ESPL1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESPL1
Entrez Gene ID	506740
Full Name	extra spindle pole bodies like 1, separase
General protein information	Preferred Names extra spindle pole bodies like 1, separase Names separin extra spindle pole bodies homolog 1 extra spindle poles like 1 extra spindle poles-like protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045949.2	NP_001039414.2	separin

Pathways from BioSystems

Homologies

Homo sapiens (human)	ESPL1	NP_036423.4
Macaca mulatta (Rhesus monkey)	ESPL1	XP_001103494.1
Danio rerio (zebrafish)	espl1	XP_001337869.1
Pan troglodytes (chimpanzee)	ESPL1	XP_003313754.1
Canis lupus familiaris (dog)	ESPL1	XP_849828.2
Bos taurus (cattle)	ESPL1	NP_001039414.2
Gallus gallus (chicken)	ESPL1	XP_423289.4
Mus musculus (house mouse)	Espl1	NP_001014976.1
Rattus norvegicus (Norway rat)	Espl1	NP_001164073.1
Xenopus tropicalis (tropical clawed frog)	espl1	XP_004912005.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ESPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa40711	NM_001045949.2 Latest version!	Bos taurus extra spindle pole bodies like 1, separase (ESPL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESPL1 ( NM_001045949.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001039414.2 Bos taurus extra spindle pole bodies like 1, separase (ESPL1), mRNA.

Price & Availability↑

CloneID	OBa40711
Clone ID Related Accession (Same CDS sequence)	NM_001045949.2
Accession Version	NM_001045949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527091200000
Organism	Bos taurus(cattle)
Product	separin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On Dec 12, 2009 this sequence version replaced NM_001045949.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571259.40246.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCTCTA GGACGCCCTC CGGCACCATG AGGAGCTTCA GAGGAGTCAA CTTTGGGACC 
CTGCTAAGCA GTCCAAAGGA GACTGAAGAG CTGCTGCCTG ACTTGAAGGA GTTCCTGACC 
AAGCTTCCCA GCTGCCGGTC TGATTCCGAG AGGAGACAAA CTTGTGATGC CATTCTGAGG 
GCTTGTAACC AGCAGCTGGC TGTCAAGCTG GCTTGCCCTA GGCACCTGGG AAGCCTGCTG 
GAGCTGGCGG AGCTGGCTTG CGAGGGCTAC TTGATGTCTA CACCCCAGCG CCCACCCCTC 
TACCTGGAAC GAATTCTCTT CATCTTTCTG CGGAATGTTG CTGCACAGGG AGACCCAGAA 
GCCACTCTCC GACTCGCTCA GCCCCTCCAT GCCTGCTTGG TGCAATGTCC TCGCCAAGCT 
GCTCCTCAGG ACTACGAGGC TGTGGCTCGG GGCAGCTTTT CTCTGCTTTG GAAGGGGGCA 
GAAGGCCTGA TGGAGCGGCG AGCTGCATTC TCAGCTCGGT TAAAGGCCTT GAGCTTTCTA 
GTACTCTTGG AGGATGAAAG TATCCCTTGT GAGATTCCCC ACTTTGCTTC TCCAACAGCC 
TGTCGAGTGG TAGCTGCCCA TCAGCTATTT GAAGCCAGTG GGCATGGTCT AAATGAAGCA 
GATGCTGATT TCCTAGATGA CCTGCTCTCC AGGCATGTGA TCACAGCTTT GGTGGGTGAA 
GGAGCCTCTC CTGGTCCTCT TTCCCCACAG AGGGCCCTCT GTCTCTTGGA GCTCACCCTG 
GAACACTGTC GTCGCCTCTG CTGGAACCAC CACCACACCA GGGCCACAAG TACAGTGGAG 
AAGGCCCACG ATTACCTAAG GAACACCAGT CTGGCCCCCA GCCTCCAGCT GTGCCAGCTG 
GCAGTTGAAC AGTTGCTGGC TGGGGAAGGA GGACCCCAGG CAGTGGCCAA GCTTCTGATC 
AAGGCAACAG CTGTCCTGAA GAACAGCTTG GAGGCACCAT CACCCCCACT ACGGGCATTG 
TGTGACAGCT GCCAGTTCTT CCTCTCAGTC CTGGAGCGAG GCACCAAGAG GTGCTATGGA 
CTTGATGCCA TTCTGAGCCT CTTTGCTTTT CTTGGCAGCC ACTGTTCCCT CATCCAGCAG 
CTGCAGGATG GTGTCTGTGG GGACTCCTCC AAGCAGCTGC AGGCTTTGGT TCAGATACAC 
TTTCAGGGAC TTCACCTCTA CACTGCGGTG GTTTATGACT TTGCCCAAGG CTGTCAGGAA 
GCTAATTTGG CTGACCTGGC CCGGCTAGTG GACAGTTGCA GATCTACTGT TACCTGGATG 
CTGGAGGCCT TACAGGGCCT GTCTGGCCGA GAGCTGACTG ACTACCTGGG GATGACTGTC 
TCTTACACCA GTAACCTGGC CTACAGCTTC TACAGTCACA AGCTCTATGC CGAAGCCTGT 
GCCATCTCCG AGCCCCTCTG TCAGCACCTG GGCTCAGTGA AGCCAGGCAC CTATCCTGAG 
GTGCCCCCTG AGAAGTTGCA CAGGTGCTTC CGGCTACACA TAGAGAGTTT AAAGAAACTG 
GGTAAACAGG CCCAGGGCTG CAAAATGGTG ACTTTGTGGC TGGCAGCCCT GCAGCCCTGT 
GGCCCCGAAC ACATGACTGA GCCAGTCACC TTCTGGGTTC GGGTCAAGAT GGATGCTGCC 
AGAGCCGGAG ACAAGGAGCT ACAGCTGAAG ACGCTGCGAG ACAGCCTGAG TGGCTGGGAC 
CCGGAGAGCC TGGCCCGCTT GCTCAGGGAG GAGCTGCAGG CCTACAAGGC AGTGCGGGCC 
AACACAGGGC AGGAACGGTT CAATGTCATC TGTGACCTGC TGGAGCTGAG CCCCGAGGAG 
ACGCCAGCTG GGGCCTGGGC CCGAGCCACC CACCTGGTGG AACTGGCTCA GGTGCTCTGC 
TATCACAACT TTGCCCAGCA GACTGACTGC ACTGCCCTCG ATGCCATCCG GGAAGCTCTG 
CAGCTTCTGG AGTCTGTGAA GCCTGAGGCC CAGGACAAAG ATCGGCTTTT GGATGATAAA 
GCCCAGGCCC TGCTGTGGCT CTACATCTGT ACCCTGGAGG CCAAAATGCA GGAGGGTATT 
GAGCGGGATC GGAGAGCCCA GGCCCCTTGT AACCTGGAGG AGTTTGAAGT CAATGACCTG 
AATTATGAAG ATAAACTCCA GGAAGATCGC TTCCTATACA GTAACATTGC CTGCAACCTG 
GCTGCGGATG CTGCACAGTC CAAATGCCTG GACCAAGCCC TGGCCCTGTG GAAGGAGGTG 
CTTACTAAGG GGCAGGTGCC CGCTGTACGG TGTCTCCAGC AAACAGCAGC CTCCCTGCAG 
ATCCTAGCGG CCCTCTATCA GCTGGTGGCA AAGCCCCTGC AGGCTCTGGA GGTCCTCCTG 
CTCGTCCAGA TGGTCTCACA GAGACTGAAG GACCATGCGA AGGCAGCCGG CTCTTCCTGC 
CAAGTCACCC AGCTCCTCCT GACCCTCGGC TGTCCCAGCT ACGCCCAGGT GCACCTGGAA 
GAAGCAGAGT CGAGTCTGAA GCTTCTGGAT CGCACCACAG ACACATACCT GCTCCTCTCC 
CTGACCTGTG ACCTCCTACG AAGTCGACTC TGCTGCGCTC GCCAGAAGGT GGCTGAGGGT 
GCTGCTCTGC TGCTGTCTGT GCTTCAGGAC CCTGCCCTCC AGAGGGCATC CAAGGCCTGG 
TACTTGCTGC GTGTCCAGGC CCTGCAGCTG GTGGCAGCCT ACCTGAGTCT CTCGCCAGAC 
AGCCTGCTGG CCCCGCTGTG GGAGCAACTG TGTGCCCAAG GCTGGCAGAC ACCTGAGATA 
GCACTCATCG ACTCCCATAA GCTTCTCCGA AGCATCATCC TCCTGCTCAT GGGCAGCGAT 
GTGCTCTCAA TTCAGAAAAC AGCTGTGGAG ACGCCATTTC TGGACTACGG TGAAAATCTG 
GTACAAAAAT GGCAGGTTCT TTCTGAGGTG CTGAGCTGCT CGGAGAAGCT GGTCTCCCGC 
CTGGGCCGCC TTGGTAGTGT GAGCGAAGCC AAAGCCTTTT GCTTGGAGGC CCTGAAACTT 
ACAACAAAGC TGCAGATACC ACACCAGTGT GCCCTCTTCC TGGTGCTGAA GGGGGAGCTG 
GAGCTGGCAC GCAATGACAT GGACCTCTGT CAGTCGGACC TGCATCAAGT TCTGTTCTTG 
CTTGAGTCTT GCACAGAGTT TCTCGGACTA GCGCAGCACC CGGACTCTGT GAAGAAGGTC 
CACCTGCAGA AGAGGAAGCA GCAGGCTCGG GTGCCCCATG CTCCAGAGCT CCCGGACGAA 
GAGCTCATTC TGAAAGGCCC TGCCCTGGAG CTGGTGGCCA CCGTGGCCAA GGAACCTGGC 
CCTGTAGCAC CTTCTGCTAA CTCCTCCCCG ATCCTGAAAA CCAAGCCCCA GCCCAGCCCC 
AGCTTCCTGA CCCACTTGCC CACCTGTGAC TGCTCGCTCT GTGCCAGCCC TGTCCTCTCG 
GTGGTCTGTC TACGCTGGGC GTTGGTCACA GCGGGGCTGA GACTGGCCAT GGGCCATGAG 
GCCCAGGGGC TGGATCTGCT GCAGGTGGTG CTGAAGGGTT GCCCTGCAGC CAGTGGTCGC 
CTCGCCCAAG CTCTGCAGGC TTCCCTGAAT CACACAGCGC CCCCCTCCCC TGTCCCAAGC 
CTCTTTGATG AGATCTTGGC TCAGGCATAT ACACAGCTGG CACTGGAGGG GCTGAGCCAG 
CCATCAAACA AGAGCCTGGG AAAGGTCCTG GAGTCAGGGT TAAAGTTTGT GGTGGCGCGG 
ATACCCCACC TGGAGCCCTG GCGAGCCAGC CTGCTCTTGG TCCGGGCCCT TGCAAAGCTG 
ACCAGCCTCA GCTGCTGCAC TGCCCAACTT TTTGCAAGCT CCTGGGGCTG GCAGCCGCCA 
TCAATAAAGA ACCCCACAGG CTCAGAACCC TCTAAGCCTC GGAACCAAAA ACAGTCAGGA 
CAGTCCAGAC GAGGACGCCA GCAAGTGGGC TCTGCTACCC TGTCCCTCTC ACATACCTCT 
CTGAAAGGTC TGGAAGGTGA AGGACCACCC TGTACACCTA AGCCCCCAGG CCGGGTCAGG 
CAGGCCGGCC CTGGGGTACC CTTCACCGTA TTTGAGGAAG TCTTCCTTAC AAAGACCAAG 
CCTGAGGTTC CCAAGGCCCC CAGGGTACAA CGGAGGATCC AGACACGCCT CAAGGTGAAC 
TTCAGTGATG ACAGTGACTT GGAAGACCTT GTCTCAGTTG AAGCACAGCT TGCAGAGGGG 
TCCAAGAGAC AGGGCACTAC TTCCCGGGGC CGGGGCCGGG CCAGGAAGGG CCCGAGTGTG 
AAGACTAACT CAGTGGCCAC GTCAGGCAGT GCCCCCGGCC TCAGTGGCAG AAGCCGGAGG 
GTCAAGAAGG TGGCATCAGG ACATTGTGAG GAGCCGGGCC CTGAGATGAT GAGGACCATC 
CCTGAGGAAC TGACTGACAA CCAGATGGAA ATGAGCTTTG AGATCCTCAG GGGCTCTGAT 
GGGGAAGACT CAGCCTCAGG TGGGAAGACA CCAGGGCCTG ATTCAGCCGT AGGAGAATGT 
GAGGTGTTGA GACGGGATGC CAGCAAAGAG GAGCTGCCTG GCCCTGGCCC AGACAAGGAG 
AGAGACATGG ATCTGGGTCC CCGGCACAGA CTCCCCTCGG CCCCTGTAGC CATCGATCTG 
TCTACCCTGG ATTCCATCTG TGACTCACTA AGTGGTGCTT TCCGTGGCAT CAGTCACTGC 
CCTCCTAGTG GGCTCTATGC CCACCTCTGC CGCCTCCTGG CCTTGTGCCT GGGCCACCGG 
GATCCCTATG CCACTGCCTG CCTTGTCACC GAGTCTGTCT CCGTCACCTG TCGCCACCAG 
CTGCTTACCC ACCTCCACAG GCAGCTCAGC AAGGCCCAGA AGCACCGAGG GTCGCCAGAC 
ATAGCAGACC AGTTGCAGGG GCTGCACCTC CAGGAGAAGC CTGGAGACAT CCCTCTGGCA 
CGAATCCAGC GCCTCTTTGC CTTCAGGCCT TTGGGATCTG GCCACTTCCC CCAGCCTGAG 
AAGGACAGCT TCCAGGAGCG TCTTGCTCTG ATTCCCAGTG GGGTGACCGT GTGTGTGTTG 
GCCCTGGCCA CCCTCCAGCC TGGAACTGTG GGCAACACCC TCCTGCTGAC CCGGCTGGAA 
AAAGACAAAC CCCCGGTCAC TGTACAGATC CCCACTTCCC AGAGCAAGCT TTCTGTGAGT 
TCGGCCTTGA AAGAGTTTGA CGCCATCCAG AAGGAGCAGA AAGAGAACAG CAGCTGTACT 
GACAAGCGAG AATGGTGGAC TGGCCGGCTA GAGCTGGATC GCAGGATGGA GGTTCTCACC 
ACTTCCCTAG AGAAGTACGT GCTGGGCTGC TGGCGGGGGC TGTTGCTGCC ATCCTGCGAG 
GACCCCGACC TTGTCCGGGA GGCCTCCCAT CTACAAGACT CACTACAGGA ATGTGGCTGG 
AAATATCCTG ACCCCACTCT GCTGAGAATC ATGCTCAGCG GTGCCAGTAC CCTCACGCCG 
CAGGACGTGC AGGCCCTGGC TTATGGGCTG TGCCCAGGCC GACCAGCCCG AGCCCAAGAG 
CTCCTAGGTG AGGCAGTAGG GCGTCTCCAA GGTCAGACAT CAACAAGCAG TAGGCATCTC 
GTGTTAGTGC TGGACAAGGA CCTACAGAAG CTGCCGTGGG AGAGCATACC CAGCCTCCGG 
GCCCTGCCTG TCACTCGACT GCCCTCCTTC CGATTCCTAC TCAGCTACTC CATCATCAAA 
GAGTCTGGGG CCTCATCAGT GCTGAGCCAA GGAGTGGATC CTCGCAGAAC CTTCTACGTC 
CTGAACCCTC ACAATAACCT GTCAAGCACA GAGGAGCAAT TCCGAGCCCA TTTTAGCAGT 
GAAGCTGGCT GGAAAGGGGT GGTTGGGGAG GTGCCGAGCC CGGAACAGGT GCAAACGGCC 
TTGACGGAGC GTGACTTGTA CATCTATGCA GGGCATGGCG CCGGCGCCCG CTTCCTGGAC 
GGGCAGGCTG TCCTGCGGCT GAGCTGCCGG GCAGTGGCCC TGCTGTTCGG CTGCAGCAGC 
GCAGCCCTGG CTGTGCATGG AAACCTGGAG GGGGCTGGCA TTGTGCTCAA GTATATCATG 
GCTGGCTGCC CCCTGTTCCT AGGTAACCTC TGGGACGTGA CTGACCGTGA CATTGACCGC 
TACACAGAGG CTCTGTTGCA GGGCTGGCTT AGAGCAGGTT CAGGAGCCCC CCTTCTGTAC 
TATGTCAGCC AGGCCCGCCA GGCTCCCCGA CTCAAATACC TTATTGGGGC TGCACCTGTA 
GCCTATGGCT TGCCTGTCTC CCTGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039414.2
CDS	77..6406
Translation	MISRTPSGTMRSFRGVNFGTLLSSPKETEELLPDLKEFLTKLPSCRSDSERRQTCDAILRACNQQLAVKLACPRHLGSLLELAELACEGYLMSTPQRPPLYLERILFIFLRNVAAQGDPEATLRLAQPLHACLVQCPRQAAPQDYEAVARGSFSLLWKGAEGLMERRAAFSARLKALSFLVLLEDESIPCEIPHFASPTACRVVAAHQLFEASGHGLNEADADFLDDLLSRHVITALVGEGASPGPLSPQRALCLLELTLEHCRRLCWNHHHTRATSTVEKAHDYLRNTSLAPSLQLCQLAVEQLLAGEGGPQAVAKLLIKATAVLKNSLEAPSPPLRALCDSCQFFLSVLERGTKRCYGLDAILSLFAFLGSHCSLIQQLQDGVCGDSSKQLQALVQIHFQGLHLYTAVVYDFAQGCQEANLADLARLVDSCRSTVTWMLEALQGLSGRELTDYLGMTVSYTSNLAYSFYSHKLYAEACAISEPLCQHLGSVKPGTYPEVPPEKLHRCFRLHIESLKKLGKQAQGCKMVTLWLAALQPCGPEHMTEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPESLARLLREELQAYKAVRANTGQERFNVICDLLELSPEETPAGAWARATHLVELAQVLCYHNFAQQTDCTALDAIREALQLLESVKPEAQDKDRLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPCNLEEFEVNDLNYEDKLQEDRFLYSNIACNLAADAAQSKCLDQALALWKEVLTKGQVPAVRCLQQTAASLQILAALYQLVAKPLQALEVLLLVQMVSQRLKDHAKAAGSSCQVTQLLLTLGCPSYAQVHLEEAESSLKLLDRTTDTYLLLSLTCDLLRSRLCCARQKVAEGAALLLSVLQDPALQRASKAWYLLRVQALQLVAAYLSLSPDSLLAPLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDVLSIQKTAVETPFLDYGENLVQKWQVLSEVLSCSEKLVSRLGRLGSVSEAKAFCLEALKLTTKLQIPHQCALFLVLKGELELARNDMDLCQSDLHQVLFLLESCTEFLGLAQHPDSVKKVHLQKRKQQARVPHAPELPDEELILKGPALELVATVAKEPGPVAPSANSSPILKTKPQPSPSFLTHLPTCDCSLCASPVLSVVCLRWALVTAGLRLAMGHEAQGLDLLQVVLKGCPAASGRLAQALQASLNHTAPPSPVPSLFDEILAQAYTQLALEGLSQPSNKSLGKVLESGLKFVVARIPHLEPWRASLLLVRALAKLTSLSCCTAQLFASSWGWQPPSIKNPTGSEPSKPRNQKQSGQSRRGRQQVGSATLSLSHTSLKGLEGEGPPCTPKPPGRVRQAGPGVPFTVFEEVFLTKTKPEVPKAPRVQRRIQTRLKVNFSDDSDLEDLVSVEAQLAEGSKRQGTTSRGRGRARKGPSVKTNSVATSGSAPGLSGRSRRVKKVASGHCEEPGPEMMRTIPEELTDNQMEMSFEILRGSDGEDSASGGKTPGPDSAVGECEVLRRDASKEELPGPGPDKERDMDLGPRHRLPSAPVAIDLSTLDSICDSLSGAFRGISHCPPSGLYAHLCRLLALCLGHRDPYATACLVTESVSVTCRHQLLTHLHRQLSKAQKHRGSPDIADQLQGLHLQEKPGDIPLARIQRLFAFRPLGSGHFPQPEKDSFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDKPPVTVQIPTSQSKLSVSSALKEFDAIQKEQKENSSCTDKREWWTGRLELDRRMEVLTTSLEKYVLGCWRGLLLPSCEDPDLVREASHLQDSLQECGWKYPDPTLLRIMLSGASTLTPQDVQALAYGLCPGRPARAQELLGEAVGRLQGQTSTSSRHLVLVLDKDLQKLPWESIPSLRALPVTRLPSFRFLLSYSIIKESGASSVLSQGVDPRRTFYVLNPHNNLSSTEEQFRAHFSSEAGWKGVVGEVPSPEQVQTALTERDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLRAGSGAPLLYYVSQARQAPRLKYLIGAAPVAYGLPVSLR

Target ORF information:

RefSeq Version	NM_001045949.2
Organism	Bos taurus(cattle)
Definition	Bos taurus extra spindle pole bodies like 1, separase (ESPL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045949.2

ORF Insert Sequence:

ATGATCTCTA GGACGCCCTC CGGCACCATG AGGAGCTTCA GAGGAGTCAA CTTTGGGACC 
CTGCTAAGCA GTCCAAAGGA GACTGAAGAG CTGCTGCCTG ACTTGAAGGA GTTCCTGACC 
AAGCTTCCCA GCTGCCGGTC TGATTCCGAG AGGAGACAAA CTTGTGATGC CATTCTGAGG 
GCTTGTAACC AGCAGCTGGC TGTCAAGCTG GCTTGCCCTA GGCACCTGGG AAGCCTGCTG 
GAGCTGGCGG AGCTGGCTTG CGAGGGCTAC TTGATGTCTA CACCCCAGCG CCCACCCCTC 
TACCTGGAAC GAATTCTCTT CATCTTTCTG CGGAATGTTG CTGCACAGGG AGACCCAGAA 
GCCACTCTCC GACTCGCTCA GCCCCTCCAT GCCTGCTTGG TGCAATGTCC TCGCCAAGCT 
GCTCCTCAGG ACTACGAGGC TGTGGCTCGG GGCAGCTTTT CTCTGCTTTG GAAGGGGGCA 
GAAGGCCTGA TGGAGCGGCG AGCTGCATTC TCAGCTCGGT TAAAGGCCTT GAGCTTTCTA 
GTACTCTTGG AGGATGAAAG TATCCCTTGT GAGATTCCCC ACTTTGCTTC TCCAACAGCC 
TGTCGAGTGG TAGCTGCCCA TCAGCTATTT GAAGCCAGTG GGCATGGTCT AAATGAAGCA 
GATGCTGATT TCCTAGATGA CCTGCTCTCC AGGCATGTGA TCACAGCTTT GGTGGGTGAA 
GGAGCCTCTC CTGGTCCTCT TTCCCCACAG AGGGCCCTCT GTCTCTTGGA GCTCACCCTG 
GAACACTGTC GTCGCCTCTG CTGGAACCAC CACCACACCA GGGCCACAAG TACAGTGGAG 
AAGGCCCACG ATTACCTAAG GAACACCAGT CTGGCCCCCA GCCTCCAGCT GTGCCAGCTG 
GCAGTTGAAC AGTTGCTGGC TGGGGAAGGA GGACCCCAGG CAGTGGCCAA GCTTCTGATC 
AAGGCAACAG CTGTCCTGAA GAACAGCTTG GAGGCACCAT CACCCCCACT ACGGGCATTG 
TGTGACAGCT GCCAGTTCTT CCTCTCAGTC CTGGAGCGAG GCACCAAGAG GTGCTATGGA 
CTTGATGCCA TTCTGAGCCT CTTTGCTTTT CTTGGCAGCC ACTGTTCCCT CATCCAGCAG 
CTGCAGGATG GTGTCTGTGG GGACTCCTCC AAGCAGCTGC AGGCTTTGGT TCAGATACAC 
TTTCAGGGAC TTCACCTCTA CACTGCGGTG GTTTATGACT TTGCCCAAGG CTGTCAGGAA 
GCTAATTTGG CTGACCTGGC CCGGCTAGTG GACAGTTGCA GATCTACTGT TACCTGGATG 
CTGGAGGCCT TACAGGGCCT GTCTGGCCGA GAGCTGACTG ACTACCTGGG GATGACTGTC 
TCTTACACCA GTAACCTGGC CTACAGCTTC TACAGTCACA AGCTCTATGC CGAAGCCTGT 
GCCATCTCCG AGCCCCTCTG TCAGCACCTG GGCTCAGTGA AGCCAGGCAC CTATCCTGAG 
GTGCCCCCTG AGAAGTTGCA CAGGTGCTTC CGGCTACACA TAGAGAGTTT AAAGAAACTG 
GGTAAACAGG CCCAGGGCTG CAAAATGGTG ACTTTGTGGC TGGCAGCCCT GCAGCCCTGT 
GGCCCCGAAC ACATGACTGA GCCAGTCACC TTCTGGGTTC GGGTCAAGAT GGATGCTGCC 
AGAGCCGGAG ACAAGGAGCT ACAGCTGAAG ACGCTGCGAG ACAGCCTGAG TGGCTGGGAC 
CCGGAGAGCC TGGCCCGCTT GCTCAGGGAG GAGCTGCAGG CCTACAAGGC AGTGCGGGCC 
AACACAGGGC AGGAACGGTT CAATGTCATC TGTGACCTGC TGGAGCTGAG CCCCGAGGAG 
ACGCCAGCTG GGGCCTGGGC CCGAGCCACC CACCTGGTGG AACTGGCTCA GGTGCTCTGC 
TATCACAACT TTGCCCAGCA GACTGACTGC ACTGCCCTCG ATGCCATCCG GGAAGCTCTG 
CAGCTTCTGG AGTCTGTGAA GCCTGAGGCC CAGGACAAAG ATCGGCTTTT GGATGATAAA 
GCCCAGGCCC TGCTGTGGCT CTACATCTGT ACCCTGGAGG CCAAAATGCA GGAGGGTATT 
GAGCGGGATC GGAGAGCCCA GGCCCCTTGT AACCTGGAGG AGTTTGAAGT CAATGACCTG 
AATTATGAAG ATAAACTCCA GGAAGATCGC TTCCTATACA GTAACATTGC CTGCAACCTG 
GCTGCGGATG CTGCACAGTC CAAATGCCTG GACCAAGCCC TGGCCCTGTG GAAGGAGGTG 
CTTACTAAGG GGCAGGTGCC CGCTGTACGG TGTCTCCAGC AAACAGCAGC CTCCCTGCAG 
ATCCTAGCGG CCCTCTATCA GCTGGTGGCA AAGCCCCTGC AGGCTCTGGA GGTCCTCCTG 
CTCGTCCAGA TGGTCTCACA GAGACTGAAG GACCATGCGA AGGCAGCCGG CTCTTCCTGC 
CAAGTCACCC AGCTCCTCCT GACCCTCGGC TGTCCCAGCT ACGCCCAGGT GCACCTGGAA 
GAAGCAGAGT CGAGTCTGAA GCTTCTGGAT CGCACCACAG ACACATACCT GCTCCTCTCC 
CTGACCTGTG ACCTCCTACG AAGTCGACTC TGCTGCGCTC GCCAGAAGGT GGCTGAGGGT 
GCTGCTCTGC TGCTGTCTGT GCTTCAGGAC CCTGCCCTCC AGAGGGCATC CAAGGCCTGG 
TACTTGCTGC GTGTCCAGGC CCTGCAGCTG GTGGCAGCCT ACCTGAGTCT CTCGCCAGAC 
AGCCTGCTGG CCCCGCTGTG GGAGCAACTG TGTGCCCAAG GCTGGCAGAC ACCTGAGATA 
GCACTCATCG ACTCCCATAA GCTTCTCCGA AGCATCATCC TCCTGCTCAT GGGCAGCGAT 
GTGCTCTCAA TTCAGAAAAC AGCTGTGGAG ACGCCATTTC TGGACTACGG TGAAAATCTG 
GTACAAAAAT GGCAGGTTCT TTCTGAGGTG CTGAGCTGCT CGGAGAAGCT GGTCTCCCGC 
CTGGGCCGCC TTGGTAGTGT GAGCGAAGCC AAAGCCTTTT GCTTGGAGGC CCTGAAACTT 
ACAACAAAGC TGCAGATACC ACACCAGTGT GCCCTCTTCC TGGTGCTGAA GGGGGAGCTG 
GAGCTGGCAC GCAATGACAT GGACCTCTGT CAGTCGGACC TGCATCAAGT TCTGTTCTTG 
CTTGAGTCTT GCACAGAGTT TCTCGGACTA GCGCAGCACC CGGACTCTGT GAAGAAGGTC 
CACCTGCAGA AGAGGAAGCA GCAGGCTCGG GTGCCCCATG CTCCAGAGCT CCCGGACGAA 
GAGCTCATTC TGAAAGGCCC TGCCCTGGAG CTGGTGGCCA CCGTGGCCAA GGAACCTGGC 
CCTGTAGCAC CTTCTGCTAA CTCCTCCCCG ATCCTGAAAA CCAAGCCCCA GCCCAGCCCC 
AGCTTCCTGA CCCACTTGCC CACCTGTGAC TGCTCGCTCT GTGCCAGCCC TGTCCTCTCG 
GTGGTCTGTC TACGCTGGGC GTTGGTCACA GCGGGGCTGA GACTGGCCAT GGGCCATGAG 
GCCCAGGGGC TGGATCTGCT GCAGGTGGTG CTGAAGGGTT GCCCTGCAGC CAGTGGTCGC 
CTCGCCCAAG CTCTGCAGGC TTCCCTGAAT CACACAGCGC CCCCCTCCCC TGTCCCAAGC 
CTCTTTGATG AGATCTTGGC TCAGGCATAT ACACAGCTGG CACTGGAGGG GCTGAGCCAG 
CCATCAAACA AGAGCCTGGG AAAGGTCCTG GAGTCAGGGT TAAAGTTTGT GGTGGCGCGG 
ATACCCCACC TGGAGCCCTG GCGAGCCAGC CTGCTCTTGG TCCGGGCCCT TGCAAAGCTG 
ACCAGCCTCA GCTGCTGCAC TGCCCAACTT TTTGCAAGCT CCTGGGGCTG GCAGCCGCCA 
TCAATAAAGA ACCCCACAGG CTCAGAACCC TCTAAGCCTC GGAACCAAAA ACAGTCAGGA 
CAGTCCAGAC GAGGACGCCA GCAAGTGGGC TCTGCTACCC TGTCCCTCTC ACATACCTCT 
CTGAAAGGTC TGGAAGGTGA AGGACCACCC TGTACACCTA AGCCCCCAGG CCGGGTCAGG 
CAGGCCGGCC CTGGGGTACC CTTCACCGTA TTTGAGGAAG TCTTCCTTAC AAAGACCAAG 
CCTGAGGTTC CCAAGGCCCC CAGGGTACAA CGGAGGATCC AGACACGCCT CAAGGTGAAC 
TTCAGTGATG ACAGTGACTT GGAAGACCTT GTCTCAGTTG AAGCACAGCT TGCAGAGGGG 
TCCAAGAGAC AGGGCACTAC TTCCCGGGGC CGGGGCCGGG CCAGGAAGGG CCCGAGTGTG 
AAGACTAACT CAGTGGCCAC GTCAGGCAGT GCCCCCGGCC TCAGTGGCAG AAGCCGGAGG 
GTCAAGAAGG TGGCATCAGG ACATTGTGAG GAGCCGGGCC CTGAGATGAT GAGGACCATC 
CCTGAGGAAC TGACTGACAA CCAGATGGAA ATGAGCTTTG AGATCCTCAG GGGCTCTGAT 
GGGGAAGACT CAGCCTCAGG TGGGAAGACA CCAGGGCCTG ATTCAGCCGT AGGAGAATGT 
GAGGTGTTGA GACGGGATGC CAGCAAAGAG GAGCTGCCTG GCCCTGGCCC AGACAAGGAG 
AGAGACATGG ATCTGGGTCC CCGGCACAGA CTCCCCTCGG CCCCTGTAGC CATCGATCTG 
TCTACCCTGG ATTCCATCTG TGACTCACTA AGTGGTGCTT TCCGTGGCAT CAGTCACTGC 
CCTCCTAGTG GGCTCTATGC CCACCTCTGC CGCCTCCTGG CCTTGTGCCT GGGCCACCGG 
GATCCCTATG CCACTGCCTG CCTTGTCACC GAGTCTGTCT CCGTCACCTG TCGCCACCAG 
CTGCTTACCC ACCTCCACAG GCAGCTCAGC AAGGCCCAGA AGCACCGAGG GTCGCCAGAC 
ATAGCAGACC AGTTGCAGGG GCTGCACCTC CAGGAGAAGC CTGGAGACAT CCCTCTGGCA 
CGAATCCAGC GCCTCTTTGC CTTCAGGCCT TTGGGATCTG GCCACTTCCC CCAGCCTGAG 
AAGGACAGCT TCCAGGAGCG TCTTGCTCTG ATTCCCAGTG GGGTGACCGT GTGTGTGTTG 
GCCCTGGCCA CCCTCCAGCC TGGAACTGTG GGCAACACCC TCCTGCTGAC CCGGCTGGAA 
AAAGACAAAC CCCCGGTCAC TGTACAGATC CCCACTTCCC AGAGCAAGCT TTCTGTGAGT 
TCGGCCTTGA AAGAGTTTGA CGCCATCCAG AAGGAGCAGA AAGAGAACAG CAGCTGTACT 
GACAAGCGAG AATGGTGGAC TGGCCGGCTA GAGCTGGATC GCAGGATGGA GGTTCTCACC 
ACTTCCCTAG AGAAGTACGT GCTGGGCTGC TGGCGGGGGC TGTTGCTGCC ATCCTGCGAG 
GACCCCGACC TTGTCCGGGA GGCCTCCCAT CTACAAGACT CACTACAGGA ATGTGGCTGG 
AAATATCCTG ACCCCACTCT GCTGAGAATC ATGCTCAGCG GTGCCAGTAC CCTCACGCCG 
CAGGACGTGC AGGCCCTGGC TTATGGGCTG TGCCCAGGCC GACCAGCCCG AGCCCAAGAG 
CTCCTAGGTG AGGCAGTAGG GCGTCTCCAA GGTCAGACAT CAACAAGCAG TAGGCATCTC 
GTGTTAGTGC TGGACAAGGA CCTACAGAAG CTGCCGTGGG AGAGCATACC CAGCCTCCGG 
GCCCTGCCTG TCACTCGACT GCCCTCCTTC CGATTCCTAC TCAGCTACTC CATCATCAAA 
GAGTCTGGGG CCTCATCAGT GCTGAGCCAA GGAGTGGATC CTCGCAGAAC CTTCTACGTC 
CTGAACCCTC ACAATAACCT GTCAAGCACA GAGGAGCAAT TCCGAGCCCA TTTTAGCAGT 
GAAGCTGGCT GGAAAGGGGT GGTTGGGGAG GTGCCGAGCC CGGAACAGGT GCAAACGGCC 
TTGACGGAGC GTGACTTGTA CATCTATGCA GGGCATGGCG CCGGCGCCCG CTTCCTGGAC 
GGGCAGGCTG TCCTGCGGCT GAGCTGCCGG GCAGTGGCCC TGCTGTTCGG CTGCAGCAGC 
GCAGCCCTGG CTGTGCATGG AAACCTGGAG GGGGCTGGCA TTGTGCTCAA GTATATCATG 
GCTGGCTGCC CCCTGTTCCT AGGTAACCTC TGGGACGTGA CTGACCGTGA CATTGACCGC 
TACACAGAGG CTCTGTTGCA GGGCTGGCTT AGAGCAGGTT CAGGAGCCCC CCTTCTGTAC 
TATGTCAGCC AGGCCCGCCA GGCTCCCCGA CTCAAATACC TTATTGGGGC TGCACCTGTA 
GCCTATGGCT TGCCTGTCTC CCTGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ESPL1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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