atg3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	atg3
Entrez Gene ID	336632
Full Name	autophagy related 3
Synonyms	apg3l,im:6910145,wu:fb15g12,zgc:64156
General protein information	Preferred Names autophagy related 3 Names ubiquitin-like-conjugating enzyme ATG3 APG3 autophagy 3-like APG3-like autophagy-related protein 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200022.1	NP_956316.1	ubiquitin-like-conjugating enzyme ATG3

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATG3	NP_071933.2
Pan troglodytes (chimpanzee)	ATG3	XP_001155234.1
Danio rerio (zebrafish)	apg3l	NP_956316.1
Rattus norvegicus (Norway rat)	Atg3	NP_599221.1
Drosophila melanogaster (fruit fly)	Aut1	NP_649059.1
Arabidopsis thaliana (thale cress)	ATG3	NP_568934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011582	XP_309926.3
Bos taurus (cattle)	ATG3	NP_001068832.1
Canis lupus familiaris (dog)	ATG3	XP_535740.3
Xenopus tropicalis (tropical clawed frog)	atg3	NP_001011420.1
Oryza sativa (rice)	Os01g0200000	NP_001042313.1
Macaca mulatta (Rhesus monkey)	ATG3	XP_001104361.1
Mus musculus (house mouse)	Atg3	NP_080678.1
Gallus gallus (chicken)	ATG3	NP_001264999.1
Caenorhabditis elegans (roundworm)	atg-3	NP_500024.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatotoxicity Induced by Isoniazid-Lipopolysaccharide through Endoplasmic Reticulum Stress, Autophagy, and Apoptosis Pathways in Zebrafish.
Zhang Y, Cen J, Jia Z, Hsiao CD, Xia Q, Wang X, Chen X, Wang R, Jiang Z, Zhang L, Liu K
Antimicrobial agents and chemotherapy63(5)(2019 05)

Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy.
Hu Z, Chen B, Zhao Q
Biology open8(5)(2019 May)

A protective role of autophagy in TDCIPP-induced developmental neurotoxicity in zebrafish larvae.
Li R, Zhang L, Shi Q, Guo Y, Zhang W, Zhou B
Aquatic toxicology (Amsterdam, Netherlands)19946-54(2018 Jun)

Developmental toxicity induced by PM2.5 through endoplasmic reticulum stress and autophagy pathway in zebrafish embryos.
Zhang Y, Li S, Li J, Han L, He Q, Wang R, Wang X, Liu K
Chemosphere197611-621(2018 Apr)

Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish.
Chen B, Zheng YM, Zhang JP
Frontiers in endocrinology9366(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following atg3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atg3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03848	NM_200022.1 Latest version!	Danio rerio autophagy related 3 (atg3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atg3 ( NM_200022.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956316.1 Danio rerio autophagy related 3 (atg3), mRNA.

Price & Availability↑

CloneID	ODa03848
Clone ID Related Accession (Same CDS sequence)	NM_200022.1
Accession Version	NM_200022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Danio rerio(zebrafish)
Product	ubiquitin-like-conjugating enzyme ATG3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057434.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057434.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGAACG TGATAAACTC GGTGAAAGGC ACTGCTCTGG GGGTCGCGGA GTTTTTGACG 
CCAGTTTTGA AGGAATCTAA ATTTAAAGAA ACTGGGGTTA TAACTCCAGA GGAGTTTGTT 
GCAGCAGGAG ATCATCTGGT TCATCACTGT CCCACATGGA AATGGGCATC TGGAGAAGAA 
GCAAAGGTGA AGCCCTATCT GCCCAATGAC AAACAGTTCC TATTAACTCG AAACGTTCCA 
TGTTATAAGC GCTGTAAACA GATGGAGTAC TCAGATGAGC TGGAGGCCAT CATAGAAGAG 
GACGATGGAG ATGGCGGATG GGTGGACACC TTTCATAACT CGGGTGTTAC AGGGGTGACT 
GAAGCTGTTC GGGAAATCTC ATTGGATAAT AAGGATAATA TGAATATGAA TGTGAAGACG 
GGTGCCTGTG GAAATAGTGG AGATGACGAT GATGATGAAG AAGGAGAGGC GGCAGACATG 
GAAGAATATG AAGAAAGTGG ACTTTTGGAA ACAGATGATG CCACTCTTGA CACAAGTAAA 
ATGGCTGATT TAAGTAAAAC TAAGGCCGAA GCTGGAGGGG AAGATGCCAT TCTACAGACA 
AGAACTTATG ACCTGTATAT CACATATGAC AAATATTACC AGACCCCAAG ACTATGGCTG 
TTTGGATATG ATGAGGACAG ACAGCCTCTA ACTGTGGATC AGATGTATGA AGACATCAGC 
CAGGATCATG TTAAAAAAAC CGTCACCATT GAGAATCACC CTAATCTCCC TCCACCTGCT 
ATGTGCTCCG TACATCCATG CAGACACGCT GAGGTGATGA AAAAGATTAT CGAGACTGTG 
GCGGAAGGAG GAGGTGAACT CGGGGTCCAT ATGTATCTTC TGATTTTCCT GAAATTTGTG 
CAAGCTGTCA TTCCAACAAT AGAGTACGAT TACACAAGGC ATTTCACCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956316.1
CDS	115..1068
Translation	MQNVINSVKGTALGVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKWASGEEAKVKPYLPNDKQFLLTRNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTFHNSGVTGVTEAVREISLDNKDNMNMNVKTGACGNSGDDDDDEEGEAADMEEYEESGLLETDDATLDTSKMADLSKTKAEAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEDRQPLTVDQMYEDISQDHVKKTVTIENHPNLPPPAMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM

Target ORF information:

RefSeq Version	NM_200022.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio autophagy related 3 (atg3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200022.1

ORF Insert Sequence:

ATGCAGAACG TGATAAACTC GGTGAAAGGC ACTGCTCTGG GGGTCGCGGA GTTTTTGACG 
CCAGTTTTGA AGGAATCTAA ATTTAAAGAA ACTGGGGTTA TAACTCCAGA GGAGTTTGTT 
GCAGCAGGAG ATCATCTGGT TCATCACTGT CCCACATGGA AATGGGCATC TGGAGAAGAA 
GCAAAGGTGA AGCCCTATCT GCCCAATGAC AAACAGTTCC TATTAACTCG AAACGTTCCA 
TGTTATAAGC GCTGTAAACA GATGGAGTAC TCAGATGAGC TGGAGGCCAT CATAGAAGAG 
GACGATGGAG ATGGCGGATG GGTGGACACC TTTCATAACT CGGGTGTTAC AGGGGTGACT 
GAAGCTGTTC GGGAAATCTC ATTGGATAAT AAGGATAATA TGAATATGAA TGTGAAGACG 
GGTGCCTGTG GAAATAGTGG AGATGACGAT GATGATGAAG AAGGAGAGGC GGCAGACATG 
GAAGAATATG AAGAAAGTGG ACTTTTGGAA ACAGATGATG CCACTCTTGA CACAAGTAAA 
ATGGCTGATT TAAGTAAAAC TAAGGCCGAA GCTGGAGGGG AAGATGCCAT TCTACAGACA 
AGAACTTATG ACCTGTATAT CACATATGAC AAATATTACC AGACCCCAAG ACTATGGCTG 
TTTGGATATG ATGAGGACAG ACAGCCTCTA ACTGTGGATC AGATGTATGA AGACATCAGC 
CAGGATCATG TTAAAAAAAC CGTCACCATT GAGAATCACC CTAATCTCCC TCCACCTGCT 
ATGTGCTCCG TACATCCATG CAGACACGCT GAGGTGATGA AAAAGATTAT CGAGACTGTG 
GCGGAAGGAG GAGGTGAACT CGGGGTCCAT ATGTATCTTC TGATTTTCCT GAAATTTGTG 
CAAGCTGTCA TTCCAACAAT AGAGTACGAT TACACAAGGC ATTTCACCAT GTAG