SMG1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMG1
Entrez Gene ID	525143
Full Name	SMG1, nonsense mediated mRNA decay associated PI3K related kinase
General protein information	Preferred Names SMG1, nonsense mediated mRNA decay associated PI3K related kinase Names serine/threonine-protein kinase SMG1 SMG1 homolog, phosphatidylinositol 3-kinase-related kinase smg-1 homolog, phosphatidylinositol 3-kinase-related kinase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984919.1	XP_024840687.1	serine/threonine-protein kinase SMG1 isoform X2
XM_005224744.4	XP_005224801.1	serine/threonine-protein kinase SMG1 isoform X1
NM_001205915.1	NP_001192844.1	serine/threonine-protein kinase SMG1
XM_005224744.3	XP_005224801.1	serine/threonine-protein kinase SMG1 isoform X1
XM_005224745.3	XP_005224802.1	serine/threonine-protein kinase SMG1 isoform X2
XM_005224745.4	XP_005224802.1	serine/threonine-protein kinase SMG1 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMG1	XP_851552.2
Bos taurus (cattle)	SMG1	NP_001192844.1
Pan troglodytes (chimpanzee)	SMG1	XP_001152224.2
Homo sapiens (human)	SMG1	NP_055907.3
Rattus norvegicus (Norway rat)	Smg1	XP_002728837.2
Gallus gallus (chicken)	SMG1	XP_414907.3
Mus musculus (house mouse)	Smg1	NP_001026984.1
Macaca mulatta (Rhesus monkey)	LOC693542	XP_002808335.1
Danio rerio (zebrafish)	smg1	NP_001073513.2
Xenopus tropicalis (tropical clawed frog)	smg1	XP_002932267.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa37723	NM_001205915.1 Latest version!	Bos taurus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa230306	XM_024984919.1 Latest version!	Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa32978	XM_005224744.4 Latest version!	Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa32979	XM_005224745.4 Latest version!	Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OBa32978	XM_005224744.3	Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa32979	XM_005224745.3	Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMG1 ( NM_001205915.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192844.1 Bos taurus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.

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CloneID	OBa37723
Clone ID Related Accession (Same CDS sequence)	NM_001205915.1
Accession Version	NM_001205915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548691200000
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase SMG1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Apr 20, 2011 this sequence version replaced XM_603490.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATAATTTA 
AAATACTCTT CATCCAGAGA TAGGGGTGGG TCTTCCTCTT ATGGACTGCA GCCTTCAAAT 
TCAGCTGTGG TGTCTCGGCA AAGGCATGAT GATACCAGAA TCCATGCTGA CATACAGAAT 
GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG 
TCGTTGGGGC AAGAGCTGAG GGTTAACAAT GTGACCAGCC CTGAGTTTAC CAGTATTCAG 
CATGGCAGTC GTGCGTTAGC CATCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCT 
TATTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA 
GACCGAAGGC TGGCTACTGT TAAGCAGTTG AAAGAATTTA TTCAGCAACC AGAAAATAAG 
CTGGTGCTAG TTAAGCAGTT GGATAATATC TTGGCTGCTG TACATGATGT GCTTAATGAA 
AGTAGCAAAT TGCTTCAGGA GTTGAGACAG GAGGGAGCTT GTTGTCTTGG CCTTCTTTGT 
GCATCTCTGA GCTATGAGGC TGAGAAGATC TTCAAATGGA TTTTTAGCAA ATTTAGCTCA 
TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC TTATGTGCCA CCTACAAAGC ACTAGAGACT 
GTAGGAGAAA AGAAAGCCTT TTCATCTGTA ATGCAGCTTG TAATGACCAG CCTGCAGTCC 
ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAGT GTGTTAAGTG CATTCTTTTG 
GTAGCTCGAT GTTACCCTCA TATTTTCAGC ACAAATTTTA GGGATACAGT TGATATATTA 
GTTGGATGGC ATATAGATCA TACTCAGAAG CCTTCACTTA CACAACAGGT GTCTGGGTGG 
TTGCAGAGTT TGGAGCCATT CTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT 
CAGTTTTTAG AGGACATGGA GGCATATGCT GAGGACCTCA GCCATGTAGC CTCTGGGGAG 
TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCCTTAC CAAAGCTGGC AGCACTTCTC 
CGGGTATTCA GTACTGTGGT GAGAAGCATT GGGGAACGTT TCAGCCCAAT TCGAGGTCCT 
CCAATTACTG AGGCATATGT AACAGATGTT CTGTACAGAG TAATGAGATG TGTGACGGCT 
GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT 
TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTTAT TACATATGGA 
TTAGATCAAC TGGAGAATTG CCAGACTTGT GGTACAGATT ATATCATCTC AGTCTTGAAT 
TTACTCACAT TGATTGTTGA ACAGATAAAT ACAAAACTGC CATCATCATT TGTAGAAAAA 
CTGTTTATAC CATCATCTAA GCTACTATTT TTGCGTTATC ATAAAGAAAA AGAGGTTGTT 
GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG CTCAGTCTGA AGAATATTCC TGTTTTAGAG 
ACTGCCTATA AATTAATTTT GGGAGAAATG ACTTGTGCCC TAAACAATCT GCTACATAGT 
CTACAACTTC CTGATGCCTG TTCTGAAATA AAACATGAGG CTTTTAAGAA TCATGTTTTC 
AATGTAGACA ATGCAAAATT TGTAGTTATA TTTGACCTTA GTGCTCTGAC TACAATTGGA 
AATGCTAAAA ACTCACTAAT TGGGATGTGG GCCCTGTCTC CAACTGTGTT TGCACTTCTG 
AGTAAGAATC TGATGATTGT GCATGGCGAC CTGGCTGTTC ATTTCCCTGC CATTCAGTAT 
GCAGTGCTCT ACACATTGTA TTCTCATTGT ACCAGGCATG ACCACTTTAT ATCTAGTAGT 
CTTAGTTCAT CATCCCCGTC CTTGTTTGAT GGTGCCGTTA TTAGTACTGT AACTACAGCT 
ACAAAAAAAC ATTTCTCAAT TATCTTAAAT CTCCTGGGAA TATTGCTTAA GAAAGATAAT 
CTTAACCAGG ACACAAGGAA ACTGTTAATG ACCTGGGCTT TGGAAGTGGC TGTTTTAATG 
AAGAAGTCTG AAACATATGC ACCTTTATTC TCTCTTCCAT CTTTCCATAA ATTTTGCAAA 
GGCCTTTTAG CCAACACTCT GGTCGAAGAT GTGAATATCT GTCTGCAAGC ATGCAGCAGT 
CTACATGCAC TGTCCTCTTC CTTACCAGAT GATCTTTTAC AGAGGTGTGT TGATGTTTGC 
CGTGTTCAGC TAGTCCATAG TGGAACTCGT ATTCGACAAG CATTTGGAAA GCTATTGAAG 
TCAATCCCTT TAGATGTTGT CTTGAGCAAT AACAATCACA CAGAAATTCA AGAAATTTCT 
TTAGCATTAA GAAGTCACAT GAGTAAAGCA CCAAGTAACA CATTCCACCC ACAAGATTTC 
TCTGATGTTA TTAGTTTTAT TTTGTATGGA AACTCTCATA GAATAGGGAA GGACAATTGG 
TTAGAAAGAT TGTTCTATAG CTGCCAGAGA CTGGATAAAC GTGACCAATC AACGATTCCC 
CGCAATCTGC TAAAGACAGA TGCTGTCCTC TGGCAGTGGG CTATTTGGGA AGCAGCACAG 
TTCACTGTCC TTTCTAAGCT GAGGACCCCA CTTGGCAGAG CTCAGGATAC CTTCCAAACA 
ATTGAAGGTA TCATTAGAAG TCTTGCAGCT CACACATTAA ACCCCGATCA AGATGTTAGT 
CAGTGGACAA CTGCAGACAA TGACGAAGGC CACAGTAGCA ACCAGCTTAG ACTTGTGCTG 
CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG TTAATGTATA ATGCATATGA AGGATGTGCC 
AATGCATTAA CATCGCCTCC CAAGGTCATT AGGACTTTTT TCTATACCAA TCGCCAAACT 
TGTCAAGACT GGCTGACTCG GATCCGACTC TCCATCATGA GGGTTGGATT GTTGGCAGGC 
CAGCCTGCTG TGACAGTGAG ACACGGCTTC GATTTGCTTA CAGAAATGAA GACAAATCCG 
TCTCAGGGAA ATGAATTGGA AGTAACTATC ATGATGGTGG TAGAAGCATT GTGTGAACTT 
CATTGTCCTG AAGCTATACA GGGAATTGCT GTCTGGTCGT CTTCGGTTGT TGGAAAAAAT 
CTTCTCTGGA TTAATTCAGT GGCCCAACAG GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG 
GAGTACCAGG AGCACCTGTG TGCCATGACA GGTGTCGATT GCTGCATCTC TAGCTTCGAC 
AAATCCGTTC TCACATTGGC TAATACTGGG CGTAATAGTG CCAGCCCCAA GCATTCTTTG 
AATGGTGAAT CCAGAAAAAC TGTGCTTTCC AAACCAACTG ATTCTTCTCC TGAGGTTATA 
AATTATTTGG GAAATAAAGC ATGTGAGTGC TACATTTCAA TAGCTGATTG GGCTGCTGTA 
CAGGAGTGGC AGAATGCTGT CCATGACTTG AAAAAGAGTA CCAGTAGTAC TTCTCTCAAC 
CTAAAGGCTG ATTTCAACTA CATAAAATCA CTAAGCAGTT TTGAATCTGG AGAATTTATT 
GAATGCACTG AACAATTAGA ATTGTTACCA GGAGAAAATA TCAGTCTACT TGCTGGAGGA 
TCAAAAGAAA AAATAGATAT GAAAAAACTG CTTCCTAACA TGTTAAGTCC AGATCCAAGA 
GAACTTCAGA AATCCATTGA AGTTCAGTTG TTAAGAAGTT CTGTTTGTTT GGCAACTGCT 
TTAAACCACA TAGAACAAGA TCAGAAGTGG CAGTCTATTA CCGAAAATGT GGTGAAGTAC 
CTGAAGCAAA CCTCCCGCAT AGCTGTTGGA CCCCTGAGAC TTTCTACTCT AACAGTTTCC 
CAGTCTCTGC CAGTCCTAAG TACTTTACAG CTGTATTGCT CTTCTGCTTT GGAGAACACA 
GTTTCCAACA GACTTGCCAC AGAGGACTGT CTTATTCCAC TCTTTAGTGA AGCTTTGCGT 
TCATGTAAAC AGCATGATGT CAGGCCATGG ATGCAAGCAT TAAGATACAC GATGTACCAG 
AGTCAGTTGT TGGAGAAAAT AAAAGAACAA ACAGTTCCAA TTAGAAGCCA TCTCATGGAA 
TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA AAACGGGGGA ATGTGTCACT TGCAACAAGA 
TTGCTGGCAC AGTGTAGCGA GGTTCAGCTG GGAAAGACTA CCACTGCACA GGATTTAGTC 
CAGCACTTTA AAAAACTATC AACTCAAGGT CAAGTGGATG AAAAATGGGG GCCTGAACTT 
GACATTGAAA AAACGAAACT GCTATATACA GCAGGCCAGT CAACACATGC AATGGAGATG 
TTGAGTTCCT GTGCCATCTC TTTCTGCAAG TCTGCCAAAG CTGAGCATGC TGTTGCCAAG 
TCAATTTTGA CATTGGCTAA ATGGATCCAG GCAGAATGGA AAGAAATCTC AGGACAGCTG 
AAACAGGTTT ACAGAGCTCA GCAGCAGCAG AACTTCACGG GTCTTTCTAC TTTGTCTAAA 
AACATACTCA CTCTAATTGA ACTACCATCT GTTAATACGA TGGAGGAAGA GTATCCTCGG 
ATAGAGAGTG AATCTACAGT ACATATTGGA GTTGGGGAAC CTGACTTCAT CTTGGGACAG 
CTGTATCATC TGTCTTCAGT ACAGGCACCT GAAGTAGCCA AATCTTGGGC CGCATTGGCT 
AGTTGGGCTT ACAGGTGGGG CAGAAAGGTG GTTGACAATG CCAGTCAGGG AGAAGGTGTT 
CGTCTGTTGC CTAGAGAAAA ATCTGAAGTT CAGAATCTGC TCCCAGACAC CATAACCGAA 
GAAGAAAAAG AGAGAATATA TGGTATTCTT GGTCAGGCTG TGTGTCGCCC AGCGGGGATT 
CAGGATGAAG ATATAACGCT TCAGATCACA GAAAGTGAAG ATAATGAGGA AGATGACATG 
GTTGATGTTA TCTGGCGTCA GTTAATATCA AGTTGCCCAT GGCTTTCAGA ACTTGATGAA 
AGTGCAACAG AAGGAGTCAT TAAAGTGTGG AGGAAAGTTG TGGACAGAAT CTTCAGCCTG 
TATAAACTGT CCTGCAGCGC ATATTTTACT TTCCTTAAAC TCAATGCTGG GCAGATTCCT 
TTAGATGAAG ATGACCCTCG GCTCCATTTA AGCCACAGAG TGGAGCAGAG CACGGATGAC 
ATGATTGTGA TGGCCACGCT GCGGTTGCTC AGGTTGCTCG TGAAGCACGC TGGGGAGCTT 
CGGCAGTATC TGGAACATGG CTTGGAAACA ACACCTACTG CGCCTTGGAG AGGAATTATT 
CCGCAGCTTT TCTCACGCTT AAACCACCCT GAAGTGTATG TGCGCCAGAG TATTTGTAAC 
CTCCTCTGCC GCGTGGCTCA GGATTCCCCG CATCTCATAC TGTATCCTGC AATAGTGGGC 
ACCATATCGC TTAGTAGTGA GTCACAGGCT TCAGGAAATA AATTTTCCTC TGCAATTCCA 
ACTTTACTTG GCAATATTCA AGGAGAAGAA CTACTGGTTT CTGAATGTGA GGGAGGAAGT 
CCTCCTGCTT CTCAAGATAG CAATAAGGAT GAACCTAAAA GTGGACTGAA TGAAGACCAA 
GCCATGATGC AGGACTGCTA CAGCAAAATT GTAGATAAAC TGTCCTCGGC AAACCCAACT 
ATGGTGTTAC AGGTTCAGAT GCTTGTGGCT GAGCTGCGCA GGGTCACTGT TCTCTGGGAT 
GAGCTCTGGT TGGGAGTTCT GTTACAGCAG CACATGTATG TCCTGAGACG AATTCAGCAG 
TTGGAAGACG AGGTGAAGAG AGTCCAGAAC AACAACACCT TACGCAAAGA AGAGAAAATC 
GCAATCATGC GGGAGAAGCA CACAGCTTTG ATGAAGCCCA TCGTATTTGC TTTGGAGCAC 
GTTAGGAGCA TCACAGCGGC CCCTGCAGAA ACACCTCATG AAAAGTGGTT TCAGGATAAC 
TATGGTGATG CCATTGAAAA TGCTCTAGAA AAGCTGAAAA CACCGTCAAA CCCTGCAAAG 
CCTGGAAGCA GCTGGATTCC ATTTAAAGAG ATAATGCTGA GTTTGCAACA GAGAGCACAG 
AAACGTGCAA GTTACATCTT GCGTCTTGAA GAAATCAGCC CATGGCTGGC TGCCATGACT 
AACACTGAAA TTGCTCTTCC CGGAGAAGTC TCAGCCAGAG ATACCGTCAC AATCCACAGT 
GTTGGTGGAA CCATCACAAT CTTACCTACT AAAACCAAGC CAAAGAAACT TCTCTTTCTA 
GGATCAGATG GGAAGAGCTA TCCTTATCTT TTCAAAGGAT TGGAGGATTT ACATCTGGAT 
GAGAGAATAA TGCAATTTCT GTCTATTGTA AATACCATGT TTGCAACAAT CAATCGCCAG 
GAAACACCTC GTTTCCATGC TCGCCACTAT TCTGTAACAC CACTGGGAAC TCGCTCAGGC 
CTAATCCAGT GGGTGGATGG AGCCACGCCC TTATTTGGTC TTTACAAACG ATGGCAACAA 
CGGGAAGCCG CCTTGCAAGC ACAAAAGGCC CAAGATTCCT ACCAAACTCC TCAGAATCCT 
GGAATCGTAC CCCGTCCTAG TGAACTTTAT TATAGTAAAA TTGGTCCTGC TTTGAAAGCA 
GTTGGGCTTA GTCTGGATGT GTCCCGCCGG GATTGGCCTC TTCATGTGAT GAAGGCAGTG 
TTAGAAGAGT TAATGGAGGC CACGCCCCCA AATCTCCTTG CTAAAGAGCT TTGGTCATCA 
TGTACCACCC CTGATGAGTG GTGGAGAGTC ACACAGTCTT ATGCCAGATC TACTGCAGTC 
ATGTCTATGG TCGGATACAT AATTGGCCTT GGAGATAGAC ATCTGGATAA TGTTCTCATA 
GATATGACGA CTGGAGAGGT TGTTCACATA GATTACAATG TTTGCTTTGA AAAAGGTAAA 
AGCCTTAGAG TTCCTGAGAA AGTACCTTTT CGAATGACAC AGAACATTGA AACAGCACTG 
GGTGTAACTG GAGTAGAAGG TGTTTTTAGG CTTTCATGTG AGCAGGTTCT ACACATTATG 
CGGCGTGGCA GAGAGACTCT GCTGACGCTG CTGGAGGCCT TTGTATATGA CCCCCTGGTG 
GACTGGACAG CTGGGGGCGA GGCTGGGTTT GCTGGTGCGG TCTATGGTGG AGGGGGCCAG 
CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG ATGGAACGAG AGATTACTCG CAGCCTTTTT 
TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC TGGTTTAAAA ACAGAGATGA GATGCTGGTT 
GTACTTCCCA AGCTGGACAG CAGCTTAGAT GAATACCTGA GCTTGCAAGA GCAACTAACA 
GATGTGGAGA AACTGCAGGG CAAACTGCTG GAAGAAATTG AGTTTCTAGA AGGAGCTGAA 
GGAGTGGATC ATCCTTCTCA TACTCTGCAA CACAGGTATT CTGAACACAC TCAACTACAG 
ACTCAGCAGA GAGCTGTTCA GGAAGCAATC CAGGTGAAGC TGAATGAATT TGAACAGTGG 
ATAACACACT ATCAGGCTGC GTTCAATAAT TTGGAAGCTA CACAGCTGGC CAGTTTGCTT 
CAGGAGATAA GCACACAGAT GGACCTTGGT CCTCCCAGCT ACGTGCCGGC AACAGCCTTT 
CTGCAGAATG CTGGCCAGGC CCACTTGATT AGCCAGTGTG AGCAGCTAGA AGGGGAGGTT 
GGTGCCCTCC TGCAGCAGAG GCGCTCGGTG CTGCGAGGCT GTCTGGAGCA GCTGCATCAC 
TACGCAACTG TTGCTCTGCA GTATCCAAAG GCCATATTTC AGAAACATCG AATTGAGCAG 
TGGAAGACCT GGATGGAAGA GCTCATCTGT AATACCACAG TAGAACGCTG TCAGGAACTT 
TACAGGAAAT ATGAAATGCA GTATGCGCCC CAGCCACCCC CAACAGTGTG TCAGTTCATC 
ACGGCCACTG AGATGACCCT GCAGCGGTAC GCAGCCGACA TCAACAGCCG ACTTATCAGA 
CAGGTGGAAC GCTTGAAACA GGAAGCTGTC ACTGTACCGG TTTGTGAAGA TCAGTTGAAA 
GAAATTGAAC GTTGTATCAA AGTTTTCCTT CACGAGAATG GAGAGGAAGG ATCTTTGAGT 
CTAGCAAGTG TTATTATTTC TGCCCTTTGT ACTCTCACAA GGCGTAACCT AATGATGGAA 
GGTGCAGCGT CGAGTGCTGG AGAGCAGCTG GTTGATCTGA CTTCTCGGGA TGGAGCCTGG 
TTCTTGGAGG AACTCTGCAG TATGAGCGGA AATGTCACAT GTCTGGTTCA GTTACTGAAG 
CAGTGTCATT TAGTGCCACA AGACTTAGAC ATTCCTAACC CCATGGAAGC ATCTGAGGCC 
GTTCATTTGG CTAATGGTGT ATACACCTCA CTTCAGGAAT TAAATTCAAA TTTCCGGCAA 
ATCATATTTC CAGAAGCACT GAGATGTTTA ATGAAAGGAG AATACACGTT AGAAAATATG 
CTTCATGAGC TGGACAGTCT TATTGAGCAG ACAACTGATG GTGTTCCTCT GCAGACTCTA 
GTTGAATCTC TTCAAGCCTA TTTAAGAAAT GCAGCTATGG GACTTGAGGA AGAAACACAT 
GCTCATTACA TCGATGTTGC CAGAATGCTT CATGCTCAGT ATGGTGAATT AATCCAACCA 
AGAAATGGTT CAGTTGATGA AACACCAAAA ATGTCAGCTG GCCAGATGCT TTTGGTAGCA 
TTTGATGGCA TGTTTGCCCA AGTTGAGACT GCTTTTGGCT TATTGGTTGA AAAGCTAAAT 
AAGATGGAAA TTCCTATAGC TTGGCGAAAG ATTGACATAA TACGGGAAGC CAGGAGCACT 
CAGGTCAATT TTTTTGATGA TGATAATCAC CGACAGGTGC TGGAAGAGAT CTTCTTTCTA 
AAACGACTAC AGACAATTAA GGAGTTCTTT CGGCTCTGTG GTACCTTTTC TAAAACTTTG 
TCAGGATCAA GTTCACTTGA AGATCAGAAT ACTGTAAATG GGCCTGTACA GATTGTCAAT 
GTGAAAACCC TTTTTAGAAA CTCTTGTTTC AGTGAAGACC AGATGGCCAA ACCAATAAAG 
GCATTCACAG CTGACTTTGT GCGGCAGCTC CTGATTGGAC TCCCGAACCA AGCCCTGGGC 
CTCACACTGT GCAGCTTCAT CAGTGCTCTA GGGGTAGACA TCATTGCGCA GGTGGAGGCG 
AAGGATTTTG GTGCTGAAAG CAAAGTTTCT GTTGACGATC TGTGTAAGAA GGCGGTGGAG 
CACAACATCC AGATCGGGAA GTTCTCTCAG CTGGTCATGA ACAGGGCGAC TGTGCTGGCG 
AGCTCCTACG ACACTGCCTG GAAGAAGCAC GACTTGGTGC GCAGGCTGGA GACCAGCATC 
GCCTCCTGCA AGACAAGCCT GCAGCGTGTC CAGCTGCACA TCGCCATGTT TCAGTGGCAG 
CATGAAGATC TCCTTATCAA TAGACCACAA GCCATGTCTG TCACACCTCC CCCTCGTTCT 
GCCATATTAA CCAGCATGAA AAAGAAACTC CATACTCTGA GCCAGATTGA AACTTCAATT 
GCAACAGTTC AGGAGAAACT GGCAGCACTT GAAGCAAGTA TTGAACAGCG ACTCAAATGG 
GCAGGTGGTG CCAACCCTGC ACTGGCACCT GTACTACAGG ATTTTGAAGC AACAATAGCT 
GAAAGAAGAA ATCTTGTCCT TAAGGAGAGC CAAAGAGCAA GTCAGGTCAC TTTCCTCTGC 
AGCAATATCA TTCATTTTGA AAGTTTACGA ACTAGAACTG CAGAAGCCTT AAACCTGGAT 
GCTGCATTAT TTGAACTAAT CAAACGGTGT CAGCAAATGT GTTCATTTGC ATCGCAGTTT 
AACAGTTCAG TCTCTGAGTT AGAGCTTCGT TTATTACAGA GGGTGGACAC GACTCTTGAA 
CATCCTATTG GCAGCTCTGA GTGGCTTTTG TCAGCACACA AACAACTGAC CCAGGATATG 
TCTACTCAGA GAGCAATTCA GACAGAGAAA GAGCAACAAA TAGAAACAGT CTGTGAAACA 
ATTCAGAATC TGGTTGATAA TATAAAGACT GTGCTCACTG GCCATAACCG GCAGCTTGGA 
GATGTCAAAC ATCTCCTGAA AGCAATGGCC AAGGATGAAG AAGCTGCTCT GGCAGATGGT 
GAAGATGTTC CCTATGAGAA CAGTGTTAGG CAGTTCTTGG GTGAATATAA ATCATGGCAA 
GACAACATTC AGACAGTGCT GTTTACATTA GTCCAAGCCA TGGGTCAGGT TCGAAGTCAA 
GAACATGTTG AAATGCTCCA GGAAATAACT CCCACCTTGA AAGAACTGAA AACGCAAAGT 
CAGAGTGTGT ATAATAATTT AGTGAGTTTT GCATCACCAT TAGTGACCGA TGCAACAAAT 
GAATGTTCGA GTCCAACATC GTCTGCTACT TATCAACCAT CCTTTGCTGC AGCAGTCCGA 
AGCAACACTG GCCAGAAGAC TCAGCCTGAT GTCATGTCAC AGAACGCTAG AAAGCTGATT 
CAGAAAAACC TAGCTACATC AGCAGATACC CCACCGAGCA CCGTTCCGGG AACTGGCAAG 
AGTGTTGCTT GTAGTCCTAA AAAGGCCATC AGAGACCCTA AGACTGGAAA AGCTGTGCAG 
GAGAGAAACT CGTATGCGGT GAGTGTGTGG AAGAGAGTGA AAGCCAAGCT AGAGGGCCGA 
GATGTTGATC CGAATAGGAG GATGTCAGTT GCTGAACAGG TTGACTATGT CATTAAGGAA 
GCAACTAATC TAGATAACTT GGCTCAGCTG TATGAAGGTT GGACAGCCTG GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192844.1
CDS	1..10977
Translation	MSRRAPGSRLSSGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRIHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSIQHGSRALAIKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRIGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNPSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSVVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANTGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFIECTEQLELLPGENISLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSITENVVKYLKQTSRIAVGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLATEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQSQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEHAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLENMLHELDSLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSIASCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	NM_001205915.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205915.1

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATAATTTA 
AAATACTCTT CATCCAGAGA TAGGGGTGGG TCTTCCTCTT ATGGACTGCA GCCTTCAAAT 
TCAGCTGTGG TGTCTCGGCA AAGGCATGAT GATACCAGAA TCCATGCTGA CATACAGAAT 
GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG 
TCGTTGGGGC AAGAGCTGAG GGTTAACAAT GTGACCAGCC CTGAGTTTAC CAGTATTCAG 
CATGGCAGTC GTGCGTTAGC CATCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCT 
TATTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA 
GACCGAAGGC TGGCTACTGT TAAGCAGTTG AAAGAATTTA TTCAGCAACC AGAAAATAAG 
CTGGTGCTAG TTAAGCAGTT GGATAATATC TTGGCTGCTG TACATGATGT GCTTAATGAA 
AGTAGCAAAT TGCTTCAGGA GTTGAGACAG GAGGGAGCTT GTTGTCTTGG CCTTCTTTGT 
GCATCTCTGA GCTATGAGGC TGAGAAGATC TTCAAATGGA TTTTTAGCAA ATTTAGCTCA 
TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC TTATGTGCCA CCTACAAAGC ACTAGAGACT 
GTAGGAGAAA AGAAAGCCTT TTCATCTGTA ATGCAGCTTG TAATGACCAG CCTGCAGTCC 
ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAGT GTGTTAAGTG CATTCTTTTG 
GTAGCTCGAT GTTACCCTCA TATTTTCAGC ACAAATTTTA GGGATACAGT TGATATATTA 
GTTGGATGGC ATATAGATCA TACTCAGAAG CCTTCACTTA CACAACAGGT GTCTGGGTGG 
TTGCAGAGTT TGGAGCCATT CTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT 
CAGTTTTTAG AGGACATGGA GGCATATGCT GAGGACCTCA GCCATGTAGC CTCTGGGGAG 
TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCCTTAC CAAAGCTGGC AGCACTTCTC 
CGGGTATTCA GTACTGTGGT GAGAAGCATT GGGGAACGTT TCAGCCCAAT TCGAGGTCCT 
CCAATTACTG AGGCATATGT AACAGATGTT CTGTACAGAG TAATGAGATG TGTGACGGCT 
GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT 
TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTTAT TACATATGGA 
TTAGATCAAC TGGAGAATTG CCAGACTTGT GGTACAGATT ATATCATCTC AGTCTTGAAT 
TTACTCACAT TGATTGTTGA ACAGATAAAT ACAAAACTGC CATCATCATT TGTAGAAAAA 
CTGTTTATAC CATCATCTAA GCTACTATTT TTGCGTTATC ATAAAGAAAA AGAGGTTGTT 
GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG CTCAGTCTGA AGAATATTCC TGTTTTAGAG 
ACTGCCTATA AATTAATTTT GGGAGAAATG ACTTGTGCCC TAAACAATCT GCTACATAGT 
CTACAACTTC CTGATGCCTG TTCTGAAATA AAACATGAGG CTTTTAAGAA TCATGTTTTC 
AATGTAGACA ATGCAAAATT TGTAGTTATA TTTGACCTTA GTGCTCTGAC TACAATTGGA 
AATGCTAAAA ACTCACTAAT TGGGATGTGG GCCCTGTCTC CAACTGTGTT TGCACTTCTG 
AGTAAGAATC TGATGATTGT GCATGGCGAC CTGGCTGTTC ATTTCCCTGC CATTCAGTAT 
GCAGTGCTCT ACACATTGTA TTCTCATTGT ACCAGGCATG ACCACTTTAT ATCTAGTAGT 
CTTAGTTCAT CATCCCCGTC CTTGTTTGAT GGTGCCGTTA TTAGTACTGT AACTACAGCT 
ACAAAAAAAC ATTTCTCAAT TATCTTAAAT CTCCTGGGAA TATTGCTTAA GAAAGATAAT 
CTTAACCAGG ACACAAGGAA ACTGTTAATG ACCTGGGCTT TGGAAGTGGC TGTTTTAATG 
AAGAAGTCTG AAACATATGC ACCTTTATTC TCTCTTCCAT CTTTCCATAA ATTTTGCAAA 
GGCCTTTTAG CCAACACTCT GGTCGAAGAT GTGAATATCT GTCTGCAAGC ATGCAGCAGT 
CTACATGCAC TGTCCTCTTC CTTACCAGAT GATCTTTTAC AGAGGTGTGT TGATGTTTGC 
CGTGTTCAGC TAGTCCATAG TGGAACTCGT ATTCGACAAG CATTTGGAAA GCTATTGAAG 
TCAATCCCTT TAGATGTTGT CTTGAGCAAT AACAATCACA CAGAAATTCA AGAAATTTCT 
TTAGCATTAA GAAGTCACAT GAGTAAAGCA CCAAGTAACA CATTCCACCC ACAAGATTTC 
TCTGATGTTA TTAGTTTTAT TTTGTATGGA AACTCTCATA GAATAGGGAA GGACAATTGG 
TTAGAAAGAT TGTTCTATAG CTGCCAGAGA CTGGATAAAC GTGACCAATC AACGATTCCC 
CGCAATCTGC TAAAGACAGA TGCTGTCCTC TGGCAGTGGG CTATTTGGGA AGCAGCACAG 
TTCACTGTCC TTTCTAAGCT GAGGACCCCA CTTGGCAGAG CTCAGGATAC CTTCCAAACA 
ATTGAAGGTA TCATTAGAAG TCTTGCAGCT CACACATTAA ACCCCGATCA AGATGTTAGT 
CAGTGGACAA CTGCAGACAA TGACGAAGGC CACAGTAGCA ACCAGCTTAG ACTTGTGCTG 
CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG TTAATGTATA ATGCATATGA AGGATGTGCC 
AATGCATTAA CATCGCCTCC CAAGGTCATT AGGACTTTTT TCTATACCAA TCGCCAAACT 
TGTCAAGACT GGCTGACTCG GATCCGACTC TCCATCATGA GGGTTGGATT GTTGGCAGGC 
CAGCCTGCTG TGACAGTGAG ACACGGCTTC GATTTGCTTA CAGAAATGAA GACAAATCCG 
TCTCAGGGAA ATGAATTGGA AGTAACTATC ATGATGGTGG TAGAAGCATT GTGTGAACTT 
CATTGTCCTG AAGCTATACA GGGAATTGCT GTCTGGTCGT CTTCGGTTGT TGGAAAAAAT 
CTTCTCTGGA TTAATTCAGT GGCCCAACAG GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG 
GAGTACCAGG AGCACCTGTG TGCCATGACA GGTGTCGATT GCTGCATCTC TAGCTTCGAC 
AAATCCGTTC TCACATTGGC TAATACTGGG CGTAATAGTG CCAGCCCCAA GCATTCTTTG 
AATGGTGAAT CCAGAAAAAC TGTGCTTTCC AAACCAACTG ATTCTTCTCC TGAGGTTATA 
AATTATTTGG GAAATAAAGC ATGTGAGTGC TACATTTCAA TAGCTGATTG GGCTGCTGTA 
CAGGAGTGGC AGAATGCTGT CCATGACTTG AAAAAGAGTA CCAGTAGTAC TTCTCTCAAC 
CTAAAGGCTG ATTTCAACTA CATAAAATCA CTAAGCAGTT TTGAATCTGG AGAATTTATT 
GAATGCACTG AACAATTAGA ATTGTTACCA GGAGAAAATA TCAGTCTACT TGCTGGAGGA 
TCAAAAGAAA AAATAGATAT GAAAAAACTG CTTCCTAACA TGTTAAGTCC AGATCCAAGA 
GAACTTCAGA AATCCATTGA AGTTCAGTTG TTAAGAAGTT CTGTTTGTTT GGCAACTGCT 
TTAAACCACA TAGAACAAGA TCAGAAGTGG CAGTCTATTA CCGAAAATGT GGTGAAGTAC 
CTGAAGCAAA CCTCCCGCAT AGCTGTTGGA CCCCTGAGAC TTTCTACTCT AACAGTTTCC 
CAGTCTCTGC CAGTCCTAAG TACTTTACAG CTGTATTGCT CTTCTGCTTT GGAGAACACA 
GTTTCCAACA GACTTGCCAC AGAGGACTGT CTTATTCCAC TCTTTAGTGA AGCTTTGCGT 
TCATGTAAAC AGCATGATGT CAGGCCATGG ATGCAAGCAT TAAGATACAC GATGTACCAG 
AGTCAGTTGT TGGAGAAAAT AAAAGAACAA ACAGTTCCAA TTAGAAGCCA TCTCATGGAA 
TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA AAACGGGGGA ATGTGTCACT TGCAACAAGA 
TTGCTGGCAC AGTGTAGCGA GGTTCAGCTG GGAAAGACTA CCACTGCACA GGATTTAGTC 
CAGCACTTTA AAAAACTATC AACTCAAGGT CAAGTGGATG AAAAATGGGG GCCTGAACTT 
GACATTGAAA AAACGAAACT GCTATATACA GCAGGCCAGT CAACACATGC AATGGAGATG 
TTGAGTTCCT GTGCCATCTC TTTCTGCAAG TCTGCCAAAG CTGAGCATGC TGTTGCCAAG 
TCAATTTTGA CATTGGCTAA ATGGATCCAG GCAGAATGGA AAGAAATCTC AGGACAGCTG 
AAACAGGTTT ACAGAGCTCA GCAGCAGCAG AACTTCACGG GTCTTTCTAC TTTGTCTAAA 
AACATACTCA CTCTAATTGA ACTACCATCT GTTAATACGA TGGAGGAAGA GTATCCTCGG 
ATAGAGAGTG AATCTACAGT ACATATTGGA GTTGGGGAAC CTGACTTCAT CTTGGGACAG 
CTGTATCATC TGTCTTCAGT ACAGGCACCT GAAGTAGCCA AATCTTGGGC CGCATTGGCT 
AGTTGGGCTT ACAGGTGGGG CAGAAAGGTG GTTGACAATG CCAGTCAGGG AGAAGGTGTT 
CGTCTGTTGC CTAGAGAAAA ATCTGAAGTT CAGAATCTGC TCCCAGACAC CATAACCGAA 
GAAGAAAAAG AGAGAATATA TGGTATTCTT GGTCAGGCTG TGTGTCGCCC AGCGGGGATT 
CAGGATGAAG ATATAACGCT TCAGATCACA GAAAGTGAAG ATAATGAGGA AGATGACATG 
GTTGATGTTA TCTGGCGTCA GTTAATATCA AGTTGCCCAT GGCTTTCAGA ACTTGATGAA 
AGTGCAACAG AAGGAGTCAT TAAAGTGTGG AGGAAAGTTG TGGACAGAAT CTTCAGCCTG 
TATAAACTGT CCTGCAGCGC ATATTTTACT TTCCTTAAAC TCAATGCTGG GCAGATTCCT 
TTAGATGAAG ATGACCCTCG GCTCCATTTA AGCCACAGAG TGGAGCAGAG CACGGATGAC 
ATGATTGTGA TGGCCACGCT GCGGTTGCTC AGGTTGCTCG TGAAGCACGC TGGGGAGCTT 
CGGCAGTATC TGGAACATGG CTTGGAAACA ACACCTACTG CGCCTTGGAG AGGAATTATT 
CCGCAGCTTT TCTCACGCTT AAACCACCCT GAAGTGTATG TGCGCCAGAG TATTTGTAAC 
CTCCTCTGCC GCGTGGCTCA GGATTCCCCG CATCTCATAC TGTATCCTGC AATAGTGGGC 
ACCATATCGC TTAGTAGTGA GTCACAGGCT TCAGGAAATA AATTTTCCTC TGCAATTCCA 
ACTTTACTTG GCAATATTCA AGGAGAAGAA CTACTGGTTT CTGAATGTGA GGGAGGAAGT 
CCTCCTGCTT CTCAAGATAG CAATAAGGAT GAACCTAAAA GTGGACTGAA TGAAGACCAA 
GCCATGATGC AGGACTGCTA CAGCAAAATT GTAGATAAAC TGTCCTCGGC AAACCCAACT 
ATGGTGTTAC AGGTTCAGAT GCTTGTGGCT GAGCTGCGCA GGGTCACTGT TCTCTGGGAT 
GAGCTCTGGT TGGGAGTTCT GTTACAGCAG CACATGTATG TCCTGAGACG AATTCAGCAG 
TTGGAAGACG AGGTGAAGAG AGTCCAGAAC AACAACACCT TACGCAAAGA AGAGAAAATC 
GCAATCATGC GGGAGAAGCA CACAGCTTTG ATGAAGCCCA TCGTATTTGC TTTGGAGCAC 
GTTAGGAGCA TCACAGCGGC CCCTGCAGAA ACACCTCATG AAAAGTGGTT TCAGGATAAC 
TATGGTGATG CCATTGAAAA TGCTCTAGAA AAGCTGAAAA CACCGTCAAA CCCTGCAAAG 
CCTGGAAGCA GCTGGATTCC ATTTAAAGAG ATAATGCTGA GTTTGCAACA GAGAGCACAG 
AAACGTGCAA GTTACATCTT GCGTCTTGAA GAAATCAGCC CATGGCTGGC TGCCATGACT 
AACACTGAAA TTGCTCTTCC CGGAGAAGTC TCAGCCAGAG ATACCGTCAC AATCCACAGT 
GTTGGTGGAA CCATCACAAT CTTACCTACT AAAACCAAGC CAAAGAAACT TCTCTTTCTA 
GGATCAGATG GGAAGAGCTA TCCTTATCTT TTCAAAGGAT TGGAGGATTT ACATCTGGAT 
GAGAGAATAA TGCAATTTCT GTCTATTGTA AATACCATGT TTGCAACAAT CAATCGCCAG 
GAAACACCTC GTTTCCATGC TCGCCACTAT TCTGTAACAC CACTGGGAAC TCGCTCAGGC 
CTAATCCAGT GGGTGGATGG AGCCACGCCC TTATTTGGTC TTTACAAACG ATGGCAACAA 
CGGGAAGCCG CCTTGCAAGC ACAAAAGGCC CAAGATTCCT ACCAAACTCC TCAGAATCCT 
GGAATCGTAC CCCGTCCTAG TGAACTTTAT TATAGTAAAA TTGGTCCTGC TTTGAAAGCA 
GTTGGGCTTA GTCTGGATGT GTCCCGCCGG GATTGGCCTC TTCATGTGAT GAAGGCAGTG 
TTAGAAGAGT TAATGGAGGC CACGCCCCCA AATCTCCTTG CTAAAGAGCT TTGGTCATCA 
TGTACCACCC CTGATGAGTG GTGGAGAGTC ACACAGTCTT ATGCCAGATC TACTGCAGTC 
ATGTCTATGG TCGGATACAT AATTGGCCTT GGAGATAGAC ATCTGGATAA TGTTCTCATA 
GATATGACGA CTGGAGAGGT TGTTCACATA GATTACAATG TTTGCTTTGA AAAAGGTAAA 
AGCCTTAGAG TTCCTGAGAA AGTACCTTTT CGAATGACAC AGAACATTGA AACAGCACTG 
GGTGTAACTG GAGTAGAAGG TGTTTTTAGG CTTTCATGTG AGCAGGTTCT ACACATTATG 
CGGCGTGGCA GAGAGACTCT GCTGACGCTG CTGGAGGCCT TTGTATATGA CCCCCTGGTG 
GACTGGACAG CTGGGGGCGA GGCTGGGTTT GCTGGTGCGG TCTATGGTGG AGGGGGCCAG 
CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG ATGGAACGAG AGATTACTCG CAGCCTTTTT 
TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC TGGTTTAAAA ACAGAGATGA GATGCTGGTT 
GTACTTCCCA AGCTGGACAG CAGCTTAGAT GAATACCTGA GCTTGCAAGA GCAACTAACA 
GATGTGGAGA AACTGCAGGG CAAACTGCTG GAAGAAATTG AGTTTCTAGA AGGAGCTGAA 
GGAGTGGATC ATCCTTCTCA TACTCTGCAA CACAGGTATT CTGAACACAC TCAACTACAG 
ACTCAGCAGA GAGCTGTTCA GGAAGCAATC CAGGTGAAGC TGAATGAATT TGAACAGTGG 
ATAACACACT ATCAGGCTGC GTTCAATAAT TTGGAAGCTA CACAGCTGGC CAGTTTGCTT 
CAGGAGATAA GCACACAGAT GGACCTTGGT CCTCCCAGCT ACGTGCCGGC AACAGCCTTT 
CTGCAGAATG CTGGCCAGGC CCACTTGATT AGCCAGTGTG AGCAGCTAGA AGGGGAGGTT 
GGTGCCCTCC TGCAGCAGAG GCGCTCGGTG CTGCGAGGCT GTCTGGAGCA GCTGCATCAC 
TACGCAACTG TTGCTCTGCA GTATCCAAAG GCCATATTTC AGAAACATCG AATTGAGCAG 
TGGAAGACCT GGATGGAAGA GCTCATCTGT AATACCACAG TAGAACGCTG TCAGGAACTT 
TACAGGAAAT ATGAAATGCA GTATGCGCCC CAGCCACCCC CAACAGTGTG TCAGTTCATC 
ACGGCCACTG AGATGACCCT GCAGCGGTAC GCAGCCGACA TCAACAGCCG ACTTATCAGA 
CAGGTGGAAC GCTTGAAACA GGAAGCTGTC ACTGTACCGG TTTGTGAAGA TCAGTTGAAA 
GAAATTGAAC GTTGTATCAA AGTTTTCCTT CACGAGAATG GAGAGGAAGG ATCTTTGAGT 
CTAGCAAGTG TTATTATTTC TGCCCTTTGT ACTCTCACAA GGCGTAACCT AATGATGGAA 
GGTGCAGCGT CGAGTGCTGG AGAGCAGCTG GTTGATCTGA CTTCTCGGGA TGGAGCCTGG 
TTCTTGGAGG AACTCTGCAG TATGAGCGGA AATGTCACAT GTCTGGTTCA GTTACTGAAG 
CAGTGTCATT TAGTGCCACA AGACTTAGAC ATTCCTAACC CCATGGAAGC ATCTGAGGCC 
GTTCATTTGG CTAATGGTGT ATACACCTCA CTTCAGGAAT TAAATTCAAA TTTCCGGCAA 
ATCATATTTC CAGAAGCACT GAGATGTTTA ATGAAAGGAG AATACACGTT AGAAAATATG 
CTTCATGAGC TGGACAGTCT TATTGAGCAG ACAACTGATG GTGTTCCTCT GCAGACTCTA 
GTTGAATCTC TTCAAGCCTA TTTAAGAAAT GCAGCTATGG GACTTGAGGA AGAAACACAT 
GCTCATTACA TCGATGTTGC CAGAATGCTT CATGCTCAGT ATGGTGAATT AATCCAACCA 
AGAAATGGTT CAGTTGATGA AACACCAAAA ATGTCAGCTG GCCAGATGCT TTTGGTAGCA 
TTTGATGGCA TGTTTGCCCA AGTTGAGACT GCTTTTGGCT TATTGGTTGA AAAGCTAAAT 
AAGATGGAAA TTCCTATAGC TTGGCGAAAG ATTGACATAA TACGGGAAGC CAGGAGCACT 
CAGGTCAATT TTTTTGATGA TGATAATCAC CGACAGGTGC TGGAAGAGAT CTTCTTTCTA 
AAACGACTAC AGACAATTAA GGAGTTCTTT CGGCTCTGTG GTACCTTTTC TAAAACTTTG 
TCAGGATCAA GTTCACTTGA AGATCAGAAT ACTGTAAATG GGCCTGTACA GATTGTCAAT 
GTGAAAACCC TTTTTAGAAA CTCTTGTTTC AGTGAAGACC AGATGGCCAA ACCAATAAAG 
GCATTCACAG CTGACTTTGT GCGGCAGCTC CTGATTGGAC TCCCGAACCA AGCCCTGGGC 
CTCACACTGT GCAGCTTCAT CAGTGCTCTA GGGGTAGACA TCATTGCGCA GGTGGAGGCG 
AAGGATTTTG GTGCTGAAAG CAAAGTTTCT GTTGACGATC TGTGTAAGAA GGCGGTGGAG 
CACAACATCC AGATCGGGAA GTTCTCTCAG CTGGTCATGA ACAGGGCGAC TGTGCTGGCG 
AGCTCCTACG ACACTGCCTG GAAGAAGCAC GACTTGGTGC GCAGGCTGGA GACCAGCATC 
GCCTCCTGCA AGACAAGCCT GCAGCGTGTC CAGCTGCACA TCGCCATGTT TCAGTGGCAG 
CATGAAGATC TCCTTATCAA TAGACCACAA GCCATGTCTG TCACACCTCC CCCTCGTTCT 
GCCATATTAA CCAGCATGAA AAAGAAACTC CATACTCTGA GCCAGATTGA AACTTCAATT 
GCAACAGTTC AGGAGAAACT GGCAGCACTT GAAGCAAGTA TTGAACAGCG ACTCAAATGG 
GCAGGTGGTG CCAACCCTGC ACTGGCACCT GTACTACAGG ATTTTGAAGC AACAATAGCT 
GAAAGAAGAA ATCTTGTCCT TAAGGAGAGC CAAAGAGCAA GTCAGGTCAC TTTCCTCTGC 
AGCAATATCA TTCATTTTGA AAGTTTACGA ACTAGAACTG CAGAAGCCTT AAACCTGGAT 
GCTGCATTAT TTGAACTAAT CAAACGGTGT CAGCAAATGT GTTCATTTGC ATCGCAGTTT 
AACAGTTCAG TCTCTGAGTT AGAGCTTCGT TTATTACAGA GGGTGGACAC GACTCTTGAA 
CATCCTATTG GCAGCTCTGA GTGGCTTTTG TCAGCACACA AACAACTGAC CCAGGATATG 
TCTACTCAGA GAGCAATTCA GACAGAGAAA GAGCAACAAA TAGAAACAGT CTGTGAAACA 
ATTCAGAATC TGGTTGATAA TATAAAGACT GTGCTCACTG GCCATAACCG GCAGCTTGGA 
GATGTCAAAC ATCTCCTGAA AGCAATGGCC AAGGATGAAG AAGCTGCTCT GGCAGATGGT 
GAAGATGTTC CCTATGAGAA CAGTGTTAGG CAGTTCTTGG GTGAATATAA ATCATGGCAA 
GACAACATTC AGACAGTGCT GTTTACATTA GTCCAAGCCA TGGGTCAGGT TCGAAGTCAA 
GAACATGTTG AAATGCTCCA GGAAATAACT CCCACCTTGA AAGAACTGAA AACGCAAAGT 
CAGAGTGTGT ATAATAATTT AGTGAGTTTT GCATCACCAT TAGTGACCGA TGCAACAAAT 
GAATGTTCGA GTCCAACATC GTCTGCTACT TATCAACCAT CCTTTGCTGC AGCAGTCCGA 
AGCAACACTG GCCAGAAGAC TCAGCCTGAT GTCATGTCAC AGAACGCTAG AAAGCTGATT 
CAGAAAAACC TAGCTACATC AGCAGATACC CCACCGAGCA CCGTTCCGGG AACTGGCAAG 
AGTGTTGCTT GTAGTCCTAA AAAGGCCATC AGAGACCCTA AGACTGGAAA AGCTGTGCAG 
GAGAGAAACT CGTATGCGGT GAGTGTGTGG AAGAGAGTGA AAGCCAAGCT AGAGGGCCGA 
GATGTTGATC CGAATAGGAG GATGTCAGTT GCTGAACAGG TTGACTATGT CATTAAGGAA 
GCAACTAATC TAGATAACTT GGCTCAGCTG TATGAAGGTT GGACAGCCTG GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_024984919.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840687.1 Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

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CloneID	OBa230306
Clone ID Related Accession (Same CDS sequence)	XM_024984919.1
Accession Version	XM_024984919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATAATTTA 
AAATACTCTT CATCCAGAGA TAGGGGTGGG TCTTCCTCTT ATGGACTGCA GCCTTCAAAT 
TCAGCTGTGG TGTCTCGGCA AAGGCATGAT GATACCAGAA TCCATGCTGA CATACAGAAT 
GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG 
TCGTTGGGGC AAGAGCTGAG GGTTAACAAT GTGACCAGCC CTGAGTTTAC CAGTATTCAG 
CATGGCAGTC GTGCGTTAGC CATCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCT 
TATTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA 
GACCGAAGGC TGGCTACTGT TAAGCAGTTG AAAGAATTTA TTCAGCAACC AGAAAATAAG 
CTGGTGCTAG TTAAGCAGTT GGATAATATC TTGGCTGCTG TACATGATGT GCTTAATGAA 
AGTAGCAAAT TGCTTCAGGA GTTGAGACAG GAGGGAGCTT GTTGTCTTGG CCTTCTTTGT 
GCATCTCTGA GCTATGAGGC TGAGAAGATC TTCAAATGGA TTTTTAGCAA ATTTAGCTCA 
TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC TTATGTGCCA CCTACAAAGC ACTAGAGACT 
GTAGGAGAAA AGAAAGCCTT TTCATCTGTA ATGCAGCTTG TAATGACCAG CCTGCAGTCC 
ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAGT GTGTTAAGTG CATTCTTTTG 
GTAGCTCGAT GTTACCCTCA TATTTTCAGC ACAAATTTTA GGGATACAGT TGATATATTA 
GTTGGATGGC ATATAGATCA TACTCAGAAG CCTTCACTTA CACAACAGGT GTCTGGGTGG 
TTGCAGAGTT TGGAGCCATT CTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT 
CAGTTTTTAG AGGACATGGA GGCATATGCT GAGGACCTCA GCCATGTAGC CTCTGGGGAG 
TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCCTTAC CAAAGCTGGC AGCACTTCTC 
CGGGTATTCA GTACTGTGGT GAGAAGCATT GGGGAACGTT TCAGCCCAAT TCGAGGTCCT 
CCAATTACTG AGGCATATGT AACAGATGTT CTGTACAGAG TAATGAGATG TGTGACGGCT 
GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT 
TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTTAT TACATATGGA 
TTAGATCAAC TGGAGAATTG CCAGACTTGT GGTACAGATT ATATCATCTC AGTCTTGAAT 
TTACTCACAT TGATTGTTGA ACAGATAAAT ACAAAACTGC CATCATCATT TGTAGAAAAA 
CTGTTTATAC CATCATCTAA GCTACTATTT TTGCGTTATC ATAAAGAAAA AGAGATGTGG 
GCCCTGTCTC CAACTGTGTT TGCACTTCTG AGTAAGAATC TGATGATTGT GCATGGCGAC 
CTGGCTGTTC ATTTCCCTGC CATTCAGTAT GCAGTGCTCT ACACATTGTA TTCTCATTGT 
ACCAGGCATG ACCACTTTAT ATCTAGTAGT CTTAGTTCAT CATCCCCGTC CTTGTTTGAT 
GGTGCCGTTA TTAGTACTGT AACTACAGCT ACAAAAAAAC ATTTCTCAAT TATCTTAAAT 
CTCCTGGGAA TATTGCTTAA GAAAGATAAT CTTAACCAGG ACACAAGGAA ACTGTTAATG 
ACCTGGGCTT TGGAAGTGGC TGTTTTAATG AAGAAGTCTG AAACATATGC ACCTTTATTC 
TCTCTTCCAT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT GGTCGAAGAT 
GTGAATATCT GTCTGCAAGC ATGCAGCAGT CTACATGCAC TGTCCTCTTC CTTACCAGAT 
GATCTTTTAC AGAGGTGTGT TGATGTTTGC CGTGTTCAGC TAGTCCATAG TGGAACTCGT 
ATTCGACAAG CATTTGGAAA GCTATTGAAG TCAATCCCTT TAGATGTTGT CTTGAGCAAT 
AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA 
CCAAGTAACA CATTCCACCC ACAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGA 
AACTCTCATA GAATAGGGAA GGACAATTGG TTAGAAAGAT TGTTCTATAG CTGCCAGAGA 
CTGGATAAAC GTGACCAATC AACGATTCCC CGCAATCTGC TAAAGACAGA TGCTGTCCTC 
TGGCAGTGGG CTATTTGGGA AGCAGCACAG TTCACTGTCC TTTCTAAGCT GAGGACCCCA 
CTTGGCAGAG CTCAGGATAC CTTCCAAACA ATTGAAGGTA TCATTAGAAG TCTTGCAGCT 
CACACATTAA ACCCCGATCA AGATGTTAGT CAGTGGACAA CTGCAGACAA TGACGAAGGC 
CACAGTAGCA ACCAGCTTAG ACTTGTGCTG CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG 
TTAATGTATA ATGCATATGA AGGATGTGCC AATGCATTAA CATCGCCTCC CAAGGTCATT 
AGGACTTTTT TCTATACCAA TCGCCAAACT TGTCAAGACT GGCTGACTCG GATCCGACTC 
TCCATCATGA GGGTTGGATT GTTGGCAGGC CAGCCTGCTG TGACAGTGAG ACACGGCTTC 
GATTTGCTTA CAGAAATGAA GACAAATCCG TCTCAGGGAA ATGAATTGGA AGTAACTATC 
ATGATGGTGG TAGAAGCATT GTGTGAACTT CATTGTCCTG AAGCTATACA GGGAATTGCT 
GTCTGGTCGT CTTCGGTTGT TGGAAAAAAT CTTCTCTGGA TTAATTCAGT GGCCCAACAG 
GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG GAGTACCAGG AGCACCTGTG TGCCATGACA 
GGTGTCGATT GCTGCATCTC TAGCTTCGAC AAATCCGTTC TCACATTGGC TAATACTGGG 
CGTAATAGTG CCAGCCCCAA GCATTCTTTG AATGGTGAAT CCAGAAAAAC TGTGCTTTCC 
AAACCAACTG ATTCTTCTCC TGAGGTTATA AATTATTTGG GAAATAAAGC ATGTGAGTGC 
TACATTTCAA TAGCTGATTG GGCTGCTGTA CAGGAGTGGC AGAATGCTGT CCATGACTTG 
AAAAAGAGTA CCAGTAGTAC TTCTCTCAAC CTAAAGGCTG ATTTCAACTA CATAAAATCA 
CTAAGCAGTT TTGAATCTGG AGAATTTATT GAATGCACTG AACAATTAGA ATTGTTACCA 
GGAGAAAATA TCAGTCTACT TGCTGGAGGA TCAAAAGAAA AAATAGATAT GAAAAAACTG 
CTTCCTAACA TGTTAAGTCC AGATCCAAGA GAACTTCAGA AATCCATTGA AGTTCAGTTG 
TTAAGAAGTT CTGTTTGTTT GGCAACTGCT TTAAACCACA TAGAACAAGA TCAGAAGTGG 
CAGTCTATTA CCGAAAATGT GGTGAAGTAC CTGAAGCAAA CCTCCCGCAT AGCTGTTGGA 
CCCCTGAGAC TTTCTACTCT AACAGTTTCC CAGTCTCTGC CAGTCCTAAG TACTTTACAG 
CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCCAACA GACTTGCCAC AGAGGACTGT 
CTTATTCCAC TCTTTAGTGA AGCTTTGCGT TCATGTAAAC AGCATGATGT CAGGCCATGG 
ATGCAAGCAT TAAGATACAC GATGTACCAG AGTCAGTTGT TGGAGAAAAT AAAAGAACAA 
ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA 
AAACGGGGGA ATGTGTCACT TGCAACAAGA TTGCTGGCAC AGTGTAGCGA GGTTCAGCTG 
GGAAAGACTA CCACTGCACA GGATTTAGTC CAGCACTTTA AAAAACTATC AACTCAAGGT 
CAAGTGGATG AAAAATGGGG GCCTGAACTT GACATTGAAA AAACGAAACT GCTATATACA 
GCAGGCCAGT CAACACATGC AATGGAGATG TTGAGTTCCT GTGCCATCTC TTTCTGCAAG 
TCTGCCAAAG CTGAGCATGC TGTTGCCAAG TCAATTTTGA CATTGGCTAA ATGGATCCAG 
GCAGAATGGA AAGAAATCTC AGGACAGCTG AAACAGGTTT ACAGAGCTCA GCAGCAGCAG 
AACTTCACGG GTCTTTCTAC TTTGTCTAAA AACATACTCA CTCTAATTGA ACTACCATCT 
GTTAATACGA TGGAGGAAGA GTATCCTCGG ATAGAGAGTG AATCTACAGT ACATATTGGA 
GTTGGGGAAC CTGACTTCAT CTTGGGACAG CTGTATCATC TGTCTTCAGT ACAGGCACCT 
GAAGTAGCCA AATCTTGGGC CGCATTGGCT AGTTGGGCTT ACAGGTGGGG CAGAAAGGTG 
GTTGACAATG CCAGTCAGGG AGAAGGTGTT CGTCTGTTGC CTAGAGAAAA ATCTGAAGTT 
CAGAATCTGC TCCCAGACAC CATAACCGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT 
GGTCAGGCTG TGTGTCGCCC AGCGGGGATT CAGGATGAAG ATATAACGCT TCAGATCACA 
GAAAGTGAAG ATAATGAGGA AGATGACATG GTTGATGTTA TCTGGCGTCA GTTAATATCA 
AGTTGCCCAT GGCTTTCAGA ACTTGATGAA AGTGCAACAG AAGGAGTCAT TAAAGTGTGG 
AGGAAAGTTG TGGACAGAAT CTTCAGCCTG TATAAACTGT CCTGCAGCGC ATATTTTACT 
TTCCTTAAAC TCAATGCTGG GCAGATTCCT TTAGATGAAG ATGACCCTCG GCTCCATTTA 
AGCCACAGAG TGGAGCAGAG CACGGATGAC ATGATTGTGA TGGCCACGCT GCGGTTGCTC 
AGGTTGCTCG TGAAGCACGC TGGGGAGCTT CGGCAGTATC TGGAACATGG CTTGGAAACA 
ACACCTACTG CGCCTTGGAG AGGAATTATT CCGCAGCTTT TCTCACGCTT AAACCACCCT 
GAAGTGTATG TGCGCCAGAG TATTTGTAAC CTCCTCTGCC GCGTGGCTCA GGATTCCCCG 
CATCTCATAC TGTATCCTGC AATAGTGGGC ACCATATCGC TTAGTAGTGA GTCACAGGCT 
TCAGGAAATA AATTTTCCTC TGCAATTCCA ACTTTACTTG GCAATATTCA AGGAGAAGAA 
CTACTGGTTT CTGAATGTGA GGGAGGAAGT CCTCCTGCTT CTCAAGATAG CAATAAGGAT 
GAACCTAAAA GTGGACTGAA TGAAGACCAA GCCATGATGC AGGACTGCTA CAGCAAAATT 
GTAGATAAAC TGTCCTCGGC AAACCCAACT ATGGTGTTAC AGGTTCAGAT GCTTGTGGCT 
GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAG 
CACATGTATG TCCTGAGACG AATTCAGCAG TTGGAAGACG AGGTGAAGAG AGTCCAGAAC 
AACAACACCT TACGCAAAGA AGAGAAAATC GCAATCATGC GGGAGAAGCA CACAGCTTTG 
ATGAAGCCCA TCGTATTTGC TTTGGAGCAC GTTAGGAGCA TCACAGCGGC CCCTGCAGAA 
ACACCTCATG AAAAGTGGTT TCAGGATAAC TATGGTGATG CCATTGAAAA TGCTCTAGAA 
AAGCTGAAAA CACCGTCAAA CCCTGCAAAG CCTGGAAGCA GCTGGATTCC ATTTAAAGAG 
ATAATGCTGA GTTTGCAACA GAGAGCACAG AAACGTGCAA GTTACATCTT GCGTCTTGAA 
GAAATCAGCC CATGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC CGGAGAAGTC 
TCAGCCAGAG ATACCGTCAC AATCCACAGT GTTGGTGGAA CCATCACAAT CTTACCTACT 
AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGATCAGATG GGAAGAGCTA TCCTTATCTT 
TTCAAAGGAT TGGAGGATTT ACATCTGGAT GAGAGAATAA TGCAATTTCT GTCTATTGTA 
AATACCATGT TTGCAACAAT CAATCGCCAG GAAACACCTC GTTTCCATGC TCGCCACTAT 
TCTGTAACAC CACTGGGAAC TCGCTCAGGC CTAATCCAGT GGGTGGATGG AGCCACGCCC 
TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCCG CCTTGCAAGC ACAAAAGGCC 
CAAGATTCCT ACCAAACTCC TCAGAATCCT GGAATCGTAC CCCGTCCTAG TGAACTTTAT 
TATAGTAAAA TTGGTCCTGC TTTGAAAGCA GTTGGGCTTA GTCTGGATGT GTCCCGCCGG 
GATTGGCCTC TTCATGTGAT GAAGGCAGTG TTAGAAGAGT TAATGGAGGC CACGCCCCCA 
AATCTCCTTG CTAAAGAGCT TTGGTCATCA TGTACCACCC CTGATGAGTG GTGGAGAGTC 
ACACAGTCTT ATGCCAGATC TACTGCAGTC ATGTCTATGG TCGGATACAT AATTGGCCTT 
GGAGATAGAC ATCTGGATAA TGTTCTCATA GATATGACGA CTGGAGAGGT TGTTCACATA 
GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTACCTTTT 
CGAATGACAC AGAACATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGG 
CTTTCATGTG AGCAGGTTCT ACACATTATG CGGCGTGGCA GAGAGACTCT GCTGACGCTG 
CTGGAGGCCT TTGTATATGA CCCCCTGGTG GACTGGACAG CTGGGGGCGA GGCTGGGTTT 
GCTGGTGCGG TCTATGGTGG AGGGGGCCAG CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG 
ATGGAACGAG AGATTACTCG CAGCCTTTTT TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC 
TGGTTTAAAA ACAGAGATGA GATGCTGGTT GTACTTCCCA AGCTGGACAG CAGCTTAGAT 
GAATACCTGA GCTTGCAAGA GCAACTAACA GATGTGGAGA AACTGCAGGG CAAACTGCTG 
GAAGAAATTG AGTTTCTAGA AGGAGCTGAA GGAGTGGATC ATCCTTCTCA TACTCTGCAA 
CACAGGTATT CTGAACACAC TCAACTACAG ACTCAGCAGA GAGCTGTTCA GGAAGCAATC 
CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACACT ATCAGGCTGC GTTCAATAAT 
TTGGAAGCTA CACAGCTGGC CAGTTTGCTT CAGGAGATAA GCACACAGAT GGACCTTGGT 
CCTCCCAGCT ACGTGCCGGC AACAGCCTTT CTGCAGAATG CTGGCCAGGC CCACTTGATT 
AGCCAGTGTG AGCAGCTAGA AGGGGAGGTT GGTGCCCTCC TGCAGCAGAG GCGCTCGGTG 
CTGCGAGGCT GTCTGGAGCA GCTGCATCAC TACGCAACTG TTGCTCTGCA GTATCCAAAG 
GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA GCTCATCTGT 
AATACCACAG TAGAACGCTG TCAGGAACTT TACAGGAAAT ATGAAATGCA GTATGCGCCC 
CAGCCACCCC CAACAGTGTG TCAGTTCATC ACGGCCACTG AGATGACCCT GCAGCGGTAC 
GCAGCCGACA TCAACAGCCG ACTTATCAGA CAGGTGGAAC GCTTGAAACA GGAAGCTGTC 
ACTGTACCGG TTTGTGAAGA TCAGTTGAAA GAAATTGAAC GTTGTATCAA AGTTTTCCTT 
CACGAGAATG GAGAGGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATTTC TGCCCTTTGT 
ACTCTCACAA GGCGTAACCT AATGATGGAA GGTGCAGCGT CGAGTGCTGG AGAGCAGCTG 
GTTGATCTGA CTTCTCGGGA TGGAGCCTGG TTCTTGGAGG AACTCTGCAG TATGAGCGGA 
AATGTCACAT GTCTGGTTCA GTTACTGAAG CAGTGTCATT TAGTGCCACA AGACTTAGAC 
ATTCCTAACC CCATGGAAGC ATCTGAGGCC GTTCATTTGG CTAATGGTGT ATACACCTCA 
CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA 
ATGAAAGGAG AATACACGTT AGAAAATATG CTTCATGAGC TGGACAGTCT TATTGAGCAG 
ACAACTGATG GTGTTCCTCT GCAGACTCTA GTTGAATCTC TTCAAGCCTA TTTAAGAAAT 
GCAGCTATGG GACTTGAGGA AGAAACACAT GCTCATTACA TCGATGTTGC CAGAATGCTT 
CATGCTCAGT ATGGTGAATT AATCCAACCA AGAAATGGTT CAGTTGATGA AACACCAAAA 
ATGTCAGCTG GCCAGATGCT TTTGGTAGCA TTTGATGGCA TGTTTGCCCA AGTTGAGACT 
GCTTTTGGCT TATTGGTTGA AAAGCTAAAT AAGATGGAAA TTCCTATAGC TTGGCGAAAG 
ATTGACATAA TACGGGAAGC CAGGAGCACT CAGGTCAATT TTTTTGATGA TGATAATCAC 
CGACAGGTGC TGGAAGAGAT CTTCTTTCTA AAACGACTAC AGACAATTAA GGAGTTCTTT 
CGGCTCTGTG GTACCTTTTC TAAAACTTTG TCAGGATCAA GTTCACTTGA AGATCAGAAT 
ACTGTAAATG GGCCTGTACA GATTGTCAAT GTGAAAACCC TTTTTAGAAA CTCTTGTTTC 
AGTGAAGACC AGATGGCCAA ACCAATAAAG GCATTCACAG CTGACTTTGT GCGGCAGCTC 
CTGATTGGAC TCCCGAACCA AGCCCTGGGC CTCACACTGT GCAGCTTCAT CAGTGCTCTA 
GGGGTAGACA TCATTGCGCA GGTGGAGGCG AAGGATTTTG GTGCTGAAAG CAAAGTTTCT 
GTTGACGATC TGTGTAAGAA GGCGGTGGAG CACAACATCC AGATCGGGAA GTTCTCTCAG 
CTGGTCATGA ACAGGGCGAC TGTGCTGGCG AGCTCCTACG ACACTGCCTG GAAGAAGCAC 
GACTTGGTGC GCAGGCTGGA GACCAGCATC GCCTCCTGCA AGACAAGCCT GCAGCGTGTC 
CAGCTGCACA TCGCCATGTT TCAGTGGCAG CATGAAGATC TCCTTATCAA TAGACCACAA 
GCCATGTCTG TCACACCTCC CCCTCGTTCT GCCATATTAA CCAGCATGAA AAAGAAACTC 
CATACTCTGA GCCAGATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT GGCAGCACTT 
GAAGCAAGTA TTGAACAGCG ACTCAAATGG GCAGGTGGTG CCAACCCTGC ACTGGCACCT 
GTACTACAGG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAGGAGAGC 
CAAAGAGCAA GTCAGGTCAC TTTCCTCTGC AGCAATATCA TTCATTTTGA AAGTTTACGA 
ACTAGAACTG CAGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAACTAAT CAAACGGTGT 
CAGCAAATGT GTTCATTTGC ATCGCAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT 
TTATTACAGA GGGTGGACAC GACTCTTGAA CATCCTATTG GCAGCTCTGA GTGGCTTTTG 
TCAGCACACA AACAACTGAC CCAGGATATG TCTACTCAGA GAGCAATTCA GACAGAGAAA 
GAGCAACAAA TAGAAACAGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT 
GTGCTCACTG GCCATAACCG GCAGCTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCC 
AAGGATGAAG AAGCTGCTCT GGCAGATGGT GAAGATGTTC CCTATGAGAA CAGTGTTAGG 
CAGTTCTTGG GTGAATATAA ATCATGGCAA GACAACATTC AGACAGTGCT GTTTACATTA 
GTCCAAGCCA TGGGTCAGGT TCGAAGTCAA GAACATGTTG AAATGCTCCA GGAAATAACT 
CCCACCTTGA AAGAACTGAA AACGCAAAGT CAGAGTGTGT ATAATAATTT AGTGAGTTTT 
GCATCACCAT TAGTGACCGA TGCAACAAAT GAATGTTCGA GTCCAACATC GTCTGCTACT 
TATCAACCAT CCTTTGCTGC AGCAGTCCGA AGCAACACTG GCCAGAAGAC TCAGCCTGAT 
GTCATGTCAC AGAACGCTAG AAAGCTGATT CAGAAAAACC TAGCTACATC AGCAGATACC 
CCACCGAGCA CCGTTCCGGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCCATC 
AGAGACCCTA AGACTGGAAA AGCTGTGCAG GAGAGAAACT CGTATGCGGT GAGTGTGTGG 
AAGAGAGTGA AAGCCAAGCT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT 
GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG 
TATGAAGGTT GGACAGCCTG GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840687.1
CDS	335..11041
Translation	MSRRAPGSRLSSGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRIHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSIQHGSRALAIKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRIGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNPSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSVVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANTGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFIECTEQLELLPGENISLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSITENVVKYLKQTSRIAVGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLATEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQSQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEHAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLENMLHELDSLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSIASCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_024984919.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984919.1

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATAATTTA 
AAATACTCTT CATCCAGAGA TAGGGGTGGG TCTTCCTCTT ATGGACTGCA GCCTTCAAAT 
TCAGCTGTGG TGTCTCGGCA AAGGCATGAT GATACCAGAA TCCATGCTGA CATACAGAAT 
GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG 
TCGTTGGGGC AAGAGCTGAG GGTTAACAAT GTGACCAGCC CTGAGTTTAC CAGTATTCAG 
CATGGCAGTC GTGCGTTAGC CATCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCT 
TATTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA 
GACCGAAGGC TGGCTACTGT TAAGCAGTTG AAAGAATTTA TTCAGCAACC AGAAAATAAG 
CTGGTGCTAG TTAAGCAGTT GGATAATATC TTGGCTGCTG TACATGATGT GCTTAATGAA 
AGTAGCAAAT TGCTTCAGGA GTTGAGACAG GAGGGAGCTT GTTGTCTTGG CCTTCTTTGT 
GCATCTCTGA GCTATGAGGC TGAGAAGATC TTCAAATGGA TTTTTAGCAA ATTTAGCTCA 
TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC TTATGTGCCA CCTACAAAGC ACTAGAGACT 
GTAGGAGAAA AGAAAGCCTT TTCATCTGTA ATGCAGCTTG TAATGACCAG CCTGCAGTCC 
ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAGT GTGTTAAGTG CATTCTTTTG 
GTAGCTCGAT GTTACCCTCA TATTTTCAGC ACAAATTTTA GGGATACAGT TGATATATTA 
GTTGGATGGC ATATAGATCA TACTCAGAAG CCTTCACTTA CACAACAGGT GTCTGGGTGG 
TTGCAGAGTT TGGAGCCATT CTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT 
CAGTTTTTAG AGGACATGGA GGCATATGCT GAGGACCTCA GCCATGTAGC CTCTGGGGAG 
TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCCTTAC CAAAGCTGGC AGCACTTCTC 
CGGGTATTCA GTACTGTGGT GAGAAGCATT GGGGAACGTT TCAGCCCAAT TCGAGGTCCT 
CCAATTACTG AGGCATATGT AACAGATGTT CTGTACAGAG TAATGAGATG TGTGACGGCT 
GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT 
TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTTAT TACATATGGA 
TTAGATCAAC TGGAGAATTG CCAGACTTGT GGTACAGATT ATATCATCTC AGTCTTGAAT 
TTACTCACAT TGATTGTTGA ACAGATAAAT ACAAAACTGC CATCATCATT TGTAGAAAAA 
CTGTTTATAC CATCATCTAA GCTACTATTT TTGCGTTATC ATAAAGAAAA AGAGATGTGG 
GCCCTGTCTC CAACTGTGTT TGCACTTCTG AGTAAGAATC TGATGATTGT GCATGGCGAC 
CTGGCTGTTC ATTTCCCTGC CATTCAGTAT GCAGTGCTCT ACACATTGTA TTCTCATTGT 
ACCAGGCATG ACCACTTTAT ATCTAGTAGT CTTAGTTCAT CATCCCCGTC CTTGTTTGAT 
GGTGCCGTTA TTAGTACTGT AACTACAGCT ACAAAAAAAC ATTTCTCAAT TATCTTAAAT 
CTCCTGGGAA TATTGCTTAA GAAAGATAAT CTTAACCAGG ACACAAGGAA ACTGTTAATG 
ACCTGGGCTT TGGAAGTGGC TGTTTTAATG AAGAAGTCTG AAACATATGC ACCTTTATTC 
TCTCTTCCAT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT GGTCGAAGAT 
GTGAATATCT GTCTGCAAGC ATGCAGCAGT CTACATGCAC TGTCCTCTTC CTTACCAGAT 
GATCTTTTAC AGAGGTGTGT TGATGTTTGC CGTGTTCAGC TAGTCCATAG TGGAACTCGT 
ATTCGACAAG CATTTGGAAA GCTATTGAAG TCAATCCCTT TAGATGTTGT CTTGAGCAAT 
AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA 
CCAAGTAACA CATTCCACCC ACAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGA 
AACTCTCATA GAATAGGGAA GGACAATTGG TTAGAAAGAT TGTTCTATAG CTGCCAGAGA 
CTGGATAAAC GTGACCAATC AACGATTCCC CGCAATCTGC TAAAGACAGA TGCTGTCCTC 
TGGCAGTGGG CTATTTGGGA AGCAGCACAG TTCACTGTCC TTTCTAAGCT GAGGACCCCA 
CTTGGCAGAG CTCAGGATAC CTTCCAAACA ATTGAAGGTA TCATTAGAAG TCTTGCAGCT 
CACACATTAA ACCCCGATCA AGATGTTAGT CAGTGGACAA CTGCAGACAA TGACGAAGGC 
CACAGTAGCA ACCAGCTTAG ACTTGTGCTG CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG 
TTAATGTATA ATGCATATGA AGGATGTGCC AATGCATTAA CATCGCCTCC CAAGGTCATT 
AGGACTTTTT TCTATACCAA TCGCCAAACT TGTCAAGACT GGCTGACTCG GATCCGACTC 
TCCATCATGA GGGTTGGATT GTTGGCAGGC CAGCCTGCTG TGACAGTGAG ACACGGCTTC 
GATTTGCTTA CAGAAATGAA GACAAATCCG TCTCAGGGAA ATGAATTGGA AGTAACTATC 
ATGATGGTGG TAGAAGCATT GTGTGAACTT CATTGTCCTG AAGCTATACA GGGAATTGCT 
GTCTGGTCGT CTTCGGTTGT TGGAAAAAAT CTTCTCTGGA TTAATTCAGT GGCCCAACAG 
GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG GAGTACCAGG AGCACCTGTG TGCCATGACA 
GGTGTCGATT GCTGCATCTC TAGCTTCGAC AAATCCGTTC TCACATTGGC TAATACTGGG 
CGTAATAGTG CCAGCCCCAA GCATTCTTTG AATGGTGAAT CCAGAAAAAC TGTGCTTTCC 
AAACCAACTG ATTCTTCTCC TGAGGTTATA AATTATTTGG GAAATAAAGC ATGTGAGTGC 
TACATTTCAA TAGCTGATTG GGCTGCTGTA CAGGAGTGGC AGAATGCTGT CCATGACTTG 
AAAAAGAGTA CCAGTAGTAC TTCTCTCAAC CTAAAGGCTG ATTTCAACTA CATAAAATCA 
CTAAGCAGTT TTGAATCTGG AGAATTTATT GAATGCACTG AACAATTAGA ATTGTTACCA 
GGAGAAAATA TCAGTCTACT TGCTGGAGGA TCAAAAGAAA AAATAGATAT GAAAAAACTG 
CTTCCTAACA TGTTAAGTCC AGATCCAAGA GAACTTCAGA AATCCATTGA AGTTCAGTTG 
TTAAGAAGTT CTGTTTGTTT GGCAACTGCT TTAAACCACA TAGAACAAGA TCAGAAGTGG 
CAGTCTATTA CCGAAAATGT GGTGAAGTAC CTGAAGCAAA CCTCCCGCAT AGCTGTTGGA 
CCCCTGAGAC TTTCTACTCT AACAGTTTCC CAGTCTCTGC CAGTCCTAAG TACTTTACAG 
CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCCAACA GACTTGCCAC AGAGGACTGT 
CTTATTCCAC TCTTTAGTGA AGCTTTGCGT TCATGTAAAC AGCATGATGT CAGGCCATGG 
ATGCAAGCAT TAAGATACAC GATGTACCAG AGTCAGTTGT TGGAGAAAAT AAAAGAACAA 
ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA 
AAACGGGGGA ATGTGTCACT TGCAACAAGA TTGCTGGCAC AGTGTAGCGA GGTTCAGCTG 
GGAAAGACTA CCACTGCACA GGATTTAGTC CAGCACTTTA AAAAACTATC AACTCAAGGT 
CAAGTGGATG AAAAATGGGG GCCTGAACTT GACATTGAAA AAACGAAACT GCTATATACA 
GCAGGCCAGT CAACACATGC AATGGAGATG TTGAGTTCCT GTGCCATCTC TTTCTGCAAG 
TCTGCCAAAG CTGAGCATGC TGTTGCCAAG TCAATTTTGA CATTGGCTAA ATGGATCCAG 
GCAGAATGGA AAGAAATCTC AGGACAGCTG AAACAGGTTT ACAGAGCTCA GCAGCAGCAG 
AACTTCACGG GTCTTTCTAC TTTGTCTAAA AACATACTCA CTCTAATTGA ACTACCATCT 
GTTAATACGA TGGAGGAAGA GTATCCTCGG ATAGAGAGTG AATCTACAGT ACATATTGGA 
GTTGGGGAAC CTGACTTCAT CTTGGGACAG CTGTATCATC TGTCTTCAGT ACAGGCACCT 
GAAGTAGCCA AATCTTGGGC CGCATTGGCT AGTTGGGCTT ACAGGTGGGG CAGAAAGGTG 
GTTGACAATG CCAGTCAGGG AGAAGGTGTT CGTCTGTTGC CTAGAGAAAA ATCTGAAGTT 
CAGAATCTGC TCCCAGACAC CATAACCGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT 
GGTCAGGCTG TGTGTCGCCC AGCGGGGATT CAGGATGAAG ATATAACGCT TCAGATCACA 
GAAAGTGAAG ATAATGAGGA AGATGACATG GTTGATGTTA TCTGGCGTCA GTTAATATCA 
AGTTGCCCAT GGCTTTCAGA ACTTGATGAA AGTGCAACAG AAGGAGTCAT TAAAGTGTGG 
AGGAAAGTTG TGGACAGAAT CTTCAGCCTG TATAAACTGT CCTGCAGCGC ATATTTTACT 
TTCCTTAAAC TCAATGCTGG GCAGATTCCT TTAGATGAAG ATGACCCTCG GCTCCATTTA 
AGCCACAGAG TGGAGCAGAG CACGGATGAC ATGATTGTGA TGGCCACGCT GCGGTTGCTC 
AGGTTGCTCG TGAAGCACGC TGGGGAGCTT CGGCAGTATC TGGAACATGG CTTGGAAACA 
ACACCTACTG CGCCTTGGAG AGGAATTATT CCGCAGCTTT TCTCACGCTT AAACCACCCT 
GAAGTGTATG TGCGCCAGAG TATTTGTAAC CTCCTCTGCC GCGTGGCTCA GGATTCCCCG 
CATCTCATAC TGTATCCTGC AATAGTGGGC ACCATATCGC TTAGTAGTGA GTCACAGGCT 
TCAGGAAATA AATTTTCCTC TGCAATTCCA ACTTTACTTG GCAATATTCA AGGAGAAGAA 
CTACTGGTTT CTGAATGTGA GGGAGGAAGT CCTCCTGCTT CTCAAGATAG CAATAAGGAT 
GAACCTAAAA GTGGACTGAA TGAAGACCAA GCCATGATGC AGGACTGCTA CAGCAAAATT 
GTAGATAAAC TGTCCTCGGC AAACCCAACT ATGGTGTTAC AGGTTCAGAT GCTTGTGGCT 
GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAG 
CACATGTATG TCCTGAGACG AATTCAGCAG TTGGAAGACG AGGTGAAGAG AGTCCAGAAC 
AACAACACCT TACGCAAAGA AGAGAAAATC GCAATCATGC GGGAGAAGCA CACAGCTTTG 
ATGAAGCCCA TCGTATTTGC TTTGGAGCAC GTTAGGAGCA TCACAGCGGC CCCTGCAGAA 
ACACCTCATG AAAAGTGGTT TCAGGATAAC TATGGTGATG CCATTGAAAA TGCTCTAGAA 
AAGCTGAAAA CACCGTCAAA CCCTGCAAAG CCTGGAAGCA GCTGGATTCC ATTTAAAGAG 
ATAATGCTGA GTTTGCAACA GAGAGCACAG AAACGTGCAA GTTACATCTT GCGTCTTGAA 
GAAATCAGCC CATGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC CGGAGAAGTC 
TCAGCCAGAG ATACCGTCAC AATCCACAGT GTTGGTGGAA CCATCACAAT CTTACCTACT 
AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGATCAGATG GGAAGAGCTA TCCTTATCTT 
TTCAAAGGAT TGGAGGATTT ACATCTGGAT GAGAGAATAA TGCAATTTCT GTCTATTGTA 
AATACCATGT TTGCAACAAT CAATCGCCAG GAAACACCTC GTTTCCATGC TCGCCACTAT 
TCTGTAACAC CACTGGGAAC TCGCTCAGGC CTAATCCAGT GGGTGGATGG AGCCACGCCC 
TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCCG CCTTGCAAGC ACAAAAGGCC 
CAAGATTCCT ACCAAACTCC TCAGAATCCT GGAATCGTAC CCCGTCCTAG TGAACTTTAT 
TATAGTAAAA TTGGTCCTGC TTTGAAAGCA GTTGGGCTTA GTCTGGATGT GTCCCGCCGG 
GATTGGCCTC TTCATGTGAT GAAGGCAGTG TTAGAAGAGT TAATGGAGGC CACGCCCCCA 
AATCTCCTTG CTAAAGAGCT TTGGTCATCA TGTACCACCC CTGATGAGTG GTGGAGAGTC 
ACACAGTCTT ATGCCAGATC TACTGCAGTC ATGTCTATGG TCGGATACAT AATTGGCCTT 
GGAGATAGAC ATCTGGATAA TGTTCTCATA GATATGACGA CTGGAGAGGT TGTTCACATA 
GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTACCTTTT 
CGAATGACAC AGAACATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGG 
CTTTCATGTG AGCAGGTTCT ACACATTATG CGGCGTGGCA GAGAGACTCT GCTGACGCTG 
CTGGAGGCCT TTGTATATGA CCCCCTGGTG GACTGGACAG CTGGGGGCGA GGCTGGGTTT 
GCTGGTGCGG TCTATGGTGG AGGGGGCCAG CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG 
ATGGAACGAG AGATTACTCG CAGCCTTTTT TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC 
TGGTTTAAAA ACAGAGATGA GATGCTGGTT GTACTTCCCA AGCTGGACAG CAGCTTAGAT 
GAATACCTGA GCTTGCAAGA GCAACTAACA GATGTGGAGA AACTGCAGGG CAAACTGCTG 
GAAGAAATTG AGTTTCTAGA AGGAGCTGAA GGAGTGGATC ATCCTTCTCA TACTCTGCAA 
CACAGGTATT CTGAACACAC TCAACTACAG ACTCAGCAGA GAGCTGTTCA GGAAGCAATC 
CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACACT ATCAGGCTGC GTTCAATAAT 
TTGGAAGCTA CACAGCTGGC CAGTTTGCTT CAGGAGATAA GCACACAGAT GGACCTTGGT 
CCTCCCAGCT ACGTGCCGGC AACAGCCTTT CTGCAGAATG CTGGCCAGGC CCACTTGATT 
AGCCAGTGTG AGCAGCTAGA AGGGGAGGTT GGTGCCCTCC TGCAGCAGAG GCGCTCGGTG 
CTGCGAGGCT GTCTGGAGCA GCTGCATCAC TACGCAACTG TTGCTCTGCA GTATCCAAAG 
GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA GCTCATCTGT 
AATACCACAG TAGAACGCTG TCAGGAACTT TACAGGAAAT ATGAAATGCA GTATGCGCCC 
CAGCCACCCC CAACAGTGTG TCAGTTCATC ACGGCCACTG AGATGACCCT GCAGCGGTAC 
GCAGCCGACA TCAACAGCCG ACTTATCAGA CAGGTGGAAC GCTTGAAACA GGAAGCTGTC 
ACTGTACCGG TTTGTGAAGA TCAGTTGAAA GAAATTGAAC GTTGTATCAA AGTTTTCCTT 
CACGAGAATG GAGAGGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATTTC TGCCCTTTGT 
ACTCTCACAA GGCGTAACCT AATGATGGAA GGTGCAGCGT CGAGTGCTGG AGAGCAGCTG 
GTTGATCTGA CTTCTCGGGA TGGAGCCTGG TTCTTGGAGG AACTCTGCAG TATGAGCGGA 
AATGTCACAT GTCTGGTTCA GTTACTGAAG CAGTGTCATT TAGTGCCACA AGACTTAGAC 
ATTCCTAACC CCATGGAAGC ATCTGAGGCC GTTCATTTGG CTAATGGTGT ATACACCTCA 
CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA 
ATGAAAGGAG AATACACGTT AGAAAATATG CTTCATGAGC TGGACAGTCT TATTGAGCAG 
ACAACTGATG GTGTTCCTCT GCAGACTCTA GTTGAATCTC TTCAAGCCTA TTTAAGAAAT 
GCAGCTATGG GACTTGAGGA AGAAACACAT GCTCATTACA TCGATGTTGC CAGAATGCTT 
CATGCTCAGT ATGGTGAATT AATCCAACCA AGAAATGGTT CAGTTGATGA AACACCAAAA 
ATGTCAGCTG GCCAGATGCT TTTGGTAGCA TTTGATGGCA TGTTTGCCCA AGTTGAGACT 
GCTTTTGGCT TATTGGTTGA AAAGCTAAAT AAGATGGAAA TTCCTATAGC TTGGCGAAAG 
ATTGACATAA TACGGGAAGC CAGGAGCACT CAGGTCAATT TTTTTGATGA TGATAATCAC 
CGACAGGTGC TGGAAGAGAT CTTCTTTCTA AAACGACTAC AGACAATTAA GGAGTTCTTT 
CGGCTCTGTG GTACCTTTTC TAAAACTTTG TCAGGATCAA GTTCACTTGA AGATCAGAAT 
ACTGTAAATG GGCCTGTACA GATTGTCAAT GTGAAAACCC TTTTTAGAAA CTCTTGTTTC 
AGTGAAGACC AGATGGCCAA ACCAATAAAG GCATTCACAG CTGACTTTGT GCGGCAGCTC 
CTGATTGGAC TCCCGAACCA AGCCCTGGGC CTCACACTGT GCAGCTTCAT CAGTGCTCTA 
GGGGTAGACA TCATTGCGCA GGTGGAGGCG AAGGATTTTG GTGCTGAAAG CAAAGTTTCT 
GTTGACGATC TGTGTAAGAA GGCGGTGGAG CACAACATCC AGATCGGGAA GTTCTCTCAG 
CTGGTCATGA ACAGGGCGAC TGTGCTGGCG AGCTCCTACG ACACTGCCTG GAAGAAGCAC 
GACTTGGTGC GCAGGCTGGA GACCAGCATC GCCTCCTGCA AGACAAGCCT GCAGCGTGTC 
CAGCTGCACA TCGCCATGTT TCAGTGGCAG CATGAAGATC TCCTTATCAA TAGACCACAA 
GCCATGTCTG TCACACCTCC CCCTCGTTCT GCCATATTAA CCAGCATGAA AAAGAAACTC 
CATACTCTGA GCCAGATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT GGCAGCACTT 
GAAGCAAGTA TTGAACAGCG ACTCAAATGG GCAGGTGGTG CCAACCCTGC ACTGGCACCT 
GTACTACAGG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAGGAGAGC 
CAAAGAGCAA GTCAGGTCAC TTTCCTCTGC AGCAATATCA TTCATTTTGA AAGTTTACGA 
ACTAGAACTG CAGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAACTAAT CAAACGGTGT 
CAGCAAATGT GTTCATTTGC ATCGCAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT 
TTATTACAGA GGGTGGACAC GACTCTTGAA CATCCTATTG GCAGCTCTGA GTGGCTTTTG 
TCAGCACACA AACAACTGAC CCAGGATATG TCTACTCAGA GAGCAATTCA GACAGAGAAA 
GAGCAACAAA TAGAAACAGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT 
GTGCTCACTG GCCATAACCG GCAGCTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCC 
AAGGATGAAG AAGCTGCTCT GGCAGATGGT GAAGATGTTC CCTATGAGAA CAGTGTTAGG 
CAGTTCTTGG GTGAATATAA ATCATGGCAA GACAACATTC AGACAGTGCT GTTTACATTA 
GTCCAAGCCA TGGGTCAGGT TCGAAGTCAA GAACATGTTG AAATGCTCCA GGAAATAACT 
CCCACCTTGA AAGAACTGAA AACGCAAAGT CAGAGTGTGT ATAATAATTT AGTGAGTTTT 
GCATCACCAT TAGTGACCGA TGCAACAAAT GAATGTTCGA GTCCAACATC GTCTGCTACT 
TATCAACCAT CCTTTGCTGC AGCAGTCCGA AGCAACACTG GCCAGAAGAC TCAGCCTGAT 
GTCATGTCAC AGAACGCTAG AAAGCTGATT CAGAAAAACC TAGCTACATC AGCAGATACC 
CCACCGAGCA CCGTTCCGGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCCATC 
AGAGACCCTA AGACTGGAAA AGCTGTGCAG GAGAGAAACT CGTATGCGGT GAGTGTGTGG 
AAGAGAGTGA AAGCCAAGCT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT 
GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG 
TATGAAGGTT GGACAGCCTG GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005224744.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224801.1 Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

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CloneID	OBa32978
Clone ID Related Accession (Same CDS sequence)	XM_005224744.4 , XM_005224744.3
Accession Version	XM_005224744.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224744.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
GCATCAGCCG ACCCAGATAA TTTAAAATAC TCTTCATCCA GAGATAGGGG TGGGTCTTCC 
TCTTATGGAC TGCAGCCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA TGATGATACC 
AGAATCCATG CTGACATACA GAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT 
GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC 
AGCCCTGAGT TTACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG 
AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG 
AGGATCACCC GGGAAGATGA CAGAGACCGA AGGCTGGCTA CTGTTAAGCA GTTGAAAGAA 
TTTATTCAGC AACCAGAAAA TAAGCTGGTG CTAGTTAAGC AGTTGGATAA TATCTTGGCT 
GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA 
GCTTGTTGTC TTGGCCTTCT TTGTGCATCT CTGAGCTATG AGGCTGAGAA GATCTTCAAA 
TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TTAAACTCCT CTACTTATGT 
GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG 
CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC 
AAGTGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACAAAT 
TTTAGGGATA CAGTTGATAT ATTAGTTGGA TGGCATATAG ATCATACTCA GAAGCCTTCA 
CTTACACAAC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGACCTT 
GCATTTTCTA CCACTCTTCT TGGTCAGTTT TTAGAGGACA TGGAGGCATA TGCTGAGGAC 
CTCAGCCATG TAGCCTCTGG GGAGTCAGTG GATGAAGATG TTCCTCCTCC ATCAGTGTCC 
TTACCAAAGC TGGCAGCACT TCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA 
CGTTTCAGCC CAATTCGAGG TCCTCCAATT ACTGAGGCAT ATGTAACAGA TGTTCTGTAC 
AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAAGTGTTTT TTTCTGAGGC TGTGTTGACA 
GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT 
TGTGACATGG TTATTACATA TGGATTAGAT CAACTGGAGA ATTGCCAGAC TTGTGGTACA 
GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA 
CTGCCATCAT CATTTGTAGA AAAACTGTTT ATACCATCAT CTAAGCTACT ATTTTTGCGT 
TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGT 
CTGAAGAATA TTCCTGTTTT AGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT 
GCCCTAAACA ATCTGCTACA TAGTCTACAA CTTCCTGATG CCTGTTCTGA AATAAAACAT 
GAGGCTTTTA AGAATCATGT TTTCAATGTA GACAATGCAA AATTTGTAGT TATATTTGAC 
CTTAGTGCTC TGACTACAAT TGGAAATGCT AAAAACTCAC TAATTGGGAT GTGGGCCCTG 
TCTCCAACTG TGTTTGCACT TCTGAGTAAG AATCTGATGA TTGTGCATGG CGACCTGGCT 
GTTCATTTCC CTGCCATTCA GTATGCAGTG CTCTACACAT TGTATTCTCA TTGTACCAGG 
CATGACCACT TTATATCTAG TAGTCTTAGT TCATCATCCC CGTCCTTGTT TGATGGTGCC 
GTTATTAGTA CTGTAACTAC AGCTACAAAA AAACATTTCT CAATTATCTT AAATCTCCTG 
GGAATATTGC TTAAGAAAGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG 
GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ATGCACCTTT ATTCTCTCTT 
CCATCTTTCC ATAAATTTTG CAAAGGCCTT TTAGCCAACA CTCTGGTCGA AGATGTGAAT 
ATCTGTCTGC AAGCATGCAG CAGTCTACAT GCACTGTCCT CTTCCTTACC AGATGATCTT 
TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAGCTAGTCC ATAGTGGAAC TCGTATTCGA 
CAAGCATTTG GAAAGCTATT GAAGTCAATC CCTTTAGATG TTGTCTTGAG CAATAACAAT 
CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT 
AACACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGAAACTCT 
CATAGAATAG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ATAGCTGCCA GAGACTGGAT 
AAACGTGACC AATCAACGAT TCCCCGCAAT CTGCTAAAGA CAGATGCTGT CCTCTGGCAG 
TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGCTGAGGAC CCCACTTGGC 
AGAGCTCAGG ATACCTTCCA AACAATTGAA GGTATCATTA GAAGTCTTGC AGCTCACACA 
TTAAACCCCG ATCAAGATGT TAGTCAGTGG ACAACTGCAG ACAATGACGA AGGCCACAGT 
AGCAACCAGC TTAGACTTGT GCTGCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG 
TATAATGCAT ATGAAGGATG TGCCAATGCA TTAACATCGC CTCCCAAGGT CATTAGGACT 
TTTTTCTATA CCAATCGCCA AACTTGTCAA GACTGGCTGA CTCGGATCCG ACTCTCCATC 
ATGAGGGTTG GATTGTTGGC AGGCCAGCCT GCTGTGACAG TGAGACACGG CTTCGATTTG 
CTTACAGAAA TGAAGACAAA TCCGTCTCAG GGAAATGAAT TGGAAGTAAC TATCATGATG 
GTGGTAGAAG CATTGTGTGA ACTTCATTGT CCTGAAGCTA TACAGGGAAT TGCTGTCTGG 
TCGTCTTCGG TTGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA 
GGGAGGTTTG AAAAGGCCTC TGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTC 
GATTGCTGCA TCTCTAGCTT CGACAAATCC GTTCTCACAT TGGCTAATAC TGGGCGTAAT 
AGTGCCAGCC CCAAGCATTC TTTGAATGGT GAATCCAGAA AAACTGTGCT TTCCAAACCA 
ACTGATTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT 
TCAATAGCTG ATTGGGCTGC TGTACAGGAG TGGCAGAATG CTGTCCATGA CTTGAAAAAG 
AGTACCAGTA GTACTTCTCT CAACCTAAAG GCTGATTTCA ACTACATAAA ATCACTAAGC 
AGTTTTGAAT CTGGAGAATT TATTGAATGC ACTGAACAAT TAGAATTGTT ACCAGGAGAA 
AATATCAGTC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTGCTTCCT 
AACATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TTGAAGTTCA GTTGTTAAGA 
AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CACATAGAAC AAGATCAGAA GTGGCAGTCT 
ATTACCGAAA ATGTGGTGAA GTACCTGAAG CAAACCTCCC GCATAGCTGT TGGACCCCTG 
AGACTTTCTA CTCTAACAGT TTCCCAGTCT CTGCCAGTCC TAAGTACTTT ACAGCTGTAT 
TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTG CCACAGAGGA CTGTCTTATT 
CCACTCTTTA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAA 
GCATTAAGAT ACACGATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT 
CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG 
GGGAATGTGT CACTTGCAAC AAGATTGCTG GCACAGTGTA GCGAGGTTCA GCTGGGAAAG 
ACTACCACTG CACAGGATTT AGTCCAGCAC TTTAAAAAAC TATCAACTCA AGGTCAAGTG 
GATGAAAAAT GGGGGCCTGA ACTTGACATT GAAAAAACGA AACTGCTATA TACAGCAGGC 
CAGTCAACAC ATGCAATGGA GATGTTGAGT TCCTGTGCCA TCTCTTTCTG CAAGTCTGCC 
AAAGCTGAGC ATGCTGTTGC CAAGTCAATT TTGACATTGG CTAAATGGAT CCAGGCAGAA 
TGGAAAGAAA TCTCAGGACA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAGAACTTC 
ACGGGTCTTT CTACTTTGTC TAAAAACATA CTCACTCTAA TTGAACTACC ATCTGTTAAT 
ACGATGGAGG AAGAGTATCC TCGGATAGAG AGTGAATCTA CAGTACATAT TGGAGTTGGG 
GAACCTGACT TCATCTTGGG ACAGCTGTAT CATCTGTCTT CAGTACAGGC ACCTGAAGTA 
GCCAAATCTT GGGCCGCATT GGCTAGTTGG GCTTACAGGT GGGGCAGAAA GGTGGTTGAC 
AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCTAGAG AAAAATCTGA AGTTCAGAAT 
CTGCTCCCAG ACACCATAAC CGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGTCAG 
GCTGTGTGTC GCCCAGCGGG GATTCAGGAT GAAGATATAA CGCTTCAGAT CACAGAAAGT 
GAAGATAATG AGGAAGATGA CATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCAAGTTGC 
CCATGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGAG TCATTAAAGT GTGGAGGAAA 
GTTGTGGACA GAATCTTCAG CCTGTATAAA CTGTCCTGCA GCGCATATTT TACTTTCCTT 
AAACTCAATG CTGGGCAGAT TCCTTTAGAT GAAGATGACC CTCGGCTCCA TTTAAGCCAC 
AGAGTGGAGC AGAGCACGGA TGACATGATT GTGATGGCCA CGCTGCGGTT GCTCAGGTTG 
CTCGTGAAGC ACGCTGGGGA GCTTCGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCT 
ACTGCGCCTT GGAGAGGAAT TATTCCGCAG CTTTTCTCAC GCTTAAACCA CCCTGAAGTG 
TATGTGCGCC AGAGTATTTG TAACCTCCTC TGCCGCGTGG CTCAGGATTC CCCGCATCTC 
ATACTGTATC CTGCAATAGT GGGCACCATA TCGCTTAGTA GTGAGTCACA GGCTTCAGGA 
AATAAATTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAACTACTG 
GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCTCAAG ATAGCAATAA GGATGAACCT 
AAAAGTGGAC TGAATGAAGA CCAAGCCATG ATGCAGGACT GCTACAGCAA AATTGTAGAT 
AAACTGTCCT CGGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTTGT GGCTGAGCTG 
CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG 
TATGTCCTGA GACGAATTCA GCAGTTGGAA GACGAGGTGA AGAGAGTCCA GAACAACAAC 
ACCTTACGCA AAGAAGAGAA AATCGCAATC ATGCGGGAGA AGCACACAGC TTTGATGAAG 
CCCATCGTAT TTGCTTTGGA GCACGTTAGG AGCATCACAG CGGCCCCTGC AGAAACACCT 
CATGAAAAGT GGTTTCAGGA TAACTATGGT GATGCCATTG AAAATGCTCT AGAAAAGCTG 
AAAACACCGT CAAACCCTGC AAAGCCTGGA AGCAGCTGGA TTCCATTTAA AGAGATAATG 
CTGAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGTTACA TCTTGCGTCT TGAAGAAATC 
AGCCCATGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCCGGAGA AGTCTCAGCC 
AGAGATACCG TCACAATCCA CAGTGTTGGT GGAACCATCA CAATCTTACC TACTAAAACC 
AAGCCAAAGA AACTTCTCTT TCTAGGATCA GATGGGAAGA GCTATCCTTA TCTTTTCAAA 
GGATTGGAGG ATTTACATCT GGATGAGAGA ATAATGCAAT TTCTGTCTAT TGTAAATACC 
ATGTTTGCAA CAATCAATCG CCAGGAAACA CCTCGTTTCC ATGCTCGCCA CTATTCTGTA 
ACACCACTGG GAACTCGCTC AGGCCTAATC CAGTGGGTGG ATGGAGCCAC GCCCTTATTT 
GGTCTTTACA AACGATGGCA ACAACGGGAA GCCGCCTTGC AAGCACAAAA GGCCCAAGAT 
TCCTACCAAA CTCCTCAGAA TCCTGGAATC GTACCCCGTC CTAGTGAACT TTATTATAGT 
AAAATTGGTC CTGCTTTGAA AGCAGTTGGG CTTAGTCTGG ATGTGTCCCG CCGGGATTGG 
CCTCTTCATG TGATGAAGGC AGTGTTAGAA GAGTTAATGG AGGCCACGCC CCCAAATCTC 
CTTGCTAAAG AGCTTTGGTC ATCATGTACC ACCCCTGATG AGTGGTGGAG AGTCACACAG 
TCTTATGCCA GATCTACTGC AGTCATGTCT ATGGTCGGAT ACATAATTGG CCTTGGAGAT 
AGACATCTGG ATAATGTTCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC 
AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTACC TTTTCGAATG 
ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGGCTTTCA 
TGTGAGCAGG TTCTACACAT TATGCGGCGT GGCAGAGAGA CTCTGCTGAC GCTGCTGGAG 
GCCTTTGTAT ATGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT 
GCGGTCTATG GTGGAGGGGG CCAGCAGGCT GAGAGCAAGC AGAGCAAGAG AGAGATGGAA 
CGAGAGATTA CTCGCAGCCT TTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT 
AAAAACAGAG ATGAGATGCT GGTTGTACTT CCCAAGCTGG ACAGCAGCTT AGATGAATAC 
CTGAGCTTGC AAGAGCAACT AACAGATGTG GAGAAACTGC AGGGCAAACT GCTGGAAGAA 
ATTGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG 
TATTCTGAAC ACACTCAACT ACAGACTCAG CAGAGAGCTG TTCAGGAAGC AATCCAGGTG 
AAGCTGAATG AATTTGAACA GTGGATAACA CACTATCAGG CTGCGTTCAA TAATTTGGAA 
GCTACACAGC TGGCCAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT TGGTCCTCCC 
AGCTACGTGC CGGCAACAGC CTTTCTGCAG AATGCTGGCC AGGCCCACTT GATTAGCCAG 
TGTGAGCAGC TAGAAGGGGA GGTTGGTGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA 
GGCTGTCTGG AGCAGCTGCA TCACTACGCA ACTGTTGCTC TGCAGTATCC AAAGGCCATA 
TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAGCTCAT CTGTAATACC 
ACAGTAGAAC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC GCCCCAGCCA 
CCCCCAACAG TGTGTCAGTT CATCACGGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCC 
GACATCAACA GCCGACTTAT CAGACAGGTG GAACGCTTGA AACAGGAAGC TGTCACTGTA 
CCGGTTTGTG AAGATCAGTT GAAAGAAATT GAACGTTGTA TCAAAGTTTT CCTTCACGAG 
AATGGAGAGG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TTTCTGCCCT TTGTACTCTC 
ACAAGGCGTA ACCTAATGAT GGAAGGTGCA GCGTCGAGTG CTGGAGAGCA GCTGGTTGAT 
CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAACTCT GCAGTATGAG CGGAAATGTC 
ACATGTCTGG TTCAGTTACT GAAGCAGTGT CATTTAGTGC CACAAGACTT AGACATTCCT 
AACCCCATGG AAGCATCTGA GGCCGTTCAT TTGGCTAATG GTGTATACAC CTCACTTCAG 
GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA 
GGAGAATACA CGTTAGAAAA TATGCTTCAT GAGCTGGACA GTCTTATTGA GCAGACAACT 
GATGGTGTTC CTCTGCAGAC TCTAGTTGAA TCTCTTCAAG CCTATTTAAG AAATGCAGCT 
ATGGGACTTG AGGAAGAAAC ACATGCTCAT TACATCGATG TTGCCAGAAT GCTTCATGCT 
CAGTATGGTG AATTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA 
GCTGGCCAGA TGCTTTTGGT AGCATTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT 
GGCTTATTGG TTGAAAAGCT AAATAAGATG GAAATTCCTA TAGCTTGGCG AAAGATTGAC 
ATAATACGGG AAGCCAGGAG CACTCAGGTC AATTTTTTTG ATGATGATAA TCACCGACAG 
GTGCTGGAAG AGATCTTCTT TCTAAAACGA CTACAGACAA TTAAGGAGTT CTTTCGGCTC 
TGTGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA 
AATGGGCCTG TACAGATTGT CAATGTGAAA ACCCTTTTTA GAAACTCTTG TTTCAGTGAA 
GACCAGATGG CCAAACCAAT AAAGGCATTC ACAGCTGACT TTGTGCGGCA GCTCCTGATT 
GGACTCCCGA ACCAAGCCCT GGGCCTCACA CTGTGCAGCT TCATCAGTGC TCTAGGGGTA 
GACATCATTG CGCAGGTGGA GGCGAAGGAT TTTGGTGCTG AAAGCAAAGT TTCTGTTGAC 
GATCTGTGTA AGAAGGCGGT GGAGCACAAC ATCCAGATCG GGAAGTTCTC TCAGCTGGTC 
ATGAACAGGG CGACTGTGCT GGCGAGCTCC TACGACACTG CCTGGAAGAA GCACGACTTG 
GTGCGCAGGC TGGAGACCAG CATCGCCTCC TGCAAGACAA GCCTGCAGCG TGTCCAGCTG 
CACATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAATAGACC ACAAGCCATG 
TCTGTCACAC CTCCCCCTCG TTCTGCCATA TTAACCAGCA TGAAAAAGAA ACTCCATACT 
CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC ACTTGAAGCA 
AGTATTGAAC AGCGACTCAA ATGGGCAGGT GGTGCCAACC CTGCACTGGC ACCTGTACTA 
CAGGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAGGA GAGCCAAAGA 
GCAAGTCAGG TCACTTTCCT CTGCAGCAAT ATCATTCATT TTGAAAGTTT ACGAACTAGA 
ACTGCAGAAG CCTTAAACCT GGATGCTGCA TTATTTGAAC TAATCAAACG GTGTCAGCAA 
ATGTGTTCAT TTGCATCGCA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA 
CAGAGGGTGG ACACGACTCT TGAACATCCT ATTGGCAGCT CTGAGTGGCT TTTGTCAGCA 
CACAAACAAC TGACCCAGGA TATGTCTACT CAGAGAGCAA TTCAGACAGA GAAAGAGCAA 
CAAATAGAAA CAGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC 
ACTGGCCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT 
GAAGAAGCTG CTCTGGCAGA TGGTGAAGAT GTTCCCTATG AGAACAGTGT TAGGCAGTTC 
TTGGGTGAAT ATAAATCATG GCAAGACAAC ATTCAGACAG TGCTGTTTAC ATTAGTCCAA 
GCCATGGGTC AGGTTCGAAG TCAAGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC 
TTGAAAGAAC TGAAAACGCA AAGTCAGAGT GTGTATAATA ATTTAGTGAG TTTTGCATCA 
CCATTAGTGA CCGATGCAAC AAATGAATGT TCGAGTCCAA CATCGTCTGC TACTTATCAA 
CCATCCTTTG CTGCAGCAGT CCGAAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG 
TCACAGAACG CTAGAAAGCT GATTCAGAAA AACCTAGCTA CATCAGCAGA TACCCCACCG 
AGCACCGTTC CGGGAACTGG CAAGAGTGTT GCTTGTAGTC CTAAAAAGGC CATCAGAGAC 
CCTAAGACTG GAAAAGCTGT GCAGGAGAGA AACTCGTATG CGGTGAGTGT GTGGAAGAGA 
GTGAAAGCCA AGCTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA 
CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA 
GGTTGGACAG CCTGGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224801.1
CDS	147..11087
Translation	MQKHHKGSILYSMRFLRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRIHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSIQHGSRALAIKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRIGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNPSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSVVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANTGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFIECTEQLELLPGENISLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSITENVVKYLKQTSRIAVGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLATEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQSQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEHAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLENMLHELDSLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSIASCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005224744.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224744.4

ORF Insert Sequence:

ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
GCATCAGCCG ACCCAGATAA TTTAAAATAC TCTTCATCCA GAGATAGGGG TGGGTCTTCC 
TCTTATGGAC TGCAGCCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA TGATGATACC 
AGAATCCATG CTGACATACA GAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT 
GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC 
AGCCCTGAGT TTACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG 
AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG 
AGGATCACCC GGGAAGATGA CAGAGACCGA AGGCTGGCTA CTGTTAAGCA GTTGAAAGAA 
TTTATTCAGC AACCAGAAAA TAAGCTGGTG CTAGTTAAGC AGTTGGATAA TATCTTGGCT 
GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA 
GCTTGTTGTC TTGGCCTTCT TTGTGCATCT CTGAGCTATG AGGCTGAGAA GATCTTCAAA 
TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TTAAACTCCT CTACTTATGT 
GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG 
CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC 
AAGTGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACAAAT 
TTTAGGGATA CAGTTGATAT ATTAGTTGGA TGGCATATAG ATCATACTCA GAAGCCTTCA 
CTTACACAAC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGACCTT 
GCATTTTCTA CCACTCTTCT TGGTCAGTTT TTAGAGGACA TGGAGGCATA TGCTGAGGAC 
CTCAGCCATG TAGCCTCTGG GGAGTCAGTG GATGAAGATG TTCCTCCTCC ATCAGTGTCC 
TTACCAAAGC TGGCAGCACT TCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA 
CGTTTCAGCC CAATTCGAGG TCCTCCAATT ACTGAGGCAT ATGTAACAGA TGTTCTGTAC 
AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAAGTGTTTT TTTCTGAGGC TGTGTTGACA 
GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT 
TGTGACATGG TTATTACATA TGGATTAGAT CAACTGGAGA ATTGCCAGAC TTGTGGTACA 
GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA 
CTGCCATCAT CATTTGTAGA AAAACTGTTT ATACCATCAT CTAAGCTACT ATTTTTGCGT 
TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGT 
CTGAAGAATA TTCCTGTTTT AGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT 
GCCCTAAACA ATCTGCTACA TAGTCTACAA CTTCCTGATG CCTGTTCTGA AATAAAACAT 
GAGGCTTTTA AGAATCATGT TTTCAATGTA GACAATGCAA AATTTGTAGT TATATTTGAC 
CTTAGTGCTC TGACTACAAT TGGAAATGCT AAAAACTCAC TAATTGGGAT GTGGGCCCTG 
TCTCCAACTG TGTTTGCACT TCTGAGTAAG AATCTGATGA TTGTGCATGG CGACCTGGCT 
GTTCATTTCC CTGCCATTCA GTATGCAGTG CTCTACACAT TGTATTCTCA TTGTACCAGG 
CATGACCACT TTATATCTAG TAGTCTTAGT TCATCATCCC CGTCCTTGTT TGATGGTGCC 
GTTATTAGTA CTGTAACTAC AGCTACAAAA AAACATTTCT CAATTATCTT AAATCTCCTG 
GGAATATTGC TTAAGAAAGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG 
GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ATGCACCTTT ATTCTCTCTT 
CCATCTTTCC ATAAATTTTG CAAAGGCCTT TTAGCCAACA CTCTGGTCGA AGATGTGAAT 
ATCTGTCTGC AAGCATGCAG CAGTCTACAT GCACTGTCCT CTTCCTTACC AGATGATCTT 
TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAGCTAGTCC ATAGTGGAAC TCGTATTCGA 
CAAGCATTTG GAAAGCTATT GAAGTCAATC CCTTTAGATG TTGTCTTGAG CAATAACAAT 
CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT 
AACACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGAAACTCT 
CATAGAATAG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ATAGCTGCCA GAGACTGGAT 
AAACGTGACC AATCAACGAT TCCCCGCAAT CTGCTAAAGA CAGATGCTGT CCTCTGGCAG 
TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGCTGAGGAC CCCACTTGGC 
AGAGCTCAGG ATACCTTCCA AACAATTGAA GGTATCATTA GAAGTCTTGC AGCTCACACA 
TTAAACCCCG ATCAAGATGT TAGTCAGTGG ACAACTGCAG ACAATGACGA AGGCCACAGT 
AGCAACCAGC TTAGACTTGT GCTGCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG 
TATAATGCAT ATGAAGGATG TGCCAATGCA TTAACATCGC CTCCCAAGGT CATTAGGACT 
TTTTTCTATA CCAATCGCCA AACTTGTCAA GACTGGCTGA CTCGGATCCG ACTCTCCATC 
ATGAGGGTTG GATTGTTGGC AGGCCAGCCT GCTGTGACAG TGAGACACGG CTTCGATTTG 
CTTACAGAAA TGAAGACAAA TCCGTCTCAG GGAAATGAAT TGGAAGTAAC TATCATGATG 
GTGGTAGAAG CATTGTGTGA ACTTCATTGT CCTGAAGCTA TACAGGGAAT TGCTGTCTGG 
TCGTCTTCGG TTGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA 
GGGAGGTTTG AAAAGGCCTC TGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTC 
GATTGCTGCA TCTCTAGCTT CGACAAATCC GTTCTCACAT TGGCTAATAC TGGGCGTAAT 
AGTGCCAGCC CCAAGCATTC TTTGAATGGT GAATCCAGAA AAACTGTGCT TTCCAAACCA 
ACTGATTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT 
TCAATAGCTG ATTGGGCTGC TGTACAGGAG TGGCAGAATG CTGTCCATGA CTTGAAAAAG 
AGTACCAGTA GTACTTCTCT CAACCTAAAG GCTGATTTCA ACTACATAAA ATCACTAAGC 
AGTTTTGAAT CTGGAGAATT TATTGAATGC ACTGAACAAT TAGAATTGTT ACCAGGAGAA 
AATATCAGTC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTGCTTCCT 
AACATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TTGAAGTTCA GTTGTTAAGA 
AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CACATAGAAC AAGATCAGAA GTGGCAGTCT 
ATTACCGAAA ATGTGGTGAA GTACCTGAAG CAAACCTCCC GCATAGCTGT TGGACCCCTG 
AGACTTTCTA CTCTAACAGT TTCCCAGTCT CTGCCAGTCC TAAGTACTTT ACAGCTGTAT 
TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTG CCACAGAGGA CTGTCTTATT 
CCACTCTTTA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAA 
GCATTAAGAT ACACGATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT 
CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG 
GGGAATGTGT CACTTGCAAC AAGATTGCTG GCACAGTGTA GCGAGGTTCA GCTGGGAAAG 
ACTACCACTG CACAGGATTT AGTCCAGCAC TTTAAAAAAC TATCAACTCA AGGTCAAGTG 
GATGAAAAAT GGGGGCCTGA ACTTGACATT GAAAAAACGA AACTGCTATA TACAGCAGGC 
CAGTCAACAC ATGCAATGGA GATGTTGAGT TCCTGTGCCA TCTCTTTCTG CAAGTCTGCC 
AAAGCTGAGC ATGCTGTTGC CAAGTCAATT TTGACATTGG CTAAATGGAT CCAGGCAGAA 
TGGAAAGAAA TCTCAGGACA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAGAACTTC 
ACGGGTCTTT CTACTTTGTC TAAAAACATA CTCACTCTAA TTGAACTACC ATCTGTTAAT 
ACGATGGAGG AAGAGTATCC TCGGATAGAG AGTGAATCTA CAGTACATAT TGGAGTTGGG 
GAACCTGACT TCATCTTGGG ACAGCTGTAT CATCTGTCTT CAGTACAGGC ACCTGAAGTA 
GCCAAATCTT GGGCCGCATT GGCTAGTTGG GCTTACAGGT GGGGCAGAAA GGTGGTTGAC 
AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCTAGAG AAAAATCTGA AGTTCAGAAT 
CTGCTCCCAG ACACCATAAC CGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGTCAG 
GCTGTGTGTC GCCCAGCGGG GATTCAGGAT GAAGATATAA CGCTTCAGAT CACAGAAAGT 
GAAGATAATG AGGAAGATGA CATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCAAGTTGC 
CCATGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGAG TCATTAAAGT GTGGAGGAAA 
GTTGTGGACA GAATCTTCAG CCTGTATAAA CTGTCCTGCA GCGCATATTT TACTTTCCTT 
AAACTCAATG CTGGGCAGAT TCCTTTAGAT GAAGATGACC CTCGGCTCCA TTTAAGCCAC 
AGAGTGGAGC AGAGCACGGA TGACATGATT GTGATGGCCA CGCTGCGGTT GCTCAGGTTG 
CTCGTGAAGC ACGCTGGGGA GCTTCGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCT 
ACTGCGCCTT GGAGAGGAAT TATTCCGCAG CTTTTCTCAC GCTTAAACCA CCCTGAAGTG 
TATGTGCGCC AGAGTATTTG TAACCTCCTC TGCCGCGTGG CTCAGGATTC CCCGCATCTC 
ATACTGTATC CTGCAATAGT GGGCACCATA TCGCTTAGTA GTGAGTCACA GGCTTCAGGA 
AATAAATTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAACTACTG 
GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCTCAAG ATAGCAATAA GGATGAACCT 
AAAAGTGGAC TGAATGAAGA CCAAGCCATG ATGCAGGACT GCTACAGCAA AATTGTAGAT 
AAACTGTCCT CGGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTTGT GGCTGAGCTG 
CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG 
TATGTCCTGA GACGAATTCA GCAGTTGGAA GACGAGGTGA AGAGAGTCCA GAACAACAAC 
ACCTTACGCA AAGAAGAGAA AATCGCAATC ATGCGGGAGA AGCACACAGC TTTGATGAAG 
CCCATCGTAT TTGCTTTGGA GCACGTTAGG AGCATCACAG CGGCCCCTGC AGAAACACCT 
CATGAAAAGT GGTTTCAGGA TAACTATGGT GATGCCATTG AAAATGCTCT AGAAAAGCTG 
AAAACACCGT CAAACCCTGC AAAGCCTGGA AGCAGCTGGA TTCCATTTAA AGAGATAATG 
CTGAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGTTACA TCTTGCGTCT TGAAGAAATC 
AGCCCATGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCCGGAGA AGTCTCAGCC 
AGAGATACCG TCACAATCCA CAGTGTTGGT GGAACCATCA CAATCTTACC TACTAAAACC 
AAGCCAAAGA AACTTCTCTT TCTAGGATCA GATGGGAAGA GCTATCCTTA TCTTTTCAAA 
GGATTGGAGG ATTTACATCT GGATGAGAGA ATAATGCAAT TTCTGTCTAT TGTAAATACC 
ATGTTTGCAA CAATCAATCG CCAGGAAACA CCTCGTTTCC ATGCTCGCCA CTATTCTGTA 
ACACCACTGG GAACTCGCTC AGGCCTAATC CAGTGGGTGG ATGGAGCCAC GCCCTTATTT 
GGTCTTTACA AACGATGGCA ACAACGGGAA GCCGCCTTGC AAGCACAAAA GGCCCAAGAT 
TCCTACCAAA CTCCTCAGAA TCCTGGAATC GTACCCCGTC CTAGTGAACT TTATTATAGT 
AAAATTGGTC CTGCTTTGAA AGCAGTTGGG CTTAGTCTGG ATGTGTCCCG CCGGGATTGG 
CCTCTTCATG TGATGAAGGC AGTGTTAGAA GAGTTAATGG AGGCCACGCC CCCAAATCTC 
CTTGCTAAAG AGCTTTGGTC ATCATGTACC ACCCCTGATG AGTGGTGGAG AGTCACACAG 
TCTTATGCCA GATCTACTGC AGTCATGTCT ATGGTCGGAT ACATAATTGG CCTTGGAGAT 
AGACATCTGG ATAATGTTCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC 
AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTACC TTTTCGAATG 
ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGGCTTTCA 
TGTGAGCAGG TTCTACACAT TATGCGGCGT GGCAGAGAGA CTCTGCTGAC GCTGCTGGAG 
GCCTTTGTAT ATGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT 
GCGGTCTATG GTGGAGGGGG CCAGCAGGCT GAGAGCAAGC AGAGCAAGAG AGAGATGGAA 
CGAGAGATTA CTCGCAGCCT TTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT 
AAAAACAGAG ATGAGATGCT GGTTGTACTT CCCAAGCTGG ACAGCAGCTT AGATGAATAC 
CTGAGCTTGC AAGAGCAACT AACAGATGTG GAGAAACTGC AGGGCAAACT GCTGGAAGAA 
ATTGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG 
TATTCTGAAC ACACTCAACT ACAGACTCAG CAGAGAGCTG TTCAGGAAGC AATCCAGGTG 
AAGCTGAATG AATTTGAACA GTGGATAACA CACTATCAGG CTGCGTTCAA TAATTTGGAA 
GCTACACAGC TGGCCAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT TGGTCCTCCC 
AGCTACGTGC CGGCAACAGC CTTTCTGCAG AATGCTGGCC AGGCCCACTT GATTAGCCAG 
TGTGAGCAGC TAGAAGGGGA GGTTGGTGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA 
GGCTGTCTGG AGCAGCTGCA TCACTACGCA ACTGTTGCTC TGCAGTATCC AAAGGCCATA 
TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAGCTCAT CTGTAATACC 
ACAGTAGAAC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC GCCCCAGCCA 
CCCCCAACAG TGTGTCAGTT CATCACGGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCC 
GACATCAACA GCCGACTTAT CAGACAGGTG GAACGCTTGA AACAGGAAGC TGTCACTGTA 
CCGGTTTGTG AAGATCAGTT GAAAGAAATT GAACGTTGTA TCAAAGTTTT CCTTCACGAG 
AATGGAGAGG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TTTCTGCCCT TTGTACTCTC 
ACAAGGCGTA ACCTAATGAT GGAAGGTGCA GCGTCGAGTG CTGGAGAGCA GCTGGTTGAT 
CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAACTCT GCAGTATGAG CGGAAATGTC 
ACATGTCTGG TTCAGTTACT GAAGCAGTGT CATTTAGTGC CACAAGACTT AGACATTCCT 
AACCCCATGG AAGCATCTGA GGCCGTTCAT TTGGCTAATG GTGTATACAC CTCACTTCAG 
GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA 
GGAGAATACA CGTTAGAAAA TATGCTTCAT GAGCTGGACA GTCTTATTGA GCAGACAACT 
GATGGTGTTC CTCTGCAGAC TCTAGTTGAA TCTCTTCAAG CCTATTTAAG AAATGCAGCT 
ATGGGACTTG AGGAAGAAAC ACATGCTCAT TACATCGATG TTGCCAGAAT GCTTCATGCT 
CAGTATGGTG AATTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA 
GCTGGCCAGA TGCTTTTGGT AGCATTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT 
GGCTTATTGG TTGAAAAGCT AAATAAGATG GAAATTCCTA TAGCTTGGCG AAAGATTGAC 
ATAATACGGG AAGCCAGGAG CACTCAGGTC AATTTTTTTG ATGATGATAA TCACCGACAG 
GTGCTGGAAG AGATCTTCTT TCTAAAACGA CTACAGACAA TTAAGGAGTT CTTTCGGCTC 
TGTGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA 
AATGGGCCTG TACAGATTGT CAATGTGAAA ACCCTTTTTA GAAACTCTTG TTTCAGTGAA 
GACCAGATGG CCAAACCAAT AAAGGCATTC ACAGCTGACT TTGTGCGGCA GCTCCTGATT 
GGACTCCCGA ACCAAGCCCT GGGCCTCACA CTGTGCAGCT TCATCAGTGC TCTAGGGGTA 
GACATCATTG CGCAGGTGGA GGCGAAGGAT TTTGGTGCTG AAAGCAAAGT TTCTGTTGAC 
GATCTGTGTA AGAAGGCGGT GGAGCACAAC ATCCAGATCG GGAAGTTCTC TCAGCTGGTC 
ATGAACAGGG CGACTGTGCT GGCGAGCTCC TACGACACTG CCTGGAAGAA GCACGACTTG 
GTGCGCAGGC TGGAGACCAG CATCGCCTCC TGCAAGACAA GCCTGCAGCG TGTCCAGCTG 
CACATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAATAGACC ACAAGCCATG 
TCTGTCACAC CTCCCCCTCG TTCTGCCATA TTAACCAGCA TGAAAAAGAA ACTCCATACT 
CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC ACTTGAAGCA 
AGTATTGAAC AGCGACTCAA ATGGGCAGGT GGTGCCAACC CTGCACTGGC ACCTGTACTA 
CAGGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAGGA GAGCCAAAGA 
GCAAGTCAGG TCACTTTCCT CTGCAGCAAT ATCATTCATT TTGAAAGTTT ACGAACTAGA 
ACTGCAGAAG CCTTAAACCT GGATGCTGCA TTATTTGAAC TAATCAAACG GTGTCAGCAA 
ATGTGTTCAT TTGCATCGCA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA 
CAGAGGGTGG ACACGACTCT TGAACATCCT ATTGGCAGCT CTGAGTGGCT TTTGTCAGCA 
CACAAACAAC TGACCCAGGA TATGTCTACT CAGAGAGCAA TTCAGACAGA GAAAGAGCAA 
CAAATAGAAA CAGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC 
ACTGGCCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT 
GAAGAAGCTG CTCTGGCAGA TGGTGAAGAT GTTCCCTATG AGAACAGTGT TAGGCAGTTC 
TTGGGTGAAT ATAAATCATG GCAAGACAAC ATTCAGACAG TGCTGTTTAC ATTAGTCCAA 
GCCATGGGTC AGGTTCGAAG TCAAGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC 
TTGAAAGAAC TGAAAACGCA AAGTCAGAGT GTGTATAATA ATTTAGTGAG TTTTGCATCA 
CCATTAGTGA CCGATGCAAC AAATGAATGT TCGAGTCCAA CATCGTCTGC TACTTATCAA 
CCATCCTTTG CTGCAGCAGT CCGAAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG 
TCACAGAACG CTAGAAAGCT GATTCAGAAA AACCTAGCTA CATCAGCAGA TACCCCACCG 
AGCACCGTTC CGGGAACTGG CAAGAGTGTT GCTTGTAGTC CTAAAAAGGC CATCAGAGAC 
CCTAAGACTG GAAAAGCTGT GCAGGAGAGA AACTCGTATG CGGTGAGTGT GTGGAAGAGA 
GTGAAAGCCA AGCTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA 
CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA 
GGTTGGACAG CCTGGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005224744.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224801.1 Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X1, mRNA.

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CloneID	OBa32978
Clone ID Related Accession (Same CDS sequence)	XM_005224744.4 , XM_005224744.3
Accession Version	XM_005224744.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
GCATCAGCCG ACCCAGATAA TTTAAAATAC TCTTCATCCA GAGATAGGGG TGGGTCTTCC 
TCTTATGGAC TGCAGCCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA TGATGATACC 
AGAATCCATG CTGACATACA GAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT 
GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC 
AGCCCTGAGT TTACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG 
AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG 
AGGATCACCC GGGAAGATGA CAGAGACCGA AGGCTGGCTA CTGTTAAGCA GTTGAAAGAA 
TTTATTCAGC AACCAGAAAA TAAGCTGGTG CTAGTTAAGC AGTTGGATAA TATCTTGGCT 
GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA 
GCTTGTTGTC TTGGCCTTCT TTGTGCATCT CTGAGCTATG AGGCTGAGAA GATCTTCAAA 
TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TTAAACTCCT CTACTTATGT 
GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG 
CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC 
AAGTGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACAAAT 
TTTAGGGATA CAGTTGATAT ATTAGTTGGA TGGCATATAG ATCATACTCA GAAGCCTTCA 
CTTACACAAC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGACCTT 
GCATTTTCTA CCACTCTTCT TGGTCAGTTT TTAGAGGACA TGGAGGCATA TGCTGAGGAC 
CTCAGCCATG TAGCCTCTGG GGAGTCAGTG GATGAAGATG TTCCTCCTCC ATCAGTGTCC 
TTACCAAAGC TGGCAGCACT TCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA 
CGTTTCAGCC CAATTCGAGG TCCTCCAATT ACTGAGGCAT ATGTAACAGA TGTTCTGTAC 
AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAAGTGTTTT TTTCTGAGGC TGTGTTGACA 
GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT 
TGTGACATGG TTATTACATA TGGATTAGAT CAACTGGAGA ATTGCCAGAC TTGTGGTACA 
GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA 
CTGCCATCAT CATTTGTAGA AAAACTGTTT ATACCATCAT CTAAGCTACT ATTTTTGCGT 
TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGT 
CTGAAGAATA TTCCTGTTTT AGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT 
GCCCTAAACA ATCTGCTACA TAGTCTACAA CTTCCTGATG CCTGTTCTGA AATAAAACAT 
GAGGCTTTTA AGAATCATGT TTTCAATGTA GACAATGCAA AATTTGTAGT TATATTTGAC 
CTTAGTGCTC TGACTACAAT TGGAAATGCT AAAAACTCAC TAATTGGGAT GTGGGCCCTG 
TCTCCAACTG TGTTTGCACT TCTGAGTAAG AATCTGATGA TTGTGCATGG CGACCTGGCT 
GTTCATTTCC CTGCCATTCA GTATGCAGTG CTCTACACAT TGTATTCTCA TTGTACCAGG 
CATGACCACT TTATATCTAG TAGTCTTAGT TCATCATCCC CGTCCTTGTT TGATGGTGCC 
GTTATTAGTA CTGTAACTAC AGCTACAAAA AAACATTTCT CAATTATCTT AAATCTCCTG 
GGAATATTGC TTAAGAAAGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG 
GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ATGCACCTTT ATTCTCTCTT 
CCATCTTTCC ATAAATTTTG CAAAGGCCTT TTAGCCAACA CTCTGGTCGA AGATGTGAAT 
ATCTGTCTGC AAGCATGCAG CAGTCTACAT GCACTGTCCT CTTCCTTACC AGATGATCTT 
TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAGCTAGTCC ATAGTGGAAC TCGTATTCGA 
CAAGCATTTG GAAAGCTATT GAAGTCAATC CCTTTAGATG TTGTCTTGAG CAATAACAAT 
CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT 
AACACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGAAACTCT 
CATAGAATAG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ATAGCTGCCA GAGACTGGAT 
AAACGTGACC AATCAACGAT TCCCCGCAAT CTGCTAAAGA CAGATGCTGT CCTCTGGCAG 
TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGCTGAGGAC CCCACTTGGC 
AGAGCTCAGG ATACCTTCCA AACAATTGAA GGTATCATTA GAAGTCTTGC AGCTCACACA 
TTAAACCCCG ATCAAGATGT TAGTCAGTGG ACAACTGCAG ACAATGACGA AGGCCACAGT 
AGCAACCAGC TTAGACTTGT GCTGCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG 
TATAATGCAT ATGAAGGATG TGCCAATGCA TTAACATCGC CTCCCAAGGT CATTAGGACT 
TTTTTCTATA CCAATCGCCA AACTTGTCAA GACTGGCTGA CTCGGATCCG ACTCTCCATC 
ATGAGGGTTG GATTGTTGGC AGGCCAGCCT GCTGTGACAG TGAGACACGG CTTCGATTTG 
CTTACAGAAA TGAAGACAAA TCCGTCTCAG GGAAATGAAT TGGAAGTAAC TATCATGATG 
GTGGTAGAAG CATTGTGTGA ACTTCATTGT CCTGAAGCTA TACAGGGAAT TGCTGTCTGG 
TCGTCTTCGG TTGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA 
GGGAGGTTTG AAAAGGCCTC TGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTC 
GATTGCTGCA TCTCTAGCTT CGACAAATCC GTTCTCACAT TGGCTAATAC TGGGCGTAAT 
AGTGCCAGCC CCAAGCATTC TTTGAATGGT GAATCCAGAA AAACTGTGCT TTCCAAACCA 
ACTGATTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT 
TCAATAGCTG ATTGGGCTGC TGTACAGGAG TGGCAGAATG CTGTCCATGA CTTGAAAAAG 
AGTACCAGTA GTACTTCTCT CAACCTAAAG GCTGATTTCA ACTACATAAA ATCACTAAGC 
AGTTTTGAAT CTGGAGAATT TATTGAATGC ACTGAACAAT TAGAATTGTT ACCAGGAGAA 
AATATCAGTC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTGCTTCCT 
AACATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TTGAAGTTCA GTTGTTAAGA 
AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CACATAGAAC AAGATCAGAA GTGGCAGTCT 
ATTACCGAAA ATGTGGTGAA GTACCTGAAG CAAACCTCCC GCATAGCTGT TGGACCCCTG 
AGACTTTCTA CTCTAACAGT TTCCCAGTCT CTGCCAGTCC TAAGTACTTT ACAGCTGTAT 
TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTG CCACAGAGGA CTGTCTTATT 
CCACTCTTTA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAA 
GCATTAAGAT ACACGATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT 
CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG 
GGGAATGTGT CACTTGCAAC AAGATTGCTG GCACAGTGTA GCGAGGTTCA GCTGGGAAAG 
ACTACCACTG CACAGGATTT AGTCCAGCAC TTTAAAAAAC TATCAACTCA AGGTCAAGTG 
GATGAAAAAT GGGGGCCTGA ACTTGACATT GAAAAAACGA AACTGCTATA TACAGCAGGC 
CAGTCAACAC ATGCAATGGA GATGTTGAGT TCCTGTGCCA TCTCTTTCTG CAAGTCTGCC 
AAAGCTGAGC ATGCTGTTGC CAAGTCAATT TTGACATTGG CTAAATGGAT CCAGGCAGAA 
TGGAAAGAAA TCTCAGGACA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAGAACTTC 
ACGGGTCTTT CTACTTTGTC TAAAAACATA CTCACTCTAA TTGAACTACC ATCTGTTAAT 
ACGATGGAGG AAGAGTATCC TCGGATAGAG AGTGAATCTA CAGTACATAT TGGAGTTGGG 
GAACCTGACT TCATCTTGGG ACAGCTGTAT CATCTGTCTT CAGTACAGGC ACCTGAAGTA 
GCCAAATCTT GGGCCGCATT GGCTAGTTGG GCTTACAGGT GGGGCAGAAA GGTGGTTGAC 
AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCTAGAG AAAAATCTGA AGTTCAGAAT 
CTGCTCCCAG ACACCATAAC CGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGTCAG 
GCTGTGTGTC GCCCAGCGGG GATTCAGGAT GAAGATATAA CGCTTCAGAT CACAGAAAGT 
GAAGATAATG AGGAAGATGA CATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCAAGTTGC 
CCATGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGAG TCATTAAAGT GTGGAGGAAA 
GTTGTGGACA GAATCTTCAG CCTGTATAAA CTGTCCTGCA GCGCATATTT TACTTTCCTT 
AAACTCAATG CTGGGCAGAT TCCTTTAGAT GAAGATGACC CTCGGCTCCA TTTAAGCCAC 
AGAGTGGAGC AGAGCACGGA TGACATGATT GTGATGGCCA CGCTGCGGTT GCTCAGGTTG 
CTCGTGAAGC ACGCTGGGGA GCTTCGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCT 
ACTGCGCCTT GGAGAGGAAT TATTCCGCAG CTTTTCTCAC GCTTAAACCA CCCTGAAGTG 
TATGTGCGCC AGAGTATTTG TAACCTCCTC TGCCGCGTGG CTCAGGATTC CCCGCATCTC 
ATACTGTATC CTGCAATAGT GGGCACCATA TCGCTTAGTA GTGAGTCACA GGCTTCAGGA 
AATAAATTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAACTACTG 
GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCTCAAG ATAGCAATAA GGATGAACCT 
AAAAGTGGAC TGAATGAAGA CCAAGCCATG ATGCAGGACT GCTACAGCAA AATTGTAGAT 
AAACTGTCCT CGGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTTGT GGCTGAGCTG 
CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG 
TATGTCCTGA GACGAATTCA GCAGTTGGAA GACGAGGTGA AGAGAGTCCA GAACAACAAC 
ACCTTACGCA AAGAAGAGAA AATCGCAATC ATGCGGGAGA AGCACACAGC TTTGATGAAG 
CCCATCGTAT TTGCTTTGGA GCACGTTAGG AGCATCACAG CGGCCCCTGC AGAAACACCT 
CATGAAAAGT GGTTTCAGGA TAACTATGGT GATGCCATTG AAAATGCTCT AGAAAAGCTG 
AAAACACCGT CAAACCCTGC AAAGCCTGGA AGCAGCTGGA TTCCATTTAA AGAGATAATG 
CTGAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGTTACA TCTTGCGTCT TGAAGAAATC 
AGCCCATGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCCGGAGA AGTCTCAGCC 
AGAGATACCG TCACAATCCA CAGTGTTGGT GGAACCATCA CAATCTTACC TACTAAAACC 
AAGCCAAAGA AACTTCTCTT TCTAGGATCA GATGGGAAGA GCTATCCTTA TCTTTTCAAA 
GGATTGGAGG ATTTACATCT GGATGAGAGA ATAATGCAAT TTCTGTCTAT TGTAAATACC 
ATGTTTGCAA CAATCAATCG CCAGGAAACA CCTCGTTTCC ATGCTCGCCA CTATTCTGTA 
ACACCACTGG GAACTCGCTC AGGCCTAATC CAGTGGGTGG ATGGAGCCAC GCCCTTATTT 
GGTCTTTACA AACGATGGCA ACAACGGGAA GCCGCCTTGC AAGCACAAAA GGCCCAAGAT 
TCCTACCAAA CTCCTCAGAA TCCTGGAATC GTACCCCGTC CTAGTGAACT TTATTATAGT 
AAAATTGGTC CTGCTTTGAA AGCAGTTGGG CTTAGTCTGG ATGTGTCCCG CCGGGATTGG 
CCTCTTCATG TGATGAAGGC AGTGTTAGAA GAGTTAATGG AGGCCACGCC CCCAAATCTC 
CTTGCTAAAG AGCTTTGGTC ATCATGTACC ACCCCTGATG AGTGGTGGAG AGTCACACAG 
TCTTATGCCA GATCTACTGC AGTCATGTCT ATGGTCGGAT ACATAATTGG CCTTGGAGAT 
AGACATCTGG ATAATGTTCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC 
AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTACC TTTTCGAATG 
ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGGCTTTCA 
TGTGAGCAGG TTCTACACAT TATGCGGCGT GGCAGAGAGA CTCTGCTGAC GCTGCTGGAG 
GCCTTTGTAT ATGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT 
GCGGTCTATG GTGGAGGGGG CCAGCAGGCT GAGAGCAAGC AGAGCAAGAG AGAGATGGAA 
CGAGAGATTA CTCGCAGCCT TTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT 
AAAAACAGAG ATGAGATGCT GGTTGTACTT CCCAAGCTGG ACAGCAGCTT AGATGAATAC 
CTGAGCTTGC AAGAGCAACT AACAGATGTG GAGAAACTGC AGGGCAAACT GCTGGAAGAA 
ATTGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG 
TATTCTGAAC ACACTCAACT ACAGACTCAG CAGAGAGCTG TTCAGGAAGC AATCCAGGTG 
AAGCTGAATG AATTTGAACA GTGGATAACA CACTATCAGG CTGCGTTCAA TAATTTGGAA 
GCTACACAGC TGGCCAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT TGGTCCTCCC 
AGCTACGTGC CGGCAACAGC CTTTCTGCAG AATGCTGGCC AGGCCCACTT GATTAGCCAG 
TGTGAGCAGC TAGAAGGGGA GGTTGGTGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA 
GGCTGTCTGG AGCAGCTGCA TCACTACGCA ACTGTTGCTC TGCAGTATCC AAAGGCCATA 
TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAGCTCAT CTGTAATACC 
ACAGTAGAAC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC GCCCCAGCCA 
CCCCCAACAG TGTGTCAGTT CATCACGGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCC 
GACATCAACA GCCGACTTAT CAGACAGGTG GAACGCTTGA AACAGGAAGC TGTCACTGTA 
CCGGTTTGTG AAGATCAGTT GAAAGAAATT GAACGTTGTA TCAAAGTTTT CCTTCACGAG 
AATGGAGAGG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TTTCTGCCCT TTGTACTCTC 
ACAAGGCGTA ACCTAATGAT GGAAGGTGCA GCGTCGAGTG CTGGAGAGCA GCTGGTTGAT 
CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAACTCT GCAGTATGAG CGGAAATGTC 
ACATGTCTGG TTCAGTTACT GAAGCAGTGT CATTTAGTGC CACAAGACTT AGACATTCCT 
AACCCCATGG AAGCATCTGA GGCCGTTCAT TTGGCTAATG GTGTATACAC CTCACTTCAG 
GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA 
GGAGAATACA CGTTAGAAAA TATGCTTCAT GAGCTGGACA GTCTTATTGA GCAGACAACT 
GATGGTGTTC CTCTGCAGAC TCTAGTTGAA TCTCTTCAAG CCTATTTAAG AAATGCAGCT 
ATGGGACTTG AGGAAGAAAC ACATGCTCAT TACATCGATG TTGCCAGAAT GCTTCATGCT 
CAGTATGGTG AATTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA 
GCTGGCCAGA TGCTTTTGGT AGCATTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT 
GGCTTATTGG TTGAAAAGCT AAATAAGATG GAAATTCCTA TAGCTTGGCG AAAGATTGAC 
ATAATACGGG AAGCCAGGAG CACTCAGGTC AATTTTTTTG ATGATGATAA TCACCGACAG 
GTGCTGGAAG AGATCTTCTT TCTAAAACGA CTACAGACAA TTAAGGAGTT CTTTCGGCTC 
TGTGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA 
AATGGGCCTG TACAGATTGT CAATGTGAAA ACCCTTTTTA GAAACTCTTG TTTCAGTGAA 
GACCAGATGG CCAAACCAAT AAAGGCATTC ACAGCTGACT TTGTGCGGCA GCTCCTGATT 
GGACTCCCGA ACCAAGCCCT GGGCCTCACA CTGTGCAGCT TCATCAGTGC TCTAGGGGTA 
GACATCATTG CGCAGGTGGA GGCGAAGGAT TTTGGTGCTG AAAGCAAAGT TTCTGTTGAC 
GATCTGTGTA AGAAGGCGGT GGAGCACAAC ATCCAGATCG GGAAGTTCTC TCAGCTGGTC 
ATGAACAGGG CGACTGTGCT GGCGAGCTCC TACGACACTG CCTGGAAGAA GCACGACTTG 
GTGCGCAGGC TGGAGACCAG CATCGCCTCC TGCAAGACAA GCCTGCAGCG TGTCCAGCTG 
CACATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAATAGACC ACAAGCCATG 
TCTGTCACAC CTCCCCCTCG TTCTGCCATA TTAACCAGCA TGAAAAAGAA ACTCCATACT 
CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC ACTTGAAGCA 
AGTATTGAAC AGCGACTCAA ATGGGCAGGT GGTGCCAACC CTGCACTGGC ACCTGTACTA 
CAGGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAGGA GAGCCAAAGA 
GCAAGTCAGG TCACTTTCCT CTGCAGCAAT ATCATTCATT TTGAAAGTTT ACGAACTAGA 
ACTGCAGAAG CCTTAAACCT GGATGCTGCA TTATTTGAAC TAATCAAACG GTGTCAGCAA 
ATGTGTTCAT TTGCATCGCA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA 
CAGAGGGTGG ACACGACTCT TGAACATCCT ATTGGCAGCT CTGAGTGGCT TTTGTCAGCA 
CACAAACAAC TGACCCAGGA TATGTCTACT CAGAGAGCAA TTCAGACAGA GAAAGAGCAA 
CAAATAGAAA CAGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC 
ACTGGCCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT 
GAAGAAGCTG CTCTGGCAGA TGGTGAAGAT GTTCCCTATG AGAACAGTGT TAGGCAGTTC 
TTGGGTGAAT ATAAATCATG GCAAGACAAC ATTCAGACAG TGCTGTTTAC ATTAGTCCAA 
GCCATGGGTC AGGTTCGAAG TCAAGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC 
TTGAAAGAAC TGAAAACGCA AAGTCAGAGT GTGTATAATA ATTTAGTGAG TTTTGCATCA 
CCATTAGTGA CCGATGCAAC AAATGAATGT TCGAGTCCAA CATCGTCTGC TACTTATCAA 
CCATCCTTTG CTGCAGCAGT CCGAAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG 
TCACAGAACG CTAGAAAGCT GATTCAGAAA AACCTAGCTA CATCAGCAGA TACCCCACCG 
AGCACCGTTC CGGGAACTGG CAAGAGTGTT GCTTGTAGTC CTAAAAAGGC CATCAGAGAC 
CCTAAGACTG GAAAAGCTGT GCAGGAGAGA AACTCGTATG CGGTGAGTGT GTGGAAGAGA 
GTGAAAGCCA AGCTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA 
CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA 
GGTTGGACAG CCTGGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224801.1
CDS	114..11054
Translation	MQKHHKGSILYSMRFLRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRIHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSIQHGSRALAIKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRIGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNPSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSVVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANTGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFIECTEQLELLPGENISLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSITENVVKYLKQTSRIAVGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLATEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQSQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEHAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLENMLHELDSLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSIASCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005224744.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224744.3

ORF Insert Sequence:

ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
GCATCAGCCG ACCCAGATAA TTTAAAATAC TCTTCATCCA GAGATAGGGG TGGGTCTTCC 
TCTTATGGAC TGCAGCCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA TGATGATACC 
AGAATCCATG CTGACATACA GAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT 
GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC 
AGCCCTGAGT TTACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG 
AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG 
AGGATCACCC GGGAAGATGA CAGAGACCGA AGGCTGGCTA CTGTTAAGCA GTTGAAAGAA 
TTTATTCAGC AACCAGAAAA TAAGCTGGTG CTAGTTAAGC AGTTGGATAA TATCTTGGCT 
GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA 
GCTTGTTGTC TTGGCCTTCT TTGTGCATCT CTGAGCTATG AGGCTGAGAA GATCTTCAAA 
TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TTAAACTCCT CTACTTATGT 
GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG 
CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC 
AAGTGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACAAAT 
TTTAGGGATA CAGTTGATAT ATTAGTTGGA TGGCATATAG ATCATACTCA GAAGCCTTCA 
CTTACACAAC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGACCTT 
GCATTTTCTA CCACTCTTCT TGGTCAGTTT TTAGAGGACA TGGAGGCATA TGCTGAGGAC 
CTCAGCCATG TAGCCTCTGG GGAGTCAGTG GATGAAGATG TTCCTCCTCC ATCAGTGTCC 
TTACCAAAGC TGGCAGCACT TCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA 
CGTTTCAGCC CAATTCGAGG TCCTCCAATT ACTGAGGCAT ATGTAACAGA TGTTCTGTAC 
AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAAGTGTTTT TTTCTGAGGC TGTGTTGACA 
GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT 
TGTGACATGG TTATTACATA TGGATTAGAT CAACTGGAGA ATTGCCAGAC TTGTGGTACA 
GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA 
CTGCCATCAT CATTTGTAGA AAAACTGTTT ATACCATCAT CTAAGCTACT ATTTTTGCGT 
TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGT 
CTGAAGAATA TTCCTGTTTT AGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT 
GCCCTAAACA ATCTGCTACA TAGTCTACAA CTTCCTGATG CCTGTTCTGA AATAAAACAT 
GAGGCTTTTA AGAATCATGT TTTCAATGTA GACAATGCAA AATTTGTAGT TATATTTGAC 
CTTAGTGCTC TGACTACAAT TGGAAATGCT AAAAACTCAC TAATTGGGAT GTGGGCCCTG 
TCTCCAACTG TGTTTGCACT TCTGAGTAAG AATCTGATGA TTGTGCATGG CGACCTGGCT 
GTTCATTTCC CTGCCATTCA GTATGCAGTG CTCTACACAT TGTATTCTCA TTGTACCAGG 
CATGACCACT TTATATCTAG TAGTCTTAGT TCATCATCCC CGTCCTTGTT TGATGGTGCC 
GTTATTAGTA CTGTAACTAC AGCTACAAAA AAACATTTCT CAATTATCTT AAATCTCCTG 
GGAATATTGC TTAAGAAAGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG 
GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ATGCACCTTT ATTCTCTCTT 
CCATCTTTCC ATAAATTTTG CAAAGGCCTT TTAGCCAACA CTCTGGTCGA AGATGTGAAT 
ATCTGTCTGC AAGCATGCAG CAGTCTACAT GCACTGTCCT CTTCCTTACC AGATGATCTT 
TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAGCTAGTCC ATAGTGGAAC TCGTATTCGA 
CAAGCATTTG GAAAGCTATT GAAGTCAATC CCTTTAGATG TTGTCTTGAG CAATAACAAT 
CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT 
AACACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGAAACTCT 
CATAGAATAG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ATAGCTGCCA GAGACTGGAT 
AAACGTGACC AATCAACGAT TCCCCGCAAT CTGCTAAAGA CAGATGCTGT CCTCTGGCAG 
TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGCTGAGGAC CCCACTTGGC 
AGAGCTCAGG ATACCTTCCA AACAATTGAA GGTATCATTA GAAGTCTTGC AGCTCACACA 
TTAAACCCCG ATCAAGATGT TAGTCAGTGG ACAACTGCAG ACAATGACGA AGGCCACAGT 
AGCAACCAGC TTAGACTTGT GCTGCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG 
TATAATGCAT ATGAAGGATG TGCCAATGCA TTAACATCGC CTCCCAAGGT CATTAGGACT 
TTTTTCTATA CCAATCGCCA AACTTGTCAA GACTGGCTGA CTCGGATCCG ACTCTCCATC 
ATGAGGGTTG GATTGTTGGC AGGCCAGCCT GCTGTGACAG TGAGACACGG CTTCGATTTG 
CTTACAGAAA TGAAGACAAA TCCGTCTCAG GGAAATGAAT TGGAAGTAAC TATCATGATG 
GTGGTAGAAG CATTGTGTGA ACTTCATTGT CCTGAAGCTA TACAGGGAAT TGCTGTCTGG 
TCGTCTTCGG TTGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA 
GGGAGGTTTG AAAAGGCCTC TGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTC 
GATTGCTGCA TCTCTAGCTT CGACAAATCC GTTCTCACAT TGGCTAATAC TGGGCGTAAT 
AGTGCCAGCC CCAAGCATTC TTTGAATGGT GAATCCAGAA AAACTGTGCT TTCCAAACCA 
ACTGATTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT 
TCAATAGCTG ATTGGGCTGC TGTACAGGAG TGGCAGAATG CTGTCCATGA CTTGAAAAAG 
AGTACCAGTA GTACTTCTCT CAACCTAAAG GCTGATTTCA ACTACATAAA ATCACTAAGC 
AGTTTTGAAT CTGGAGAATT TATTGAATGC ACTGAACAAT TAGAATTGTT ACCAGGAGAA 
AATATCAGTC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTGCTTCCT 
AACATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TTGAAGTTCA GTTGTTAAGA 
AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CACATAGAAC AAGATCAGAA GTGGCAGTCT 
ATTACCGAAA ATGTGGTGAA GTACCTGAAG CAAACCTCCC GCATAGCTGT TGGACCCCTG 
AGACTTTCTA CTCTAACAGT TTCCCAGTCT CTGCCAGTCC TAAGTACTTT ACAGCTGTAT 
TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTG CCACAGAGGA CTGTCTTATT 
CCACTCTTTA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAA 
GCATTAAGAT ACACGATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT 
CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG 
GGGAATGTGT CACTTGCAAC AAGATTGCTG GCACAGTGTA GCGAGGTTCA GCTGGGAAAG 
ACTACCACTG CACAGGATTT AGTCCAGCAC TTTAAAAAAC TATCAACTCA AGGTCAAGTG 
GATGAAAAAT GGGGGCCTGA ACTTGACATT GAAAAAACGA AACTGCTATA TACAGCAGGC 
CAGTCAACAC ATGCAATGGA GATGTTGAGT TCCTGTGCCA TCTCTTTCTG CAAGTCTGCC 
AAAGCTGAGC ATGCTGTTGC CAAGTCAATT TTGACATTGG CTAAATGGAT CCAGGCAGAA 
TGGAAAGAAA TCTCAGGACA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAGAACTTC 
ACGGGTCTTT CTACTTTGTC TAAAAACATA CTCACTCTAA TTGAACTACC ATCTGTTAAT 
ACGATGGAGG AAGAGTATCC TCGGATAGAG AGTGAATCTA CAGTACATAT TGGAGTTGGG 
GAACCTGACT TCATCTTGGG ACAGCTGTAT CATCTGTCTT CAGTACAGGC ACCTGAAGTA 
GCCAAATCTT GGGCCGCATT GGCTAGTTGG GCTTACAGGT GGGGCAGAAA GGTGGTTGAC 
AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCTAGAG AAAAATCTGA AGTTCAGAAT 
CTGCTCCCAG ACACCATAAC CGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGTCAG 
GCTGTGTGTC GCCCAGCGGG GATTCAGGAT GAAGATATAA CGCTTCAGAT CACAGAAAGT 
GAAGATAATG AGGAAGATGA CATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCAAGTTGC 
CCATGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGAG TCATTAAAGT GTGGAGGAAA 
GTTGTGGACA GAATCTTCAG CCTGTATAAA CTGTCCTGCA GCGCATATTT TACTTTCCTT 
AAACTCAATG CTGGGCAGAT TCCTTTAGAT GAAGATGACC CTCGGCTCCA TTTAAGCCAC 
AGAGTGGAGC AGAGCACGGA TGACATGATT GTGATGGCCA CGCTGCGGTT GCTCAGGTTG 
CTCGTGAAGC ACGCTGGGGA GCTTCGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCT 
ACTGCGCCTT GGAGAGGAAT TATTCCGCAG CTTTTCTCAC GCTTAAACCA CCCTGAAGTG 
TATGTGCGCC AGAGTATTTG TAACCTCCTC TGCCGCGTGG CTCAGGATTC CCCGCATCTC 
ATACTGTATC CTGCAATAGT GGGCACCATA TCGCTTAGTA GTGAGTCACA GGCTTCAGGA 
AATAAATTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAACTACTG 
GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCTCAAG ATAGCAATAA GGATGAACCT 
AAAAGTGGAC TGAATGAAGA CCAAGCCATG ATGCAGGACT GCTACAGCAA AATTGTAGAT 
AAACTGTCCT CGGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTTGT GGCTGAGCTG 
CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG 
TATGTCCTGA GACGAATTCA GCAGTTGGAA GACGAGGTGA AGAGAGTCCA GAACAACAAC 
ACCTTACGCA AAGAAGAGAA AATCGCAATC ATGCGGGAGA AGCACACAGC TTTGATGAAG 
CCCATCGTAT TTGCTTTGGA GCACGTTAGG AGCATCACAG CGGCCCCTGC AGAAACACCT 
CATGAAAAGT GGTTTCAGGA TAACTATGGT GATGCCATTG AAAATGCTCT AGAAAAGCTG 
AAAACACCGT CAAACCCTGC AAAGCCTGGA AGCAGCTGGA TTCCATTTAA AGAGATAATG 
CTGAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGTTACA TCTTGCGTCT TGAAGAAATC 
AGCCCATGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCCGGAGA AGTCTCAGCC 
AGAGATACCG TCACAATCCA CAGTGTTGGT GGAACCATCA CAATCTTACC TACTAAAACC 
AAGCCAAAGA AACTTCTCTT TCTAGGATCA GATGGGAAGA GCTATCCTTA TCTTTTCAAA 
GGATTGGAGG ATTTACATCT GGATGAGAGA ATAATGCAAT TTCTGTCTAT TGTAAATACC 
ATGTTTGCAA CAATCAATCG CCAGGAAACA CCTCGTTTCC ATGCTCGCCA CTATTCTGTA 
ACACCACTGG GAACTCGCTC AGGCCTAATC CAGTGGGTGG ATGGAGCCAC GCCCTTATTT 
GGTCTTTACA AACGATGGCA ACAACGGGAA GCCGCCTTGC AAGCACAAAA GGCCCAAGAT 
TCCTACCAAA CTCCTCAGAA TCCTGGAATC GTACCCCGTC CTAGTGAACT TTATTATAGT 
AAAATTGGTC CTGCTTTGAA AGCAGTTGGG CTTAGTCTGG ATGTGTCCCG CCGGGATTGG 
CCTCTTCATG TGATGAAGGC AGTGTTAGAA GAGTTAATGG AGGCCACGCC CCCAAATCTC 
CTTGCTAAAG AGCTTTGGTC ATCATGTACC ACCCCTGATG AGTGGTGGAG AGTCACACAG 
TCTTATGCCA GATCTACTGC AGTCATGTCT ATGGTCGGAT ACATAATTGG CCTTGGAGAT 
AGACATCTGG ATAATGTTCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC 
AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTACC TTTTCGAATG 
ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGGCTTTCA 
TGTGAGCAGG TTCTACACAT TATGCGGCGT GGCAGAGAGA CTCTGCTGAC GCTGCTGGAG 
GCCTTTGTAT ATGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT 
GCGGTCTATG GTGGAGGGGG CCAGCAGGCT GAGAGCAAGC AGAGCAAGAG AGAGATGGAA 
CGAGAGATTA CTCGCAGCCT TTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT 
AAAAACAGAG ATGAGATGCT GGTTGTACTT CCCAAGCTGG ACAGCAGCTT AGATGAATAC 
CTGAGCTTGC AAGAGCAACT AACAGATGTG GAGAAACTGC AGGGCAAACT GCTGGAAGAA 
ATTGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG 
TATTCTGAAC ACACTCAACT ACAGACTCAG CAGAGAGCTG TTCAGGAAGC AATCCAGGTG 
AAGCTGAATG AATTTGAACA GTGGATAACA CACTATCAGG CTGCGTTCAA TAATTTGGAA 
GCTACACAGC TGGCCAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT TGGTCCTCCC 
AGCTACGTGC CGGCAACAGC CTTTCTGCAG AATGCTGGCC AGGCCCACTT GATTAGCCAG 
TGTGAGCAGC TAGAAGGGGA GGTTGGTGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA 
GGCTGTCTGG AGCAGCTGCA TCACTACGCA ACTGTTGCTC TGCAGTATCC AAAGGCCATA 
TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAGCTCAT CTGTAATACC 
ACAGTAGAAC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC GCCCCAGCCA 
CCCCCAACAG TGTGTCAGTT CATCACGGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCC 
GACATCAACA GCCGACTTAT CAGACAGGTG GAACGCTTGA AACAGGAAGC TGTCACTGTA 
CCGGTTTGTG AAGATCAGTT GAAAGAAATT GAACGTTGTA TCAAAGTTTT CCTTCACGAG 
AATGGAGAGG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TTTCTGCCCT TTGTACTCTC 
ACAAGGCGTA ACCTAATGAT GGAAGGTGCA GCGTCGAGTG CTGGAGAGCA GCTGGTTGAT 
CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAACTCT GCAGTATGAG CGGAAATGTC 
ACATGTCTGG TTCAGTTACT GAAGCAGTGT CATTTAGTGC CACAAGACTT AGACATTCCT 
AACCCCATGG AAGCATCTGA GGCCGTTCAT TTGGCTAATG GTGTATACAC CTCACTTCAG 
GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA 
GGAGAATACA CGTTAGAAAA TATGCTTCAT GAGCTGGACA GTCTTATTGA GCAGACAACT 
GATGGTGTTC CTCTGCAGAC TCTAGTTGAA TCTCTTCAAG CCTATTTAAG AAATGCAGCT 
ATGGGACTTG AGGAAGAAAC ACATGCTCAT TACATCGATG TTGCCAGAAT GCTTCATGCT 
CAGTATGGTG AATTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA 
GCTGGCCAGA TGCTTTTGGT AGCATTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT 
GGCTTATTGG TTGAAAAGCT AAATAAGATG GAAATTCCTA TAGCTTGGCG AAAGATTGAC 
ATAATACGGG AAGCCAGGAG CACTCAGGTC AATTTTTTTG ATGATGATAA TCACCGACAG 
GTGCTGGAAG AGATCTTCTT TCTAAAACGA CTACAGACAA TTAAGGAGTT CTTTCGGCTC 
TGTGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA 
AATGGGCCTG TACAGATTGT CAATGTGAAA ACCCTTTTTA GAAACTCTTG TTTCAGTGAA 
GACCAGATGG CCAAACCAAT AAAGGCATTC ACAGCTGACT TTGTGCGGCA GCTCCTGATT 
GGACTCCCGA ACCAAGCCCT GGGCCTCACA CTGTGCAGCT TCATCAGTGC TCTAGGGGTA 
GACATCATTG CGCAGGTGGA GGCGAAGGAT TTTGGTGCTG AAAGCAAAGT TTCTGTTGAC 
GATCTGTGTA AGAAGGCGGT GGAGCACAAC ATCCAGATCG GGAAGTTCTC TCAGCTGGTC 
ATGAACAGGG CGACTGTGCT GGCGAGCTCC TACGACACTG CCTGGAAGAA GCACGACTTG 
GTGCGCAGGC TGGAGACCAG CATCGCCTCC TGCAAGACAA GCCTGCAGCG TGTCCAGCTG 
CACATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAATAGACC ACAAGCCATG 
TCTGTCACAC CTCCCCCTCG TTCTGCCATA TTAACCAGCA TGAAAAAGAA ACTCCATACT 
CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC ACTTGAAGCA 
AGTATTGAAC AGCGACTCAA ATGGGCAGGT GGTGCCAACC CTGCACTGGC ACCTGTACTA 
CAGGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAGGA GAGCCAAAGA 
GCAAGTCAGG TCACTTTCCT CTGCAGCAAT ATCATTCATT TTGAAAGTTT ACGAACTAGA 
ACTGCAGAAG CCTTAAACCT GGATGCTGCA TTATTTGAAC TAATCAAACG GTGTCAGCAA 
ATGTGTTCAT TTGCATCGCA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA 
CAGAGGGTGG ACACGACTCT TGAACATCCT ATTGGCAGCT CTGAGTGGCT TTTGTCAGCA 
CACAAACAAC TGACCCAGGA TATGTCTACT CAGAGAGCAA TTCAGACAGA GAAAGAGCAA 
CAAATAGAAA CAGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC 
ACTGGCCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT 
GAAGAAGCTG CTCTGGCAGA TGGTGAAGAT GTTCCCTATG AGAACAGTGT TAGGCAGTTC 
TTGGGTGAAT ATAAATCATG GCAAGACAAC ATTCAGACAG TGCTGTTTAC ATTAGTCCAA 
GCCATGGGTC AGGTTCGAAG TCAAGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC 
TTGAAAGAAC TGAAAACGCA AAGTCAGAGT GTGTATAATA ATTTAGTGAG TTTTGCATCA 
CCATTAGTGA CCGATGCAAC AAATGAATGT TCGAGTCCAA CATCGTCTGC TACTTATCAA 
CCATCCTTTG CTGCAGCAGT CCGAAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG 
TCACAGAACG CTAGAAAGCT GATTCAGAAA AACCTAGCTA CATCAGCAGA TACCCCACCG 
AGCACCGTTC CGGGAACTGG CAAGAGTGTT GCTTGTAGTC CTAAAAAGGC CATCAGAGAC 
CCTAAGACTG GAAAAGCTGT GCAGGAGAGA AACTCGTATG CGGTGAGTGT GTGGAAGAGA 
GTGAAAGCCA AGCTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA 
CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA 
GGTTGGACAG CCTGGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005224745.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224802.1 Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X2, mRNA.

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CloneID	OBa32979
Clone ID Related Accession (Same CDS sequence)	XM_005224745.3 , XM_005224745.4
Accession Version	XM_005224745.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCC TGTCTCCAAC TGTGTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAT 
GGCGACCTGG CTGTTCATTT CCCTGCCATT CAGTATGCAG TGCTCTACAC ATTGTATTCT 
CATTGTACCA GGCATGACCA CTTTATATCT AGTAGTCTTA GTTCATCATC CCCGTCCTTG 
TTTGATGGTG CCGTTATTAG TACTGTAACT ACAGCTACAA AAAAACATTT CTCAATTATC 
TTAAATCTCC TGGGAATATT GCTTAAGAAA GATAATCTTA ACCAGGACAC AAGGAAACTG 
TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATATGCACCT 
TTATTCTCTC TTCCATCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTGGTC 
GAAGATGTGA ATATCTGTCT GCAAGCATGC AGCAGTCTAC ATGCACTGTC CTCTTCCTTA 
CCAGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAGCTAGT CCATAGTGGA 
ACTCGTATTC GACAAGCATT TGGAAAGCTA TTGAAGTCAA TCCCTTTAGA TGTTGTCTTG 
AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT 
AAAGCACCAA GTAACACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG 
TATGGAAACT CTCATAGAAT AGGGAAGGAC AATTGGTTAG AAAGATTGTT CTATAGCTGC 
CAGAGACTGG ATAAACGTGA CCAATCAACG ATTCCCCGCA ATCTGCTAAA GACAGATGCT 
GTCCTCTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGCTGAGG 
ACCCCACTTG GCAGAGCTCA GGATACCTTC CAAACAATTG AAGGTATCAT TAGAAGTCTT 
GCAGCTCACA CATTAAACCC CGATCAAGAT GTTAGTCAGT GGACAACTGC AGACAATGAC 
GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTGCTTC TGCAGTATCT GGAAAACCTG 
GAGAAGTTAA TGTATAATGC ATATGAAGGA TGTGCCAATG CATTAACATC GCCTCCCAAG 
GTCATTAGGA CTTTTTTCTA TACCAATCGC CAAACTTGTC AAGACTGGCT GACTCGGATC 
CGACTCTCCA TCATGAGGGT TGGATTGTTG GCAGGCCAGC CTGCTGTGAC AGTGAGACAC 
GGCTTCGATT TGCTTACAGA AATGAAGACA AATCCGTCTC AGGGAAATGA ATTGGAAGTA 
ACTATCATGA TGGTGGTAGA AGCATTGTGT GAACTTCATT GTCCTGAAGC TATACAGGGA 
ATTGCTGTCT GGTCGTCTTC GGTTGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC 
CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCTGTGGAGT ACCAGGAGCA CCTGTGTGCC 
ATGACAGGTG TCGATTGCTG CATCTCTAGC TTCGACAAAT CCGTTCTCAC ATTGGCTAAT 
ACTGGGCGTA ATAGTGCCAG CCCCAAGCAT TCTTTGAATG GTGAATCCAG AAAAACTGTG 
CTTTCCAAAC CAACTGATTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT 
GAGTGCTACA TTTCAATAGC TGATTGGGCT GCTGTACAGG AGTGGCAGAA TGCTGTCCAT 
GACTTGAAAA AGAGTACCAG TAGTACTTCT CTCAACCTAA AGGCTGATTT CAACTACATA 
AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTATTGAAT GCACTGAACA ATTAGAATTG 
TTACCAGGAG AAAATATCAG TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA 
AAACTGCTTC CTAACATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATTGAAGTT 
CAGTTGTTAA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCACATAGA ACAAGATCAG 
AAGTGGCAGT CTATTACCGA AAATGTGGTG AAGTACCTGA AGCAAACCTC CCGCATAGCT 
GTTGGACCCC TGAGACTTTC TACTCTAACA GTTTCCCAGT CTCTGCCAGT CCTAAGTACT 
TTACAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TGCCACAGAG 
GACTGTCTTA TTCCACTCTT TAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG 
CCATGGATGC AAGCATTAAG ATACACGATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA 
GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT 
GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGATTGC TGGCACAGTG TAGCGAGGTT 
CAGCTGGGAA AGACTACCAC TGCACAGGAT TTAGTCCAGC ACTTTAAAAA ACTATCAACT 
CAAGGTCAAG TGGATGAAAA ATGGGGGCCT GAACTTGACA TTGAAAAAAC GAAACTGCTA 
TATACAGCAG GCCAGTCAAC ACATGCAATG GAGATGTTGA GTTCCTGTGC CATCTCTTTC 
TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCAA TTTTGACATT GGCTAAATGG 
ATCCAGGCAG AATGGAAAGA AATCTCAGGA CAGCTGAAAC AGGTTTACAG AGCTCAGCAG 
CAGCAGAACT TCACGGGTCT TTCTACTTTG TCTAAAAACA TACTCACTCT AATTGAACTA 
CCATCTGTTA ATACGATGGA GGAAGAGTAT CCTCGGATAG AGAGTGAATC TACAGTACAT 
ATTGGAGTTG GGGAACCTGA CTTCATCTTG GGACAGCTGT ATCATCTGTC TTCAGTACAG 
GCACCTGAAG TAGCCAAATC TTGGGCCGCA TTGGCTAGTT GGGCTTACAG GTGGGGCAGA 
AAGGTGGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCTAG AGAAAAATCT 
GAAGTTCAGA ATCTGCTCCC AGACACCATA ACCGAAGAAG AAAAAGAGAG AATATATGGT 
ATTCTTGGTC AGGCTGTGTG TCGCCCAGCG GGGATTCAGG ATGAAGATAT AACGCTTCAG 
ATCACAGAAA GTGAAGATAA TGAGGAAGAT GACATGGTTG ATGTTATCTG GCGTCAGTTA 
ATATCAAGTT GCCCATGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG AGTCATTAAA 
GTGTGGAGGA AAGTTGTGGA CAGAATCTTC AGCCTGTATA AACTGTCCTG CAGCGCATAT 
TTTACTTTCC TTAAACTCAA TGCTGGGCAG ATTCCTTTAG ATGAAGATGA CCCTCGGCTC 
CATTTAAGCC ACAGAGTGGA GCAGAGCACG GATGACATGA TTGTGATGGC CACGCTGCGG 
TTGCTCAGGT TGCTCGTGAA GCACGCTGGG GAGCTTCGGC AGTATCTGGA ACATGGCTTG 
GAAACAACAC CTACTGCGCC TTGGAGAGGA ATTATTCCGC AGCTTTTCTC ACGCTTAAAC 
CACCCTGAAG TGTATGTGCG CCAGAGTATT TGTAACCTCC TCTGCCGCGT GGCTCAGGAT 
TCCCCGCATC TCATACTGTA TCCTGCAATA GTGGGCACCA TATCGCTTAG TAGTGAGTCA 
CAGGCTTCAG GAAATAAATT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA 
GAAGAACTAC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCTCA AGATAGCAAT 
AAGGATGAAC CTAAAAGTGG ACTGAATGAA GACCAAGCCA TGATGCAGGA CTGCTACAGC 
AAAATTGTAG ATAAACTGTC CTCGGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTT 
GTGGCTGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA 
CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGTTGG AAGACGAGGT GAAGAGAGTC 
CAGAACAACA ACACCTTACG CAAAGAAGAG AAAATCGCAA TCATGCGGGA GAAGCACACA 
GCTTTGATGA AGCCCATCGT ATTTGCTTTG GAGCACGTTA GGAGCATCAC AGCGGCCCCT 
GCAGAAACAC CTCATGAAAA GTGGTTTCAG GATAACTATG GTGATGCCAT TGAAAATGCT 
CTAGAAAAGC TGAAAACACC GTCAAACCCT GCAAAGCCTG GAAGCAGCTG GATTCCATTT 
AAAGAGATAA TGCTGAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGTTA CATCTTGCGT 
CTTGAAGAAA TCAGCCCATG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCCGGA 
GAAGTCTCAG CCAGAGATAC CGTCACAATC CACAGTGTTG GTGGAACCAT CACAATCTTA 
CCTACTAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CAGATGGGAA GAGCTATCCT 
TATCTTTTCA AAGGATTGGA GGATTTACAT CTGGATGAGA GAATAATGCA ATTTCTGTCT 
ATTGTAAATA CCATGTTTGC AACAATCAAT CGCCAGGAAA CACCTCGTTT CCATGCTCGC 
CACTATTCTG TAACACCACT GGGAACTCGC TCAGGCCTAA TCCAGTGGGT GGATGGAGCC 
ACGCCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCCGCCTT GCAAGCACAA 
AAGGCCCAAG ATTCCTACCA AACTCCTCAG AATCCTGGAA TCGTACCCCG TCCTAGTGAA 
CTTTATTATA GTAAAATTGG TCCTGCTTTG AAAGCAGTTG GGCTTAGTCT GGATGTGTCC 
CGCCGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGTTAG AAGAGTTAAT GGAGGCCACG 
CCCCCAAATC TCCTTGCTAA AGAGCTTTGG TCATCATGTA CCACCCCTGA TGAGTGGTGG 
AGAGTCACAC AGTCTTATGC CAGATCTACT GCAGTCATGT CTATGGTCGG ATACATAATT 
GGCCTTGGAG ATAGACATCT GGATAATGTT CTCATAGATA TGACGACTGG AGAGGTTGTT 
CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTA 
CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT 
TTTAGGCTTT CATGTGAGCA GGTTCTACAC ATTATGCGGC GTGGCAGAGA GACTCTGCTG 
ACGCTGCTGG AGGCCTTTGT ATATGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT 
GGGTTTGCTG GTGCGGTCTA TGGTGGAGGG GGCCAGCAGG CTGAGAGCAA GCAGAGCAAG 
AGAGAGATGG AACGAGAGAT TACTCGCAGC CTTTTTTCTT CGAGAGTAGC TGAAATTAAG 
GTGAACTGGT TTAAAAACAG AGATGAGATG CTGGTTGTAC TTCCCAAGCT GGACAGCAGC 
TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTAACAGATG TGGAGAAACT GCAGGGCAAA 
CTGCTGGAAG AAATTGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT 
CTGCAACACA GGTATTCTGA ACACACTCAA CTACAGACTC AGCAGAGAGC TGTTCAGGAA 
GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACACTATCA GGCTGCGTTC 
AATAATTTGG AAGCTACACA GCTGGCCAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC 
CTTGGTCCTC CCAGCTACGT GCCGGCAACA GCCTTTCTGC AGAATGCTGG CCAGGCCCAC 
TTGATTAGCC AGTGTGAGCA GCTAGAAGGG GAGGTTGGTG CCCTCCTGCA GCAGAGGCGC 
TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTACG CAACTGTTGC TCTGCAGTAT 
CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAGCTC 
ATCTGTAATA CCACAGTAGA ACGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT 
GCGCCCCAGC CACCCCCAAC AGTGTGTCAG TTCATCACGG CCACTGAGAT GACCCTGCAG 
CGGTACGCAG CCGACATCAA CAGCCGACTT ATCAGACAGG TGGAACGCTT GAAACAGGAA 
GCTGTCACTG TACCGGTTTG TGAAGATCAG TTGAAAGAAA TTGAACGTTG TATCAAAGTT 
TTCCTTCACG AGAATGGAGA GGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATTTCTGCC 
CTTTGTACTC TCACAAGGCG TAACCTAATG ATGGAAGGTG CAGCGTCGAG TGCTGGAGAG 
CAGCTGGTTG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAACT CTGCAGTATG 
AGCGGAAATG TCACATGTCT GGTTCAGTTA CTGAAGCAGT GTCATTTAGT GCCACAAGAC 
TTAGACATTC CTAACCCCAT GGAAGCATCT GAGGCCGTTC ATTTGGCTAA TGGTGTATAC 
ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA 
TGTTTAATGA AAGGAGAATA CACGTTAGAA AATATGCTTC ATGAGCTGGA CAGTCTTATT 
GAGCAGACAA CTGATGGTGT TCCTCTGCAG ACTCTAGTTG AATCTCTTCA AGCCTATTTA 
AGAAATGCAG CTATGGGACT TGAGGAAGAA ACACATGCTC ATTACATCGA TGTTGCCAGA 
ATGCTTCATG CTCAGTATGG TGAATTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA 
CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCATTTG ATGGCATGTT TGCCCAAGTT 
GAGACTGCTT TTGGCTTATT GGTTGAAAAG CTAAATAAGA TGGAAATTCC TATAGCTTGG 
CGAAAGATTG ACATAATACG GGAAGCCAGG AGCACTCAGG TCAATTTTTT TGATGATGAT 
AATCACCGAC AGGTGCTGGA AGAGATCTTC TTTCTAAAAC GACTACAGAC AATTAAGGAG 
TTCTTTCGGC TCTGTGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT 
CAGAATACTG TAAATGGGCC TGTACAGATT GTCAATGTGA AAACCCTTTT TAGAAACTCT 
TGTTTCAGTG AAGACCAGAT GGCCAAACCA ATAAAGGCAT TCACAGCTGA CTTTGTGCGG 
CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CACTGTGCAG CTTCATCAGT 
GCTCTAGGGG TAGACATCAT TGCGCAGGTG GAGGCGAAGG ATTTTGGTGC TGAAAGCAAA 
GTTTCTGTTG ACGATCTGTG TAAGAAGGCG GTGGAGCACA ACATCCAGAT CGGGAAGTTC 
TCTCAGCTGG TCATGAACAG GGCGACTGTG CTGGCGAGCT CCTACGACAC TGCCTGGAAG 
AAGCACGACT TGGTGCGCAG GCTGGAGACC AGCATCGCCT CCTGCAAGAC AAGCCTGCAG 
CGTGTCCAGC TGCACATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAATAGA 
CCACAAGCCA TGTCTGTCAC ACCTCCCCCT CGTTCTGCCA TATTAACCAG CATGAAAAAG 
AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA 
GCACTTGAAG CAAGTATTGA ACAGCGACTC AAATGGGCAG GTGGTGCCAA CCCTGCACTG 
GCACCTGTAC TACAGGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAG 
GAGAGCCAAA GAGCAAGTCA GGTCACTTTC CTCTGCAGCA ATATCATTCA TTTTGAAAGT 
TTACGAACTA GAACTGCAGA AGCCTTAAAC CTGGATGCTG CATTATTTGA ACTAATCAAA 
CGGTGTCAGC AAATGTGTTC ATTTGCATCG CAGTTTAACA GTTCAGTCTC TGAGTTAGAG 
CTTCGTTTAT TACAGAGGGT GGACACGACT CTTGAACATC CTATTGGCAG CTCTGAGTGG 
CTTTTGTCAG CACACAAACA ACTGACCCAG GATATGTCTA CTCAGAGAGC AATTCAGACA 
GAGAAAGAGC AACAAATAGA AACAGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA 
AAGACTGTGC TCACTGGCCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA 
ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGTGAAG ATGTTCCCTA TGAGAACAGT 
GTTAGGCAGT TCTTGGGTGA ATATAAATCA TGGCAAGACA ACATTCAGAC AGTGCTGTTT 
ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAAGAAC ATGTTGAAAT GCTCCAGGAA 
ATAACTCCCA CCTTGAAAGA ACTGAAAACG CAAAGTCAGA GTGTGTATAA TAATTTAGTG 
AGTTTTGCAT CACCATTAGT GACCGATGCA ACAAATGAAT GTTCGAGTCC AACATCGTCT 
GCTACTTATC AACCATCCTT TGCTGCAGCA GTCCGAAGCA ACACTGGCCA GAAGACTCAG 
CCTGATGTCA TGTCACAGAA CGCTAGAAAG CTGATTCAGA AAAACCTAGC TACATCAGCA 
GATACCCCAC CGAGCACCGT TCCGGGAACT GGCAAGAGTG TTGCTTGTAG TCCTAAAAAG 
GCCATCAGAG ACCCTAAGAC TGGAAAAGCT GTGCAGGAGA GAAACTCGTA TGCGGTGAGT 
GTGTGGAAGA GAGTGAAAGC CAAGCTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG 
TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT 
CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224802.1
CDS	25..9117
Translation	MWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRIGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNPSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSVVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANTGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFIECTEQLELLPGENISLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSITENVVKYLKQTSRIAVGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLATEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQSQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEHAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLENMLHELDSLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSIASCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005224745.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224745.3

ORF Insert Sequence:

ATGTGGGCCC TGTCTCCAAC TGTGTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAT 
GGCGACCTGG CTGTTCATTT CCCTGCCATT CAGTATGCAG TGCTCTACAC ATTGTATTCT 
CATTGTACCA GGCATGACCA CTTTATATCT AGTAGTCTTA GTTCATCATC CCCGTCCTTG 
TTTGATGGTG CCGTTATTAG TACTGTAACT ACAGCTACAA AAAAACATTT CTCAATTATC 
TTAAATCTCC TGGGAATATT GCTTAAGAAA GATAATCTTA ACCAGGACAC AAGGAAACTG 
TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATATGCACCT 
TTATTCTCTC TTCCATCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTGGTC 
GAAGATGTGA ATATCTGTCT GCAAGCATGC AGCAGTCTAC ATGCACTGTC CTCTTCCTTA 
CCAGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAGCTAGT CCATAGTGGA 
ACTCGTATTC GACAAGCATT TGGAAAGCTA TTGAAGTCAA TCCCTTTAGA TGTTGTCTTG 
AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT 
AAAGCACCAA GTAACACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG 
TATGGAAACT CTCATAGAAT AGGGAAGGAC AATTGGTTAG AAAGATTGTT CTATAGCTGC 
CAGAGACTGG ATAAACGTGA CCAATCAACG ATTCCCCGCA ATCTGCTAAA GACAGATGCT 
GTCCTCTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGCTGAGG 
ACCCCACTTG GCAGAGCTCA GGATACCTTC CAAACAATTG AAGGTATCAT TAGAAGTCTT 
GCAGCTCACA CATTAAACCC CGATCAAGAT GTTAGTCAGT GGACAACTGC AGACAATGAC 
GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTGCTTC TGCAGTATCT GGAAAACCTG 
GAGAAGTTAA TGTATAATGC ATATGAAGGA TGTGCCAATG CATTAACATC GCCTCCCAAG 
GTCATTAGGA CTTTTTTCTA TACCAATCGC CAAACTTGTC AAGACTGGCT GACTCGGATC 
CGACTCTCCA TCATGAGGGT TGGATTGTTG GCAGGCCAGC CTGCTGTGAC AGTGAGACAC 
GGCTTCGATT TGCTTACAGA AATGAAGACA AATCCGTCTC AGGGAAATGA ATTGGAAGTA 
ACTATCATGA TGGTGGTAGA AGCATTGTGT GAACTTCATT GTCCTGAAGC TATACAGGGA 
ATTGCTGTCT GGTCGTCTTC GGTTGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC 
CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCTGTGGAGT ACCAGGAGCA CCTGTGTGCC 
ATGACAGGTG TCGATTGCTG CATCTCTAGC TTCGACAAAT CCGTTCTCAC ATTGGCTAAT 
ACTGGGCGTA ATAGTGCCAG CCCCAAGCAT TCTTTGAATG GTGAATCCAG AAAAACTGTG 
CTTTCCAAAC CAACTGATTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT 
GAGTGCTACA TTTCAATAGC TGATTGGGCT GCTGTACAGG AGTGGCAGAA TGCTGTCCAT 
GACTTGAAAA AGAGTACCAG TAGTACTTCT CTCAACCTAA AGGCTGATTT CAACTACATA 
AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTATTGAAT GCACTGAACA ATTAGAATTG 
TTACCAGGAG AAAATATCAG TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA 
AAACTGCTTC CTAACATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATTGAAGTT 
CAGTTGTTAA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCACATAGA ACAAGATCAG 
AAGTGGCAGT CTATTACCGA AAATGTGGTG AAGTACCTGA AGCAAACCTC CCGCATAGCT 
GTTGGACCCC TGAGACTTTC TACTCTAACA GTTTCCCAGT CTCTGCCAGT CCTAAGTACT 
TTACAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TGCCACAGAG 
GACTGTCTTA TTCCACTCTT TAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG 
CCATGGATGC AAGCATTAAG ATACACGATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA 
GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT 
GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGATTGC TGGCACAGTG TAGCGAGGTT 
CAGCTGGGAA AGACTACCAC TGCACAGGAT TTAGTCCAGC ACTTTAAAAA ACTATCAACT 
CAAGGTCAAG TGGATGAAAA ATGGGGGCCT GAACTTGACA TTGAAAAAAC GAAACTGCTA 
TATACAGCAG GCCAGTCAAC ACATGCAATG GAGATGTTGA GTTCCTGTGC CATCTCTTTC 
TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCAA TTTTGACATT GGCTAAATGG 
ATCCAGGCAG AATGGAAAGA AATCTCAGGA CAGCTGAAAC AGGTTTACAG AGCTCAGCAG 
CAGCAGAACT TCACGGGTCT TTCTACTTTG TCTAAAAACA TACTCACTCT AATTGAACTA 
CCATCTGTTA ATACGATGGA GGAAGAGTAT CCTCGGATAG AGAGTGAATC TACAGTACAT 
ATTGGAGTTG GGGAACCTGA CTTCATCTTG GGACAGCTGT ATCATCTGTC TTCAGTACAG 
GCACCTGAAG TAGCCAAATC TTGGGCCGCA TTGGCTAGTT GGGCTTACAG GTGGGGCAGA 
AAGGTGGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCTAG AGAAAAATCT 
GAAGTTCAGA ATCTGCTCCC AGACACCATA ACCGAAGAAG AAAAAGAGAG AATATATGGT 
ATTCTTGGTC AGGCTGTGTG TCGCCCAGCG GGGATTCAGG ATGAAGATAT AACGCTTCAG 
ATCACAGAAA GTGAAGATAA TGAGGAAGAT GACATGGTTG ATGTTATCTG GCGTCAGTTA 
ATATCAAGTT GCCCATGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG AGTCATTAAA 
GTGTGGAGGA AAGTTGTGGA CAGAATCTTC AGCCTGTATA AACTGTCCTG CAGCGCATAT 
TTTACTTTCC TTAAACTCAA TGCTGGGCAG ATTCCTTTAG ATGAAGATGA CCCTCGGCTC 
CATTTAAGCC ACAGAGTGGA GCAGAGCACG GATGACATGA TTGTGATGGC CACGCTGCGG 
TTGCTCAGGT TGCTCGTGAA GCACGCTGGG GAGCTTCGGC AGTATCTGGA ACATGGCTTG 
GAAACAACAC CTACTGCGCC TTGGAGAGGA ATTATTCCGC AGCTTTTCTC ACGCTTAAAC 
CACCCTGAAG TGTATGTGCG CCAGAGTATT TGTAACCTCC TCTGCCGCGT GGCTCAGGAT 
TCCCCGCATC TCATACTGTA TCCTGCAATA GTGGGCACCA TATCGCTTAG TAGTGAGTCA 
CAGGCTTCAG GAAATAAATT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA 
GAAGAACTAC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCTCA AGATAGCAAT 
AAGGATGAAC CTAAAAGTGG ACTGAATGAA GACCAAGCCA TGATGCAGGA CTGCTACAGC 
AAAATTGTAG ATAAACTGTC CTCGGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTT 
GTGGCTGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA 
CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGTTGG AAGACGAGGT GAAGAGAGTC 
CAGAACAACA ACACCTTACG CAAAGAAGAG AAAATCGCAA TCATGCGGGA GAAGCACACA 
GCTTTGATGA AGCCCATCGT ATTTGCTTTG GAGCACGTTA GGAGCATCAC AGCGGCCCCT 
GCAGAAACAC CTCATGAAAA GTGGTTTCAG GATAACTATG GTGATGCCAT TGAAAATGCT 
CTAGAAAAGC TGAAAACACC GTCAAACCCT GCAAAGCCTG GAAGCAGCTG GATTCCATTT 
AAAGAGATAA TGCTGAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGTTA CATCTTGCGT 
CTTGAAGAAA TCAGCCCATG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCCGGA 
GAAGTCTCAG CCAGAGATAC CGTCACAATC CACAGTGTTG GTGGAACCAT CACAATCTTA 
CCTACTAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CAGATGGGAA GAGCTATCCT 
TATCTTTTCA AAGGATTGGA GGATTTACAT CTGGATGAGA GAATAATGCA ATTTCTGTCT 
ATTGTAAATA CCATGTTTGC AACAATCAAT CGCCAGGAAA CACCTCGTTT CCATGCTCGC 
CACTATTCTG TAACACCACT GGGAACTCGC TCAGGCCTAA TCCAGTGGGT GGATGGAGCC 
ACGCCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCCGCCTT GCAAGCACAA 
AAGGCCCAAG ATTCCTACCA AACTCCTCAG AATCCTGGAA TCGTACCCCG TCCTAGTGAA 
CTTTATTATA GTAAAATTGG TCCTGCTTTG AAAGCAGTTG GGCTTAGTCT GGATGTGTCC 
CGCCGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGTTAG AAGAGTTAAT GGAGGCCACG 
CCCCCAAATC TCCTTGCTAA AGAGCTTTGG TCATCATGTA CCACCCCTGA TGAGTGGTGG 
AGAGTCACAC AGTCTTATGC CAGATCTACT GCAGTCATGT CTATGGTCGG ATACATAATT 
GGCCTTGGAG ATAGACATCT GGATAATGTT CTCATAGATA TGACGACTGG AGAGGTTGTT 
CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTA 
CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT 
TTTAGGCTTT CATGTGAGCA GGTTCTACAC ATTATGCGGC GTGGCAGAGA GACTCTGCTG 
ACGCTGCTGG AGGCCTTTGT ATATGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT 
GGGTTTGCTG GTGCGGTCTA TGGTGGAGGG GGCCAGCAGG CTGAGAGCAA GCAGAGCAAG 
AGAGAGATGG AACGAGAGAT TACTCGCAGC CTTTTTTCTT CGAGAGTAGC TGAAATTAAG 
GTGAACTGGT TTAAAAACAG AGATGAGATG CTGGTTGTAC TTCCCAAGCT GGACAGCAGC 
TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTAACAGATG TGGAGAAACT GCAGGGCAAA 
CTGCTGGAAG AAATTGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT 
CTGCAACACA GGTATTCTGA ACACACTCAA CTACAGACTC AGCAGAGAGC TGTTCAGGAA 
GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACACTATCA GGCTGCGTTC 
AATAATTTGG AAGCTACACA GCTGGCCAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC 
CTTGGTCCTC CCAGCTACGT GCCGGCAACA GCCTTTCTGC AGAATGCTGG CCAGGCCCAC 
TTGATTAGCC AGTGTGAGCA GCTAGAAGGG GAGGTTGGTG CCCTCCTGCA GCAGAGGCGC 
TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTACG CAACTGTTGC TCTGCAGTAT 
CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAGCTC 
ATCTGTAATA CCACAGTAGA ACGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT 
GCGCCCCAGC CACCCCCAAC AGTGTGTCAG TTCATCACGG CCACTGAGAT GACCCTGCAG 
CGGTACGCAG CCGACATCAA CAGCCGACTT ATCAGACAGG TGGAACGCTT GAAACAGGAA 
GCTGTCACTG TACCGGTTTG TGAAGATCAG TTGAAAGAAA TTGAACGTTG TATCAAAGTT 
TTCCTTCACG AGAATGGAGA GGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATTTCTGCC 
CTTTGTACTC TCACAAGGCG TAACCTAATG ATGGAAGGTG CAGCGTCGAG TGCTGGAGAG 
CAGCTGGTTG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAACT CTGCAGTATG 
AGCGGAAATG TCACATGTCT GGTTCAGTTA CTGAAGCAGT GTCATTTAGT GCCACAAGAC 
TTAGACATTC CTAACCCCAT GGAAGCATCT GAGGCCGTTC ATTTGGCTAA TGGTGTATAC 
ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA 
TGTTTAATGA AAGGAGAATA CACGTTAGAA AATATGCTTC ATGAGCTGGA CAGTCTTATT 
GAGCAGACAA CTGATGGTGT TCCTCTGCAG ACTCTAGTTG AATCTCTTCA AGCCTATTTA 
AGAAATGCAG CTATGGGACT TGAGGAAGAA ACACATGCTC ATTACATCGA TGTTGCCAGA 
ATGCTTCATG CTCAGTATGG TGAATTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA 
CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCATTTG ATGGCATGTT TGCCCAAGTT 
GAGACTGCTT TTGGCTTATT GGTTGAAAAG CTAAATAAGA TGGAAATTCC TATAGCTTGG 
CGAAAGATTG ACATAATACG GGAAGCCAGG AGCACTCAGG TCAATTTTTT TGATGATGAT 
AATCACCGAC AGGTGCTGGA AGAGATCTTC TTTCTAAAAC GACTACAGAC AATTAAGGAG 
TTCTTTCGGC TCTGTGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT 
CAGAATACTG TAAATGGGCC TGTACAGATT GTCAATGTGA AAACCCTTTT TAGAAACTCT 
TGTTTCAGTG AAGACCAGAT GGCCAAACCA ATAAAGGCAT TCACAGCTGA CTTTGTGCGG 
CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CACTGTGCAG CTTCATCAGT 
GCTCTAGGGG TAGACATCAT TGCGCAGGTG GAGGCGAAGG ATTTTGGTGC TGAAAGCAAA 
GTTTCTGTTG ACGATCTGTG TAAGAAGGCG GTGGAGCACA ACATCCAGAT CGGGAAGTTC 
TCTCAGCTGG TCATGAACAG GGCGACTGTG CTGGCGAGCT CCTACGACAC TGCCTGGAAG 
AAGCACGACT TGGTGCGCAG GCTGGAGACC AGCATCGCCT CCTGCAAGAC AAGCCTGCAG 
CGTGTCCAGC TGCACATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAATAGA 
CCACAAGCCA TGTCTGTCAC ACCTCCCCCT CGTTCTGCCA TATTAACCAG CATGAAAAAG 
AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA 
GCACTTGAAG CAAGTATTGA ACAGCGACTC AAATGGGCAG GTGGTGCCAA CCCTGCACTG 
GCACCTGTAC TACAGGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAG 
GAGAGCCAAA GAGCAAGTCA GGTCACTTTC CTCTGCAGCA ATATCATTCA TTTTGAAAGT 
TTACGAACTA GAACTGCAGA AGCCTTAAAC CTGGATGCTG CATTATTTGA ACTAATCAAA 
CGGTGTCAGC AAATGTGTTC ATTTGCATCG CAGTTTAACA GTTCAGTCTC TGAGTTAGAG 
CTTCGTTTAT TACAGAGGGT GGACACGACT CTTGAACATC CTATTGGCAG CTCTGAGTGG 
CTTTTGTCAG CACACAAACA ACTGACCCAG GATATGTCTA CTCAGAGAGC AATTCAGACA 
GAGAAAGAGC AACAAATAGA AACAGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA 
AAGACTGTGC TCACTGGCCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA 
ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGTGAAG ATGTTCCCTA TGAGAACAGT 
GTTAGGCAGT TCTTGGGTGA ATATAAATCA TGGCAAGACA ACATTCAGAC AGTGCTGTTT 
ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAAGAAC ATGTTGAAAT GCTCCAGGAA 
ATAACTCCCA CCTTGAAAGA ACTGAAAACG CAAAGTCAGA GTGTGTATAA TAATTTAGTG 
AGTTTTGCAT CACCATTAGT GACCGATGCA ACAAATGAAT GTTCGAGTCC AACATCGTCT 
GCTACTTATC AACCATCCTT TGCTGCAGCA GTCCGAAGCA ACACTGGCCA GAAGACTCAG 
CCTGATGTCA TGTCACAGAA CGCTAGAAAG CTGATTCAGA AAAACCTAGC TACATCAGCA 
GATACCCCAC CGAGCACCGT TCCGGGAACT GGCAAGAGTG TTGCTTGTAG TCCTAAAAAG 
GCCATCAGAG ACCCTAAGAC TGGAAAAGCT GTGCAGGAGA GAAACTCGTA TGCGGTGAGT 
GTGTGGAAGA GAGTGAAAGC CAAGCTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG 
TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT 
CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005224745.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224802.1 Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.

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CloneID	OBa32979
Clone ID Related Accession (Same CDS sequence)	XM_005224745.3 , XM_005224745.4
Accession Version	XM_005224745.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase SMG1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224745.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCC TGTCTCCAAC TGTGTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAT 
GGCGACCTGG CTGTTCATTT CCCTGCCATT CAGTATGCAG TGCTCTACAC ATTGTATTCT 
CATTGTACCA GGCATGACCA CTTTATATCT AGTAGTCTTA GTTCATCATC CCCGTCCTTG 
TTTGATGGTG CCGTTATTAG TACTGTAACT ACAGCTACAA AAAAACATTT CTCAATTATC 
TTAAATCTCC TGGGAATATT GCTTAAGAAA GATAATCTTA ACCAGGACAC AAGGAAACTG 
TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATATGCACCT 
TTATTCTCTC TTCCATCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTGGTC 
GAAGATGTGA ATATCTGTCT GCAAGCATGC AGCAGTCTAC ATGCACTGTC CTCTTCCTTA 
CCAGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAGCTAGT CCATAGTGGA 
ACTCGTATTC GACAAGCATT TGGAAAGCTA TTGAAGTCAA TCCCTTTAGA TGTTGTCTTG 
AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT 
AAAGCACCAA GTAACACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG 
TATGGAAACT CTCATAGAAT AGGGAAGGAC AATTGGTTAG AAAGATTGTT CTATAGCTGC 
CAGAGACTGG ATAAACGTGA CCAATCAACG ATTCCCCGCA ATCTGCTAAA GACAGATGCT 
GTCCTCTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGCTGAGG 
ACCCCACTTG GCAGAGCTCA GGATACCTTC CAAACAATTG AAGGTATCAT TAGAAGTCTT 
GCAGCTCACA CATTAAACCC CGATCAAGAT GTTAGTCAGT GGACAACTGC AGACAATGAC 
GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTGCTTC TGCAGTATCT GGAAAACCTG 
GAGAAGTTAA TGTATAATGC ATATGAAGGA TGTGCCAATG CATTAACATC GCCTCCCAAG 
GTCATTAGGA CTTTTTTCTA TACCAATCGC CAAACTTGTC AAGACTGGCT GACTCGGATC 
CGACTCTCCA TCATGAGGGT TGGATTGTTG GCAGGCCAGC CTGCTGTGAC AGTGAGACAC 
GGCTTCGATT TGCTTACAGA AATGAAGACA AATCCGTCTC AGGGAAATGA ATTGGAAGTA 
ACTATCATGA TGGTGGTAGA AGCATTGTGT GAACTTCATT GTCCTGAAGC TATACAGGGA 
ATTGCTGTCT GGTCGTCTTC GGTTGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC 
CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCTGTGGAGT ACCAGGAGCA CCTGTGTGCC 
ATGACAGGTG TCGATTGCTG CATCTCTAGC TTCGACAAAT CCGTTCTCAC ATTGGCTAAT 
ACTGGGCGTA ATAGTGCCAG CCCCAAGCAT TCTTTGAATG GTGAATCCAG AAAAACTGTG 
CTTTCCAAAC CAACTGATTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT 
GAGTGCTACA TTTCAATAGC TGATTGGGCT GCTGTACAGG AGTGGCAGAA TGCTGTCCAT 
GACTTGAAAA AGAGTACCAG TAGTACTTCT CTCAACCTAA AGGCTGATTT CAACTACATA 
AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTATTGAAT GCACTGAACA ATTAGAATTG 
TTACCAGGAG AAAATATCAG TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA 
AAACTGCTTC CTAACATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATTGAAGTT 
CAGTTGTTAA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCACATAGA ACAAGATCAG 
AAGTGGCAGT CTATTACCGA AAATGTGGTG AAGTACCTGA AGCAAACCTC CCGCATAGCT 
GTTGGACCCC TGAGACTTTC TACTCTAACA GTTTCCCAGT CTCTGCCAGT CCTAAGTACT 
TTACAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TGCCACAGAG 
GACTGTCTTA TTCCACTCTT TAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG 
CCATGGATGC AAGCATTAAG ATACACGATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA 
GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT 
GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGATTGC TGGCACAGTG TAGCGAGGTT 
CAGCTGGGAA AGACTACCAC TGCACAGGAT TTAGTCCAGC ACTTTAAAAA ACTATCAACT 
CAAGGTCAAG TGGATGAAAA ATGGGGGCCT GAACTTGACA TTGAAAAAAC GAAACTGCTA 
TATACAGCAG GCCAGTCAAC ACATGCAATG GAGATGTTGA GTTCCTGTGC CATCTCTTTC 
TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCAA TTTTGACATT GGCTAAATGG 
ATCCAGGCAG AATGGAAAGA AATCTCAGGA CAGCTGAAAC AGGTTTACAG AGCTCAGCAG 
CAGCAGAACT TCACGGGTCT TTCTACTTTG TCTAAAAACA TACTCACTCT AATTGAACTA 
CCATCTGTTA ATACGATGGA GGAAGAGTAT CCTCGGATAG AGAGTGAATC TACAGTACAT 
ATTGGAGTTG GGGAACCTGA CTTCATCTTG GGACAGCTGT ATCATCTGTC TTCAGTACAG 
GCACCTGAAG TAGCCAAATC TTGGGCCGCA TTGGCTAGTT GGGCTTACAG GTGGGGCAGA 
AAGGTGGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCTAG AGAAAAATCT 
GAAGTTCAGA ATCTGCTCCC AGACACCATA ACCGAAGAAG AAAAAGAGAG AATATATGGT 
ATTCTTGGTC AGGCTGTGTG TCGCCCAGCG GGGATTCAGG ATGAAGATAT AACGCTTCAG 
ATCACAGAAA GTGAAGATAA TGAGGAAGAT GACATGGTTG ATGTTATCTG GCGTCAGTTA 
ATATCAAGTT GCCCATGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG AGTCATTAAA 
GTGTGGAGGA AAGTTGTGGA CAGAATCTTC AGCCTGTATA AACTGTCCTG CAGCGCATAT 
TTTACTTTCC TTAAACTCAA TGCTGGGCAG ATTCCTTTAG ATGAAGATGA CCCTCGGCTC 
CATTTAAGCC ACAGAGTGGA GCAGAGCACG GATGACATGA TTGTGATGGC CACGCTGCGG 
TTGCTCAGGT TGCTCGTGAA GCACGCTGGG GAGCTTCGGC AGTATCTGGA ACATGGCTTG 
GAAACAACAC CTACTGCGCC TTGGAGAGGA ATTATTCCGC AGCTTTTCTC ACGCTTAAAC 
CACCCTGAAG TGTATGTGCG CCAGAGTATT TGTAACCTCC TCTGCCGCGT GGCTCAGGAT 
TCCCCGCATC TCATACTGTA TCCTGCAATA GTGGGCACCA TATCGCTTAG TAGTGAGTCA 
CAGGCTTCAG GAAATAAATT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA 
GAAGAACTAC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCTCA AGATAGCAAT 
AAGGATGAAC CTAAAAGTGG ACTGAATGAA GACCAAGCCA TGATGCAGGA CTGCTACAGC 
AAAATTGTAG ATAAACTGTC CTCGGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTT 
GTGGCTGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA 
CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGTTGG AAGACGAGGT GAAGAGAGTC 
CAGAACAACA ACACCTTACG CAAAGAAGAG AAAATCGCAA TCATGCGGGA GAAGCACACA 
GCTTTGATGA AGCCCATCGT ATTTGCTTTG GAGCACGTTA GGAGCATCAC AGCGGCCCCT 
GCAGAAACAC CTCATGAAAA GTGGTTTCAG GATAACTATG GTGATGCCAT TGAAAATGCT 
CTAGAAAAGC TGAAAACACC GTCAAACCCT GCAAAGCCTG GAAGCAGCTG GATTCCATTT 
AAAGAGATAA TGCTGAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGTTA CATCTTGCGT 
CTTGAAGAAA TCAGCCCATG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCCGGA 
GAAGTCTCAG CCAGAGATAC CGTCACAATC CACAGTGTTG GTGGAACCAT CACAATCTTA 
CCTACTAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CAGATGGGAA GAGCTATCCT 
TATCTTTTCA AAGGATTGGA GGATTTACAT CTGGATGAGA GAATAATGCA ATTTCTGTCT 
ATTGTAAATA CCATGTTTGC AACAATCAAT CGCCAGGAAA CACCTCGTTT CCATGCTCGC 
CACTATTCTG TAACACCACT GGGAACTCGC TCAGGCCTAA TCCAGTGGGT GGATGGAGCC 
ACGCCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCCGCCTT GCAAGCACAA 
AAGGCCCAAG ATTCCTACCA AACTCCTCAG AATCCTGGAA TCGTACCCCG TCCTAGTGAA 
CTTTATTATA GTAAAATTGG TCCTGCTTTG AAAGCAGTTG GGCTTAGTCT GGATGTGTCC 
CGCCGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGTTAG AAGAGTTAAT GGAGGCCACG 
CCCCCAAATC TCCTTGCTAA AGAGCTTTGG TCATCATGTA CCACCCCTGA TGAGTGGTGG 
AGAGTCACAC AGTCTTATGC CAGATCTACT GCAGTCATGT CTATGGTCGG ATACATAATT 
GGCCTTGGAG ATAGACATCT GGATAATGTT CTCATAGATA TGACGACTGG AGAGGTTGTT 
CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTA 
CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT 
TTTAGGCTTT CATGTGAGCA GGTTCTACAC ATTATGCGGC GTGGCAGAGA GACTCTGCTG 
ACGCTGCTGG AGGCCTTTGT ATATGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT 
GGGTTTGCTG GTGCGGTCTA TGGTGGAGGG GGCCAGCAGG CTGAGAGCAA GCAGAGCAAG 
AGAGAGATGG AACGAGAGAT TACTCGCAGC CTTTTTTCTT CGAGAGTAGC TGAAATTAAG 
GTGAACTGGT TTAAAAACAG AGATGAGATG CTGGTTGTAC TTCCCAAGCT GGACAGCAGC 
TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTAACAGATG TGGAGAAACT GCAGGGCAAA 
CTGCTGGAAG AAATTGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT 
CTGCAACACA GGTATTCTGA ACACACTCAA CTACAGACTC AGCAGAGAGC TGTTCAGGAA 
GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACACTATCA GGCTGCGTTC 
AATAATTTGG AAGCTACACA GCTGGCCAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC 
CTTGGTCCTC CCAGCTACGT GCCGGCAACA GCCTTTCTGC AGAATGCTGG CCAGGCCCAC 
TTGATTAGCC AGTGTGAGCA GCTAGAAGGG GAGGTTGGTG CCCTCCTGCA GCAGAGGCGC 
TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTACG CAACTGTTGC TCTGCAGTAT 
CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAGCTC 
ATCTGTAATA CCACAGTAGA ACGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT 
GCGCCCCAGC CACCCCCAAC AGTGTGTCAG TTCATCACGG CCACTGAGAT GACCCTGCAG 
CGGTACGCAG CCGACATCAA CAGCCGACTT ATCAGACAGG TGGAACGCTT GAAACAGGAA 
GCTGTCACTG TACCGGTTTG TGAAGATCAG TTGAAAGAAA TTGAACGTTG TATCAAAGTT 
TTCCTTCACG AGAATGGAGA GGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATTTCTGCC 
CTTTGTACTC TCACAAGGCG TAACCTAATG ATGGAAGGTG CAGCGTCGAG TGCTGGAGAG 
CAGCTGGTTG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAACT CTGCAGTATG 
AGCGGAAATG TCACATGTCT GGTTCAGTTA CTGAAGCAGT GTCATTTAGT GCCACAAGAC 
TTAGACATTC CTAACCCCAT GGAAGCATCT GAGGCCGTTC ATTTGGCTAA TGGTGTATAC 
ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA 
TGTTTAATGA AAGGAGAATA CACGTTAGAA AATATGCTTC ATGAGCTGGA CAGTCTTATT 
GAGCAGACAA CTGATGGTGT TCCTCTGCAG ACTCTAGTTG AATCTCTTCA AGCCTATTTA 
AGAAATGCAG CTATGGGACT TGAGGAAGAA ACACATGCTC ATTACATCGA TGTTGCCAGA 
ATGCTTCATG CTCAGTATGG TGAATTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA 
CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCATTTG ATGGCATGTT TGCCCAAGTT 
GAGACTGCTT TTGGCTTATT GGTTGAAAAG CTAAATAAGA TGGAAATTCC TATAGCTTGG 
CGAAAGATTG ACATAATACG GGAAGCCAGG AGCACTCAGG TCAATTTTTT TGATGATGAT 
AATCACCGAC AGGTGCTGGA AGAGATCTTC TTTCTAAAAC GACTACAGAC AATTAAGGAG 
TTCTTTCGGC TCTGTGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT 
CAGAATACTG TAAATGGGCC TGTACAGATT GTCAATGTGA AAACCCTTTT TAGAAACTCT 
TGTTTCAGTG AAGACCAGAT GGCCAAACCA ATAAAGGCAT TCACAGCTGA CTTTGTGCGG 
CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CACTGTGCAG CTTCATCAGT 
GCTCTAGGGG TAGACATCAT TGCGCAGGTG GAGGCGAAGG ATTTTGGTGC TGAAAGCAAA 
GTTTCTGTTG ACGATCTGTG TAAGAAGGCG GTGGAGCACA ACATCCAGAT CGGGAAGTTC 
TCTCAGCTGG TCATGAACAG GGCGACTGTG CTGGCGAGCT CCTACGACAC TGCCTGGAAG 
AAGCACGACT TGGTGCGCAG GCTGGAGACC AGCATCGCCT CCTGCAAGAC AAGCCTGCAG 
CGTGTCCAGC TGCACATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAATAGA 
CCACAAGCCA TGTCTGTCAC ACCTCCCCCT CGTTCTGCCA TATTAACCAG CATGAAAAAG 
AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA 
GCACTTGAAG CAAGTATTGA ACAGCGACTC AAATGGGCAG GTGGTGCCAA CCCTGCACTG 
GCACCTGTAC TACAGGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAG 
GAGAGCCAAA GAGCAAGTCA GGTCACTTTC CTCTGCAGCA ATATCATTCA TTTTGAAAGT 
TTACGAACTA GAACTGCAGA AGCCTTAAAC CTGGATGCTG CATTATTTGA ACTAATCAAA 
CGGTGTCAGC AAATGTGTTC ATTTGCATCG CAGTTTAACA GTTCAGTCTC TGAGTTAGAG 
CTTCGTTTAT TACAGAGGGT GGACACGACT CTTGAACATC CTATTGGCAG CTCTGAGTGG 
CTTTTGTCAG CACACAAACA ACTGACCCAG GATATGTCTA CTCAGAGAGC AATTCAGACA 
GAGAAAGAGC AACAAATAGA AACAGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA 
AAGACTGTGC TCACTGGCCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA 
ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGTGAAG ATGTTCCCTA TGAGAACAGT 
GTTAGGCAGT TCTTGGGTGA ATATAAATCA TGGCAAGACA ACATTCAGAC AGTGCTGTTT 
ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAAGAAC ATGTTGAAAT GCTCCAGGAA 
ATAACTCCCA CCTTGAAAGA ACTGAAAACG CAAAGTCAGA GTGTGTATAA TAATTTAGTG 
AGTTTTGCAT CACCATTAGT GACCGATGCA ACAAATGAAT GTTCGAGTCC AACATCGTCT 
GCTACTTATC AACCATCCTT TGCTGCAGCA GTCCGAAGCA ACACTGGCCA GAAGACTCAG 
CCTGATGTCA TGTCACAGAA CGCTAGAAAG CTGATTCAGA AAAACCTAGC TACATCAGCA 
GATACCCCAC CGAGCACCGT TCCGGGAACT GGCAAGAGTG TTGCTTGTAG TCCTAAAAAG 
GCCATCAGAG ACCCTAAGAC TGGAAAAGCT GTGCAGGAGA GAAACTCGTA TGCGGTGAGT 
GTGTGGAAGA GAGTGAAAGC CAAGCTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG 
TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT 
CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224802.1
CDS	39..9131
Translation	MWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRIGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNPSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSVVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANTGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFIECTEQLELLPGENISLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSITENVVKYLKQTSRIAVGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLATEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQSQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEHAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLENMLHELDSLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSIASCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005224745.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224745.4

ORF Insert Sequence:

ATGTGGGCCC TGTCTCCAAC TGTGTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAT 
GGCGACCTGG CTGTTCATTT CCCTGCCATT CAGTATGCAG TGCTCTACAC ATTGTATTCT 
CATTGTACCA GGCATGACCA CTTTATATCT AGTAGTCTTA GTTCATCATC CCCGTCCTTG 
TTTGATGGTG CCGTTATTAG TACTGTAACT ACAGCTACAA AAAAACATTT CTCAATTATC 
TTAAATCTCC TGGGAATATT GCTTAAGAAA GATAATCTTA ACCAGGACAC AAGGAAACTG 
TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATATGCACCT 
TTATTCTCTC TTCCATCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTGGTC 
GAAGATGTGA ATATCTGTCT GCAAGCATGC AGCAGTCTAC ATGCACTGTC CTCTTCCTTA 
CCAGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAGCTAGT CCATAGTGGA 
ACTCGTATTC GACAAGCATT TGGAAAGCTA TTGAAGTCAA TCCCTTTAGA TGTTGTCTTG 
AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT 
AAAGCACCAA GTAACACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG 
TATGGAAACT CTCATAGAAT AGGGAAGGAC AATTGGTTAG AAAGATTGTT CTATAGCTGC 
CAGAGACTGG ATAAACGTGA CCAATCAACG ATTCCCCGCA ATCTGCTAAA GACAGATGCT 
GTCCTCTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGCTGAGG 
ACCCCACTTG GCAGAGCTCA GGATACCTTC CAAACAATTG AAGGTATCAT TAGAAGTCTT 
GCAGCTCACA CATTAAACCC CGATCAAGAT GTTAGTCAGT GGACAACTGC AGACAATGAC 
GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTGCTTC TGCAGTATCT GGAAAACCTG 
GAGAAGTTAA TGTATAATGC ATATGAAGGA TGTGCCAATG CATTAACATC GCCTCCCAAG 
GTCATTAGGA CTTTTTTCTA TACCAATCGC CAAACTTGTC AAGACTGGCT GACTCGGATC 
CGACTCTCCA TCATGAGGGT TGGATTGTTG GCAGGCCAGC CTGCTGTGAC AGTGAGACAC 
GGCTTCGATT TGCTTACAGA AATGAAGACA AATCCGTCTC AGGGAAATGA ATTGGAAGTA 
ACTATCATGA TGGTGGTAGA AGCATTGTGT GAACTTCATT GTCCTGAAGC TATACAGGGA 
ATTGCTGTCT GGTCGTCTTC GGTTGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC 
CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCTGTGGAGT ACCAGGAGCA CCTGTGTGCC 
ATGACAGGTG TCGATTGCTG CATCTCTAGC TTCGACAAAT CCGTTCTCAC ATTGGCTAAT 
ACTGGGCGTA ATAGTGCCAG CCCCAAGCAT TCTTTGAATG GTGAATCCAG AAAAACTGTG 
CTTTCCAAAC CAACTGATTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT 
GAGTGCTACA TTTCAATAGC TGATTGGGCT GCTGTACAGG AGTGGCAGAA TGCTGTCCAT 
GACTTGAAAA AGAGTACCAG TAGTACTTCT CTCAACCTAA AGGCTGATTT CAACTACATA 
AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTATTGAAT GCACTGAACA ATTAGAATTG 
TTACCAGGAG AAAATATCAG TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA 
AAACTGCTTC CTAACATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATTGAAGTT 
CAGTTGTTAA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCACATAGA ACAAGATCAG 
AAGTGGCAGT CTATTACCGA AAATGTGGTG AAGTACCTGA AGCAAACCTC CCGCATAGCT 
GTTGGACCCC TGAGACTTTC TACTCTAACA GTTTCCCAGT CTCTGCCAGT CCTAAGTACT 
TTACAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TGCCACAGAG 
GACTGTCTTA TTCCACTCTT TAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG 
CCATGGATGC AAGCATTAAG ATACACGATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA 
GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT 
GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGATTGC TGGCACAGTG TAGCGAGGTT 
CAGCTGGGAA AGACTACCAC TGCACAGGAT TTAGTCCAGC ACTTTAAAAA ACTATCAACT 
CAAGGTCAAG TGGATGAAAA ATGGGGGCCT GAACTTGACA TTGAAAAAAC GAAACTGCTA 
TATACAGCAG GCCAGTCAAC ACATGCAATG GAGATGTTGA GTTCCTGTGC CATCTCTTTC 
TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCAA TTTTGACATT GGCTAAATGG 
ATCCAGGCAG AATGGAAAGA AATCTCAGGA CAGCTGAAAC AGGTTTACAG AGCTCAGCAG 
CAGCAGAACT TCACGGGTCT TTCTACTTTG TCTAAAAACA TACTCACTCT AATTGAACTA 
CCATCTGTTA ATACGATGGA GGAAGAGTAT CCTCGGATAG AGAGTGAATC TACAGTACAT 
ATTGGAGTTG GGGAACCTGA CTTCATCTTG GGACAGCTGT ATCATCTGTC TTCAGTACAG 
GCACCTGAAG TAGCCAAATC TTGGGCCGCA TTGGCTAGTT GGGCTTACAG GTGGGGCAGA 
AAGGTGGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCTAG AGAAAAATCT 
GAAGTTCAGA ATCTGCTCCC AGACACCATA ACCGAAGAAG AAAAAGAGAG AATATATGGT 
ATTCTTGGTC AGGCTGTGTG TCGCCCAGCG GGGATTCAGG ATGAAGATAT AACGCTTCAG 
ATCACAGAAA GTGAAGATAA TGAGGAAGAT GACATGGTTG ATGTTATCTG GCGTCAGTTA 
ATATCAAGTT GCCCATGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG AGTCATTAAA 
GTGTGGAGGA AAGTTGTGGA CAGAATCTTC AGCCTGTATA AACTGTCCTG CAGCGCATAT 
TTTACTTTCC TTAAACTCAA TGCTGGGCAG ATTCCTTTAG ATGAAGATGA CCCTCGGCTC 
CATTTAAGCC ACAGAGTGGA GCAGAGCACG GATGACATGA TTGTGATGGC CACGCTGCGG 
TTGCTCAGGT TGCTCGTGAA GCACGCTGGG GAGCTTCGGC AGTATCTGGA ACATGGCTTG 
GAAACAACAC CTACTGCGCC TTGGAGAGGA ATTATTCCGC AGCTTTTCTC ACGCTTAAAC 
CACCCTGAAG TGTATGTGCG CCAGAGTATT TGTAACCTCC TCTGCCGCGT GGCTCAGGAT 
TCCCCGCATC TCATACTGTA TCCTGCAATA GTGGGCACCA TATCGCTTAG TAGTGAGTCA 
CAGGCTTCAG GAAATAAATT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA 
GAAGAACTAC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCTCA AGATAGCAAT 
AAGGATGAAC CTAAAAGTGG ACTGAATGAA GACCAAGCCA TGATGCAGGA CTGCTACAGC 
AAAATTGTAG ATAAACTGTC CTCGGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTT 
GTGGCTGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA 
CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGTTGG AAGACGAGGT GAAGAGAGTC 
CAGAACAACA ACACCTTACG CAAAGAAGAG AAAATCGCAA TCATGCGGGA GAAGCACACA 
GCTTTGATGA AGCCCATCGT ATTTGCTTTG GAGCACGTTA GGAGCATCAC AGCGGCCCCT 
GCAGAAACAC CTCATGAAAA GTGGTTTCAG GATAACTATG GTGATGCCAT TGAAAATGCT 
CTAGAAAAGC TGAAAACACC GTCAAACCCT GCAAAGCCTG GAAGCAGCTG GATTCCATTT 
AAAGAGATAA TGCTGAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGTTA CATCTTGCGT 
CTTGAAGAAA TCAGCCCATG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCCGGA 
GAAGTCTCAG CCAGAGATAC CGTCACAATC CACAGTGTTG GTGGAACCAT CACAATCTTA 
CCTACTAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CAGATGGGAA GAGCTATCCT 
TATCTTTTCA AAGGATTGGA GGATTTACAT CTGGATGAGA GAATAATGCA ATTTCTGTCT 
ATTGTAAATA CCATGTTTGC AACAATCAAT CGCCAGGAAA CACCTCGTTT CCATGCTCGC 
CACTATTCTG TAACACCACT GGGAACTCGC TCAGGCCTAA TCCAGTGGGT GGATGGAGCC 
ACGCCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCCGCCTT GCAAGCACAA 
AAGGCCCAAG ATTCCTACCA AACTCCTCAG AATCCTGGAA TCGTACCCCG TCCTAGTGAA 
CTTTATTATA GTAAAATTGG TCCTGCTTTG AAAGCAGTTG GGCTTAGTCT GGATGTGTCC 
CGCCGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGTTAG AAGAGTTAAT GGAGGCCACG 
CCCCCAAATC TCCTTGCTAA AGAGCTTTGG TCATCATGTA CCACCCCTGA TGAGTGGTGG 
AGAGTCACAC AGTCTTATGC CAGATCTACT GCAGTCATGT CTATGGTCGG ATACATAATT 
GGCCTTGGAG ATAGACATCT GGATAATGTT CTCATAGATA TGACGACTGG AGAGGTTGTT 
CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTA 
CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT 
TTTAGGCTTT CATGTGAGCA GGTTCTACAC ATTATGCGGC GTGGCAGAGA GACTCTGCTG 
ACGCTGCTGG AGGCCTTTGT ATATGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT 
GGGTTTGCTG GTGCGGTCTA TGGTGGAGGG GGCCAGCAGG CTGAGAGCAA GCAGAGCAAG 
AGAGAGATGG AACGAGAGAT TACTCGCAGC CTTTTTTCTT CGAGAGTAGC TGAAATTAAG 
GTGAACTGGT TTAAAAACAG AGATGAGATG CTGGTTGTAC TTCCCAAGCT GGACAGCAGC 
TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTAACAGATG TGGAGAAACT GCAGGGCAAA 
CTGCTGGAAG AAATTGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT 
CTGCAACACA GGTATTCTGA ACACACTCAA CTACAGACTC AGCAGAGAGC TGTTCAGGAA 
GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACACTATCA GGCTGCGTTC 
AATAATTTGG AAGCTACACA GCTGGCCAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC 
CTTGGTCCTC CCAGCTACGT GCCGGCAACA GCCTTTCTGC AGAATGCTGG CCAGGCCCAC 
TTGATTAGCC AGTGTGAGCA GCTAGAAGGG GAGGTTGGTG CCCTCCTGCA GCAGAGGCGC 
TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTACG CAACTGTTGC TCTGCAGTAT 
CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAGCTC 
ATCTGTAATA CCACAGTAGA ACGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT 
GCGCCCCAGC CACCCCCAAC AGTGTGTCAG TTCATCACGG CCACTGAGAT GACCCTGCAG 
CGGTACGCAG CCGACATCAA CAGCCGACTT ATCAGACAGG TGGAACGCTT GAAACAGGAA 
GCTGTCACTG TACCGGTTTG TGAAGATCAG TTGAAAGAAA TTGAACGTTG TATCAAAGTT 
TTCCTTCACG AGAATGGAGA GGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATTTCTGCC 
CTTTGTACTC TCACAAGGCG TAACCTAATG ATGGAAGGTG CAGCGTCGAG TGCTGGAGAG 
CAGCTGGTTG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAACT CTGCAGTATG 
AGCGGAAATG TCACATGTCT GGTTCAGTTA CTGAAGCAGT GTCATTTAGT GCCACAAGAC 
TTAGACATTC CTAACCCCAT GGAAGCATCT GAGGCCGTTC ATTTGGCTAA TGGTGTATAC 
ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA 
TGTTTAATGA AAGGAGAATA CACGTTAGAA AATATGCTTC ATGAGCTGGA CAGTCTTATT 
GAGCAGACAA CTGATGGTGT TCCTCTGCAG ACTCTAGTTG AATCTCTTCA AGCCTATTTA 
AGAAATGCAG CTATGGGACT TGAGGAAGAA ACACATGCTC ATTACATCGA TGTTGCCAGA 
ATGCTTCATG CTCAGTATGG TGAATTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA 
CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCATTTG ATGGCATGTT TGCCCAAGTT 
GAGACTGCTT TTGGCTTATT GGTTGAAAAG CTAAATAAGA TGGAAATTCC TATAGCTTGG 
CGAAAGATTG ACATAATACG GGAAGCCAGG AGCACTCAGG TCAATTTTTT TGATGATGAT 
AATCACCGAC AGGTGCTGGA AGAGATCTTC TTTCTAAAAC GACTACAGAC AATTAAGGAG 
TTCTTTCGGC TCTGTGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT 
CAGAATACTG TAAATGGGCC TGTACAGATT GTCAATGTGA AAACCCTTTT TAGAAACTCT 
TGTTTCAGTG AAGACCAGAT GGCCAAACCA ATAAAGGCAT TCACAGCTGA CTTTGTGCGG 
CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CACTGTGCAG CTTCATCAGT 
GCTCTAGGGG TAGACATCAT TGCGCAGGTG GAGGCGAAGG ATTTTGGTGC TGAAAGCAAA 
GTTTCTGTTG ACGATCTGTG TAAGAAGGCG GTGGAGCACA ACATCCAGAT CGGGAAGTTC 
TCTCAGCTGG TCATGAACAG GGCGACTGTG CTGGCGAGCT CCTACGACAC TGCCTGGAAG 
AAGCACGACT TGGTGCGCAG GCTGGAGACC AGCATCGCCT CCTGCAAGAC AAGCCTGCAG 
CGTGTCCAGC TGCACATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAATAGA 
CCACAAGCCA TGTCTGTCAC ACCTCCCCCT CGTTCTGCCA TATTAACCAG CATGAAAAAG 
AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA 
GCACTTGAAG CAAGTATTGA ACAGCGACTC AAATGGGCAG GTGGTGCCAA CCCTGCACTG 
GCACCTGTAC TACAGGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAG 
GAGAGCCAAA GAGCAAGTCA GGTCACTTTC CTCTGCAGCA ATATCATTCA TTTTGAAAGT 
TTACGAACTA GAACTGCAGA AGCCTTAAAC CTGGATGCTG CATTATTTGA ACTAATCAAA 
CGGTGTCAGC AAATGTGTTC ATTTGCATCG CAGTTTAACA GTTCAGTCTC TGAGTTAGAG 
CTTCGTTTAT TACAGAGGGT GGACACGACT CTTGAACATC CTATTGGCAG CTCTGAGTGG 
CTTTTGTCAG CACACAAACA ACTGACCCAG GATATGTCTA CTCAGAGAGC AATTCAGACA 
GAGAAAGAGC AACAAATAGA AACAGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA 
AAGACTGTGC TCACTGGCCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA 
ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGTGAAG ATGTTCCCTA TGAGAACAGT 
GTTAGGCAGT TCTTGGGTGA ATATAAATCA TGGCAAGACA ACATTCAGAC AGTGCTGTTT 
ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAAGAAC ATGTTGAAAT GCTCCAGGAA 
ATAACTCCCA CCTTGAAAGA ACTGAAAACG CAAAGTCAGA GTGTGTATAA TAATTTAGTG 
AGTTTTGCAT CACCATTAGT GACCGATGCA ACAAATGAAT GTTCGAGTCC AACATCGTCT 
GCTACTTATC AACCATCCTT TGCTGCAGCA GTCCGAAGCA ACACTGGCCA GAAGACTCAG 
CCTGATGTCA TGTCACAGAA CGCTAGAAAG CTGATTCAGA AAAACCTAGC TACATCAGCA 
GATACCCCAC CGAGCACCGT TCCGGGAACT GGCAAGAGTG TTGCTTGTAG TCCTAAAAAG 
GCCATCAGAG ACCCTAAGAC TGGAAAAGCT GTGCAGGAGA GAAACTCGTA TGCGGTGAGT 
GTGTGGAAGA GAGTGAAAGC CAAGCTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG 
TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT 
CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SMG1 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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