Smg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smg1
Entrez Gene ID	691397
Full Name	SMG1, nonsense mediated mRNA decay associated PI3K related kinase
Synonyms	RGD1563508
General protein information	Preferred Names SMG1, nonsense mediated mRNA decay associated PI3K related kinase Names LOW QUALITY PROTEIN: serine/threonine-protein kinase SMG1 serine/threonine-protein kinase SMG1 SMG1 phosphatidylinositol 3-kinase-related kinase smg-1 homolog, phosphatidylinositol 3-kinase-related kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q35

mRNA and Protein(s)

mRNA	Protein	Name
XM_008759828.2	XP_008758050.1	LOW QUALITY PROTEIN: serine/threonine-protein kinase SMG1 isoform X1
XM_006223459.3	XP_006223521.1	serine/threonine-protein kinase SMG1 isoform X3
XM_002725659.4	XP_002725705.1	serine/threonine-protein kinase SMG1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMG1	XP_851552.2
Bos taurus (cattle)	SMG1	NP_001192844.1
Pan troglodytes (chimpanzee)	SMG1	XP_001152224.2
Homo sapiens (human)	SMG1	NP_055907.3
Rattus norvegicus (Norway rat)	Smg1	XP_002728837.2
Gallus gallus (chicken)	SMG1	XP_414907.3
Mus musculus (house mouse)	Smg1	NP_001026984.1
Macaca mulatta (Rhesus monkey)	LOC693542	XP_002808335.1
Danio rerio (zebrafish)	smg1	NP_001073513.2
Xenopus tropicalis (tropical clawed frog)	smg1	XP_002932267.2

Gene Ontology

The following Smg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa14616	XM_002725659.4 Latest version!	Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ORa14617	XM_006223459.3 Latest version!	Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ORa50597	XM_008759828.2 Latest version!	Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smg1 ( XM_002725659.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_002725705.1 Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X2, mRNA.

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CloneID	ORa14616
Clone ID Related Accession (Same CDS sequence)	XM_002725659.4
Accession Version	XM_002725659.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_002725659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATACTTTA 
AAATACTCCT CATCCAGAGA TAGGGGTGTT TCTTCCTCTT ATGGACTGCA ACCTTCAAAT 
TCAGCTGTGG TGTCTCGGCA AAGGCACGAT GATACCAGAG GCCATGCTGA CATACAGAAT 
GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG 
TCGTTGGGGC AAGAGCTGAG GATTAACAAT GTGACCAGCC CTGAGTTTAC CAGTGTTCAG 
CATGGCAGTC GTGCGTTAGC CACCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCG 
TACTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA 
GACCGAAGAC TGGCTACTGT AAAGCAGCTG AAGGAGTTTA TTCAGCAGCC AGAAAATAAA 
CTGGTACTAG TTAAACAGTT GGATAATATC TTGGCTGCTG TGCATGATGT ACTAAATGAA 
AGTAGTAAGT TGCTTCAGGA GTTGAGGCAA GAGGGAGCTT GCTGTCTTGG TCTTCTCTGT 
GCTTCTCTGA GCTACGAGGC TGAGAAGATC TTCAAGTGGA TTTTTAGCAA ATTTAGCTCA 
TCTGCGAAAG ATGAGGTGAA GCTCCTCTAC CTGTGTGCCA CTTACAGAGC ACTGGAGACT 
GTTGGCGAGA AGAAAGCCTT TTCTTCGGTG ATGCAGCTTG TGATGACCAG CCTACAATCC 
ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAAT GTGTTAAGTG CATTCTTTTG 
GTGGCTCGAT GCTACCCTCA TATTTTCAGC ACTAATTTTA GGGATACAGT TGATATATTA 
GTTGGATGGC ACATAGATCA TACTCAGAAA CCCTCCCTTA CACAGCAGGT GTCTGGGTGG 
TTGCAGAGTT TGGAGCCATT TTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT 
CAATTTTTAG AAGACATGGA GGCATACGCT GAGGACCTCA GCCATGTGGC CTCTGGGGAG 
TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCATTAC CAAAGCTGGC AGCGCTTCTC 
CGTGTGTTCA GTACTGTGGT GAGGAGCATT GGGGAGCGCT TTAGCCCAAT TCGGGGTCCA 
CCAATTACCG AGGCATATGT AACAGATGTT CTATACAGAG TAATGAGATG CGTGACGGCT 
GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT 
TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTCAT TACATATGGA 
TTAGACCAAC TGGAGAATTG CCAGACTTGT GGTACCGATT ATATCATCTC AGTCTTGAAT 
TTGCTCACAT TGATTGTTGA GCAGATAAAT ACAAAACTCC CATCATCATT TGTAGAAAAG 
CTTTTTATAC CATCATCTAA ACTACTATTT CTACGATACC ACAAAGAAAA AGAGGTTGTT 
GCTGTAGCTC ATGCTGTTTA TCAAGCAGTG CTCAGCTTGA AGAACATCCC CGTTCTGGAG 
ACTGCCTATA AATTAATTTT GGGAGAAATG ACTTGTGCAC TAAACAATTT GCTACACAGT 
CTCCAGCTTC CTGATGCCTG TTCTGAGATT AAACATGAGG CTTTTCAGAA TCATGTGTTC 
AACATTGACA ATGCCAACTT TGTAGTCATA TTTGATCTCA GTGCCCTGAC TACAATTGGA 
AATGCCAAAA ACTCATTAAT TGGGATGTGG GCACTGTCTC CAACTGTATT TGCACTGCTG 
AGTAAGAATC TGATGATAGT GCACAGTGAC CTGGCTGTTC ACTTCCCTGC CATTCAGTAT 
GCGGTGCTCT ACACCCTATA TTCCCATTGT ACCAGACACG ATCACTTCAT TTCCAGTAGT 
CTTAGTTCTT CATCCCCTTC CTTATTTGAT GGCGCGGTGA TTAGTACTGT AACTACAGCC 
ACAAAGAAAC ATTTCTCAAT TATATTAAAT CTTTTGGGAA TATTACTAAA GAAAGATAAT 
CTTAACCAGG ACACAAGGAA ACTGTTAATG ACATGGGCTT TGGAAGTAGC TGTATTAATG 
AAGAAGTCTG AAACATATGC ACCTTTATTC TCCCTTCCAT CTTTTCATAA ATTCTCCAAA 
GGCCTTTTAG CTAACACTCT TGTTGAAGAT GTGAATATCT GCTTGCAAGC ATGTAGCAGT 
CTCCATGCTC TGTCCTCTTC CTTGCCAGAT GATCTGCTAC AGAGGTGTGT TGATGTCTGC 
CGTGTTCAGC TTGTCCATAG TGGAACTCGT ATTCGACAGG CCTTTGGAAA GCTGTTGAAG 
TCAATTCCTT TAGATGTTGT ACTAAGCAAT AACAATCACA CAGAGATTCA GGAGATTTCT 
TTAGCATTGA GAAGCCACAT GAGTAAAGCA CCAAGTAACA CGTTCCACCC TCAGGACTTT 
TCTGATGTCA TCAGTTTCAT TTTGTATGGA AACTCTCACC GAACAGGGAA GGACAATTGG 
TTAGAAAGAT TGTTCTATAG CTGCCAGAGG CTGGATAAGC GTGACCAATC CACAATTCCC 
CGCAATCTTC TAAAGACAGA TGCTGTCCTG TGGCAGTGGG CTATTTGGGA AGCTGCACAG 
TTCACTGTTC TTTCAAAGTT GCGAACCCCA CTGGGCAGAG CTCAGGACAC GTTCCAGACA 
ATTGAAGGTA TCATTAGAAG TCTTGCAGCC CACACACTAA ACCCTGACCA GGATGTTAGT 
CAGTGGACCA CTGCAGATAA TGATGAAGGC CATGGGAGCA ATCAGCTTAG ACTTGTTCTT 
CTGCTTCAGT ATCTGGAGAA CCTGGAGAAA CTAATGTATA ACGCCTATGA GGGGTGTGCC 
AATGCATTAA CATCACCTCC CAAGGTCATT AGGACTTTTT TCTATACCAA TCGACAGACT 
TGCCAAGACT GGCTCACTCG GATCCGTCTT TCCATCATGA GGGTCGGATT GTTGGCAGGT 
CAGCCTGCAG TGACAGTGAG GCACGGCTTT GACTTACTTA CTGAAATGAA AACAAATAGT 
CTGTCTCAGG GGAATGAATT GGAAGTGACT ATCATGATGG TGGTAGAAGC GCTGTGTGAG 
CTCCACTGTC CTGAAGCTAT ACAGGGGATT GCTGTCTGGT CATCTTCAGC TGTTGGTAAA 
AACCTTCTCT GGATTAACTC AGTGGCCCAA CAGGCTGAAG GGAGGTTTGA AAAGGCCTCT 
GTGGAGTACC AGGAGCACCT GTGTGCCATG ACAGGTGTTG ATTGCTGCAT CTCCAGCTTT 
GACAAGTCTG TTCTCACATT GGCCAATGCT GGACGTAACA GTGCCAGCCC CAAACACTCT 
CTAAATGGTG AATCCAGAAA AACTGTGCTG TCCAAATCAA TCGACTCTTC CCCTGAAGTT 
ATCAACTACC TAGGAAATAA GGCATGTGAG TGTTACATCT CCATTGCTGA CTGGGCTGCT 
GTGCAGGAGT GGCAGAACGC GGTCCACGAC CTGAAGAAGA ATGCCAACAG CACCTCACTC 
AATCTGAAAG CCGATTTCAA CTACATCAAG TCACTAAGCA GCTTCGAGTC TGGAGAGTTT 
GTTGAATGCA CTGAGCAGTT GGAATTATTG CCAGGAGAAA ATATTAATCT GCTTGCTGGA 
GGGTCAAAAG AAAAAATAGA TATGAAAAAG CTGCTTCCTA ACATGCTAAG CCCAGACCCA 
CGGGAGCTTC AGAAATCCAT TGAAGTCCAA TTGTTGAGAA GCTCCGTGTT CCTGGCAACT 
GCTCTAAACC ATGTGGAACA AGATCAGAAA TGGCAGTCCT TAACCGAAAA TGTGGTAAAG 
TACTTGAAGC AAACCTCACG CATCGCTATT GGACCACTAA GATTGTCTAC GCTAACGGTG 
TCACAGTCCT TGCCAGTACT GAGTACCCTG CAGCTCTACT GCTCCTCTGC ACTGGAGAAC 
ACAGTTTCTA ATAGACTTTC AACAGAGGAC TGTCTTATCC CACTCTTTAG TGATGCTCTT 
CGCTCATGTA AACAGCATGA TGTGCGGCCT TGGATGCAGG CGTTGAGATA TACCATGTAC 
CAGAATCAAC TGTTAGAGAA AATTAAAGAA CAAACAGTAC CAATTAGAAG CCATCTCATG 
GAACTAGGTC TGACAGCTGC AAAATTTGCT AGAAAACGGG GGAATGTGTC ACTGGCAACA 
AGACTGTTGG CACAGTGCAG TGAGGTTCAG CTGGGAAAGA CAACCACTGC CCAGGACTTA 
GTCCAGCATT TTAAAAAACT GTCTACTCAA GGTCAAGTGG ATGAAAAATG GGGGCCTGAG 
CTTGATATTG AAAAAACCAA ATTGTTATAC ACAGCAGGCC AGTCAACACA CGCAATGGAA 
ATGTTGAGTT CTTGTGCTAT ATCTTTCTGC AAATCTGCGA AAGCTGAATA TGCTGTGGCT 
AAGTCAATTC TGACCCTGGC TAAATGGGTC CAGGCAGAAT GGAAAGAGAT TTCGGGACAG 
CTGAGGCAGG TGTACCGAGC TCAGCAGCAG CAGAACCTTT CAGGCCTTTC TACTTTGTCT 
AGGAATATAC TCGCTCTTAT AGAGCTACCG TCTGCTAATA CAGTGGGAGA AGAGCACCCT 
CGGATTGAGA GTGAGTCTAC AGTGCATATT GGAGTTGGAG AACCTGACTT CATTTTGGGA 
CAGTTGTATC ACCTGTCTTC AGTGCAGGCA CCTGAAGTAG CCAAATCTTG GGCAGCATTG 
GCTAGTTGGG CTTATAGGTG GGGCAGAAAG GTGGTTGACA ATGCCAGTCA GGGAGAAGGT 
GTTCGTCTGT TGCCTAGAGA AAAATCTGAA GTTCAAAATC TGCTTCCAGA TACTATAACT 
GAAGAAGAGA AAGAGAGAAT ATATGGTATT CTTGGACAAG CAGTATGTCG GCCAGCAGGA 
ATTCAGGATG AAGATATAAC ACTACAGATA ACAGAGAGTG AAGATAATGA GGATGATGAC 
ATGGTCGATG TTATCTGGCG GCAGTTAATA TCAAGCTGTC CCTGGCTTTC TGAACTGGAT 
GAAAATGCAA CGGAAGGTGT TATTAAAGTG TGGAGGAAAG TTGTGGACAG AATCTTCAGC 
CTGTACAAAT TGTCTTGCAG TGCATATTTT ACTTTCCTTA AGCTGAATGC TGGTCAGGTT 
CTGTTAGATG AGGATGACCC CAGGCTCCAT TTAAGTCACA GAGCTGAGCA GAGCACTGAT 
GATGTGATTG TGATGGCTAC CCTGCGACTG CTCAGATTGC TAGTGAAGCA TGCTGGTGAG 
CTTCGGCAGT ATCTGGAACA TGGCTTGGAG ACAACCCCTA CTGCTCCATG GAGAGGAATT 
ATTCCACAGC TTTTCTCACG CTTAAACCAC CCTGAAGTGT ATGTACGCCA GAGTATTTGT 
AACCTCCTTT GCCGTGTAGC CCAAGATTCC CCTCACCTCA TATTGTATCC TGCAATAGTA 
GGAACCATAT CACTTAGCAG TGAATCCCAG GCTTCAGGAA ATAAGTATTC CTCTGCAATC 
CCAACTTTAC TTGGAAATAT TCAAGGAGAA GAACTGCTGG TTTCTGAATG TGAAGGAGGA 
AGTCCTCCTG CTTCTCAGGA TAGCAACAAA GATGAACCTA AAAGTGGTTT AAATGAAGAT 
CAGGCCATGA TGCAGGATTG CTACAGCAAA ATTGTAGATA AACTATCCTC TGCAAATCCA 
ACGATGGTTT TACAGGTCCA GATGCTTGTG GCAGAGCTAC GTAGGGTCAC TGTACTCTGG 
GATGAGCTCT GGCTGGGAGT TTTGTTACAG CAGCATATGT ACGTCCTGAG GCGAATTCAG 
CAGCTGGAGG ATGAGGTGAA GAGAGTCCAG AACAACAACA CCTTGCGCAA AGAAGAGAAA 
ATTGCCATCA TGCGAGAGAA GCACACAGCA CTGATGAAGC CCATCGTGTT TGCTTTGGAG 
CACGTGCGGA GCATCACTGC TGCCCCTGCA GAGACCCCTC ATGAAAAGTG GTTTCAGGAT 
AACTATGGTG ATGCCATTGA CAATGCCCTT GAAAAACTAA AGACCCCATC CAACCCTGCC 
AAGCCTGGAA GCAGCTGGAT TCCATTTAAA GAGATAATGC TGAGTTTGCA ACAGAGAGCG 
CAGAAACGAG CAAGCTACAT CTTGCGACTT GATGAGATCA GTCCTTGGTT GGCTGCCATG 
ACTAACACTG AAATTGCACT CCCTGGAGAA GTCTCTGCCA GAGACACTGT GACAATTCAC 
AGTGTGGGTG GAACTATCAC TATCTTACCA ACTAAAACCA AGCCCAAGAA ACTCCTCTTT 
CTTGGATCAG ATGGAAAGAG CTATCCTTAC CTTTTCAAAG GTCTAGAAGA TTTGCATCTG 
GATGAGAGAA TCATGCAGTT TTTATCTATT GTGAATACTA TGTTTGCTAC AATTAATCGG 
CAGGAAACAC CCCGATTCCA TGCACGGCAC TATTCTGTAA CACCACTGGG GACGAGATCG 
GGGCTGATCC AGTGGGTGGA TGGAGCCACA CCATTGTTCG GGCTTTACAA ACGATGGCAA 
CAGCGGGAGG CTGCCTTACA AGCACAAAAG GCCCAAGATT CCTACCAGAC TCCTCAGAAT 
CCCAGCATTG TACCGCGTCC TAGTGAACTT TACTATAGTA AAATTGGTCC TGCTTTGAAA 
ACAGTTGGCC TTAGCCTGGA TGTGTCAAGG AGGGATTGGC CCCTCCATGT GATGAAGGCA 
GTGTTGGAGG AGTTAATGGA GGCCACACCC CCAAACCTTC TGGCCAAAGA ACTCTGGTCA 
TCTTGCACCA CTCCCGATGA GTGGTGGAGA GTCACACAGT CCTATGCAAG ATCCACCGCT 
GTCATGTCTA TGGTTGGCTA CATAATTGGC CTTGGAGACA GACATCTGGA TAACGTCCTC 
ATAGATATGA CAACTGGAGA AGTAGTTCAC ATAGATTATA ATGTTTGCTT TGAAAAAGGT 
AAAAGCCTTA GAGTTCCTGA GAAAGTGCCT TTTCGAATGA CACAAAACAT TGAAACAGCA 
CTGGGTGTAA CTGGAGTAGA AGGTGTTTTT AGGCTTTCCT GTGAGCAGGT TCTCCACATC 
ATGCGACGAG GCCGAGAGAC TCTGCTGACA CTCTTGGAGG CTTTTGTGTA TGACCCACTG 
GTGGACTGGA CAGCCGGCGG TGAGGCTGGG TTTGCAGGTG CTGTCTATGG TGGAGGTGGC 
CAACAGGCCG AGAGCAAGCA GAGTAAGCGA GAGATGGAGC GTGAGATTAC CCGCAGCCTC 
TTTTCTTCCA GAGTTGCTGA GATTAAGGTG AACTGGTTTA AGAACCGGGA TGAGATGCTA 
GTTGTGCTTC CCAAGCTGGA CAGCAGCTTA GATGAATACC TAAGCCTGCA GGAGCAGCTG 
ACAGATGTTG AAAAGCTGCA GGGCAAACTG CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT 
GAAGGAGTGG ATCATCCATC TCGCACTCTG CAACACAGAT ATTCTGAGCA TACTCAACTA 
CAGACCCAGC AAAGAGCTGT TCAGGAAGCA ATCCAGGTGA AGCTGAATGA GTTTGAACAA 
TGGATAACAC ATTATCAGGC TGCATTCAAT AACTTAGAAG CAACTCAGCT TGCAAGTTTG 
CTTCAAGAGA TAAGCACACA AATGGACCTT GGTCCCCCAA GCTATGTTCC AGCAACAGCT 
TTTCTACAGA ATGCTGGCCA AGCACACCTC ATCAGCCAGT GTGAGCAGCT AGAGGGGGAA 
GTTGGTACTC TGCTGCAGCA GAGGCGCTCT GTGCTCCGTG GCTGTCTGGA ACAGCTGCAT 
CACTATGCAA CTGTCGCTCT GCAGTATCCG AAAGCAATAT TCCAGAAACA TCGAATTGAA 
CAGTGGAAGG CCTGGATGGA AGAGCTCATC TGCAACACCA CAGTAGAACG CTGCCAAGAA 
CTCTATAGGA AATATGAGAT GCAGTATGCT CCTCAGCCCC CTCCAACAGT CTGTCAGTTC 
ATTACTGCCA CTGAAATGAC CCTTCAGCGG TATGCAGCAG ACATAAACAG CAGACTTATT 
AGACAAGTGG AACGCTTGAA GCAAGAAGCT GTCACTGTGC CAGTTTGTGA GGATCAGTTG 
AAAGAAATTG AGCGTTGCAT TAAAGTTTTT CTTCATGAGA ATGGAGAAGA AGGATCTCTG 
AGTCTAGCAA GTGTCATTAT TTCTGCCCTT TGTACTCTTA CAAGGCGCAA CCTTATGATG 
GAAGGTGCAG CTTCAAGTGC TGGAGAGCAG CTGGTTGATC TGACATCCAG AGATGGAGCC 
TGGTTCCTAG AGGAGCTGTG TAGTATGAGT GGAAATGTCA CCTGCTTGGT GCAGTTACTG 
AAGCAGTGCC ACCTGGTACC ACAGGACTTG GATATTCCCA ACCCAGTGGA AGCATCTGAG 
GCTGTTCATT TAGCCAATGG AGTGTATACA TCTCTGCAGG AATTAAATTC AAATTTCCGG 
CAAATCATAT TCCCAGAAGC TCTTAGATGT TTAATGAAGG GGGAGTGCAC TTTAGAAAGT 
ATGCTACATG AACTAGACAG TCTCATCGAG CAGACTACTG ATGGAGTTCC TCTGCAGACT 
CTGGTGGAGT CGCTTCAAGC CTACTTAAGA AACACAGCCA TGGGACTTGA AGAAGAAACT 
CATGCTCATT ATATTGATGT TGCCAGAATG CTACATGCTC AATATGGTGA ATTAATCCAA 
CCAAGAAATG GTTCAGTTGA TGAAACACCA AAAATGTCAG CTGGCCAGAT GCTTTTGGTA 
GCATTTGATG GCATGTTTGC CCAAGTTGAA ACTGCTTTTG GCTTATTAGT TGAGAAGTTA 
AACAAGATGG AGATTCCCGT TGCTTGGAGG AAGATTGACA TTATCCGGGA AGCCAGGAGC 
ACCCAGGCCA ATTTTTTTGA TGATGACAAT CACCGGCAGG TGCTAGAAGA GATTTTCTTT 
TTAAAAAGAC TGCAGACAAT TAAGGAGTTC TTCAGGCTCT GTGGTACCTT TTCTAAAACA 
TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTAA ATGGACCTGT TCAGATTGTC 
AATGTGAAAA CCCTTTTTAG AAATTCTTGC TTTAGTGAAG ACCAAATGGC CAAGCCTATC 
AAGGCCTTTA CAGCTGACTT TGTAAGACAG CTCTTGATAG GACTCCCAAA CCAAGCCCTC 
GGACTCACAT TGTGTAGCTT TATCAGTGCC CTGGGCGTAG ACATCATTGC TCAGGTAGAG 
GCAAAGGATT TCGGAGCTGA AAGCAAAGTC TCTGTGGACG ATCTGTGTAA GAAAGCTGTG 
GAGCACAACA TCCAGGTTGG GAGATTCTCA CAGTTGGTGA TGAACAGAGC TACCGTGTTA 
GCCAGCTCCT ATGATACAGC CTGGAAGAAG CATGACCTGG TGCGCAGGCT GGAAACCAGC 
ATTTCTTCCT GTAAGACAAG CTTACAGCGG GTGCAACTGC ACATTGCCAT GTTCCAGTGG 
CAACATGAAG ACCTCCTTAT CAACAGACCA CAAGCCATGT CAGTCACCCC ACCTCGGTCT 
GCAATCCTGA CCAGCATGAA GAAGAAACTG CATGCTCTCA GTCAGATTGA GACTTCAATC 
GGGACTGTTC AGGAGAAACT AGCAGCTCTT GAAGCAAGCA TTGAGCAGCG GCTGAAGTGG 
GCAGGTGGTG CCAATCCTGC CCTGGCACCA GTACTACAAG ATTTTGAAGC AACAATAGCT 
GAAAGAAGAA ATCTTGTTCT CAAAGAGAGC CAGAGAGCAA ACCAGGTCAC TTTTCTCTGC 
AGCAATATCA TTCATTTTGA AAGTCTACGA ACAAGAACTG CCGAAGCCTT AAGCCTGGAT 
GCTGCCTTAT TTGAACTCAT CAAACGGTGT CAGCAGATGT GTTCATTTGC ATCACAGTTC 
AACAGTTCAG TTTCTGAGTT AGAGCTTCGT TTATTACAGA GAGTGGACAC GACTCTTGAA 
CATCCTATTG GCAGTTCTGA GTGGCTTTTA TCAGCACATA AACAGTTGAC CCAGGATATG 
TCCACCCAAA GGGCTGTTCA GACAGAGAAG GAGCAGCAGA TCGAGACTGT TTGTGAGACC 
ATCCAGAGCC TGGTTGACAG CGTAAAGACT GTGCTCACGG GTCACAACCG GCAGCTTGGA 
GATGTAAAAC ATCTCCTGAA AGCCATGGCT AAGGACGAAG AGGCCGCTCT AGCAGATGCT 
GAAGATATCC CCTATGAGAG CAGTGTCAGG CAGTTCCTGG CCGAGTACAA ATCATGGCAA 
GACAACATTC AGACGGTTTT GTTCACACTG GTCCAGGCCA TGGGTCAGGT TCGAAGTCAA 
GAACATGTTG AAATGCTGCA GGAAATCACT CCCACCTTGA AAGAGCTGAA GACACAAAGC 
CAGAGTATCT ATAATAATTT AGTGAGTTTT GCATCACCCT TAGTCACCGA CGCAGCAAAC 
GAATGTTCAA GTCCAACATC ATCTGCTACT TACCAGCCGT CCTTTGCTGC AGCGGTTAGG 
AGTAACACTG GCCAGAAGAC TCAGCCTGAT GTCATGTCTC AGAATGCTAA AAAGCTGATT 
CAGAAAAATC TGGCTACATC AGCAGATACT CCACCAAGCA CCATCCCAGG AACTGGCAAG 
AGCATCGCCT GTAGTCCCAA AAAGGCTATC AGAGACCCTA AAACTGGGAA AGCTGTGCAA 
GAGAGAAACT CCTATGCAGT GAGTGTGTGG AAGAGAGTGA AAGCCAAGCT AGAAGGCCGA 
GATGTTGATC CAAACAGGAG GATGTCAGTT GCTGAGCAGG TTGACTATGT CATTAAGGAA 
GCAACTAATC TAGATAACTT GGCTCAGCTG TATGAAGGTT GGACAGCCTG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002725705.1
CDS	328..11304
Translation	MSRRAPGSRLSSGGGGTKYPRSWNDWQPRTDSASADPDTLKYSSSRDRGVSSSYGLQPSNSAVVSRQRHDDTRGHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRINNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYRALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFQNHVFNIDNANFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFSKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSAVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKSIDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKKNANSTSLNLKADFNYIKSLSSFESGEFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVFLATALNHVEQDQKWQSLTENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSDALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWVQAEWKEISGQLRQVYRAQQQQNLSGLSTLSRNILALIELPSANTVGEEHPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEDDDMVDVIWRQLISSCPWLSELDENATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVLLDEDDPRLHLSHRAEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKYSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIDNALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLDEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPSIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSRTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGTLLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKAWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPVEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGECTLESMLHELDSLIEQTTDGVPLQTLVESLQAYLRNTAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPVAWRKIDIIREARSTQANFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQVGRFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPRSAILTSMKKKLHALSQIETSIGTVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRANQVTFLCSNIIHFESLRTRTAEALSLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAVQTEKEQQIETVCETIQSLVDSVKTVLTGHNRQLGDVKHLLKAMAKDEEAALADAEDIPYESSVRQFLAEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDAANECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNAKKLIQKNLATSADTPPSTIPGTGKSIACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_002725659.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002725659.4

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATACTTTA 
AAATACTCCT CATCCAGAGA TAGGGGTGTT TCTTCCTCTT ATGGACTGCA ACCTTCAAAT 
TCAGCTGTGG TGTCTCGGCA AAGGCACGAT GATACCAGAG GCCATGCTGA CATACAGAAT 
GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG 
TCGTTGGGGC AAGAGCTGAG GATTAACAAT GTGACCAGCC CTGAGTTTAC CAGTGTTCAG 
CATGGCAGTC GTGCGTTAGC CACCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCG 
TACTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA 
GACCGAAGAC TGGCTACTGT AAAGCAGCTG AAGGAGTTTA TTCAGCAGCC AGAAAATAAA 
CTGGTACTAG TTAAACAGTT GGATAATATC TTGGCTGCTG TGCATGATGT ACTAAATGAA 
AGTAGTAAGT TGCTTCAGGA GTTGAGGCAA GAGGGAGCTT GCTGTCTTGG TCTTCTCTGT 
GCTTCTCTGA GCTACGAGGC TGAGAAGATC TTCAAGTGGA TTTTTAGCAA ATTTAGCTCA 
TCTGCGAAAG ATGAGGTGAA GCTCCTCTAC CTGTGTGCCA CTTACAGAGC ACTGGAGACT 
GTTGGCGAGA AGAAAGCCTT TTCTTCGGTG ATGCAGCTTG TGATGACCAG CCTACAATCC 
ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAAT GTGTTAAGTG CATTCTTTTG 
GTGGCTCGAT GCTACCCTCA TATTTTCAGC ACTAATTTTA GGGATACAGT TGATATATTA 
GTTGGATGGC ACATAGATCA TACTCAGAAA CCCTCCCTTA CACAGCAGGT GTCTGGGTGG 
TTGCAGAGTT TGGAGCCATT TTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT 
CAATTTTTAG AAGACATGGA GGCATACGCT GAGGACCTCA GCCATGTGGC CTCTGGGGAG 
TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCATTAC CAAAGCTGGC AGCGCTTCTC 
CGTGTGTTCA GTACTGTGGT GAGGAGCATT GGGGAGCGCT TTAGCCCAAT TCGGGGTCCA 
CCAATTACCG AGGCATATGT AACAGATGTT CTATACAGAG TAATGAGATG CGTGACGGCT 
GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT 
TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTCAT TACATATGGA 
TTAGACCAAC TGGAGAATTG CCAGACTTGT GGTACCGATT ATATCATCTC AGTCTTGAAT 
TTGCTCACAT TGATTGTTGA GCAGATAAAT ACAAAACTCC CATCATCATT TGTAGAAAAG 
CTTTTTATAC CATCATCTAA ACTACTATTT CTACGATACC ACAAAGAAAA AGAGGTTGTT 
GCTGTAGCTC ATGCTGTTTA TCAAGCAGTG CTCAGCTTGA AGAACATCCC CGTTCTGGAG 
ACTGCCTATA AATTAATTTT GGGAGAAATG ACTTGTGCAC TAAACAATTT GCTACACAGT 
CTCCAGCTTC CTGATGCCTG TTCTGAGATT AAACATGAGG CTTTTCAGAA TCATGTGTTC 
AACATTGACA ATGCCAACTT TGTAGTCATA TTTGATCTCA GTGCCCTGAC TACAATTGGA 
AATGCCAAAA ACTCATTAAT TGGGATGTGG GCACTGTCTC CAACTGTATT TGCACTGCTG 
AGTAAGAATC TGATGATAGT GCACAGTGAC CTGGCTGTTC ACTTCCCTGC CATTCAGTAT 
GCGGTGCTCT ACACCCTATA TTCCCATTGT ACCAGACACG ATCACTTCAT TTCCAGTAGT 
CTTAGTTCTT CATCCCCTTC CTTATTTGAT GGCGCGGTGA TTAGTACTGT AACTACAGCC 
ACAAAGAAAC ATTTCTCAAT TATATTAAAT CTTTTGGGAA TATTACTAAA GAAAGATAAT 
CTTAACCAGG ACACAAGGAA ACTGTTAATG ACATGGGCTT TGGAAGTAGC TGTATTAATG 
AAGAAGTCTG AAACATATGC ACCTTTATTC TCCCTTCCAT CTTTTCATAA ATTCTCCAAA 
GGCCTTTTAG CTAACACTCT TGTTGAAGAT GTGAATATCT GCTTGCAAGC ATGTAGCAGT 
CTCCATGCTC TGTCCTCTTC CTTGCCAGAT GATCTGCTAC AGAGGTGTGT TGATGTCTGC 
CGTGTTCAGC TTGTCCATAG TGGAACTCGT ATTCGACAGG CCTTTGGAAA GCTGTTGAAG 
TCAATTCCTT TAGATGTTGT ACTAAGCAAT AACAATCACA CAGAGATTCA GGAGATTTCT 
TTAGCATTGA GAAGCCACAT GAGTAAAGCA CCAAGTAACA CGTTCCACCC TCAGGACTTT 
TCTGATGTCA TCAGTTTCAT TTTGTATGGA AACTCTCACC GAACAGGGAA GGACAATTGG 
TTAGAAAGAT TGTTCTATAG CTGCCAGAGG CTGGATAAGC GTGACCAATC CACAATTCCC 
CGCAATCTTC TAAAGACAGA TGCTGTCCTG TGGCAGTGGG CTATTTGGGA AGCTGCACAG 
TTCACTGTTC TTTCAAAGTT GCGAACCCCA CTGGGCAGAG CTCAGGACAC GTTCCAGACA 
ATTGAAGGTA TCATTAGAAG TCTTGCAGCC CACACACTAA ACCCTGACCA GGATGTTAGT 
CAGTGGACCA CTGCAGATAA TGATGAAGGC CATGGGAGCA ATCAGCTTAG ACTTGTTCTT 
CTGCTTCAGT ATCTGGAGAA CCTGGAGAAA CTAATGTATA ACGCCTATGA GGGGTGTGCC 
AATGCATTAA CATCACCTCC CAAGGTCATT AGGACTTTTT TCTATACCAA TCGACAGACT 
TGCCAAGACT GGCTCACTCG GATCCGTCTT TCCATCATGA GGGTCGGATT GTTGGCAGGT 
CAGCCTGCAG TGACAGTGAG GCACGGCTTT GACTTACTTA CTGAAATGAA AACAAATAGT 
CTGTCTCAGG GGAATGAATT GGAAGTGACT ATCATGATGG TGGTAGAAGC GCTGTGTGAG 
CTCCACTGTC CTGAAGCTAT ACAGGGGATT GCTGTCTGGT CATCTTCAGC TGTTGGTAAA 
AACCTTCTCT GGATTAACTC AGTGGCCCAA CAGGCTGAAG GGAGGTTTGA AAAGGCCTCT 
GTGGAGTACC AGGAGCACCT GTGTGCCATG ACAGGTGTTG ATTGCTGCAT CTCCAGCTTT 
GACAAGTCTG TTCTCACATT GGCCAATGCT GGACGTAACA GTGCCAGCCC CAAACACTCT 
CTAAATGGTG AATCCAGAAA AACTGTGCTG TCCAAATCAA TCGACTCTTC CCCTGAAGTT 
ATCAACTACC TAGGAAATAA GGCATGTGAG TGTTACATCT CCATTGCTGA CTGGGCTGCT 
GTGCAGGAGT GGCAGAACGC GGTCCACGAC CTGAAGAAGA ATGCCAACAG CACCTCACTC 
AATCTGAAAG CCGATTTCAA CTACATCAAG TCACTAAGCA GCTTCGAGTC TGGAGAGTTT 
GTTGAATGCA CTGAGCAGTT GGAATTATTG CCAGGAGAAA ATATTAATCT GCTTGCTGGA 
GGGTCAAAAG AAAAAATAGA TATGAAAAAG CTGCTTCCTA ACATGCTAAG CCCAGACCCA 
CGGGAGCTTC AGAAATCCAT TGAAGTCCAA TTGTTGAGAA GCTCCGTGTT CCTGGCAACT 
GCTCTAAACC ATGTGGAACA AGATCAGAAA TGGCAGTCCT TAACCGAAAA TGTGGTAAAG 
TACTTGAAGC AAACCTCACG CATCGCTATT GGACCACTAA GATTGTCTAC GCTAACGGTG 
TCACAGTCCT TGCCAGTACT GAGTACCCTG CAGCTCTACT GCTCCTCTGC ACTGGAGAAC 
ACAGTTTCTA ATAGACTTTC AACAGAGGAC TGTCTTATCC CACTCTTTAG TGATGCTCTT 
CGCTCATGTA AACAGCATGA TGTGCGGCCT TGGATGCAGG CGTTGAGATA TACCATGTAC 
CAGAATCAAC TGTTAGAGAA AATTAAAGAA CAAACAGTAC CAATTAGAAG CCATCTCATG 
GAACTAGGTC TGACAGCTGC AAAATTTGCT AGAAAACGGG GGAATGTGTC ACTGGCAACA 
AGACTGTTGG CACAGTGCAG TGAGGTTCAG CTGGGAAAGA CAACCACTGC CCAGGACTTA 
GTCCAGCATT TTAAAAAACT GTCTACTCAA GGTCAAGTGG ATGAAAAATG GGGGCCTGAG 
CTTGATATTG AAAAAACCAA ATTGTTATAC ACAGCAGGCC AGTCAACACA CGCAATGGAA 
ATGTTGAGTT CTTGTGCTAT ATCTTTCTGC AAATCTGCGA AAGCTGAATA TGCTGTGGCT 
AAGTCAATTC TGACCCTGGC TAAATGGGTC CAGGCAGAAT GGAAAGAGAT TTCGGGACAG 
CTGAGGCAGG TGTACCGAGC TCAGCAGCAG CAGAACCTTT CAGGCCTTTC TACTTTGTCT 
AGGAATATAC TCGCTCTTAT AGAGCTACCG TCTGCTAATA CAGTGGGAGA AGAGCACCCT 
CGGATTGAGA GTGAGTCTAC AGTGCATATT GGAGTTGGAG AACCTGACTT CATTTTGGGA 
CAGTTGTATC ACCTGTCTTC AGTGCAGGCA CCTGAAGTAG CCAAATCTTG GGCAGCATTG 
GCTAGTTGGG CTTATAGGTG GGGCAGAAAG GTGGTTGACA ATGCCAGTCA GGGAGAAGGT 
GTTCGTCTGT TGCCTAGAGA AAAATCTGAA GTTCAAAATC TGCTTCCAGA TACTATAACT 
GAAGAAGAGA AAGAGAGAAT ATATGGTATT CTTGGACAAG CAGTATGTCG GCCAGCAGGA 
ATTCAGGATG AAGATATAAC ACTACAGATA ACAGAGAGTG AAGATAATGA GGATGATGAC 
ATGGTCGATG TTATCTGGCG GCAGTTAATA TCAAGCTGTC CCTGGCTTTC TGAACTGGAT 
GAAAATGCAA CGGAAGGTGT TATTAAAGTG TGGAGGAAAG TTGTGGACAG AATCTTCAGC 
CTGTACAAAT TGTCTTGCAG TGCATATTTT ACTTTCCTTA AGCTGAATGC TGGTCAGGTT 
CTGTTAGATG AGGATGACCC CAGGCTCCAT TTAAGTCACA GAGCTGAGCA GAGCACTGAT 
GATGTGATTG TGATGGCTAC CCTGCGACTG CTCAGATTGC TAGTGAAGCA TGCTGGTGAG 
CTTCGGCAGT ATCTGGAACA TGGCTTGGAG ACAACCCCTA CTGCTCCATG GAGAGGAATT 
ATTCCACAGC TTTTCTCACG CTTAAACCAC CCTGAAGTGT ATGTACGCCA GAGTATTTGT 
AACCTCCTTT GCCGTGTAGC CCAAGATTCC CCTCACCTCA TATTGTATCC TGCAATAGTA 
GGAACCATAT CACTTAGCAG TGAATCCCAG GCTTCAGGAA ATAAGTATTC CTCTGCAATC 
CCAACTTTAC TTGGAAATAT TCAAGGAGAA GAACTGCTGG TTTCTGAATG TGAAGGAGGA 
AGTCCTCCTG CTTCTCAGGA TAGCAACAAA GATGAACCTA AAAGTGGTTT AAATGAAGAT 
CAGGCCATGA TGCAGGATTG CTACAGCAAA ATTGTAGATA AACTATCCTC TGCAAATCCA 
ACGATGGTTT TACAGGTCCA GATGCTTGTG GCAGAGCTAC GTAGGGTCAC TGTACTCTGG 
GATGAGCTCT GGCTGGGAGT TTTGTTACAG CAGCATATGT ACGTCCTGAG GCGAATTCAG 
CAGCTGGAGG ATGAGGTGAA GAGAGTCCAG AACAACAACA CCTTGCGCAA AGAAGAGAAA 
ATTGCCATCA TGCGAGAGAA GCACACAGCA CTGATGAAGC CCATCGTGTT TGCTTTGGAG 
CACGTGCGGA GCATCACTGC TGCCCCTGCA GAGACCCCTC ATGAAAAGTG GTTTCAGGAT 
AACTATGGTG ATGCCATTGA CAATGCCCTT GAAAAACTAA AGACCCCATC CAACCCTGCC 
AAGCCTGGAA GCAGCTGGAT TCCATTTAAA GAGATAATGC TGAGTTTGCA ACAGAGAGCG 
CAGAAACGAG CAAGCTACAT CTTGCGACTT GATGAGATCA GTCCTTGGTT GGCTGCCATG 
ACTAACACTG AAATTGCACT CCCTGGAGAA GTCTCTGCCA GAGACACTGT GACAATTCAC 
AGTGTGGGTG GAACTATCAC TATCTTACCA ACTAAAACCA AGCCCAAGAA ACTCCTCTTT 
CTTGGATCAG ATGGAAAGAG CTATCCTTAC CTTTTCAAAG GTCTAGAAGA TTTGCATCTG 
GATGAGAGAA TCATGCAGTT TTTATCTATT GTGAATACTA TGTTTGCTAC AATTAATCGG 
CAGGAAACAC CCCGATTCCA TGCACGGCAC TATTCTGTAA CACCACTGGG GACGAGATCG 
GGGCTGATCC AGTGGGTGGA TGGAGCCACA CCATTGTTCG GGCTTTACAA ACGATGGCAA 
CAGCGGGAGG CTGCCTTACA AGCACAAAAG GCCCAAGATT CCTACCAGAC TCCTCAGAAT 
CCCAGCATTG TACCGCGTCC TAGTGAACTT TACTATAGTA AAATTGGTCC TGCTTTGAAA 
ACAGTTGGCC TTAGCCTGGA TGTGTCAAGG AGGGATTGGC CCCTCCATGT GATGAAGGCA 
GTGTTGGAGG AGTTAATGGA GGCCACACCC CCAAACCTTC TGGCCAAAGA ACTCTGGTCA 
TCTTGCACCA CTCCCGATGA GTGGTGGAGA GTCACACAGT CCTATGCAAG ATCCACCGCT 
GTCATGTCTA TGGTTGGCTA CATAATTGGC CTTGGAGACA GACATCTGGA TAACGTCCTC 
ATAGATATGA CAACTGGAGA AGTAGTTCAC ATAGATTATA ATGTTTGCTT TGAAAAAGGT 
AAAAGCCTTA GAGTTCCTGA GAAAGTGCCT TTTCGAATGA CACAAAACAT TGAAACAGCA 
CTGGGTGTAA CTGGAGTAGA AGGTGTTTTT AGGCTTTCCT GTGAGCAGGT TCTCCACATC 
ATGCGACGAG GCCGAGAGAC TCTGCTGACA CTCTTGGAGG CTTTTGTGTA TGACCCACTG 
GTGGACTGGA CAGCCGGCGG TGAGGCTGGG TTTGCAGGTG CTGTCTATGG TGGAGGTGGC 
CAACAGGCCG AGAGCAAGCA GAGTAAGCGA GAGATGGAGC GTGAGATTAC CCGCAGCCTC 
TTTTCTTCCA GAGTTGCTGA GATTAAGGTG AACTGGTTTA AGAACCGGGA TGAGATGCTA 
GTTGTGCTTC CCAAGCTGGA CAGCAGCTTA GATGAATACC TAAGCCTGCA GGAGCAGCTG 
ACAGATGTTG AAAAGCTGCA GGGCAAACTG CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT 
GAAGGAGTGG ATCATCCATC TCGCACTCTG CAACACAGAT ATTCTGAGCA TACTCAACTA 
CAGACCCAGC AAAGAGCTGT TCAGGAAGCA ATCCAGGTGA AGCTGAATGA GTTTGAACAA 
TGGATAACAC ATTATCAGGC TGCATTCAAT AACTTAGAAG CAACTCAGCT TGCAAGTTTG 
CTTCAAGAGA TAAGCACACA AATGGACCTT GGTCCCCCAA GCTATGTTCC AGCAACAGCT 
TTTCTACAGA ATGCTGGCCA AGCACACCTC ATCAGCCAGT GTGAGCAGCT AGAGGGGGAA 
GTTGGTACTC TGCTGCAGCA GAGGCGCTCT GTGCTCCGTG GCTGTCTGGA ACAGCTGCAT 
CACTATGCAA CTGTCGCTCT GCAGTATCCG AAAGCAATAT TCCAGAAACA TCGAATTGAA 
CAGTGGAAGG CCTGGATGGA AGAGCTCATC TGCAACACCA CAGTAGAACG CTGCCAAGAA 
CTCTATAGGA AATATGAGAT GCAGTATGCT CCTCAGCCCC CTCCAACAGT CTGTCAGTTC 
ATTACTGCCA CTGAAATGAC CCTTCAGCGG TATGCAGCAG ACATAAACAG CAGACTTATT 
AGACAAGTGG AACGCTTGAA GCAAGAAGCT GTCACTGTGC CAGTTTGTGA GGATCAGTTG 
AAAGAAATTG AGCGTTGCAT TAAAGTTTTT CTTCATGAGA ATGGAGAAGA AGGATCTCTG 
AGTCTAGCAA GTGTCATTAT TTCTGCCCTT TGTACTCTTA CAAGGCGCAA CCTTATGATG 
GAAGGTGCAG CTTCAAGTGC TGGAGAGCAG CTGGTTGATC TGACATCCAG AGATGGAGCC 
TGGTTCCTAG AGGAGCTGTG TAGTATGAGT GGAAATGTCA CCTGCTTGGT GCAGTTACTG 
AAGCAGTGCC ACCTGGTACC ACAGGACTTG GATATTCCCA ACCCAGTGGA AGCATCTGAG 
GCTGTTCATT TAGCCAATGG AGTGTATACA TCTCTGCAGG AATTAAATTC AAATTTCCGG 
CAAATCATAT TCCCAGAAGC TCTTAGATGT TTAATGAAGG GGGAGTGCAC TTTAGAAAGT 
ATGCTACATG AACTAGACAG TCTCATCGAG CAGACTACTG ATGGAGTTCC TCTGCAGACT 
CTGGTGGAGT CGCTTCAAGC CTACTTAAGA AACACAGCCA TGGGACTTGA AGAAGAAACT 
CATGCTCATT ATATTGATGT TGCCAGAATG CTACATGCTC AATATGGTGA ATTAATCCAA 
CCAAGAAATG GTTCAGTTGA TGAAACACCA AAAATGTCAG CTGGCCAGAT GCTTTTGGTA 
GCATTTGATG GCATGTTTGC CCAAGTTGAA ACTGCTTTTG GCTTATTAGT TGAGAAGTTA 
AACAAGATGG AGATTCCCGT TGCTTGGAGG AAGATTGACA TTATCCGGGA AGCCAGGAGC 
ACCCAGGCCA ATTTTTTTGA TGATGACAAT CACCGGCAGG TGCTAGAAGA GATTTTCTTT 
TTAAAAAGAC TGCAGACAAT TAAGGAGTTC TTCAGGCTCT GTGGTACCTT TTCTAAAACA 
TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTAA ATGGACCTGT TCAGATTGTC 
AATGTGAAAA CCCTTTTTAG AAATTCTTGC TTTAGTGAAG ACCAAATGGC CAAGCCTATC 
AAGGCCTTTA CAGCTGACTT TGTAAGACAG CTCTTGATAG GACTCCCAAA CCAAGCCCTC 
GGACTCACAT TGTGTAGCTT TATCAGTGCC CTGGGCGTAG ACATCATTGC TCAGGTAGAG 
GCAAAGGATT TCGGAGCTGA AAGCAAAGTC TCTGTGGACG ATCTGTGTAA GAAAGCTGTG 
GAGCACAACA TCCAGGTTGG GAGATTCTCA CAGTTGGTGA TGAACAGAGC TACCGTGTTA 
GCCAGCTCCT ATGATACAGC CTGGAAGAAG CATGACCTGG TGCGCAGGCT GGAAACCAGC 
ATTTCTTCCT GTAAGACAAG CTTACAGCGG GTGCAACTGC ACATTGCCAT GTTCCAGTGG 
CAACATGAAG ACCTCCTTAT CAACAGACCA CAAGCCATGT CAGTCACCCC ACCTCGGTCT 
GCAATCCTGA CCAGCATGAA GAAGAAACTG CATGCTCTCA GTCAGATTGA GACTTCAATC 
GGGACTGTTC AGGAGAAACT AGCAGCTCTT GAAGCAAGCA TTGAGCAGCG GCTGAAGTGG 
GCAGGTGGTG CCAATCCTGC CCTGGCACCA GTACTACAAG ATTTTGAAGC AACAATAGCT 
GAAAGAAGAA ATCTTGTTCT CAAAGAGAGC CAGAGAGCAA ACCAGGTCAC TTTTCTCTGC 
AGCAATATCA TTCATTTTGA AAGTCTACGA ACAAGAACTG CCGAAGCCTT AAGCCTGGAT 
GCTGCCTTAT TTGAACTCAT CAAACGGTGT CAGCAGATGT GTTCATTTGC ATCACAGTTC 
AACAGTTCAG TTTCTGAGTT AGAGCTTCGT TTATTACAGA GAGTGGACAC GACTCTTGAA 
CATCCTATTG GCAGTTCTGA GTGGCTTTTA TCAGCACATA AACAGTTGAC CCAGGATATG 
TCCACCCAAA GGGCTGTTCA GACAGAGAAG GAGCAGCAGA TCGAGACTGT TTGTGAGACC 
ATCCAGAGCC TGGTTGACAG CGTAAAGACT GTGCTCACGG GTCACAACCG GCAGCTTGGA 
GATGTAAAAC ATCTCCTGAA AGCCATGGCT AAGGACGAAG AGGCCGCTCT AGCAGATGCT 
GAAGATATCC CCTATGAGAG CAGTGTCAGG CAGTTCCTGG CCGAGTACAA ATCATGGCAA 
GACAACATTC AGACGGTTTT GTTCACACTG GTCCAGGCCA TGGGTCAGGT TCGAAGTCAA 
GAACATGTTG AAATGCTGCA GGAAATCACT CCCACCTTGA AAGAGCTGAA GACACAAAGC 
CAGAGTATCT ATAATAATTT AGTGAGTTTT GCATCACCCT TAGTCACCGA CGCAGCAAAC 
GAATGTTCAA GTCCAACATC ATCTGCTACT TACCAGCCGT CCTTTGCTGC AGCGGTTAGG 
AGTAACACTG GCCAGAAGAC TCAGCCTGAT GTCATGTCTC AGAATGCTAA AAAGCTGATT 
CAGAAAAATC TGGCTACATC AGCAGATACT CCACCAAGCA CCATCCCAGG AACTGGCAAG 
AGCATCGCCT GTAGTCCCAA AAAGGCTATC AGAGACCCTA AAACTGGGAA AGCTGTGCAA 
GAGAGAAACT CCTATGCAGT GAGTGTGTGG AAGAGAGTGA AAGCCAAGCT AGAAGGCCGA 
GATGTTGATC CAAACAGGAG GATGTCAGTT GCTGAGCAGG TTGACTATGT CATTAAGGAA 
GCAACTAATC TAGATAACTT GGCTCAGCTG TATGAAGGTT GGACAGCCTG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg1 ( XM_006223459.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006223521.1 Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X3, mRNA.

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CloneID	ORa14617
Clone ID Related Accession (Same CDS sequence)	XM_006223459.3
Accession Version	XM_006223459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase SMG1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006223459.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACTTTAAA ATACTCCTCA 
TCCAGAGATA GGGGTGTTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGGC CATGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGA TTAACAATGT GACCAGCCCT GAGTTTACCA GTGTTCAGCA TGGCAGTCGT 
GCGTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGCGAT CGATGTCGTA CTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ATGACAGAGA CCGAAGACTG 
GCTACTGTAA AGCAGCTGAA GGAGTTTATT CAGCAGCCAG AAAATAAACT GGTACTAGTT 
AAACAGTTGG ATAATATCTT GGCTGCTGTG CATGATGTAC TAAATGAAAG TAGTAAGTTG 
CTTCAGGAGT TGAGGCAAGA GGGAGCTTGC TGTCTTGGTC TTCTCTGTGC TTCTCTGAGC 
TACGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCGAAAGAT 
GAGGTGAAGC TCCTCTACCT GTGTGCCACT TACAGAGCAC TGGAGACTGT TGGCGAGAAG 
AAAGCCTTTT CTTCGGTGAT GCAGCTTGTG ATGACCAGCC TACAATCCAT TCTTGAAAAT 
GTGGATACAC CAGAATTACT TTGCAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGC 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAC 
ATAGATCATA CTCAGAAACC CTCCCTTACA CAGCAGGTGT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA CCTTGCATTT TCTACCACTC TTCTTGGTCA ATTTTTAGAA 
GACATGGAGG CATACGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAGTC AGTGGATGAA 
GATGTTCCTC CTCCATCAGT GTCATTACCA AAGCTGGCAG CGCTTCTCCG TGTGTTCAGT 
ACTGTGGTGA GGAGCATTGG GGAGCGCTTT AGCCCAATTC GGGGTCCACC AATTACCGAG 
GCATATGTAA CAGATGTTCT ATACAGAGTA ATGAGATGCG TGACGGCTGC AAACCAAGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT GCTCACATTG 
ATTGTTGAGC AGATAAATAC AAAACTCCCA TCATCATTTG TAGAAAAGCT TTTTATACCA 
TCATCTAAAC TACTATTTCT ACGATACCAC AAAGAAAAAG AGGTTGTTGC TGTAGCTCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AACATCCCCG TTCTGGAGAC TGCCTATAAA 
TTAATTTTGG GAGAAATGAC TTGTGCACTA AACAATTTGC TACACAGTCT CCAGCTTCCT 
GATGCCTGTT CTGAGATTAA ACATGAGGCT TTTCAGAATC ATGTGTTCAA CATTGACAAT 
GCCAACTTTG TAGTCATATT TGATCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCATTAATTG GGATGTGGGC ACTGTCTCCA ACTGTATTTG CACTGCTGAG TAAGAATCTG 
ATGATAGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC GGTGCTCTAC 
ACCCTATATT CCCATTGTAC CAGACACGAT CACTTCATTT CCAGTAGTCT TAGTTCTTCA 
TCCCCTTCCT TATTTGATGG CGCGGTGATT AGTACTGTAA CTACAGCCAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TTTGGGAATA TTACTAAAGA AAGATAATCT TAACCAGGAC 
ACAAGGAAAC TGTTAATGAC ATGGGCTTTG GAAGTAGCTG TATTAATGAA GAAGTCTGAA 
ACATATGCAC CTTTATTCTC CCTTCCATCT TTTCATAAAT TCTCCAAAGG CCTTTTAGCT 
AACACTCTTG TTGAAGATGT GAATATCTGC TTGCAAGCAT GTAGCAGTCT CCATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTGCTACAG AGGTGTGTTG ATGTCTGCCG TGTTCAGCTT 
GTCCATAGTG GAACTCGTAT TCGACAGGCC TTTGGAAAGC TGTTGAAGTC AATTCCTTTA 
GATGTTGTAC TAAGCAATAA CAATCACACA GAGATTCAGG AGATTTCTTT AGCATTGAGA 
AGCCACATGA GTAAAGCACC AAGTAACACG TTCCACCCTC AGGACTTTTC TGATGTCATC 
AGTTTCATTT TGTATGGAAA CTCTCACCGA ACAGGGAAGG ACAATTGGTT AGAAAGATTG 
TTCTATAGCT GCCAGAGGCT GGATAAGCGT GACCAATCCA CAATTCCCCG CAATCTTCTA 
AAGACAGATG CTGTCCTGTG GCAGTGGGCT ATTTGGGAAG CTGCACAGTT CACTGTTCTT 
TCAAAGTTGC GAACCCCACT GGGCAGAGCT CAGGACACGT TCCAGACAAT TGAAGGTATC 
ATTAGAAGTC TTGCAGCCCA CACACTAAAC CCTGACCAGG ATGTTAGTCA GTGGACCACT 
GCAGATAATG ATGAAGGCCA TGGGAGCAAT CAGCTTAGAC TTGTTCTTCT GCTTCAGTAT 
CTGGAGAACC TGGAGAAACT AATGTATAAC GCCTATGAGG GGTGTGCCAA TGCATTAACA 
TCACCTCCCA AGGTCATTAG GACTTTTTTC TATACCAATC GACAGACTTG CCAAGACTGG 
CTCACTCGGA TCCGTCTTTC CATCATGAGG GTCGGATTGT TGGCAGGTCA GCCTGCAGTG 
ACAGTGAGGC ACGGCTTTGA CTTACTTACT GAAATGAAAA CAAATAGTCT GTCTCAGGGG 
AATGAATTGG AAGTGACTAT CATGATGGTG GTAGAAGCGC TGTGTGAGCT CCACTGTCCT 
GAAGCTATAC AGGGGATTGC TGTCTGGTCA TCTTCAGCTG TTGGTAAAAA CCTTCTCTGG 
ATTAACTCAG TGGCCCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAGCACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAGTCTGTT 
CTCACATTGG CCAATGCTGG ACGTAACAGT GCCAGCCCCA AACACTCTCT AAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAATCAATC GACTCTTCCC CTGAAGTTAT CAACTACCTA 
GGAAATAAGG CATGTGAGTG TTACATCTCC ATTGCTGACT GGGCTGCTGT GCAGGAGTGG 
CAGAACGCGG TCCACGACCT GAAGAAGAAT GCCAACAGCA CCTCACTCAA TCTGAAAGCC 
GATTTCAACT ACATCAAGTC ACTAAGCAGC TTCGAGTCTG GAGAGTTTGT TGAATGCACT 
GAGCAGTTGG AATTATTGCC AGGAGAAAAT ATTAATCTGC TTGCTGGAGG GTCAAAAGAA 
AAAATAGATA TGAAAAAGCT GCTTCCTAAC ATGCTAAGCC CAGACCCACG GGAGCTTCAG 
AAATCCATTG AAGTCCAATT GTTGAGAAGC TCCGTGTTCC TGGCAACTGC TCTAAACCAT 
GTGGAACAAG ATCAGAAATG GCAGTCCTTA ACCGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACCTCACGCA TCGCTATTGG ACCACTAAGA TTGTCTACGC TAACGGTGTC ACAGTCCTTG 
CCAGTACTGA GTACCCTGCA GCTCTACTGC TCCTCTGCAC TGGAGAACAC AGTTTCTAAT 
AGACTTTCAA CAGAGGACTG TCTTATCCCA CTCTTTAGTG ATGCTCTTCG CTCATGTAAA 
CAGCATGATG TGCGGCCTTG GATGCAGGCG TTGAGATATA CCATGTACCA GAATCAACTG 
TTAGAGAAAA TTAAAGAACA AACAGTACCA ATTAGAAGCC ATCTCATGGA ACTAGGTCTG 
ACAGCTGCAA AATTTGCTAG AAAACGGGGG AATGTGTCAC TGGCAACAAG ACTGTTGGCA 
CAGTGCAGTG AGGTTCAGCT GGGAAAGACA ACCACTGCCC AGGACTTAGT CCAGCATTTT 
AAAAAACTGT CTACTCAAGG TCAAGTGGAT GAAAAATGGG GGCCTGAGCT TGATATTGAA 
AAAACCAAAT TGTTATACAC AGCAGGCCAG TCAACACACG CAATGGAAAT GTTGAGTTCT 
TGTGCTATAT CTTTCTGCAA ATCTGCGAAA GCTGAATATG CTGTGGCTAA GTCAATTCTG 
ACCCTGGCTA AATGGGTCCA GGCAGAATGG AAAGAGATTT CGGGACAGCT GAGGCAGGTG 
TACCGAGCTC AGCAGCAGCA GAACCTTTCA GGCCTTTCTA CTTTGTCTAG GAATATACTC 
GCTCTTATAG AGCTACCGTC TGCTAATACA GTGGGAGAAG AGCACCCTCG GATTGAGAGT 
GAGTCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TGCAGGCACC TGAAGTAGCC AAATCTTGGG CAGCATTGGC TAGTTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGTTG 
CCTAGAGAAA AATCTGAAGT TCAAAATCTG CTTCCAGATA CTATAACTGA AGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAAGCA GTATGTCGGC CAGCAGGAAT TCAGGATGAA 
GATATAACAC TACAGATAAC AGAGAGTGAA GATAATGAGG ATGATGACAT GGTCGATGTT 
ATCTGGCGGC AGTTAATATC AAGCTGTCCC TGGCTTTCTG AACTGGATGA AAATGCAACG 
GAAGGTGTTA TTAAAGTGTG GAGGAAAGTT GTGGACAGAA TCTTCAGCCT GTACAAATTG 
TCTTGCAGTG CATATTTTAC TTTCCTTAAG CTGAATGCTG GTCAGGTTCT GTTAGATGAG 
GATGACCCCA GGCTCCATTT AAGTCACAGA GCTGAGCAGA GCACTGATGA TGTGATTGTG 
ATGGCTACCC TGCGACTGCT CAGATTGCTA GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAACATG GCTTGGAGAC AACCCCTACT GCTCCATGGA GAGGAATTAT TCCACAGCTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTACGCCAGA GTATTTGTAA CCTCCTTTGC 
CGTGTAGCCC AAGATTCCCC TCACCTCATA TTGTATCCTG CAATAGTAGG AACCATATCA 
CTTAGCAGTG AATCCCAGGC TTCAGGAAAT AAGTATTCCT CTGCAATCCC AACTTTACTT 
GGAAATATTC AAGGAGAAGA ACTGCTGGTT TCTGAATGTG AAGGAGGAAG TCCTCCTGCT 
TCTCAGGATA GCAACAAAGA TGAACCTAAA AGTGGTTTAA ATGAAGATCA GGCCATGATG 
CAGGATTGCT ACAGCAAAAT TGTAGATAAA CTATCCTCTG CAAATCCAAC GATGGTTTTA 
CAGGTCCAGA TGCTTGTGGC AGAGCTACGT AGGGTCACTG TACTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGTTACAGCA GCATATGTAC GTCCTGAGGC GAATTCAGCA GCTGGAGGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTGCGCAAAG AAGAGAAAAT TGCCATCATG 
CGAGAGAAGC ACACAGCACT GATGAAGCCC ATCGTGTTTG CTTTGGAGCA CGTGCGGAGC 
ATCACTGCTG CCCCTGCAGA GACCCCTCAT GAAAAGTGGT TTCAGGATAA CTATGGTGAT 
GCCATTGACA ATGCCCTTGA AAAACTAAAG ACCCCATCCA ACCCTGCCAA GCCTGGAAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTG AGTTTGCAAC AGAGAGCGCA GAAACGAGCA 
AGCTACATCT TGCGACTTGA TGAGATCAGT CCTTGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCACTCC CTGGAGAAGT CTCTGCCAGA GACACTGTGA CAATTCACAG TGTGGGTGGA 
ACTATCACTA TCTTACCAAC TAAAACCAAG CCCAAGAAAC TCCTCTTTCT TGGATCAGAT 
GGAAAGAGCT ATCCTTACCT TTTCAAAGGT CTAGAAGATT TGCATCTGGA TGAGAGAATC 
ATGCAGTTTT TATCTATTGT GAATACTATG TTTGCTACAA TTAATCGGCA GGAAACACCC 
CGATTCCATG CACGGCACTA TTCTGTAACA CCACTGGGGA CGAGATCGGG GCTGATCCAG 
TGGGTGGATG GAGCCACACC ATTGTTCGGG CTTTACAAAC GATGGCAACA GCGGGAGGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAGACTC CTCAGAATCC CAGCATTGTA 
CCGCGTCCTA GTGAACTTTA CTATAGTAAA ATTGGTCCTG CTTTGAAAAC AGTTGGCCTT 
AGCCTGGATG TGTCAAGGAG GGATTGGCCC CTCCATGTGA TGAAGGCAGT GTTGGAGGAG 
TTAATGGAGG CCACACCCCC AAACCTTCTG GCCAAAGAAC TCTGGTCATC TTGCACCACT 
CCCGATGAGT GGTGGAGAGT CACACAGTCC TATGCAAGAT CCACCGCTGT CATGTCTATG 
GTTGGCTACA TAATTGGCCT TGGAGACAGA CATCTGGATA ACGTCCTCAT AGATATGACA 
ACTGGAGAAG TAGTTCACAT AGATTATAAT GTTTGCTTTG AAAAAGGTAA AAGCCTTAGA 
GTTCCTGAGA AAGTGCCTTT TCGAATGACA CAAAACATTG AAACAGCACT GGGTGTAACT 
GGAGTAGAAG GTGTTTTTAG GCTTTCCTGT GAGCAGGTTC TCCACATCAT GCGACGAGGC 
CGAGAGACTC TGCTGACACT CTTGGAGGCT TTTGTGTATG ACCCACTGGT GGACTGGACA 
GCCGGCGGTG AGGCTGGGTT TGCAGGTGCT GTCTATGGTG GAGGTGGCCA ACAGGCCGAG 
AGCAAGCAGA GTAAGCGAGA GATGGAGCGT GAGATTACCC GCAGCCTCTT TTCTTCCAGA 
GTTGCTGAGA TTAAGGTGAA CTGGTTTAAG AACCGGGATG AGATGCTAGT TGTGCTTCCC 
AAGCTGGACA GCAGCTTAGA TGAATACCTA AGCCTGCAGG AGCAGCTGAC AGATGTTGAA 
AAGCTGCAGG GCAAACTGCT GGAGGAAATA GAGTTTCTAG AAGGAGCTGA AGGAGTGGAT 
CATCCATCTC GCACTCTGCA ACACAGATAT TCTGAGCATA CTCAACTACA GACCCAGCAA 
AGAGCTGTTC AGGAAGCAAT CCAGGTGAAG CTGAATGAGT TTGAACAATG GATAACACAT 
TATCAGGCTG CATTCAATAA CTTAGAAGCA ACTCAGCTTG CAAGTTTGCT TCAAGAGATA 
AGCACACAAA TGGACCTTGG TCCCCCAAGC TATGTTCCAG CAACAGCTTT TCTACAGAAT 
GCTGGCCAAG CACACCTCAT CAGCCAGTGT GAGCAGCTAG AGGGGGAAGT TGGTACTCTG 
CTGCAGCAGA GGCGCTCTGT GCTCCGTGGC TGTCTGGAAC AGCTGCATCA CTATGCAACT 
GTCGCTCTGC AGTATCCGAA AGCAATATTC CAGAAACATC GAATTGAACA GTGGAAGGCC 
TGGATGGAAG AGCTCATCTG CAACACCACA GTAGAACGCT GCCAAGAACT CTATAGGAAA 
TATGAGATGC AGTATGCTCC TCAGCCCCCT CCAACAGTCT GTCAGTTCAT TACTGCCACT 
GAAATGACCC TTCAGCGGTA TGCAGCAGAC ATAAACAGCA GACTTATTAG ACAAGTGGAA 
CGCTTGAAGC AAGAAGCTGT CACTGTGCCA GTTTGTGAGG ATCAGTTGAA AGAAATTGAG 
CGTTGCATTA AAGTTTTTCT TCATGAGAAT GGAGAAGAAG GATCTCTGAG TCTAGCAAGT 
GTCATTATTT CTGCCCTTTG TACTCTTACA AGGCGCAACC TTATGATGGA AGGTGCAGCT 
TCAAGTGCTG GAGAGCAGCT GGTTGATCTG ACATCCAGAG ATGGAGCCTG GTTCCTAGAG 
GAGCTGTGTA GTATGAGTGG AAATGTCACC TGCTTGGTGC AGTTACTGAA GCAGTGCCAC 
CTGGTACCAC AGGACTTGGA TATTCCCAAC CCAGTGGAAG CATCTGAGGC TGTTCATTTA 
GCCAATGGAG TGTATACATC TCTGCAGGAA TTAAATTCAA ATTTCCGGCA AATCATATTC 
CCAGAAGCTC TTAGATGTTT AATGAAGGGG GAGTGCACTT TAGAAAGTAT GCTACATGAA 
CTAGACAGTC TCATCGAGCA GACTACTGAT GGAGTTCCTC TGCAGACTCT GGTGGAGTCG 
CTTCAAGCCT ACTTAAGAAA CACAGCCATG GGACTTGAAG AAGAAACTCA TGCTCATTAT 
ATTGATGTTG CCAGAATGCT ACATGCTCAA TATGGTGAAT TAATCCAACC AAGAAATGGT 
TCAGTTGATG AAACACCAAA AATGTCAGCT GGCCAGATGC TTTTGGTAGC ATTTGATGGC 
ATGTTTGCCC AAGTTGAAAC TGCTTTTGGC TTATTAGTTG AGAAGTTAAA CAAGATGGAG 
ATTCCCGTTG CTTGGAGGAA GATTGACATT ATCCGGGAAG CCAGGAGCAC CCAGGCCAAT 
TTTTTTGATG ATGACAATCA CCGGCAGGTG CTAGAAGAGA TTTTCTTTTT AAAAAGACTG 
CAGACAATTA AGGAGTTCTT CAGGCTCTGT GGTACCTTTT CTAAAACATT GTCAGGATCA 
AGTTCACTTG AAGATCAGAA TACTGTAAAT GGACCTGTTC AGATTGTCAA TGTGAAAACC 
CTTTTTAGAA ATTCTTGCTT TAGTGAAGAC CAAATGGCCA AGCCTATCAA GGCCTTTACA 
GCTGACTTTG TAAGACAGCT CTTGATAGGA CTCCCAAACC AAGCCCTCGG ACTCACATTG 
TGTAGCTTTA TCAGTGCCCT GGGCGTAGAC ATCATTGCTC AGGTAGAGGC AAAGGATTTC 
GGAGCTGAAA GCAAAGTCTC TGTGGACGAT CTGTGTAAGA AAGCTGTGGA GCACAACATC 
CAGGTTGGGA GATTCTCACA GTTGGTGATG AACAGAGCTA CCGTGTTAGC CAGCTCCTAT 
GATACAGCCT GGAAGAAGCA TGACCTGGTG CGCAGGCTGG AAACCAGCAT TTCTTCCTGT 
AAGACAAGCT TACAGCGGGT GCAACTGCAC ATTGCCATGT TCCAGTGGCA ACATGAAGAC 
CTCCTTATCA ACAGACCACA AGCCATGTCA GTCACCCCAC CTCGGTCTGC AATCCTGACC 
AGCATGAAGA AGAAACTGCA TGCTCTCAGT CAGATTGAGA CTTCAATCGG GACTGTTCAG 
GAGAAACTAG CAGCTCTTGA AGCAAGCATT GAGCAGCGGC TGAAGTGGGC AGGTGGTGCC 
AATCCTGCCC TGGCACCAGT ACTACAAGAT TTTGAAGCAA CAATAGCTGA AAGAAGAAAT 
CTTGTTCTCA AAGAGAGCCA GAGAGCAAAC CAGGTCACTT TTCTCTGCAG CAATATCATT 
CATTTTGAAA GTCTACGAAC AAGAACTGCC GAAGCCTTAA GCCTGGATGC TGCCTTATTT 
GAACTCATCA AACGGTGTCA GCAGATGTGT TCATTTGCAT CACAGTTCAA CAGTTCAGTT 
TCTGAGTTAG AGCTTCGTTT ATTACAGAGA GTGGACACGA CTCTTGAACA TCCTATTGGC 
AGTTCTGAGT GGCTTTTATC AGCACATAAA CAGTTGACCC AGGATATGTC CACCCAAAGG 
GCTGTTCAGA CAGAGAAGGA GCAGCAGATC GAGACTGTTT GTGAGACCAT CCAGAGCCTG 
GTTGACAGCG TAAAGACTGT GCTCACGGGT CACAACCGGC AGCTTGGAGA TGTAAAACAT 
CTCCTGAAAG CCATGGCTAA GGACGAAGAG GCCGCTCTAG CAGATGCTGA AGATATCCCC 
TATGAGAGCA GTGTCAGGCA GTTCCTGGCC GAGTACAAAT CATGGCAAGA CAACATTCAG 
ACGGTTTTGT TCACACTGGT CCAGGCCATG GGTCAGGTTC GAAGTCAAGA ACATGTTGAA 
ATGCTGCAGG AAATCACTCC CACCTTGAAA GAGCTGAAGA CACAAAGCCA GAGTATCTAT 
AATAATTTAG TGAGTTTTGC ATCACCCTTA GTCACCGACG CAGCAAACGA ATGTTCAAGT 
CCAACATCAT CTGCTACTTA CCAGCCGTCC TTTGCTGCAG CGGTTAGGAG TAACACTGGC 
CAGAAGACTC AGCCTGATGT CATGTCTCAG AATGCTAAAA AGCTGATTCA GAAAAATCTG 
GCTACATCAG CAGATACTCC ACCAAGCACC ATCCCAGGAA CTGGCAAGAG CATCGCCTGT 
AGTCCCAAAA AGGCTATCAG AGACCCTAAA ACTGGGAAAG CTGTGCAAGA GAGAAACTCC 
TATGCAGTGA GTGTGTGGAA GAGAGTGAAA GCCAAGCTAG AAGGCCGAGA TGTTGATCCA 
AACAGGAGGA TGTCAGTTGC TGAGCAGGTT GACTATGTCA TTAAGGAAGC AACTAATCTA 
GATAACTTGG CTCAGCTGTA TGAAGGTTGG ACAGCCTGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006223521.1
CDS	130..11034
Translation	MRFLRTDSASADPDTLKYSSSRDRGVSSSYGLQPSNSAVVSRQRHDDTRGHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRINNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYRALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFQNHVFNIDNANFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFSKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSAVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKSIDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKKNANSTSLNLKADFNYIKSLSSFESGEFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVFLATALNHVEQDQKWQSLTENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSDALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWVQAEWKEISGQLRQVYRAQQQQNLSGLSTLSRNILALIELPSANTVGEEHPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEDDDMVDVIWRQLISSCPWLSELDENATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVLLDEDDPRLHLSHRAEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKYSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIDNALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLDEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPSIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSRTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGTLLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKAWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPVEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGECTLESMLHELDSLIEQTTDGVPLQTLVESLQAYLRNTAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPVAWRKIDIIREARSTQANFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQVGRFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPRSAILTSMKKKLHALSQIETSIGTVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRANQVTFLCSNIIHFESLRTRTAEALSLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAVQTEKEQQIETVCETIQSLVDSVKTVLTGHNRQLGDVKHLLKAMAKDEEAALADAEDIPYESSVRQFLAEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDAANECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNAKKLIQKNLATSADTPPSTIPGTGKSIACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_006223459.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006223459.3

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACTTTAAA ATACTCCTCA 
TCCAGAGATA GGGGTGTTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGGC CATGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGA TTAACAATGT GACCAGCCCT GAGTTTACCA GTGTTCAGCA TGGCAGTCGT 
GCGTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGCGAT CGATGTCGTA CTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ATGACAGAGA CCGAAGACTG 
GCTACTGTAA AGCAGCTGAA GGAGTTTATT CAGCAGCCAG AAAATAAACT GGTACTAGTT 
AAACAGTTGG ATAATATCTT GGCTGCTGTG CATGATGTAC TAAATGAAAG TAGTAAGTTG 
CTTCAGGAGT TGAGGCAAGA GGGAGCTTGC TGTCTTGGTC TTCTCTGTGC TTCTCTGAGC 
TACGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCGAAAGAT 
GAGGTGAAGC TCCTCTACCT GTGTGCCACT TACAGAGCAC TGGAGACTGT TGGCGAGAAG 
AAAGCCTTTT CTTCGGTGAT GCAGCTTGTG ATGACCAGCC TACAATCCAT TCTTGAAAAT 
GTGGATACAC CAGAATTACT TTGCAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGC 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAC 
ATAGATCATA CTCAGAAACC CTCCCTTACA CAGCAGGTGT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA CCTTGCATTT TCTACCACTC TTCTTGGTCA ATTTTTAGAA 
GACATGGAGG CATACGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAGTC AGTGGATGAA 
GATGTTCCTC CTCCATCAGT GTCATTACCA AAGCTGGCAG CGCTTCTCCG TGTGTTCAGT 
ACTGTGGTGA GGAGCATTGG GGAGCGCTTT AGCCCAATTC GGGGTCCACC AATTACCGAG 
GCATATGTAA CAGATGTTCT ATACAGAGTA ATGAGATGCG TGACGGCTGC AAACCAAGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT GCTCACATTG 
ATTGTTGAGC AGATAAATAC AAAACTCCCA TCATCATTTG TAGAAAAGCT TTTTATACCA 
TCATCTAAAC TACTATTTCT ACGATACCAC AAAGAAAAAG AGGTTGTTGC TGTAGCTCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AACATCCCCG TTCTGGAGAC TGCCTATAAA 
TTAATTTTGG GAGAAATGAC TTGTGCACTA AACAATTTGC TACACAGTCT CCAGCTTCCT 
GATGCCTGTT CTGAGATTAA ACATGAGGCT TTTCAGAATC ATGTGTTCAA CATTGACAAT 
GCCAACTTTG TAGTCATATT TGATCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCATTAATTG GGATGTGGGC ACTGTCTCCA ACTGTATTTG CACTGCTGAG TAAGAATCTG 
ATGATAGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC GGTGCTCTAC 
ACCCTATATT CCCATTGTAC CAGACACGAT CACTTCATTT CCAGTAGTCT TAGTTCTTCA 
TCCCCTTCCT TATTTGATGG CGCGGTGATT AGTACTGTAA CTACAGCCAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TTTGGGAATA TTACTAAAGA AAGATAATCT TAACCAGGAC 
ACAAGGAAAC TGTTAATGAC ATGGGCTTTG GAAGTAGCTG TATTAATGAA GAAGTCTGAA 
ACATATGCAC CTTTATTCTC CCTTCCATCT TTTCATAAAT TCTCCAAAGG CCTTTTAGCT 
AACACTCTTG TTGAAGATGT GAATATCTGC TTGCAAGCAT GTAGCAGTCT CCATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTGCTACAG AGGTGTGTTG ATGTCTGCCG TGTTCAGCTT 
GTCCATAGTG GAACTCGTAT TCGACAGGCC TTTGGAAAGC TGTTGAAGTC AATTCCTTTA 
GATGTTGTAC TAAGCAATAA CAATCACACA GAGATTCAGG AGATTTCTTT AGCATTGAGA 
AGCCACATGA GTAAAGCACC AAGTAACACG TTCCACCCTC AGGACTTTTC TGATGTCATC 
AGTTTCATTT TGTATGGAAA CTCTCACCGA ACAGGGAAGG ACAATTGGTT AGAAAGATTG 
TTCTATAGCT GCCAGAGGCT GGATAAGCGT GACCAATCCA CAATTCCCCG CAATCTTCTA 
AAGACAGATG CTGTCCTGTG GCAGTGGGCT ATTTGGGAAG CTGCACAGTT CACTGTTCTT 
TCAAAGTTGC GAACCCCACT GGGCAGAGCT CAGGACACGT TCCAGACAAT TGAAGGTATC 
ATTAGAAGTC TTGCAGCCCA CACACTAAAC CCTGACCAGG ATGTTAGTCA GTGGACCACT 
GCAGATAATG ATGAAGGCCA TGGGAGCAAT CAGCTTAGAC TTGTTCTTCT GCTTCAGTAT 
CTGGAGAACC TGGAGAAACT AATGTATAAC GCCTATGAGG GGTGTGCCAA TGCATTAACA 
TCACCTCCCA AGGTCATTAG GACTTTTTTC TATACCAATC GACAGACTTG CCAAGACTGG 
CTCACTCGGA TCCGTCTTTC CATCATGAGG GTCGGATTGT TGGCAGGTCA GCCTGCAGTG 
ACAGTGAGGC ACGGCTTTGA CTTACTTACT GAAATGAAAA CAAATAGTCT GTCTCAGGGG 
AATGAATTGG AAGTGACTAT CATGATGGTG GTAGAAGCGC TGTGTGAGCT CCACTGTCCT 
GAAGCTATAC AGGGGATTGC TGTCTGGTCA TCTTCAGCTG TTGGTAAAAA CCTTCTCTGG 
ATTAACTCAG TGGCCCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAGCACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAGTCTGTT 
CTCACATTGG CCAATGCTGG ACGTAACAGT GCCAGCCCCA AACACTCTCT AAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAATCAATC GACTCTTCCC CTGAAGTTAT CAACTACCTA 
GGAAATAAGG CATGTGAGTG TTACATCTCC ATTGCTGACT GGGCTGCTGT GCAGGAGTGG 
CAGAACGCGG TCCACGACCT GAAGAAGAAT GCCAACAGCA CCTCACTCAA TCTGAAAGCC 
GATTTCAACT ACATCAAGTC ACTAAGCAGC TTCGAGTCTG GAGAGTTTGT TGAATGCACT 
GAGCAGTTGG AATTATTGCC AGGAGAAAAT ATTAATCTGC TTGCTGGAGG GTCAAAAGAA 
AAAATAGATA TGAAAAAGCT GCTTCCTAAC ATGCTAAGCC CAGACCCACG GGAGCTTCAG 
AAATCCATTG AAGTCCAATT GTTGAGAAGC TCCGTGTTCC TGGCAACTGC TCTAAACCAT 
GTGGAACAAG ATCAGAAATG GCAGTCCTTA ACCGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACCTCACGCA TCGCTATTGG ACCACTAAGA TTGTCTACGC TAACGGTGTC ACAGTCCTTG 
CCAGTACTGA GTACCCTGCA GCTCTACTGC TCCTCTGCAC TGGAGAACAC AGTTTCTAAT 
AGACTTTCAA CAGAGGACTG TCTTATCCCA CTCTTTAGTG ATGCTCTTCG CTCATGTAAA 
CAGCATGATG TGCGGCCTTG GATGCAGGCG TTGAGATATA CCATGTACCA GAATCAACTG 
TTAGAGAAAA TTAAAGAACA AACAGTACCA ATTAGAAGCC ATCTCATGGA ACTAGGTCTG 
ACAGCTGCAA AATTTGCTAG AAAACGGGGG AATGTGTCAC TGGCAACAAG ACTGTTGGCA 
CAGTGCAGTG AGGTTCAGCT GGGAAAGACA ACCACTGCCC AGGACTTAGT CCAGCATTTT 
AAAAAACTGT CTACTCAAGG TCAAGTGGAT GAAAAATGGG GGCCTGAGCT TGATATTGAA 
AAAACCAAAT TGTTATACAC AGCAGGCCAG TCAACACACG CAATGGAAAT GTTGAGTTCT 
TGTGCTATAT CTTTCTGCAA ATCTGCGAAA GCTGAATATG CTGTGGCTAA GTCAATTCTG 
ACCCTGGCTA AATGGGTCCA GGCAGAATGG AAAGAGATTT CGGGACAGCT GAGGCAGGTG 
TACCGAGCTC AGCAGCAGCA GAACCTTTCA GGCCTTTCTA CTTTGTCTAG GAATATACTC 
GCTCTTATAG AGCTACCGTC TGCTAATACA GTGGGAGAAG AGCACCCTCG GATTGAGAGT 
GAGTCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TGCAGGCACC TGAAGTAGCC AAATCTTGGG CAGCATTGGC TAGTTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGTTG 
CCTAGAGAAA AATCTGAAGT TCAAAATCTG CTTCCAGATA CTATAACTGA AGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAAGCA GTATGTCGGC CAGCAGGAAT TCAGGATGAA 
GATATAACAC TACAGATAAC AGAGAGTGAA GATAATGAGG ATGATGACAT GGTCGATGTT 
ATCTGGCGGC AGTTAATATC AAGCTGTCCC TGGCTTTCTG AACTGGATGA AAATGCAACG 
GAAGGTGTTA TTAAAGTGTG GAGGAAAGTT GTGGACAGAA TCTTCAGCCT GTACAAATTG 
TCTTGCAGTG CATATTTTAC TTTCCTTAAG CTGAATGCTG GTCAGGTTCT GTTAGATGAG 
GATGACCCCA GGCTCCATTT AAGTCACAGA GCTGAGCAGA GCACTGATGA TGTGATTGTG 
ATGGCTACCC TGCGACTGCT CAGATTGCTA GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAACATG GCTTGGAGAC AACCCCTACT GCTCCATGGA GAGGAATTAT TCCACAGCTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTACGCCAGA GTATTTGTAA CCTCCTTTGC 
CGTGTAGCCC AAGATTCCCC TCACCTCATA TTGTATCCTG CAATAGTAGG AACCATATCA 
CTTAGCAGTG AATCCCAGGC TTCAGGAAAT AAGTATTCCT CTGCAATCCC AACTTTACTT 
GGAAATATTC AAGGAGAAGA ACTGCTGGTT TCTGAATGTG AAGGAGGAAG TCCTCCTGCT 
TCTCAGGATA GCAACAAAGA TGAACCTAAA AGTGGTTTAA ATGAAGATCA GGCCATGATG 
CAGGATTGCT ACAGCAAAAT TGTAGATAAA CTATCCTCTG CAAATCCAAC GATGGTTTTA 
CAGGTCCAGA TGCTTGTGGC AGAGCTACGT AGGGTCACTG TACTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGTTACAGCA GCATATGTAC GTCCTGAGGC GAATTCAGCA GCTGGAGGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTGCGCAAAG AAGAGAAAAT TGCCATCATG 
CGAGAGAAGC ACACAGCACT GATGAAGCCC ATCGTGTTTG CTTTGGAGCA CGTGCGGAGC 
ATCACTGCTG CCCCTGCAGA GACCCCTCAT GAAAAGTGGT TTCAGGATAA CTATGGTGAT 
GCCATTGACA ATGCCCTTGA AAAACTAAAG ACCCCATCCA ACCCTGCCAA GCCTGGAAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTG AGTTTGCAAC AGAGAGCGCA GAAACGAGCA 
AGCTACATCT TGCGACTTGA TGAGATCAGT CCTTGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCACTCC CTGGAGAAGT CTCTGCCAGA GACACTGTGA CAATTCACAG TGTGGGTGGA 
ACTATCACTA TCTTACCAAC TAAAACCAAG CCCAAGAAAC TCCTCTTTCT TGGATCAGAT 
GGAAAGAGCT ATCCTTACCT TTTCAAAGGT CTAGAAGATT TGCATCTGGA TGAGAGAATC 
ATGCAGTTTT TATCTATTGT GAATACTATG TTTGCTACAA TTAATCGGCA GGAAACACCC 
CGATTCCATG CACGGCACTA TTCTGTAACA CCACTGGGGA CGAGATCGGG GCTGATCCAG 
TGGGTGGATG GAGCCACACC ATTGTTCGGG CTTTACAAAC GATGGCAACA GCGGGAGGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAGACTC CTCAGAATCC CAGCATTGTA 
CCGCGTCCTA GTGAACTTTA CTATAGTAAA ATTGGTCCTG CTTTGAAAAC AGTTGGCCTT 
AGCCTGGATG TGTCAAGGAG GGATTGGCCC CTCCATGTGA TGAAGGCAGT GTTGGAGGAG 
TTAATGGAGG CCACACCCCC AAACCTTCTG GCCAAAGAAC TCTGGTCATC TTGCACCACT 
CCCGATGAGT GGTGGAGAGT CACACAGTCC TATGCAAGAT CCACCGCTGT CATGTCTATG 
GTTGGCTACA TAATTGGCCT TGGAGACAGA CATCTGGATA ACGTCCTCAT AGATATGACA 
ACTGGAGAAG TAGTTCACAT AGATTATAAT GTTTGCTTTG AAAAAGGTAA AAGCCTTAGA 
GTTCCTGAGA AAGTGCCTTT TCGAATGACA CAAAACATTG AAACAGCACT GGGTGTAACT 
GGAGTAGAAG GTGTTTTTAG GCTTTCCTGT GAGCAGGTTC TCCACATCAT GCGACGAGGC 
CGAGAGACTC TGCTGACACT CTTGGAGGCT TTTGTGTATG ACCCACTGGT GGACTGGACA 
GCCGGCGGTG AGGCTGGGTT TGCAGGTGCT GTCTATGGTG GAGGTGGCCA ACAGGCCGAG 
AGCAAGCAGA GTAAGCGAGA GATGGAGCGT GAGATTACCC GCAGCCTCTT TTCTTCCAGA 
GTTGCTGAGA TTAAGGTGAA CTGGTTTAAG AACCGGGATG AGATGCTAGT TGTGCTTCCC 
AAGCTGGACA GCAGCTTAGA TGAATACCTA AGCCTGCAGG AGCAGCTGAC AGATGTTGAA 
AAGCTGCAGG GCAAACTGCT GGAGGAAATA GAGTTTCTAG AAGGAGCTGA AGGAGTGGAT 
CATCCATCTC GCACTCTGCA ACACAGATAT TCTGAGCATA CTCAACTACA GACCCAGCAA 
AGAGCTGTTC AGGAAGCAAT CCAGGTGAAG CTGAATGAGT TTGAACAATG GATAACACAT 
TATCAGGCTG CATTCAATAA CTTAGAAGCA ACTCAGCTTG CAAGTTTGCT TCAAGAGATA 
AGCACACAAA TGGACCTTGG TCCCCCAAGC TATGTTCCAG CAACAGCTTT TCTACAGAAT 
GCTGGCCAAG CACACCTCAT CAGCCAGTGT GAGCAGCTAG AGGGGGAAGT TGGTACTCTG 
CTGCAGCAGA GGCGCTCTGT GCTCCGTGGC TGTCTGGAAC AGCTGCATCA CTATGCAACT 
GTCGCTCTGC AGTATCCGAA AGCAATATTC CAGAAACATC GAATTGAACA GTGGAAGGCC 
TGGATGGAAG AGCTCATCTG CAACACCACA GTAGAACGCT GCCAAGAACT CTATAGGAAA 
TATGAGATGC AGTATGCTCC TCAGCCCCCT CCAACAGTCT GTCAGTTCAT TACTGCCACT 
GAAATGACCC TTCAGCGGTA TGCAGCAGAC ATAAACAGCA GACTTATTAG ACAAGTGGAA 
CGCTTGAAGC AAGAAGCTGT CACTGTGCCA GTTTGTGAGG ATCAGTTGAA AGAAATTGAG 
CGTTGCATTA AAGTTTTTCT TCATGAGAAT GGAGAAGAAG GATCTCTGAG TCTAGCAAGT 
GTCATTATTT CTGCCCTTTG TACTCTTACA AGGCGCAACC TTATGATGGA AGGTGCAGCT 
TCAAGTGCTG GAGAGCAGCT GGTTGATCTG ACATCCAGAG ATGGAGCCTG GTTCCTAGAG 
GAGCTGTGTA GTATGAGTGG AAATGTCACC TGCTTGGTGC AGTTACTGAA GCAGTGCCAC 
CTGGTACCAC AGGACTTGGA TATTCCCAAC CCAGTGGAAG CATCTGAGGC TGTTCATTTA 
GCCAATGGAG TGTATACATC TCTGCAGGAA TTAAATTCAA ATTTCCGGCA AATCATATTC 
CCAGAAGCTC TTAGATGTTT AATGAAGGGG GAGTGCACTT TAGAAAGTAT GCTACATGAA 
CTAGACAGTC TCATCGAGCA GACTACTGAT GGAGTTCCTC TGCAGACTCT GGTGGAGTCG 
CTTCAAGCCT ACTTAAGAAA CACAGCCATG GGACTTGAAG AAGAAACTCA TGCTCATTAT 
ATTGATGTTG CCAGAATGCT ACATGCTCAA TATGGTGAAT TAATCCAACC AAGAAATGGT 
TCAGTTGATG AAACACCAAA AATGTCAGCT GGCCAGATGC TTTTGGTAGC ATTTGATGGC 
ATGTTTGCCC AAGTTGAAAC TGCTTTTGGC TTATTAGTTG AGAAGTTAAA CAAGATGGAG 
ATTCCCGTTG CTTGGAGGAA GATTGACATT ATCCGGGAAG CCAGGAGCAC CCAGGCCAAT 
TTTTTTGATG ATGACAATCA CCGGCAGGTG CTAGAAGAGA TTTTCTTTTT AAAAAGACTG 
CAGACAATTA AGGAGTTCTT CAGGCTCTGT GGTACCTTTT CTAAAACATT GTCAGGATCA 
AGTTCACTTG AAGATCAGAA TACTGTAAAT GGACCTGTTC AGATTGTCAA TGTGAAAACC 
CTTTTTAGAA ATTCTTGCTT TAGTGAAGAC CAAATGGCCA AGCCTATCAA GGCCTTTACA 
GCTGACTTTG TAAGACAGCT CTTGATAGGA CTCCCAAACC AAGCCCTCGG ACTCACATTG 
TGTAGCTTTA TCAGTGCCCT GGGCGTAGAC ATCATTGCTC AGGTAGAGGC AAAGGATTTC 
GGAGCTGAAA GCAAAGTCTC TGTGGACGAT CTGTGTAAGA AAGCTGTGGA GCACAACATC 
CAGGTTGGGA GATTCTCACA GTTGGTGATG AACAGAGCTA CCGTGTTAGC CAGCTCCTAT 
GATACAGCCT GGAAGAAGCA TGACCTGGTG CGCAGGCTGG AAACCAGCAT TTCTTCCTGT 
AAGACAAGCT TACAGCGGGT GCAACTGCAC ATTGCCATGT TCCAGTGGCA ACATGAAGAC 
CTCCTTATCA ACAGACCACA AGCCATGTCA GTCACCCCAC CTCGGTCTGC AATCCTGACC 
AGCATGAAGA AGAAACTGCA TGCTCTCAGT CAGATTGAGA CTTCAATCGG GACTGTTCAG 
GAGAAACTAG CAGCTCTTGA AGCAAGCATT GAGCAGCGGC TGAAGTGGGC AGGTGGTGCC 
AATCCTGCCC TGGCACCAGT ACTACAAGAT TTTGAAGCAA CAATAGCTGA AAGAAGAAAT 
CTTGTTCTCA AAGAGAGCCA GAGAGCAAAC CAGGTCACTT TTCTCTGCAG CAATATCATT 
CATTTTGAAA GTCTACGAAC AAGAACTGCC GAAGCCTTAA GCCTGGATGC TGCCTTATTT 
GAACTCATCA AACGGTGTCA GCAGATGTGT TCATTTGCAT CACAGTTCAA CAGTTCAGTT 
TCTGAGTTAG AGCTTCGTTT ATTACAGAGA GTGGACACGA CTCTTGAACA TCCTATTGGC 
AGTTCTGAGT GGCTTTTATC AGCACATAAA CAGTTGACCC AGGATATGTC CACCCAAAGG 
GCTGTTCAGA CAGAGAAGGA GCAGCAGATC GAGACTGTTT GTGAGACCAT CCAGAGCCTG 
GTTGACAGCG TAAAGACTGT GCTCACGGGT CACAACCGGC AGCTTGGAGA TGTAAAACAT 
CTCCTGAAAG CCATGGCTAA GGACGAAGAG GCCGCTCTAG CAGATGCTGA AGATATCCCC 
TATGAGAGCA GTGTCAGGCA GTTCCTGGCC GAGTACAAAT CATGGCAAGA CAACATTCAG 
ACGGTTTTGT TCACACTGGT CCAGGCCATG GGTCAGGTTC GAAGTCAAGA ACATGTTGAA 
ATGCTGCAGG AAATCACTCC CACCTTGAAA GAGCTGAAGA CACAAAGCCA GAGTATCTAT 
AATAATTTAG TGAGTTTTGC ATCACCCTTA GTCACCGACG CAGCAAACGA ATGTTCAAGT 
CCAACATCAT CTGCTACTTA CCAGCCGTCC TTTGCTGCAG CGGTTAGGAG TAACACTGGC 
CAGAAGACTC AGCCTGATGT CATGTCTCAG AATGCTAAAA AGCTGATTCA GAAAAATCTG 
GCTACATCAG CAGATACTCC ACCAAGCACC ATCCCAGGAA CTGGCAAGAG CATCGCCTGT 
AGTCCCAAAA AGGCTATCAG AGACCCTAAA ACTGGGAAAG CTGTGCAAGA GAGAAACTCC 
TATGCAGTGA GTGTGTGGAA GAGAGTGAAA GCCAAGCTAG AAGGCCGAGA TGTTGATCCA 
AACAGGAGGA TGTCAGTTGC TGAGCAGGTT GACTATGTCA TTAAGGAAGC AACTAATCTA 
GATAACTTGG CTCAGCTGTA TGAAGGTTGG ACAGCCTGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg1 ( XM_008759828.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758050.1 Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X1, mRNA.

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CloneID	ORa50597
Clone ID Related Accession (Same CDS sequence)	XM_008759828.2
Accession Version	XM_008759828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 3 genomic stop codon ##RefSeq-Attributes-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATACTTTA 
AAATACTCCT CATCCAGAGA TAGGGGTGTT TCTTCCTCTT ATGGACTGCA ACCTTCAAAT 
TCAGCTGTGG TGTCTCGGCA AAGGCACGAT GATACCAGAG GCCATGCTGA CATACAGAAT 
GACGAAAAGG GTACATAGAT TTTTCTCCTG TTGGGGCATG CTTCTGTTTT AGACCTGCTC 
CTCTTTTCTA AACCCTCCTG CCAGGTAACA TCTGAGGTTC TTCCAGGCTA CTGTCTCATT 
TACTCATCTA AAATGTGCTT AAGCATCTGA GCGCTGCCTA GCAAGCGCAA GGCCCTGGAG 
CGATCGATGT CGTACTCTGA TGAGTCTCGA CTGTCGAATC TTCTTCGGAG GATCACCCGG 
GAAGATGACA GAGACCGAAG ACTGGCTACT GTAAAGCAGC TGAAGGAGTT TATTCAGCAG 
CCAGAAAATA AACTGGTACT AGTTAAACAG TTGGATAATA TCTTGGCTGC TGTGCATGAT 
GTACTAAATG AAAGTAGTAA GTTGCTTCAG GAGTTGAGGC AAGAGGGAGC TTGCTGTCTT 
GGTCTTCTCT GTGCTTCTCT GAGCTACGAG GCTGAGAAGA TCTTCAAGTG GATTTTTAGC 
AAATTTAGCT CATCTGCGAA AGATGAGGTG AAGCTCCTCT ACCTGTGTGC CACTTACAGA 
GCACTGGAGA CTGTTGGCGA GAAGAAAGCC TTTTCTTCGG TGATGCAGCT TGTGATGACC 
AGCCTACAAT CCATTCTTGA AAATGTGGAT ACACCAGAAT TACTTTGCAA ATGTGTTAAG 
TGCATTCTTT TGGTGGCTCG ATGCTACCCT CATATTTTCA GCACTAATTT TAGGGATACA 
GTTGATATAT TAGTTGGATG GCACATAGAT CATACTCAGA AACCCTCCCT TACACAGCAG 
GTGTCTGGGT GGTTGCAGAG TTTGGAGCCA TTTTGGGTAG CTGACCTTGC ATTTTCTACC 
ACTCTTCTTG GTCAATTTTT AGAAGACATG GAGGCATACG CTGAGGACCT CAGCCATGTG 
GCCTCTGGGG AGTCAGTGGA TGAAGATGTT CCTCCTCCAT CAGTGTCATT ACCAAAGCTG 
GCAGCGCTTC TCCGTGTGTT CAGTACTGTG GTGAGGAGCA TTGGGGAGCG CTTTAGCCCA 
ATTCGGGGTC CACCAATTAC CGAGGCATAT GTAACAGATG TTCTATACAG AGTAATGAGA 
TGCGTGACGG CTGCAAACCA AGTGTTTTTT TCTGAGGCTG TGTTGACAGC TGCTAATGAG 
TGTGTTGGTG TTTTGCTCGG CAGCTTGGAT CCTAGCATGA CTATACATTG TGACATGGTC 
ATTACATATG GATTAGACCA ACTGGAGAAT TGCCAGACTT GTGGTACCGA TTATATCATC 
TCAGTCTTGA ATTTGCTCAC ATTGATTGTT GAGCAGATAA ATACAAAACT CCCATCATCA 
TTTGTAGAAA AGCTTTTTAT ACCATCATCT AAACTACTAT TTCTACGATA CCACAAAGAA 
AAAGAGGTTG TTGCTGTAGC TCATGCTGTT TATCAAGCAG TGCTCAGCTT GAAGAACATC 
CCCGTTCTGG AGACTGCCTA TAAATTAATT TTGGGAGAAA TGACTTGTGC ACTAAACAAT 
TTGCTACACA GTCTCCAGCT TCCTGATGCC TGTTCTGAGA TTAAACATGA GGCTTTTCAG 
AATCATGTGT TCAACATTGA CAATGCCAAC TTTGTAGTCA TATTTGATCT CAGTGCCCTG 
ACTACAATTG GAAATGCCAA AAACTCATTA ATTGGGATGT GGGCACTGTC TCCAACTGTA 
TTTGCACTGC TGAGTAAGAA TCTGATGATA GTGCACAGTG ACCTGGCTGT TCACTTCCCT 
GCCATTCAGT ATGCGGTGCT CTACACCCTA TATTCCCATT GTACCAGACA CGATCACTTC 
ATTTCCAGTA GTCTTAGTTC TTCATCCCCT TCCTTATTTG ATGGCGCGGT GATTAGTACT 
GTAACTACAG CCACAAAGAA ACATTTCTCA ATTATATTAA ATCTTTTGGG AATATTACTA 
AAGAAAGATA ATCTTAACCA GGACACAAGG AAACTGTTAA TGACATGGGC TTTGGAAGTA 
GCTGTATTAA TGAAGAAGTC TGAAACATAT GCACCTTTAT TCTCCCTTCC ATCTTTTCAT 
AAATTCTCCA AAGGCCTTTT AGCTAACACT CTTGTTGAAG ATGTGAATAT CTGCTTGCAA 
GCATGTAGCA GTCTCCATGC TCTGTCCTCT TCCTTGCCAG ATGATCTGCT ACAGAGGTGT 
GTTGATGTCT GCCGTGTTCA GCTTGTCCAT AGTGGAACTC GTATTCGACA GGCCTTTGGA 
AAGCTGTTGA AGTCAATTCC TTTAGATGTT GTACTAAGCA ATAACAATCA CACAGAGATT 
CAGGAGATTT CTTTAGCATT GAGAAGCCAC ATGAGTAAAG CACCAAGTAA CACGTTCCAC 
CCTCAGGACT TTTCTGATGT CATCAGTTTC ATTTTGTATG GAAACTCTCA CCGAACAGGG 
AAGGACAATT GGTTAGAAAG ATTGTTCTAT AGCTGCCAGA GGCTGGATAA GCGTGACCAA 
TCCACAATTC CCCGCAATCT TCTAAAGACA GATGCTGTCC TGTGGCAGTG GGCTATTTGG 
GAAGCTGCAC AGTTCACTGT TCTTTCAAAG TTGCGAACCC CACTGGGCAG AGCTCAGGAC 
ACGTTCCAGA CAATTGAAGG TATCATTAGA AGTCTTGCAG CCCACACACT AAACCCTGAC 
CAGGATGTTA GTCAGTGGAC CACTGCAGAT AATGATGAAG GCCATGGGAG CAATCAGCTT 
AGACTTGTTC TTCTGCTTCA GTATCTGGAG AACCTGGAGA AACTAATGTA TAACGCCTAT 
GAGGGGTGTG CCAATGCATT AACATCACCT CCCAAGGTCA TTAGGACTTT TTTCTATACC 
AATCGACAGA CTTGCCAAGA CTGGCTCACT CGGATCCGTC TTTCCATCAT GAGGGTCGGA 
TTGTTGGCAG GTCAGCCTGC AGTGACAGTG AGGCACGGCT TTGACTTACT TACTGAAATG 
AAAACAAATA GTCTGTCTCA GGGGAATGAA TTGGAAGTGA CTATCATGAT GGTGGTAGAA 
GCGCTGTGTG AGCTCCACTG TCCTGAAGCT ATACAGGGGA TTGCTGTCTG GTCATCTTCA 
GCTGTTGGTA AAAACCTTCT CTGGATTAAC TCAGTGGCCC AACAGGCTGA AGGGAGGTCC 
TCTGTGGCGT ACCAGGAGCA CCTGTGTGCC ATGCCAGGTG TTGATTGCTG CATCTCCAGC 
TTTGACAAGT CTGATTCTCA CATTGGCCAA TGCTGGCGTA ACAGTGGCAG CCCGAAACAC 
TCTCTCAAAT GGTGAATCCA GAAAAATGTG CTGTCCAAAT CAATCGACTC TTCCCCTGAA 
GTTATCAACT ACCTAGGAAA TAAGGCATGT GAGTGTTACA TCTCCATTGC CGACTGGGCT 
GCTGTGCAGG AGTGGCAGAA CGCGGTCCAC GACCTGAAGA GAGAACCAAC AGCAACCTCA 
CTCAATCTGA AAGCCGATTT CACTACCATC AAGTCACTAA GCAGCTTCGA GTCTGGAGAG 
TTTGTTGAAT GCACTGAGCA GTTGGAATTA TTGCCAGGAG AAAATATTAA TCTGCTTGCT 
GGAGGGTCAA AAGAAAAAAT AGATATGAAA AAGCTGCTTC CTAACATGCT AAGCCCAGAC 
CCACGGGAGC TTCAGAAATC CATTGAAGTC CAATTGTTGA GAAGCTCCGT GTTCCTGGCA 
ACTGCTCTAA ACCATGTGGA ACAAGATCAG AAATGGCAGT CCTTAACCGA AAATGTGGTA 
AAGTACTTGA AGCAAACCTC ACGCATCGCT ATTGGACCAC TAAGATTGTC TACGCTAACG 
GTGTCACAGT CCTTGCCAGT ACTGAGTACC CTGCAGCTCT ACTGCTCCTC TGCACTGGAG 
AACACAGTTT CTAATAGACT TTCAACAGAG GACTGTCTTA TCCCACTCTT TAGTGATGCT 
CTTCGCTCAT GTAAACAGCA TGATGTGCGG CCTTGGATGC AGGCGTTGAG ATATACCATG 
TACCAGAATC AACTGTTAGA GAAAATTAAA GAACAAACAG TACCAATTAG AAGCCATCTC 
ATGGAACTAG GTCTGACAGC TGCAAAATTT GCTAGAAAAC GGGGGAATGT GTCACTGGCA 
ACAAGACTGT TGGCACAGTG CAGTGAGGTT CAGCTGGGAA AGACAACCAC TGCCCAGGAC 
TTAGTCCAGC ATTTTAAAAA ACTGTCTACT CAAGGTCAAG TGGATGAAAA ATGGGGGCCT 
GAGCTTGATA TTGAAAAAAC CAAATTGTTA TACACAGCAG GCCAGTCAAC ACACGCAATG 
GAAATGTTGA GTTCTTGTGC TATATCTTTC TGCAAATCTG CGAAAGCTGA ATATGCTGTG 
GCTAAGTCAA TTCTGACCCT GGCTAAATGG GTCCAGGCAG AATGGAAAGA GATTTCGGGA 
CAGCTGAGGC AGGTGTACCG AGCTCAGCAG CAGCAGAACC TTTCAGGCCT TTCTACTTTG 
TCTAGGAATA TACTCGCTCT TATAGAGCTA CCGTCTGCTA ATACAGTGGG AGAAGAGCAC 
CCTCGGATTG AGAGTGAGTC TACAGTGCAT ATTGGAGTTG GAGAACCTGA CTTCATTTTG 
GGACAGTTGT ATCACCTGTC TTCAGTGCAG GCACCTGAAG TAGCCAAATC TTGGGCAGCA 
TTGGCTAGTT GGGCTTATAG GTGGGGCAGA AAGGTGGTTG ACAATGCCAG TCAGGGAGAA 
GGTGTTCGTC TGTTGCCTAG AGAAAAATCT GAAGTTCAAA ATCTGCTTCC AGATACTATA 
ACTGAAGAAG AGAAAGAGAG AATATATGGT ATTCTTGGAC AAGCAGTATG TCGGCCAGCA 
GGAATTCAGG ATGAAGATAT AACACTACAG ATAACAGAGA GTGAAGATAA TGAGGATGAT 
GACATGGTCG ATGTTATCTG GCGGCAGTTA ATATCAAGCT GTCCCTGGCT TTCTGAACTG 
GATGAAAATG CAACGGAAGG TGTTATTAAA GTGTGGAGGA AAGTTGTGGA CAGAATCTTC 
AGCCTGTACA AATTGTCTTG CAGTGCATAT TTTACTTTCC TTAAGCTGAA TGCTGGTCAG 
GTTCTGTTAG ATGAGGATGA CCCCAGGCTC CATTTAAGTC ACAGAGCTGA GCAGAGCACT 
GATGATGTGA TTGTGATGGC TACCCTGCGA CTGCTCAGAT TGCTAGTGAA GCATGCTGGT 
GAGCTTCGGC AGTATCTGGA ACATGGCTTG GAGACAACCC CTACTGCTCC ATGGAGAGGA 
ATTATTCCAC AGCTTTTCTC ACGCTTAAAC CACCCTGAAG TGTATGTACG CCAGAGTATT 
TGTAACCTCC TTTGCCGTGT AGCCCAAGAT TCCCCTCACC TCATATTGTA TCCTGCAATA 
GTAGGAACCA TATCACTTAG CAGTGAATCC CAGGCTTCAG GAAATAAGTA TTCCTCTGCA 
ATCCCAACTT TACTTGGAAA TATTCAAGGA GAAGAACTGC TGGTTTCTGA ATGTGAAGGA 
GGAAGTCCTC CTGCTTCTCA GGATAGCAAC AAAGATGAAC CTAAAAGTGG TTTAAATGAA 
GATCAGGCCA TGATGCAGGA TTGCTACAGC AAAATTGTAG ATAAACTATC CTCTGCAAAT 
CCAACGATGG TTTTACAGGT CCAGATGCTT GTGGCAGAGC TACGTAGGGT CACTGTACTC 
TGGGATGAGC TCTGGCTGGG AGTTTTGTTA CAGCAGCATA TGTACGTCCT GAGGCGAATT 
CAGCAGCTGG AGGATGAGGT GAAGAGAGTC CAGAACAACA ACACCTTGCG CAAAGAAGAG 
AAAATTGCCA TCATGCGAGA GAAGCACACA GCACTGATGA AGCCCATCGT GTTTGCTTTG 
GAGCACGTGC GGAGCATCAC TGCTGCCCCT GCAGAGACCC CTCATGAAAA GTGGTTTCAG 
GATAACTATG GTGATGCCAT TGACAATGCC CTTGAAAAAC TAAAGACCCC ATCCAACCCT 
GCCAAGCCTG GAAGCAGCTG GATTCCATTT AAAGAGATAA TGCTGAGTTT GCAACAGAGA 
GCGCAGAAAC GAGCAAGCTA CATCTTGCGA CTTGATGAGA TCAGTCCTTG GTTGGCTGCC 
ATGACTAACA CTGAAATTGC ACTCCCTGGA GAAGTCTCTG CCAGAGACAC TGTGACAATT 
CACAGTGTGG GTGGAACTAT CACTATCTTA CCAACTAAAA CCAAGCCCAA GAAACTCCTC 
TTTCTTGGAT CAGATGGAAA GAGCTATCCT TACCTTTTCA AAGGTCTAGA AGATTTGCAT 
CTGGATGAGA GAATCATGCA GTTTTTATCT ATTGTGAATA CTATGTTTGC TACAATTAAT 
CGGCAGGAAA CACCCCGATT CCATGCACGG CACTATTCTG TAACACCACT GGGGACGAGA 
TCGGGGCTGA TCCAGTGGGT GGATGGAGCC ACACCATTGT TCGGGCTTTA CAAACGATGG 
CAACAGCGGG AGGCTGCCTT ACAAGCACAA AAGGCCCAAG ATTCCTACCA GACTCCTCAG 
AATCCCAGCA TTGTACCGCG TCCTAGTGAA CTTTACTATA GTAAAATTGG TCCTGCTTTG 
AAAACAGTTG GCCTTAGCCT GGATGTGTCA AGGAGGGATT GGCCCCTCCA TGTGATGAAG 
GCAGTGTTGG AGGAGTTAAT GGAGGCCACA CCCCCAAACC TTCTGGCCAA AGAACTCTGG 
TCATCTTGCA CCACTCCCGA TGAGTGGTGG AGAGTCACAC AGTCCTATGC AAGATCCACC 
GCTGTCATGT CTATGGTTGG CTACATAATT GGCCTTGGAG ACAGACATCT GGATAACGTC 
CTCATAGATA TGACAACTGG AGAAGTAGTT CACATAGATT ATAATGTTTG CTTTGAAAAA 
GGTAAAAGCC TTAGAGTTCC TGAGAAAGTG CCTTTTCGAA TGACACAAAA CATTGAAACA 
GCACTGGGTG TAACTGGAGT AGAAGGTGTT TTTAGGCTTT CCTGTGAGCA GGTTCTCCAC 
ATCATGCGAC GAGGCCGAGA GACTCTGCTG ACACTCTTGG AGGCTTTTGT GTATGACCCA 
CTGGTGGACT GGACAGCCGG CGGTGAGGCT GGGTTTGCAG GTGCTGTCTA TGGTGGAGGT 
GGCCAACAGG CCGAGAGCAA GCAGAGTAAG CGAGAGATGG AGCGTGAGAT TACCCGCAGC 
CTCTTTTCTT CCAGAGTTGC TGAGATTAAG GTGAACTGGT TTAAGAACCG GGATGAGATG 
CTAGTTGTGC TTCCCAAGCT GGACAGCAGC TTAGATGAAT ACCTAAGCCT GCAGGAGCAG 
CTGACAGATG TTGAAAAGCT GCAGGGCAAA CTGCTGGAGG AAATAGAGTT TCTAGAAGGA 
GCTGAAGGAG TGGATCATCC ATCTCGCACT CTGCAACACA GATATTCTGA GCATACTCAA 
CTACAGACCC AGCAAAGAGC TGTTCAGGAA GCAATCCAGG TGAAGCTGAA TGAGTTTGAA 
CAATGGATAA CACATTATCA GGCTGCATTC AATAACTTAG AAGCAACTCA GCTTGCAAGT 
TTGCTTCAAG AGATAAGCAC ACAAATGGAC CTTGGTCCCC CAAGCTATGT TCCAGCAACA 
GCTTTTCTAC AGAATGCTGG CCAAGCACAC CTCATCAGCC AGTGTGAGCA GCTAGAGGGG 
GAAGTTGGTA CTCTGCTGCA GCAGAGGCGC TCTGTGCTCC GTGGCTGTCT GGAACAGCTG 
CATCACTATG CAACTGTCGC TCTGCAGTAT CCGAAAGCAA TATTCCAGAA ACATCGAATT 
GAACAGTGGA AGGCCTGGAT GGAAGAGCTC ATCTGCAACA CCACAGTAGA ACGCTGCCAA 
GAACTCTATA GGAAATATGA GATGCAGTAT GCTCCTCAGC CCCCTCCAAC AGTCTGTCAG 
TTCATTACTG CCACTGAAAT GACCCTTCAG CGGTATGCAG CAGACATAAA CAGCAGACTT 
ATTAGACAAG TGGAACGCTT GAAGCAAGAA GCTGTCACTG TGCCAGTTTG TGAGGATCAG 
TTGAAAGAAA TTGAGCGTTG CATTAAAGTT TTTCTTCATG AGAATGGAGA AGAAGGATCT 
CTGAGTCTAG CAAGTGTCAT TATTTCTGCC CTTTGTACTC TTACAAGGCG CAACCTTATG 
ATGGAAGGTG CAGCTTCAAG TGCTGGAGAG CAGCTGGTTG ATCTGACATC CAGAGATGGA 
GCCTGGTTCC TAGAGGAGCT GTGTAGTATG AGTGGAAATG TCACCTGCTT GGTGCAGTTA 
CTGAAGCAGT GCCACCTGGT ACCACAGGAC TTGGATATTC CCAACCCAGT GGAAGCATCT 
GAGGCTGTTC ATTTAGCCAA TGGAGTGTAT ACATCTCTGC AGGAATTAAA TTCAAATTTC 
CGGCAAATCA TATTCCCAGA AGCTCTTAGA TGTTTAATGA AGGGGGAGTG CACTTTAGAA 
AGTATGCTAC ATGAACTAGA CAGTCTCATC GAGCAGACTA CTGATGGAGT TCCTCTGCAG 
ACTCTGGTGG AGTCGCTTCA AGCCTACTTA AGAAACACAG CCATGGGACT TGAAGAAGAA 
ACTCATGCTC ATTATATTGA TGTTGCCAGA ATGCTACATG CTCAATATGG TGAATTAATC 
CAACCAAGAA ATGGTTCAGT TGATGAAACA CCAAAAATGT CAGCTGGCCA GATGCTTTTG 
GTAGCATTTG ATGGCATGTT TGCCCAAGTT GAAACTGCTT TTGGCTTATT AGTTGAGAAG 
TTAAACAAGA TGGAGATTCC CGTTGCTTGG AGGAAGATTG ACATTATCCG GGAAGCCAGG 
AGCACCCAGG CCAATTTTTT TGATGATGAC AATCACCGGC AGGTGCTAGA AGAGATTTTC 
TTTTTAAAAA GACTGCAGAC AATTAAGGAG TTCTTCAGGC TCTGTGGTAC CTTTTCTAAA 
ACATTGTCAG GATCAAGTTC ACTTGAAGAT CAGAATACTG TAAATGGACC TGTTCAGATT 
GTCAATGTGA AAACCCTTTT TAGAAATTCT TGCTTTAGTG AAGACCAAAT GGCCAAGCCT 
ATCAAGGCCT TTACAGCTGA CTTTGTAAGA CAGCTCTTGA TAGGACTCCC AAACCAAGCC 
CTCGGACTCA CATTGTGTAG CTTTATCAGT GCCCTGGGCG TAGACATCAT TGCTCAGGTA 
GAGGCAAAGG ATTTCGGAGC TGAAAGCAAA GTCTCTGTGG ACGATCTGTG TAAGAAAGCT 
GTGGAGCACA ACATCCAGGT TGGGAGATTC TCACAGTTGG TGATGAACAG AGCTACCGTG 
TTAGCCAGCT CCTATGATAC AGCCTGGAAG AAGCATGACC TGGTGCGCAG GCTGGAAACC 
AGCATTTCTT CCTGTAAGAC AAGCTTACAG CGGGTGCAAC TGCACATTGC CATGTTCCAG 
TGGCAACATG AAGACCTCCT TATCAACAGA CCACAAGCCA TGTCAGTCAC CCCACCTCGG 
TCTGCAATCC TGACCAGCAT GAAGAAGAAA CTGCATGCTC TCAGTCAGAT TGAGACTTCA 
ATCGGGACTG TTCAGGAGAA ACTAGCAGCT CTTGAAGCAA GCATTGAGCA GCGGCTGAAG 
TGGGCAGGTG GTGCCAATCC TGCCCTGGCA CCAGTACTAC AAGATTTTGA AGCAACAATA 
GCTGAAAGAA GAAATCTTGT TCTCAAAGAG AGCCAGAGAG CAAACCAGGT CACTTTTCTC 
TGCAGCAATA TCATTCATTT TGAAAGTCTA CGAACAAGAA CTGCCGAAGC CTTAAGCCTG 
GATGCTGCCT TATTTGAACT CATCAAACGG TGTCAGCAGA TGTGTTCATT TGCATCACAG 
TTCAACAGTT CAGTTTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACGACTCTT 
GAACATCCTA TTGGCAGTTC TGAGTGGCTT TTATCAGCAC ATAAACAGTT GACCCAGGAT 
ATGTCCACCC AAAGGGCTGT TCAGACAGAG AAGGAGCAGC AGATCGAGAC TGTTTGTGAG 
ACCATCCAGA GCCTGGTTGA CAGCGTAAAG ACTGTGCTCA CGGGTCACAA CCGGCAGCTT 
GGAGATGTAA AACATCTCCT GAAAGCCATG GCTAAGGACG AAGAGGCCGC TCTAGCAGAT 
GCTGAAGATA TCCCCTATGA GAGCAGTGTC AGGCAGTTCC TGGCCGAGTA CAAATCATGG 
CAAGACAACA TTCAGACGGT TTTGTTCACA CTGGTCCAGG CCATGGGTCA GGTTCGAAGT 
CAAGAACATG TTGAAATGCT GCAGGAAATC ACTCCCACCT TGAAAGAGCT GAAGACACAA 
AGCCAGAGTA TCTATAATAA TTTAGTGAGT TTTGCATCAC CCTTAGTCAC CGACGCAGCA 
AACGAATGTT CAAGTCCAAC ATCATCTGCT ACTTACCAGC CGTCCTTTGC TGCAGCGGTT 
AGGAGTAACA CTGGCCAGAA GACTCAGCCT GATGTCATGT CTCAGAATGC TAAAAAGCTG 
ATTCAGAAAA ATCTGGCTAC ATCAGCAGAT ACTCCACCAA GCACCATCCC AGGAACTGGC 
AAGAGCATCG CCTGTAGTCC CAAAAAGGCT ATCAGAGACC CTAAAACTGG GAAAGCTGTG 
CAAGAGAGAA ACTCCTATGC AGTGAGTGTG TGGAAGAGAG TGAAAGCCAA GCTAGAAGGC 
CGAGATGTTG ATCCAAACAG GAGGATGTCA GTTGCTGAGC AGGTTGACTA TGTCATTAAG 
GAAGCAACTA ATCTAGATAA CTTGGCTCAG CTGTATGAAG GTTGGACAGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758050.1
CDS	20..10999
Translation	MSRRAPGSRLSSGGGGTKYPRSWNDWQPRTDSASADPDTLKYSSSRDRGVSSSYGLQPSNSAVVSRQRHDDTRGHADIQNDEKGTIFLLLGHASVLDLLLFSKPSCQVTSEVLPGYCLIYSSKMCLSIALPSKRKALERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYRALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFQNHVFNIDNANFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFSKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSAVGKNLLWINSVAQQAEGRSSVAYQEHLCAMPGVDCCISSFDKSDSHIGQCWRNSGSPKHSLKW*IQKNVLSKSIDSSPEVINYLGNKACECYISIADWAAVQEWQNAVHDLKREPTATSLNLKADFTTIKSLSSFESGEFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVFLATALNHVEQDQKWQSLTENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSDALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWVQAEWKEISGQLRQVYRAQQQQNLSGLSTLSRNILALIELPSANTVGEEHPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEDDDMVDVIWRQLISSCPWLSELDENATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVLLDEDDPRLHLSHRAEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKYSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIDNALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLDEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPSIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSRTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGTLLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKAWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPVEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGECTLESMLHELDSLIEQTTDGVPLQTLVESLQAYLRNTAMGLEEETHAHYIDVARMLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPVAWRKIDIIREARSTQANFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQVGRFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPRSAILTSMKKKLHALSQIETSIGTVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRANQVTFLCSNIIHFESLRTRTAEALSLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTTLEHPIGSSEWLLSAHKQLTQDMSTQRAVQTEKEQQIETVCETIQSLVDSVKTVLTGHNRQLGDVKHLLKAMAKDEEAALADAEDIPYESSVRQFLAEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDAANECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNAKKLIQKNLATSADTPPSTIPGTGKSIACSPKKAIRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_008759828.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (Smg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759828.2

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATACTTTA 
AAATACTCCT CATCCAGAGA TAGGGGTGTT TCTTCCTCTT ATGGACTGCA ACCTTCAAAT 
TCAGCTGTGG TGTCTCGGCA AAGGCACGAT GATACCAGAG GCCATGCTGA CATACAGAAT 
GACGAAAAGG GTACATAGAT TTTTCTCCTG TTGGGGCATG CTTCTGTTTT AGACCTGCTC 
CTCTTTTCTA AACCCTCCTG CCAGGTAACA TCTGAGGTTC TTCCAGGCTA CTGTCTCATT 
TACTCATCTA AAATGTGCTT AAGCATCTGA GCGCTGCCTA GCAAGCGCAA GGCCCTGGAG 
CGATCGATGT CGTACTCTGA TGAGTCTCGA CTGTCGAATC TTCTTCGGAG GATCACCCGG 
GAAGATGACA GAGACCGAAG ACTGGCTACT GTAAAGCAGC TGAAGGAGTT TATTCAGCAG 
CCAGAAAATA AACTGGTACT AGTTAAACAG TTGGATAATA TCTTGGCTGC TGTGCATGAT 
GTACTAAATG AAAGTAGTAA GTTGCTTCAG GAGTTGAGGC AAGAGGGAGC TTGCTGTCTT 
GGTCTTCTCT GTGCTTCTCT GAGCTACGAG GCTGAGAAGA TCTTCAAGTG GATTTTTAGC 
AAATTTAGCT CATCTGCGAA AGATGAGGTG AAGCTCCTCT ACCTGTGTGC CACTTACAGA 
GCACTGGAGA CTGTTGGCGA GAAGAAAGCC TTTTCTTCGG TGATGCAGCT TGTGATGACC 
AGCCTACAAT CCATTCTTGA AAATGTGGAT ACACCAGAAT TACTTTGCAA ATGTGTTAAG 
TGCATTCTTT TGGTGGCTCG ATGCTACCCT CATATTTTCA GCACTAATTT TAGGGATACA 
GTTGATATAT TAGTTGGATG GCACATAGAT CATACTCAGA AACCCTCCCT TACACAGCAG 
GTGTCTGGGT GGTTGCAGAG TTTGGAGCCA TTTTGGGTAG CTGACCTTGC ATTTTCTACC 
ACTCTTCTTG GTCAATTTTT AGAAGACATG GAGGCATACG CTGAGGACCT CAGCCATGTG 
GCCTCTGGGG AGTCAGTGGA TGAAGATGTT CCTCCTCCAT CAGTGTCATT ACCAAAGCTG 
GCAGCGCTTC TCCGTGTGTT CAGTACTGTG GTGAGGAGCA TTGGGGAGCG CTTTAGCCCA 
ATTCGGGGTC CACCAATTAC CGAGGCATAT GTAACAGATG TTCTATACAG AGTAATGAGA 
TGCGTGACGG CTGCAAACCA AGTGTTTTTT TCTGAGGCTG TGTTGACAGC TGCTAATGAG 
TGTGTTGGTG TTTTGCTCGG CAGCTTGGAT CCTAGCATGA CTATACATTG TGACATGGTC 
ATTACATATG GATTAGACCA ACTGGAGAAT TGCCAGACTT GTGGTACCGA TTATATCATC 
TCAGTCTTGA ATTTGCTCAC ATTGATTGTT GAGCAGATAA ATACAAAACT CCCATCATCA 
TTTGTAGAAA AGCTTTTTAT ACCATCATCT AAACTACTAT TTCTACGATA CCACAAAGAA 
AAAGAGGTTG TTGCTGTAGC TCATGCTGTT TATCAAGCAG TGCTCAGCTT GAAGAACATC 
CCCGTTCTGG AGACTGCCTA TAAATTAATT TTGGGAGAAA TGACTTGTGC ACTAAACAAT 
TTGCTACACA GTCTCCAGCT TCCTGATGCC TGTTCTGAGA TTAAACATGA GGCTTTTCAG 
AATCATGTGT TCAACATTGA CAATGCCAAC TTTGTAGTCA TATTTGATCT CAGTGCCCTG 
ACTACAATTG GAAATGCCAA AAACTCATTA ATTGGGATGT GGGCACTGTC TCCAACTGTA 
TTTGCACTGC TGAGTAAGAA TCTGATGATA GTGCACAGTG ACCTGGCTGT TCACTTCCCT 
GCCATTCAGT ATGCGGTGCT CTACACCCTA TATTCCCATT GTACCAGACA CGATCACTTC 
ATTTCCAGTA GTCTTAGTTC TTCATCCCCT TCCTTATTTG ATGGCGCGGT GATTAGTACT 
GTAACTACAG CCACAAAGAA ACATTTCTCA ATTATATTAA ATCTTTTGGG AATATTACTA 
AAGAAAGATA ATCTTAACCA GGACACAAGG AAACTGTTAA TGACATGGGC TTTGGAAGTA 
GCTGTATTAA TGAAGAAGTC TGAAACATAT GCACCTTTAT TCTCCCTTCC ATCTTTTCAT 
AAATTCTCCA AAGGCCTTTT AGCTAACACT CTTGTTGAAG ATGTGAATAT CTGCTTGCAA 
GCATGTAGCA GTCTCCATGC TCTGTCCTCT TCCTTGCCAG ATGATCTGCT ACAGAGGTGT 
GTTGATGTCT GCCGTGTTCA GCTTGTCCAT AGTGGAACTC GTATTCGACA GGCCTTTGGA 
AAGCTGTTGA AGTCAATTCC TTTAGATGTT GTACTAAGCA ATAACAATCA CACAGAGATT 
CAGGAGATTT CTTTAGCATT GAGAAGCCAC ATGAGTAAAG CACCAAGTAA CACGTTCCAC 
CCTCAGGACT TTTCTGATGT CATCAGTTTC ATTTTGTATG GAAACTCTCA CCGAACAGGG 
AAGGACAATT GGTTAGAAAG ATTGTTCTAT AGCTGCCAGA GGCTGGATAA GCGTGACCAA 
TCCACAATTC CCCGCAATCT TCTAAAGACA GATGCTGTCC TGTGGCAGTG GGCTATTTGG 
GAAGCTGCAC AGTTCACTGT TCTTTCAAAG TTGCGAACCC CACTGGGCAG AGCTCAGGAC 
ACGTTCCAGA CAATTGAAGG TATCATTAGA AGTCTTGCAG CCCACACACT AAACCCTGAC 
CAGGATGTTA GTCAGTGGAC CACTGCAGAT AATGATGAAG GCCATGGGAG CAATCAGCTT 
AGACTTGTTC TTCTGCTTCA GTATCTGGAG AACCTGGAGA AACTAATGTA TAACGCCTAT 
GAGGGGTGTG CCAATGCATT AACATCACCT CCCAAGGTCA TTAGGACTTT TTTCTATACC 
AATCGACAGA CTTGCCAAGA CTGGCTCACT CGGATCCGTC TTTCCATCAT GAGGGTCGGA 
TTGTTGGCAG GTCAGCCTGC AGTGACAGTG AGGCACGGCT TTGACTTACT TACTGAAATG 
AAAACAAATA GTCTGTCTCA GGGGAATGAA TTGGAAGTGA CTATCATGAT GGTGGTAGAA 
GCGCTGTGTG AGCTCCACTG TCCTGAAGCT ATACAGGGGA TTGCTGTCTG GTCATCTTCA 
GCTGTTGGTA AAAACCTTCT CTGGATTAAC TCAGTGGCCC AACAGGCTGA AGGGAGGTCC 
TCTGTGGCGT ACCAGGAGCA CCTGTGTGCC ATGCCAGGTG TTGATTGCTG CATCTCCAGC 
TTTGACAAGT CTGATTCTCA CATTGGCCAA TGCTGGCGTA ACAGTGGCAG CCCGAAACAC 
TCTCTCAAAT GGTGAATCCA GAAAAATGTG CTGTCCAAAT CAATCGACTC TTCCCCTGAA 
GTTATCAACT ACCTAGGAAA TAAGGCATGT GAGTGTTACA TCTCCATTGC CGACTGGGCT 
GCTGTGCAGG AGTGGCAGAA CGCGGTCCAC GACCTGAAGA GAGAACCAAC AGCAACCTCA 
CTCAATCTGA AAGCCGATTT CACTACCATC AAGTCACTAA GCAGCTTCGA GTCTGGAGAG 
TTTGTTGAAT GCACTGAGCA GTTGGAATTA TTGCCAGGAG AAAATATTAA TCTGCTTGCT 
GGAGGGTCAA AAGAAAAAAT AGATATGAAA AAGCTGCTTC CTAACATGCT AAGCCCAGAC 
CCACGGGAGC TTCAGAAATC CATTGAAGTC CAATTGTTGA GAAGCTCCGT GTTCCTGGCA 
ACTGCTCTAA ACCATGTGGA ACAAGATCAG AAATGGCAGT CCTTAACCGA AAATGTGGTA 
AAGTACTTGA AGCAAACCTC ACGCATCGCT ATTGGACCAC TAAGATTGTC TACGCTAACG 
GTGTCACAGT CCTTGCCAGT ACTGAGTACC CTGCAGCTCT ACTGCTCCTC TGCACTGGAG 
AACACAGTTT CTAATAGACT TTCAACAGAG GACTGTCTTA TCCCACTCTT TAGTGATGCT 
CTTCGCTCAT GTAAACAGCA TGATGTGCGG CCTTGGATGC AGGCGTTGAG ATATACCATG 
TACCAGAATC AACTGTTAGA GAAAATTAAA GAACAAACAG TACCAATTAG AAGCCATCTC 
ATGGAACTAG GTCTGACAGC TGCAAAATTT GCTAGAAAAC GGGGGAATGT GTCACTGGCA 
ACAAGACTGT TGGCACAGTG CAGTGAGGTT CAGCTGGGAA AGACAACCAC TGCCCAGGAC 
TTAGTCCAGC ATTTTAAAAA ACTGTCTACT CAAGGTCAAG TGGATGAAAA ATGGGGGCCT 
GAGCTTGATA TTGAAAAAAC CAAATTGTTA TACACAGCAG GCCAGTCAAC ACACGCAATG 
GAAATGTTGA GTTCTTGTGC TATATCTTTC TGCAAATCTG CGAAAGCTGA ATATGCTGTG 
GCTAAGTCAA TTCTGACCCT GGCTAAATGG GTCCAGGCAG AATGGAAAGA GATTTCGGGA 
CAGCTGAGGC AGGTGTACCG AGCTCAGCAG CAGCAGAACC TTTCAGGCCT TTCTACTTTG 
TCTAGGAATA TACTCGCTCT TATAGAGCTA CCGTCTGCTA ATACAGTGGG AGAAGAGCAC 
CCTCGGATTG AGAGTGAGTC TACAGTGCAT ATTGGAGTTG GAGAACCTGA CTTCATTTTG 
GGACAGTTGT ATCACCTGTC TTCAGTGCAG GCACCTGAAG TAGCCAAATC TTGGGCAGCA 
TTGGCTAGTT GGGCTTATAG GTGGGGCAGA AAGGTGGTTG ACAATGCCAG TCAGGGAGAA 
GGTGTTCGTC TGTTGCCTAG AGAAAAATCT GAAGTTCAAA ATCTGCTTCC AGATACTATA 
ACTGAAGAAG AGAAAGAGAG AATATATGGT ATTCTTGGAC AAGCAGTATG TCGGCCAGCA 
GGAATTCAGG ATGAAGATAT AACACTACAG ATAACAGAGA GTGAAGATAA TGAGGATGAT 
GACATGGTCG ATGTTATCTG GCGGCAGTTA ATATCAAGCT GTCCCTGGCT TTCTGAACTG 
GATGAAAATG CAACGGAAGG TGTTATTAAA GTGTGGAGGA AAGTTGTGGA CAGAATCTTC 
AGCCTGTACA AATTGTCTTG CAGTGCATAT TTTACTTTCC TTAAGCTGAA TGCTGGTCAG 
GTTCTGTTAG ATGAGGATGA CCCCAGGCTC CATTTAAGTC ACAGAGCTGA GCAGAGCACT 
GATGATGTGA TTGTGATGGC TACCCTGCGA CTGCTCAGAT TGCTAGTGAA GCATGCTGGT 
GAGCTTCGGC AGTATCTGGA ACATGGCTTG GAGACAACCC CTACTGCTCC ATGGAGAGGA 
ATTATTCCAC AGCTTTTCTC ACGCTTAAAC CACCCTGAAG TGTATGTACG CCAGAGTATT 
TGTAACCTCC TTTGCCGTGT AGCCCAAGAT TCCCCTCACC TCATATTGTA TCCTGCAATA 
GTAGGAACCA TATCACTTAG CAGTGAATCC CAGGCTTCAG GAAATAAGTA TTCCTCTGCA 
ATCCCAACTT TACTTGGAAA TATTCAAGGA GAAGAACTGC TGGTTTCTGA ATGTGAAGGA 
GGAAGTCCTC CTGCTTCTCA GGATAGCAAC AAAGATGAAC CTAAAAGTGG TTTAAATGAA 
GATCAGGCCA TGATGCAGGA TTGCTACAGC AAAATTGTAG ATAAACTATC CTCTGCAAAT 
CCAACGATGG TTTTACAGGT CCAGATGCTT GTGGCAGAGC TACGTAGGGT CACTGTACTC 
TGGGATGAGC TCTGGCTGGG AGTTTTGTTA CAGCAGCATA TGTACGTCCT GAGGCGAATT 
CAGCAGCTGG AGGATGAGGT GAAGAGAGTC CAGAACAACA ACACCTTGCG CAAAGAAGAG 
AAAATTGCCA TCATGCGAGA GAAGCACACA GCACTGATGA AGCCCATCGT GTTTGCTTTG 
GAGCACGTGC GGAGCATCAC TGCTGCCCCT GCAGAGACCC CTCATGAAAA GTGGTTTCAG 
GATAACTATG GTGATGCCAT TGACAATGCC CTTGAAAAAC TAAAGACCCC ATCCAACCCT 
GCCAAGCCTG GAAGCAGCTG GATTCCATTT AAAGAGATAA TGCTGAGTTT GCAACAGAGA 
GCGCAGAAAC GAGCAAGCTA CATCTTGCGA CTTGATGAGA TCAGTCCTTG GTTGGCTGCC 
ATGACTAACA CTGAAATTGC ACTCCCTGGA GAAGTCTCTG CCAGAGACAC TGTGACAATT 
CACAGTGTGG GTGGAACTAT CACTATCTTA CCAACTAAAA CCAAGCCCAA GAAACTCCTC 
TTTCTTGGAT CAGATGGAAA GAGCTATCCT TACCTTTTCA AAGGTCTAGA AGATTTGCAT 
CTGGATGAGA GAATCATGCA GTTTTTATCT ATTGTGAATA CTATGTTTGC TACAATTAAT 
CGGCAGGAAA CACCCCGATT CCATGCACGG CACTATTCTG TAACACCACT GGGGACGAGA 
TCGGGGCTGA TCCAGTGGGT GGATGGAGCC ACACCATTGT TCGGGCTTTA CAAACGATGG 
CAACAGCGGG AGGCTGCCTT ACAAGCACAA AAGGCCCAAG ATTCCTACCA GACTCCTCAG 
AATCCCAGCA TTGTACCGCG TCCTAGTGAA CTTTACTATA GTAAAATTGG TCCTGCTTTG 
AAAACAGTTG GCCTTAGCCT GGATGTGTCA AGGAGGGATT GGCCCCTCCA TGTGATGAAG 
GCAGTGTTGG AGGAGTTAAT GGAGGCCACA CCCCCAAACC TTCTGGCCAA AGAACTCTGG 
TCATCTTGCA CCACTCCCGA TGAGTGGTGG AGAGTCACAC AGTCCTATGC AAGATCCACC 
GCTGTCATGT CTATGGTTGG CTACATAATT GGCCTTGGAG ACAGACATCT GGATAACGTC 
CTCATAGATA TGACAACTGG AGAAGTAGTT CACATAGATT ATAATGTTTG CTTTGAAAAA 
GGTAAAAGCC TTAGAGTTCC TGAGAAAGTG CCTTTTCGAA TGACACAAAA CATTGAAACA 
GCACTGGGTG TAACTGGAGT AGAAGGTGTT TTTAGGCTTT CCTGTGAGCA GGTTCTCCAC 
ATCATGCGAC GAGGCCGAGA GACTCTGCTG ACACTCTTGG AGGCTTTTGT GTATGACCCA 
CTGGTGGACT GGACAGCCGG CGGTGAGGCT GGGTTTGCAG GTGCTGTCTA TGGTGGAGGT 
GGCCAACAGG CCGAGAGCAA GCAGAGTAAG CGAGAGATGG AGCGTGAGAT TACCCGCAGC 
CTCTTTTCTT CCAGAGTTGC TGAGATTAAG GTGAACTGGT TTAAGAACCG GGATGAGATG 
CTAGTTGTGC TTCCCAAGCT GGACAGCAGC TTAGATGAAT ACCTAAGCCT GCAGGAGCAG 
CTGACAGATG TTGAAAAGCT GCAGGGCAAA CTGCTGGAGG AAATAGAGTT TCTAGAAGGA 
GCTGAAGGAG TGGATCATCC ATCTCGCACT CTGCAACACA GATATTCTGA GCATACTCAA 
CTACAGACCC AGCAAAGAGC TGTTCAGGAA GCAATCCAGG TGAAGCTGAA TGAGTTTGAA 
CAATGGATAA CACATTATCA GGCTGCATTC AATAACTTAG AAGCAACTCA GCTTGCAAGT 
TTGCTTCAAG AGATAAGCAC ACAAATGGAC CTTGGTCCCC CAAGCTATGT TCCAGCAACA 
GCTTTTCTAC AGAATGCTGG CCAAGCACAC CTCATCAGCC AGTGTGAGCA GCTAGAGGGG 
GAAGTTGGTA CTCTGCTGCA GCAGAGGCGC TCTGTGCTCC GTGGCTGTCT GGAACAGCTG 
CATCACTATG CAACTGTCGC TCTGCAGTAT CCGAAAGCAA TATTCCAGAA ACATCGAATT 
GAACAGTGGA AGGCCTGGAT GGAAGAGCTC ATCTGCAACA CCACAGTAGA ACGCTGCCAA 
GAACTCTATA GGAAATATGA GATGCAGTAT GCTCCTCAGC CCCCTCCAAC AGTCTGTCAG 
TTCATTACTG CCACTGAAAT GACCCTTCAG CGGTATGCAG CAGACATAAA CAGCAGACTT 
ATTAGACAAG TGGAACGCTT GAAGCAAGAA GCTGTCACTG TGCCAGTTTG TGAGGATCAG 
TTGAAAGAAA TTGAGCGTTG CATTAAAGTT TTTCTTCATG AGAATGGAGA AGAAGGATCT 
CTGAGTCTAG CAAGTGTCAT TATTTCTGCC CTTTGTACTC TTACAAGGCG CAACCTTATG 
ATGGAAGGTG CAGCTTCAAG TGCTGGAGAG CAGCTGGTTG ATCTGACATC CAGAGATGGA 
GCCTGGTTCC TAGAGGAGCT GTGTAGTATG AGTGGAAATG TCACCTGCTT GGTGCAGTTA 
CTGAAGCAGT GCCACCTGGT ACCACAGGAC TTGGATATTC CCAACCCAGT GGAAGCATCT 
GAGGCTGTTC ATTTAGCCAA TGGAGTGTAT ACATCTCTGC AGGAATTAAA TTCAAATTTC 
CGGCAAATCA TATTCCCAGA AGCTCTTAGA TGTTTAATGA AGGGGGAGTG CACTTTAGAA 
AGTATGCTAC ATGAACTAGA CAGTCTCATC GAGCAGACTA CTGATGGAGT TCCTCTGCAG 
ACTCTGGTGG AGTCGCTTCA AGCCTACTTA AGAAACACAG CCATGGGACT TGAAGAAGAA 
ACTCATGCTC ATTATATTGA TGTTGCCAGA ATGCTACATG CTCAATATGG TGAATTAATC 
CAACCAAGAA ATGGTTCAGT TGATGAAACA CCAAAAATGT CAGCTGGCCA GATGCTTTTG 
GTAGCATTTG ATGGCATGTT TGCCCAAGTT GAAACTGCTT TTGGCTTATT AGTTGAGAAG 
TTAAACAAGA TGGAGATTCC CGTTGCTTGG AGGAAGATTG ACATTATCCG GGAAGCCAGG 
AGCACCCAGG CCAATTTTTT TGATGATGAC AATCACCGGC AGGTGCTAGA AGAGATTTTC 
TTTTTAAAAA GACTGCAGAC AATTAAGGAG TTCTTCAGGC TCTGTGGTAC CTTTTCTAAA 
ACATTGTCAG GATCAAGTTC ACTTGAAGAT CAGAATACTG TAAATGGACC TGTTCAGATT 
GTCAATGTGA AAACCCTTTT TAGAAATTCT TGCTTTAGTG AAGACCAAAT GGCCAAGCCT 
ATCAAGGCCT TTACAGCTGA CTTTGTAAGA CAGCTCTTGA TAGGACTCCC AAACCAAGCC 
CTCGGACTCA CATTGTGTAG CTTTATCAGT GCCCTGGGCG TAGACATCAT TGCTCAGGTA 
GAGGCAAAGG ATTTCGGAGC TGAAAGCAAA GTCTCTGTGG ACGATCTGTG TAAGAAAGCT 
GTGGAGCACA ACATCCAGGT TGGGAGATTC TCACAGTTGG TGATGAACAG AGCTACCGTG 
TTAGCCAGCT CCTATGATAC AGCCTGGAAG AAGCATGACC TGGTGCGCAG GCTGGAAACC 
AGCATTTCTT CCTGTAAGAC AAGCTTACAG CGGGTGCAAC TGCACATTGC CATGTTCCAG 
TGGCAACATG AAGACCTCCT TATCAACAGA CCACAAGCCA TGTCAGTCAC CCCACCTCGG 
TCTGCAATCC TGACCAGCAT GAAGAAGAAA CTGCATGCTC TCAGTCAGAT TGAGACTTCA 
ATCGGGACTG TTCAGGAGAA ACTAGCAGCT CTTGAAGCAA GCATTGAGCA GCGGCTGAAG 
TGGGCAGGTG GTGCCAATCC TGCCCTGGCA CCAGTACTAC AAGATTTTGA AGCAACAATA 
GCTGAAAGAA GAAATCTTGT TCTCAAAGAG AGCCAGAGAG CAAACCAGGT CACTTTTCTC 
TGCAGCAATA TCATTCATTT TGAAAGTCTA CGAACAAGAA CTGCCGAAGC CTTAAGCCTG 
GATGCTGCCT TATTTGAACT CATCAAACGG TGTCAGCAGA TGTGTTCATT TGCATCACAG 
TTCAACAGTT CAGTTTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACGACTCTT 
GAACATCCTA TTGGCAGTTC TGAGTGGCTT TTATCAGCAC ATAAACAGTT GACCCAGGAT 
ATGTCCACCC AAAGGGCTGT TCAGACAGAG AAGGAGCAGC AGATCGAGAC TGTTTGTGAG 
ACCATCCAGA GCCTGGTTGA CAGCGTAAAG ACTGTGCTCA CGGGTCACAA CCGGCAGCTT 
GGAGATGTAA AACATCTCCT GAAAGCCATG GCTAAGGACG AAGAGGCCGC TCTAGCAGAT 
GCTGAAGATA TCCCCTATGA GAGCAGTGTC AGGCAGTTCC TGGCCGAGTA CAAATCATGG 
CAAGACAACA TTCAGACGGT TTTGTTCACA CTGGTCCAGG CCATGGGTCA GGTTCGAAGT 
CAAGAACATG TTGAAATGCT GCAGGAAATC ACTCCCACCT TGAAAGAGCT GAAGACACAA 
AGCCAGAGTA TCTATAATAA TTTAGTGAGT TTTGCATCAC CCTTAGTCAC CGACGCAGCA 
AACGAATGTT CAAGTCCAAC ATCATCTGCT ACTTACCAGC CGTCCTTTGC TGCAGCGGTT 
AGGAGTAACA CTGGCCAGAA GACTCAGCCT GATGTCATGT CTCAGAATGC TAAAAAGCTG 
ATTCAGAAAA ATCTGGCTAC ATCAGCAGAT ACTCCACCAA GCACCATCCC AGGAACTGGC 
AAGAGCATCG CCTGTAGTCC CAAAAAGGCT ATCAGAGACC CTAAAACTGG GAAAGCTGTG 
CAAGAGAGAA ACTCCTATGC AGTGAGTGTG TGGAAGAGAG TGAAAGCCAA GCTAGAAGGC 
CGAGATGTTG ATCCAAACAG GAGGATGTCA GTTGCTGAGC AGGTTGACTA TGTCATTAAG 
GAAGCAACTA ATCTAGATAA CTTGGCTCAG CTGTATGAAG GTTGGACAGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Smg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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