emilin1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	emilin1a
Entrez Gene ID	566473
Full Name	elastin microfibril interfacer 1a
Synonyms	im:7159005,si:dkey-20n10.2
General protein information	Preferred Names elastin microfibril interfacer 1a Names EMILIN-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160756.2	XP_005160813.1	EMILIN-1 isoform X1
NM_001030207.1	NP_001025378.1	EMILIN-1 precursor
XM_005160756.3	XP_005160813.1	EMILIN-1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	EMILIN1	NP_001179363.1
Mus musculus (house mouse)	Emilin1	NP_598679.1
Pan troglodytes (chimpanzee)	EMILIN1	XP_515351.2
Macaca mulatta (Rhesus monkey)	EMILIN1	XP_001089257.1
Rattus norvegicus (Norway rat)	Emilin1	NP_001100180.1
Homo sapiens (human)	EMILIN1	NP_008977.1
Canis lupus familiaris (dog)	EMILIN1	XP_003432201.1
Danio rerio (zebrafish)	emilin1a	NP_001025378.1
Xenopus tropicalis (tropical clawed frog)	emilin1	NP_001090870.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic control of cellular morphogenesis in Müller glia.
Charlton-Perkins M, Almeida AD, MacDonald RB, Harris WA
Glia67(7)1401-1411(2019 07)

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Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Genetic compensation induced by deleterious mutations but not gene knockdowns.
Rossi A, Kontarakis Z, Gerri C, Nolte H, Hölper S, Krüger M, Stainier DY
Nature524(7564)230-3(2015 Aug)

Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators.
Tijssen MR, Cvejic A, Joshi A, Hannah RL, Ferreira R, Forrai A, Bellissimo DC, Oram SH, Smethurst PA, Wilson NK, Wang X, Ottersbach K, Stemple DL, Green AR, Ouwehand WH, Göttgens B
Developmental cell20(5)597-609(2011 May)

Characterization of C1q in teleosts: insight into the molecular and functional evolution of C1q family and classical pathway.
Hu YL, Pan XM, Xiang LX, Shao JZ
The Journal of biological chemistry285(37)28777-86(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following emilin1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emilin1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33878	NM_001030207.1 Latest version!	Danio rerio elastin microfibril interfacer 1a (emilin1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa25031	XM_005160756.3 Latest version!	Danio rerio elastin microfibril interfacer 1a (emilin1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa25031	XM_005160756.2	Danio rerio elastin microfibril interfacer 1a (emilin1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

emilin1a ( NM_001030207.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025378.1 Danio rerio elastin microfibril interfacer 1a (emilin1a), mRNA.

Price & Availability↑

CloneID	ODa33878
Clone ID Related Accession (Same CDS sequence)	NM_001030207.1
Accession Version	NM_001030207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570809600000
Organism	Danio rerio(zebrafish)
Product	EMILIN-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX465184.7. On Aug 6, 2005 this sequence version replaced XM_689743.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCACTAT ATTTTGTTTA CTTGTCCACT CTATTGGCGC TGATTTTGCT TGGAGATAAT 
TGGGCAGCGG GAACTTACGC TTCTCGATAC ACTCAACATG TGGATGAGAA TCAGTCTGGA 
GCAGCAGCAC AGAGTGGATC TAAGGTGACC AGCAGACATA GGAACTGGTG TGCCTACGTA 
GTGACGCGGA CTGTAAGCTG TGTTATGGAA GATGGAGTGG AAACTTATGT CAAACCTGAA 
TACCAGCGCT GTGCTTGGGG CCAATGTTCC CATGTGGTTT TGTATCGGAC TTACAGAAAA 
CCACGGTATA AGGTTGCCTA TAAAGTGGTA TCAGAGATGG AATGGAAGTG CTGTCATGGT 
TACTCAGGTG ATGACTGCAG TGACGGTTCC TCTGCCATAC ATGACAGCAG GGCAAGACCT 
ACTGGTGAAG AAGGGAGAAG TGACAGCGAC AGGATTAGAC AACTCGAGGA ACAAATACAA 
AGCTTGAACA AAAACCTTCA CAACCTCCAA AAGAAAATAT ATGAAGAATC TCAAAGGGAA 
GGCATCAGCG GTGGCAACAA TCTGGCAGAC GCTGCCCAGC CAGGGATGAA AGAGACCATT 
CACAGCATCC AGACCAAACT GGACATGCTG GACAACATGA CACGAGTGCA TGACAAGACC 
CTCACAAACA TCAACAACCA CCTTGTGGGC GGCAATGGAA TTGAGAATGA GTTGGACAGT 
CGCTATGGCA CACTTAAAGA GGAGATTCTA CGGGAGCTTG AACGGCGGGT GACGCTGTCT 
TGTTCGTCTT GCCAAACCGG AGTGGAGAGC ATACAAAGGC AACATCAGGA AGACAGAGAG 
AGGATCCGAG AACTGGAGAA GCACATCAGT GTGATGGAAC AGCACCACCA GCAAACCTTA 
GACTTGCTAT CACGCTCTCA GAGTTGCTGT GATTCCCTGG ACAGGAAGCT CAGTGCAATT 
GACAGAAAGG TAAGTTCGAC TGCAGAGACA TACGATATCC TTAGAGGACG CCTTGAGAAG 
GAGCTGAGAA GTAATGGCAA TGGAGGTCGT GGAAAAGCTA TGGAGGAAAA ACTGAATAAC 
CGCCTTCGAG ACCTGGAGCG GAGATTGAAT GGGACTGTGA GAAAAACTGA GCAAAAATGC 
TCCCATACAG AGACAAGTAT GAAGGAGTTT GTCCAGAGAG AGATCGGCCA GATAAAGAAC 
TCAGTCCTCG GTCGAAACGA TGATCATGGT TATAGGATAT CTACAGTTGA GATAGACATC 
CAAGACTTGA AGCGTTTTAT TAACGATCAT AAAAATAACC TGGAACGGTT AGGGAACAAA 
ACAAACGATC TTGACAGTGG GCTTAAGTCA GCGATTCATT TGTGCACAGA GACATGCGCT 
GCCAAAGGGA GTGAGACAGA AGATACTGTG AAAAGCCTGG AATGGAAGGT TGTTGCCAAT 
GAGGAGGACA TTAAGAAGTT TGACACCAAG CTAAAGGATA TAAGTGTGTC TGGTGACTCC 
TTATTGGACC GTGTAATAGA TCTAAGCAAT GACGTTCAAA AAATTAAGGA TCTGACAGGA 
CAAAATGGAG AACACTTTAA CCAAATCGTC ACCGATGTGG AGAATCTGGG ACGTGATTGT 
GACGTATGCA GCTCGATAGA TGGCGAACTA CAAAAAATAA GAAACGTCAC GAGCTACGCT 
TTCGACAAAT TCCAGGCAGA GCTTACAGTT TTGGGCAGAA AGGTATTTTC AGATGAGCAC 
GTTTGCTCAC AGGTGTGCTC AAACCTCCAG GAGGAAGTGG GCAAATTGAA AGAAGAGGTT 
GAAAAATGCA CAGGCCAATG CATGATCAGT ATGGCTGACC ATCAGAGAAA TGTAGACAAT 
CAAAATACTG TAACTCGCAA GCTTGGCAAA GACCTTAAAT CTATTCAAGG TGAGCTTACA 
GGGATTCTCC AAATATTCAA CTCCATCAAC GATACTCTAA AAGGCCTCAG GAACACCATA 
CAGAAGCATG GCAACAATAT TACTGATCTG AACACGTCTA AGGGCAAAAT CTACTTGGAG 
TTGGATCAGC TTTATGATGA TCTGAACAAG CACAAAGTGG ACAGCAAAGG ACATTTCGAC 
AGCATCAGTA GCTTTAACAG CAACTTGATG ACTGAAATGG GAGAATGCAG ACTTTCTCGA 
GAGGGCCTGG ATAAAAGGCT TTCAAAGATG GAAGATGTCT GCGGTAGACT GGATACTTTG 
TCAGAGAACC TTAAGACAAT TAAAGATGGC CTGAGCAAAC ACGTCTCTGG GCTTTGGACT 
TGTGTTAATG ATCTAAACTC CACGGTTATC TCTCACAGTG AGACCATTAG CCGCATTCAT 
AATGTTCACT TGGAGAACAT TCAAGGCCGG ATGAACAATC TTAACTCTTC CATACGGAAT 
GTTCTCAAGG AGTTCCAGAT CTTCACTGAA CAGGACTTTA CTGGACCTCC AGGTCTGCCA 
GGTCCTCAAG GAGAGAAAGG ATCTAAAGGA CCACCTGGGC CACGAGGACC TCTAGGAAAG 
GAAGGCCCAC AAGGGAGAGT GGGGCCGGTA GGACCCCCAG GACCTCCTGG AAAGGATGCC 
AATGTTCCAA GACTGTCGTT CTCAGCAGCC CTCACAAGAC CCCAAGCCAG TTCAGGCACA 
ATTATTTTCA ACAAGGTCTT CATCAATGAA AGAAAGGCCT ACAATCCAAA AACAGGTGTG 
TTCACTGCTC CAGTGAGGGG GCGATACTTC TTCAGTGCAG TCTTGACAGG TCACAAAAAT 
GTCAAGATAG AGGCGGTTTT ATCTAAATCC AATTTTGGCA TTGCCCGTGG AGATTCAGCA 
GGCTATCAGC CCGAGGGTCT TGAGAAGCCC ATGGCTGAAG CACGGCACAC TCCAGGTTCT 
CTGGTCATTT TTAATATCGT ACTTCCTCTG CAGGAGGGAG ATACAATCTG CATTGATTTG 
GTCACAGGTA AACTTGCCCA CTCCGTGGAG CCGCTCACCA TCTTCAGTGG GATGTTGCTC 
TATGAGGAAT CAGAGGACAG GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025378.1
CDS	1..3027
Translation	MALYFVYLSTLLALILLGDNWAAGTYASRYTQHVDENQSGAAAQSGSKVTSRHRNWCAYVVTRTVSCVMEDGVETYVKPEYQRCAWGQCSHVVLYRTYRKPRYKVAYKVVSEMEWKCCHGYSGDDCSDGSSAIHDSRARPTGEEGRSDSDRIRQLEEQIQSLNKNLHNLQKKIYEESQREGISGGNNLADAAQPGMKETIHSIQTKLDMLDNMTRVHDKTLTNINNHLVGGNGIENELDSRYGTLKEEILRELERRVTLSCSSCQTGVESIQRQHQEDRERIRELEKHISVMEQHHQQTLDLLSRSQSCCDSLDRKLSAIDRKVSSTAETYDILRGRLEKELRSNGNGGRGKAMEEKLNNRLRDLERRLNGTVRKTEQKCSHTETSMKEFVQREIGQIKNSVLGRNDDHGYRISTVEIDIQDLKRFINDHKNNLERLGNKTNDLDSGLKSAIHLCTETCAAKGSETEDTVKSLEWKVVANEEDIKKFDTKLKDISVSGDSLLDRVIDLSNDVQKIKDLTGQNGEHFNQIVTDVENLGRDCDVCSSIDGELQKIRNVTSYAFDKFQAELTVLGRKVFSDEHVCSQVCSNLQEEVGKLKEEVEKCTGQCMISMADHQRNVDNQNTVTRKLGKDLKSIQGELTGILQIFNSINDTLKGLRNTIQKHGNNITDLNTSKGKIYLELDQLYDDLNKHKVDSKGHFDSISSFNSNLMTEMGECRLSREGLDKRLSKMEDVCGRLDTLSENLKTIKDGLSKHVSGLWTCVNDLNSTVISHSETISRIHNVHLENIQGRMNNLNSSIRNVLKEFQIFTEQDFTGPPGLPGPQGEKGSKGPPGPRGPLGKEGPQGRVGPVGPPGPPGKDANVPRLSFSAALTRPQASSGTIIFNKVFINERKAYNPKTGVFTAPVRGRYFFSAVLTGHKNVKIEAVLSKSNFGIARGDSAGYQPEGLEKPMAEARHTPGSLVIFNIVLPLQEGDTICIDLVTGKLAHSVEPLTIFSGMLLYEESEDRL

Target ORF information:

RefSeq Version	NM_001030207.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio elastin microfibril interfacer 1a (emilin1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030207.1

ORF Insert Sequence:

ATGGCACTAT ATTTTGTTTA CTTGTCCACT CTATTGGCGC TGATTTTGCT TGGAGATAAT 
TGGGCAGCGG GAACTTACGC TTCTCGATAC ACTCAACATG TGGATGAGAA TCAGTCTGGA 
GCAGCAGCAC AGAGTGGATC TAAGGTGACC AGCAGACATA GGAACTGGTG TGCCTACGTA 
GTGACGCGGA CTGTAAGCTG TGTTATGGAA GATGGAGTGG AAACTTATGT CAAACCTGAA 
TACCAGCGCT GTGCTTGGGG CCAATGTTCC CATGTGGTTT TGTATCGGAC TTACAGAAAA 
CCACGGTATA AGGTTGCCTA TAAAGTGGTA TCAGAGATGG AATGGAAGTG CTGTCATGGT 
TACTCAGGTG ATGACTGCAG TGACGGTTCC TCTGCCATAC ATGACAGCAG GGCAAGACCT 
ACTGGTGAAG AAGGGAGAAG TGACAGCGAC AGGATTAGAC AACTCGAGGA ACAAATACAA 
AGCTTGAACA AAAACCTTCA CAACCTCCAA AAGAAAATAT ATGAAGAATC TCAAAGGGAA 
GGCATCAGCG GTGGCAACAA TCTGGCAGAC GCTGCCCAGC CAGGGATGAA AGAGACCATT 
CACAGCATCC AGACCAAACT GGACATGCTG GACAACATGA CACGAGTGCA TGACAAGACC 
CTCACAAACA TCAACAACCA CCTTGTGGGC GGCAATGGAA TTGAGAATGA GTTGGACAGT 
CGCTATGGCA CACTTAAAGA GGAGATTCTA CGGGAGCTTG AACGGCGGGT GACGCTGTCT 
TGTTCGTCTT GCCAAACCGG AGTGGAGAGC ATACAAAGGC AACATCAGGA AGACAGAGAG 
AGGATCCGAG AACTGGAGAA GCACATCAGT GTGATGGAAC AGCACCACCA GCAAACCTTA 
GACTTGCTAT CACGCTCTCA GAGTTGCTGT GATTCCCTGG ACAGGAAGCT CAGTGCAATT 
GACAGAAAGG TAAGTTCGAC TGCAGAGACA TACGATATCC TTAGAGGACG CCTTGAGAAG 
GAGCTGAGAA GTAATGGCAA TGGAGGTCGT GGAAAAGCTA TGGAGGAAAA ACTGAATAAC 
CGCCTTCGAG ACCTGGAGCG GAGATTGAAT GGGACTGTGA GAAAAACTGA GCAAAAATGC 
TCCCATACAG AGACAAGTAT GAAGGAGTTT GTCCAGAGAG AGATCGGCCA GATAAAGAAC 
TCAGTCCTCG GTCGAAACGA TGATCATGGT TATAGGATAT CTACAGTTGA GATAGACATC 
CAAGACTTGA AGCGTTTTAT TAACGATCAT AAAAATAACC TGGAACGGTT AGGGAACAAA 
ACAAACGATC TTGACAGTGG GCTTAAGTCA GCGATTCATT TGTGCACAGA GACATGCGCT 
GCCAAAGGGA GTGAGACAGA AGATACTGTG AAAAGCCTGG AATGGAAGGT TGTTGCCAAT 
GAGGAGGACA TTAAGAAGTT TGACACCAAG CTAAAGGATA TAAGTGTGTC TGGTGACTCC 
TTATTGGACC GTGTAATAGA TCTAAGCAAT GACGTTCAAA AAATTAAGGA TCTGACAGGA 
CAAAATGGAG AACACTTTAA CCAAATCGTC ACCGATGTGG AGAATCTGGG ACGTGATTGT 
GACGTATGCA GCTCGATAGA TGGCGAACTA CAAAAAATAA GAAACGTCAC GAGCTACGCT 
TTCGACAAAT TCCAGGCAGA GCTTACAGTT TTGGGCAGAA AGGTATTTTC AGATGAGCAC 
GTTTGCTCAC AGGTGTGCTC AAACCTCCAG GAGGAAGTGG GCAAATTGAA AGAAGAGGTT 
GAAAAATGCA CAGGCCAATG CATGATCAGT ATGGCTGACC ATCAGAGAAA TGTAGACAAT 
CAAAATACTG TAACTCGCAA GCTTGGCAAA GACCTTAAAT CTATTCAAGG TGAGCTTACA 
GGGATTCTCC AAATATTCAA CTCCATCAAC GATACTCTAA AAGGCCTCAG GAACACCATA 
CAGAAGCATG GCAACAATAT TACTGATCTG AACACGTCTA AGGGCAAAAT CTACTTGGAG 
TTGGATCAGC TTTATGATGA TCTGAACAAG CACAAAGTGG ACAGCAAAGG ACATTTCGAC 
AGCATCAGTA GCTTTAACAG CAACTTGATG ACTGAAATGG GAGAATGCAG ACTTTCTCGA 
GAGGGCCTGG ATAAAAGGCT TTCAAAGATG GAAGATGTCT GCGGTAGACT GGATACTTTG 
TCAGAGAACC TTAAGACAAT TAAAGATGGC CTGAGCAAAC ACGTCTCTGG GCTTTGGACT 
TGTGTTAATG ATCTAAACTC CACGGTTATC TCTCACAGTG AGACCATTAG CCGCATTCAT 
AATGTTCACT TGGAGAACAT TCAAGGCCGG ATGAACAATC TTAACTCTTC CATACGGAAT 
GTTCTCAAGG AGTTCCAGAT CTTCACTGAA CAGGACTTTA CTGGACCTCC AGGTCTGCCA 
GGTCCTCAAG GAGAGAAAGG ATCTAAAGGA CCACCTGGGC CACGAGGACC TCTAGGAAAG 
GAAGGCCCAC AAGGGAGAGT GGGGCCGGTA GGACCCCCAG GACCTCCTGG AAAGGATGCC 
AATGTTCCAA GACTGTCGTT CTCAGCAGCC CTCACAAGAC CCCAAGCCAG TTCAGGCACA 
ATTATTTTCA ACAAGGTCTT CATCAATGAA AGAAAGGCCT ACAATCCAAA AACAGGTGTG 
TTCACTGCTC CAGTGAGGGG GCGATACTTC TTCAGTGCAG TCTTGACAGG TCACAAAAAT 
GTCAAGATAG AGGCGGTTTT ATCTAAATCC AATTTTGGCA TTGCCCGTGG AGATTCAGCA 
GGCTATCAGC CCGAGGGTCT TGAGAAGCCC ATGGCTGAAG CACGGCACAC TCCAGGTTCT 
CTGGTCATTT TTAATATCGT ACTTCCTCTG CAGGAGGGAG ATACAATCTG CATTGATTTG 
GTCACAGGTA AACTTGCCCA CTCCGTGGAG CCGCTCACCA TCTTCAGTGG GATGTTGCTC 
TATGAGGAAT CAGAGGACAG GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emilin1a ( XM_005160756.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160813.1 Danio rerio elastin microfibril interfacer 1a (emilin1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25031
Clone ID Related Accession (Same CDS sequence)	XM_005160756.2 , XM_005160756.3
Accession Version	XM_005160756.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	EMILIN-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005160756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTAT ATTTTGTTTA CTTGTCCACT CTATTGGCGC TGATTTTGCT TGGAGATAAT 
TGGGCAGCGG GAACTTACGC TTCTCGATAC ACTCAACATG TGGATGAGAA TCAGTCTGGA 
GCAGCAGCAC AGAGTGGATC TAAGGTGACC AGCAGACATA GGAACTGGTG TGCCTACGTA 
GTGACGCGGA CTGTAAGCTG TGTTATGGAA GATGGAGTGG AAACTTATGT CAAACCTGAA 
TACCAGCGCT GTGCTTGGGG CCAATGTTCC CATGTGGTTT TGTATCGGAC TTACAGAAAA 
CCACGGTATA AGGTTGCCTA TAAAGTGGTA TCAGAGATGG AATGGAAGTG CTGTCATGGT 
TACTCAGGTG ATGACTGCAG TGACGGTTCC TCTGCCATAC ATGACAGCAG GGCAAGACCT 
ACTGGTGAAG AAGGGAGAAG TGACAGCGAC AGGATTAGAC AACTCGAGGA ACAAATACAA 
AGCTTGAACA AAAACCTTCA CAACCTCCAA AAGAAAATAT ATGAAGAATC TCAAAGGGAA 
GGCATCAGCG GTGGCAACAA TCTGGCAGAC GCTGCCCAGC CAGGGATGAA AGAGACCATT 
CACAGCATCC AGACCAAACT GGACATGCTG GACAACATGA CACGAGTGCA TGACAAGACC 
CTCACAAACA TCAACAACCA CCTTGTGGGC GGCAATGGAA TTGAGAATGA GTTGGACAGT 
CGCTATGGCA CACTTAAAGA GGAGATTCTA CGGGAGCTTG AACGGCGGGT GACGCTGTCT 
TGTTCGTCTT GCCAAACCGG AGTGGAGAGC ATACAAAGGC AACATCAGGA AGACAGAGAG 
AGGATCCGAG AACTGGAGAA GCACATCAGT GTGATGGAAC AGCACCACCA GCAAACCTTA 
GACTTGCTAT CACGCTCTCA GAGTTGCTGT GATTCCCTGG ACAGGAAGCT CAGTGCAATT 
GACAGAAAGG TAAGTTCGAC TGCAGAGACA TACGATATCC TTAGAGGACG CCTTGAGAAG 
GAGCTGAGAA GTAATGGCAA TGGAGGTCGT GGAAAAGCTA TGGAGGAAAA ACTGAATAAC 
CGCCTTCGAG ACCTGGAGCG GAGATTGAAT GGGACTGTGA GAAAAACTGA GCAAAAATGC 
TCCCATACAG AGACAAGTAT GAAGGAGTTT GTCCAGAGAG AGATCGGCCA GATAAAGAAC 
TCAGTCCTCG GTCGAAACGA TGATCATGGT TATAGGATAT CTACAGTTGA GATAGACATC 
CAAGACTTGA AGCGTTTTAT TAACGATCAT AAAAATAACC TGGAACGGTT AGGGAACAAA 
ACAAACGATC TTGACAGTGG GCTTAAGTCA GCGATTCATT TGTGCACAGA GACATGCGCT 
GCCAAAGGGA GTGAGACAGA AGATACTGTG AAAAGCCTGG AATGGAAGGT TGTTGCCAAT 
GAGGAGGACA TTAAGAAGTT TGACACCAAG CTAAAGGATA TAAGTGTGTC TGGTGACTCC 
TTATTGGACC GTGTAATAGA TCTAAGCAAT GACGTTCAAA AAATTAAGGA TCTGACAGGA 
CAAAATGGAG AACACTTTAA CCAAATCGTC ACCGATGTGG AGAATCTGGG ACGTGATTGT 
GACGTATGCA GCTCGATAGA TGGCGAACTA CAAAAAATAA GAAACGTCAC GAGCTACGCT 
TTCGACAAAT TCCAGGCAGA GCTTACAGTT TTGGGCAGAA AGGTATTTTC AGATGAGCAC 
GTTTGCTCAC AGGTGTGCTC AAACCTCCAG GAGGAAGTGG GCAAATTGAA AGAAGAGGTT 
GAAAAATGCA CAGGCCAATG CATGATCAGT ATGGCTGACC ATCAGAGAAA TGTAGACAAT 
CAAAATACTG TAACTCGCAA GCTTGGCAAA GACCTTAAAT CTATTCAAGG TGAGCTTACA 
GGGATTCTCC AAATATTCAA CTCCATCAAC GATACTCTAA AAGGCCTCAG GAACACCATA 
CAGAAGCATG GCAACAATAT TACTGATCTG AACACGTCTA AGGGCAAAAT CTACTTGGAG 
TTGGATCAGC TTTATGATGA TCTGAACAAG CACAAAGTGG ACAGCAAAGG ACATTTCGAC 
AGCATCAGTA GCTTTAACAG CAACTTGATG ACTGAAATGG GAGAATGCAG ACTTTCTCGA 
GAGGGCCTGG ATAAAAGGCT TTCAAAGATG GAAGATGTCT GCGGTAGACT GGATACTTTG 
TCAGAGAACC TTAAGACAAT TAAAGATGGC CTGAGCAAAC ACGTCTCTGG GCTTTGGACT 
TGTGTTAATG ATCTAAACTC CACGGTTATC TCTCACAGTG AGACCATTAG CCGCATTCAT 
AATGTTCACT TGGAGAACAT TCAAGGCCGG ATGAACAATC TTAACTCTTC CATACGGAAT 
GTTCTCAAGG AGTTCCAGAT CTTCACTGAA CAGGACTTTA CTGGACCTCC AGGTCTGCCA 
GGTCCTCAAG GAGAGAAAGG ATCTAAAGGA CCACCTGGGC CACGAGGACC TCTAGGAAAG 
GAAGGCCCAC AAGGGAGAGT GGGGCCGGTA GGACCCCCAG GGCTCAGAGG TGAACAAGGA 
CCTCCTGGAA AGGATGCCAA TGTTCCAAGA CTGTCGTTCT CAGCAGCCCT CACAAGACCC 
CAAGCCAGTT CAGGCACAAT TATTTTCAAC AAGGTCTTCA TCAATGAAAG AAAGGCCTAC 
AATCCAAAAA CAGGTGTGTT CACTGCTCCA GTGAGGGGGC GATACTTCTT CAGTGCAGTC 
TTGACAGGTC ACAAAAATGT CAAGATAGAG GCGGTTTTAT CTAAATCCAA TTTTGGCATT 
GCCCGTGGAG ATTCAGCAGG CTATCAGCCC GAGGGTCTTG AGAAGCCCAT GGCTGAAGCA 
CGGCACACTC CAGGTTCTCT GGTCATTTTT AATATCGTAC TTCCTCTGCA GGAGGGAGAT 
ACAATCTGCA TTGATTTGGT CACAGGTAAA CTTGCCCACT CCGTGGAGCC GCTCACCATC 
TTCAGTGGGA TGTTGCTCTA TGAGGAATCA GAGGACAGGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160813.1
CDS	339..3383
Translation	MALYFVYLSTLLALILLGDNWAAGTYASRYTQHVDENQSGAAAQSGSKVTSRHRNWCAYVVTRTVSCVMEDGVETYVKPEYQRCAWGQCSHVVLYRTYRKPRYKVAYKVVSEMEWKCCHGYSGDDCSDGSSAIHDSRARPTGEEGRSDSDRIRQLEEQIQSLNKNLHNLQKKIYEESQREGISGGNNLADAAQPGMKETIHSIQTKLDMLDNMTRVHDKTLTNINNHLVGGNGIENELDSRYGTLKEEILRELERRVTLSCSSCQTGVESIQRQHQEDRERIRELEKHISVMEQHHQQTLDLLSRSQSCCDSLDRKLSAIDRKVSSTAETYDILRGRLEKELRSNGNGGRGKAMEEKLNNRLRDLERRLNGTVRKTEQKCSHTETSMKEFVQREIGQIKNSVLGRNDDHGYRISTVEIDIQDLKRFINDHKNNLERLGNKTNDLDSGLKSAIHLCTETCAAKGSETEDTVKSLEWKVVANEEDIKKFDTKLKDISVSGDSLLDRVIDLSNDVQKIKDLTGQNGEHFNQIVTDVENLGRDCDVCSSIDGELQKIRNVTSYAFDKFQAELTVLGRKVFSDEHVCSQVCSNLQEEVGKLKEEVEKCTGQCMISMADHQRNVDNQNTVTRKLGKDLKSIQGELTGILQIFNSINDTLKGLRNTIQKHGNNITDLNTSKGKIYLELDQLYDDLNKHKVDSKGHFDSISSFNSNLMTEMGECRLSREGLDKRLSKMEDVCGRLDTLSENLKTIKDGLSKHVSGLWTCVNDLNSTVISHSETISRIHNVHLENIQGRMNNLNSSIRNVLKEFQIFTEQDFTGPPGLPGPQGEKGSKGPPGPRGPLGKEGPQGRVGPVGPPGLRGEQGPPGKDANVPRLSFSAALTRPQASSGTIIFNKVFINERKAYNPKTGVFTAPVRGRYFFSAVLTGHKNVKIEAVLSKSNFGIARGDSAGYQPEGLEKPMAEARHTPGSLVIFNIVLPLQEGDTICIDLVTGKLAHSVEPLTIFSGMLLYEESEDRL

Target ORF information:

RefSeq Version	XM_005160756.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio elastin microfibril interfacer 1a (emilin1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160756.2

ORF Insert Sequence:

ATGGCACTAT ATTTTGTTTA CTTGTCCACT CTATTGGCGC TGATTTTGCT TGGAGATAAT 
TGGGCAGCGG GAACTTACGC TTCTCGATAC ACTCAACATG TGGATGAGAA TCAGTCTGGA 
GCAGCAGCAC AGAGTGGATC TAAGGTGACC AGCAGACATA GGAACTGGTG TGCCTACGTA 
GTGACGCGGA CTGTAAGCTG TGTTATGGAA GATGGAGTGG AAACTTATGT CAAACCTGAA 
TACCAGCGCT GTGCTTGGGG CCAATGTTCC CATGTGGTTT TGTATCGGAC TTACAGAAAA 
CCACGGTATA AGGTTGCCTA TAAAGTGGTA TCAGAGATGG AATGGAAGTG CTGTCATGGT 
TACTCAGGTG ATGACTGCAG TGACGGTTCC TCTGCCATAC ATGACAGCAG GGCAAGACCT 
ACTGGTGAAG AAGGGAGAAG TGACAGCGAC AGGATTAGAC AACTCGAGGA ACAAATACAA 
AGCTTGAACA AAAACCTTCA CAACCTCCAA AAGAAAATAT ATGAAGAATC TCAAAGGGAA 
GGCATCAGCG GTGGCAACAA TCTGGCAGAC GCTGCCCAGC CAGGGATGAA AGAGACCATT 
CACAGCATCC AGACCAAACT GGACATGCTG GACAACATGA CACGAGTGCA TGACAAGACC 
CTCACAAACA TCAACAACCA CCTTGTGGGC GGCAATGGAA TTGAGAATGA GTTGGACAGT 
CGCTATGGCA CACTTAAAGA GGAGATTCTA CGGGAGCTTG AACGGCGGGT GACGCTGTCT 
TGTTCGTCTT GCCAAACCGG AGTGGAGAGC ATACAAAGGC AACATCAGGA AGACAGAGAG 
AGGATCCGAG AACTGGAGAA GCACATCAGT GTGATGGAAC AGCACCACCA GCAAACCTTA 
GACTTGCTAT CACGCTCTCA GAGTTGCTGT GATTCCCTGG ACAGGAAGCT CAGTGCAATT 
GACAGAAAGG TAAGTTCGAC TGCAGAGACA TACGATATCC TTAGAGGACG CCTTGAGAAG 
GAGCTGAGAA GTAATGGCAA TGGAGGTCGT GGAAAAGCTA TGGAGGAAAA ACTGAATAAC 
CGCCTTCGAG ACCTGGAGCG GAGATTGAAT GGGACTGTGA GAAAAACTGA GCAAAAATGC 
TCCCATACAG AGACAAGTAT GAAGGAGTTT GTCCAGAGAG AGATCGGCCA GATAAAGAAC 
TCAGTCCTCG GTCGAAACGA TGATCATGGT TATAGGATAT CTACAGTTGA GATAGACATC 
CAAGACTTGA AGCGTTTTAT TAACGATCAT AAAAATAACC TGGAACGGTT AGGGAACAAA 
ACAAACGATC TTGACAGTGG GCTTAAGTCA GCGATTCATT TGTGCACAGA GACATGCGCT 
GCCAAAGGGA GTGAGACAGA AGATACTGTG AAAAGCCTGG AATGGAAGGT TGTTGCCAAT 
GAGGAGGACA TTAAGAAGTT TGACACCAAG CTAAAGGATA TAAGTGTGTC TGGTGACTCC 
TTATTGGACC GTGTAATAGA TCTAAGCAAT GACGTTCAAA AAATTAAGGA TCTGACAGGA 
CAAAATGGAG AACACTTTAA CCAAATCGTC ACCGATGTGG AGAATCTGGG ACGTGATTGT 
GACGTATGCA GCTCGATAGA TGGCGAACTA CAAAAAATAA GAAACGTCAC GAGCTACGCT 
TTCGACAAAT TCCAGGCAGA GCTTACAGTT TTGGGCAGAA AGGTATTTTC AGATGAGCAC 
GTTTGCTCAC AGGTGTGCTC AAACCTCCAG GAGGAAGTGG GCAAATTGAA AGAAGAGGTT 
GAAAAATGCA CAGGCCAATG CATGATCAGT ATGGCTGACC ATCAGAGAAA TGTAGACAAT 
CAAAATACTG TAACTCGCAA GCTTGGCAAA GACCTTAAAT CTATTCAAGG TGAGCTTACA 
GGGATTCTCC AAATATTCAA CTCCATCAAC GATACTCTAA AAGGCCTCAG GAACACCATA 
CAGAAGCATG GCAACAATAT TACTGATCTG AACACGTCTA AGGGCAAAAT CTACTTGGAG 
TTGGATCAGC TTTATGATGA TCTGAACAAG CACAAAGTGG ACAGCAAAGG ACATTTCGAC 
AGCATCAGTA GCTTTAACAG CAACTTGATG ACTGAAATGG GAGAATGCAG ACTTTCTCGA 
GAGGGCCTGG ATAAAAGGCT TTCAAAGATG GAAGATGTCT GCGGTAGACT GGATACTTTG 
TCAGAGAACC TTAAGACAAT TAAAGATGGC CTGAGCAAAC ACGTCTCTGG GCTTTGGACT 
TGTGTTAATG ATCTAAACTC CACGGTTATC TCTCACAGTG AGACCATTAG CCGCATTCAT 
AATGTTCACT TGGAGAACAT TCAAGGCCGG ATGAACAATC TTAACTCTTC CATACGGAAT 
GTTCTCAAGG AGTTCCAGAT CTTCACTGAA CAGGACTTTA CTGGACCTCC AGGTCTGCCA 
GGTCCTCAAG GAGAGAAAGG ATCTAAAGGA CCACCTGGGC CACGAGGACC TCTAGGAAAG 
GAAGGCCCAC AAGGGAGAGT GGGGCCGGTA GGACCCCCAG GGCTCAGAGG TGAACAAGGA 
CCTCCTGGAA AGGATGCCAA TGTTCCAAGA CTGTCGTTCT CAGCAGCCCT CACAAGACCC 
CAAGCCAGTT CAGGCACAAT TATTTTCAAC AAGGTCTTCA TCAATGAAAG AAAGGCCTAC 
AATCCAAAAA CAGGTGTGTT CACTGCTCCA GTGAGGGGGC GATACTTCTT CAGTGCAGTC 
TTGACAGGTC ACAAAAATGT CAAGATAGAG GCGGTTTTAT CTAAATCCAA TTTTGGCATT 
GCCCGTGGAG ATTCAGCAGG CTATCAGCCC GAGGGTCTTG AGAAGCCCAT GGCTGAAGCA 
CGGCACACTC CAGGTTCTCT GGTCATTTTT AATATCGTAC TTCCTCTGCA GGAGGGAGAT 
ACAATCTGCA TTGATTTGGT CACAGGTAAA CTTGCCCACT CCGTGGAGCC GCTCACCATC 
TTCAGTGGGA TGTTGCTCTA TGAGGAATCA GAGGACAGGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emilin1a ( XM_005160756.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160813.1 Danio rerio elastin microfibril interfacer 1a (emilin1a), transcript variant X1, mRNA.

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CloneID	ODa25031
Clone ID Related Accession (Same CDS sequence)	XM_005160756.2 , XM_005160756.3
Accession Version	XM_005160756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	EMILIN-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160756.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTAT ATTTTGTTTA CTTGTCCACT CTATTGGCGC TGATTTTGCT TGGAGATAAT 
TGGGCAGCGG GAACTTACGC TTCTCGATAC ACTCAACATG TGGATGAGAA TCAGTCTGGA 
GCAGCAGCAC AGAGTGGATC TAAGGTGACC AGCAGACATA GGAACTGGTG TGCCTACGTA 
GTGACGCGGA CTGTAAGCTG TGTTATGGAA GATGGAGTGG AAACTTATGT CAAACCTGAA 
TACCAGCGCT GTGCTTGGGG CCAATGTTCC CATGTGGTTT TGTATCGGAC TTACAGAAAA 
CCACGGTATA AGGTTGCCTA TAAAGTGGTA TCAGAGATGG AATGGAAGTG CTGTCATGGT 
TACTCAGGTG ATGACTGCAG TGACGGTTCC TCTGCCATAC ATGACAGCAG GGCAAGACCT 
ACTGGTGAAG AAGGGAGAAG TGACAGCGAC AGGATTAGAC AACTCGAGGA ACAAATACAA 
AGCTTGAACA AAAACCTTCA CAACCTCCAA AAGAAAATAT ATGAAGAATC TCAAAGGGAA 
GGCATCAGCG GTGGCAACAA TCTGGCAGAC GCTGCCCAGC CAGGGATGAA AGAGACCATT 
CACAGCATCC AGACCAAACT GGACATGCTG GACAACATGA CACGAGTGCA TGACAAGACC 
CTCACAAACA TCAACAACCA CCTTGTGGGC GGCAATGGAA TTGAGAATGA GTTGGACAGT 
CGCTATGGCA CACTTAAAGA GGAGATTCTA CGGGAGCTTG AACGGCGGGT GACGCTGTCT 
TGTTCGTCTT GCCAAACCGG AGTGGAGAGC ATACAAAGGC AACATCAGGA AGACAGAGAG 
AGGATCCGAG AACTGGAGAA GCACATCAGT GTGATGGAAC AGCACCACCA GCAAACCTTA 
GACTTGCTAT CACGCTCTCA GAGTTGCTGT GATTCCCTGG ACAGGAAGCT CAGTGCAATT 
GACAGAAAGG TAAGTTCGAC TGCAGAGACA TACGATATCC TTAGAGGACG CCTTGAGAAG 
GAGCTGAGAA GTAATGGCAA TGGAGGTCGT GGAAAAGCTA TGGAGGAAAA ACTGAATAAC 
CGCCTTCGAG ACCTGGAGCG GAGATTGAAT GGGACTGTGA GAAAAACTGA GCAAAAATGC 
TCCCATACAG AGACAAGTAT GAAGGAGTTT GTCCAGAGAG AGATCGGCCA GATAAAGAAC 
TCAGTCCTCG GTCGAAACGA TGATCATGGT TATAGGATAT CTACAGTTGA GATAGACATC 
CAAGACTTGA AGCGTTTTAT TAACGATCAT AAAAATAACC TGGAACGGTT AGGGAACAAA 
ACAAACGATC TTGACAGTGG GCTTAAGTCA GCGATTCATT TGTGCACAGA GACATGCGCT 
GCCAAAGGGA GTGAGACAGA AGATACTGTG AAAAGCCTGG AATGGAAGGT TGTTGCCAAT 
GAGGAGGACA TTAAGAAGTT TGACACCAAG CTAAAGGATA TAAGTGTGTC TGGTGACTCC 
TTATTGGACC GTGTAATAGA TCTAAGCAAT GACGTTCAAA AAATTAAGGA TCTGACAGGA 
CAAAATGGAG AACACTTTAA CCAAATCGTC ACCGATGTGG AGAATCTGGG ACGTGATTGT 
GACGTATGCA GCTCGATAGA TGGCGAACTA CAAAAAATAA GAAACGTCAC GAGCTACGCT 
TTCGACAAAT TCCAGGCAGA GCTTACAGTT TTGGGCAGAA AGGTATTTTC AGATGAGCAC 
GTTTGCTCAC AGGTGTGCTC AAACCTCCAG GAGGAAGTGG GCAAATTGAA AGAAGAGGTT 
GAAAAATGCA CAGGCCAATG CATGATCAGT ATGGCTGACC ATCAGAGAAA TGTAGACAAT 
CAAAATACTG TAACTCGCAA GCTTGGCAAA GACCTTAAAT CTATTCAAGG TGAGCTTACA 
GGGATTCTCC AAATATTCAA CTCCATCAAC GATACTCTAA AAGGCCTCAG GAACACCATA 
CAGAAGCATG GCAACAATAT TACTGATCTG AACACGTCTA AGGGCAAAAT CTACTTGGAG 
TTGGATCAGC TTTATGATGA TCTGAACAAG CACAAAGTGG ACAGCAAAGG ACATTTCGAC 
AGCATCAGTA GCTTTAACAG CAACTTGATG ACTGAAATGG GAGAATGCAG ACTTTCTCGA 
GAGGGCCTGG ATAAAAGGCT TTCAAAGATG GAAGATGTCT GCGGTAGACT GGATACTTTG 
TCAGAGAACC TTAAGACAAT TAAAGATGGC CTGAGCAAAC ACGTCTCTGG GCTTTGGACT 
TGTGTTAATG ATCTAAACTC CACGGTTATC TCTCACAGTG AGACCATTAG CCGCATTCAT 
AATGTTCACT TGGAGAACAT TCAAGGCCGG ATGAACAATC TTAACTCTTC CATACGGAAT 
GTTCTCAAGG AGTTCCAGAT CTTCACTGAA CAGGACTTTA CTGGACCTCC AGGTCTGCCA 
GGTCCTCAAG GAGAGAAAGG ATCTAAAGGA CCACCTGGGC CACGAGGACC TCTAGGAAAG 
GAAGGCCCAC AAGGGAGAGT GGGGCCGGTA GGACCCCCAG GGCTCAGAGG TGAACAAGGA 
CCTCCTGGAA AGGATGCCAA TGTTCCAAGA CTGTCGTTCT CAGCAGCCCT CACAAGACCC 
CAAGCCAGTT CAGGCACAAT TATTTTCAAC AAGGTCTTCA TCAATGAAAG AAAGGCCTAC 
AATCCAAAAA CAGGTGTGTT CACTGCTCCA GTGAGGGGGC GATACTTCTT CAGTGCAGTC 
TTGACAGGTC ACAAAAATGT CAAGATAGAG GCGGTTTTAT CTAAATCCAA TTTTGGCATT 
GCCCGTGGAG ATTCAGCAGG CTATCAGCCC GAGGGTCTTG AGAAGCCCAT GGCTGAAGCA 
CGGCACACTC CAGGTTCTCT GGTCATTTTT AATATCGTAC TTCCTCTGCA GGAGGGAGAT 
ACAATCTGCA TTGATTTGGT CACAGGTAAA CTTGCCCACT CCGTGGAGCC GCTCACCATC 
TTCAGTGGGA TGTTGCTCTA TGAGGAATCA GAGGACAGGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160813.1
CDS	341..3385
Translation	MALYFVYLSTLLALILLGDNWAAGTYASRYTQHVDENQSGAAAQSGSKVTSRHRNWCAYVVTRTVSCVMEDGVETYVKPEYQRCAWGQCSHVVLYRTYRKPRYKVAYKVVSEMEWKCCHGYSGDDCSDGSSAIHDSRARPTGEEGRSDSDRIRQLEEQIQSLNKNLHNLQKKIYEESQREGISGGNNLADAAQPGMKETIHSIQTKLDMLDNMTRVHDKTLTNINNHLVGGNGIENELDSRYGTLKEEILRELERRVTLSCSSCQTGVESIQRQHQEDRERIRELEKHISVMEQHHQQTLDLLSRSQSCCDSLDRKLSAIDRKVSSTAETYDILRGRLEKELRSNGNGGRGKAMEEKLNNRLRDLERRLNGTVRKTEQKCSHTETSMKEFVQREIGQIKNSVLGRNDDHGYRISTVEIDIQDLKRFINDHKNNLERLGNKTNDLDSGLKSAIHLCTETCAAKGSETEDTVKSLEWKVVANEEDIKKFDTKLKDISVSGDSLLDRVIDLSNDVQKIKDLTGQNGEHFNQIVTDVENLGRDCDVCSSIDGELQKIRNVTSYAFDKFQAELTVLGRKVFSDEHVCSQVCSNLQEEVGKLKEEVEKCTGQCMISMADHQRNVDNQNTVTRKLGKDLKSIQGELTGILQIFNSINDTLKGLRNTIQKHGNNITDLNTSKGKIYLELDQLYDDLNKHKVDSKGHFDSISSFNSNLMTEMGECRLSREGLDKRLSKMEDVCGRLDTLSENLKTIKDGLSKHVSGLWTCVNDLNSTVISHSETISRIHNVHLENIQGRMNNLNSSIRNVLKEFQIFTEQDFTGPPGLPGPQGEKGSKGPPGPRGPLGKEGPQGRVGPVGPPGLRGEQGPPGKDANVPRLSFSAALTRPQASSGTIIFNKVFINERKAYNPKTGVFTAPVRGRYFFSAVLTGHKNVKIEAVLSKSNFGIARGDSAGYQPEGLEKPMAEARHTPGSLVIFNIVLPLQEGDTICIDLVTGKLAHSVEPLTIFSGMLLYEESEDRL

Target ORF information:

RefSeq Version	XM_005160756.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio elastin microfibril interfacer 1a (emilin1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160756.3

ORF Insert Sequence:

ATGGCACTAT ATTTTGTTTA CTTGTCCACT CTATTGGCGC TGATTTTGCT TGGAGATAAT 
TGGGCAGCGG GAACTTACGC TTCTCGATAC ACTCAACATG TGGATGAGAA TCAGTCTGGA 
GCAGCAGCAC AGAGTGGATC TAAGGTGACC AGCAGACATA GGAACTGGTG TGCCTACGTA 
GTGACGCGGA CTGTAAGCTG TGTTATGGAA GATGGAGTGG AAACTTATGT CAAACCTGAA 
TACCAGCGCT GTGCTTGGGG CCAATGTTCC CATGTGGTTT TGTATCGGAC TTACAGAAAA 
CCACGGTATA AGGTTGCCTA TAAAGTGGTA TCAGAGATGG AATGGAAGTG CTGTCATGGT 
TACTCAGGTG ATGACTGCAG TGACGGTTCC TCTGCCATAC ATGACAGCAG GGCAAGACCT 
ACTGGTGAAG AAGGGAGAAG TGACAGCGAC AGGATTAGAC AACTCGAGGA ACAAATACAA 
AGCTTGAACA AAAACCTTCA CAACCTCCAA AAGAAAATAT ATGAAGAATC TCAAAGGGAA 
GGCATCAGCG GTGGCAACAA TCTGGCAGAC GCTGCCCAGC CAGGGATGAA AGAGACCATT 
CACAGCATCC AGACCAAACT GGACATGCTG GACAACATGA CACGAGTGCA TGACAAGACC 
CTCACAAACA TCAACAACCA CCTTGTGGGC GGCAATGGAA TTGAGAATGA GTTGGACAGT 
CGCTATGGCA CACTTAAAGA GGAGATTCTA CGGGAGCTTG AACGGCGGGT GACGCTGTCT 
TGTTCGTCTT GCCAAACCGG AGTGGAGAGC ATACAAAGGC AACATCAGGA AGACAGAGAG 
AGGATCCGAG AACTGGAGAA GCACATCAGT GTGATGGAAC AGCACCACCA GCAAACCTTA 
GACTTGCTAT CACGCTCTCA GAGTTGCTGT GATTCCCTGG ACAGGAAGCT CAGTGCAATT 
GACAGAAAGG TAAGTTCGAC TGCAGAGACA TACGATATCC TTAGAGGACG CCTTGAGAAG 
GAGCTGAGAA GTAATGGCAA TGGAGGTCGT GGAAAAGCTA TGGAGGAAAA ACTGAATAAC 
CGCCTTCGAG ACCTGGAGCG GAGATTGAAT GGGACTGTGA GAAAAACTGA GCAAAAATGC 
TCCCATACAG AGACAAGTAT GAAGGAGTTT GTCCAGAGAG AGATCGGCCA GATAAAGAAC 
TCAGTCCTCG GTCGAAACGA TGATCATGGT TATAGGATAT CTACAGTTGA GATAGACATC 
CAAGACTTGA AGCGTTTTAT TAACGATCAT AAAAATAACC TGGAACGGTT AGGGAACAAA 
ACAAACGATC TTGACAGTGG GCTTAAGTCA GCGATTCATT TGTGCACAGA GACATGCGCT 
GCCAAAGGGA GTGAGACAGA AGATACTGTG AAAAGCCTGG AATGGAAGGT TGTTGCCAAT 
GAGGAGGACA TTAAGAAGTT TGACACCAAG CTAAAGGATA TAAGTGTGTC TGGTGACTCC 
TTATTGGACC GTGTAATAGA TCTAAGCAAT GACGTTCAAA AAATTAAGGA TCTGACAGGA 
CAAAATGGAG AACACTTTAA CCAAATCGTC ACCGATGTGG AGAATCTGGG ACGTGATTGT 
GACGTATGCA GCTCGATAGA TGGCGAACTA CAAAAAATAA GAAACGTCAC GAGCTACGCT 
TTCGACAAAT TCCAGGCAGA GCTTACAGTT TTGGGCAGAA AGGTATTTTC AGATGAGCAC 
GTTTGCTCAC AGGTGTGCTC AAACCTCCAG GAGGAAGTGG GCAAATTGAA AGAAGAGGTT 
GAAAAATGCA CAGGCCAATG CATGATCAGT ATGGCTGACC ATCAGAGAAA TGTAGACAAT 
CAAAATACTG TAACTCGCAA GCTTGGCAAA GACCTTAAAT CTATTCAAGG TGAGCTTACA 
GGGATTCTCC AAATATTCAA CTCCATCAAC GATACTCTAA AAGGCCTCAG GAACACCATA 
CAGAAGCATG GCAACAATAT TACTGATCTG AACACGTCTA AGGGCAAAAT CTACTTGGAG 
TTGGATCAGC TTTATGATGA TCTGAACAAG CACAAAGTGG ACAGCAAAGG ACATTTCGAC 
AGCATCAGTA GCTTTAACAG CAACTTGATG ACTGAAATGG GAGAATGCAG ACTTTCTCGA 
GAGGGCCTGG ATAAAAGGCT TTCAAAGATG GAAGATGTCT GCGGTAGACT GGATACTTTG 
TCAGAGAACC TTAAGACAAT TAAAGATGGC CTGAGCAAAC ACGTCTCTGG GCTTTGGACT 
TGTGTTAATG ATCTAAACTC CACGGTTATC TCTCACAGTG AGACCATTAG CCGCATTCAT 
AATGTTCACT TGGAGAACAT TCAAGGCCGG ATGAACAATC TTAACTCTTC CATACGGAAT 
GTTCTCAAGG AGTTCCAGAT CTTCACTGAA CAGGACTTTA CTGGACCTCC AGGTCTGCCA 
GGTCCTCAAG GAGAGAAAGG ATCTAAAGGA CCACCTGGGC CACGAGGACC TCTAGGAAAG 
GAAGGCCCAC AAGGGAGAGT GGGGCCGGTA GGACCCCCAG GGCTCAGAGG TGAACAAGGA 
CCTCCTGGAA AGGATGCCAA TGTTCCAAGA CTGTCGTTCT CAGCAGCCCT CACAAGACCC 
CAAGCCAGTT CAGGCACAAT TATTTTCAAC AAGGTCTTCA TCAATGAAAG AAAGGCCTAC 
AATCCAAAAA CAGGTGTGTT CACTGCTCCA GTGAGGGGGC GATACTTCTT CAGTGCAGTC 
TTGACAGGTC ACAAAAATGT CAAGATAGAG GCGGTTTTAT CTAAATCCAA TTTTGGCATT 
GCCCGTGGAG ATTCAGCAGG CTATCAGCCC GAGGGTCTTG AGAAGCCCAT GGCTGAAGCA 
CGGCACACTC CAGGTTCTCT GGTCATTTTT AATATCGTAC TTCCTCTGCA GGAGGGAGAT 
ACAATCTGCA TTGATTTGGT CACAGGTAAA CTTGCCCACT CCGTGGAGCC GCTCACCATC 
TTCAGTGGGA TGTTGCTCTA TGAGGAATCA GAGGACAGGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Genetic compensation induced by deleterious mutations but not gene knockdowns.
Nature524(7564)230-3(2015 Aug)
Rossi A,Kontarakis Z,Gerri C,Nolte H,Hölper S,Krüger M,Stainier DY

Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators.
Developmental cell20(5)597-609(2011 May)
Tijssen MR,Cvejic A,Joshi A,Hannah RL,Ferreira R,Forrai A,Bellissimo DC,Oram SH,Smethurst PA,Wilson NK,Wang X,Ottersbach K,Stemple DL,Green AR,Ouwehand WH,Göttgens B

Characterization of C1q in teleosts: insight into the molecular and functional evolution of C1q family and classical pathway.
The Journal of biological chemistry285(37)28777-86(2010 Sep)
Hu YL,Pan XM,Xiang LX,Shao JZ

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emilin1a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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