Emilin1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Emilin1
Entrez Gene ID	100952
Full Name	elastin microfibril interfacer 1
Synonyms	5830419M17Rik,AW229038,BB105748,EMILIN-1,gp115
General protein information	Preferred Names elastin microfibril interfacer 1 Names EMILIN-1 elastin microfibril interface located protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006503649.3	XP_006503712.1	EMILIN-1 isoform X2
XM_006503649.4	XP_006503712.1	EMILIN-1 isoform X2
NM_133918.2	NP_598679.1	EMILIN-1 precursor
XM_006503648.2	XP_006503711.1	EMILIN-1 isoform X1
XM_006503648.3	XP_006503711.1	EMILIN-1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	EMILIN1	NP_001179363.1
Mus musculus (house mouse)	Emilin1	NP_598679.1
Pan troglodytes (chimpanzee)	EMILIN1	XP_515351.2
Macaca mulatta (Rhesus monkey)	EMILIN1	XP_001089257.1
Rattus norvegicus (Norway rat)	Emilin1	NP_001100180.1
Homo sapiens (human)	EMILIN1	NP_008977.1
Canis lupus familiaris (dog)	EMILIN1	XP_003432201.1
Danio rerio (zebrafish)	emilin1a	NP_001025378.1
Xenopus tropicalis (tropical clawed frog)	emilin1	NP_001090870.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of EMILIN-1 Enhances Arteriolar Myogenic Tone Through TGF-β (Transforming Growth Factor-β)-Dependent Transactivation of EGFR (Epidermal Growth Factor Receptor) and Is Relevant for Hypertension in Mice and Humans.
Carnevale D, Facchinello N, Iodice D, Bizzotto D, Perrotta M, De Stefani D, Pallante F, Carnevale L, Ricciardi F, Cifelli G, Da Ros F, Casaburo M, Fardella S, Bonaldo P, Innocenzi G, Rizzuto R, Braghetta P, Lembo G, Bressan GM
Arteriosclerosis, thrombosis, and vascular biology38(10)2484-2497(2018 10)

Lhx8 ablation leads to massive autophagy of mouse oocytes associated with DNA damage.
D'Ignazio L, Michel M, Beyer M, Thompson K, Forabosco A, Schlessinger D, Pelosi E
Biology of reproduction98(4)532-542(2018 04)

Altered feto-placental vascularization, feto-placental malperfusion and fetal growth restriction in mice with
Lacko LA, Hurtado R, Hinds S, Poulos MG, Butler JM, Stuhlmann H
Development (Cambridge, England)144(13)2469-2479(2017 07)

Fibulin-4 deposition requires EMILIN-1 in the extracellular matrix of osteoblasts.
Schiavinato A, Keene DR, Imhof T, Doliana R, Sasaki T, Sengle G
Scientific reports7(1)5526(2017 07)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Emilin1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Emilin1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34885	XM_006503648.3 Latest version!	Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu34886	XM_006503649.4 Latest version!	Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu10358	NM_133918.2 Latest version!	Mus musculus elastin microfibril interfacer 1 (Emilin1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu34886	XM_006503649.3	Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu34885	XM_006503648.2	Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Emilin1 ( XM_006503648.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503711.1 Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34885
Clone ID Related Accession (Same CDS sequence)	XM_006503648.2 , XM_006503648.3
Accession Version	XM_006503648.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	EMILIN-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006503648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGA GCTTTCTTCG CCCTCGCTAT CGGGTGGCTT ACAAGACAGT GACAGACATG 
GAGTGGAGGT GCTGTCAGGG TTATGGGGGC GATGACTGTG GAGAGGGCCC TGCTTCCGTT 
CTGGGCCCAG CACCCTCCAC ACCACTTCCC CGGCCCAGGC CTGTGCGCCC CAACCTGTCT 
GGCTCCAGTG CCGGCAGCCA TCTCAGTGGA CTGGGAGGAG AAGGTCCTGT TGAGTCAGAG 
AAGGTGCAGC AGCTAGAGCG GCAGGTGAAG AGTCTGACCA AAGAGCTGCA AGGCCTTCGG 
GGTGTCCTGC AGGGGATGAA TGGGCGCCTA GCAGAAGATG TACAGCGAGC TGTGGACACA 
GTCTTTAATG GAAGGCAGCA GCCAGCAGAT GCAGCTGCCC GCCCGGGCGT GCATGAAACC 
CTCAGTGAGA TCCAGCAGCA GCTGCAGCTC CTGGACAACC GTGTCTCCAC TCATGACCAG 
GAGCTGGGCC ACCTTAACAA CCATCATAAT GGAGGCCCTG GTGGAGGTGG CAGGGCCTCA 
GGCCCTGTCC CAGTTCCTTC TGGCCCCAGT GAAGAACTGT TAAGGCAGCT GGAACGGCAG 
CTGCAAGAGT CTTGCTCAGT GTGCCTGACG GGGCTGGATG GCTTCCGCCA GCAGCAGCAA 
GAGGATAGGG AGCGGCTGCG AACCCTGGAG AAGCTAATGT CCTCTATGGA GGAGCGGCAG 
CAACAGCTTG TGGGACCTGC CATGGCCAGG AGACCCCCTC AGGAATGCTG CCCCCCAGAG 
CTGGGTCGAC GAGTGTCTGA GCTGGAGCGG AGGCTAGATG TAGTGACTGG CTCACTGACA 
GTGCTAAGTG GACGCAGAGG TTCTGAGCTT GGAGGAGCAG CTGGGCAGGG GGGCCACCCT 
CCAGGCTACA CCAGCTTGGC CTCCCGCCTT TCTCGCCTGG AGGACCGCTT CAACTCTACC 
CTAGGTCCCT CAGAGGAGCA AGAGAAGAAC TGGCCTGGAG GACCAGGGAG GCTGGGCCAC 
TGGTTGCCTG CTGCTCCAGG ACGGCTAGAA AAGCTGGAGG GACTACTAGC CAATGTGAGC 
AGGGAGCTGG GTGGCCGCAT GGATCTGCTG GAAGAGCAGG TGGCAGGGGC TGTTCGGACT 
TGTGGGCAGA TTTGCTCTGG GGCACCCGGG GAACAGGATT CTCGGGTCAA TGAGATCCTC 
AGTGCCTTGG AACGCAGGGT GCTGGACAGC GAGGGCCGGT TACAGCTAGT GGGCTCTGGC 
TTGCATGAAG CAGAGGCAGC AGGGGAGGCT CAGCAGGCCG TGTTGGAGGG ACTGCAAGGG 
CTCCTGAGCC GGCTTCGGGA GCGCATGGAT GCACAGGAGG AGACTGCAGC AGAAATCTTA 
CTGCGCCTCA ATCTTACCGC AGCCCAGCTA AGCCAGCTGG AGGGTCTGCT GCAAGCCCGT 
GGGGATGAGG GTTGTGGCGC CTGTGGTGGT GTCCAGGAGG AGCTGGGCCG CCTTCGGGAT 
GGTGTGGAAC GTTGCTCCTG CCCATTGTTA CCTCCACGGG GCCCTGGAGC TGGCCCAGGG 
GTTGGGGGAC CAAGCCGTGG GCCTCTGGAT GGTTTCAGTG TGTTTGGGGG CAGTTCAGGC 
TCAGCCCTCC AGGCCCTTCA AGGAGAACTC TCTGAGGTTA TTCTCACCTT CAGCTCCCTG 
AACGACTCAC TCCACGAGCT CCAGACCACT GTGGAGGGTC AGGGTGCCGA TCTGGCTGAC 
CTGGGGGCCA CCAAGGACAG CATCATCTCT GAAATCAACA GACTACAGCA GGAGGCCACG 
GAGCACGTCA CAGAGAGCGA GGAGCGCTTC CGAGGCCTGG AGGAGGGCCA GGCACAGGCT 
GGCCAATGCC CTAGCTTAGA GGGGCGATTA GGCCGCCTTG AGGGAGTCTG CGAACGACTG 
GACACCGTGG CAGGGGGACT ACAAGGCCTA CGTGAAGGCC TCTCCAGACA CGTGGCTGGG 
CTCTGGGCTG CAGTACGGGA AAGCAACAGC ACCAGCCTGA CACAGGCCGC CCTGCTGGAG 
AAGCTGCTGG GGGGCCAGGC AGGCCTGGGC AGGCGGCTTG GTGCCCTCAA CAATTCCCTG 
CTGCTCCTGG AAGACCGTCT GCAGCAACTT AGCCTAAAGG ACTTCACTGG ACCTTCAGGC 
AAGGCTGGGC CCCCAGGGCC TCCCGGGCTA CAAGGGCCTT CAGGCCCTGC AGGACCTCCA 
GGACCTCCTG GCAAAGACGG ACAACAGGGG GCCATTGGCC CACCAGGTCC TCAAGGGGAG 
CAGGGAGCGG AGGGAGCACC AGCAGCCCCT GTGCCTAGGG TAGCATTTTC AGCTGCCCTG 
AGTTTGCCAC GGTCAGAACC TGGCACAGTC CCCTTCGACA GAGTCTTGCT CAATGATGGA 
GGCTACTATG ACCCAGAGAC AGGTGTATTC ACTGCACCAC TGGCTGGACG CTATTTGCTG 
AGCGCTGTAC TTACTGGGCA CCGGCATGAG AAAGTGGAAG CAGTATTGTC ACGCTCCAAC 
CTGGGCGTGG CCCGCATAGA CTCGGGAGGC TATGAACCCG AGGGACTGGA GAATAAGCCT 
GTGGCCGAAA GTCAGCCCAG CCCAGGCGCT CTGGGCGTCT TCAGCCTCAT TCTGCCACTG 
CAGGTCGGAG ACACTGTCTG CATCGACCTG GTCATGGGGC AGCTGGCACA CTCCGAGGAG 
CCGCTCACCA TCTTCAGCGG AGCCCTGCTC TACGAGGACA CAGAGCTTGA ACAGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503711.1
CDS	806..3565
Translation	MYRSFLRPRYRVAYKTVTDMEWRCCQGYGGDDCGEGPASVLGPAPSTPLPRPRPVRPNLSGSSAGSHLSGLGGEGPVESEKVQQLERQVKSLTKELQGLRGVLQGMNGRLAEDVQRAVDTVFNGRQQPADAAARPGVHETLSEIQQQLQLLDNRVSTHDQELGHLNNHHNGGPGGGGRASGPVPVPSGPSEELLRQLERQLQESCSVCLTGLDGFRQQQQEDRERLRTLEKLMSSMEERQQQLVGPAMARRPPQECCPPELGRRVSELERRLDVVTGSLTVLSGRRGSELGGAAGQGGHPPGYTSLASRLSRLEDRFNSTLGPSEEQEKNWPGGPGRLGHWLPAAPGRLEKLEGLLANVSRELGGRMDLLEEQVAGAVRTCGQICSGAPGEQDSRVNEILSALERRVLDSEGRLQLVGSGLHEAEAAGEAQQAVLEGLQGLLSRLRERMDAQEETAAEILLRLNLTAAQLSQLEGLLQARGDEGCGACGGVQEELGRLRDGVERCSCPLLPPRGPGAGPGVGGPSRGPLDGFSVFGGSSGSALQALQGELSEVILTFSSLNDSLHELQTTVEGQGADLADLGATKDSIISEINRLQQEATEHVTESEERFRGLEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGLQGLREGLSRHVAGLWAAVRESNSTSLTQAALLEKLLGGQAGLGRRLGALNNSLLLLEDRLQQLSLKDFTGPSGKAGPPGPPGLQGPSGPAGPPGPPGKDGQQGAIGPPGPQGEQGAEGAPAAPVPRVAFSAALSLPRSEPGTVPFDRVLLNDGGYYDPETGVFTAPLAGRYLLSAVLTGHRHEKVEAVLSRSNLGVARIDSGGYEPEGLENKPVAESQPSPGALGVFSLILPLQVGDTVCIDLVMGQLAHSEEPLTIFSGALLYEDTELEQV

Target ORF information:

RefSeq Version	XM_006503648.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503648.2

ORF Insert Sequence:

ATGTACCGGA GCTTTCTTCG CCCTCGCTAT CGGGTGGCTT ACAAGACAGT GACAGACATG 
GAGTGGAGGT GCTGTCAGGG TTATGGGGGC GATGACTGTG GAGAGGGCCC TGCTTCCGTT 
CTGGGCCCAG CACCCTCCAC ACCACTTCCC CGGCCCAGGC CTGTGCGCCC CAACCTGTCT 
GGCTCCAGTG CCGGCAGCCA TCTCAGTGGA CTGGGAGGAG AAGGTCCTGT TGAGTCAGAG 
AAGGTGCAGC AGCTAGAGCG GCAGGTGAAG AGTCTGACCA AAGAGCTGCA AGGCCTTCGG 
GGTGTCCTGC AGGGGATGAA TGGGCGCCTA GCAGAAGATG TACAGCGAGC TGTGGACACA 
GTCTTTAATG GAAGGCAGCA GCCAGCAGAT GCAGCTGCCC GCCCGGGCGT GCATGAAACC 
CTCAGTGAGA TCCAGCAGCA GCTGCAGCTC CTGGACAACC GTGTCTCCAC TCATGACCAG 
GAGCTGGGCC ACCTTAACAA CCATCATAAT GGAGGCCCTG GTGGAGGTGG CAGGGCCTCA 
GGCCCTGTCC CAGTTCCTTC TGGCCCCAGT GAAGAACTGT TAAGGCAGCT GGAACGGCAG 
CTGCAAGAGT CTTGCTCAGT GTGCCTGACG GGGCTGGATG GCTTCCGCCA GCAGCAGCAA 
GAGGATAGGG AGCGGCTGCG AACCCTGGAG AAGCTAATGT CCTCTATGGA GGAGCGGCAG 
CAACAGCTTG TGGGACCTGC CATGGCCAGG AGACCCCCTC AGGAATGCTG CCCCCCAGAG 
CTGGGTCGAC GAGTGTCTGA GCTGGAGCGG AGGCTAGATG TAGTGACTGG CTCACTGACA 
GTGCTAAGTG GACGCAGAGG TTCTGAGCTT GGAGGAGCAG CTGGGCAGGG GGGCCACCCT 
CCAGGCTACA CCAGCTTGGC CTCCCGCCTT TCTCGCCTGG AGGACCGCTT CAACTCTACC 
CTAGGTCCCT CAGAGGAGCA AGAGAAGAAC TGGCCTGGAG GACCAGGGAG GCTGGGCCAC 
TGGTTGCCTG CTGCTCCAGG ACGGCTAGAA AAGCTGGAGG GACTACTAGC CAATGTGAGC 
AGGGAGCTGG GTGGCCGCAT GGATCTGCTG GAAGAGCAGG TGGCAGGGGC TGTTCGGACT 
TGTGGGCAGA TTTGCTCTGG GGCACCCGGG GAACAGGATT CTCGGGTCAA TGAGATCCTC 
AGTGCCTTGG AACGCAGGGT GCTGGACAGC GAGGGCCGGT TACAGCTAGT GGGCTCTGGC 
TTGCATGAAG CAGAGGCAGC AGGGGAGGCT CAGCAGGCCG TGTTGGAGGG ACTGCAAGGG 
CTCCTGAGCC GGCTTCGGGA GCGCATGGAT GCACAGGAGG AGACTGCAGC AGAAATCTTA 
CTGCGCCTCA ATCTTACCGC AGCCCAGCTA AGCCAGCTGG AGGGTCTGCT GCAAGCCCGT 
GGGGATGAGG GTTGTGGCGC CTGTGGTGGT GTCCAGGAGG AGCTGGGCCG CCTTCGGGAT 
GGTGTGGAAC GTTGCTCCTG CCCATTGTTA CCTCCACGGG GCCCTGGAGC TGGCCCAGGG 
GTTGGGGGAC CAAGCCGTGG GCCTCTGGAT GGTTTCAGTG TGTTTGGGGG CAGTTCAGGC 
TCAGCCCTCC AGGCCCTTCA AGGAGAACTC TCTGAGGTTA TTCTCACCTT CAGCTCCCTG 
AACGACTCAC TCCACGAGCT CCAGACCACT GTGGAGGGTC AGGGTGCCGA TCTGGCTGAC 
CTGGGGGCCA CCAAGGACAG CATCATCTCT GAAATCAACA GACTACAGCA GGAGGCCACG 
GAGCACGTCA CAGAGAGCGA GGAGCGCTTC CGAGGCCTGG AGGAGGGCCA GGCACAGGCT 
GGCCAATGCC CTAGCTTAGA GGGGCGATTA GGCCGCCTTG AGGGAGTCTG CGAACGACTG 
GACACCGTGG CAGGGGGACT ACAAGGCCTA CGTGAAGGCC TCTCCAGACA CGTGGCTGGG 
CTCTGGGCTG CAGTACGGGA AAGCAACAGC ACCAGCCTGA CACAGGCCGC CCTGCTGGAG 
AAGCTGCTGG GGGGCCAGGC AGGCCTGGGC AGGCGGCTTG GTGCCCTCAA CAATTCCCTG 
CTGCTCCTGG AAGACCGTCT GCAGCAACTT AGCCTAAAGG ACTTCACTGG ACCTTCAGGC 
AAGGCTGGGC CCCCAGGGCC TCCCGGGCTA CAAGGGCCTT CAGGCCCTGC AGGACCTCCA 
GGACCTCCTG GCAAAGACGG ACAACAGGGG GCCATTGGCC CACCAGGTCC TCAAGGGGAG 
CAGGGAGCGG AGGGAGCACC AGCAGCCCCT GTGCCTAGGG TAGCATTTTC AGCTGCCCTG 
AGTTTGCCAC GGTCAGAACC TGGCACAGTC CCCTTCGACA GAGTCTTGCT CAATGATGGA 
GGCTACTATG ACCCAGAGAC AGGTGTATTC ACTGCACCAC TGGCTGGACG CTATTTGCTG 
AGCGCTGTAC TTACTGGGCA CCGGCATGAG AAAGTGGAAG CAGTATTGTC ACGCTCCAAC 
CTGGGCGTGG CCCGCATAGA CTCGGGAGGC TATGAACCCG AGGGACTGGA GAATAAGCCT 
GTGGCCGAAA GTCAGCCCAG CCCAGGCGCT CTGGGCGTCT TCAGCCTCAT TCTGCCACTG 
CAGGTCGGAG ACACTGTCTG CATCGACCTG GTCATGGGGC AGCTGGCACA CTCCGAGGAG 
CCGCTCACCA TCTTCAGCGG AGCCCTGCTC TACGAGGACA CAGAGCTTGA ACAGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin1 ( XM_006503648.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503711.1 Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34885
Clone ID Related Accession (Same CDS sequence)	XM_006503648.2 , XM_006503648.3
Accession Version	XM_006503648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	EMILIN-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGA GCTTTCTTCG CCCTCGCTAT CGGGTGGCTT ACAAGACAGT GACAGACATG 
GAGTGGAGGT GCTGTCAGGG TTATGGGGGC GATGACTGTG GAGAGGGCCC TGCTTCCGTT 
CTGGGCCCAG CACCCTCCAC ACCACTTCCC CGGCCCAGGC CTGTGCGCCC CAACCTGTCT 
GGCTCCAGTG CCGGCAGCCA TCTCAGTGGA CTGGGAGGAG AAGGTCCTGT TGAGTCAGAG 
AAGGTGCAGC AGCTAGAGCG GCAGGTGAAG AGTCTGACCA AAGAGCTGCA AGGCCTTCGG 
GGTGTCCTGC AGGGGATGAA TGGGCGCCTA GCAGAAGATG TACAGCGAGC TGTGGACACA 
GTCTTTAATG GAAGGCAGCA GCCAGCAGAT GCAGCTGCCC GCCCGGGCGT GCATGAAACC 
CTCAGTGAGA TCCAGCAGCA GCTGCAGCTC CTGGACAACC GTGTCTCCAC TCATGACCAG 
GAGCTGGGCC ACCTTAACAA CCATCATAAT GGAGGCCCTG GTGGAGGTGG CAGGGCCTCA 
GGCCCTGTCC CAGTTCCTTC TGGCCCCAGT GAAGAACTGT TAAGGCAGCT GGAACGGCAG 
CTGCAAGAGT CTTGCTCAGT GTGCCTGACG GGGCTGGATG GCTTCCGCCA GCAGCAGCAA 
GAGGATAGGG AGCGGCTGCG AACCCTGGAG AAGCTAATGT CCTCTATGGA GGAGCGGCAG 
CAACAGCTTG TGGGACCTGC CATGGCCAGG AGACCCCCTC AGGAATGCTG CCCCCCAGAG 
CTGGGTCGAC GAGTGTCTGA GCTGGAGCGG AGGCTAGATG TAGTGACTGG CTCACTGACA 
GTGCTAAGTG GACGCAGAGG TTCTGAGCTT GGAGGAGCAG CTGGGCAGGG GGGCCACCCT 
CCAGGCTACA CCAGCTTGGC CTCCCGCCTT TCTCGCCTGG AGGACCGCTT CAACTCTACC 
CTAGGTCCCT CAGAGGAGCA AGAGAAGAAC TGGCCTGGAG GACCAGGGAG GCTGGGCCAC 
TGGTTGCCTG CTGCTCCAGG ACGGCTAGAA AAGCTGGAGG GACTACTAGC CAATGTGAGC 
AGGGAGCTGG GTGGCCGCAT GGATCTGCTG GAAGAGCAGG TGGCAGGGGC TGTTCGGACT 
TGTGGGCAGA TTTGCTCTGG GGCACCCGGG GAACAGGATT CTCGGGTCAA TGAGATCCTC 
AGTGCCTTGG AACGCAGGGT GCTGGACAGC GAGGGCCGGT TACAGCTAGT GGGCTCTGGC 
TTGCATGAAG CAGAGGCAGC AGGGGAGGCT CAGCAGGCCG TGTTGGAGGG ACTGCAAGGG 
CTCCTGAGCC GGCTTCGGGA GCGCATGGAT GCACAGGAGG AGACTGCAGC AGAAATCTTA 
CTGCGCCTCA ATCTTACCGC AGCCCAGCTA AGCCAGCTGG AGGGTCTGCT GCAAGCCCGT 
GGGGATGAGG GTTGTGGCGC CTGTGGTGGT GTCCAGGAGG AGCTGGGCCG CCTTCGGGAT 
GGTGTGGAAC GTTGCTCCTG CCCATTGTTA CCTCCACGGG GCCCTGGAGC TGGCCCAGGG 
GTTGGGGGAC CAAGCCGTGG GCCTCTGGAT GGTTTCAGTG TGTTTGGGGG CAGTTCAGGC 
TCAGCCCTCC AGGCCCTTCA AGGAGAACTC TCTGAGGTTA TTCTCACCTT CAGCTCCCTG 
AACGACTCAC TCCACGAGCT CCAGACCACT GTGGAGGGTC AGGGTGCCGA TCTGGCTGAC 
CTGGGGGCCA CCAAGGACAG CATCATCTCT GAAATCAACA GACTACAGCA GGAGGCCACG 
GAGCACGTCA CAGAGAGCGA GGAGCGCTTC CGAGGCCTGG AGGAGGGCCA GGCACAGGCT 
GGCCAATGCC CTAGCTTAGA GGGGCGATTA GGCCGCCTTG AGGGAGTCTG CGAACGACTG 
GACACCGTGG CAGGGGGACT ACAAGGCCTA CGTGAAGGCC TCTCCAGACA CGTGGCTGGG 
CTCTGGGCTG CAGTACGGGA AAGCAACAGC ACCAGCCTGA CACAGGCCGC CCTGCTGGAG 
AAGCTGCTGG GGGGCCAGGC AGGCCTGGGC AGGCGGCTTG GTGCCCTCAA CAATTCCCTG 
CTGCTCCTGG AAGACCGTCT GCAGCAACTT AGCCTAAAGG ACTTCACTGG ACCTTCAGGC 
AAGGCTGGGC CCCCAGGGCC TCCCGGGCTA CAAGGGCCTT CAGGCCCTGC AGGACCTCCA 
GGACCTCCTG GCAAAGACGG ACAACAGGGG GCCATTGGCC CACCAGGTCC TCAAGGGGAG 
CAGGGAGCGG AGGGAGCACC AGCAGCCCCT GTGCCTAGGG TAGCATTTTC AGCTGCCCTG 
AGTTTGCCAC GGTCAGAACC TGGCACAGTC CCCTTCGACA GAGTCTTGCT CAATGATGGA 
GGCTACTATG ACCCAGAGAC AGGTGTATTC ACTGCACCAC TGGCTGGACG CTATTTGCTG 
AGCGCTGTAC TTACTGGGCA CCGGCATGAG AAAGTGGAAG CAGTATTGTC ACGCTCCAAC 
CTGGGCGTGG CCCGCATAGA CTCGGGAGGC TATGAACCCG AGGGACTGGA GAATAAGCCT 
GTGGCCGAAA GTCAGCCCAG CCCAGGCGCT CTGGGCGTCT TCAGCCTCAT TCTGCCACTG 
CAGGTCGGAG ACACTGTCTG CATCGACCTG GTCATGGGGC AGCTGGCACA CTCCGAGGAG 
CCGCTCACCA TCTTCAGCGG AGCCCTGCTC TACGAGGACA CAGAGCTTGA ACAGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503711.1
CDS	801..3560
Translation	MYRSFLRPRYRVAYKTVTDMEWRCCQGYGGDDCGEGPASVLGPAPSTPLPRPRPVRPNLSGSSAGSHLSGLGGEGPVESEKVQQLERQVKSLTKELQGLRGVLQGMNGRLAEDVQRAVDTVFNGRQQPADAAARPGVHETLSEIQQQLQLLDNRVSTHDQELGHLNNHHNGGPGGGGRASGPVPVPSGPSEELLRQLERQLQESCSVCLTGLDGFRQQQQEDRERLRTLEKLMSSMEERQQQLVGPAMARRPPQECCPPELGRRVSELERRLDVVTGSLTVLSGRRGSELGGAAGQGGHPPGYTSLASRLSRLEDRFNSTLGPSEEQEKNWPGGPGRLGHWLPAAPGRLEKLEGLLANVSRELGGRMDLLEEQVAGAVRTCGQICSGAPGEQDSRVNEILSALERRVLDSEGRLQLVGSGLHEAEAAGEAQQAVLEGLQGLLSRLRERMDAQEETAAEILLRLNLTAAQLSQLEGLLQARGDEGCGACGGVQEELGRLRDGVERCSCPLLPPRGPGAGPGVGGPSRGPLDGFSVFGGSSGSALQALQGELSEVILTFSSLNDSLHELQTTVEGQGADLADLGATKDSIISEINRLQQEATEHVTESEERFRGLEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGLQGLREGLSRHVAGLWAAVRESNSTSLTQAALLEKLLGGQAGLGRRLGALNNSLLLLEDRLQQLSLKDFTGPSGKAGPPGPPGLQGPSGPAGPPGPPGKDGQQGAIGPPGPQGEQGAEGAPAAPVPRVAFSAALSLPRSEPGTVPFDRVLLNDGGYYDPETGVFTAPLAGRYLLSAVLTGHRHEKVEAVLSRSNLGVARIDSGGYEPEGLENKPVAESQPSPGALGVFSLILPLQVGDTVCIDLVMGQLAHSEEPLTIFSGALLYEDTELEQV

Target ORF information:

RefSeq Version	XM_006503648.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503648.3

ORF Insert Sequence:

ATGTACCGGA GCTTTCTTCG CCCTCGCTAT CGGGTGGCTT ACAAGACAGT GACAGACATG 
GAGTGGAGGT GCTGTCAGGG TTATGGGGGC GATGACTGTG GAGAGGGCCC TGCTTCCGTT 
CTGGGCCCAG CACCCTCCAC ACCACTTCCC CGGCCCAGGC CTGTGCGCCC CAACCTGTCT 
GGCTCCAGTG CCGGCAGCCA TCTCAGTGGA CTGGGAGGAG AAGGTCCTGT TGAGTCAGAG 
AAGGTGCAGC AGCTAGAGCG GCAGGTGAAG AGTCTGACCA AAGAGCTGCA AGGCCTTCGG 
GGTGTCCTGC AGGGGATGAA TGGGCGCCTA GCAGAAGATG TACAGCGAGC TGTGGACACA 
GTCTTTAATG GAAGGCAGCA GCCAGCAGAT GCAGCTGCCC GCCCGGGCGT GCATGAAACC 
CTCAGTGAGA TCCAGCAGCA GCTGCAGCTC CTGGACAACC GTGTCTCCAC TCATGACCAG 
GAGCTGGGCC ACCTTAACAA CCATCATAAT GGAGGCCCTG GTGGAGGTGG CAGGGCCTCA 
GGCCCTGTCC CAGTTCCTTC TGGCCCCAGT GAAGAACTGT TAAGGCAGCT GGAACGGCAG 
CTGCAAGAGT CTTGCTCAGT GTGCCTGACG GGGCTGGATG GCTTCCGCCA GCAGCAGCAA 
GAGGATAGGG AGCGGCTGCG AACCCTGGAG AAGCTAATGT CCTCTATGGA GGAGCGGCAG 
CAACAGCTTG TGGGACCTGC CATGGCCAGG AGACCCCCTC AGGAATGCTG CCCCCCAGAG 
CTGGGTCGAC GAGTGTCTGA GCTGGAGCGG AGGCTAGATG TAGTGACTGG CTCACTGACA 
GTGCTAAGTG GACGCAGAGG TTCTGAGCTT GGAGGAGCAG CTGGGCAGGG GGGCCACCCT 
CCAGGCTACA CCAGCTTGGC CTCCCGCCTT TCTCGCCTGG AGGACCGCTT CAACTCTACC 
CTAGGTCCCT CAGAGGAGCA AGAGAAGAAC TGGCCTGGAG GACCAGGGAG GCTGGGCCAC 
TGGTTGCCTG CTGCTCCAGG ACGGCTAGAA AAGCTGGAGG GACTACTAGC CAATGTGAGC 
AGGGAGCTGG GTGGCCGCAT GGATCTGCTG GAAGAGCAGG TGGCAGGGGC TGTTCGGACT 
TGTGGGCAGA TTTGCTCTGG GGCACCCGGG GAACAGGATT CTCGGGTCAA TGAGATCCTC 
AGTGCCTTGG AACGCAGGGT GCTGGACAGC GAGGGCCGGT TACAGCTAGT GGGCTCTGGC 
TTGCATGAAG CAGAGGCAGC AGGGGAGGCT CAGCAGGCCG TGTTGGAGGG ACTGCAAGGG 
CTCCTGAGCC GGCTTCGGGA GCGCATGGAT GCACAGGAGG AGACTGCAGC AGAAATCTTA 
CTGCGCCTCA ATCTTACCGC AGCCCAGCTA AGCCAGCTGG AGGGTCTGCT GCAAGCCCGT 
GGGGATGAGG GTTGTGGCGC CTGTGGTGGT GTCCAGGAGG AGCTGGGCCG CCTTCGGGAT 
GGTGTGGAAC GTTGCTCCTG CCCATTGTTA CCTCCACGGG GCCCTGGAGC TGGCCCAGGG 
GTTGGGGGAC CAAGCCGTGG GCCTCTGGAT GGTTTCAGTG TGTTTGGGGG CAGTTCAGGC 
TCAGCCCTCC AGGCCCTTCA AGGAGAACTC TCTGAGGTTA TTCTCACCTT CAGCTCCCTG 
AACGACTCAC TCCACGAGCT CCAGACCACT GTGGAGGGTC AGGGTGCCGA TCTGGCTGAC 
CTGGGGGCCA CCAAGGACAG CATCATCTCT GAAATCAACA GACTACAGCA GGAGGCCACG 
GAGCACGTCA CAGAGAGCGA GGAGCGCTTC CGAGGCCTGG AGGAGGGCCA GGCACAGGCT 
GGCCAATGCC CTAGCTTAGA GGGGCGATTA GGCCGCCTTG AGGGAGTCTG CGAACGACTG 
GACACCGTGG CAGGGGGACT ACAAGGCCTA CGTGAAGGCC TCTCCAGACA CGTGGCTGGG 
CTCTGGGCTG CAGTACGGGA AAGCAACAGC ACCAGCCTGA CACAGGCCGC CCTGCTGGAG 
AAGCTGCTGG GGGGCCAGGC AGGCCTGGGC AGGCGGCTTG GTGCCCTCAA CAATTCCCTG 
CTGCTCCTGG AAGACCGTCT GCAGCAACTT AGCCTAAAGG ACTTCACTGG ACCTTCAGGC 
AAGGCTGGGC CCCCAGGGCC TCCCGGGCTA CAAGGGCCTT CAGGCCCTGC AGGACCTCCA 
GGACCTCCTG GCAAAGACGG ACAACAGGGG GCCATTGGCC CACCAGGTCC TCAAGGGGAG 
CAGGGAGCGG AGGGAGCACC AGCAGCCCCT GTGCCTAGGG TAGCATTTTC AGCTGCCCTG 
AGTTTGCCAC GGTCAGAACC TGGCACAGTC CCCTTCGACA GAGTCTTGCT CAATGATGGA 
GGCTACTATG ACCCAGAGAC AGGTGTATTC ACTGCACCAC TGGCTGGACG CTATTTGCTG 
AGCGCTGTAC TTACTGGGCA CCGGCATGAG AAAGTGGAAG CAGTATTGTC ACGCTCCAAC 
CTGGGCGTGG CCCGCATAGA CTCGGGAGGC TATGAACCCG AGGGACTGGA GAATAAGCCT 
GTGGCCGAAA GTCAGCCCAG CCCAGGCGCT CTGGGCGTCT TCAGCCTCAT TCTGCCACTG 
CAGGTCGGAG ACACTGTCTG CATCGACCTG GTCATGGGGC AGCTGGCACA CTCCGAGGAG 
CCGCTCACCA TCTTCAGCGG AGCCCTGCTC TACGAGGACA CAGAGCTTGA ACAGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin1 ( XM_006503649.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503712.1 Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34886
Clone ID Related Accession (Same CDS sequence)	XM_006503649.3 , XM_006503649.4
Accession Version	XM_006503649.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	EMILIN-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGC GCCTAGCAGA AGATGTACAG CGAGCTGTGG ACACAGTCTT TAATGGAAGG 
CAGCAGCCAG CAGATGCAGC TGCCCGCCCG GGCGTGCATG AAACCCTCAG TGAGATCCAG 
CAGCAGCTGC AGCTCCTGGA CAACCGTGTC TCCACTCATG ACCAGGAGCT GGGCCACCTT 
AACAACCATC ATAATGGAGG CCCTGGTGGA GGTGGCAGGG CCTCAGGCCC TGTCCCAGTT 
CCTTCTGGCC CCAGTGAAGA ACTGTTAAGG CAGCTGGAAC GGCAGCTGCA AGAGTCTTGC 
TCAGTGTGCC TGACGGGGCT GGATGGCTTC CGCCAGCAGC AGCAAGAGGA TAGGGAGCGG 
CTGCGAACCC TGGAGAAGCT AATGTCCTCT ATGGAGGAGC GGCAGCAACA GCTTGTGGGA 
CCTGCCATGG CCAGGAGACC CCCTCAGGAA TGCTGCCCCC CAGAGCTGGG TCGACGAGTG 
TCTGAGCTGG AGCGGAGGCT AGATGTAGTG ACTGGCTCAC TGACAGTGCT AAGTGGACGC 
AGAGGTTCTG AGCTTGGAGG AGCAGCTGGG CAGGGGGGCC ACCCTCCAGG CTACACCAGC 
TTGGCCTCCC GCCTTTCTCG CCTGGAGGAC CGCTTCAACT CTACCCTAGG TCCCTCAGAG 
GAGCAAGAGA AGAACTGGCC TGGAGGACCA GGGAGGCTGG GCCACTGGTT GCCTGCTGCT 
CCAGGACGGC TAGAAAAGCT GGAGGGACTA CTAGCCAATG TGAGCAGGGA GCTGGGTGGC 
CGCATGGATC TGCTGGAAGA GCAGGTGGCA GGGGCTGTTC GGACTTGTGG GCAGATTTGC 
TCTGGGGCAC CCGGGGAACA GGATTCTCGG GTCAATGAGA TCCTCAGTGC CTTGGAACGC 
AGGGTGCTGG ACAGCGAGGG CCGGTTACAG CTAGTGGGCT CTGGCTTGCA TGAAGCAGAG 
GCAGCAGGGG AGGCTCAGCA GGCCGTGTTG GAGGGACTGC AAGGGCTCCT GAGCCGGCTT 
CGGGAGCGCA TGGATGCACA GGAGGAGACT GCAGCAGAAA TCTTACTGCG CCTCAATCTT 
ACCGCAGCCC AGCTAAGCCA GCTGGAGGGT CTGCTGCAAG CCCGTGGGGA TGAGGGTTGT 
GGCGCCTGTG GTGGTGTCCA GGAGGAGCTG GGCCGCCTTC GGGATGGTGT GGAACGTTGC 
TCCTGCCCAT TGTTACCTCC ACGGGGCCCT GGAGCTGGCC CAGGGGTTGG GGGACCAAGC 
CGTGGGCCTC TGGATGGTTT CAGTGTGTTT GGGGGCAGTT CAGGCTCAGC CCTCCAGGCC 
CTTCAAGGAG AACTCTCTGA GGTTATTCTC ACCTTCAGCT CCCTGAACGA CTCACTCCAC 
GAGCTCCAGA CCACTGTGGA GGGTCAGGGT GCCGATCTGG CTGACCTGGG GGCCACCAAG 
GACAGCATCA TCTCTGAAAT CAACAGACTA CAGCAGGAGG CCACGGAGCA CGTCACAGAG 
AGCGAGGAGC GCTTCCGAGG CCTGGAGGAG GGCCAGGCAC AGGCTGGCCA ATGCCCTAGC 
TTAGAGGGGC GATTAGGCCG CCTTGAGGGA GTCTGCGAAC GACTGGACAC CGTGGCAGGG 
GGACTACAAG GCCTACGTGA AGGCCTCTCC AGACACGTGG CTGGGCTCTG GGCTGCAGTA 
CGGGAAAGCA ACAGCACCAG CCTGACACAG GCCGCCCTGC TGGAGAAGCT GCTGGGGGGC 
CAGGCAGGCC TGGGCAGGCG GCTTGGTGCC CTCAACAATT CCCTGCTGCT CCTGGAAGAC 
CGTCTGCAGC AACTTAGCCT AAAGGACTTC ACTGGACCTT CAGGCAAGGC TGGGCCCCCA 
GGGCCTCCCG GGCTACAAGG GCCTTCAGGC CCTGCAGGAC CTCCAGGACC TCCTGGCAAA 
GACGGACAAC AGGGGGCCAT TGGCCCACCA GGTCCTCAAG GGGAGCAGGG AGCGGAGGGA 
GCACCAGCAG CCCCTGTGCC TAGGGTAGCA TTTTCAGCTG CCCTGAGTTT GCCACGGTCA 
GAACCTGGCA CAGTCCCCTT CGACAGAGTC TTGCTCAATG ATGGAGGCTA CTATGACCCA 
GAGACAGGTG TATTCACTGC ACCACTGGCT GGACGCTATT TGCTGAGCGC TGTACTTACT 
GGGCACCGGC ATGAGAAAGT GGAAGCAGTA TTGTCACGCT CCAACCTGGG CGTGGCCCGC 
ATAGACTCGG GAGGCTATGA ACCCGAGGGA CTGGAGAATA AGCCTGTGGC CGAAAGTCAG 
CCCAGCCCAG GCGCTCTGGG CGTCTTCAGC CTCATTCTGC CACTGCAGGT CGGAGACACT 
GTCTGCATCG ACCTGGTCAT GGGGCAGCTG GCACACTCCG AGGAGCCGCT CACCATCTTC 
AGCGGAGCCC TGCTCTACGA GGACACAGAG CTTGAACAGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503712.1
CDS	756..3200
Translation	MNGRLAEDVQRAVDTVFNGRQQPADAAARPGVHETLSEIQQQLQLLDNRVSTHDQELGHLNNHHNGGPGGGGRASGPVPVPSGPSEELLRQLERQLQESCSVCLTGLDGFRQQQQEDRERLRTLEKLMSSMEERQQQLVGPAMARRPPQECCPPELGRRVSELERRLDVVTGSLTVLSGRRGSELGGAAGQGGHPPGYTSLASRLSRLEDRFNSTLGPSEEQEKNWPGGPGRLGHWLPAAPGRLEKLEGLLANVSRELGGRMDLLEEQVAGAVRTCGQICSGAPGEQDSRVNEILSALERRVLDSEGRLQLVGSGLHEAEAAGEAQQAVLEGLQGLLSRLRERMDAQEETAAEILLRLNLTAAQLSQLEGLLQARGDEGCGACGGVQEELGRLRDGVERCSCPLLPPRGPGAGPGVGGPSRGPLDGFSVFGGSSGSALQALQGELSEVILTFSSLNDSLHELQTTVEGQGADLADLGATKDSIISEINRLQQEATEHVTESEERFRGLEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGLQGLREGLSRHVAGLWAAVRESNSTSLTQAALLEKLLGGQAGLGRRLGALNNSLLLLEDRLQQLSLKDFTGPSGKAGPPGPPGLQGPSGPAGPPGPPGKDGQQGAIGPPGPQGEQGAEGAPAAPVPRVAFSAALSLPRSEPGTVPFDRVLLNDGGYYDPETGVFTAPLAGRYLLSAVLTGHRHEKVEAVLSRSNLGVARIDSGGYEPEGLENKPVAESQPSPGALGVFSLILPLQVGDTVCIDLVMGQLAHSEEPLTIFSGALLYEDTELEQV

Target ORF information:

RefSeq Version	XM_006503649.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503649.3

ORF Insert Sequence:

ATGAATGGGC GCCTAGCAGA AGATGTACAG CGAGCTGTGG ACACAGTCTT TAATGGAAGG 
CAGCAGCCAG CAGATGCAGC TGCCCGCCCG GGCGTGCATG AAACCCTCAG TGAGATCCAG 
CAGCAGCTGC AGCTCCTGGA CAACCGTGTC TCCACTCATG ACCAGGAGCT GGGCCACCTT 
AACAACCATC ATAATGGAGG CCCTGGTGGA GGTGGCAGGG CCTCAGGCCC TGTCCCAGTT 
CCTTCTGGCC CCAGTGAAGA ACTGTTAAGG CAGCTGGAAC GGCAGCTGCA AGAGTCTTGC 
TCAGTGTGCC TGACGGGGCT GGATGGCTTC CGCCAGCAGC AGCAAGAGGA TAGGGAGCGG 
CTGCGAACCC TGGAGAAGCT AATGTCCTCT ATGGAGGAGC GGCAGCAACA GCTTGTGGGA 
CCTGCCATGG CCAGGAGACC CCCTCAGGAA TGCTGCCCCC CAGAGCTGGG TCGACGAGTG 
TCTGAGCTGG AGCGGAGGCT AGATGTAGTG ACTGGCTCAC TGACAGTGCT AAGTGGACGC 
AGAGGTTCTG AGCTTGGAGG AGCAGCTGGG CAGGGGGGCC ACCCTCCAGG CTACACCAGC 
TTGGCCTCCC GCCTTTCTCG CCTGGAGGAC CGCTTCAACT CTACCCTAGG TCCCTCAGAG 
GAGCAAGAGA AGAACTGGCC TGGAGGACCA GGGAGGCTGG GCCACTGGTT GCCTGCTGCT 
CCAGGACGGC TAGAAAAGCT GGAGGGACTA CTAGCCAATG TGAGCAGGGA GCTGGGTGGC 
CGCATGGATC TGCTGGAAGA GCAGGTGGCA GGGGCTGTTC GGACTTGTGG GCAGATTTGC 
TCTGGGGCAC CCGGGGAACA GGATTCTCGG GTCAATGAGA TCCTCAGTGC CTTGGAACGC 
AGGGTGCTGG ACAGCGAGGG CCGGTTACAG CTAGTGGGCT CTGGCTTGCA TGAAGCAGAG 
GCAGCAGGGG AGGCTCAGCA GGCCGTGTTG GAGGGACTGC AAGGGCTCCT GAGCCGGCTT 
CGGGAGCGCA TGGATGCACA GGAGGAGACT GCAGCAGAAA TCTTACTGCG CCTCAATCTT 
ACCGCAGCCC AGCTAAGCCA GCTGGAGGGT CTGCTGCAAG CCCGTGGGGA TGAGGGTTGT 
GGCGCCTGTG GTGGTGTCCA GGAGGAGCTG GGCCGCCTTC GGGATGGTGT GGAACGTTGC 
TCCTGCCCAT TGTTACCTCC ACGGGGCCCT GGAGCTGGCC CAGGGGTTGG GGGACCAAGC 
CGTGGGCCTC TGGATGGTTT CAGTGTGTTT GGGGGCAGTT CAGGCTCAGC CCTCCAGGCC 
CTTCAAGGAG AACTCTCTGA GGTTATTCTC ACCTTCAGCT CCCTGAACGA CTCACTCCAC 
GAGCTCCAGA CCACTGTGGA GGGTCAGGGT GCCGATCTGG CTGACCTGGG GGCCACCAAG 
GACAGCATCA TCTCTGAAAT CAACAGACTA CAGCAGGAGG CCACGGAGCA CGTCACAGAG 
AGCGAGGAGC GCTTCCGAGG CCTGGAGGAG GGCCAGGCAC AGGCTGGCCA ATGCCCTAGC 
TTAGAGGGGC GATTAGGCCG CCTTGAGGGA GTCTGCGAAC GACTGGACAC CGTGGCAGGG 
GGACTACAAG GCCTACGTGA AGGCCTCTCC AGACACGTGG CTGGGCTCTG GGCTGCAGTA 
CGGGAAAGCA ACAGCACCAG CCTGACACAG GCCGCCCTGC TGGAGAAGCT GCTGGGGGGC 
CAGGCAGGCC TGGGCAGGCG GCTTGGTGCC CTCAACAATT CCCTGCTGCT CCTGGAAGAC 
CGTCTGCAGC AACTTAGCCT AAAGGACTTC ACTGGACCTT CAGGCAAGGC TGGGCCCCCA 
GGGCCTCCCG GGCTACAAGG GCCTTCAGGC CCTGCAGGAC CTCCAGGACC TCCTGGCAAA 
GACGGACAAC AGGGGGCCAT TGGCCCACCA GGTCCTCAAG GGGAGCAGGG AGCGGAGGGA 
GCACCAGCAG CCCCTGTGCC TAGGGTAGCA TTTTCAGCTG CCCTGAGTTT GCCACGGTCA 
GAACCTGGCA CAGTCCCCTT CGACAGAGTC TTGCTCAATG ATGGAGGCTA CTATGACCCA 
GAGACAGGTG TATTCACTGC ACCACTGGCT GGACGCTATT TGCTGAGCGC TGTACTTACT 
GGGCACCGGC ATGAGAAAGT GGAAGCAGTA TTGTCACGCT CCAACCTGGG CGTGGCCCGC 
ATAGACTCGG GAGGCTATGA ACCCGAGGGA CTGGAGAATA AGCCTGTGGC CGAAAGTCAG 
CCCAGCCCAG GCGCTCTGGG CGTCTTCAGC CTCATTCTGC CACTGCAGGT CGGAGACACT 
GTCTGCATCG ACCTGGTCAT GGGGCAGCTG GCACACTCCG AGGAGCCGCT CACCATCTTC 
AGCGGAGCCC TGCTCTACGA GGACACAGAG CTTGAACAGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin1 ( XM_006503649.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503712.1 Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34886
Clone ID Related Accession (Same CDS sequence)	XM_006503649.3 , XM_006503649.4
Accession Version	XM_006503649.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	EMILIN-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503649.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGC GCCTAGCAGA AGATGTACAG CGAGCTGTGG ACACAGTCTT TAATGGAAGG 
CAGCAGCCAG CAGATGCAGC TGCCCGCCCG GGCGTGCATG AAACCCTCAG TGAGATCCAG 
CAGCAGCTGC AGCTCCTGGA CAACCGTGTC TCCACTCATG ACCAGGAGCT GGGCCACCTT 
AACAACCATC ATAATGGAGG CCCTGGTGGA GGTGGCAGGG CCTCAGGCCC TGTCCCAGTT 
CCTTCTGGCC CCAGTGAAGA ACTGTTAAGG CAGCTGGAAC GGCAGCTGCA AGAGTCTTGC 
TCAGTGTGCC TGACGGGGCT GGATGGCTTC CGCCAGCAGC AGCAAGAGGA TAGGGAGCGG 
CTGCGAACCC TGGAGAAGCT AATGTCCTCT ATGGAGGAGC GGCAGCAACA GCTTGTGGGA 
CCTGCCATGG CCAGGAGACC CCCTCAGGAA TGCTGCCCCC CAGAGCTGGG TCGACGAGTG 
TCTGAGCTGG AGCGGAGGCT AGATGTAGTG ACTGGCTCAC TGACAGTGCT AAGTGGACGC 
AGAGGTTCTG AGCTTGGAGG AGCAGCTGGG CAGGGGGGCC ACCCTCCAGG CTACACCAGC 
TTGGCCTCCC GCCTTTCTCG CCTGGAGGAC CGCTTCAACT CTACCCTAGG TCCCTCAGAG 
GAGCAAGAGA AGAACTGGCC TGGAGGACCA GGGAGGCTGG GCCACTGGTT GCCTGCTGCT 
CCAGGACGGC TAGAAAAGCT GGAGGGACTA CTAGCCAATG TGAGCAGGGA GCTGGGTGGC 
CGCATGGATC TGCTGGAAGA GCAGGTGGCA GGGGCTGTTC GGACTTGTGG GCAGATTTGC 
TCTGGGGCAC CCGGGGAACA GGATTCTCGG GTCAATGAGA TCCTCAGTGC CTTGGAACGC 
AGGGTGCTGG ACAGCGAGGG CCGGTTACAG CTAGTGGGCT CTGGCTTGCA TGAAGCAGAG 
GCAGCAGGGG AGGCTCAGCA GGCCGTGTTG GAGGGACTGC AAGGGCTCCT GAGCCGGCTT 
CGGGAGCGCA TGGATGCACA GGAGGAGACT GCAGCAGAAA TCTTACTGCG CCTCAATCTT 
ACCGCAGCCC AGCTAAGCCA GCTGGAGGGT CTGCTGCAAG CCCGTGGGGA TGAGGGTTGT 
GGCGCCTGTG GTGGTGTCCA GGAGGAGCTG GGCCGCCTTC GGGATGGTGT GGAACGTTGC 
TCCTGCCCAT TGTTACCTCC ACGGGGCCCT GGAGCTGGCC CAGGGGTTGG GGGACCAAGC 
CGTGGGCCTC TGGATGGTTT CAGTGTGTTT GGGGGCAGTT CAGGCTCAGC CCTCCAGGCC 
CTTCAAGGAG AACTCTCTGA GGTTATTCTC ACCTTCAGCT CCCTGAACGA CTCACTCCAC 
GAGCTCCAGA CCACTGTGGA GGGTCAGGGT GCCGATCTGG CTGACCTGGG GGCCACCAAG 
GACAGCATCA TCTCTGAAAT CAACAGACTA CAGCAGGAGG CCACGGAGCA CGTCACAGAG 
AGCGAGGAGC GCTTCCGAGG CCTGGAGGAG GGCCAGGCAC AGGCTGGCCA ATGCCCTAGC 
TTAGAGGGGC GATTAGGCCG CCTTGAGGGA GTCTGCGAAC GACTGGACAC CGTGGCAGGG 
GGACTACAAG GCCTACGTGA AGGCCTCTCC AGACACGTGG CTGGGCTCTG GGCTGCAGTA 
CGGGAAAGCA ACAGCACCAG CCTGACACAG GCCGCCCTGC TGGAGAAGCT GCTGGGGGGC 
CAGGCAGGCC TGGGCAGGCG GCTTGGTGCC CTCAACAATT CCCTGCTGCT CCTGGAAGAC 
CGTCTGCAGC AACTTAGCCT AAAGGACTTC ACTGGACCTT CAGGCAAGGC TGGGCCCCCA 
GGGCCTCCCG GGCTACAAGG GCCTTCAGGC CCTGCAGGAC CTCCAGGACC TCCTGGCAAA 
GACGGACAAC AGGGGGCCAT TGGCCCACCA GGTCCTCAAG GGGAGCAGGG AGCGGAGGGA 
GCACCAGCAG CCCCTGTGCC TAGGGTAGCA TTTTCAGCTG CCCTGAGTTT GCCACGGTCA 
GAACCTGGCA CAGTCCCCTT CGACAGAGTC TTGCTCAATG ATGGAGGCTA CTATGACCCA 
GAGACAGGTG TATTCACTGC ACCACTGGCT GGACGCTATT TGCTGAGCGC TGTACTTACT 
GGGCACCGGC ATGAGAAAGT GGAAGCAGTA TTGTCACGCT CCAACCTGGG CGTGGCCCGC 
ATAGACTCGG GAGGCTATGA ACCCGAGGGA CTGGAGAATA AGCCTGTGGC CGAAAGTCAG 
CCCAGCCCAG GCGCTCTGGG CGTCTTCAGC CTCATTCTGC CACTGCAGGT CGGAGACACT 
GTCTGCATCG ACCTGGTCAT GGGGCAGCTG GCACACTCCG AGGAGCCGCT CACCATCTTC 
AGCGGAGCCC TGCTCTACGA GGACACAGAG CTTGAACAGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503712.1
CDS	751..3195
Translation	MNGRLAEDVQRAVDTVFNGRQQPADAAARPGVHETLSEIQQQLQLLDNRVSTHDQELGHLNNHHNGGPGGGGRASGPVPVPSGPSEELLRQLERQLQESCSVCLTGLDGFRQQQQEDRERLRTLEKLMSSMEERQQQLVGPAMARRPPQECCPPELGRRVSELERRLDVVTGSLTVLSGRRGSELGGAAGQGGHPPGYTSLASRLSRLEDRFNSTLGPSEEQEKNWPGGPGRLGHWLPAAPGRLEKLEGLLANVSRELGGRMDLLEEQVAGAVRTCGQICSGAPGEQDSRVNEILSALERRVLDSEGRLQLVGSGLHEAEAAGEAQQAVLEGLQGLLSRLRERMDAQEETAAEILLRLNLTAAQLSQLEGLLQARGDEGCGACGGVQEELGRLRDGVERCSCPLLPPRGPGAGPGVGGPSRGPLDGFSVFGGSSGSALQALQGELSEVILTFSSLNDSLHELQTTVEGQGADLADLGATKDSIISEINRLQQEATEHVTESEERFRGLEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGLQGLREGLSRHVAGLWAAVRESNSTSLTQAALLEKLLGGQAGLGRRLGALNNSLLLLEDRLQQLSLKDFTGPSGKAGPPGPPGLQGPSGPAGPPGPPGKDGQQGAIGPPGPQGEQGAEGAPAAPVPRVAFSAALSLPRSEPGTVPFDRVLLNDGGYYDPETGVFTAPLAGRYLLSAVLTGHRHEKVEAVLSRSNLGVARIDSGGYEPEGLENKPVAESQPSPGALGVFSLILPLQVGDTVCIDLVMGQLAHSEEPLTIFSGALLYEDTELEQV

Target ORF information:

RefSeq Version	XM_006503649.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 1 (Emilin1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503649.4

ORF Insert Sequence:

ATGAATGGGC GCCTAGCAGA AGATGTACAG CGAGCTGTGG ACACAGTCTT TAATGGAAGG 
CAGCAGCCAG CAGATGCAGC TGCCCGCCCG GGCGTGCATG AAACCCTCAG TGAGATCCAG 
CAGCAGCTGC AGCTCCTGGA CAACCGTGTC TCCACTCATG ACCAGGAGCT GGGCCACCTT 
AACAACCATC ATAATGGAGG CCCTGGTGGA GGTGGCAGGG CCTCAGGCCC TGTCCCAGTT 
CCTTCTGGCC CCAGTGAAGA ACTGTTAAGG CAGCTGGAAC GGCAGCTGCA AGAGTCTTGC 
TCAGTGTGCC TGACGGGGCT GGATGGCTTC CGCCAGCAGC AGCAAGAGGA TAGGGAGCGG 
CTGCGAACCC TGGAGAAGCT AATGTCCTCT ATGGAGGAGC GGCAGCAACA GCTTGTGGGA 
CCTGCCATGG CCAGGAGACC CCCTCAGGAA TGCTGCCCCC CAGAGCTGGG TCGACGAGTG 
TCTGAGCTGG AGCGGAGGCT AGATGTAGTG ACTGGCTCAC TGACAGTGCT AAGTGGACGC 
AGAGGTTCTG AGCTTGGAGG AGCAGCTGGG CAGGGGGGCC ACCCTCCAGG CTACACCAGC 
TTGGCCTCCC GCCTTTCTCG CCTGGAGGAC CGCTTCAACT CTACCCTAGG TCCCTCAGAG 
GAGCAAGAGA AGAACTGGCC TGGAGGACCA GGGAGGCTGG GCCACTGGTT GCCTGCTGCT 
CCAGGACGGC TAGAAAAGCT GGAGGGACTA CTAGCCAATG TGAGCAGGGA GCTGGGTGGC 
CGCATGGATC TGCTGGAAGA GCAGGTGGCA GGGGCTGTTC GGACTTGTGG GCAGATTTGC 
TCTGGGGCAC CCGGGGAACA GGATTCTCGG GTCAATGAGA TCCTCAGTGC CTTGGAACGC 
AGGGTGCTGG ACAGCGAGGG CCGGTTACAG CTAGTGGGCT CTGGCTTGCA TGAAGCAGAG 
GCAGCAGGGG AGGCTCAGCA GGCCGTGTTG GAGGGACTGC AAGGGCTCCT GAGCCGGCTT 
CGGGAGCGCA TGGATGCACA GGAGGAGACT GCAGCAGAAA TCTTACTGCG CCTCAATCTT 
ACCGCAGCCC AGCTAAGCCA GCTGGAGGGT CTGCTGCAAG CCCGTGGGGA TGAGGGTTGT 
GGCGCCTGTG GTGGTGTCCA GGAGGAGCTG GGCCGCCTTC GGGATGGTGT GGAACGTTGC 
TCCTGCCCAT TGTTACCTCC ACGGGGCCCT GGAGCTGGCC CAGGGGTTGG GGGACCAAGC 
CGTGGGCCTC TGGATGGTTT CAGTGTGTTT GGGGGCAGTT CAGGCTCAGC CCTCCAGGCC 
CTTCAAGGAG AACTCTCTGA GGTTATTCTC ACCTTCAGCT CCCTGAACGA CTCACTCCAC 
GAGCTCCAGA CCACTGTGGA GGGTCAGGGT GCCGATCTGG CTGACCTGGG GGCCACCAAG 
GACAGCATCA TCTCTGAAAT CAACAGACTA CAGCAGGAGG CCACGGAGCA CGTCACAGAG 
AGCGAGGAGC GCTTCCGAGG CCTGGAGGAG GGCCAGGCAC AGGCTGGCCA ATGCCCTAGC 
TTAGAGGGGC GATTAGGCCG CCTTGAGGGA GTCTGCGAAC GACTGGACAC CGTGGCAGGG 
GGACTACAAG GCCTACGTGA AGGCCTCTCC AGACACGTGG CTGGGCTCTG GGCTGCAGTA 
CGGGAAAGCA ACAGCACCAG CCTGACACAG GCCGCCCTGC TGGAGAAGCT GCTGGGGGGC 
CAGGCAGGCC TGGGCAGGCG GCTTGGTGCC CTCAACAATT CCCTGCTGCT CCTGGAAGAC 
CGTCTGCAGC AACTTAGCCT AAAGGACTTC ACTGGACCTT CAGGCAAGGC TGGGCCCCCA 
GGGCCTCCCG GGCTACAAGG GCCTTCAGGC CCTGCAGGAC CTCCAGGACC TCCTGGCAAA 
GACGGACAAC AGGGGGCCAT TGGCCCACCA GGTCCTCAAG GGGAGCAGGG AGCGGAGGGA 
GCACCAGCAG CCCCTGTGCC TAGGGTAGCA TTTTCAGCTG CCCTGAGTTT GCCACGGTCA 
GAACCTGGCA CAGTCCCCTT CGACAGAGTC TTGCTCAATG ATGGAGGCTA CTATGACCCA 
GAGACAGGTG TATTCACTGC ACCACTGGCT GGACGCTATT TGCTGAGCGC TGTACTTACT 
GGGCACCGGC ATGAGAAAGT GGAAGCAGTA TTGTCACGCT CCAACCTGGG CGTGGCCCGC 
ATAGACTCGG GAGGCTATGA ACCCGAGGGA CTGGAGAATA AGCCTGTGGC CGAAAGTCAG 
CCCAGCCCAG GCGCTCTGGG CGTCTTCAGC CTCATTCTGC CACTGCAGGT CGGAGACACT 
GTCTGCATCG ACCTGGTCAT GGGGCAGCTG GCACACTCCG AGGAGCCGCT CACCATCTTC 
AGCGGAGCCC TGCTCTACGA GGACACAGAG CTTGAACAGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin1 ( NM_133918.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598679.1 Mus musculus elastin microfibril interfacer 1 (Emilin1), mRNA.

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CloneID	OMu10358
Clone ID Related Accession (Same CDS sequence)	NM_133918.2
Accession Version	NM_133918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	EMILIN-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC109606.11. On Nov 17, 2006 this sequence version replaced NM_133918.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005481.1, AK088995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCCA GAGCCCTCTG GAGCTGCTAT CTCTGCTGTC TGCTGACCAT AGCCACGGAA 
GCTGCCAGCT ACCCTCCTCG AGGTTACAGC CTCTACACAG GAGGCACTGG GGCCCTGAGT 
CCTGGGGGAC CCCAGGCCCA GAACTCACCA CGGCCTGCCA GCCGCCACAG GAACTGGTGT 
GCCTACGTGG TGACTCGGAC AGTGAGCTGC GTCCTTGAGG ATGGAGTGGA GACCATTGTC 
AAGCCAGACT ATCAACCCTG TGGCTGGGGC CAGCCCCACT GTTCCCGAAG TATCATGTAC 
CGGAGCTTTC TTCGCCCTCG CTATCGGGTG GCTTACAAGA CAGTGACAGA CATGGAGTGG 
AGGTGCTGTC AGGGTTATGG GGGCGATGAC TGTGGAGAGG GCCCTGCTTC CGTTCTGGGC 
CCAGCACCCT CCACACCACT TCCCCGGCCC AGGCCTGTGC GCCCCAACCT GTCTGGCTCC 
AGTGCCGGCA GCCATCTCAG TGGACTGGGA GGAGAAGGTC CTGTTGAGTC AGAGAAGGTG 
CAGCAGCTAG AGCGGCAGGT GAAGAGTCTG ACCAAAGAGC TGCAAGGCCT TCGGGGTGTC 
CTGCAGGGGA TGAATGGGCG CCTAGCAGAA GATGTACAGC GAGCTGTGGA CACAGTCTTT 
AATGGAAGGC AGCAGCCAGC AGATGCAGCT GCCCGCCCGG GCGTGCATGA AACCCTCAGT 
GAGATCCAGC AGCAGCTGCA GCTCCTGGAC AACCGTGTCT CCACTCATGA CCAGGAGCTG 
GGCCACCTTA ACAACCATCA TAATGGAGGC CCTGGTGGAG GTGGCAGGGC CTCAGGCCCT 
GTCCCAGTTC CTTCTGGCCC CAGTGAAGAA CTGTTAAGGC AGCTGGAACG GCAGCTGCAA 
GAGTCTTGCT CAGTGTGCCT GACGGGGCTG GATGGCTTCC GCCAGCAGCA GCAAGAGGAT 
AGGGAGCGGC TGCGAACCCT GGAGAAGCTA ATGTCCTCTA TGGAGGAGCG GCAGCAACAG 
CTTGTGGGAC CTGCCATGGC CAGGAGACCC CCTCAGGAAT GCTGCCCCCC AGAGCTGGGT 
CGACGAGTGT CTGAGCTGGA GCGGAGGCTA GATGTAGTGA CTGGCTCACT GACAGTGCTA 
AGTGGACGCA GAGGTTCTGA GCTTGGAGGA GCAGCTGGGC AGGGGGGCCA CCCTCCAGGC 
TACACCAGCT TGGCCTCCCG CCTTTCTCGC CTGGAGGACC GCTTCAACTC TACCCTAGGT 
CCCTCAGAGG AGCAAGAGAA GAACTGGCCT GGAGGACCAG GGAGGCTGGG CCACTGGTTG 
CCTGCTGCTC CAGGACGGCT AGAAAAGCTG GAGGGACTAC TAGCCAATGT GAGCAGGGAG 
CTGGGTGGCC GCATGGATCT GCTGGAAGAG CAGGTGGCAG GGGCTGTTCG GACTTGTGGG 
CAGATTTGCT CTGGGGCACC CGGGGAACAG GATTCTCGGG TCAATGAGAT CCTCAGTGCC 
TTGGAACGCA GGGTGCTGGA CAGCGAGGGC CGGTTACAGC TAGTGGGCTC TGGCTTGCAT 
GAAGCAGAGG CAGCAGGGGA GGCTCAGCAG GCCGTGTTGG AGGGACTGCA AGGGCTCCTG 
AGCCGGCTTC GGGAGCGCAT GGATGCACAG GAGGAGACTG CAGCAGAAAT CTTACTGCGC 
CTCAATCTTA CCGCAGCCCA GCTAAGCCAG CTGGAGGGTC TGCTGCAAGC CCGTGGGGAT 
GAGGGTTGTG GCGCCTGTGG TGGTGTCCAG GAGGAGCTGG GCCGCCTTCG GGATGGTGTG 
GAACGTTGCT CCTGCCCATT GTTACCTCCA CGGGGCCCTG GAGCTGGCCC AGGGGTTGGG 
GGACCAAGCC GTGGGCCTCT GGATGGTTTC AGTGTGTTTG GGGGCAGTTC AGGCTCAGCC 
CTCCAGGCCC TTCAAGGAGA ACTCTCTGAG GTTATTCTCA CCTTCAGCTC CCTGAACGAC 
TCACTCCACG AGCTCCAGAC CACTGTGGAG GGTCAGGGTG CCGATCTGGC TGACCTGGGG 
GCCACCAAGG ACAGCATCAT CTCTGAAATC AACAGACTAC AGCAGGAGGC CACGGAGCAC 
GTCACAGAGA GCGAGGAGCG CTTCCGAGGC CTGGAGGAGG GCCAGGCACA GGCTGGCCAA 
TGCCCTAGCT TAGAGGGGCG ATTAGGCCGC CTTGAGGGAG TCTGCGAACG ACTGGACACC 
GTGGCAGGGG GACTACAAGG CCTACGTGAA GGCCTCTCCA GACACGTGGC TGGGCTCTGG 
GCTGCAGTAC GGGAAAGCAA CAGCACCAGC CTGACACAGG CCGCCCTGCT GGAGAAGCTG 
CTGGGGGGCC AGGCAGGCCT GGGCAGGCGG CTTGGTGCCC TCAACAATTC CCTGCTGCTC 
CTGGAAGACC GTCTGCAGCA ACTTAGCCTA AAGGACTTCA CTGGACCTTC AGGCAAGGCT 
GGGCCCCCAG GGCCTCCCGG GCTACAAGGG CCTTCAGGCC CTGCAGGACC TCCAGGACCT 
CCTGGCAAAG ACGGACAACA GGGGGCCATT GGCCCACCAG GTCCTCAAGG GGAGCAGGGA 
GCGGAGGGAG CACCAGCAGC CCCTGTGCCT AGGGTAGCAT TTTCAGCTGC CCTGAGTTTG 
CCACGGTCAG AACCTGGCAC AGTCCCCTTC GACAGAGTCT TGCTCAATGA TGGAGGCTAC 
TATGACCCAG AGACAGGTGT ATTCACTGCA CCACTGGCTG GACGCTATTT GCTGAGCGCT 
GTACTTACTG GGCACCGGCA TGAGAAAGTG GAAGCAGTAT TGTCACGCTC CAACCTGGGC 
GTGGCCCGCA TAGACTCGGG AGGCTATGAA CCCGAGGGAC TGGAGAATAA GCCTGTGGCC 
GAAAGTCAGC CCAGCCCAGG CGCTCTGGGC GTCTTCAGCC TCATTCTGCC ACTGCAGGTC 
GGAGACACTG TCTGCATCGA CCTGGTCATG GGGCAGCTGG CACACTCCGA GGAGCCGCTC 
ACCATCTTCA GCGGAGCCCT GCTCTACGAG GACACAGAGC TTGAACAGGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598679.1
CDS	90..3143
Translation	MAPRALWSCYLCCLLTIATEAASYPPRGYSLYTGGTGALSPGGPQAQNSPRPASRHRNWCAYVVTRTVSCVLEDGVETIVKPDYQPCGWGQPHCSRSIMYRSFLRPRYRVAYKTVTDMEWRCCQGYGGDDCGEGPASVLGPAPSTPLPRPRPVRPNLSGSSAGSHLSGLGGEGPVESEKVQQLERQVKSLTKELQGLRGVLQGMNGRLAEDVQRAVDTVFNGRQQPADAAARPGVHETLSEIQQQLQLLDNRVSTHDQELGHLNNHHNGGPGGGGRASGPVPVPSGPSEELLRQLERQLQESCSVCLTGLDGFRQQQQEDRERLRTLEKLMSSMEERQQQLVGPAMARRPPQECCPPELGRRVSELERRLDVVTGSLTVLSGRRGSELGGAAGQGGHPPGYTSLASRLSRLEDRFNSTLGPSEEQEKNWPGGPGRLGHWLPAAPGRLEKLEGLLANVSRELGGRMDLLEEQVAGAVRTCGQICSGAPGEQDSRVNEILSALERRVLDSEGRLQLVGSGLHEAEAAGEAQQAVLEGLQGLLSRLRERMDAQEETAAEILLRLNLTAAQLSQLEGLLQARGDEGCGACGGVQEELGRLRDGVERCSCPLLPPRGPGAGPGVGGPSRGPLDGFSVFGGSSGSALQALQGELSEVILTFSSLNDSLHELQTTVEGQGADLADLGATKDSIISEINRLQQEATEHVTESEERFRGLEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGLQGLREGLSRHVAGLWAAVRESNSTSLTQAALLEKLLGGQAGLGRRLGALNNSLLLLEDRLQQLSLKDFTGPSGKAGPPGPPGLQGPSGPAGPPGPPGKDGQQGAIGPPGPQGEQGAEGAPAAPVPRVAFSAALSLPRSEPGTVPFDRVLLNDGGYYDPETGVFTAPLAGRYLLSAVLTGHRHEKVEAVLSRSNLGVARIDSGGYEPEGLENKPVAESQPSPGALGVFSLILPLQVGDTVCIDLVMGQLAHSEEPLTIFSGALLYEDTELEQV

Target ORF information:

RefSeq Version	NM_133918.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 1 (Emilin1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133918.2

ORF Insert Sequence:

ATGGCCCCCA GAGCCCTCTG GAGCTGCTAT CTCTGCTGTC TGCTGACCAT AGCCACGGAA 
GCTGCCAGCT ACCCTCCTCG AGGTTACAGC CTCTACACAG GAGGCACTGG GGCCCTGAGT 
CCTGGGGGAC CCCAGGCCCA GAACTCACCA CGGCCTGCCA GCCGCCACAG GAACTGGTGT 
GCCTACGTGG TGACTCGGAC AGTGAGCTGC GTCCTTGAGG ATGGAGTGGA GACCATTGTC 
AAGCCAGACT ATCAACCCTG TGGCTGGGGC CAGCCCCACT GTTCCCGAAG TATCATGTAC 
CGGAGCTTTC TTCGCCCTCG CTATCGGGTG GCTTACAAGA CAGTGACAGA CATGGAGTGG 
AGGTGCTGTC AGGGTTATGG GGGCGATGAC TGTGGAGAGG GCCCTGCTTC CGTTCTGGGC 
CCAGCACCCT CCACACCACT TCCCCGGCCC AGGCCTGTGC GCCCCAACCT GTCTGGCTCC 
AGTGCCGGCA GCCATCTCAG TGGACTGGGA GGAGAAGGTC CTGTTGAGTC AGAGAAGGTG 
CAGCAGCTAG AGCGGCAGGT GAAGAGTCTG ACCAAAGAGC TGCAAGGCCT TCGGGGTGTC 
CTGCAGGGGA TGAATGGGCG CCTAGCAGAA GATGTACAGC GAGCTGTGGA CACAGTCTTT 
AATGGAAGGC AGCAGCCAGC AGATGCAGCT GCCCGCCCGG GCGTGCATGA AACCCTCAGT 
GAGATCCAGC AGCAGCTGCA GCTCCTGGAC AACCGTGTCT CCACTCATGA CCAGGAGCTG 
GGCCACCTTA ACAACCATCA TAATGGAGGC CCTGGTGGAG GTGGCAGGGC CTCAGGCCCT 
GTCCCAGTTC CTTCTGGCCC CAGTGAAGAA CTGTTAAGGC AGCTGGAACG GCAGCTGCAA 
GAGTCTTGCT CAGTGTGCCT GACGGGGCTG GATGGCTTCC GCCAGCAGCA GCAAGAGGAT 
AGGGAGCGGC TGCGAACCCT GGAGAAGCTA ATGTCCTCTA TGGAGGAGCG GCAGCAACAG 
CTTGTGGGAC CTGCCATGGC CAGGAGACCC CCTCAGGAAT GCTGCCCCCC AGAGCTGGGT 
CGACGAGTGT CTGAGCTGGA GCGGAGGCTA GATGTAGTGA CTGGCTCACT GACAGTGCTA 
AGTGGACGCA GAGGTTCTGA GCTTGGAGGA GCAGCTGGGC AGGGGGGCCA CCCTCCAGGC 
TACACCAGCT TGGCCTCCCG CCTTTCTCGC CTGGAGGACC GCTTCAACTC TACCCTAGGT 
CCCTCAGAGG AGCAAGAGAA GAACTGGCCT GGAGGACCAG GGAGGCTGGG CCACTGGTTG 
CCTGCTGCTC CAGGACGGCT AGAAAAGCTG GAGGGACTAC TAGCCAATGT GAGCAGGGAG 
CTGGGTGGCC GCATGGATCT GCTGGAAGAG CAGGTGGCAG GGGCTGTTCG GACTTGTGGG 
CAGATTTGCT CTGGGGCACC CGGGGAACAG GATTCTCGGG TCAATGAGAT CCTCAGTGCC 
TTGGAACGCA GGGTGCTGGA CAGCGAGGGC CGGTTACAGC TAGTGGGCTC TGGCTTGCAT 
GAAGCAGAGG CAGCAGGGGA GGCTCAGCAG GCCGTGTTGG AGGGACTGCA AGGGCTCCTG 
AGCCGGCTTC GGGAGCGCAT GGATGCACAG GAGGAGACTG CAGCAGAAAT CTTACTGCGC 
CTCAATCTTA CCGCAGCCCA GCTAAGCCAG CTGGAGGGTC TGCTGCAAGC CCGTGGGGAT 
GAGGGTTGTG GCGCCTGTGG TGGTGTCCAG GAGGAGCTGG GCCGCCTTCG GGATGGTGTG 
GAACGTTGCT CCTGCCCATT GTTACCTCCA CGGGGCCCTG GAGCTGGCCC AGGGGTTGGG 
GGACCAAGCC GTGGGCCTCT GGATGGTTTC AGTGTGTTTG GGGGCAGTTC AGGCTCAGCC 
CTCCAGGCCC TTCAAGGAGA ACTCTCTGAG GTTATTCTCA CCTTCAGCTC CCTGAACGAC 
TCACTCCACG AGCTCCAGAC CACTGTGGAG GGTCAGGGTG CCGATCTGGC TGACCTGGGG 
GCCACCAAGG ACAGCATCAT CTCTGAAATC AACAGACTAC AGCAGGAGGC CACGGAGCAC 
GTCACAGAGA GCGAGGAGCG CTTCCGAGGC CTGGAGGAGG GCCAGGCACA GGCTGGCCAA 
TGCCCTAGCT TAGAGGGGCG ATTAGGCCGC CTTGAGGGAG TCTGCGAACG ACTGGACACC 
GTGGCAGGGG GACTACAAGG CCTACGTGAA GGCCTCTCCA GACACGTGGC TGGGCTCTGG 
GCTGCAGTAC GGGAAAGCAA CAGCACCAGC CTGACACAGG CCGCCCTGCT GGAGAAGCTG 
CTGGGGGGCC AGGCAGGCCT GGGCAGGCGG CTTGGTGCCC TCAACAATTC CCTGCTGCTC 
CTGGAAGACC GTCTGCAGCA ACTTAGCCTA AAGGACTTCA CTGGACCTTC AGGCAAGGCT 
GGGCCCCCAG GGCCTCCCGG GCTACAAGGG CCTTCAGGCC CTGCAGGACC TCCAGGACCT 
CCTGGCAAAG ACGGACAACA GGGGGCCATT GGCCCACCAG GTCCTCAAGG GGAGCAGGGA 
GCGGAGGGAG CACCAGCAGC CCCTGTGCCT AGGGTAGCAT TTTCAGCTGC CCTGAGTTTG 
CCACGGTCAG AACCTGGCAC AGTCCCCTTC GACAGAGTCT TGCTCAATGA TGGAGGCTAC 
TATGACCCAG AGACAGGTGT ATTCACTGCA CCACTGGCTG GACGCTATTT GCTGAGCGCT 
GTACTTACTG GGCACCGGCA TGAGAAAGTG GAAGCAGTAT TGTCACGCTC CAACCTGGGC 
GTGGCCCGCA TAGACTCGGG AGGCTATGAA CCCGAGGGAC TGGAGAATAA GCCTGTGGCC 
GAAAGTCAGC CCAGCCCAGG CGCTCTGGGC GTCTTCAGCC TCATTCTGCC ACTGCAGGTC 
GGAGACACTG TCTGCATCGA CCTGGTCATG GGGCAGCTGG CACACTCCGA GGAGCCGCTC 
ACCATCTTCA GCGGAGCCCT GCTCTACGAG GACACAGAGC TTGAACAGGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of EMILIN-1 Enhances Arteriolar Myogenic Tone Through TGF-β (Transforming Growth Factor-β)-Dependent Transactivation of EGFR (Epidermal Growth Factor Receptor) and Is Relevant for Hypertension in Mice and Humans.
Arteriosclerosis, thrombosis, and vascular biology38(10)2484-2497(2018 10)
Carnevale D,Facchinello N,Iodice D,Bizzotto D,Perrotta M,De Stefani D,Pallante F,Carnevale L,Ricciardi F,Cifelli G,Da Ros F,Casaburo M,Fardella S,Bonaldo P,Innocenzi G,Rizzuto R,Braghetta P,Lembo G,Bressan GM

Lhx8 ablation leads to massive autophagy of mouse oocytes associated with DNA damage.
Biology of reproduction98(4)532-542(2018 04)
D'Ignazio L,Michel M,Beyer M,Thompson K,Forabosco A,Schlessinger D,Pelosi E

Altered feto-placental vascularization, feto-placental malperfusion and fetal growth restriction in mice with
Development (Cambridge, England)144(13)2469-2479(2017 07)
Lacko LA,Hurtado R,Hinds S,Poulos MG,Butler JM,Stuhlmann H

Fibulin-4 deposition requires EMILIN-1 in the extracellular matrix of osteoblasts.
Scientific reports7(1)5526(2017 07)
Schiavinato A,Keene DR,Imhof T,Doliana R,Sasaki T,Sengle G

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 01)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

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Emilin1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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