espl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	espl1
Entrez Gene ID	100158636
Full Name	extra spindle pole bodies like 1, separase
General protein information	Preferred Names extra spindle pole bodies like 1, separase Names separin extra spindle pole bodies homolog 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004911948.3	XP_004912005.1	separin
XM_004911948.4	XP_004912005.2	separin
XM_031897204.1	XP_031753064.1	separin
XM_031897205.1	XP_031753065.1	separin

Pathways from BioSystems

Homologies

Homo sapiens (human)	ESPL1	NP_036423.4
Macaca mulatta (Rhesus monkey)	ESPL1	XP_001103494.1
Danio rerio (zebrafish)	espl1	XP_001337869.1
Pan troglodytes (chimpanzee)	ESPL1	XP_003313754.1
Canis lupus familiaris (dog)	ESPL1	XP_849828.2
Bos taurus (cattle)	ESPL1	NP_001039414.2
Gallus gallus (chicken)	ESPL1	XP_423289.4
Mus musculus (house mouse)	Espl1	NP_001014976.1
Rattus norvegicus (Norway rat)	Espl1	NP_001164073.1
Xenopus tropicalis (tropical clawed frog)	espl1	XP_004912005.1

Gene Ontology

The following espl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the espl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa06113	XM_004911948.3 Latest version!	Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa52740	XM_004911948.4 Latest version!	Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa52740	XM_031897204.1 Latest version!	Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa52740	XM_031897205.1 Latest version!	Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

espl1 ( XM_004911948.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912005.1 Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), mRNA.

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CloneID	OXa06113
Clone ID Related Accession (Same CDS sequence)	XM_004911948.3
Accession Version	XM_004911948.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	separin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911948.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCATTT 
GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT 
CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA 
GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA 
TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG 
TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG 
TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT 
TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA 
CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG 
CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA 
CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA 
GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT 
GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC 
AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA 
TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG 
GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA 
ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT 
TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT 
GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA 
GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC 
AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG 
GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC 
AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC 
TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC 
CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG 
GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT 
CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC 
TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA 
AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG 
GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT 
GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG 
GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT 
TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT 
CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT 
CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA 
AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC 
GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT 
ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC 
AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA 
TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC 
TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GGTTGGGGCT 
CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC 
CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG 
GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG 
GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG 
GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA 
TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT 
GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA 
AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA 
AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG 
AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC 
AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA 
GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG 
TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA 
AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC 
CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA 
AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG 
TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC 
ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA 
GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT 
GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG 
TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA 
CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT 
CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA 
GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC 
CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA 
CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC 
ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC 
CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA 
AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC 
TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA 
CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA 
CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC 
ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG 
ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC 
TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA 
CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA 
GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA 
GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC 
AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT 
CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA 
AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG 
CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA 
GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG 
CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG 
TTCCAAGAGC AACTACACCA GATCCCTGCA AATACAACAA TGTGTATATT GACCTTGGTG 
GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA 
CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG 
CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG 
GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA 
GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT 
GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA 
GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT 
CAGTCCTTCA TCCACGGATT TGGATTTTTA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA 
AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG 
CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA 
GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG 
CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT 
CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG 
TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG 
AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA 
CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT 
GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC 
CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA 
GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA 
GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC 
TTACCAGTGG CCCTGCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912005.1
CDS	117..6497
Translation	MAGNLKSLKGADFIKLTSSFEEVTLLHSKLKLCLSEVTADQLPDQRVVCDRVLRACNHWAGDPNCHVSHWQSLVPLAALVCQGFCASSPQRTPFYLEKILFHLLKNISARVSSSHACSNLSDLLYGLLTGCPLEQQSAGDFTAVSKSSFQILWKSAGDQEQKQSPSSCNREVLSLRLHALRFLMLAEGSVSHGDAPQTISRHARCAALAFEPKGKPCSKEETYFLSQTLEDLLVKPLLGRGTLSGSWSRCLVELTLERCRRLCKTCCFKQGRHVVEQARGCLEKTERCCHAGLALLTFAVDIHESGIPPSIGEVLSEVIETLDASQSCGDGWMCQALSECFQFAVSSLDGRFKHSGKLKVDDILSLAKFLEKHHQLLEKQVTKVPKDVTRQLKSLKQQQFNSLQQFTNAACAFMQSCKDKAKDGLLLLLGACKDAVSRMHSSLQGLPTQETTEFLNITASCVHNLAYWFYSQKMHQEASDLVYPLNAWLAQSAPDGDPDLAVERLHRCYRLHVESCRKSGQIMKGLEAVGLWLRALRSQVSQHMAEPVALWVRLKMDGAKSGDEDVRLHTLKDGLVDQHVDPELMVSLLAEELQGYKSVHGDTGQERYNTICDLLELCHEDSDLRGHRAIFLLELAQVLCYHDYTGQTDCSALDAVQEALRLLNATSKPTERLQDIKAQALLWQYICTLEANMREGLEEQNRKAKLEGQNQWTGVDYEPNDLNYEDKLHDDQSVRDGICFTLAGETGPLKGLDEALDLWSSLLSSSQVQYLNSAEQTIFSLHLLGSLYRLMGKPLQSMQSFQLAGRLCHSLQDHIKEVGALCHLTKILFYLESPEYAQVILQKAEEILKRADRTSENYTLAELSCQLLRSHLCRVTRQVKEGVELLLGLLQHPSLQKSSKAWYLLKVQVLQELSLFLMLPLENLTSDLYRQLWAHGCHNPETALTEAHKLLRSIVLLLLGNDVLASPKGSSEVRFVDNGENLLQKWQVLADLLSCTHDLVKTLGRMGCVSEAKSFCLEGLRVAKTLQSMRHCADFLVRKAELETLRSETQLCEEDLQYVLFLMESCTDFAAKSKQKEVKIKLCKGKPFHKVDITETPPSPPKDDFLKAVDLHYVETRDLSKAPESPTCKPSLEKNLPHFLSHTQDCTCPLCSDLVLSLLCTQWLVAKAENNTQSRGLLKSALKRCKALTQRFSGIVQEILGKGVTKPAVLGVASELMARVYLAMASQSPSKLPEETLEEGLSFVSSRQATLPKHWRAGLLLAKALCSIYNLAAKHGGCVADMFVQLWGWQPHQNGKKAADLNHRNKTTASITKKKTKKMSLDVPSSFPFEDSDTEPPKPHTTPTQKNLPATEARSVTSTLPKKSSITVYNEESPMPEVLPRAPRRRKTRTVLKVDFSDSDLEVPDNPEKEPVSNCRKQAVQTKSAGRGRGRGRSSVAQAPLDDSDDDVTNKSKTRKNRPSTRRGQKESSPTRPVRRRQQVTMDVAPKEVDVLRSIEEEPDWTLDVSFEELRGSDTEQKKTLRTGRKGQKSKNEGLVTECEVLRRDAGGEHLCWTPNMIGGETDLNPFSIAGNSSSLPATFSASDLSLVSSLLHEALESIAHFPPSTLYSRLCRLLALCRGRQDPYVTALLVSESVSITLRHQLLSDIHRKLRKIRREGSGDVCDKFQDLTLEDHKDAQVQHLSQLEEIFQFPKQALDVQRFQEQLHQIPANTTMCILTLVDSQTRMPGDTILLTRLVRGRPPVTVQIPTAHLKVSISSTLQEFDEIQKQQKAVNNLTDKKEWWEGRMELDCRMKALIRLLEEQILGCWKVLLSCPCSSDAVIEECDRLSQTLSDSGWGRTDPELLKILLSCCHFLTPHLIQSFIHGFGFLKPEQAQELVQSAAERLKAGTEQSEGHLVLVLDKHLQKLPWESIPCLQKQSVTRLPSLRFLLSYGLQRKYQPETTLMKGVDPKQAFYVLNPHANLPGTEERFRDWFKNEPGWKGVIRSAPKSEMIQSALTKKDLYIYVGHGAGAHFLDVQTLQRLHCNAVVLLFGCSSVALAVRGDLEGAGIVLKYLMAGCPLVLGNLWDVTDREIDRYTVAFLQSWLKAGSGAPLLKYLSESRQAPKLKYIIGAAPVAYGLPVALR

Target ORF information:

RefSeq Version	XM_004911948.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911948.3

ORF Insert Sequence:

ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCATTT 
GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT 
CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA 
GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA 
TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG 
TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG 
TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT 
TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA 
CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG 
CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA 
CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA 
GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT 
GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC 
AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA 
TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG 
GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA 
ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT 
TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT 
GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA 
GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC 
AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG 
GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC 
AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC 
TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC 
CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG 
GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT 
CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC 
TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA 
AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG 
GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT 
GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG 
GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT 
TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT 
CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT 
CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA 
AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC 
GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT 
ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC 
AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA 
TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC 
TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GGTTGGGGCT 
CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC 
CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG 
GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG 
GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG 
GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA 
TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT 
GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA 
AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA 
AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG 
AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC 
AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA 
GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG 
TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA 
AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC 
CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA 
AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG 
TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC 
ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA 
GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT 
GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG 
TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA 
CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT 
CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA 
GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC 
CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA 
CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC 
ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC 
CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA 
AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC 
TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA 
CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA 
CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC 
ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG 
ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC 
TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA 
CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA 
GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA 
GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC 
AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT 
CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA 
AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG 
CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA 
GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG 
CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG 
TTCCAAGAGC AACTACACCA GATCCCTGCA AATACAACAA TGTGTATATT GACCTTGGTG 
GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA 
CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG 
CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG 
GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA 
GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT 
GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA 
GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT 
CAGTCCTTCA TCCACGGATT TGGATTTTTA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA 
AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG 
CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA 
GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG 
CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT 
CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG 
TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG 
AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA 
CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT 
GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC 
CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA 
GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA 
GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC 
TTACCAGTGG CCCTGCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_004911948.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912005.2 Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

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CloneID	OXa52740
Clone ID Related Accession (Same CDS sequence)	XM_004911948.4 , XM_031897204.1 , XM_031897205.1
Accession Version	XM_004911948.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	separin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911948.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT 
CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA 
GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA 
TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG 
TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG 
TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT 
TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA 
CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG 
CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA 
CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA 
GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT 
GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC 
AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA 
TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG 
GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA 
ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT 
TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT 
GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA 
GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC 
AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG 
GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC 
AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC 
TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC 
CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG 
GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT 
CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC 
TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA 
AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG 
GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT 
GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG 
GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT 
TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT 
CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT 
CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA 
AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC 
GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT 
ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC 
AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA 
TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC 
TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT 
CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC 
CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG 
GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG 
GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG 
GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA 
TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT 
GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA 
AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA 
AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG 
AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC 
AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA 
GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG 
TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA 
AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC 
CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA 
AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG 
TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC 
ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA 
GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT 
GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG 
TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA 
CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT 
CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA 
GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC 
CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA 
CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC 
ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC 
CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA 
AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC 
TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA 
CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA 
CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC 
ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG 
ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC 
TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA 
CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA 
GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA 
GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC 
AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT 
CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA 
AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG 
CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA 
GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG 
CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG 
TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG 
GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA 
CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG 
CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG 
GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA 
GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT 
GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA 
GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT 
CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA 
AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG 
CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA 
GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG 
CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT 
CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG 
TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG 
AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA 
CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT 
GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC 
CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA 
GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA 
GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC 
TTACCAGTGG CCCTGCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912005.2
CDS	116..6496
Translation	MAGNLKSLKGADFIKLTSSVEEVTLLHSKLKLCLSEVTADQLPDQRVVCDRVLRACNHWAGDPNCHVSHWQSLVPLAALVCQGFCASSPQRTPFYLEKILFHLLKNISARVSSSHACSNLSDLLYGLLTGCPLEQQSAGDFTAVSKSSFQILWKSAGDQEQKQSPSSCNREVLSLRLHALRFLMLAEGSVSHGDAPQTISRHARCAALAFEPKGKPCSKEETYFLSQTLEDLLVKPLLGRGTLSGSWSRCLVELTLERCRRLCKTCCFKQGRHVVEQARGCLEKTERCCHAGLALLTFAVDIHESGIPPSIGEVLSEVIETLDASQSCGDGWMCQALSECFQFAVSSLDGRFKHSGKLKVDDILSLAKFLEKHHQLLEKQVTKVPKDVTRQLKSLKQQQFNSLQQFTNAACAFMQSCKDKAKDGLLLLLGACKDAVSRMHSSLQGLPTQETTEFLNITASCVHNLAYWFYSQKMHQEASDLVYPLNAWLAQSAPDGDPDLAVERLHRCYRLHVESCRKSGQIMKGLEAVGLWLRALRSQVSQHMAEPVALWVRLKMDGAKSGDEDVRLHTLKDGLVDQHVDPELMVSLLAEELQGYKSVHGDTGQERYNTICDLLELCHEDSDLRGHRAIFLLELAQVLCYHDYTGQTDCSALDAVQEALRLLNATSKPTERLQDIKAQALLWQYICTLEANMREGLEEQNRKAKLEGQNQWTGVDYEPNDLNYEDKLHDDQSVRDGICFTLAGETGPLKGLDEALDLWSSLLSSSQVQYLNSAEQTIFSLHLLGSLYRLMGKPLQSMQSFQLAGRLCHSLQDHIKEIGALCHLTKILFYLESPEYAQVILQKAEEILKRADRTSENYTLAELSCQLLRSHLCRVTRQVKEGVELLLGLLQHPSLQKSSKAWYLLKVQVLQELSLFLMLPLENLTSDLYRQLWAHGCHNPETALTEAHKLLRSIVLLLLGNDVLASPKGSSEVRFVDNGENLLQKWQVLADLLSCTHDLVKTLGRMGCVSEAKSFCLEGLRVAKTLQSMRHCADFLVRKAELETLRSETQLCEEDLQYVLFLMESCTDFAAKSKQKEVKIKLCKGKPFHKVDITETPPSPPKDDFLKAVDLHYVETRDLSKAPESPTCKPSLEKNLPHFLSHTQDCTCPLCSDLVLSLLCTQWLVAKAENNTQSRGLLKSALKRCKALTQRFSGIVQEILGKGVTKPAVLGVASELMARVYLAMASQSPSKLPEETLEEGLSFVSSRQATLPKHWRAGLLLAKALCSIYNLAAKHGGCVADMFVQLWGWQPHQNGKKAADLNHRNKTTASITKKKTKKMSLDVPSSFPFEDSDTEPPKPHTTPTQKNLPATEARSVTSTLPKKSSITVYNEESPMPEVLPRAPRRRKTRTVLKVDFSDSDLEVPDNPEKEPVSNCRKQAVQTKSAGRGRGRGRSSVAQAPLDDSDDDVTNKSKTRKNRPSTRRGQKESSPTRPVRRRQQVTMDVAPKEVDVLRSIEEEPDWTLDVSFEELRGSDTEQKKTLRTGRKGQKSKNEGLVTECEVLRRDAGGEHLCWTPNMIGGETDLNPFSIAGNSSSLPATFSASDLSLVSSLLHEALESIAHFPPSTLYSRLCRLLALCRGRQDPYVTALLVSESVSITLRHQLLSDIHRKLRKIRREGSGDVCDKFQDLTLEDHKDAQVQHLSQLEEIFQFPKQALDVQRFQEQLHQIPANTTMCILTLVDSQTRMPGDTILLTRLVRGRPPVTVQIPTAHLKVSISSTLQEFDEIQKQQKAVNNLTDKKEWWEGRMELDCRMKALIRLLEEQILGCWKVLLSCPCSSDAVIEECDRLSQTLSDSGWGRTDPELLKILLSCCHFLTPHLIQSFIHGFGFSKPEQAQELVQSAAERLKAGTEQSEGHLVLVLDKHLQKLPWESIPCLQKQSVTRLPSLRFLLSYGLQRKYQPETTLMKGVDPKQAFYVLNPHANLPGTEERFRDWFKNEPGWKGVIRSAPKSEMIQSALTKKDLYIYVGHGAGAHFLDVQTLQRLHCNAVVLLFGCSSVALAVRGDLEGAGIVLKYLMAGCPLVLGNLWDVTDREIDRYTVAFLQSWLKAGSGAPLLKYLSESRQAPKLKYIIGAAPVAYGLPVALR

Target ORF information:

RefSeq Version	XM_004911948.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911948.4

ORF Insert Sequence:

ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT 
CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA 
GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA 
TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG 
TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG 
TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT 
TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA 
CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG 
CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA 
CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA 
GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT 
GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC 
AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA 
TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG 
GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA 
ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT 
TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT 
GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA 
GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC 
AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG 
GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC 
AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC 
TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC 
CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG 
GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT 
CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC 
TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA 
AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG 
GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT 
GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG 
GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT 
TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT 
CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT 
CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA 
AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC 
GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT 
ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC 
AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA 
TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC 
TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT 
CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC 
CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG 
GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG 
GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG 
GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA 
TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT 
GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA 
AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA 
AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG 
AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC 
AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA 
GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG 
TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA 
AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC 
CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA 
AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG 
TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC 
ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA 
GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT 
GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG 
TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA 
CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT 
CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA 
GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC 
CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA 
CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC 
ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC 
CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA 
AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC 
TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA 
CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA 
CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC 
ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG 
ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC 
TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA 
CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA 
GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA 
GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC 
AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT 
CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA 
AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG 
CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA 
GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG 
CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG 
TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG 
GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA 
CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG 
CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG 
GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA 
GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT 
GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA 
GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT 
CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA 
AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG 
CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA 
GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG 
CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT 
CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG 
TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG 
AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA 
CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT 
GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC 
CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA 
GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA 
GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC 
TTACCAGTGG CCCTGCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_031897204.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753064.1 Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

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CloneID	OXa52740
Clone ID Related Accession (Same CDS sequence)	XM_004911948.4 , XM_031897204.1 , XM_031897205.1
Accession Version	XM_031897204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	separin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT 
CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA 
GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA 
TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG 
TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG 
TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT 
TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA 
CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG 
CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA 
CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA 
GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT 
GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC 
AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA 
TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG 
GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA 
ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT 
TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT 
GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA 
GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC 
AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG 
GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC 
AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC 
TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC 
CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG 
GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT 
CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC 
TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA 
AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG 
GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT 
GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG 
GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT 
TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT 
CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT 
CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA 
AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC 
GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT 
ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC 
AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA 
TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC 
TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT 
CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC 
CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG 
GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG 
GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG 
GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA 
TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT 
GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA 
AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA 
AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG 
AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC 
AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA 
GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG 
TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA 
AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC 
CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA 
AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG 
TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC 
ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA 
GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT 
GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG 
TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA 
CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT 
CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA 
GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC 
CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA 
CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC 
ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC 
CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA 
AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC 
TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA 
CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA 
CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC 
ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG 
ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC 
TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA 
CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA 
GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA 
GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC 
AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT 
CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA 
AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG 
CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA 
GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG 
CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG 
TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG 
GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA 
CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG 
CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG 
GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA 
GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT 
GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA 
GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT 
CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA 
AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG 
CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA 
GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG 
CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT 
CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG 
TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG 
AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA 
CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT 
GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC 
CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA 
GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA 
GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC 
TTACCAGTGG CCCTGCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753064.1
CDS	103..6483
Translation	MAGNLKSLKGADFIKLTSSVEEVTLLHSKLKLCLSEVTADQLPDQRVVCDRVLRACNHWAGDPNCHVSHWQSLVPLAALVCQGFCASSPQRTPFYLEKILFHLLKNISARVSSSHACSNLSDLLYGLLTGCPLEQQSAGDFTAVSKSSFQILWKSAGDQEQKQSPSSCNREVLSLRLHALRFLMLAEGSVSHGDAPQTISRHARCAALAFEPKGKPCSKEETYFLSQTLEDLLVKPLLGRGTLSGSWSRCLVELTLERCRRLCKTCCFKQGRHVVEQARGCLEKTERCCHAGLALLTFAVDIHESGIPPSIGEVLSEVIETLDASQSCGDGWMCQALSECFQFAVSSLDGRFKHSGKLKVDDILSLAKFLEKHHQLLEKQVTKVPKDVTRQLKSLKQQQFNSLQQFTNAACAFMQSCKDKAKDGLLLLLGACKDAVSRMHSSLQGLPTQETTEFLNITASCVHNLAYWFYSQKMHQEASDLVYPLNAWLAQSAPDGDPDLAVERLHRCYRLHVESCRKSGQIMKGLEAVGLWLRALRSQVSQHMAEPVALWVRLKMDGAKSGDEDVRLHTLKDGLVDQHVDPELMVSLLAEELQGYKSVHGDTGQERYNTICDLLELCHEDSDLRGHRAIFLLELAQVLCYHDYTGQTDCSALDAVQEALRLLNATSKPTERLQDIKAQALLWQYICTLEANMREGLEEQNRKAKLEGQNQWTGVDYEPNDLNYEDKLHDDQSVRDGICFTLAGETGPLKGLDEALDLWSSLLSSSQVQYLNSAEQTIFSLHLLGSLYRLMGKPLQSMQSFQLAGRLCHSLQDHIKEIGALCHLTKILFYLESPEYAQVILQKAEEILKRADRTSENYTLAELSCQLLRSHLCRVTRQVKEGVELLLGLLQHPSLQKSSKAWYLLKVQVLQELSLFLMLPLENLTSDLYRQLWAHGCHNPETALTEAHKLLRSIVLLLLGNDVLASPKGSSEVRFVDNGENLLQKWQVLADLLSCTHDLVKTLGRMGCVSEAKSFCLEGLRVAKTLQSMRHCADFLVRKAELETLRSETQLCEEDLQYVLFLMESCTDFAAKSKQKEVKIKLCKGKPFHKVDITETPPSPPKDDFLKAVDLHYVETRDLSKAPESPTCKPSLEKNLPHFLSHTQDCTCPLCSDLVLSLLCTQWLVAKAENNTQSRGLLKSALKRCKALTQRFSGIVQEILGKGVTKPAVLGVASELMARVYLAMASQSPSKLPEETLEEGLSFVSSRQATLPKHWRAGLLLAKALCSIYNLAAKHGGCVADMFVQLWGWQPHQNGKKAADLNHRNKTTASITKKKTKKMSLDVPSSFPFEDSDTEPPKPHTTPTQKNLPATEARSVTSTLPKKSSITVYNEESPMPEVLPRAPRRRKTRTVLKVDFSDSDLEVPDNPEKEPVSNCRKQAVQTKSAGRGRGRGRSSVAQAPLDDSDDDVTNKSKTRKNRPSTRRGQKESSPTRPVRRRQQVTMDVAPKEVDVLRSIEEEPDWTLDVSFEELRGSDTEQKKTLRTGRKGQKSKNEGLVTECEVLRRDAGGEHLCWTPNMIGGETDLNPFSIAGNSSSLPATFSASDLSLVSSLLHEALESIAHFPPSTLYSRLCRLLALCRGRQDPYVTALLVSESVSITLRHQLLSDIHRKLRKIRREGSGDVCDKFQDLTLEDHKDAQVQHLSQLEEIFQFPKQALDVQRFQEQLHQIPANTTMCILTLVDSQTRMPGDTILLTRLVRGRPPVTVQIPTAHLKVSISSTLQEFDEIQKQQKAVNNLTDKKEWWEGRMELDCRMKALIRLLEEQILGCWKVLLSCPCSSDAVIEECDRLSQTLSDSGWGRTDPELLKILLSCCHFLTPHLIQSFIHGFGFSKPEQAQELVQSAAERLKAGTEQSEGHLVLVLDKHLQKLPWESIPCLQKQSVTRLPSLRFLLSYGLQRKYQPETTLMKGVDPKQAFYVLNPHANLPGTEERFRDWFKNEPGWKGVIRSAPKSEMIQSALTKKDLYIYVGHGAGAHFLDVQTLQRLHCNAVVLLFGCSSVALAVRGDLEGAGIVLKYLMAGCPLVLGNLWDVTDREIDRYTVAFLQSWLKAGSGAPLLKYLSESRQAPKLKYIIGAAPVAYGLPVALR

Target ORF information:

RefSeq Version	XM_031897204.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897204.1

ORF Insert Sequence:

ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT 
CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA 
GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA 
TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG 
TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG 
TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT 
TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA 
CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG 
CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA 
CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA 
GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT 
GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC 
AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA 
TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG 
GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA 
ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT 
TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT 
GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA 
GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC 
AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG 
GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC 
AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC 
TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC 
CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG 
GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT 
CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC 
TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA 
AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG 
GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT 
GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG 
GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT 
TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT 
CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT 
CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA 
AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC 
GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT 
ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC 
AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA 
TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC 
TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT 
CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC 
CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG 
GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG 
GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG 
GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA 
TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT 
GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA 
AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA 
AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG 
AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC 
AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA 
GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG 
TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA 
AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC 
CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA 
AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG 
TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC 
ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA 
GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT 
GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG 
TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA 
CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT 
CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA 
GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC 
CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA 
CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC 
ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC 
CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA 
AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC 
TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA 
CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA 
CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC 
ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG 
ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC 
TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA 
CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA 
GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA 
GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC 
AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT 
CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA 
AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG 
CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA 
GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG 
CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG 
TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG 
GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA 
CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG 
CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG 
GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA 
GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT 
GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA 
GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT 
CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA 
AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG 
CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA 
GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG 
CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT 
CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG 
TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG 
AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA 
CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT 
GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC 
CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA 
GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA 
GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC 
TTACCAGTGG CCCTGCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_031897205.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753065.1 Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

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CloneID	OXa52740
Clone ID Related Accession (Same CDS sequence)	XM_004911948.4 , XM_031897204.1 , XM_031897205.1
Accession Version	XM_031897205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	separin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT 
CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA 
GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA 
TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG 
TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG 
TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT 
TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA 
CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG 
CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA 
CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA 
GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT 
GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC 
AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA 
TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG 
GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA 
ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT 
TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT 
GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA 
GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC 
AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG 
GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC 
AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC 
TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC 
CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG 
GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT 
CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC 
TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA 
AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG 
GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT 
GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG 
GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT 
TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT 
CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT 
CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA 
AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC 
GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT 
ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC 
AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA 
TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC 
TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT 
CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC 
CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG 
GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG 
GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG 
GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA 
TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT 
GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA 
AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA 
AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG 
AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC 
AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA 
GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG 
TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA 
AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC 
CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA 
AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG 
TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC 
ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA 
GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT 
GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG 
TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA 
CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT 
CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA 
GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC 
CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA 
CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC 
ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC 
CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA 
AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC 
TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA 
CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA 
CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC 
ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG 
ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC 
TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA 
CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA 
GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA 
GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC 
AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT 
CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA 
AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG 
CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA 
GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG 
CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG 
TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG 
GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA 
CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG 
CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG 
GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA 
GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT 
GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA 
GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT 
CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA 
AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG 
CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA 
GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG 
CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT 
CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG 
TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG 
AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA 
CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT 
GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC 
CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA 
GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA 
GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC 
TTACCAGTGG CCCTGCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753065.1
CDS	31..6411
Translation	MAGNLKSLKGADFIKLTSSVEEVTLLHSKLKLCLSEVTADQLPDQRVVCDRVLRACNHWAGDPNCHVSHWQSLVPLAALVCQGFCASSPQRTPFYLEKILFHLLKNISARVSSSHACSNLSDLLYGLLTGCPLEQQSAGDFTAVSKSSFQILWKSAGDQEQKQSPSSCNREVLSLRLHALRFLMLAEGSVSHGDAPQTISRHARCAALAFEPKGKPCSKEETYFLSQTLEDLLVKPLLGRGTLSGSWSRCLVELTLERCRRLCKTCCFKQGRHVVEQARGCLEKTERCCHAGLALLTFAVDIHESGIPPSIGEVLSEVIETLDASQSCGDGWMCQALSECFQFAVSSLDGRFKHSGKLKVDDILSLAKFLEKHHQLLEKQVTKVPKDVTRQLKSLKQQQFNSLQQFTNAACAFMQSCKDKAKDGLLLLLGACKDAVSRMHSSLQGLPTQETTEFLNITASCVHNLAYWFYSQKMHQEASDLVYPLNAWLAQSAPDGDPDLAVERLHRCYRLHVESCRKSGQIMKGLEAVGLWLRALRSQVSQHMAEPVALWVRLKMDGAKSGDEDVRLHTLKDGLVDQHVDPELMVSLLAEELQGYKSVHGDTGQERYNTICDLLELCHEDSDLRGHRAIFLLELAQVLCYHDYTGQTDCSALDAVQEALRLLNATSKPTERLQDIKAQALLWQYICTLEANMREGLEEQNRKAKLEGQNQWTGVDYEPNDLNYEDKLHDDQSVRDGICFTLAGETGPLKGLDEALDLWSSLLSSSQVQYLNSAEQTIFSLHLLGSLYRLMGKPLQSMQSFQLAGRLCHSLQDHIKEIGALCHLTKILFYLESPEYAQVILQKAEEILKRADRTSENYTLAELSCQLLRSHLCRVTRQVKEGVELLLGLLQHPSLQKSSKAWYLLKVQVLQELSLFLMLPLENLTSDLYRQLWAHGCHNPETALTEAHKLLRSIVLLLLGNDVLASPKGSSEVRFVDNGENLLQKWQVLADLLSCTHDLVKTLGRMGCVSEAKSFCLEGLRVAKTLQSMRHCADFLVRKAELETLRSETQLCEEDLQYVLFLMESCTDFAAKSKQKEVKIKLCKGKPFHKVDITETPPSPPKDDFLKAVDLHYVETRDLSKAPESPTCKPSLEKNLPHFLSHTQDCTCPLCSDLVLSLLCTQWLVAKAENNTQSRGLLKSALKRCKALTQRFSGIVQEILGKGVTKPAVLGVASELMARVYLAMASQSPSKLPEETLEEGLSFVSSRQATLPKHWRAGLLLAKALCSIYNLAAKHGGCVADMFVQLWGWQPHQNGKKAADLNHRNKTTASITKKKTKKMSLDVPSSFPFEDSDTEPPKPHTTPTQKNLPATEARSVTSTLPKKSSITVYNEESPMPEVLPRAPRRRKTRTVLKVDFSDSDLEVPDNPEKEPVSNCRKQAVQTKSAGRGRGRGRSSVAQAPLDDSDDDVTNKSKTRKNRPSTRRGQKESSPTRPVRRRQQVTMDVAPKEVDVLRSIEEEPDWTLDVSFEELRGSDTEQKKTLRTGRKGQKSKNEGLVTECEVLRRDAGGEHLCWTPNMIGGETDLNPFSIAGNSSSLPATFSASDLSLVSSLLHEALESIAHFPPSTLYSRLCRLLALCRGRQDPYVTALLVSESVSITLRHQLLSDIHRKLRKIRREGSGDVCDKFQDLTLEDHKDAQVQHLSQLEEIFQFPKQALDVQRFQEQLHQIPANTTMCILTLVDSQTRMPGDTILLTRLVRGRPPVTVQIPTAHLKVSISSTLQEFDEIQKQQKAVNNLTDKKEWWEGRMELDCRMKALIRLLEEQILGCWKVLLSCPCSSDAVIEECDRLSQTLSDSGWGRTDPELLKILLSCCHFLTPHLIQSFIHGFGFSKPEQAQELVQSAAERLKAGTEQSEGHLVLVLDKHLQKLPWESIPCLQKQSVTRLPSLRFLLSYGLQRKYQPETTLMKGVDPKQAFYVLNPHANLPGTEERFRDWFKNEPGWKGVIRSAPKSEMIQSALTKKDLYIYVGHGAGAHFLDVQTLQRLHCNAVVLLFGCSSVALAVRGDLEGAGIVLKYLMAGCPLVLGNLWDVTDREIDRYTVAFLQSWLKAGSGAPLLKYLSESRQAPKLKYIIGAAPVAYGLPVALR

Target ORF information:

RefSeq Version	XM_031897205.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897205.1

ORF Insert Sequence:

ATGGCAGGAA ATCTAAAGTC TTTGAAGGGA GCTGACTTTA TAAAACTAAC ATCTTCAGTT 
GAGGAGGTGA CTCTCTTGCA CTCAAAGCTC AAGCTGTGTC TCTCAGAAGT AACTGCCGAT 
CAGTTGCCAG ATCAGCGAGT TGTTTGTGAT CGCGTTCTTC GTGCCTGCAA CCACTGGGCA 
GGTGATCCGA ATTGTCATGT CAGTCACTGG CAGAGCTTAG TGCCCCTGGC AGCTTTGGTA 
TGCCAAGGTT TCTGTGCATC AAGCCCTCAA CGTACCCCCT TCTACCTTGA GAAAATTCTG 
TTTCACCTGC TGAAGAACAT CTCTGCACGT GTATCCTCAA GTCATGCCTG TAGTAATCTG 
TCAGATCTCT TGTATGGCCT TCTAACTGGC TGCCCTTTAG AGCAGCAAAG TGCAGGAGAT 
TTCACAGCTG TGTCAAAGAG CAGTTTTCAG ATCTTGTGGA AGTCTGCAGG GGACCAAGAA 
CAGAAGCAAA GTCCTAGCTC TTGTAACAGA GAAGTGCTGA GCCTTAGATT GCATGCACTG 
CGCTTTTTAA TGTTGGCAGA GGGATCTGTA TCACATGGGG ATGCACCTCA AACTATATCA 
CGCCATGCAA GATGTGCTGC GCTGGCTTTT GAACCCAAGG GTAAACCATG CAGCAAAGAA 
GAGACATATT TTTTAAGTCA GACACTTGAA GATCTCCTTG TGAAACCCCT GTTAGGACGT 
GGTACTTTGT CTGGTTCTTG GTCCCGATGC TTGGTTGAAC TGACACTAGA GAGATGCCGC 
AGACTCTGCA AAACCTGCTG CTTCAAGCAA GGACGACATG TTGTTGAACA AGCACGTGGA 
TGTTTAGAAA AGACAGAGAG ATGCTGCCAT GCTGGGTTGG CACTTTTAAC CTTTGCTGTG 
GACATTCATG AATCTGGCAT ACCGCCTAGC ATTGGTGAAG TTCTGTCTGA AGTGATTGAA 
ACTCTTGATG CTTCCCAATC CTGTGGTGAT GGCTGGATGT GTCAGGCACT CAGTGAATGT 
TTCCAGTTTG CTGTTTCTTC TTTAGATGGG CGTTTCAAGC ATTCTGGGAA GCTGAAGGTT 
GATGACATTT TGAGCCTTGC AAAGTTCCTT GAAAAGCATC ACCAGCTTCT AGAAAAGCAA 
GTGACCAAGG TTCCTAAGGA TGTGACACGA CAACTGAAAT CGTTAAAGCA ACAGCAATTC 
AACAGCCTGC AGCAATTCAC AAATGCTGCC TGTGCCTTTA TGCAGAGCTG CAAGGACAAG 
GCTAAGGATG GATTGCTATT GCTGCTTGGA GCCTGCAAAG ATGCTGTTTC TCGGATGCAC 
AGTTCTCTTC AGGGTCTACC TACTCAAGAA ACCACAGAGT TCTTGAACAT AACAGCATCC 
TGTGTTCATA ACTTGGCCTA CTGGTTCTAT AGCCAGAAGA TGCATCAAGA AGCCTCTGAC 
CTGGTATATC CCCTTAATGC ATGGCTTGCA CAGAGTGCTC CAGATGGGGA CCCTGACCTG 
GCAGTTGAAA GGTTGCACCG CTGCTACAGA CTGCATGTAG AGAGCTGCAG AAAAAGTGGT 
CAGATTATGA AAGGCCTTGA AGCTGTGGGT CTTTGGCTGC GAGCTCTACG CTCTCAAGTC 
TCCCAGCATA TGGCAGAGCC AGTGGCCCTG TGGGTTAGAC TTAAAATGGA TGGTGCTAAA 
AGTGGAGATG AAGACGTAAG GCTGCATACT CTAAAAGATG GCCTTGTTGA TCAGCACGTG 
GACCCGGAGC TGATGGTATC ACTTCTTGCG GAGGAGCTTC AAGGATACAA ATCAGTCCAT 
GGCGATACAG GGCAGGAACG ATACAATACA ATATGTGATC TCCTTGAGCT CTGCCATGAG 
GACAGCGACC TTAGAGGCCA TCGTGCAATA TTTCTTCTAG AGCTGGCCCA AGTCTTGTGT 
TACCATGACT ACACTGGACA GACAGACTGC TCTGCCCTGG ATGCTGTTCA GGAAGCTCTT 
CGACTTTTAA ATGCAACTTC AAAACCCACA GAACGTTTGC AGGACATTAA AGCCCAAGCT 
CTGCTCTGGC AGTATATCTG TACCCTTGAA GCAAACATGC GGGAGGGTTT GGAGGAGCAA 
AACCGTAAGG CAAAGTTAGA GGGTCAGAAC CAGTGGACAG GTGTGGATTA TGAGCCCAAC 
GACCTGAACT ATGAAGATAA ACTACATGAT GACCAGTCTG TCAGGGATGG GATCTGCTTT 
ACATTGGCAG GGGAGACAGG GCCACTGAAG GGGCTAGATG AAGCTCTTGA TCTGTGGAGC 
AGTCTCTTAT CCAGCTCACA AGTACAATAC TTAAACAGTG CTGAACAAAC CATTTTTTCA 
TTGCACCTGC TCGGATCATT GTACAGACTG ATGGGAAAAC CTCTGCAATC CATGCAGAGC 
TTTCAGCTTG CTGGACGCCT GTGCCACTCT CTACAAGATC ATATTAAGGA GATTGGGGCT 
CTCTGCCATC TCACCAAAAT TTTATTCTAC TTGGAGAGTC CTGAATATGC TCAAGTTATC 
CTTCAGAAGG CAGAGGAGAT CTTAAAACGC GCTGATCGTA CTAGTGAAAA TTACACTCTG 
GCTGAGCTAT CCTGTCAGCT TTTGCGTAGT CATCTCTGCA GGGTCACACG TCAGGTTAAG 
GAAGGGGTAG AACTGCTTCT GGGTCTTCTT CAGCACCCTT CTCTGCAGAA ATCATCAAAG 
GCATGGTACC TTCTCAAAGT ACAAGTTCTT CAAGAGCTTT CATTGTTTCT CATGCTCCCA 
TTAGAAAACT TAACCTCTGA TCTATACAGG CAGTTATGGG CTCATGGGTG TCATAATCCT 
GAGACTGCTC TGACAGAAGC CCATAAGCTG CTAAGGAGCA TCGTCCTCCT CCTTCTTGGA 
AATGATGTTT TGGCTTCCCC TAAAGGTTCT TCAGAAGTGC GTTTTGTGGA TAATGGAGAA 
AACCTTCTTC AAAAATGGCA AGTCCTTGCT GATCTCCTCA GCTGCACCCA TGATCTAGTG 
AAGACTCTTG GCCGTATGGG CTGTGTTTCA GAAGCCAAGT CATTTTGCCT TGAGGGACTC 
AGAGTGGCCA AAACTCTGCA GAGTATGCGG CACTGCGCTG ATTTCCTTGT GAGAAAAGCA 
GAACTAGAGA CTCTGCGCTC AGAAACTCAG CTCTGTGAAG AAGATTTGCA GTATGTGCTG 
TTCCTAATGG AGTCCTGTAC AGATTTTGCA GCCAAAAGTA AACAGAAGGA GGTCAAAATA 
AAGCTTTGCA AAGGGAAACC ATTTCATAAG GTGGACATTA CAGAAACACC TCCAAGTCCC 
CCTAAAGATG ATTTTCTGAA GGCAGTAGAT CTTCATTATG TAGAAACAAG GGATTTGTCA 
AAAGCCCCTG AATCTCCAAC ATGCAAGCCT TCCCTGGAAA AGAACCTTCC ACATTTTTTG 
TCTCATACAC AGGACTGCAC TTGCCCTCTA TGCTCTGATC TTGTACTGTC CTTGCTTTGC 
ACCCAGTGGT TGGTGGCTAA GGCAGAGAAT AATACTCAGA GCAGAGGTTT GTTAAAATCA 
GCCCTGAAAA GATGCAAGGC ACTAACCCAG CGCTTTTCTG GTATTGTTCA GGAAATACTT 
GGTAAGGGAG TCACCAAACC AGCAGTTCTA GGAGTTGCAT CTGAACTTAT GGCTAGAGTG 
TACCTGGCCA TGGCATCACA GAGCCCCTCT AAGCTTCCAG AAGAGACCCT GGAGGAAGGA 
CTTAGTTTTG TGTCTTCACG CCAAGCAACT CTTCCTAAGC ACTGGAGAGC GGGCCTTCTT 
CTGGCCAAAG CTTTGTGCTC TATATATAAT CTGGCTGCTA AGCATGGTGG CTGTGTTGCA 
GATATGTTTG TTCAATTATG GGGCTGGCAG CCACATCAGA ACGGCAAAAA AGCAGCAGAC 
CTCAATCACA GGAACAAGAC AACCGCTAGC ATTACAAAGA AAAAAACTAA GAAAATGTCA 
CTGGATGTCC CAAGTTCATT CCCCTTTGAG GACTCGGACA CTGAGCCTCC AAAACCTCAC 
ACAACTCCCA CCCAGAAGAA CCTTCCAGCA ACTGAAGCAA GATCTGTTAC TTCTACCCTC 
CCAAAGAAAA GCTCTATTAC AGTTTATAAT GAAGAATCCC CCATGCCTGA AGTATTACCA 
AGAGCACCAA GAAGGCGCAA AACAAGAACA GTGCTAAAGG TTGATTTTAG TGACAGTGAC 
TTGGAAGTGC CAGATAATCC TGAAAAGGAG CCAGTGTCAA ACTGCAGGAA ACAAGCTGTA 
CAGACTAAAT CAGCAGGTCG AGGTCGAGGT CGGGGGAGGA GCAGTGTTGC ACAGGCCCCA 
CTTGATGACT CTGATGATGA TGTTACAAAT AAGAGTAAGA CCAGGAAAAA TAGACCCAGC 
ACAAGGAGGG GACAGAAAGA AAGTAGCCCA ACTAGGCCAG TGCGGAGGCG CCAACAAGTG 
ACAATGGATG TGGCTCCTAA AGAAGTAGAT GTCTTAAGAT CTATAGAGGA AGAACCTGAC 
TGGACGCTGG ATGTCAGCTT CGAAGAGCTG AGGGGTTCTG ATACAGAGCA AAAGAAAACA 
CTTAGAACTG GTCGGAAGGG TCAGAAGTCT AAGAATGAAG GGTTGGTGAC AGAATGTGAA 
GTGCTAAGAA GAGATGCTGG AGGGGAACAC TTGTGTTGGA CCCCGAATAT GATAGGAGGA 
GAAACTGACC TCAACCCATT TAGCATCGCA GGAAATTCAT CTTCTCTACC TGCTACTTTC 
AGTGCCTCTG ACCTGAGTTT AGTCTCCTCC CTTCTGCATG AGGCTCTGGA GTCAATAGCT 
CACTTTCCAC CAAGCACCCT CTATTCCAGG CTTTGCCGAC TTCTTGCCCT GTGCAGAGGA 
AGACAAGATC CATATGTCAC GGCTCTTCTA GTTAGCGAGT CTGTTTCCAT TACATTGAGG 
CACCAATTGC TGAGCGACAT TCACCGGAAA TTACGGAAAA TACGGAGAGA AGGCTCAGGA 
GATGTGTGTG ACAAGTTTCA GGATCTAACT CTGGAGGATC ATAAAGATGC TCAGGTTCAG 
CACCTCTCCC AGCTAGAGGA GATCTTCCAA TTCCCAAAAC AAGCTCTGGA CGTACAAAGG 
TTCCAAGAGC AACTACACCA GATCCCCGCA AATACAACAA TGTGTATATT GACCTTGGTG 
GACTCGCAGA CAAGAATGCC TGGTGATACT ATACTGCTTA CTAGACTGGT TCGAGGAAGA 
CCCCCTGTCA CAGTCCAGAT TCCTACTGCT CATCTGAAGG TGTCCATAAG CTCTACGTTG 
CAAGAATTTG ATGAAATCCA AAAGCAGCAG AAGGCTGTCA ACAACCTGAC AGACAAAAAG 
GAATGGTGGG AAGGGCGCAT GGAGTTGGAT TGTCGAATGA AGGCTTTAAT ACGTTTACTA 
GAGGAACAGA TTTTGGGCTG CTGGAAAGTG TTGCTAAGCT GCCCATGCTC CAGTGATGCT 
GTCATAGAGG AATGTGACCG GCTTTCACAG ACACTGTCAG ATTCTGGCTG GGGAAGAACA 
GATCCTGAAC TCCTGAAGAT TTTGCTGAGT TGTTGCCATT TCCTAACCCC TCACCTTATT 
CAGTCCTTCA TCCACGGATT TGGATTTTCA AAGCCTGAGC AGGCTCAGGA GCTGGTACAA 
AGTGCCGCAG AGAGACTTAA AGCGGGCACA GAACAATCAG AAGGACATCT GGTGCTTGTG 
CTGGATAAGC ATCTCCAGAA GCTTCCGTGG GAGAGCATCC CCTGCCTGCA GAAGCAGTCA 
GTCACAAGGC TTCCATCTCT ACGCTTCCTT CTCAGCTATG GCTTGCAAAG GAAGTACCAG 
CCTGAGACAA CTCTAATGAA AGGAGTTGAC CCAAAGCAAG CCTTTTATGT CCTTAACCCT 
CATGCAAACC TGCCAGGCAC CGAGGAGCGC TTCAGGGACT GGTTCAAAAA TGAACCTGGG 
TGGAAGGGAG TAATCAGATC GGCTCCAAAA TCTGAGATGA TTCAGTCTGC ACTGACAAAG 
AAAGATCTCT ACATCTATGT TGGGCACGGT GCAGGGGCAC ACTTTTTGGA TGTACAGACA 
CTCCAAAGGT TACACTGCAA TGCTGTGGTT TTGCTTTTTG GGTGCAGCAG TGTTGCCCTT 
GCAGTTCGGG GAGATCTGGA AGGTGCAGGA ATTGTTTTAA AATACCTTAT GGCCGGATGC 
CCTTTAGTTC TTGGCAACCT ATGGGACGTC ACTGACCGTG AGATTGACCG CTATACCGTA 
GCATTTTTAC AAAGTTGGCT CAAGGCAGGA TCTGGAGCTC CTCTCTTGAA ATACCTGTCA 
GAATCCCGCC AAGCTCCCAA GCTGAAATAC ATCATTGGTG CAGCTCCTGT AGCTTATGGC 
TTACCAGTGG CCCTGCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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espl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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