ESPL1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESPL1
Entrez Gene ID	451937
Full Name	extra spindle pole bodies like 1, separase
General protein information	Preferred Names extra spindle pole bodies like 1, separase Names separin extra spindle pole bodies homolog 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016923344.1	XP_016778833.1	separin isoform X3
XM_016923345.1	XP_016778834.1	separin isoform X4
XM_016923342.1	XP_016778831.1	separin isoform X1
XM_016923343.1	XP_016778832.1	separin isoform X2
XM_016923341.1	XP_016778830.1	separin isoform X1
XM_016923341.2	XP_016778830.1	separin isoform X1
XM_016923342.2	XP_016778831.1	separin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ESPL1	NP_036423.4
Macaca mulatta (Rhesus monkey)	ESPL1	XP_001103494.1
Danio rerio (zebrafish)	espl1	XP_001337869.1
Pan troglodytes (chimpanzee)	ESPL1	XP_003313754.1
Canis lupus familiaris (dog)	ESPL1	XP_849828.2
Bos taurus (cattle)	ESPL1	NP_001039414.2
Gallus gallus (chicken)	ESPL1	XP_423289.4
Mus musculus (house mouse)	Espl1	NP_001014976.1
Rattus norvegicus (Norway rat)	Espl1	NP_001164073.1
Xenopus tropicalis (tropical clawed frog)	espl1	XP_004912005.1

Gene Ontology

The following ESPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc65809	XM_016923341.2 Latest version!	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc65809	XM_016923342.2 Latest version!	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc65810	XM_016923343.1 Latest version!	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc65811	XM_016923344.1 Latest version!	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc65812	XM_016923345.1 Latest version!	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OPc65809	XM_016923341.1	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc65809	XM_016923342.1	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESPL1 ( XM_016923342.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778831.1 Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

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CloneID	OPc65809
Clone ID Related Accession (Same CDS sequence)	XM_016923342.1 , XM_016923341.1 , XM_016923341.2 , XM_016923342.2
Accession Version	XM_016923342.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAAGTATG ATTTGTATAT CTATGCCGGG 
CATGGGGCTG GTGCCCGCTT CCTTGACGGG CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA 
GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG GCCCTGGCTG TGCATGGAAA CCTGGAGGGG 
GCTGGCATCG TGCTCAAGTA CATCATGGCT GGTTGCCCCT TGTTTCTGGG TAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACGGAAGCTC TGCTGCAAGG CTGGCTTGGA 
GCAGGCCCAG GGGCCCCCCT TCTCTACTAT GTAAACCAGG CCCGCCAAGC TCCCCGACTC 
AAGTATCTTA TTGGGGCTGC ACCTATAGCC TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778831.1
CDS	154..6510
Translation	MRSFKRVNFGTLLSSQKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSPKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRTQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASWGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKELPSPCPDKESDKDLGPRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQETPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKYDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_016923342.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923342.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAAGTATG ATTTGTATAT CTATGCCGGG 
CATGGGGCTG GTGCCCGCTT CCTTGACGGG CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA 
GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG GCCCTGGCTG TGCATGGAAA CCTGGAGGGG 
GCTGGCATCG TGCTCAAGTA CATCATGGCT GGTTGCCCCT TGTTTCTGGG TAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACGGAAGCTC TGCTGCAAGG CTGGCTTGGA 
GCAGGCCCAG GGGCCCCCCT TCTCTACTAT GTAAACCAGG CCCGCCAAGC TCCCCGACTC 
AAGTATCTTA TTGGGGCTGC ACCTATAGCC TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_016923341.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778830.1 Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

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CloneID	OPc65809
Clone ID Related Accession (Same CDS sequence)	XM_016923342.1 , XM_016923341.1 , XM_016923341.2 , XM_016923342.2
Accession Version	XM_016923341.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAAGTATG ATTTGTATAT CTATGCCGGG 
CATGGGGCTG GTGCCCGCTT CCTTGACGGG CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA 
GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG GCCCTGGCTG TGCATGGAAA CCTGGAGGGG 
GCTGGCATCG TGCTCAAGTA CATCATGGCT GGTTGCCCCT TGTTTCTGGG TAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACGGAAGCTC TGCTGCAAGG CTGGCTTGGA 
GCAGGCCCAG GGGCCCCCCT TCTCTACTAT GTAAACCAGG CCCGCCAAGC TCCCCGACTC 
AAGTATCTTA TTGGGGCTGC ACCTATAGCC TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778830.1
CDS	126..6482
Translation	MRSFKRVNFGTLLSSQKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSPKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRTQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASWGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKELPSPCPDKESDKDLGPRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQETPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKYDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_016923341.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923341.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAAGTATG ATTTGTATAT CTATGCCGGG 
CATGGGGCTG GTGCCCGCTT CCTTGACGGG CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA 
GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG GCCCTGGCTG TGCATGGAAA CCTGGAGGGG 
GCTGGCATCG TGCTCAAGTA CATCATGGCT GGTTGCCCCT TGTTTCTGGG TAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACGGAAGCTC TGCTGCAAGG CTGGCTTGGA 
GCAGGCCCAG GGGCCCCCCT TCTCTACTAT GTAAACCAGG CCCGCCAAGC TCCCCGACTC 
AAGTATCTTA TTGGGGCTGC ACCTATAGCC TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_016923341.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778830.1 Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

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CloneID	OPc65809
Clone ID Related Accession (Same CDS sequence)	XM_016923342.1 , XM_016923341.1 , XM_016923341.2 , XM_016923342.2
Accession Version	XM_016923341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016923341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAAGTATG ATTTGTATAT CTATGCCGGG 
CATGGGGCTG GTGCCCGCTT CCTTGACGGG CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA 
GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG GCCCTGGCTG TGCATGGAAA CCTGGAGGGG 
GCTGGCATCG TGCTCAAGTA CATCATGGCT GGTTGCCCCT TGTTTCTGGG TAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACGGAAGCTC TGCTGCAAGG CTGGCTTGGA 
GCAGGCCCAG GGGCCCCCCT TCTCTACTAT GTAAACCAGG CCCGCCAAGC TCCCCGACTC 
AAGTATCTTA TTGGGGCTGC ACCTATAGCC TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778830.1
CDS	156..6512
Translation	MRSFKRVNFGTLLSSQKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSPKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRTQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASWGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKELPSPCPDKESDKDLGPRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQETPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKYDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_016923341.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923341.2

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAAGTATG ATTTGTATAT CTATGCCGGG 
CATGGGGCTG GTGCCCGCTT CCTTGACGGG CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA 
GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG GCCCTGGCTG TGCATGGAAA CCTGGAGGGG 
GCTGGCATCG TGCTCAAGTA CATCATGGCT GGTTGCCCCT TGTTTCTGGG TAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACGGAAGCTC TGCTGCAAGG CTGGCTTGGA 
GCAGGCCCAG GGGCCCCCCT TCTCTACTAT GTAAACCAGG CCCGCCAAGC TCCCCGACTC 
AAGTATCTTA TTGGGGCTGC ACCTATAGCC TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_016923342.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778831.1 Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

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CloneID	OPc65809
Clone ID Related Accession (Same CDS sequence)	XM_016923342.1 , XM_016923341.1 , XM_016923341.2 , XM_016923342.2
Accession Version	XM_016923342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016923342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAAGTATG ATTTGTATAT CTATGCCGGG 
CATGGGGCTG GTGCCCGCTT CCTTGACGGG CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA 
GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG GCCCTGGCTG TGCATGGAAA CCTGGAGGGG 
GCTGGCATCG TGCTCAAGTA CATCATGGCT GGTTGCCCCT TGTTTCTGGG TAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACGGAAGCTC TGCTGCAAGG CTGGCTTGGA 
GCAGGCCCAG GGGCCCCCCT TCTCTACTAT GTAAACCAGG CCCGCCAAGC TCCCCGACTC 
AAGTATCTTA TTGGGGCTGC ACCTATAGCC TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778831.1
CDS	152..6508
Translation	MRSFKRVNFGTLLSSQKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSPKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRTQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASWGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKELPSPCPDKESDKDLGPRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQETPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKYDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_016923342.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923342.2

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAAGTATG ATTTGTATAT CTATGCCGGG 
CATGGGGCTG GTGCCCGCTT CCTTGACGGG CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA 
GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG GCCCTGGCTG TGCATGGAAA CCTGGAGGGG 
GCTGGCATCG TGCTCAAGTA CATCATGGCT GGTTGCCCCT TGTTTCTGGG TAATCTCTGG 
GATGTGACTG ACCGTGACAT TGACCGCTAC ACGGAAGCTC TGCTGCAAGG CTGGCTTGGA 
GCAGGCCCAG GGGCCCCCCT TCTCTACTAT GTAAACCAGG CCCGCCAAGC TCCCCGACTC 
AAGTATCTTA TTGGGGCTGC ACCTATAGCC TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_016923343.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778832.1 Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

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CloneID	OPc65810
Clone ID Related Accession (Same CDS sequence)	XM_016923343.1
Accession Version	XM_016923343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAACTATG CCGGGCATGG GGCTGGTGCC 
CGCTTCCTTG ACGGGCAGGC TGTCCTGCGG CTGAGCTGTC GGGCAGTGGC CCTGCTGTTT 
GGCTGTAGCA GTGCGGCCCT GGCTGTGCAT GGAAACCTGG AGGGGGCTGG CATCGTGCTC 
AAGTACATCA TGGCTGGTTG CCCCTTGTTT CTGGGTAATC TCTGGGATGT GACTGACCGT 
GACATTGACC GCTACACGGA AGCTCTGCTG CAAGGCTGGC TTGGAGCAGG CCCAGGGGCC 
CCCCTTCTCT ACTATGTAAA CCAGGCCCGC CAAGCTCCCC GACTCAAGTA TCTTATTGGG 
GCTGCACCTA TAGCCTATGG CTTGCCTGTC TCTCTGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778832.1
CDS	126..6467
Translation	MRSFKRVNFGTLLSSQKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSPKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRTQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASWGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKELPSPCPDKESDKDLGPRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQETPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTNYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_016923343.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923343.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGGACTT GCAGAAGCTG 
CCGTGGGAAA GCATGCCCAG CCTCCAGGCA CTGCCTGTCA CCCGGCTGCC CTCCTTCCGC 
TTCCTACTCA GCTACTCCAT CATCAAAGAG TATGGGGCCT CGCCAGTGCT GAGTCAAGGG 
GTGGATCCGC GAAGTACCTT CTATGTCCTG AACCCTCACA ATAACCTGTC AAGCACAGAG 
GAGCAATTTC GAGCCAATTT CAGCAGTGAA GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG 
CCAAGACCTG AACAGGTGCA GGAAGCCCTG ACAAACTATG CCGGGCATGG GGCTGGTGCC 
CGCTTCCTTG ACGGGCAGGC TGTCCTGCGG CTGAGCTGTC GGGCAGTGGC CCTGCTGTTT 
GGCTGTAGCA GTGCGGCCCT GGCTGTGCAT GGAAACCTGG AGGGGGCTGG CATCGTGCTC 
AAGTACATCA TGGCTGGTTG CCCCTTGTTT CTGGGTAATC TCTGGGATGT GACTGACCGT 
GACATTGACC GCTACACGGA AGCTCTGCTG CAAGGCTGGC TTGGAGCAGG CCCAGGGGCC 
CCCCTTCTCT ACTATGTAAA CCAGGCCCGC CAAGCTCCCC GACTCAAGTA TCTTATTGGG 
GCTGCACCTA TAGCCTATGG CTTGCCTGTC TCTCTGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_016923344.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778833.1 Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.

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CloneID	OPc65811
Clone ID Related Accession (Same CDS sequence)	XM_016923344.1
Accession Version	XM_016923344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	separin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGTATGG GGCCTCGCCA 
GTGCTGAGTC AAGGGGTGGA TCCGCGAAGT ACCTTCTATG TCCTGAACCC TCACAATAAC 
CTGTCAAGCA CAGAGGAGCA ATTTCGAGCC AATTTCAGCA GTGAAGCTGG CTGGAGAGGA 
GTGGTTGGGG AGGTGCCAAG ACCTGAACAG GTGCAGGAAG CCCTGACAAA GTATGATTTG 
TATATCTATG CCGGGCATGG GGCTGGTGCC CGCTTCCTTG ACGGGCAGGC TGTCCTGCGG 
CTGAGCTGTC GGGCAGTGGC CCTGCTGTTT GGCTGTAGCA GTGCGGCCCT GGCTGTGCAT 
GGAAACCTGG AGGGGGCTGG CATCGTGCTC AAGTACATCA TGGCTGGTTG CCCCTTGTTT 
CTGGGTAATC TCTGGGATGT GACTGACCGT GACATTGACC GCTACACGGA AGCTCTGCTG 
CAAGGCTGGC TTGGAGCAGG CCCAGGGGCC CCCCTTCTCT ACTATGTAAA CCAGGCCCGC 
CAAGCTCCCC GACTCAAGTA TCTTATTGGG GCTGCACCTA TAGCCTATGG CTTGCCTGTC 
TCTCTGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778833.1
CDS	126..6377
Translation	MRSFKRVNFGTLLSSQKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSPKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRTQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASWGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKELPSPCPDKESDKDLGPRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQETPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKYDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_016923344.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923344.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CCCTCAGCCC CCGTAGCCAC TGGTCTTTCT ACCCTGGACT CCATCTGTGA CTCCCTGAGT 
GTTGCTTTCC GGGGCATTAG TCACTGTCCT CCTAGTGGGC TCTATGCCCA CCTCTGCCGC 
TTCCTGGCCT TGTGCCTGGG CCACCGGGAT CCTTATGCCA CTGCTTTCCT TGTCACCGAG 
TCTGTCTCCA TCACCTGTCG CCACCAGCTG CTCACCCACC TCCACAGACA GCTCAGCAAG 
GCCCAGAAGC ACCGAGGATC ACTTGAAATA GCAGACCAGC TGCAGGGGCT GAGCCTTCAG 
GAGACGCCTG GAGATGTCCC CCTGGCCCGC ATCCAGCGCC TCTTTTCCTT CAGGGCTTTG 
GAATCTGGCC ACTTCCCCCA GCCTGAAAAG GAGAGTTTCC AGGAGCGCCT GGCTCTGATC 
CCCAGTGGGG TGACTGTGTG CGTGTTGGCC CTGGCCACCC TCCAGCCCGG AACGGTGGGC 
AACACCCTCC TGCTGACCCG GCTGGAAAAG GACAGTCCCC CAGTCAGTGT GCAGATTCCC 
ACTGGCCAGA ACAAGCTTCA TCTGCGTTCA GTCCTGAATG AGTTCGATGC CATCCAGAAG 
GCACAGAAAG AGAACAGCAG CTGTACTGAC AAGCGAGAAT GGTGGACAGG GCGGCTGGCA 
CTGGACCACA GGATGGAGGT TCTCATCGCT TCCCTAGAGA AGTCTGTGCT GGGCTGCTGG 
AAGGGGCTGC TGCTGCCGTC CAGTGAGGAG CCCGGCCCTG CCCAGGAGGC CTCCCGCCTA 
CAGGAGCTGC TACAGGACTG TGGCTGGAAA TATCCTGACC GCACTCTGCT GAAAATCATG 
CTCAGTGGTG CCGGTGCCCT CACCCCTCAG GACATTCAGG CCCTGGCCTA CGGGCTGTGC 
CCAACCCAGC CAGAGCGAGC CCAGGAGCTC CTGAATGAGG CAGTAGGACG TCTACAGGGC 
CTGACAGTAC CAAGCAATAG CCACCTTGTC TTGGTCCTAG ACAAGTATGG GGCCTCGCCA 
GTGCTGAGTC AAGGGGTGGA TCCGCGAAGT ACCTTCTATG TCCTGAACCC TCACAATAAC 
CTGTCAAGCA CAGAGGAGCA ATTTCGAGCC AATTTCAGCA GTGAAGCTGG CTGGAGAGGA 
GTGGTTGGGG AGGTGCCAAG ACCTGAACAG GTGCAGGAAG CCCTGACAAA GTATGATTTG 
TATATCTATG CCGGGCATGG GGCTGGTGCC CGCTTCCTTG ACGGGCAGGC TGTCCTGCGG 
CTGAGCTGTC GGGCAGTGGC CCTGCTGTTT GGCTGTAGCA GTGCGGCCCT GGCTGTGCAT 
GGAAACCTGG AGGGGGCTGG CATCGTGCTC AAGTACATCA TGGCTGGTTG CCCCTTGTTT 
CTGGGTAATC TCTGGGATGT GACTGACCGT GACATTGACC GCTACACGGA AGCTCTGCTG 
CAAGGCTGGC TTGGAGCAGG CCCAGGGGCC CCCCTTCTCT ACTATGTAAA CCAGGCCCGC 
CAAGCTCCCC GACTCAAGTA TCTTATTGGG GCTGCACCTA TAGCCTATGG CTTGCCTGTC 
TCTCTGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_016923345.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016778834.1 Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.

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CloneID	OPc65812
Clone ID Related Accession (Same CDS sequence)	XM_016923345.1
Accession Version	XM_016923345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	separin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTCT TTCTACCCTG 
GACTCCATCT GTGACTCCCT GAGTGTTGCT TTCCGGGGCA TTAGTCACTG TCCTCCTAGT 
GGGCTCTATG CCCACCTCTG CCGCTTCCTG GCCTTGTGCC TGGGCCACCG GGATCCTTAT 
GCCACTGCTT TCCTTGTCAC CGAGTCTGTC TCCATCACCT GTCGCCACCA GCTGCTCACC 
CACCTCCACA GACAGCTCAG CAAGGCCCAG AAGCACCGAG GATCACTTGA AATAGCAGAC 
CAGCTGCAGG GGCTGAGCCT TCAGGAGACG CCTGGAGATG TCCCCCTGGC CCGCATCCAG 
CGCCTCTTTT CCTTCAGGGC TTTGGAATCT GGCCACTTCC CCCAGCCTGA AAAGGAGAGT 
TTCCAGGAGC GCCTGGCTCT GATCCCCAGT GGGGTGACTG TGTGCGTGTT GGCCCTGGCC 
ACCCTCCAGC CCGGAACGGT GGGCAACACC CTCCTGCTGA CCCGGCTGGA AAAGGACAGT 
CCCCCAGTCA GTGTGCAGAT TCCCACTGGC CAGAACAAGC TTCATCTGCG TTCAGTCCTG 
AATGAGTTCG ATGCCATCCA GAAGGCACAG AAAGAGAACA GCAGCTGTAC TGACAAGCGA 
GAATGGTGGA CAGGGCGGCT GGCACTGGAC CACAGGATGG AGGTTCTCAT CGCTTCCCTA 
GAGAAGTCTG TGCTGGGCTG CTGGAAGGGG CTGCTGCTGC CGTCCAGTGA GGAGCCCGGC 
CCTGCCCAGG AGGCCTCCCG CCTACAGGAG CTGCTACAGG ACTGTGGCTG GAAATATCCT 
GACCGCACTC TGCTGAAAAT CATGCTCAGT GGTGCCGGTG CCCTCACCCC TCAGGACATT 
CAGGCCCTGG CCTACGGGCT GTGCCCAACC CAGCCAGAGC GAGCCCAGGA GCTCCTGAAT 
GAGGCAGTAG GACGTCTACA GGGCCTGACA GTACCAAGCA ATAGCCACCT TGTCTTGGTC 
CTAGACAAGG ACTTGCAGAA GCTGCCGTGG GAAAGCATGC CCAGCCTCCA GGCACTGCCT 
GTCACCCGGC TGCCCTCCTT CCGCTTCCTA CTCAGCTACT CCATCATCAA AGAGTATGGG 
GCCTCGCCAG TGCTGAGTCA AGGGGTGGAT CCGCGAAGTA CCTTCTATGT CCTGAACCCT 
CACAATAACC TGTCAAGCAC AGAGGAGCAA TTTCGAGCCA ATTTCAGCAG TGAAGCTGGC 
TGGAGAGGAG TGGTTGGGGA GGTGCCAAGA CCTGAACAGG TGCAGGAAGC CCTGACAAAG 
TATGATTTGT ATATCTATGC CGGGCATGGG GCTGGTGCCC GCTTCCTTGA CGGGCAGGCT 
GTCCTGCGGC TGAGCTGTCG GGCAGTGGCC CTGCTGTTTG GCTGTAGCAG TGCGGCCCTG 
GCTGTGCATG GAAACCTGGA GGGGGCTGGC ATCGTGCTCA AGTACATCAT GGCTGGTTGC 
CCCTTGTTTC TGGGTAATCT CTGGGATGTG ACTGACCGTG ACATTGACCG CTACACGGAA 
GCTCTGCTGC AAGGCTGGCT TGGAGCAGGC CCAGGGGCCC CCCTTCTCTA CTATGTAAAC 
CAGGCCCGCC AAGCTCCCCG ACTCAAGTAT CTTATTGGGG CTGCACCTAT AGCCTATGGC 
TTGCCTGTCT CTCTGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016778834.1
CDS	126..6326
Translation	MRSFKRVNFGTLLSSQKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSPKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRTQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASWGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQETPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKYDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_016923345.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016923345.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGAAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GAGGCTCCCC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CAGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAACCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCTGGG GCCGGGGGCG AGCAAGGAAG GGTCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTCT TTCTACCCTG 
GACTCCATCT GTGACTCCCT GAGTGTTGCT TTCCGGGGCA TTAGTCACTG TCCTCCTAGT 
GGGCTCTATG CCCACCTCTG CCGCTTCCTG GCCTTGTGCC TGGGCCACCG GGATCCTTAT 
GCCACTGCTT TCCTTGTCAC CGAGTCTGTC TCCATCACCT GTCGCCACCA GCTGCTCACC 
CACCTCCACA GACAGCTCAG CAAGGCCCAG AAGCACCGAG GATCACTTGA AATAGCAGAC 
CAGCTGCAGG GGCTGAGCCT TCAGGAGACG CCTGGAGATG TCCCCCTGGC CCGCATCCAG 
CGCCTCTTTT CCTTCAGGGC TTTGGAATCT GGCCACTTCC CCCAGCCTGA AAAGGAGAGT 
TTCCAGGAGC GCCTGGCTCT GATCCCCAGT GGGGTGACTG TGTGCGTGTT GGCCCTGGCC 
ACCCTCCAGC CCGGAACGGT GGGCAACACC CTCCTGCTGA CCCGGCTGGA AAAGGACAGT 
CCCCCAGTCA GTGTGCAGAT TCCCACTGGC CAGAACAAGC TTCATCTGCG TTCAGTCCTG 
AATGAGTTCG ATGCCATCCA GAAGGCACAG AAAGAGAACA GCAGCTGTAC TGACAAGCGA 
GAATGGTGGA CAGGGCGGCT GGCACTGGAC CACAGGATGG AGGTTCTCAT CGCTTCCCTA 
GAGAAGTCTG TGCTGGGCTG CTGGAAGGGG CTGCTGCTGC CGTCCAGTGA GGAGCCCGGC 
CCTGCCCAGG AGGCCTCCCG CCTACAGGAG CTGCTACAGG ACTGTGGCTG GAAATATCCT 
GACCGCACTC TGCTGAAAAT CATGCTCAGT GGTGCCGGTG CCCTCACCCC TCAGGACATT 
CAGGCCCTGG CCTACGGGCT GTGCCCAACC CAGCCAGAGC GAGCCCAGGA GCTCCTGAAT 
GAGGCAGTAG GACGTCTACA GGGCCTGACA GTACCAAGCA ATAGCCACCT TGTCTTGGTC 
CTAGACAAGG ACTTGCAGAA GCTGCCGTGG GAAAGCATGC CCAGCCTCCA GGCACTGCCT 
GTCACCCGGC TGCCCTCCTT CCGCTTCCTA CTCAGCTACT CCATCATCAA AGAGTATGGG 
GCCTCGCCAG TGCTGAGTCA AGGGGTGGAT CCGCGAAGTA CCTTCTATGT CCTGAACCCT 
CACAATAACC TGTCAAGCAC AGAGGAGCAA TTTCGAGCCA ATTTCAGCAG TGAAGCTGGC 
TGGAGAGGAG TGGTTGGGGA GGTGCCAAGA CCTGAACAGG TGCAGGAAGC CCTGACAAAG 
TATGATTTGT ATATCTATGC CGGGCATGGG GCTGGTGCCC GCTTCCTTGA CGGGCAGGCT 
GTCCTGCGGC TGAGCTGTCG GGCAGTGGCC CTGCTGTTTG GCTGTAGCAG TGCGGCCCTG 
GCTGTGCATG GAAACCTGGA GGGGGCTGGC ATCGTGCTCA AGTACATCAT GGCTGGTTGC 
CCCTTGTTTC TGGGTAATCT CTGGGATGTG ACTGACCGTG ACATTGACCG CTACACGGAA 
GCTCTGCTGC AAGGCTGGCT TGGAGCAGGC CCAGGGGCCC CCCTTCTCTA CTATGTAAAC 
CAGGCCCGCC AAGCTCCCCG ACTCAAGTAT CTTATTGGGG CTGCACCTAT AGCCTATGGC 
TTGCCTGTCT CTCTGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ESPL1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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