ORF » Species Summary » Danio rerio » espl1 cDNA ORF clone

espl1 cDNA ORF clone, Danio rerio(zebrafish)

The following espl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the espl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa12112 XM_021477220.1
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa12112 XM_001337833.5
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa12112 XM_017356662.1
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa12113 XM_005166094.4
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa12113 XM_021477221.1
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa144755 XM_021471828.1
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa144756 XM_021471829.1
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa146569 XM_021474123.1
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa146570 XM_021474124.1
Latest version!		 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
View Old Accession Versions >>
ODa12113 XM_005166094.3 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa12112 XM_001337833.4 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    espl1 ( XM_021477220.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332895.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa12112
    Clone ID Related Accession (Same CDS sequence) XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
    Accession Version XM_021477220.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332895.1
    CDS210..6758
    Translation

    Target ORF information:

    RefSeq Version XM_021477220.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021477220.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_001337833.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001337869.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa12112
    Clone ID Related Accession (Same CDS sequence) XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
    Accession Version XM_001337833.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product separin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001337833.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001337869.1
    CDS187..6735
    Translation

    Target ORF information:

    RefSeq Version XM_001337833.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001337833.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_001337833.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001337869.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa12112
    Clone ID Related Accession (Same CDS sequence) XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
    Accession Version XM_001337833.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001337833.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001337869.1
    CDS210..6758
    Translation

    Target ORF information:

    RefSeq Version XM_001337833.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001337833.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_017356662.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212151.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa12112
    Clone ID Related Accession (Same CDS sequence) XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
    Accession Version XM_017356662.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product separin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212151.1
    CDS138..6686
    Translation

    Target ORF information:

    RefSeq Version XM_017356662.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017356662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
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    3181
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    3481
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    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
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    5641
    5701
    5761
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    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_005166094.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166151.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa12113
    Clone ID Related Accession (Same CDS sequence) XM_005166094.3 , XM_005166094.4 , XM_021477221.1
    Accession Version XM_005166094.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product separin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1981
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    5941
    6001
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    6301
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    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166151.1
    CDS187..6732
    Translation

    Target ORF information:

    RefSeq Version XM_005166094.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005166094.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_005166094.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166151.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa12113
    Clone ID Related Accession (Same CDS sequence) XM_005166094.3 , XM_005166094.4 , XM_021477221.1
    Accession Version XM_005166094.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product separin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166094.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166151.1
    CDS210..6755
    Translation

    Target ORF information:

    RefSeq Version XM_005166094.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005166094.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_021477221.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332896.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa12113
    Clone ID Related Accession (Same CDS sequence) XM_005166094.3 , XM_005166094.4 , XM_021477221.1
    Accession Version XM_021477221.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product separin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332896.1
    CDS210..6755
    Translation

    Target ORF information:

    RefSeq Version XM_021477221.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021477221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_021471828.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327503.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa144755
    Clone ID Related Accession (Same CDS sequence) XM_021471828.1
    Accession Version XM_021471828.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product separin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
    GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT 
    GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA 
    CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT 
    AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC 
    TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA 
    CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC 
    AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC 
    GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT 
    GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG 
    GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA 
    CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG 
    CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA 
    GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC 
    ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG 
    GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG 
    GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA 
    TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT 
    TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA 
    GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG 
    TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT 
    GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG 
    GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG 
    GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT 
    CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC 
    GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG 
    GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC 
    ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC 
    CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG 
    GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG 
    CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC 
    GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA 
    GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT 
    CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC 
    GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT 
    TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC 
    ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA 
    GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT 
    AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT 
    GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC 
    ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA 
    CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT 
    TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT 
    CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG 
    ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA 
    TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA 
    GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA 
    ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG 
    GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG 
    AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA 
    GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA 
    GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT 
    GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG 
    GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG 
    CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC 
    AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC 
    GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA 
    CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA 
    CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT 
    GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG 
    ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT 
    AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG 
    TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT 
    CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT 
    AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA 
    GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC 
    AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG 
    AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA 
    CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC 
    CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT 
    AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT 
    GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC 
    ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA 
    GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT 
    GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA 
    CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT 
    AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC 
    ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA 
    GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA 
    GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG CAGACTTGTC AGTAGAGACG 
    GTCCAGTCAT ACCTGCGCTC CTCATGGCTC CTCCTGCACC ACTTCCCGTT TCCTTCTCTT 
    TATCCTCACA TCTGCTCATT ACTCGCTCAG TCATTCGGTC AAACGGACCC CATCATCACA 
    GCAATGCTGC ATGCACAGGC ACTGGGAGTG TCCACTCGCC ACCATATGAC CCGTCACGTG 
    GTTAACCAGT TCAGGAAGTT GAAGAAATCC TGTAACGATG TAGCAGAAGG TCTTGGAGCT 
    CTGAGTCTGG AGGAAACATC TGGGGCAACG CAGTCAAAGA CACTCTCTGC GTTGGAGCAG 
    ATCTTTTCAT TCACAGCAGC AAACCCAATG CAGTTTCCAC AGACACACTG TCAGCAGTTC 
    ACACAGCAGC TGGAAGATCT ACCTGCAGGA GTGACCGTAT GCTTGCTGTC ACTGACGGGG 
    GTGCATCCTG ATGAAATTGG CAACACTATT CTTCTGACCC GACTGGAGCG CGGCTCTACC 
    CCAATCACAG TTCGAATCCC CACTGCAGAC AGAGAGCAAT CTGTTGCTGG CCTGTTGGAA 
    GAAATGGATG GAGTCTTGAA AGGACAAAAG GAAGTGAGCA CAGTGGCTGA GAAATCACAG 
    TGGTGGGACG GAAGGAAGGC TTTAGATGCC CGCGTGGAGA AAATGCTGGA GGAGATGGAA 
    GAGGCTTTAG GAGTGTGGCG AACTCTTCTT CTGCCTTTGA CCTCTGATCC TAAGCTTGAT 
    GTTCAGGTAA AGAGCCTCCA AAAGGCTCTT AAATCAACAA AGATCACACG GGACACTCTC 
    AAGGTGATTC TTTCAGCGTC TCCACTGCTG TCCCTGCCAG ACCTGCAGTG TCTTCTGGAA 
    GGAATGAATG TGCAGGACAA GGACCTGCTG AAGCTCCTGC AGAGGGCCGT GGCTGAACTG 
    AGAGGGAGGG AGGAGCAACA GGGACACACT GTTCTGATTT TAGACAAGTA CTTGCAGAGG 
    TTGCCGTGGG AGAACATTGC TTGTCTGAAG TCTCAGTCGG TCACTCGAAT GCCCTCATTA 
    CACGCGGTGC TTGGACACAG TCATCTGAGA GAGGTGGATA CAAGTTGTGT TCTGTCTTGT 
    GGTGTGAACC CTAAGCAGGT TTACTATGTG CTAAACCCAG ATGGAAACCT GCCCGACACT 
    GAGAATCGCT TCAAAGAGTG GTTTACAGGT GAACGAACAT GGCAGGGGGT TTGTGGAAGG 
    GCTCCTGATC CAGAGAAACT ACAAGAGGCT GTGACCACTA AGGATCTCTA CATATACATC 
    GGACATGGTG CAGGAGCCCG CTTTCTGGAT GCGCAGAGAG TTTTGAAGGT GCCTGTGAGG 
    GCCGCAGCTC TTCTGTTCGG CTGCAGTAGT GCTGCTCTCA GTGTGCTTGG TCAGCAGGAG 
    GGAACGGGCA TCATCCTCAG CTACCTGACC GCTGGATGTC CATTGGTGCT GGGTAACCTG 
    TGGGATGTGA CAGATCGTGA CTTGGATCGA TTCACATCGG CTCTGCTCCA GTCTTGGCTC 
    TCTGCTGGTT CTGGCTCTTC ACTTCTTCAG CATTTGGCGA AATCACGCAG CGCAACTCAT 
    TTAAAACATA TAATCGGTGC TGCGCCCATT GCGTATGGTT TACCGGTGCA TATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327503.1
    CDS59..6478
    Translation

    Target ORF information:

    RefSeq Version XM_021471828.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021471828.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
    GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT 
    GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA 
    CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT 
    AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC 
    TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA 
    CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC 
    AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC 
    GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT 
    GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG 
    GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA 
    CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG 
    CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA 
    GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC 
    ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG 
    GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG 
    GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA 
    TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT 
    TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA 
    GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG 
    TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT 
    GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG 
    GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG 
    GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT 
    CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC 
    GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG 
    GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC 
    ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC 
    CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG 
    GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG 
    CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC 
    GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA 
    GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT 
    CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC 
    GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT 
    TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC 
    ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA 
    GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT 
    AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT 
    GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC 
    ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA 
    CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT 
    TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT 
    CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG 
    ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA 
    TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA 
    GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA 
    ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG 
    GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG 
    AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA 
    GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA 
    GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT 
    GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG 
    GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG 
    CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC 
    AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC 
    GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA 
    CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA 
    CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT 
    GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG 
    ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT 
    AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG 
    TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT 
    CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT 
    AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA 
    GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC 
    AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG 
    AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA 
    CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC 
    CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT 
    AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT 
    GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC 
    ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA 
    GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT 
    GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA 
    CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT 
    AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC 
    ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA 
    GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA 
    GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG CAGACTTGTC AGTAGAGACG 
    GTCCAGTCAT ACCTGCGCTC CTCATGGCTC CTCCTGCACC ACTTCCCGTT TCCTTCTCTT 
    TATCCTCACA TCTGCTCATT ACTCGCTCAG TCATTCGGTC AAACGGACCC CATCATCACA 
    GCAATGCTGC ATGCACAGGC ACTGGGAGTG TCCACTCGCC ACCATATGAC CCGTCACGTG 
    GTTAACCAGT TCAGGAAGTT GAAGAAATCC TGTAACGATG TAGCAGAAGG TCTTGGAGCT 
    CTGAGTCTGG AGGAAACATC TGGGGCAACG CAGTCAAAGA CACTCTCTGC GTTGGAGCAG 
    ATCTTTTCAT TCACAGCAGC AAACCCAATG CAGTTTCCAC AGACACACTG TCAGCAGTTC 
    ACACAGCAGC TGGAAGATCT ACCTGCAGGA GTGACCGTAT GCTTGCTGTC ACTGACGGGG 
    GTGCATCCTG ATGAAATTGG CAACACTATT CTTCTGACCC GACTGGAGCG CGGCTCTACC 
    CCAATCACAG TTCGAATCCC CACTGCAGAC AGAGAGCAAT CTGTTGCTGG CCTGTTGGAA 
    GAAATGGATG GAGTCTTGAA AGGACAAAAG GAAGTGAGCA CAGTGGCTGA GAAATCACAG 
    TGGTGGGACG GAAGGAAGGC TTTAGATGCC CGCGTGGAGA AAATGCTGGA GGAGATGGAA 
    GAGGCTTTAG GAGTGTGGCG AACTCTTCTT CTGCCTTTGA CCTCTGATCC TAAGCTTGAT 
    GTTCAGGTAA AGAGCCTCCA AAAGGCTCTT AAATCAACAA AGATCACACG GGACACTCTC 
    AAGGTGATTC TTTCAGCGTC TCCACTGCTG TCCCTGCCAG ACCTGCAGTG TCTTCTGGAA 
    GGAATGAATG TGCAGGACAA GGACCTGCTG AAGCTCCTGC AGAGGGCCGT GGCTGAACTG 
    AGAGGGAGGG AGGAGCAACA GGGACACACT GTTCTGATTT TAGACAAGTA CTTGCAGAGG 
    TTGCCGTGGG AGAACATTGC TTGTCTGAAG TCTCAGTCGG TCACTCGAAT GCCCTCATTA 
    CACGCGGTGC TTGGACACAG TCATCTGAGA GAGGTGGATA CAAGTTGTGT TCTGTCTTGT 
    GGTGTGAACC CTAAGCAGGT TTACTATGTG CTAAACCCAG ATGGAAACCT GCCCGACACT 
    GAGAATCGCT TCAAAGAGTG GTTTACAGGT GAACGAACAT GGCAGGGGGT TTGTGGAAGG 
    GCTCCTGATC CAGAGAAACT ACAAGAGGCT GTGACCACTA AGGATCTCTA CATATACATC 
    GGACATGGTG CAGGAGCCCG CTTTCTGGAT GCGCAGAGAG TTTTGAAGGT GCCTGTGAGG 
    GCCGCAGCTC TTCTGTTCGG CTGCAGTAGT GCTGCTCTCA GTGTGCTTGG TCAGCAGGAG 
    GGAACGGGCA TCATCCTCAG CTACCTGACC GCTGGATGTC CATTGGTGCT GGGTAACCTG 
    TGGGATGTGA CAGATCGTGA CTTGGATCGA TTCACATCGG CTCTGCTCCA GTCTTGGCTC 
    TCTGCTGGTT CTGGCTCTTC ACTTCTTCAG CATTTGGCGA AATCACGCAG CGCAACTCAT 
    TTAAAACATA TAATCGGTGC TGCGCCCATT GCGTATGGTT TACCGGTGCA TATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_021471829.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327504.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa144756
    Clone ID Related Accession (Same CDS sequence) XM_021471829.1
    Accession Version XM_021471829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product separin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
    GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT 
    GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA 
    CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT 
    AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC 
    TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA 
    CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC 
    AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC 
    GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT 
    GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG 
    GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA 
    CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG 
    CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA 
    GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC 
    ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG 
    GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG 
    GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA 
    TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT 
    TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA 
    GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG 
    TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT 
    GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG 
    GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG 
    GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT 
    CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC 
    GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG 
    GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC 
    ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC 
    CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG 
    GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG 
    CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC 
    GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA 
    GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT 
    CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC 
    GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT 
    TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC 
    ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA 
    GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT 
    AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT 
    GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC 
    ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA 
    CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT 
    TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT 
    CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG 
    ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA 
    TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA 
    GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA 
    ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG 
    GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG 
    AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA 
    GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA 
    GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT 
    GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG 
    GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG 
    CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC 
    AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC 
    GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA 
    CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA 
    CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT 
    GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG 
    ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT 
    AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG 
    TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT 
    CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT 
    AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA 
    GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC 
    AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG 
    AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA 
    CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC 
    CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT 
    AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT 
    GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC 
    ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA 
    GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT 
    GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA 
    CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT 
    AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC 
    ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA 
    GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA 
    GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG ACTTGTCAGT AGAGACGGTC 
    CAGTCATACC TGCGCTCCTC ATGGCTCCTC CTGCACCACT TCCCGTTTCC TTCTCTTTAT 
    CCTCACATCT GCTCATTACT CGCTCAGTCA TTCGGTCAAA CGGACCCCAT CATCACAGCA 
    ATGCTGCATG CACAGGCACT GGGAGTGTCC ACTCGCCACC ATATGACCCG TCACGTGGTT 
    AACCAGTTCA GGAAGTTGAA GAAATCCTGT AACGATGTAG CAGAAGGTCT TGGAGCTCTG 
    AGTCTGGAGG AAACATCTGG GGCAACGCAG TCAAAGACAC TCTCTGCGTT GGAGCAGATC 
    TTTTCATTCA CAGCAGCAAA CCCAATGCAG TTTCCACAGA CACACTGTCA GCAGTTCACA 
    CAGCAGCTGG AAGATCTACC TGCAGGAGTG ACCGTATGCT TGCTGTCACT GACGGGGGTG 
    CATCCTGATG AAATTGGCAA CACTATTCTT CTGACCCGAC TGGAGCGCGG CTCTACCCCA 
    ATCACAGTTC GAATCCCCAC TGCAGACAGA GAGCAATCTG TTGCTGGCCT GTTGGAAGAA 
    ATGGATGGAG TCTTGAAAGG ACAAAAGGAA GTGAGCACAG TGGCTGAGAA ATCACAGTGG 
    TGGGACGGAA GGAAGGCTTT AGATGCCCGC GTGGAGAAAA TGCTGGAGGA GATGGAAGAG 
    GCTTTAGGAG TGTGGCGAAC TCTTCTTCTG CCTTTGACCT CTGATCCTAA GCTTGATGTT 
    CAGGTAAAGA GCCTCCAAAA GGCTCTTAAA TCAACAAAGA TCACACGGGA CACTCTCAAG 
    GTGATTCTTT CAGCGTCTCC ACTGCTGTCC CTGCCAGACC TGCAGTGTCT TCTGGAAGGA 
    ATGAATGTGC AGGACAAGGA CCTGCTGAAG CTCCTGCAGA GGGCCGTGGC TGAACTGAGA 
    GGGAGGGAGG AGCAACAGGG ACACACTGTT CTGATTTTAG ACAAGTACTT GCAGAGGTTG 
    CCGTGGGAGA ACATTGCTTG TCTGAAGTCT CAGTCGGTCA CTCGAATGCC CTCATTACAC 
    GCGGTGCTTG GACACAGTCA TCTGAGAGAG GTGGATACAA GTTGTGTTCT GTCTTGTGGT 
    GTGAACCCTA AGCAGGTTTA CTATGTGCTA AACCCAGATG GAAACCTGCC CGACACTGAG 
    AATCGCTTCA AAGAGTGGTT TACAGGTGAA CGAACATGGC AGGGGGTTTG TGGAAGGGCT 
    CCTGATCCAG AGAAACTACA AGAGGCTGTG ACCACTAAGG ATCTCTACAT ATACATCGGA 
    CATGGTGCAG GAGCCCGCTT TCTGGATGCG CAGAGAGTTT TGAAGGTGCC TGTGAGGGCC 
    GCAGCTCTTC TGTTCGGCTG CAGTAGTGCT GCTCTCAGTG TGCTTGGTCA GCAGGAGGGA 
    ACGGGCATCA TCCTCAGCTA CCTGACCGCT GGATGTCCAT TGGTGCTGGG TAACCTGTGG 
    GATGTGACAG ATCGTGACTT GGATCGATTC ACATCGGCTC TGCTCCAGTC TTGGCTCTCT 
    GCTGGTTCTG GCTCTTCACT TCTTCAGCAT TTGGCGAAAT CACGCAGCGC AACTCATTTA 
    AAACATATAA TCGGTGCTGC GCCCATTGCG TATGGTTTAC CGGTGCATAT ACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327504.1
    CDS59..6475
    Translation

    Target ORF information:

    RefSeq Version XM_021471829.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021471829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
    GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT 
    GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA 
    CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT 
    AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC 
    TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA 
    CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC 
    AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC 
    GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT 
    GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG 
    GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA 
    CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG 
    CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA 
    GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC 
    ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG 
    GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG 
    GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA 
    TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT 
    TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA 
    GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG 
    TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT 
    GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG 
    GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG 
    GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT 
    CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC 
    GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG 
    GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC 
    ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC 
    CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG 
    GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG 
    CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC 
    GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA 
    GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT 
    CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC 
    GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT 
    TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC 
    ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA 
    GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT 
    AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT 
    GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC 
    ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA 
    CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT 
    TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT 
    CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG 
    ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA 
    TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA 
    GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA 
    ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG 
    GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG 
    AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA 
    GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA 
    GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT 
    GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG 
    GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG 
    CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC 
    AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC 
    GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA 
    CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA 
    CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT 
    GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG 
    ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT 
    AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG 
    TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT 
    CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT 
    AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA 
    GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC 
    AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG 
    AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA 
    CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC 
    CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT 
    AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT 
    GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC 
    ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA 
    GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT 
    GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA 
    CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT 
    AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC 
    ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA 
    GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA 
    GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG ACTTGTCAGT AGAGACGGTC 
    CAGTCATACC TGCGCTCCTC ATGGCTCCTC CTGCACCACT TCCCGTTTCC TTCTCTTTAT 
    CCTCACATCT GCTCATTACT CGCTCAGTCA TTCGGTCAAA CGGACCCCAT CATCACAGCA 
    ATGCTGCATG CACAGGCACT GGGAGTGTCC ACTCGCCACC ATATGACCCG TCACGTGGTT 
    AACCAGTTCA GGAAGTTGAA GAAATCCTGT AACGATGTAG CAGAAGGTCT TGGAGCTCTG 
    AGTCTGGAGG AAACATCTGG GGCAACGCAG TCAAAGACAC TCTCTGCGTT GGAGCAGATC 
    TTTTCATTCA CAGCAGCAAA CCCAATGCAG TTTCCACAGA CACACTGTCA GCAGTTCACA 
    CAGCAGCTGG AAGATCTACC TGCAGGAGTG ACCGTATGCT TGCTGTCACT GACGGGGGTG 
    CATCCTGATG AAATTGGCAA CACTATTCTT CTGACCCGAC TGGAGCGCGG CTCTACCCCA 
    ATCACAGTTC GAATCCCCAC TGCAGACAGA GAGCAATCTG TTGCTGGCCT GTTGGAAGAA 
    ATGGATGGAG TCTTGAAAGG ACAAAAGGAA GTGAGCACAG TGGCTGAGAA ATCACAGTGG 
    TGGGACGGAA GGAAGGCTTT AGATGCCCGC GTGGAGAAAA TGCTGGAGGA GATGGAAGAG 
    GCTTTAGGAG TGTGGCGAAC TCTTCTTCTG CCTTTGACCT CTGATCCTAA GCTTGATGTT 
    CAGGTAAAGA GCCTCCAAAA GGCTCTTAAA TCAACAAAGA TCACACGGGA CACTCTCAAG 
    GTGATTCTTT CAGCGTCTCC ACTGCTGTCC CTGCCAGACC TGCAGTGTCT TCTGGAAGGA 
    ATGAATGTGC AGGACAAGGA CCTGCTGAAG CTCCTGCAGA GGGCCGTGGC TGAACTGAGA 
    GGGAGGGAGG AGCAACAGGG ACACACTGTT CTGATTTTAG ACAAGTACTT GCAGAGGTTG 
    CCGTGGGAGA ACATTGCTTG TCTGAAGTCT CAGTCGGTCA CTCGAATGCC CTCATTACAC 
    GCGGTGCTTG GACACAGTCA TCTGAGAGAG GTGGATACAA GTTGTGTTCT GTCTTGTGGT 
    GTGAACCCTA AGCAGGTTTA CTATGTGCTA AACCCAGATG GAAACCTGCC CGACACTGAG 
    AATCGCTTCA AAGAGTGGTT TACAGGTGAA CGAACATGGC AGGGGGTTTG TGGAAGGGCT 
    CCTGATCCAG AGAAACTACA AGAGGCTGTG ACCACTAAGG ATCTCTACAT ATACATCGGA 
    CATGGTGCAG GAGCCCGCTT TCTGGATGCG CAGAGAGTTT TGAAGGTGCC TGTGAGGGCC 
    GCAGCTCTTC TGTTCGGCTG CAGTAGTGCT GCTCTCAGTG TGCTTGGTCA GCAGGAGGGA 
    ACGGGCATCA TCCTCAGCTA CCTGACCGCT GGATGTCCAT TGGTGCTGGG TAACCTGTGG 
    GATGTGACAG ATCGTGACTT GGATCGATTC ACATCGGCTC TGCTCCAGTC TTGGCTCTCT 
    GCTGGTTCTG GCTCTTCACT TCTTCAGCAT TTGGCGAAAT CACGCAGCGC AACTCATTTA 
    AAACATATAA TCGGTGCTGC GCCCATTGCG TATGGTTTAC CGGTGCATAT ACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_021474123.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329798.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa146569
    Clone ID Related Accession (Same CDS sequence) XM_021474123.1
    Accession Version XM_021474123.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG 
    GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT 
    CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT 
    CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG 
    AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA 
    ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT 
    GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA 
    TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG 
    TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT 
    GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC 
    GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA 
    TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC 
    ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC 
    TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT 
    AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG 
    ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC 
    TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA 
    GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT 
    GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGCAGA CTTGTCAGTA 
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CTGTATGCTT GCTGTCACTG 
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTCTTCTGC CTTTGACCTC TGATCCTAAG 
    CTTGATGTAC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACAGGAT 
    ACTCTCAAGG TGATTCTTTC AGCCTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTTCAGAG AGGCGTGGCT 
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
    TCATTACATG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTG 
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
    GTGAGGGCCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCAAT GGTGCTGGGT 
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329798.1
    CDS221..6766
    Translation

    Target ORF information:

    RefSeq Version XM_021474123.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021474123.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG 
    GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT 
    CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT 
    CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG 
    AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA 
    ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT 
    GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA 
    TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG 
    TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT 
    GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC 
    GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA 
    TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC 
    ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC 
    TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT 
    AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG 
    ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC 
    TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA 
    GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT 
    GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGCAGA CTTGTCAGTA 
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CTGTATGCTT GCTGTCACTG 
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTCTTCTGC CTTTGACCTC TGATCCTAAG 
    CTTGATGTAC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACAGGAT 
    ACTCTCAAGG TGATTCTTTC AGCCTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTTCAGAG AGGCGTGGCT 
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
    TCATTACATG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTG 
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
    GTGAGGGCCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCAAT GGTGCTGGGT 
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    espl1 ( XM_021474124.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329799.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa146570
    Clone ID Related Accession (Same CDS sequence) XM_021474124.1
    Accession Version XM_021474124.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product separin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG 
    GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT 
    CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT 
    CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG 
    AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA 
    ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT 
    GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA 
    TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG 
    TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT 
    GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC 
    GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA 
    TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC 
    ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC 
    TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT 
    AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG 
    ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC 
    TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA 
    GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT 
    GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGACTT GTCAGTAGAG 
    ACGGTCCAGT CATACCTGCG CTCCTCATGG CTCCTCCTGC ACCACTTCCC GTTTCCTTCT 
    CTTTATCCTC ACATCTGCTC ATTACTCGCT CAGTCATTCG GTCAAACGGA CCCCATCATC 
    ACAGCAATGC TGCATGCACA GGCACTGGGA GTGTCCACTC GCCACCATAT GACCCGTCAC 
    GTGGTTAACC AGTTCAGAAA GTTGAAGAAA TCCTGTAACG ATGTAGCAGA AGGTCTTGGA 
    GCTCTGAGTC TGGAGGAAAC ATCTGGAGCA ACGCAGTCAA AGAAACTCTC TGCGTTGGAG 
    CAGATCTTTT CATTCACAGC ATCAAACCCA ATGCAGTTTC CACAGACACA CTGTCAGCAG 
    TTCACACAGC AGCTGGAAGA TCTACCTGCA GGAGTGACTG TATGCTTGCT GTCACTGACG 
    GGGGTGCATC CTGATGAAAT TGGCAACACT ATTCTTCTGA CCCGACTGGA GCGCGGCTCT 
    ACCCCAATCA CAGTTCGAAT CCCCACTGCA GACAGAGAGC AATCTGTTGC TGGCCTGTTG 
    GAAGAAATGG ATGGAGTCTT GAAAGGACAA AAGGAAGTGA GCACAGTGGC TGAGAAATCA 
    CAGTGGTGGG ACGGAAGGAA GGCTTTAGAT GCCCGTGTGG AGAAAATGCT GGAGGAGATG 
    GAAGAGGCTT TAGGAGTGTG GCGAACTCTT CTTCTGCCTT TGACCTCTGA TCCTAAGCTT 
    GATGTACAGG TAAAGAGCCT CCAAAAGGCT CTTAAATCAA CAAAGATCAC ACAGGATACT 
    CTCAAGGTGA TTCTTTCAGC CTCTCCACTG CTGTCCCTGC CAGACCTGCA GTGTCTTCTG 
    GAAGGAATGA ATGTGCAGGA CAAGGACCTG CTGAAGCTCC TTCAGAGAGG CGTGGCTGAA 
    CTGAGAGGGA GGGAGGAGCC ACAGGGACAC ACTGTTCTGA TTTTAGACAA GTACTTGCAG 
    AGGTTGCCGT GGGAGAACAT TGCTTGTCTG AAGTCTCAGT CGGTCACTCG AATGCCCTCA 
    TTACATGCGG TGCTTGGACA CAGTCATCTG AGAGAGGTGG ATACAAGTTG TGTTCTGTCT 
    TGTGGTGTGA ACCCTAAGCA GGTTTACTAT GTGCTAAACC CAGATGGAAA CCTGCCCGAC 
    ACTGAGAATC GCTTCAAAGA GTGGTTTACA GGTGAACGAA CATGGCAGGG GGTTTGTGGA 
    AGGGCTCCTG ATCCGGAGAA ACTACAAGAG GCTGTGACCA CTAAAGATCT CTACATATAC 
    ATCGGACATG GTGCAGGAGC CCGCTTTCTG GATGCGCAGA GAGTTTTGAA GGTGCCTGTG 
    AGGGCCGCAG CTCTTCTGTT CGGCTGCAGT AGTGCTGCTC TCAGTGTGCT TGGTCAGCAG 
    GAGGGAACGG GCATCATCCT CAGCTACCTG ACCGCTGGAT GTCCAATGGT GCTGGGTAAC 
    CTGTGGGATG TGACAGATCG TGACTTGGAT CGATTCACAT CGGCTCTGCT CCAGTCTTGG 
    CTCTCTGCTG GTTCTGGCTC TTCACTTCTT CAGCATTTGG CGAAATCACG CAGCGCAACT 
    CATTTAAAAC ACATAATCGG TGCTGCGCCC ATTGCGTATG GTTTACCGGT GCATATACAC 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329799.1
    CDS222..6764
    Translation

    Target ORF information:

    RefSeq Version XM_021474124.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021474124.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG 
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
    ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG 
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG 
    GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG 
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
    TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC 
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
    TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
    ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
    TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG 
    GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT 
    CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
    CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
    CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
    GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
    GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC 
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
    AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT 
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC 
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT 
    CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG 
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT 
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
    CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT 
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG 
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
    CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG 
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG 
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
    CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
    GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC 
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG 
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA 
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT 
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT 
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG 
    AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA 
    ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT 
    GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA 
    TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG 
    TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT 
    GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC 
    GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA 
    TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC 
    ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC 
    TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT 
    AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG 
    ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC 
    TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA 
    GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT 
    GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGACTT GTCAGTAGAG 
    ACGGTCCAGT CATACCTGCG CTCCTCATGG CTCCTCCTGC ACCACTTCCC GTTTCCTTCT 
    CTTTATCCTC ACATCTGCTC ATTACTCGCT CAGTCATTCG GTCAAACGGA CCCCATCATC 
    ACAGCAATGC TGCATGCACA GGCACTGGGA GTGTCCACTC GCCACCATAT GACCCGTCAC 
    GTGGTTAACC AGTTCAGAAA GTTGAAGAAA TCCTGTAACG ATGTAGCAGA AGGTCTTGGA 
    GCTCTGAGTC TGGAGGAAAC ATCTGGAGCA ACGCAGTCAA AGAAACTCTC TGCGTTGGAG 
    CAGATCTTTT CATTCACAGC ATCAAACCCA ATGCAGTTTC CACAGACACA CTGTCAGCAG 
    TTCACACAGC AGCTGGAAGA TCTACCTGCA GGAGTGACTG TATGCTTGCT GTCACTGACG 
    GGGGTGCATC CTGATGAAAT TGGCAACACT ATTCTTCTGA CCCGACTGGA GCGCGGCTCT 
    ACCCCAATCA CAGTTCGAAT CCCCACTGCA GACAGAGAGC AATCTGTTGC TGGCCTGTTG 
    GAAGAAATGG ATGGAGTCTT GAAAGGACAA AAGGAAGTGA GCACAGTGGC TGAGAAATCA 
    CAGTGGTGGG ACGGAAGGAA GGCTTTAGAT GCCCGTGTGG AGAAAATGCT GGAGGAGATG 
    GAAGAGGCTT TAGGAGTGTG GCGAACTCTT CTTCTGCCTT TGACCTCTGA TCCTAAGCTT 
    GATGTACAGG TAAAGAGCCT CCAAAAGGCT CTTAAATCAA CAAAGATCAC ACAGGATACT 
    CTCAAGGTGA TTCTTTCAGC CTCTCCACTG CTGTCCCTGC CAGACCTGCA GTGTCTTCTG 
    GAAGGAATGA ATGTGCAGGA CAAGGACCTG CTGAAGCTCC TTCAGAGAGG CGTGGCTGAA 
    CTGAGAGGGA GGGAGGAGCC ACAGGGACAC ACTGTTCTGA TTTTAGACAA GTACTTGCAG 
    AGGTTGCCGT GGGAGAACAT TGCTTGTCTG AAGTCTCAGT CGGTCACTCG AATGCCCTCA 
    TTACATGCGG TGCTTGGACA CAGTCATCTG AGAGAGGTGG ATACAAGTTG TGTTCTGTCT 
    TGTGGTGTGA ACCCTAAGCA GGTTTACTAT GTGCTAAACC CAGATGGAAA CCTGCCCGAC 
    ACTGAGAATC GCTTCAAAGA GTGGTTTACA GGTGAACGAA CATGGCAGGG GGTTTGTGGA 
    AGGGCTCCTG ATCCGGAGAA ACTACAAGAG GCTGTGACCA CTAAAGATCT CTACATATAC 
    ATCGGACATG GTGCAGGAGC CCGCTTTCTG GATGCGCAGA GAGTTTTGAA GGTGCCTGTG 
    AGGGCCGCAG CTCTTCTGTT CGGCTGCAGT AGTGCTGCTC TCAGTGTGCT TGGTCAGCAG 
    GAGGGAACGG GCATCATCCT CAGCTACCTG ACCGCTGGAT GTCCAATGGT GCTGGGTAAC 
    CTGTGGGATG TGACAGATCG TGACTTGGAT CGATTCACAT CGGCTCTGCT CCAGTCTTGG 
    CTCTCTGCTG GTTCTGGCTC TTCACTTCTT CAGCATTTGG CGAAATCACG CAGCGCAACT 
    CATTTAAAAC ACATAATCGG TGCTGCGCCC ATTGCGTATG GTTTACCGGT GCATATACAC 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission