCCCTGCGCA TCATGTCGGT GGACTACCAG 
GCATCCTTTG TGGGCCACCC CCCGGGTTCA TCTTACCCGA AGCTGAATTT CGTCGAGGAC 
TCTAAGGTCG TCTTGGGAGA CTCCAAAGAA GGAGCTTTTG CCTATGTCCA TCACTTGACC 
CTGTACGACT TGGAGGCGCG AGGCTTCGTC AGGCCCTTTT GCATGGCCTA TATCTCCATG 
GACGAGGAAA AGATAATGAG ACAGTTCTTG GAGCTTTCCA GCGACTTCTC AAAAGCGTCC 
AAGTGCCTGA AAACCGGCAA TAGGAAGGCG TTTGCCAACG AGCTGGAAAA AAAGCTCCGA 
GACCTTGATT ACACCAGGAC CGTTCTACAG AGAGAAATGG AAGAACAGAT GAAGGCCGAC 
GACAAGGGAT TTTACACGAC ACAAACCATC GATAAAGCCA ACGAACTTGC CAGTGTGGAA 
AAATCCATTA TAGAGCATCA GGATTTACTG AAGCAGATCC AGTTATACCC GTACAGGAAA 
ATAAAAGAGC CGGATTTTCA GCCGTATGAG CCAGAAAGTT TACAAGACTC CGATGTTCCG 
AATGAATCTG AGCAACCATT GCCCCCTTCT AACCCAGAAG AAAGCCTGGT GGAAGAGGAG 
AGCAGTCTTT ACCATCCCAG GTCATCCTAC ACTCCTAAAT TCATAAAGGC AAAATCCTCA 
AAATGTTTTG ATAAGAAATT AAAAACATTG GAGGAACTGG TTGATATTTA TTTCTTTACT 
CAAACAATAG ACCTACTGAC TAGTATAGAA AAACGATACC GGGGTGACTT GAGTTATCTC 
CTAATGAAGC AACTTGAAAA GGGACTTTTA GAGCAACAGA CGGTTTCCAA TTTCTTGTTT 
GAAGATATTG CGACTTGGGA TGCAAAGCTG TCCAGCATGC ATTGTTCCAA TACTTTGGCG 
CTTGTTCAGG CTTTGCAGAA TCCTACATTT TTGGAAGAAC CGGGAAACCT TAAAAATTCA 
GCAAGTCTGG AGTCGTACAA ATCAAGCGTG GAATCTGTCC CTATTAAAAT TGAACAGGAA 
GTATTTGAAG AAGGAAATCA TCAAGAGACG GCTGAGCGCG GTGTTTCCGA CATCCCAGAT 
AACTGTGAAT TTAACGATGT GGATTTTAAA GGGAGTGTTA GTAGCAGCGA CAGCATTGAA 
GTCTTGAGAA CAGAGAAGTC AGTTGCGTTG CTCACTCATT CTGAGAGCCA GCCATGTTTG 
CGACTTAGCC CCCAACCGCC CAGGAGGAAG GTAGGTAGCA GAAGAACCAT TAGTGAGGAC 
AGTATTGAAG TTCTAAGCAC GTGTCCCTCT GAAAGTGTAA TTCCTGAGGA TTTTAGACCA 
GCATATCAAA TCGCCATTCA TGAAGAGGAT CCTGATGGAG TAGATGTGGA TGGAGAACAG 
CCTTGCAATT CCACCAATAA TGAGGAGAAT GAGCCTTCTC CAAATGATTT ATCAGGCGAC 
CCAGCTTGTT GCATGGGGGC AGAAACACTT TTAGCGCAGA CAGGAAATGT GACCCCTGTA 
CCTAATGATG GGGACATTAA TAAAGTGGTT AGTGTCCCGC CACAGTCGTA CAGAAACATG 
GATCATGCCT TTCAAGTCAA GTTCTCAGTA GACAGGGCTG AGAACCATTC TAATAAAGTG 
AGTCCCCATG GCCTCTCTAG TCCTGAAGCA GAGTCAACAT ACTTAATGAG TAGCGGAGAG 
TCTGTAAGAC TAAGCATTGA GGAGTATTCG GACTCTACAA GTTATAGAAG CAGCACGTCG 
ACTTGTTCTG ATAGGACACC CTCCCCGGCC CCATGTGTCA GTGGCCTCTC TGAACGACAT 
AGAAGGAAAG CCGGCCAGAA TGCACTAATG TTCATTAGAC AGTACCCCTT CTCCCACCCT 
GCCATATATT CTCTTCTCTG TGGAAGGACC CTCATAGTGC TGGGTGAGGA GGAAGCCTTT 
GTTAGAAGGC TTGTGACGGC ACTATCAATT TTTGTGCCCA ATCATGGACA TGTTGCCAAA 
CCCGTTAACA CATGGATGAC TTCCCCACTG CACATAATGG ATTTTCAGAA GTGGAAACTA 
ATTGGACTTC AAAGAGGGGG TTCTCCGTCC AGTACCAACA TCCTGCACTC CTTGAACCGC 
TACAGCCGCT ACGCCAGCGT GTTGGACGCA GACCATAAAA CACTACGCTG CCCCGTATAT 
AAAGGAGCCT TCCTGCAGAG ACTGGCTGAT CACCGCACAC AGATCAGGCG GGGCAGTACC 
TACTACATGC ATGTGCAAAG TATGTTGACT CAGCTCAGCG CTAAGGCCTT CCTCTTCACT 
TTTTGTCACC ACGCGCACCT CCCCATCCGG GAAAAAGAAA GCGAGGAGTC CATCGCCCAG 
CGCAGAGCCG CCTTCCTTCG GCAGCTGCTC GGCCTCTCCG AGGAGGACAG CAAGATAGTG 
GAATATCTCT CAGAGCTTCT CAAGATGCAT TATCTGAGGG AATCAGGGAC CCAGCTCCTC 
CCAGTGCTCA GGTTTGACTA TATCCCCAAC TTTTTATACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935890.2
CDS	119..2926
Translation	MIGSPDLVPFTMEEGYEEPFHGASGLPEEYTVPLFPFSQANSSPWSRLCAAKFSRDFILISEFSEQVGPQPLLTIPDDQRICGEFDLNYFSLRIMSVDYQASFVGHPPGSSYPKLNFVEDSKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISMDEEKIMRQFLELSSDFSKASKCLKTGNRKAFANELEKKLRDLDYTRTVLQREMEEQMKADDKGFYTTQTIDKANELASVEKSIIEHQDLLKQIQLYPYRKIKEPDFQPYEPESLQDSDVPNESEQPLPPSNPEESLVEEESSLYHPRSSYTPKFIKAKSSKCFDKKLKTLEELVDIYFFTQTIDLLTSIEKRYRGDLSYLLMKQLEKGLLEQQTVSNFLFEDIATWDAKLSSMHCSNTLALVQALQNPTFLEEPGNLKNSASLESYKSSVESVPIKIEQEVFEEGNHQETAERGVSDIPDNCEFNDVDFKGSVSSSDSIEVLRTEKSVALLTHSESQPCLRLSPQPPRRKVGSRRTISEDSIEVLSTCPSESVIPEDFRPAYQIAIHEEDPDGVDVDGEQPCNSTNNEENEPSPNDLSGDPACCMGAETLLAQTGNVTPVPNDGDINKVVSVPPQSYRNMDHAFQVKFSVDRAENHSNKVSPHGLSSPEAESTYLMSSGESVRLSIEEYSDSTSYRSSTSTCSDRTPSPAPCVSGLSERHRRKAGQNALMFIRQYPFSHPAIYSLLCGRTLIVLGEEEAFVRRLVTALSIFVPNHGHVAKPVNTWMTSPLHIMDFQKWKLIGLQRGGSPSSTNILHSLNRYSRYASVLDADHKTLRCPVYKGAFLQRLADHRTQIRRGSTYYMHVQSMLTQLSAKAFLFTFCHHAHLPIREKESEESIAQRRAAFLRQLLGLSEEDSKIVEYLSELLKMHYLRESGTQLLPVLRFDYIPNFLYKI

Target ORF information:

RefSeq Version	XM_002935844.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Smith-Magenis syndrome chromosome region, candidate 8 (smcr8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935844.5

ORF Insert Sequence:

ATGATAGGCT CTCCGGACCT GGTGCCTTTT ACAATGGAGG AGGGGTATGA AGAGCCATTT 
CATGGCGCCT CTGGGCTGCC GGAAGAATAC ACGGTGCCTC TGTTTCCCTT TTCCCAAGCC 
AATAGCAGTC CCTGGTCCAG GCTGTGTGCT GCCAAATTCA GCAGGGACTT TATTCTCATC 
TCAGAGTTCT CTGAGCAAGT GGGGCCCCAG CCTCTTCTTA CCATCCCTGA TGATCAGCGG 
ATTTGTGGCG AGTTTGACCT GAACTACTTC TCCCTGCGCA TCATGTCGGT GGACTACCAG 
GCATCCTTTG TGGGCCACCC CCCGGGTTCA TCTTACCCGA AGCTGAATTT CGTCGAGGAC 
TCTAAGGTCG TCTTGGGAGA CTCCAAAGAA GGAGCTTTTG CCTATGTCCA TCACTTGACC 
CTGTACGACT TGGAGGCGCG AGGCTTCGTC AGGCCCTTTT GCATGGCCTA TATCTCCATG 
GACGAGGAAA AGATAATGAG ACAGTTCTTG GAGCTTTCCA GCGACTTCTC AAAAGCGTCC 
AAGTGCCTGA AAACCGGCAA TAGGAAGGCG TTTGCCAACG AGCTGGAAAA AAAGCTCCGA 
GACCTTGATT ACACCAGGAC CGTTCTACAG AGAGAAATGG AAGAACAGAT GAAGGCCGAC 
GACAAGGGAT TTTACACGAC ACAAACCATC GATAAAGCCA ACGAACTTGC CAGTGTGGAA 
AAATCCATTA TAGAGCATCA GGATTTACTG AAGCAGATCC AGTTATACCC GTACAGGAAA 
ATAAAAGAGC CGGATTTTCA GCCGTATGAG CCAGAAAGTT TACAAGACTC CGATGTTCCG 
AATGAATCTG AGCAACCATT GCCCCCTTCT AACCCAGAAG AAAGCCTGGT GGAAGAGGAG 
AGCAGTCTTT ACCATCCCAG GTCATCCTAC ACTCCTAAAT TCATAAAGGC AAAATCCTCA 
AAATGTTTTG ATAAGAAATT AAAAACATTG GAGGAACTGG TTGATATTTA TTTCTTTACT 
CAAACAATAG ACCTACTGAC TAGTATAGAA AAACGATACC GGGGTGACTT GAGTTATCTC 
CTAATGAAGC AACTTGAAAA GGGACTTTTA GAGCAACAGA CGGTTTCCAA TTTCTTGTTT 
GAAGATATTG CGACTTGGGA TGCAAAGCTG TCCAGCATGC ATTGTTCCAA TACTTTGGCG 
CTTGTTCAGG CTTTGCAGAA TCCTACATTT TTGGAAGAAC CGGGAAACCT TAAAAATTCA 
GCAAGTCTGG AGTCGTACAA ATCAAGCGTG GAATCTGTCC CTATTAAAAT TGAACAGGAA 
GTATTTGAAG AAGGAAATCA TCAAGAGACG GCTGAGCGCG GTGTTTCCGA CATCCCAGAT 
AACTGTGAAT TTAACGATGT GGATTTTAAA GGGAGTGTTA GTAGCAGCGA CAGCATTGAA 
GTCTTGAGAA CAGAGAAGTC AGTTGCGTTG CTCACTCATT CTGAGAGCCA GCCATGTTTG 
CGACTTAGCC CCCAACCGCC CAGGAGGAAG GTAGGTAGCA GAAGAACCAT TAGTGAGGAC 
AGTATTGAAG TTCTAAGCAC GTGTCCCTCT GAAAGTGTAA TTCCTGAGGA TTTTAGACCA 
GCATATCAAA TCGCCATTCA TGAAGAGGAT CCTGATGGAG TAGATGTGGA TGGAGAACAG 
CCTTGCAATT CCACCAATAA TGAGGAGAAT GAGCCTTCTC CAAATGATTT ATCAGGCGAC 
CCAGCTTGTT GCATGGGGGC AGAAACACTT TTAGCGCAGA CAGGAAATGT GACCCCTGTA 
CCTAATGATG GGGACATTAA TAAAGTGGTT AGTGTCCCGC CACAGTCGTA CAGAAACATG 
GATCATGCCT TTCAAGTCAA GTTCTCAGTA GACAGGGCTG AGAACCATTC TAATAAAGTG 
AGTCCCCATG GCCTCTCTAG TCCTGAAGCA GAGTCAACAT ACTTAATGAG TAGCGGAGAG 
TCTGTAAGAC TAAGCATTGA GGAGTATTCG GACTCTACAA GTTATAGAAG CAGCACGTCG 
ACTTGTTCTG ATAGGACACC CTCCCCGGCC CCATGTGTCA GTGGCCTCTC TGAACGACAT 
AGAAGGAAAG CCGGCCAGAA TGCACTAATG TTCATTAGAC AGTACCCCTT CTCCCACCCT 
GCCATATATT CTCTTCTCTG TGGAAGGACC CTCATAGTGC TGGGTGAGGA GGAAGCCTTT 
GTTAGAAGGC TTGTGACGGC ACTATCAATT TTTGTGCCCA ATCATGGACA TGTTGCCAAA 
CCCGTTAACA CATGGATGAC TTCCCCACTG CACATAATGG ATTTTCAGAA GTGGAAACTA 
ATTGGACTTC AAAGAGGGGG TTCTCCGTCC AGTACCAACA TCCTGCACTC CTTGAACCGC 
TACAGCCGCT ACGCCAGCGT GTTGGACGCA GACCATAAAA CACTACGCTG CCCCGTATAT 
AAAGGAGCCT TCCTGCAGAG ACTGGCTGAT CACCGCACAC AGATCAGGCG GGGCAGTACC 
TACTACATGC ATGTGCAAAG TATGTTGACT CAGCTCAGCG CTAAGGCCTT CCTCTTCACT 
TTTTGTCACC ACGCGCACCT CCCCATCCGG GAAAAAGAAA GCGAGGAGTC CATCGCCCAG 
CGCAGAGCCG CCTTCCTTCG GCAGCTGCTC GGCCTCTCCG AGGAGGACAG CAAGATAGTG 
GAATATCTCT CAGAGCTTCT CAAGATGCAT TATCTGAGGG AATCAGGGAC CCAGCTCCTC 
CCAGTGCTCA GGTTTGACTA TATCCCCAAC TTTTTATACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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smcr8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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