RP11-345J4.5 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	RP11-345J4.5
Entrez Gene ID	416605
Full Name	SMG1, nonsense mediated mRNA decay associated PI3K related kinase
General protein information	Preferred Names SMG1, nonsense mediated mRNA decay associated PI3K related kinase Names serine/threonine-protein kinase SMG1 SMG1 homolog, phosphatidylinositol 3-kinase-related kinase SMG1 phosphatidylinositol 3-kinase-related kinase smg-1 homolog, phosphatidylinositol 3-kinase-related kinase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015294535.1	XP_015150021.1	serine/threonine-protein kinase SMG1 isoform X2
XM_414907.6	XP_414907.4	serine/threonine-protein kinase SMG1 isoform X1
XM_015294535.2	XP_015150021.1	serine/threonine-protein kinase SMG1 isoform X2
XM_414907.5	XP_414907.4	serine/threonine-protein kinase SMG1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMG1	XP_851552.2
Bos taurus (cattle)	SMG1	NP_001192844.1
Pan troglodytes (chimpanzee)	SMG1	XP_001152224.2
Homo sapiens (human)	SMG1	NP_055907.3
Rattus norvegicus (Norway rat)	Smg1	XP_002728837.2
Gallus gallus (chicken)	SMG1	XP_414907.3
Mus musculus (house mouse)	Smg1	NP_001026984.1
Macaca mulatta (Rhesus monkey)	LOC693542	XP_002808335.1
Danio rerio (zebrafish)	smg1	NP_001073513.2
Xenopus tropicalis (tropical clawed frog)	smg1	XP_002932267.2

Gene Ontology

The following RP11-345J4.5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RP11-345J4.5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa144591	XM_414907.6 Latest version!	Gallus gallus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa144592	XM_015294535.2 Latest version!	Gallus gallus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa42447	XM_414907.5 Latest version!	Gallus gallus Uncharacterized protein (RP11-345J4.5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa42448	XM_015294535.1 Latest version!	Gallus gallus Uncharacterized protein (RP11-345J4.5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RP11-345J4.5 ( XM_414907.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_414907.4 Gallus gallus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

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CloneID	OGa144591
Clone ID Related Accession (Same CDS sequence)	XM_414907.6
Accession Version	XM_414907.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_414907.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCGGCACCG GCAGCAGGCA GCACTACCAC 
CCGCGGGGCT GGAATGACTG GCAACCCAGA ACTGACAGTG CATCAGCTGA CCAGGACAAT 
TTAAAATACT CTTCATCCAG AGATCGGGGC AATTCTGCCT CCTATGGATT ACAACCCTCA 
AATTCAGCTG TGGTGTCTCG ACAAAGACAC GATGATATCA GAGTCCACAC TGACATACAG 
AATGAAGACA AAGGTGGCTA CAGTGTCAAT GGAGGATCTG GGGAAAATAC TTATGGTCGG 
AAGTCGTTGG GGCAAGAGCT GAGAGTTAAC AATGTGACCA ATCCTGATTT TACCAGTGTT 
CCGCATGGGA ATCGTGCTTT AGCCACAAAA GACATGAGGA AATCACAGGA GCGATCGTTG 
TCTTATTCTG ACGAGTCTCG ACTGTCAAAT CTTCTTCGGA GGATTACTCG GGAAGACGAC 
AGAGACCGAA GACTGGCTAC TGTAAAGCAG CTGAAAGAAT TCATTCAGCA ACCAGAAAAC 
AAACTGGTAC TAGTTAAACA ACTGGATAAT ATCCTGACTG CCATTCATGA TGTACTCAAT 
GAAAGTAGCA AATTGCTTCA AGAACTGAGA CAGGAAGGAG CTTGCTGTCT TGGCCTTCTT 
TGTGCTTCTC TAAGCTATGA GGCTGAGAAG ATATTTAAAT GGATTTTTAG CAAATTCAGC 
TTATCCACAA AAGATGAAGT TAAACTTCTT TATTTGTGTG CAACCTACAA AGCACTGGAG 
ACTGTAGGAG AAAAGAAAGC CTTTTCATCT TTAATGCAGC TTGTAATGAC CAGCCTACAG 
TCCATTCTAG AAAATGTGGA TACACCAGAG TTACTGTGCA AATGTGTCAA GTGCATTCTT 
CTGTTGTCCC GATGTTATCC GCACATTTTC AGCACCAATT TTCGGGACAC AGTGGACATA 
CTGGTTGGGT GGCATATAGA TCACACTCAG AAGCCTTCAT TGACTCAGCA GGTGTCCGGG 
TGGCTGCAGA GCTTGGAGCC CTTTTGGGTG GCTGATCTCA CTTTTTCTAC CACACTCTTG 
GGTCAATTTT TAGAAGATAT GGAAGCTTAT GCTGAGGACC TCAGCCATGT GGCTTCTGGG 
GAGTCCCTAG ATGAAGATAT TCCTCCTCCC TCTGTATCAC TACCTAAACT GGCTGCGCTT 
CTTCGAGTTT TCAGTACTGT GGTGAGAAGT ATTGGGGAGC GCTTCAGCCC AATTAGAGGA 
CCACCGATTA CAGAAGCATA TGTAACTGAT GTTCTGTACA GAGTAATGAG ATGTGTGACT 
GCAGCAAACC AAGTATTCTT TTCAGAAGCA GTACTCACAG CTGCCAATGA GTGTGTTGGT 
GTTTTACTTG GCAGTCTGGA TCCAAGCATG ACCATTCACT GTGACATGGT TATCACCTAC 
GGATTGGATC AAATGGAAAA TTGTCAGACT TGTGGCACTG ATTATATCAT TTCAGTCCTG 
AATCTGTTGA CGCTGATTGT TGAACAAATA AATACAAAAC TACCATCGTC ATTTGTAGAG 
AAATTATTTA TTCCAGAGTC TAAACTGCTT GTACTTCGTT ATCATAAGGA AAAAGAGGTT 
GTTGCAGCAG CCCACGCTGT GTATCAGGCA GTACTGAGCC TGAAGAACAT TCCTGTTTTG 
GAGACTGCTT ACAGGTTGAT TCTAGGAGAA ATGACCTGTG CTCTAAACAG TCTTCTTTAT 
AGTTTACATC TTCCCGAGGC CTGTTCTGAA ATCCAGCATG ACTCTTTCAA GAAGCATGTA 
TTCACTGTGG AAAATGCAAA GTTTGTTGTT ATATTTGACC TCAGTGCCCT AAGTACTATT 
GGAAATGCTA AGAACTCATT AATTGGGATG TGGGCCCTTT CGCCAACTGT GTTTGCACTA 
CTGAGTAAAA ATCTGATGAT TGTTCATGGT GACATAGCAG TTCATTTTCC TGCTGTCCAG 
TATGCAGTAC TCTATACGTT GTATTCGCAT TGTTCCAGAC ATGACCATTT TATATCCAGT 
AGCCTCAGTT CTTCCTCTCC TTCCCTGTTC GATGGTGCAG TTATTAGTAC TGTAACCACA 
GCAACAAAAA AACATTTCTC AATCATACTG AACCTTTTGG GGCTACTGTT GAAGAAGGAT 
AACCTTACTC AAGATACCAG GAAACTGCTT ATGACGTGGG CTTTGGAAGT GGCTGTTCTG 
ATGAAGAAAT CAGAGACATA TGCACCCTTA TTTTCTCTCC CATCTTTCCA TAAGTTTTGC 
AAAGGACTTC TAGCAAACAC CCTCCTGGAA GATGTGAATA TTTGTCTTCA AGCGTGTAGC 
AGCCTCCATG CCCTGTCCTC CTCCCTACCA GATGACCTTT TACAGAGATG TGTTGATGTG 
TGTCGTGTTC AACTAGTTCA TAGCGGTGCT CGTGTAAGGC AGACTTTCGG AAAACTTCTG 
AAGTCAATTC CCTTAGATGT GGTGTTGAGC AACCGTAACC ACACAGAAAT ACAAGAAATT 
TCTTTGGCTA TAAGAAGTCA TATGAGCAAA GCTCCAAGTA ATACATTCCA CCCACAGGAT 
TTCTCTGATG TCATTAGTTT TATTTTGTAT GGAAGCACTC ATCGAACTGG GAAAGATAAT 
TGGTTGGAAA GGTTATTCTA TAGCTGTCAA AGGCTGGATA AACGAGATCA ACCAACCATC 
CCTCGTAATC TGTTGAAGAC TGATGCTGTT CTCTGGCAAT GGGCTGTTTG GGAAGCAGCT 
CAGTTTACTG TTCTTTCTAA GCTGAGAACT CCTCTGGGTA GAGCCCAAGA TACATTTCAA 
ACAATTGAAG GTATTATTAG GAGTCTTGCA GCGCATACGC TAAACCCTGA TCAAGATGTT 
AGCCAGTGGA CTACTGCAGA CAATGATGAG GGACACAGCA GCAATCAACT TAGGCTTGTT 
CTCCTTCTAC AGTATCTGGA GAACTTGGAA AAACTGATGT ACAATGCTTA TGAAGGATGT 
GCCAATGCCC TTACCTCTCC ACCCAAGGTT ATCAGGACAT TCTTTTATAC TAATCGCCAG 
ACGTGCCAAG ATTGGCTGAC GCGGATTAGA CTTTCCATCA TGAGAGTTGG ACTGCTGGCC 
GGCCAGCCTG CTGTGACAGT CAGACATGGT TTTGACTTAC TCACAGAAAT GAAAACTAAT 
AACAGTTTAT CTCAGGGAAA TGAATTGGAA GTGACAATTA TGATGCTGGT GGAAGCATTA 
TGTGAACTTC ACTGCCCTGA AGCTATTCAG GGTATTGCTG TCTGGTCTTC TGCTGTAGTC 
GGGAAGAGTC TTACCTGGAT CAACTCTGTA GCTCAACAGG CAGAAGGGCG GTTTGAAAAA 
GCAACTGCGG AGTACCAGGA GCACCTGTGT GCCATGACAG GAGTAGATTG CTGTATATCG 
GGATTTGACA AAACTGTTCT GAAGTTGGCC AATTCCAACA GTGTGAATAA CGCCAGCCCA 
AAGCACTCCT TGAACGGAGA AACTAGAAAA ACTGTTCTGA CTAAGCCAAG TGACTGTTCA 
CCTGAAGTTA TAAATTACTT GGGTAACAAG GCATGTGAAT GTTACATTTC AATTGCTGAT 
TGGTCTGCTG TTCAGGAATG GCAAAATATG GTCCATGACT TGAAGAAAAG CACGAGTAAT 
ACCTCAATCA ATCTGAAAGC AGATTTTAAC TACATCAAAT CCCTGAGCAG CTTTGAGTCT 
GGTGAACTTA CTGAATGCAC TGAACAACTG GAATTATTAC CAGGAGAAAA TATCAACCTA 
CTTGCAGGGG GATCCAAAGA AAAAATAGAC ATGAAGAAGC TCCTTCCTAA TATGCTAAGT 
CCTGACCCGA GAGAACTTCA AAAAGCAGTT GAAGTACAAC TTCTGAGAAG TTCGGTGTGT 
CTGGCAACAG CTGTAAACCA CATGGAGCAG GAGCAAAAGT GGCAGTCAAT ATCTGAAAAT 
GTTATAAAAT ACTTAAAGCA AACATCACGA ATAGCTATTG GGCCTTTAAG ACTTTCCACG 
CTCACCGTGT CTCAGTCTTT ACCAGTGTTG AGCACTCTTC AGTTGTATTG TTCTTCTGCT 
TTAGAAAACA CCGTATCCAA CAGGCTGACA TCAGAGGACT GTCTTATACC TCTCTTCAAT 
GATGCTCTGA GGTCATGTAA ACAGCATGAT GTGAGGCCGT GGATGCATGC ACTGAGATAC 
ACGGTGTATC AGAATCAACT GATGGGAAAG CTTAAAGAAC CAACAGTCCC AATTAAGAGT 
CATCTAATGG AGCTGGGCTT AACAGCAGCA AAATTTGCAC GGAAGCGAGG AAATATTGCC 
CTGGCTACAC GGTTGCTTGC CCAGTGCAGT GAAGTTCAGC TAGGAAAAAC CACCACTGCA 
CAGGACTTAG TCCAGCACTT TAAGAAACTG TCTGCACCAG GTCAGATTGA TGAAAAGTGG 
GGTCCTGAAC TTGATATTGA GAAAACAAAG CTGTTATACA CAGCAGGTCA GTCAACACAT 
GCCATGGAAA TGCTGAGTTC TTGTGCTATA TCATTTTGCA AATCTGCCAA GGCTGAATAT 
GCAGTTGCTA AATCTATTCT TACGCTGGCT AAATGGATTC AAGCAGAATG GAAAGAGATT 
TCAGGACAGT TAAAACAAGT ATATAGAGCT CGTCAACAGC AGAATCACTC TGGTCTGTCT 
ACCTTGTCTA AGAACATATA TACTTTGATT GATCTCCCAG CTGTTAATAC GTTAGAAGAG 
GAATATCCCA GGATTGAAAG TGAATCTACA GTAAATATTG GAGTTGGAGA ACCTGACTTT 
ATCTTGGGAC AGCTGTATCA CTTGTCTTCA GTACAGGCAC CTGAAGTGGC CAAGTCTTGG 
GCAGCCCTGG CTAGTTGGGC TTATAGATGG GGCCGGAAAG TAGTTGATAA TGCCAGTCAG 
GGAGAAGGTG TTCGTCTGCT GCCCCGAGAG AAATCTGAGG TTCAGAGCTT GCTTCCAGAC 
AATGTTACAG AGGAAGACAA AGAGAAGATT TATGGGATCC TTGGGCAAGC AGTGTGTCGT 
CCAGCTGGGA TTCAAGATGA AGATATGACT CTGCAAATCA CTGAAAATGA AGACAATGAA 
GAAGATGATA TGGTGGATGT TATATGGCGT CAGTTATTAA CAACTTGTCC CTGGCTTTCA 
GATCTTGATG AAAATGCAAC AGAAGGTTTA ATTAAAGTAT GGCGGAAAGT TGTAGACAGA 
ATCTTCAGTC TCTATAAACT CTCCTGCAGT GCATATTTCA CTTTCCTCAA ACTCAATGCT 
GGTCAGGTTC CACTTGATGA GGATGATCCA AGGCTGCATT TAAGAAATAC TTCTGAGCAA 
AGCACTGATG ATGTTATTGT GATGGCAACC CTAAGACTGT TAAGGCTGCT TGTTAAACAT 
GCTGGAGAGC TTAGACAGTA TCTAGAGCAT GGGCTAGAAA CTACACCTAC TGCTCCTTGG 
AGAGGTATTA TTCCTCAGCT CTTCTCTCGC CTCAATCATC CAGAAGTGTA TGTTCGTCAG 
AGTATTTGCA ACTTACTGTG TCGAGTGGCT CAAGATTCTC CTCATCTCAT ACTGTATCCT 
GCAATAGTGG GTACTATTTC ACTCAGCAGT GAATCCCAAA CCTCAGGGAA CAAGCTTCCT 
TCAGCGATTC CCACTTTGCT AGGTAATATT CAAGGAGAAG AACTGTTGGG TGGTGAATGT 
GATGGTGGGA GTCCACCAGC TTCTCAAGAA AGCACTAAAG ATGACACAAA AGTTGGATTA 
AATGAAGATC AAGCAATGAT GCAAGATTGT TACAGCAAAA TTGTGGAGAA ACTCTCAACT 
GCAAATCCAA CAATGGTATT GCAGGTTCAG ATGCTAGTGG CTGAGCTGCG CAGAGTTACA 
GTTCTGTGGG ATGAACTTTG GTTGGGCGTT TTACTGCAGC AGCACATGTA TGTCCTCAGA 
AGGATTCAGC AACTGGAAGA TGAAGTCAAA AGGGTCCAAA ACAACAACAC TCTACGCAAA 
GAGGAGAAGA TAGCAATCAT GCGTGAGAAG CATACAGCTC TAATGAAGCC CATTGTATTT 
GCTTTGGAAC ATGTACGGAG CATCACTGCA GCTCCTACAG AAACTCCTCA TGAGAAATGG 
TTTCAGGATA ACTATGGCGG TGCAATCGAA AATGCACTAG AGAAACTTAA GAATCCTTTG 
AATCCTGCTA AGCCTGGAAG CAGCTGGCTC CCCTTTAAAG AGGTTATGCT AAGTTTGCAA 
CAGAGAGCAC AGAAACGGGC TAGTTATCTT TTACGGCTTG AAGAAATCAG TCCTTGGTTG 
GCTGCCATGA TGAACACTGA AATAGCGCTC CCTGGAGAAG TATCCACCAG AGACACTGTC 
ACAATTCATA GTGTGGGCAG CACCATCACA ATTCTGCCTA CCAAAACCAA ACCTAAAAAG 
CTGCTTTTCC TGGGCTCAGA TGGGAAGAAC TATCCATACT TGTTCAAAGG TTTAGAGGAC 
TTGCACCTAG ATGAAAGAAT CATGCAATTC CTATCAATTG TGAACACAAT GTTTGCTACC 
ATTAACCGTC AAGAGACACC AAGGTTTCAT GCACGGCACT ACTCTGTGAC ACCTCTGGGA 
ACAAGATCAG GCCTGATCCA GTGGGTGGAT GGAGCTACAC CTTTGTTTGG TCTTTACAAA 
CGATGGCAAC AGCGGGAAGC TGCTTTACAA GCACAAAAGG CTCAGGATTC TTATCAGACT 
CCTCAGAATC CTGGAATCGT GCCTAGACCT AGTGAACTGT ACTACAGTAA AATTGGACCT 
GCTTTAAAGG CAGTTGGGCT TAGTCTTGAT GTGTCGCGTC GTGACTGGCC CTTGAATGTG 
ATGAGAGCTG TTCTAGAAGA ACTGATGGAA GCAACTCCCC CAAATCTCCT AGCTAAGGAG 
CTCTGGTCGT CATGTACTAC ACCAGATGAA TGGTGGAGAG TTACACAGTC TTATGCAAGA 
TCCACTGCAG TTATGTCCAT GGTTGGCTAC ATCATTGGTC TTGGAGACAG ACATCTGGAT 
AATGTTCTTA TAGATATGAC TACAGGAGAA GTTGTCCACA TAGATTATAA TGTTTGCTTT 
GAAAAAGGGA AGAGCCTTAG GGTACCAGAG AAGGTTCCAT TCCGAATGAC ACACAATATT 
GAAACAGCAC TTGGAGTGAC AGGAGTAGAG GGGGTTTTCA GGCTTTCTTG TGAACAGGTC 
CTTCATATCA TGCGGCGTGG GAGAGAAACT TTGCTGACGT TGCTTGAAGC TTTTGTTTAT 
GATCCTCTGG TGGACTGGAC AGCAGGGGGT GAGGCAGGAT TTGCAGGAGC TGTGTATGGT 
GGTGGCGGCC AGCAGGCTGA AAACAAGCAA AGCAAAAGAG AAATGGAAAG AGATATTACA 
CGTAGTCTCT TCTCTTCAAG AGTGGCTGAG ATAAAGGTTA ACTGGTTTAA GAACAGAGAT 
GAAATGTTGG CTGTGCTTCC AAAGTTGGAC AGTAGTTTAG AAGAATATCT CAACCTGCAA 
GAACAGTTGA CAGATGTAGA AAAATTGCAA GGAAAGCTAC TGGAGGAGAT AGAATTTCTT 
GAAGGTGCAG AAGGAGTGGA TCATCCCTCT CACACACTTC AGCACAGGTA TTCTGAGCAC 
ACACAGTTGC AGACTCAACA AAGAGCTGTC CAGGAGGCGA TCCAGGTGAA GCTGAATGAA 
TTTGAGCAGT GGATAACACA CTACCAAGCT GCTTTCAGTA ATTTAGAGGC AACACAACTT 
GCAAGTCTCC TTCAAGAAAT TAGCACACAA ATGGACCTAG GTCCTCCGAG TTATGTCCCA 
GCAACAGCAT TTCTGCAAAA TGCAGGCCAG GCACATCTCA TCAGTCAGTG TGAACAATTA 
GAAGGGGAGG TTGGTGCTCT TCTCCAGCAG AGAAGATCTG TTTTACGTGG TTGCCTCGAA 
CAGCTGCACA ACTATGCAAC AGTGGCTCTT CAGTATCCGA AAGCTGTGTT TCAGAAGCAC 
CGAATAGAGC AGTGGAAAAC TTGGATGGAA GAACTCATCT GCAACATGAC AATAGAGCGC 
TGTCAGGATA TCTTTAGGAA GTATGAAATG CAGTATGCTC CGCAGCCACC TCCAAGTGTG 
TGCCAGTTCA TAACCGCGAC GGAAATGACT CTCCAGCGCT ATGCAGCAGA TATAAATAGC 
AGACTTATCA GGCAGGTGGA GCGTCTGAAG CAGGAAGCAG TTACTGTACC TGTTTGTGAA 
GAGCAGCTGA AAGAAATCGA ACGCTGCATA AAAGTTTTCC TTCATGAAAA TGGAGAGGAG 
GGCTCACTGA GCTTAGCAAG TGTCATCATC TCTGCCCTTT GTACGCTGAC GAGGCGGAAT 
CTGATGATGG AAGGTGCAGC ATCCAGTGCT GGAGAGCAGC TGGTTGATCT GACTTCGAGA 
GATGGAGCAT GGTTTTTGGA GGAGCTCTGC AGCATGAGTG GGAATGTTAC TTGCCTTGTG 
CAGTTGCTGA AGCAGTGTCA TCTTGTACCC CAGGACTTAG ATATTCCTAA CCCAATTGAA 
GCATCAGAAG CTGTTCATTT AGCTAATGGA GTATACATCT CACTACAGGA ACTGAATTCC 
AACTTCCGGC AGATTATTTT CCCTGAAGCA CTTCGGTGCT TAATGAAAGG AGAATACACT 
TTAGAAAGTA TGCTCCATGA ATTAGATAAC CTTATTGAAC AAACAACTGA TGGAGTTTCT 
TTGCAAACAT TGGTTGAATC TCTTCAGGCC TACTTAAGAA ATGCAGCTAT GGGACTAGAG 
GAAGAAACAC ACACTCATTA CATTGATGTT GCAAGAGTAC TTCATGCTCA GTATGGCGAG 
CTGATTCAGC CAAGGAATGG TTCTGTGGAT GAAACTCCCA AAATGTCAGC TGGTCAGATG 
CTTTTGGTAG CCTTTGATGG AATGTTTGCT CAGGTGGAAA CTGCGTTTGG TTTATTGATT 
GAAAAGCTAA ACAAGATGGA AGTCCCTGTT GCTTGGCGTA AGATCGACAT CATTAGGGAA 
GCCCGCAGCA CTCAAGTTAA CTTCTTTGAT GATGACAATA ATCGCCAAGT TCTGGAGGAA 
ATCTTCTTTT TGAAGAGACT GCAAACAATT AAAGAATTTT TCAGACTCTG TGGTACCTTT 
TCTAAAACAT TGTCAGGAAT AAGTTCACTT GATGACCAGA ATGCAGTAAA CGGACCTGTT 
CAGATTGTCA ATGTGAAAGC CCTTTACAGA AACTCTTGCT TCAGTGAAGA CCAAATGGCC 
AAACCTATCA AGGCTTTTAC CGCTGACTTT GTGAGGCAGC TTTTAATTGG CCTTCCAAAT 
CAGGCTTTAG GACTGACTTT GTGCAGTTTC ATCAGCGCTT TGGGCGTGGA TATCATAGCT 
CAGGTAGAAG CCAAAGACTT TGGAGCAGAA AGCAAAGTTT CTGTGGATGA CCTGTGCAAG 
AAAGCTGTGG AGCACAATAT CCAGATTGGC AAGTTCTCAC AGCTGGTCAT GAACAGGGCA 
ACAGTGCTAG CTAGCTCGTA TGATACAGCG TGGAAGAAGC ATGATCTGGT GCGCAGACTC 
GAAACCAGTA TTTCTTCTTG CAAGACCAGT CTTCAGAGAG TGCAGCTGCA TATTGCCATG 
TTCCAATGGC AGCATGAAGA TCTCCTTATC AGTCGCCCAC AAGCCATTTC TGTTACTCCT 
CCACCTCGTT CAGCAATTTT AGCCAACATG AAGAAGAAAC TTCATACGCT CAGCCAGATT 
GAAGCTTCAA TTGCAACAGT TCAGGAGAAA CTAGCAGCAC TTGAAGCAAG CATTGAACAG 
CGTTTGAAAT GGGCAGGAGG AGCTAATCCT GCACTGGCAC CAGTCTTGCA AGATTTCGAT 
GCCACTATTG CTGAAAGAAG AAGCCTTGTC CTGAAAGAAA GTCAAAGAGC AAGTCAGGTT 
ACTTTCCTGT GCAGTAATAT CATCCACTTT GAAAGTTTAC GTACTAGAAC AGCAGAAGCC 
TTAAATCTTG ATGCTGCATT ATTTGAACTT CTCAAACGCT GTCAACAAAT GTGTTCATTT 
GCATCACAGT TCAACAGCTC AGTGTCTGAA TTGGAGCTTC GTCTGCTGCA GAGAGTGGGC 
ACTGGTCTTG AACATCCTAT TGGCAGCTCT GAGTGGCTTC ACTCAGCCCA CAAGCAGTTG 
ACACAGGAAA TTACTACACA GAGAACTGTA CAGGCAGAAA GAGAACAGCA AATAGAAACA 
GTTTGTGAAA CTATTCAAAA TCTGGTGGAT AATATTAAAA CTGTGCTCAC AGGTCATAAT 
AGACAACTTG GAGATGTCAA GCATCTACTG AAGGCTATGG CAAAGGATGA AGAAGCAGCT 
TTGGCAGATG GTGAAGATGT TCCCTATGAA AATAGTGTTA GGCAGTTCTT GGGCGAATAT 
AAATCATGGC AAGATAATAT TCAGACAGTT TTGTTTACAT TAGTTCAGGT TATGGGTCAA 
GTCCGCAATC AGGAACATGT TGAAATGCTG CAGGAAATAA CCCCCACCCT GAAAGAGCTA 
AAAACACAGA GCCAAAGTGT CTACAATAAT CTAGTGAGTT TTGCATCACC CTTAGTCACG 
GATACATCAA ATGAATGCTC AAGCCCAACA TCAGCTGCTA CATACCAGCC AACCTTTGCT 
GCAGCTGTAC GTAGCAATAC AGGGCAGAAA ACTCAGCCAG AGGTGATGTC ACAGAATGCA 
AGAAAGCTAA TTCAGAAAAA TCTTGCCACA TCAGCAGATA CTCCTCCAAG CACAGTCCCA 
GGAACTGGCA AAAGTGTTGC CTGTAGTCCC AAGAAGACAG CCAGGGACCC TAAAACAGGA 
AAAGCTGTGC AGGAAAGGAA TTCATATGCA GTTAGTGTGT GGAAACGAGT CAAAGCCAAG 
TTGGAGGGCC GAGATGTGGA TCCCAGCAGG AGAATGTCTG TTGCTGAACA GGTTGACTTT 
GTGATTAAGG AAGCAACTAA TCTAGATAAC TTGGCTCAGC TGTATGAAGG TTGGACTGCC 
TGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_414907.4
CDS	176..11164
Translation	MSRRAPGSRLSGTGSRQHYHPRGWNDWQPRTDSASADQDNLKYSSSRDRGNSASYGLQPSNSAVVSRQRHDDIRVHTDIQNEDKGGYSVNGGSGENTYGRKSLGQELRVNNVTNPDFTSVPHGNRALATKDMRKSQERSLSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILTAIHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSLSTKDEVKLLYLCATYKALETVGEKKAFSSLMQLVMTSLQSILENVDTPELLCKCVKCILLLSRCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLTFSTTLLGQFLEDMEAYAEDLSHVASGESLDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQMENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPESKLLVLRYHKEKEVVAAAHAVYQAVLSLKNIPVLETAYRLILGEMTCALNSLLYSLHLPEACSEIQHDSFKKHVFTVENAKFVVIFDLSALSTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDIAVHFPAVQYAVLYTLYSHCSRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGLLLKKDNLTQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLLEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGARVRQTFGKLLKSIPLDVVLSNRNHTEIQEISLAIRSHMSKAPSNTFHPQDFSDVISFILYGSTHRTGKDNWLERLFYSCQRLDKRDQPTIPRNLLKTDAVLWQWAVWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNNSLSQGNELEVTIMMLVEALCELHCPEAIQGIAVWSSAVVGKSLTWINSVAQQAEGRFEKATAEYQEHLCAMTGVDCCISGFDKTVLKLANSNSVNNASPKHSLNGETRKTVLTKPSDCSPEVINYLGNKACECYISIADWSAVQEWQNMVHDLKKSTSNTSINLKADFNYIKSLSSFESGELTECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKAVEVQLLRSSVCLATAVNHMEQEQKWQSISENVIKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLTSEDCLIPLFNDALRSCKQHDVRPWMHALRYTVYQNQLMGKLKEPTVPIKSHLMELGLTAAKFARKRGNIALATRLLAQCSEVQLGKTTTAQDLVQHFKKLSAPGQIDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRARQQQNHSGLSTLSKNIYTLIDLPAVNTLEEEYPRIESESTVNIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQSLLPDNVTEEDKEKIYGILGQAVCRPAGIQDEDMTLQITENEDNEEDDMVDVIWRQLLTTCPWLSDLDENATEGLIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVPLDEDDPRLHLRNTSEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQTSGNKLPSAIPTLLGNIQGEELLGGECDGGSPPASQESTKDDTKVGLNEDQAMMQDCYSKIVEKLSTANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPTETPHEKWFQDNYGGAIENALEKLKNPLNPAKPGSSWLPFKEVMLSLQQRAQKRASYLLRLEEISPWLAAMMNTEIALPGEVSTRDTVTIHSVGSTITILPTKTKPKKLLFLGSDGKNYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLNVMRAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLAVLPKLDSSLEEYLNLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFSNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHNYATVALQYPKAVFQKHRIEQWKTWMEELICNMTIERCQDIFRKYEMQYAPQPPPSVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEEQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPIEASEAVHLANGVYISLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDNLIEQTTDGVSLQTLVESLQAYLRNAAMGLEEETHTHYIDVARVLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLIEKLNKMEVPVAWRKIDIIREARSTQVNFFDDDNNRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGISSLDDQNAVNGPVQIVNVKALYRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLISRPQAISVTPPPRSAILANMKKKLHTLSQIEASIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFDATIAERRSLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELLKRCQQMCSFASQFNSSVSELELRLLQRVGTGLEHPIGSSEWLHSAHKQLTQEITTQRTVQAEREQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQVMGQVRNQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDTSNECSSPTSAATYQPTFAAAVRSNTGQKTQPEVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKTARDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPSRRMSVAEQVDFVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_414907.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_414907.6

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCGGCACCG GCAGCAGGCA GCACTACCAC 
CCGCGGGGCT GGAATGACTG GCAACCCAGA ACTGACAGTG CATCAGCTGA CCAGGACAAT 
TTAAAATACT CTTCATCCAG AGATCGGGGC AATTCTGCCT CCTATGGATT ACAACCCTCA 
AATTCAGCTG TGGTGTCTCG ACAAAGACAC GATGATATCA GAGTCCACAC TGACATACAG 
AATGAAGACA AAGGTGGCTA CAGTGTCAAT GGAGGATCTG GGGAAAATAC TTATGGTCGG 
AAGTCGTTGG GGCAAGAGCT GAGAGTTAAC AATGTGACCA ATCCTGATTT TACCAGTGTT 
CCGCATGGGA ATCGTGCTTT AGCCACAAAA GACATGAGGA AATCACAGGA GCGATCGTTG 
TCTTATTCTG ACGAGTCTCG ACTGTCAAAT CTTCTTCGGA GGATTACTCG GGAAGACGAC 
AGAGACCGAA GACTGGCTAC TGTAAAGCAG CTGAAAGAAT TCATTCAGCA ACCAGAAAAC 
AAACTGGTAC TAGTTAAACA ACTGGATAAT ATCCTGACTG CCATTCATGA TGTACTCAAT 
GAAAGTAGCA AATTGCTTCA AGAACTGAGA CAGGAAGGAG CTTGCTGTCT TGGCCTTCTT 
TGTGCTTCTC TAAGCTATGA GGCTGAGAAG ATATTTAAAT GGATTTTTAG CAAATTCAGC 
TTATCCACAA AAGATGAAGT TAAACTTCTT TATTTGTGTG CAACCTACAA AGCACTGGAG 
ACTGTAGGAG AAAAGAAAGC CTTTTCATCT TTAATGCAGC TTGTAATGAC CAGCCTACAG 
TCCATTCTAG AAAATGTGGA TACACCAGAG TTACTGTGCA AATGTGTCAA GTGCATTCTT 
CTGTTGTCCC GATGTTATCC GCACATTTTC AGCACCAATT TTCGGGACAC AGTGGACATA 
CTGGTTGGGT GGCATATAGA TCACACTCAG AAGCCTTCAT TGACTCAGCA GGTGTCCGGG 
TGGCTGCAGA GCTTGGAGCC CTTTTGGGTG GCTGATCTCA CTTTTTCTAC CACACTCTTG 
GGTCAATTTT TAGAAGATAT GGAAGCTTAT GCTGAGGACC TCAGCCATGT GGCTTCTGGG 
GAGTCCCTAG ATGAAGATAT TCCTCCTCCC TCTGTATCAC TACCTAAACT GGCTGCGCTT 
CTTCGAGTTT TCAGTACTGT GGTGAGAAGT ATTGGGGAGC GCTTCAGCCC AATTAGAGGA 
CCACCGATTA CAGAAGCATA TGTAACTGAT GTTCTGTACA GAGTAATGAG ATGTGTGACT 
GCAGCAAACC AAGTATTCTT TTCAGAAGCA GTACTCACAG CTGCCAATGA GTGTGTTGGT 
GTTTTACTTG GCAGTCTGGA TCCAAGCATG ACCATTCACT GTGACATGGT TATCACCTAC 
GGATTGGATC AAATGGAAAA TTGTCAGACT TGTGGCACTG ATTATATCAT TTCAGTCCTG 
AATCTGTTGA CGCTGATTGT TGAACAAATA AATACAAAAC TACCATCGTC ATTTGTAGAG 
AAATTATTTA TTCCAGAGTC TAAACTGCTT GTACTTCGTT ATCATAAGGA AAAAGAGGTT 
GTTGCAGCAG CCCACGCTGT GTATCAGGCA GTACTGAGCC TGAAGAACAT TCCTGTTTTG 
GAGACTGCTT ACAGGTTGAT TCTAGGAGAA ATGACCTGTG CTCTAAACAG TCTTCTTTAT 
AGTTTACATC TTCCCGAGGC CTGTTCTGAA ATCCAGCATG ACTCTTTCAA GAAGCATGTA 
TTCACTGTGG AAAATGCAAA GTTTGTTGTT ATATTTGACC TCAGTGCCCT AAGTACTATT 
GGAAATGCTA AGAACTCATT AATTGGGATG TGGGCCCTTT CGCCAACTGT GTTTGCACTA 
CTGAGTAAAA ATCTGATGAT TGTTCATGGT GACATAGCAG TTCATTTTCC TGCTGTCCAG 
TATGCAGTAC TCTATACGTT GTATTCGCAT TGTTCCAGAC ATGACCATTT TATATCCAGT 
AGCCTCAGTT CTTCCTCTCC TTCCCTGTTC GATGGTGCAG TTATTAGTAC TGTAACCACA 
GCAACAAAAA AACATTTCTC AATCATACTG AACCTTTTGG GGCTACTGTT GAAGAAGGAT 
AACCTTACTC AAGATACCAG GAAACTGCTT ATGACGTGGG CTTTGGAAGT GGCTGTTCTG 
ATGAAGAAAT CAGAGACATA TGCACCCTTA TTTTCTCTCC CATCTTTCCA TAAGTTTTGC 
AAAGGACTTC TAGCAAACAC CCTCCTGGAA GATGTGAATA TTTGTCTTCA AGCGTGTAGC 
AGCCTCCATG CCCTGTCCTC CTCCCTACCA GATGACCTTT TACAGAGATG TGTTGATGTG 
TGTCGTGTTC AACTAGTTCA TAGCGGTGCT CGTGTAAGGC AGACTTTCGG AAAACTTCTG 
AAGTCAATTC CCTTAGATGT GGTGTTGAGC AACCGTAACC ACACAGAAAT ACAAGAAATT 
TCTTTGGCTA TAAGAAGTCA TATGAGCAAA GCTCCAAGTA ATACATTCCA CCCACAGGAT 
TTCTCTGATG TCATTAGTTT TATTTTGTAT GGAAGCACTC ATCGAACTGG GAAAGATAAT 
TGGTTGGAAA GGTTATTCTA TAGCTGTCAA AGGCTGGATA AACGAGATCA ACCAACCATC 
CCTCGTAATC TGTTGAAGAC TGATGCTGTT CTCTGGCAAT GGGCTGTTTG GGAAGCAGCT 
CAGTTTACTG TTCTTTCTAA GCTGAGAACT CCTCTGGGTA GAGCCCAAGA TACATTTCAA 
ACAATTGAAG GTATTATTAG GAGTCTTGCA GCGCATACGC TAAACCCTGA TCAAGATGTT 
AGCCAGTGGA CTACTGCAGA CAATGATGAG GGACACAGCA GCAATCAACT TAGGCTTGTT 
CTCCTTCTAC AGTATCTGGA GAACTTGGAA AAACTGATGT ACAATGCTTA TGAAGGATGT 
GCCAATGCCC TTACCTCTCC ACCCAAGGTT ATCAGGACAT TCTTTTATAC TAATCGCCAG 
ACGTGCCAAG ATTGGCTGAC GCGGATTAGA CTTTCCATCA TGAGAGTTGG ACTGCTGGCC 
GGCCAGCCTG CTGTGACAGT CAGACATGGT TTTGACTTAC TCACAGAAAT GAAAACTAAT 
AACAGTTTAT CTCAGGGAAA TGAATTGGAA GTGACAATTA TGATGCTGGT GGAAGCATTA 
TGTGAACTTC ACTGCCCTGA AGCTATTCAG GGTATTGCTG TCTGGTCTTC TGCTGTAGTC 
GGGAAGAGTC TTACCTGGAT CAACTCTGTA GCTCAACAGG CAGAAGGGCG GTTTGAAAAA 
GCAACTGCGG AGTACCAGGA GCACCTGTGT GCCATGACAG GAGTAGATTG CTGTATATCG 
GGATTTGACA AAACTGTTCT GAAGTTGGCC AATTCCAACA GTGTGAATAA CGCCAGCCCA 
AAGCACTCCT TGAACGGAGA AACTAGAAAA ACTGTTCTGA CTAAGCCAAG TGACTGTTCA 
CCTGAAGTTA TAAATTACTT GGGTAACAAG GCATGTGAAT GTTACATTTC AATTGCTGAT 
TGGTCTGCTG TTCAGGAATG GCAAAATATG GTCCATGACT TGAAGAAAAG CACGAGTAAT 
ACCTCAATCA ATCTGAAAGC AGATTTTAAC TACATCAAAT CCCTGAGCAG CTTTGAGTCT 
GGTGAACTTA CTGAATGCAC TGAACAACTG GAATTATTAC CAGGAGAAAA TATCAACCTA 
CTTGCAGGGG GATCCAAAGA AAAAATAGAC ATGAAGAAGC TCCTTCCTAA TATGCTAAGT 
CCTGACCCGA GAGAACTTCA AAAAGCAGTT GAAGTACAAC TTCTGAGAAG TTCGGTGTGT 
CTGGCAACAG CTGTAAACCA CATGGAGCAG GAGCAAAAGT GGCAGTCAAT ATCTGAAAAT 
GTTATAAAAT ACTTAAAGCA AACATCACGA ATAGCTATTG GGCCTTTAAG ACTTTCCACG 
CTCACCGTGT CTCAGTCTTT ACCAGTGTTG AGCACTCTTC AGTTGTATTG TTCTTCTGCT 
TTAGAAAACA CCGTATCCAA CAGGCTGACA TCAGAGGACT GTCTTATACC TCTCTTCAAT 
GATGCTCTGA GGTCATGTAA ACAGCATGAT GTGAGGCCGT GGATGCATGC ACTGAGATAC 
ACGGTGTATC AGAATCAACT GATGGGAAAG CTTAAAGAAC CAACAGTCCC AATTAAGAGT 
CATCTAATGG AGCTGGGCTT AACAGCAGCA AAATTTGCAC GGAAGCGAGG AAATATTGCC 
CTGGCTACAC GGTTGCTTGC CCAGTGCAGT GAAGTTCAGC TAGGAAAAAC CACCACTGCA 
CAGGACTTAG TCCAGCACTT TAAGAAACTG TCTGCACCAG GTCAGATTGA TGAAAAGTGG 
GGTCCTGAAC TTGATATTGA GAAAACAAAG CTGTTATACA CAGCAGGTCA GTCAACACAT 
GCCATGGAAA TGCTGAGTTC TTGTGCTATA TCATTTTGCA AATCTGCCAA GGCTGAATAT 
GCAGTTGCTA AATCTATTCT TACGCTGGCT AAATGGATTC AAGCAGAATG GAAAGAGATT 
TCAGGACAGT TAAAACAAGT ATATAGAGCT CGTCAACAGC AGAATCACTC TGGTCTGTCT 
ACCTTGTCTA AGAACATATA TACTTTGATT GATCTCCCAG CTGTTAATAC GTTAGAAGAG 
GAATATCCCA GGATTGAAAG TGAATCTACA GTAAATATTG GAGTTGGAGA ACCTGACTTT 
ATCTTGGGAC AGCTGTATCA CTTGTCTTCA GTACAGGCAC CTGAAGTGGC CAAGTCTTGG 
GCAGCCCTGG CTAGTTGGGC TTATAGATGG GGCCGGAAAG TAGTTGATAA TGCCAGTCAG 
GGAGAAGGTG TTCGTCTGCT GCCCCGAGAG AAATCTGAGG TTCAGAGCTT GCTTCCAGAC 
AATGTTACAG AGGAAGACAA AGAGAAGATT TATGGGATCC TTGGGCAAGC AGTGTGTCGT 
CCAGCTGGGA TTCAAGATGA AGATATGACT CTGCAAATCA CTGAAAATGA AGACAATGAA 
GAAGATGATA TGGTGGATGT TATATGGCGT CAGTTATTAA CAACTTGTCC CTGGCTTTCA 
GATCTTGATG AAAATGCAAC AGAAGGTTTA ATTAAAGTAT GGCGGAAAGT TGTAGACAGA 
ATCTTCAGTC TCTATAAACT CTCCTGCAGT GCATATTTCA CTTTCCTCAA ACTCAATGCT 
GGTCAGGTTC CACTTGATGA GGATGATCCA AGGCTGCATT TAAGAAATAC TTCTGAGCAA 
AGCACTGATG ATGTTATTGT GATGGCAACC CTAAGACTGT TAAGGCTGCT TGTTAAACAT 
GCTGGAGAGC TTAGACAGTA TCTAGAGCAT GGGCTAGAAA CTACACCTAC TGCTCCTTGG 
AGAGGTATTA TTCCTCAGCT CTTCTCTCGC CTCAATCATC CAGAAGTGTA TGTTCGTCAG 
AGTATTTGCA ACTTACTGTG TCGAGTGGCT CAAGATTCTC CTCATCTCAT ACTGTATCCT 
GCAATAGTGG GTACTATTTC ACTCAGCAGT GAATCCCAAA CCTCAGGGAA CAAGCTTCCT 
TCAGCGATTC CCACTTTGCT AGGTAATATT CAAGGAGAAG AACTGTTGGG TGGTGAATGT 
GATGGTGGGA GTCCACCAGC TTCTCAAGAA AGCACTAAAG ATGACACAAA AGTTGGATTA 
AATGAAGATC AAGCAATGAT GCAAGATTGT TACAGCAAAA TTGTGGAGAA ACTCTCAACT 
GCAAATCCAA CAATGGTATT GCAGGTTCAG ATGCTAGTGG CTGAGCTGCG CAGAGTTACA 
GTTCTGTGGG ATGAACTTTG GTTGGGCGTT TTACTGCAGC AGCACATGTA TGTCCTCAGA 
AGGATTCAGC AACTGGAAGA TGAAGTCAAA AGGGTCCAAA ACAACAACAC TCTACGCAAA 
GAGGAGAAGA TAGCAATCAT GCGTGAGAAG CATACAGCTC TAATGAAGCC CATTGTATTT 
GCTTTGGAAC ATGTACGGAG CATCACTGCA GCTCCTACAG AAACTCCTCA TGAGAAATGG 
TTTCAGGATA ACTATGGCGG TGCAATCGAA AATGCACTAG AGAAACTTAA GAATCCTTTG 
AATCCTGCTA AGCCTGGAAG CAGCTGGCTC CCCTTTAAAG AGGTTATGCT AAGTTTGCAA 
CAGAGAGCAC AGAAACGGGC TAGTTATCTT TTACGGCTTG AAGAAATCAG TCCTTGGTTG 
GCTGCCATGA TGAACACTGA AATAGCGCTC CCTGGAGAAG TATCCACCAG AGACACTGTC 
ACAATTCATA GTGTGGGCAG CACCATCACA ATTCTGCCTA CCAAAACCAA ACCTAAAAAG 
CTGCTTTTCC TGGGCTCAGA TGGGAAGAAC TATCCATACT TGTTCAAAGG TTTAGAGGAC 
TTGCACCTAG ATGAAAGAAT CATGCAATTC CTATCAATTG TGAACACAAT GTTTGCTACC 
ATTAACCGTC AAGAGACACC AAGGTTTCAT GCACGGCACT ACTCTGTGAC ACCTCTGGGA 
ACAAGATCAG GCCTGATCCA GTGGGTGGAT GGAGCTACAC CTTTGTTTGG TCTTTACAAA 
CGATGGCAAC AGCGGGAAGC TGCTTTACAA GCACAAAAGG CTCAGGATTC TTATCAGACT 
CCTCAGAATC CTGGAATCGT GCCTAGACCT AGTGAACTGT ACTACAGTAA AATTGGACCT 
GCTTTAAAGG CAGTTGGGCT TAGTCTTGAT GTGTCGCGTC GTGACTGGCC CTTGAATGTG 
ATGAGAGCTG TTCTAGAAGA ACTGATGGAA GCAACTCCCC CAAATCTCCT AGCTAAGGAG 
CTCTGGTCGT CATGTACTAC ACCAGATGAA TGGTGGAGAG TTACACAGTC TTATGCAAGA 
TCCACTGCAG TTATGTCCAT GGTTGGCTAC ATCATTGGTC TTGGAGACAG ACATCTGGAT 
AATGTTCTTA TAGATATGAC TACAGGAGAA GTTGTCCACA TAGATTATAA TGTTTGCTTT 
GAAAAAGGGA AGAGCCTTAG GGTACCAGAG AAGGTTCCAT TCCGAATGAC ACACAATATT 
GAAACAGCAC TTGGAGTGAC AGGAGTAGAG GGGGTTTTCA GGCTTTCTTG TGAACAGGTC 
CTTCATATCA TGCGGCGTGG GAGAGAAACT TTGCTGACGT TGCTTGAAGC TTTTGTTTAT 
GATCCTCTGG TGGACTGGAC AGCAGGGGGT GAGGCAGGAT TTGCAGGAGC TGTGTATGGT 
GGTGGCGGCC AGCAGGCTGA AAACAAGCAA AGCAAAAGAG AAATGGAAAG AGATATTACA 
CGTAGTCTCT TCTCTTCAAG AGTGGCTGAG ATAAAGGTTA ACTGGTTTAA GAACAGAGAT 
GAAATGTTGG CTGTGCTTCC AAAGTTGGAC AGTAGTTTAG AAGAATATCT CAACCTGCAA 
GAACAGTTGA CAGATGTAGA AAAATTGCAA GGAAAGCTAC TGGAGGAGAT AGAATTTCTT 
GAAGGTGCAG AAGGAGTGGA TCATCCCTCT CACACACTTC AGCACAGGTA TTCTGAGCAC 
ACACAGTTGC AGACTCAACA AAGAGCTGTC CAGGAGGCGA TCCAGGTGAA GCTGAATGAA 
TTTGAGCAGT GGATAACACA CTACCAAGCT GCTTTCAGTA ATTTAGAGGC AACACAACTT 
GCAAGTCTCC TTCAAGAAAT TAGCACACAA ATGGACCTAG GTCCTCCGAG TTATGTCCCA 
GCAACAGCAT TTCTGCAAAA TGCAGGCCAG GCACATCTCA TCAGTCAGTG TGAACAATTA 
GAAGGGGAGG TTGGTGCTCT TCTCCAGCAG AGAAGATCTG TTTTACGTGG TTGCCTCGAA 
CAGCTGCACA ACTATGCAAC AGTGGCTCTT CAGTATCCGA AAGCTGTGTT TCAGAAGCAC 
CGAATAGAGC AGTGGAAAAC TTGGATGGAA GAACTCATCT GCAACATGAC AATAGAGCGC 
TGTCAGGATA TCTTTAGGAA GTATGAAATG CAGTATGCTC CGCAGCCACC TCCAAGTGTG 
TGCCAGTTCA TAACCGCGAC GGAAATGACT CTCCAGCGCT ATGCAGCAGA TATAAATAGC 
AGACTTATCA GGCAGGTGGA GCGTCTGAAG CAGGAAGCAG TTACTGTACC TGTTTGTGAA 
GAGCAGCTGA AAGAAATCGA ACGCTGCATA AAAGTTTTCC TTCATGAAAA TGGAGAGGAG 
GGCTCACTGA GCTTAGCAAG TGTCATCATC TCTGCCCTTT GTACGCTGAC GAGGCGGAAT 
CTGATGATGG AAGGTGCAGC ATCCAGTGCT GGAGAGCAGC TGGTTGATCT GACTTCGAGA 
GATGGAGCAT GGTTTTTGGA GGAGCTCTGC AGCATGAGTG GGAATGTTAC TTGCCTTGTG 
CAGTTGCTGA AGCAGTGTCA TCTTGTACCC CAGGACTTAG ATATTCCTAA CCCAATTGAA 
GCATCAGAAG CTGTTCATTT AGCTAATGGA GTATACATCT CACTACAGGA ACTGAATTCC 
AACTTCCGGC AGATTATTTT CCCTGAAGCA CTTCGGTGCT TAATGAAAGG AGAATACACT 
TTAGAAAGTA TGCTCCATGA ATTAGATAAC CTTATTGAAC AAACAACTGA TGGAGTTTCT 
TTGCAAACAT TGGTTGAATC TCTTCAGGCC TACTTAAGAA ATGCAGCTAT GGGACTAGAG 
GAAGAAACAC ACACTCATTA CATTGATGTT GCAAGAGTAC TTCATGCTCA GTATGGCGAG 
CTGATTCAGC CAAGGAATGG TTCTGTGGAT GAAACTCCCA AAATGTCAGC TGGTCAGATG 
CTTTTGGTAG CCTTTGATGG AATGTTTGCT CAGGTGGAAA CTGCGTTTGG TTTATTGATT 
GAAAAGCTAA ACAAGATGGA AGTCCCTGTT GCTTGGCGTA AGATCGACAT CATTAGGGAA 
GCCCGCAGCA CTCAAGTTAA CTTCTTTGAT GATGACAATA ATCGCCAAGT TCTGGAGGAA 
ATCTTCTTTT TGAAGAGACT GCAAACAATT AAAGAATTTT TCAGACTCTG TGGTACCTTT 
TCTAAAACAT TGTCAGGAAT AAGTTCACTT GATGACCAGA ATGCAGTAAA CGGACCTGTT 
CAGATTGTCA ATGTGAAAGC CCTTTACAGA AACTCTTGCT TCAGTGAAGA CCAAATGGCC 
AAACCTATCA AGGCTTTTAC CGCTGACTTT GTGAGGCAGC TTTTAATTGG CCTTCCAAAT 
CAGGCTTTAG GACTGACTTT GTGCAGTTTC ATCAGCGCTT TGGGCGTGGA TATCATAGCT 
CAGGTAGAAG CCAAAGACTT TGGAGCAGAA AGCAAAGTTT CTGTGGATGA CCTGTGCAAG 
AAAGCTGTGG AGCACAATAT CCAGATTGGC AAGTTCTCAC AGCTGGTCAT GAACAGGGCA 
ACAGTGCTAG CTAGCTCGTA TGATACAGCG TGGAAGAAGC ATGATCTGGT GCGCAGACTC 
GAAACCAGTA TTTCTTCTTG CAAGACCAGT CTTCAGAGAG TGCAGCTGCA TATTGCCATG 
TTCCAATGGC AGCATGAAGA TCTCCTTATC AGTCGCCCAC AAGCCATTTC TGTTACTCCT 
CCACCTCGTT CAGCAATTTT AGCCAACATG AAGAAGAAAC TTCATACGCT CAGCCAGATT 
GAAGCTTCAA TTGCAACAGT TCAGGAGAAA CTAGCAGCAC TTGAAGCAAG CATTGAACAG 
CGTTTGAAAT GGGCAGGAGG AGCTAATCCT GCACTGGCAC CAGTCTTGCA AGATTTCGAT 
GCCACTATTG CTGAAAGAAG AAGCCTTGTC CTGAAAGAAA GTCAAAGAGC AAGTCAGGTT 
ACTTTCCTGT GCAGTAATAT CATCCACTTT GAAAGTTTAC GTACTAGAAC AGCAGAAGCC 
TTAAATCTTG ATGCTGCATT ATTTGAACTT CTCAAACGCT GTCAACAAAT GTGTTCATTT 
GCATCACAGT TCAACAGCTC AGTGTCTGAA TTGGAGCTTC GTCTGCTGCA GAGAGTGGGC 
ACTGGTCTTG AACATCCTAT TGGCAGCTCT GAGTGGCTTC ACTCAGCCCA CAAGCAGTTG 
ACACAGGAAA TTACTACACA GAGAACTGTA CAGGCAGAAA GAGAACAGCA AATAGAAACA 
GTTTGTGAAA CTATTCAAAA TCTGGTGGAT AATATTAAAA CTGTGCTCAC AGGTCATAAT 
AGACAACTTG GAGATGTCAA GCATCTACTG AAGGCTATGG CAAAGGATGA AGAAGCAGCT 
TTGGCAGATG GTGAAGATGT TCCCTATGAA AATAGTGTTA GGCAGTTCTT GGGCGAATAT 
AAATCATGGC AAGATAATAT TCAGACAGTT TTGTTTACAT TAGTTCAGGT TATGGGTCAA 
GTCCGCAATC AGGAACATGT TGAAATGCTG CAGGAAATAA CCCCCACCCT GAAAGAGCTA 
AAAACACAGA GCCAAAGTGT CTACAATAAT CTAGTGAGTT TTGCATCACC CTTAGTCACG 
GATACATCAA ATGAATGCTC AAGCCCAACA TCAGCTGCTA CATACCAGCC AACCTTTGCT 
GCAGCTGTAC GTAGCAATAC AGGGCAGAAA ACTCAGCCAG AGGTGATGTC ACAGAATGCA 
AGAAAGCTAA TTCAGAAAAA TCTTGCCACA TCAGCAGATA CTCCTCCAAG CACAGTCCCA 
GGAACTGGCA AAAGTGTTGC CTGTAGTCCC AAGAAGACAG CCAGGGACCC TAAAACAGGA 
AAAGCTGTGC AGGAAAGGAA TTCATATGCA GTTAGTGTGT GGAAACGAGT CAAAGCCAAG 
TTGGAGGGCC GAGATGTGGA TCCCAGCAGG AGAATGTCTG TTGCTGAACA GGTTGACTTT 
GTGATTAAGG AAGCAACTAA TCTAGATAAC TTGGCTCAGC TGTATGAAGG TTGGACTGCC 
TGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RP11-345J4.5 ( XM_015294535.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150021.1 Gallus gallus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

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CloneID	OGa144592
Clone ID Related Accession (Same CDS sequence)	XM_015294535.2
Accession Version	XM_015294535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA ACCCAGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGAGTT AACAATGTGA CCAATCCTGA TTTTACCAGT 
GTTCCGCATG GGAATCGTGC TTTAGCCACA AAAGACATGA GGAAATCACA GGAGCGATCG 
TTGTCTTATT CTGACGAGTC TCGACTGTCA AATCTTCTTC GGAGGATTAC TCGGGAAGAC 
GACAGAGACC GAAGACTGGC TACTGTAAAG CAGCTGAAAG AATTCATTCA GCAACCAGAA 
AACAAACTGG TACTAGTTAA ACAACTGGAT AATATCCTGA CTGCCATTCA TGATGTACTC 
AATGAAAGTA GCAAATTGCT TCAAGAACTG AGACAGGAAG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTAAGCTA TGAGGCTGAG AAGATATTTA AATGGATTTT TAGCAAATTC 
AGCTTATCCA CAAAAGATGA AGTTAAACTT CTTTATTTGT GTGCAACCTA CAAAGCACTG 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTTTAATGC AGCTTGTAAT GACCAGCCTA 
CAGTCCATTC TAGAAAATGT GGATACACCA GAGTTACTGT GCAAATGTGT CAAGTGCATT 
CTTCTGTTGT CCCGATGTTA TCCGCACATT TTCAGCACCA ATTTTCGGGA CACAGTGGAC 
ATACTGGTTG GGTGGCATAT AGATCACACT CAGAAGCCTT CATTGACTCA GCAGGTGTCC 
GGGTGGCTGC AGAGCTTGGA GCCCTTTTGG GTGGCTGATC TCACTTTTTC TACCACACTC 
TTGGGTCAAT TTTTAGAAGA TATGGAAGCT TATGCTGAGG ACCTCAGCCA TGTGGCTTCT 
GGGGAGTCCC TAGATGAAGA TATTCCTCCT CCCTCTGTAT CACTACCTAA ACTGGCTGCG 
CTTCTTCGAG TTTTCAGTAC TGTGGTGAGA AGTATTGGGG AGCGCTTCAG CCCAATTAGA 
GGACCACCGA TTACAGAAGC ATATGTAACT GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACTGCAGCAA ACCAAGTATT CTTTTCAGAA GCAGTACTCA CAGCTGCCAA TGAGTGTGTT 
GGTGTTTTAC TTGGCAGTCT GGATCCAAGC ATGACCATTC ACTGTGACAT GGTTATCACC 
TACGGATTGG ATCAAATGGA AAATTGTCAG ACTTGTGGCA CTGATTATAT CATTTCAGTC 
CTGAATCTGT TGACGCTGAT TGTTGAACAA ATAAATACAA AACTACCATC GTCATTTGTA 
GAGAAATTAT TTATTCCAGA GTCTAAACTG CTTGTACTTC GTTATCATAA GGAAAAAGAG 
GTTGTTGCAG CAGCCCACGC TGTGTATCAG GCAGTACTGA GCCTGAAGAA CATTCCTGTT 
TTGGAGACTG CTTACAGGTT GATTCTAGGA GAAATGACCT GTGCTCTAAA CAGTCTTCTT 
TATAGTTTAC ATCTTCCCGA GGCCTGTTCT GAAATCCAGC ATGACTCTTT CAAGAAGCAT 
GTATTCACTG TGGAAAATGC AAAGTTTGTT GTTATATTTG ACCTCAGTGC CCTAAGTACT 
ATTGGAAATG CTAAGAACTC ATTAATTGGG ATGTGGGCCC TTTCGCCAAC TGTGTTTGCA 
CTACTGAGTA AAAATCTGAT GATTGTTCAT GGTGACATAG CAGTTCATTT TCCTGCTGTC 
CAGTATGCAG TACTCTATAC GTTGTATTCG CATTGTTCCA GACATGACCA TTTTATATCC 
AGTAGCCTCA GTTCTTCCTC TCCTTCCCTG TTCGATGGTG CAGTTATTAG TACTGTAACC 
ACAGCAACAA AAAAACATTT CTCAATCATA CTGAACCTTT TGGGGCTACT GTTGAAGAAG 
GATAACCTTA CTCAAGATAC CAGGAAACTG CTTATGACGT GGGCTTTGGA AGTGGCTGTT 
CTGATGAAGA AATCAGAGAC ATATGCACCC TTATTTTCTC TCCCATCTTT CCATAAGTTT 
TGCAAAGGAC TTCTAGCAAA CACCCTCCTG GAAGATGTGA ATATTTGTCT TCAAGCGTGT 
AGCAGCCTCC ATGCCCTGTC CTCCTCCCTA CCAGATGACC TTTTACAGAG ATGTGTTGAT 
GTGTGTCGTG TTCAACTAGT TCATAGCGGT GCTCGTGTAA GGCAGACTTT CGGAAAACTT 
CTGAAGTCAA TTCCCTTAGA TGTGGTGTTG AGCAACCGTA ACCACACAGA AATACAAGAA 
ATTTCTTTGG CTATAAGAAG TCATATGAGC AAAGCTCCAA GTAATACATT CCACCCACAG 
GATTTCTCTG ATGTCATTAG TTTTATTTTG TATGGAAGCA CTCATCGAAC TGGGAAAGAT 
AATTGGTTGG AAAGGTTATT CTATAGCTGT CAAAGGCTGG ATAAACGAGA TCAACCAACC 
ATCCCTCGTA ATCTGTTGAA GACTGATGCT GTTCTCTGGC AATGGGCTGT TTGGGAAGCA 
GCTCAGTTTA CTGTTCTTTC TAAGCTGAGA ACTCCTCTGG GTAGAGCCCA AGATACATTT 
CAAACAATTG AAGGTATTAT TAGGAGTCTT GCAGCGCATA CGCTAAACCC TGATCAAGAT 
GTTAGCCAGT GGACTACTGC AGACAATGAT GAGGGACACA GCAGCAATCA ACTTAGGCTT 
GTTCTCCTTC TACAGTATCT GGAGAACTTG GAAAAACTGA TGTACAATGC TTATGAAGGA 
TGTGCCAATG CCCTTACCTC TCCACCCAAG GTTATCAGGA CATTCTTTTA TACTAATCGC 
CAGACGTGCC AAGATTGGCT GACGCGGATT AGACTTTCCA TCATGAGAGT TGGACTGCTG 
GCCGGCCAGC CTGCTGTGAC AGTCAGACAT GGTTTTGACT TACTCACAGA AATGAAAACT 
AATAACAGTT TATCTCAGGG AAATGAATTG GAAGTGACAA TTATGATGCT GGTGGAAGCA 
TTATGTGAAC TTCACTGCCC TGAAGCTATT CAGGGTATTG CTGTCTGGTC TTCTGCTGTA 
GTCGGGAAGA GTCTTACCTG GATCAACTCT GTAGCTCAAC AGGCAGAAGG GCGGTTTGAA 
AAAGCAACTG CGGAGTACCA GGAGCACCTG TGTGCCATGA CAGGAGTAGA TTGCTGTATA 
TCGGGATTTG ACAAAACTGT TCTGAAGTTG GCCAATTCCA ACAGTGTGAA TAACGCCAGC 
CCAAAGCACT CCTTGAACGG AGAAACTAGA AAAACTGTTC TGACTAAGCC AAGTGACTGT 
TCACCTGAAG TTATAAATTA CTTGGGTAAC AAGGCATGTG AATGTTACAT TTCAATTGCT 
GATTGGTCTG CTGTTCAGGA ATGGCAAAAT ATGGTCCATG ACTTGAAGAA AAGCACGAGT 
AATACCTCAA TCAATCTGAA AGCAGATTTT AACTACATCA AATCCCTGAG CAGCTTTGAG 
TCTGGTGAAC TTACTGAATG CACTGAACAA CTGGAATTAT TACCAGGAGA AAATATCAAC 
CTACTTGCAG GGGGATCCAA AGAAAAAATA GACATGAAGA AGCTCCTTCC TAATATGCTA 
AGTCCTGACC CGAGAGAACT TCAAAAAGCA GTTGAAGTAC AACTTCTGAG AAGTTCGGTG 
TGTCTGGCAA CAGCTGTAAA CCACATGGAG CAGGAGCAAA AGTGGCAGTC AATATCTGAA 
AATGTTATAA AATACTTAAA GCAAACATCA CGAATAGCTA TTGGGCCTTT AAGACTTTCC 
ACGCTCACCG TGTCTCAGTC TTTACCAGTG TTGAGCACTC TTCAGTTGTA TTGTTCTTCT 
GCTTTAGAAA ACACCGTATC CAACAGGCTG ACATCAGAGG ACTGTCTTAT ACCTCTCTTC 
AATGATGCTC TGAGGTCATG TAAACAGCAT GATGTGAGGC CGTGGATGCA TGCACTGAGA 
TACACGGTGT ATCAGAATCA ACTGATGGGA AAGCTTAAAG AACCAACAGT CCCAATTAAG 
AGTCATCTAA TGGAGCTGGG CTTAACAGCA GCAAAATTTG CACGGAAGCG AGGAAATATT 
GCCCTGGCTA CACGGTTGCT TGCCCAGTGC AGTGAAGTTC AGCTAGGAAA AACCACCACT 
GCACAGGACT TAGTCCAGCA CTTTAAGAAA CTGTCTGCAC CAGGTCAGAT TGATGAAAAG 
TGGGGTCCTG AACTTGATAT TGAGAAAACA AAGCTGTTAT ACACAGCAGG TCAGTCAACA 
CATGCCATGG AAATGCTGAG TTCTTGTGCT ATATCATTTT GCAAATCTGC CAAGGCTGAA 
TATGCAGTTG CTAAATCTAT TCTTACGCTG GCTAAATGGA TTCAAGCAGA ATGGAAAGAG 
ATTTCAGGAC AGTTAAAACA AGTATATAGA GCTCGTCAAC AGCAGAATCA CTCTGGTCTG 
TCTACCTTGT CTAAGAACAT ATATACTTTG ATTGATCTCC CAGCTGTTAA TACGTTAGAA 
GAGGAATATC CCAGGATTGA AAGTGAATCT ACAGTAAATA TTGGAGTTGG AGAACCTGAC 
TTTATCTTGG GACAGCTGTA TCACTTGTCT TCAGTACAGG CACCTGAAGT GGCCAAGTCT 
TGGGCAGCCC TGGCTAGTTG GGCTTATAGA TGGGGCCGGA AAGTAGTTGA TAATGCCAGT 
CAGGGAGAAG GTGTTCGTCT GCTGCCCCGA GAGAAATCTG AGGTTCAGAG CTTGCTTCCA 
GACAATGTTA CAGAGGAAGA CAAAGAGAAG ATTTATGGGA TCCTTGGGCA AGCAGTGTGT 
CGTCCAGCTG GGATTCAAGA TGAAGATATG ACTCTGCAAA TCACTGAAAA TGAAGACAAT 
GAAGAAGATG ATATGGTGGA TGTTATATGG CGTCAGTTAT TAACAACTTG TCCCTGGCTT 
TCAGATCTTG ATGAAAATGC AACAGAAGGT TTAATTAAAG TATGGCGGAA AGTTGTAGAC 
AGAATCTTCA GTCTCTATAA ACTCTCCTGC AGTGCATATT TCACTTTCCT CAAACTCAAT 
GCTGGTCAGG TTCCACTTGA TGAGGATGAT CCAAGGCTGC ATTTAAGAAA TACTTCTGAG 
CAAAGCACTG ATGATGTTAT TGTGATGGCA ACCCTAAGAC TGTTAAGGCT GCTTGTTAAA 
CATGCTGGAG AGCTTAGACA GTATCTAGAG CATGGGCTAG AAACTACACC TACTGCTCCT 
TGGAGAGGTA TTATTCCTCA GCTCTTCTCT CGCCTCAATC ATCCAGAAGT GTATGTTCGT 
CAGAGTATTT GCAACTTACT GTGTCGAGTG GCTCAAGATT CTCCTCATCT CATACTGTAT 
CCTGCAATAG TGGGTACTAT TTCACTCAGC AGTGAATCCC AAACCTCAGG GAACAAGCTT 
CCTTCAGCGA TTCCCACTTT GCTAGGTAAT ATTCAAGGAG AAGAACTGTT GGGTGGTGAA 
TGTGATGGTG GGAGTCCACC AGCTTCTCAA GAAAGCACTA AAGATGACAC AAAAGTTGGA 
TTAAATGAAG ATCAAGCAAT GATGCAAGAT TGTTACAGCA AAATTGTGGA GAAACTCTCA 
ACTGCAAATC CAACAATGGT ATTGCAGGTT CAGATGCTAG TGGCTGAGCT GCGCAGAGTT 
ACAGTTCTGT GGGATGAACT TTGGTTGGGC GTTTTACTGC AGCAGCACAT GTATGTCCTC 
AGAAGGATTC AGCAACTGGA AGATGAAGTC AAAAGGGTCC AAAACAACAA CACTCTACGC 
AAAGAGGAGA AGATAGCAAT CATGCGTGAG AAGCATACAG CTCTAATGAA GCCCATTGTA 
TTTGCTTTGG AACATGTACG GAGCATCACT GCAGCTCCTA CAGAAACTCC TCATGAGAAA 
TGGTTTCAGG ATAACTATGG CGGTGCAATC GAAAATGCAC TAGAGAAACT TAAGAATCCT 
TTGAATCCTG CTAAGCCTGG AAGCAGCTGG CTCCCCTTTA AAGAGGTTAT GCTAAGTTTG 
CAACAGAGAG CACAGAAACG GGCTAGTTAT CTTTTACGGC TTGAAGAAAT CAGTCCTTGG 
TTGGCTGCCA TGATGAACAC TGAAATAGCG CTCCCTGGAG AAGTATCCAC CAGAGACACT 
GTCACAATTC ATAGTGTGGG CAGCACCATC ACAATTCTGC CTACCAAAAC CAAACCTAAA 
AAGCTGCTTT TCCTGGGCTC AGATGGGAAG AACTATCCAT ACTTGTTCAA AGGTTTAGAG 
GACTTGCACC TAGATGAAAG AATCATGCAA TTCCTATCAA TTGTGAACAC AATGTTTGCT 
ACCATTAACC GTCAAGAGAC ACCAAGGTTT CATGCACGGC ACTACTCTGT GACACCTCTG 
GGAACAAGAT CAGGCCTGAT CCAGTGGGTG GATGGAGCTA CACCTTTGTT TGGTCTTTAC 
AAACGATGGC AACAGCGGGA AGCTGCTTTA CAAGCACAAA AGGCTCAGGA TTCTTATCAG 
ACTCCTCAGA ATCCTGGAAT CGTGCCTAGA CCTAGTGAAC TGTACTACAG TAAAATTGGA 
CCTGCTTTAA AGGCAGTTGG GCTTAGTCTT GATGTGTCGC GTCGTGACTG GCCCTTGAAT 
GTGATGAGAG CTGTTCTAGA AGAACTGATG GAAGCAACTC CCCCAAATCT CCTAGCTAAG 
GAGCTCTGGT CGTCATGTAC TACACCAGAT GAATGGTGGA GAGTTACACA GTCTTATGCA 
AGATCCACTG CAGTTATGTC CATGGTTGGC TACATCATTG GTCTTGGAGA CAGACATCTG 
GATAATGTTC TTATAGATAT GACTACAGGA GAAGTTGTCC ACATAGATTA TAATGTTTGC 
TTTGAAAAAG GGAAGAGCCT TAGGGTACCA GAGAAGGTTC CATTCCGAAT GACACACAAT 
ATTGAAACAG CACTTGGAGT GACAGGAGTA GAGGGGGTTT TCAGGCTTTC TTGTGAACAG 
GTCCTTCATA TCATGCGGCG TGGGAGAGAA ACTTTGCTGA CGTTGCTTGA AGCTTTTGTT 
TATGATCCTC TGGTGGACTG GACAGCAGGG GGTGAGGCAG GATTTGCAGG AGCTGTGTAT 
GGTGGTGGCG GCCAGCAGGC TGAAAACAAG CAAAGCAAAA GAGAAATGGA AAGAGATATT 
ACACGTAGTC TCTTCTCTTC AAGAGTGGCT GAGATAAAGG TTAACTGGTT TAAGAACAGA 
GATGAAATGT TGGCTGTGCT TCCAAAGTTG GACAGTAGTT TAGAAGAATA TCTCAACCTG 
CAAGAACAGT TGACAGATGT AGAAAAATTG CAAGGAAAGC TACTGGAGGA GATAGAATTT 
CTTGAAGGTG CAGAAGGAGT GGATCATCCC TCTCACACAC TTCAGCACAG GTATTCTGAG 
CACACACAGT TGCAGACTCA ACAAAGAGCT GTCCAGGAGG CGATCCAGGT GAAGCTGAAT 
GAATTTGAGC AGTGGATAAC ACACTACCAA GCTGCTTTCA GTAATTTAGA GGCAACACAA 
CTTGCAAGTC TCCTTCAAGA AATTAGCACA CAAATGGACC TAGGTCCTCC GAGTTATGTC 
CCAGCAACAG CATTTCTGCA AAATGCAGGC CAGGCACATC TCATCAGTCA GTGTGAACAA 
TTAGAAGGGG AGGTTGGTGC TCTTCTCCAG CAGAGAAGAT CTGTTTTACG TGGTTGCCTC 
GAACAGCTGC ACAACTATGC AACAGTGGCT CTTCAGTATC CGAAAGCTGT GTTTCAGAAG 
CACCGAATAG AGCAGTGGAA AACTTGGATG GAAGAACTCA TCTGCAACAT GACAATAGAG 
CGCTGTCAGG ATATCTTTAG GAAGTATGAA ATGCAGTATG CTCCGCAGCC ACCTCCAAGT 
GTGTGCCAGT TCATAACCGC GACGGAAATG ACTCTCCAGC GCTATGCAGC AGATATAAAT 
AGCAGACTTA TCAGGCAGGT GGAGCGTCTG AAGCAGGAAG CAGTTACTGT ACCTGTTTGT 
GAAGAGCAGC TGAAAGAAAT CGAACGCTGC ATAAAAGTTT TCCTTCATGA AAATGGAGAG 
GAGGGCTCAC TGAGCTTAGC AAGTGTCATC ATCTCTGCCC TTTGTACGCT GACGAGGCGG 
AATCTGATGA TGGAAGGTGC AGCATCCAGT GCTGGAGAGC AGCTGGTTGA TCTGACTTCG 
AGAGATGGAG CATGGTTTTT GGAGGAGCTC TGCAGCATGA GTGGGAATGT TACTTGCCTT 
GTGCAGTTGC TGAAGCAGTG TCATCTTGTA CCCCAGGACT TAGATATTCC TAACCCAATT 
GAAGCATCAG AAGCTGTTCA TTTAGCTAAT GGAGTATACA TCTCACTACA GGAACTGAAT 
TCCAACTTCC GGCAGATTAT TTTCCCTGAA GCACTTCGGT GCTTAATGAA AGGAGAATAC 
ACTTTAGAAA GTATGCTCCA TGAATTAGAT AACCTTATTG AACAAACAAC TGATGGAGTT 
TCTTTGCAAA CATTGGTTGA ATCTCTTCAG GCCTACTTAA GAAATGCAGC TATGGGACTA 
GAGGAAGAAA CACACACTCA TTACATTGAT GTTGCAAGAG TACTTCATGC TCAGTATGGC 
GAGCTGATTC AGCCAAGGAA TGGTTCTGTG GATGAAACTC CCAAAATGTC AGCTGGTCAG 
ATGCTTTTGG TAGCCTTTGA TGGAATGTTT GCTCAGGTGG AAACTGCGTT TGGTTTATTG 
ATTGAAAAGC TAAACAAGAT GGAAGTCCCT GTTGCTTGGC GTAAGATCGA CATCATTAGG 
GAAGCCCGCA GCACTCAAGT TAACTTCTTT GATGATGACA ATAATCGCCA AGTTCTGGAG 
GAAATCTTCT TTTTGAAGAG ACTGCAAACA ATTAAAGAAT TTTTCAGACT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG AATAAGTTCA CTTGATGACC AGAATGCAGT AAACGGACCT 
GTTCAGATTG TCAATGTGAA AGCCCTTTAC AGAAACTCTT GCTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCTTT TACCGCTGAC TTTGTGAGGC AGCTTTTAAT TGGCCTTCCA 
AATCAGGCTT TAGGACTGAC TTTGTGCAGT TTCATCAGCG CTTTGGGCGT GGATATCATA 
GCTCAGGTAG AAGCCAAAGA CTTTGGAGCA GAAAGCAAAG TTTCTGTGGA TGACCTGTGC 
AAGAAAGCTG TGGAGCACAA TATCCAGATT GGCAAGTTCT CACAGCTGGT CATGAACAGG 
GCAACAGTGC TAGCTAGCTC GTATGATACA GCGTGGAAGA AGCATGATCT GGTGCGCAGA 
CTCGAAACCA GTATTTCTTC TTGCAAGACC AGTCTTCAGA GAGTGCAGCT GCATATTGCC 
ATGTTCCAAT GGCAGCATGA AGATCTCCTT ATCAGTCGCC CACAAGCCAT TTCTGTTACT 
CCTCCACCTC GTTCAGCAAT TTTAGCCAAC ATGAAGAAGA AACTTCATAC GCTCAGCCAG 
ATTGAAGCTT CAATTGCAAC AGTTCAGGAG AAACTAGCAG CACTTGAAGC AAGCATTGAA 
CAGCGTTTGA AATGGGCAGG AGGAGCTAAT CCTGCACTGG CACCAGTCTT GCAAGATTTC 
GATGCCACTA TTGCTGAAAG AAGAAGCCTT GTCCTGAAAG AAAGTCAAAG AGCAAGTCAG 
GTTACTTTCC TGTGCAGTAA TATCATCCAC TTTGAAAGTT TACGTACTAG AACAGCAGAA 
GCCTTAAATC TTGATGCTGC ATTATTTGAA CTTCTCAAAC GCTGTCAACA AATGTGTTCA 
TTTGCATCAC AGTTCAACAG CTCAGTGTCT GAATTGGAGC TTCGTCTGCT GCAGAGAGTG 
GGCACTGGTC TTGAACATCC TATTGGCAGC TCTGAGTGGC TTCACTCAGC CCACAAGCAG 
TTGACACAGG AAATTACTAC ACAGAGAACT GTACAGGCAG AAAGAGAACA GCAAATAGAA 
ACAGTTTGTG AAACTATTCA AAATCTGGTG GATAATATTA AAACTGTGCT CACAGGTCAT 
AATAGACAAC TTGGAGATGT CAAGCATCTA CTGAAGGCTA TGGCAAAGGA TGAAGAAGCA 
GCTTTGGCAG ATGGTGAAGA TGTTCCCTAT GAAAATAGTG TTAGGCAGTT CTTGGGCGAA 
TATAAATCAT GGCAAGATAA TATTCAGACA GTTTTGTTTA CATTAGTTCA GGTTATGGGT 
CAAGTCCGCA ATCAGGAACA TGTTGAAATG CTGCAGGAAA TAACCCCCAC CCTGAAAGAG 
CTAAAAACAC AGAGCCAAAG TGTCTACAAT AATCTAGTGA GTTTTGCATC ACCCTTAGTC 
ACGGATACAT CAAATGAATG CTCAAGCCCA ACATCAGCTG CTACATACCA GCCAACCTTT 
GCTGCAGCTG TACGTAGCAA TACAGGGCAG AAAACTCAGC CAGAGGTGAT GTCACAGAAT 
GCAAGAAAGC TAATTCAGAA AAATCTTGCC ACATCAGCAG ATACTCCTCC AAGCACAGTC 
CCAGGAACTG GCAAAAGTGT TGCCTGTAGT CCCAAGAAGA CAGCCAGGGA CCCTAAAACA 
GGAAAAGCTG TGCAGGAAAG GAATTCATAT GCAGTTAGTG TGTGGAAACG AGTCAAAGCC 
AAGTTGGAGG GCCGAGATGT GGATCCCAGC AGGAGAATGT CTGTTGCTGA ACAGGTTGAC 
TTTGTGATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACT 
GCCTGGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150021.1
CDS	249..11000
Translation	MTGNPGGYSVNGGSGENTYGRKSLGQELRVNNVTNPDFTSVPHGNRALATKDMRKSQERSLSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILTAIHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSLSTKDEVKLLYLCATYKALETVGEKKAFSSLMQLVMTSLQSILENVDTPELLCKCVKCILLLSRCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLTFSTTLLGQFLEDMEAYAEDLSHVASGESLDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQMENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPESKLLVLRYHKEKEVVAAAHAVYQAVLSLKNIPVLETAYRLILGEMTCALNSLLYSLHLPEACSEIQHDSFKKHVFTVENAKFVVIFDLSALSTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDIAVHFPAVQYAVLYTLYSHCSRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGLLLKKDNLTQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLLEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGARVRQTFGKLLKSIPLDVVLSNRNHTEIQEISLAIRSHMSKAPSNTFHPQDFSDVISFILYGSTHRTGKDNWLERLFYSCQRLDKRDQPTIPRNLLKTDAVLWQWAVWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNNSLSQGNELEVTIMMLVEALCELHCPEAIQGIAVWSSAVVGKSLTWINSVAQQAEGRFEKATAEYQEHLCAMTGVDCCISGFDKTVLKLANSNSVNNASPKHSLNGETRKTVLTKPSDCSPEVINYLGNKACECYISIADWSAVQEWQNMVHDLKKSTSNTSINLKADFNYIKSLSSFESGELTECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKAVEVQLLRSSVCLATAVNHMEQEQKWQSISENVIKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLTSEDCLIPLFNDALRSCKQHDVRPWMHALRYTVYQNQLMGKLKEPTVPIKSHLMELGLTAAKFARKRGNIALATRLLAQCSEVQLGKTTTAQDLVQHFKKLSAPGQIDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRARQQQNHSGLSTLSKNIYTLIDLPAVNTLEEEYPRIESESTVNIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQSLLPDNVTEEDKEKIYGILGQAVCRPAGIQDEDMTLQITENEDNEEDDMVDVIWRQLLTTCPWLSDLDENATEGLIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVPLDEDDPRLHLRNTSEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQTSGNKLPSAIPTLLGNIQGEELLGGECDGGSPPASQESTKDDTKVGLNEDQAMMQDCYSKIVEKLSTANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPTETPHEKWFQDNYGGAIENALEKLKNPLNPAKPGSSWLPFKEVMLSLQQRAQKRASYLLRLEEISPWLAAMMNTEIALPGEVSTRDTVTIHSVGSTITILPTKTKPKKLLFLGSDGKNYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLNVMRAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLAVLPKLDSSLEEYLNLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFSNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHNYATVALQYPKAVFQKHRIEQWKTWMEELICNMTIERCQDIFRKYEMQYAPQPPPSVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEEQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPIEASEAVHLANGVYISLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDNLIEQTTDGVSLQTLVESLQAYLRNAAMGLEEETHTHYIDVARVLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLIEKLNKMEVPVAWRKIDIIREARSTQVNFFDDDNNRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGISSLDDQNAVNGPVQIVNVKALYRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLISRPQAISVTPPPRSAILANMKKKLHTLSQIEASIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFDATIAERRSLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELLKRCQQMCSFASQFNSSVSELELRLLQRVGTGLEHPIGSSEWLHSAHKQLTQEITTQRTVQAEREQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQVMGQVRNQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDTSNECSSPTSAATYQPTFAAAVRSNTGQKTQPEVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKTARDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPSRRMSVAEQVDFVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_015294535.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294535.2

ORF Insert Sequence:

ATGACTGGCA ACCCAGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGAGTT AACAATGTGA CCAATCCTGA TTTTACCAGT 
GTTCCGCATG GGAATCGTGC TTTAGCCACA AAAGACATGA GGAAATCACA GGAGCGATCG 
TTGTCTTATT CTGACGAGTC TCGACTGTCA AATCTTCTTC GGAGGATTAC TCGGGAAGAC 
GACAGAGACC GAAGACTGGC TACTGTAAAG CAGCTGAAAG AATTCATTCA GCAACCAGAA 
AACAAACTGG TACTAGTTAA ACAACTGGAT AATATCCTGA CTGCCATTCA TGATGTACTC 
AATGAAAGTA GCAAATTGCT TCAAGAACTG AGACAGGAAG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTAAGCTA TGAGGCTGAG AAGATATTTA AATGGATTTT TAGCAAATTC 
AGCTTATCCA CAAAAGATGA AGTTAAACTT CTTTATTTGT GTGCAACCTA CAAAGCACTG 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTTTAATGC AGCTTGTAAT GACCAGCCTA 
CAGTCCATTC TAGAAAATGT GGATACACCA GAGTTACTGT GCAAATGTGT CAAGTGCATT 
CTTCTGTTGT CCCGATGTTA TCCGCACATT TTCAGCACCA ATTTTCGGGA CACAGTGGAC 
ATACTGGTTG GGTGGCATAT AGATCACACT CAGAAGCCTT CATTGACTCA GCAGGTGTCC 
GGGTGGCTGC AGAGCTTGGA GCCCTTTTGG GTGGCTGATC TCACTTTTTC TACCACACTC 
TTGGGTCAAT TTTTAGAAGA TATGGAAGCT TATGCTGAGG ACCTCAGCCA TGTGGCTTCT 
GGGGAGTCCC TAGATGAAGA TATTCCTCCT CCCTCTGTAT CACTACCTAA ACTGGCTGCG 
CTTCTTCGAG TTTTCAGTAC TGTGGTGAGA AGTATTGGGG AGCGCTTCAG CCCAATTAGA 
GGACCACCGA TTACAGAAGC ATATGTAACT GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACTGCAGCAA ACCAAGTATT CTTTTCAGAA GCAGTACTCA CAGCTGCCAA TGAGTGTGTT 
GGTGTTTTAC TTGGCAGTCT GGATCCAAGC ATGACCATTC ACTGTGACAT GGTTATCACC 
TACGGATTGG ATCAAATGGA AAATTGTCAG ACTTGTGGCA CTGATTATAT CATTTCAGTC 
CTGAATCTGT TGACGCTGAT TGTTGAACAA ATAAATACAA AACTACCATC GTCATTTGTA 
GAGAAATTAT TTATTCCAGA GTCTAAACTG CTTGTACTTC GTTATCATAA GGAAAAAGAG 
GTTGTTGCAG CAGCCCACGC TGTGTATCAG GCAGTACTGA GCCTGAAGAA CATTCCTGTT 
TTGGAGACTG CTTACAGGTT GATTCTAGGA GAAATGACCT GTGCTCTAAA CAGTCTTCTT 
TATAGTTTAC ATCTTCCCGA GGCCTGTTCT GAAATCCAGC ATGACTCTTT CAAGAAGCAT 
GTATTCACTG TGGAAAATGC AAAGTTTGTT GTTATATTTG ACCTCAGTGC CCTAAGTACT 
ATTGGAAATG CTAAGAACTC ATTAATTGGG ATGTGGGCCC TTTCGCCAAC TGTGTTTGCA 
CTACTGAGTA AAAATCTGAT GATTGTTCAT GGTGACATAG CAGTTCATTT TCCTGCTGTC 
CAGTATGCAG TACTCTATAC GTTGTATTCG CATTGTTCCA GACATGACCA TTTTATATCC 
AGTAGCCTCA GTTCTTCCTC TCCTTCCCTG TTCGATGGTG CAGTTATTAG TACTGTAACC 
ACAGCAACAA AAAAACATTT CTCAATCATA CTGAACCTTT TGGGGCTACT GTTGAAGAAG 
GATAACCTTA CTCAAGATAC CAGGAAACTG CTTATGACGT GGGCTTTGGA AGTGGCTGTT 
CTGATGAAGA AATCAGAGAC ATATGCACCC TTATTTTCTC TCCCATCTTT CCATAAGTTT 
TGCAAAGGAC TTCTAGCAAA CACCCTCCTG GAAGATGTGA ATATTTGTCT TCAAGCGTGT 
AGCAGCCTCC ATGCCCTGTC CTCCTCCCTA CCAGATGACC TTTTACAGAG ATGTGTTGAT 
GTGTGTCGTG TTCAACTAGT TCATAGCGGT GCTCGTGTAA GGCAGACTTT CGGAAAACTT 
CTGAAGTCAA TTCCCTTAGA TGTGGTGTTG AGCAACCGTA ACCACACAGA AATACAAGAA 
ATTTCTTTGG CTATAAGAAG TCATATGAGC AAAGCTCCAA GTAATACATT CCACCCACAG 
GATTTCTCTG ATGTCATTAG TTTTATTTTG TATGGAAGCA CTCATCGAAC TGGGAAAGAT 
AATTGGTTGG AAAGGTTATT CTATAGCTGT CAAAGGCTGG ATAAACGAGA TCAACCAACC 
ATCCCTCGTA ATCTGTTGAA GACTGATGCT GTTCTCTGGC AATGGGCTGT TTGGGAAGCA 
GCTCAGTTTA CTGTTCTTTC TAAGCTGAGA ACTCCTCTGG GTAGAGCCCA AGATACATTT 
CAAACAATTG AAGGTATTAT TAGGAGTCTT GCAGCGCATA CGCTAAACCC TGATCAAGAT 
GTTAGCCAGT GGACTACTGC AGACAATGAT GAGGGACACA GCAGCAATCA ACTTAGGCTT 
GTTCTCCTTC TACAGTATCT GGAGAACTTG GAAAAACTGA TGTACAATGC TTATGAAGGA 
TGTGCCAATG CCCTTACCTC TCCACCCAAG GTTATCAGGA CATTCTTTTA TACTAATCGC 
CAGACGTGCC AAGATTGGCT GACGCGGATT AGACTTTCCA TCATGAGAGT TGGACTGCTG 
GCCGGCCAGC CTGCTGTGAC AGTCAGACAT GGTTTTGACT TACTCACAGA AATGAAAACT 
AATAACAGTT TATCTCAGGG AAATGAATTG GAAGTGACAA TTATGATGCT GGTGGAAGCA 
TTATGTGAAC TTCACTGCCC TGAAGCTATT CAGGGTATTG CTGTCTGGTC TTCTGCTGTA 
GTCGGGAAGA GTCTTACCTG GATCAACTCT GTAGCTCAAC AGGCAGAAGG GCGGTTTGAA 
AAAGCAACTG CGGAGTACCA GGAGCACCTG TGTGCCATGA CAGGAGTAGA TTGCTGTATA 
TCGGGATTTG ACAAAACTGT TCTGAAGTTG GCCAATTCCA ACAGTGTGAA TAACGCCAGC 
CCAAAGCACT CCTTGAACGG AGAAACTAGA AAAACTGTTC TGACTAAGCC AAGTGACTGT 
TCACCTGAAG TTATAAATTA CTTGGGTAAC AAGGCATGTG AATGTTACAT TTCAATTGCT 
GATTGGTCTG CTGTTCAGGA ATGGCAAAAT ATGGTCCATG ACTTGAAGAA AAGCACGAGT 
AATACCTCAA TCAATCTGAA AGCAGATTTT AACTACATCA AATCCCTGAG CAGCTTTGAG 
TCTGGTGAAC TTACTGAATG CACTGAACAA CTGGAATTAT TACCAGGAGA AAATATCAAC 
CTACTTGCAG GGGGATCCAA AGAAAAAATA GACATGAAGA AGCTCCTTCC TAATATGCTA 
AGTCCTGACC CGAGAGAACT TCAAAAAGCA GTTGAAGTAC AACTTCTGAG AAGTTCGGTG 
TGTCTGGCAA CAGCTGTAAA CCACATGGAG CAGGAGCAAA AGTGGCAGTC AATATCTGAA 
AATGTTATAA AATACTTAAA GCAAACATCA CGAATAGCTA TTGGGCCTTT AAGACTTTCC 
ACGCTCACCG TGTCTCAGTC TTTACCAGTG TTGAGCACTC TTCAGTTGTA TTGTTCTTCT 
GCTTTAGAAA ACACCGTATC CAACAGGCTG ACATCAGAGG ACTGTCTTAT ACCTCTCTTC 
AATGATGCTC TGAGGTCATG TAAACAGCAT GATGTGAGGC CGTGGATGCA TGCACTGAGA 
TACACGGTGT ATCAGAATCA ACTGATGGGA AAGCTTAAAG AACCAACAGT CCCAATTAAG 
AGTCATCTAA TGGAGCTGGG CTTAACAGCA GCAAAATTTG CACGGAAGCG AGGAAATATT 
GCCCTGGCTA CACGGTTGCT TGCCCAGTGC AGTGAAGTTC AGCTAGGAAA AACCACCACT 
GCACAGGACT TAGTCCAGCA CTTTAAGAAA CTGTCTGCAC CAGGTCAGAT TGATGAAAAG 
TGGGGTCCTG AACTTGATAT TGAGAAAACA AAGCTGTTAT ACACAGCAGG TCAGTCAACA 
CATGCCATGG AAATGCTGAG TTCTTGTGCT ATATCATTTT GCAAATCTGC CAAGGCTGAA 
TATGCAGTTG CTAAATCTAT TCTTACGCTG GCTAAATGGA TTCAAGCAGA ATGGAAAGAG 
ATTTCAGGAC AGTTAAAACA AGTATATAGA GCTCGTCAAC AGCAGAATCA CTCTGGTCTG 
TCTACCTTGT CTAAGAACAT ATATACTTTG ATTGATCTCC CAGCTGTTAA TACGTTAGAA 
GAGGAATATC CCAGGATTGA AAGTGAATCT ACAGTAAATA TTGGAGTTGG AGAACCTGAC 
TTTATCTTGG GACAGCTGTA TCACTTGTCT TCAGTACAGG CACCTGAAGT GGCCAAGTCT 
TGGGCAGCCC TGGCTAGTTG GGCTTATAGA TGGGGCCGGA AAGTAGTTGA TAATGCCAGT 
CAGGGAGAAG GTGTTCGTCT GCTGCCCCGA GAGAAATCTG AGGTTCAGAG CTTGCTTCCA 
GACAATGTTA CAGAGGAAGA CAAAGAGAAG ATTTATGGGA TCCTTGGGCA AGCAGTGTGT 
CGTCCAGCTG GGATTCAAGA TGAAGATATG ACTCTGCAAA TCACTGAAAA TGAAGACAAT 
GAAGAAGATG ATATGGTGGA TGTTATATGG CGTCAGTTAT TAACAACTTG TCCCTGGCTT 
TCAGATCTTG ATGAAAATGC AACAGAAGGT TTAATTAAAG TATGGCGGAA AGTTGTAGAC 
AGAATCTTCA GTCTCTATAA ACTCTCCTGC AGTGCATATT TCACTTTCCT CAAACTCAAT 
GCTGGTCAGG TTCCACTTGA TGAGGATGAT CCAAGGCTGC ATTTAAGAAA TACTTCTGAG 
CAAAGCACTG ATGATGTTAT TGTGATGGCA ACCCTAAGAC TGTTAAGGCT GCTTGTTAAA 
CATGCTGGAG AGCTTAGACA GTATCTAGAG CATGGGCTAG AAACTACACC TACTGCTCCT 
TGGAGAGGTA TTATTCCTCA GCTCTTCTCT CGCCTCAATC ATCCAGAAGT GTATGTTCGT 
CAGAGTATTT GCAACTTACT GTGTCGAGTG GCTCAAGATT CTCCTCATCT CATACTGTAT 
CCTGCAATAG TGGGTACTAT TTCACTCAGC AGTGAATCCC AAACCTCAGG GAACAAGCTT 
CCTTCAGCGA TTCCCACTTT GCTAGGTAAT ATTCAAGGAG AAGAACTGTT GGGTGGTGAA 
TGTGATGGTG GGAGTCCACC AGCTTCTCAA GAAAGCACTA AAGATGACAC AAAAGTTGGA 
TTAAATGAAG ATCAAGCAAT GATGCAAGAT TGTTACAGCA AAATTGTGGA GAAACTCTCA 
ACTGCAAATC CAACAATGGT ATTGCAGGTT CAGATGCTAG TGGCTGAGCT GCGCAGAGTT 
ACAGTTCTGT GGGATGAACT TTGGTTGGGC GTTTTACTGC AGCAGCACAT GTATGTCCTC 
AGAAGGATTC AGCAACTGGA AGATGAAGTC AAAAGGGTCC AAAACAACAA CACTCTACGC 
AAAGAGGAGA AGATAGCAAT CATGCGTGAG AAGCATACAG CTCTAATGAA GCCCATTGTA 
TTTGCTTTGG AACATGTACG GAGCATCACT GCAGCTCCTA CAGAAACTCC TCATGAGAAA 
TGGTTTCAGG ATAACTATGG CGGTGCAATC GAAAATGCAC TAGAGAAACT TAAGAATCCT 
TTGAATCCTG CTAAGCCTGG AAGCAGCTGG CTCCCCTTTA AAGAGGTTAT GCTAAGTTTG 
CAACAGAGAG CACAGAAACG GGCTAGTTAT CTTTTACGGC TTGAAGAAAT CAGTCCTTGG 
TTGGCTGCCA TGATGAACAC TGAAATAGCG CTCCCTGGAG AAGTATCCAC CAGAGACACT 
GTCACAATTC ATAGTGTGGG CAGCACCATC ACAATTCTGC CTACCAAAAC CAAACCTAAA 
AAGCTGCTTT TCCTGGGCTC AGATGGGAAG AACTATCCAT ACTTGTTCAA AGGTTTAGAG 
GACTTGCACC TAGATGAAAG AATCATGCAA TTCCTATCAA TTGTGAACAC AATGTTTGCT 
ACCATTAACC GTCAAGAGAC ACCAAGGTTT CATGCACGGC ACTACTCTGT GACACCTCTG 
GGAACAAGAT CAGGCCTGAT CCAGTGGGTG GATGGAGCTA CACCTTTGTT TGGTCTTTAC 
AAACGATGGC AACAGCGGGA AGCTGCTTTA CAAGCACAAA AGGCTCAGGA TTCTTATCAG 
ACTCCTCAGA ATCCTGGAAT CGTGCCTAGA CCTAGTGAAC TGTACTACAG TAAAATTGGA 
CCTGCTTTAA AGGCAGTTGG GCTTAGTCTT GATGTGTCGC GTCGTGACTG GCCCTTGAAT 
GTGATGAGAG CTGTTCTAGA AGAACTGATG GAAGCAACTC CCCCAAATCT CCTAGCTAAG 
GAGCTCTGGT CGTCATGTAC TACACCAGAT GAATGGTGGA GAGTTACACA GTCTTATGCA 
AGATCCACTG CAGTTATGTC CATGGTTGGC TACATCATTG GTCTTGGAGA CAGACATCTG 
GATAATGTTC TTATAGATAT GACTACAGGA GAAGTTGTCC ACATAGATTA TAATGTTTGC 
TTTGAAAAAG GGAAGAGCCT TAGGGTACCA GAGAAGGTTC CATTCCGAAT GACACACAAT 
ATTGAAACAG CACTTGGAGT GACAGGAGTA GAGGGGGTTT TCAGGCTTTC TTGTGAACAG 
GTCCTTCATA TCATGCGGCG TGGGAGAGAA ACTTTGCTGA CGTTGCTTGA AGCTTTTGTT 
TATGATCCTC TGGTGGACTG GACAGCAGGG GGTGAGGCAG GATTTGCAGG AGCTGTGTAT 
GGTGGTGGCG GCCAGCAGGC TGAAAACAAG CAAAGCAAAA GAGAAATGGA AAGAGATATT 
ACACGTAGTC TCTTCTCTTC AAGAGTGGCT GAGATAAAGG TTAACTGGTT TAAGAACAGA 
GATGAAATGT TGGCTGTGCT TCCAAAGTTG GACAGTAGTT TAGAAGAATA TCTCAACCTG 
CAAGAACAGT TGACAGATGT AGAAAAATTG CAAGGAAAGC TACTGGAGGA GATAGAATTT 
CTTGAAGGTG CAGAAGGAGT GGATCATCCC TCTCACACAC TTCAGCACAG GTATTCTGAG 
CACACACAGT TGCAGACTCA ACAAAGAGCT GTCCAGGAGG CGATCCAGGT GAAGCTGAAT 
GAATTTGAGC AGTGGATAAC ACACTACCAA GCTGCTTTCA GTAATTTAGA GGCAACACAA 
CTTGCAAGTC TCCTTCAAGA AATTAGCACA CAAATGGACC TAGGTCCTCC GAGTTATGTC 
CCAGCAACAG CATTTCTGCA AAATGCAGGC CAGGCACATC TCATCAGTCA GTGTGAACAA 
TTAGAAGGGG AGGTTGGTGC TCTTCTCCAG CAGAGAAGAT CTGTTTTACG TGGTTGCCTC 
GAACAGCTGC ACAACTATGC AACAGTGGCT CTTCAGTATC CGAAAGCTGT GTTTCAGAAG 
CACCGAATAG AGCAGTGGAA AACTTGGATG GAAGAACTCA TCTGCAACAT GACAATAGAG 
CGCTGTCAGG ATATCTTTAG GAAGTATGAA ATGCAGTATG CTCCGCAGCC ACCTCCAAGT 
GTGTGCCAGT TCATAACCGC GACGGAAATG ACTCTCCAGC GCTATGCAGC AGATATAAAT 
AGCAGACTTA TCAGGCAGGT GGAGCGTCTG AAGCAGGAAG CAGTTACTGT ACCTGTTTGT 
GAAGAGCAGC TGAAAGAAAT CGAACGCTGC ATAAAAGTTT TCCTTCATGA AAATGGAGAG 
GAGGGCTCAC TGAGCTTAGC AAGTGTCATC ATCTCTGCCC TTTGTACGCT GACGAGGCGG 
AATCTGATGA TGGAAGGTGC AGCATCCAGT GCTGGAGAGC AGCTGGTTGA TCTGACTTCG 
AGAGATGGAG CATGGTTTTT GGAGGAGCTC TGCAGCATGA GTGGGAATGT TACTTGCCTT 
GTGCAGTTGC TGAAGCAGTG TCATCTTGTA CCCCAGGACT TAGATATTCC TAACCCAATT 
GAAGCATCAG AAGCTGTTCA TTTAGCTAAT GGAGTATACA TCTCACTACA GGAACTGAAT 
TCCAACTTCC GGCAGATTAT TTTCCCTGAA GCACTTCGGT GCTTAATGAA AGGAGAATAC 
ACTTTAGAAA GTATGCTCCA TGAATTAGAT AACCTTATTG AACAAACAAC TGATGGAGTT 
TCTTTGCAAA CATTGGTTGA ATCTCTTCAG GCCTACTTAA GAAATGCAGC TATGGGACTA 
GAGGAAGAAA CACACACTCA TTACATTGAT GTTGCAAGAG TACTTCATGC TCAGTATGGC 
GAGCTGATTC AGCCAAGGAA TGGTTCTGTG GATGAAACTC CCAAAATGTC AGCTGGTCAG 
ATGCTTTTGG TAGCCTTTGA TGGAATGTTT GCTCAGGTGG AAACTGCGTT TGGTTTATTG 
ATTGAAAAGC TAAACAAGAT GGAAGTCCCT GTTGCTTGGC GTAAGATCGA CATCATTAGG 
GAAGCCCGCA GCACTCAAGT TAACTTCTTT GATGATGACA ATAATCGCCA AGTTCTGGAG 
GAAATCTTCT TTTTGAAGAG ACTGCAAACA ATTAAAGAAT TTTTCAGACT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG AATAAGTTCA CTTGATGACC AGAATGCAGT AAACGGACCT 
GTTCAGATTG TCAATGTGAA AGCCCTTTAC AGAAACTCTT GCTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCTTT TACCGCTGAC TTTGTGAGGC AGCTTTTAAT TGGCCTTCCA 
AATCAGGCTT TAGGACTGAC TTTGTGCAGT TTCATCAGCG CTTTGGGCGT GGATATCATA 
GCTCAGGTAG AAGCCAAAGA CTTTGGAGCA GAAAGCAAAG TTTCTGTGGA TGACCTGTGC 
AAGAAAGCTG TGGAGCACAA TATCCAGATT GGCAAGTTCT CACAGCTGGT CATGAACAGG 
GCAACAGTGC TAGCTAGCTC GTATGATACA GCGTGGAAGA AGCATGATCT GGTGCGCAGA 
CTCGAAACCA GTATTTCTTC TTGCAAGACC AGTCTTCAGA GAGTGCAGCT GCATATTGCC 
ATGTTCCAAT GGCAGCATGA AGATCTCCTT ATCAGTCGCC CACAAGCCAT TTCTGTTACT 
CCTCCACCTC GTTCAGCAAT TTTAGCCAAC ATGAAGAAGA AACTTCATAC GCTCAGCCAG 
ATTGAAGCTT CAATTGCAAC AGTTCAGGAG AAACTAGCAG CACTTGAAGC AAGCATTGAA 
CAGCGTTTGA AATGGGCAGG AGGAGCTAAT CCTGCACTGG CACCAGTCTT GCAAGATTTC 
GATGCCACTA TTGCTGAAAG AAGAAGCCTT GTCCTGAAAG AAAGTCAAAG AGCAAGTCAG 
GTTACTTTCC TGTGCAGTAA TATCATCCAC TTTGAAAGTT TACGTACTAG AACAGCAGAA 
GCCTTAAATC TTGATGCTGC ATTATTTGAA CTTCTCAAAC GCTGTCAACA AATGTGTTCA 
TTTGCATCAC AGTTCAACAG CTCAGTGTCT GAATTGGAGC TTCGTCTGCT GCAGAGAGTG 
GGCACTGGTC TTGAACATCC TATTGGCAGC TCTGAGTGGC TTCACTCAGC CCACAAGCAG 
TTGACACAGG AAATTACTAC ACAGAGAACT GTACAGGCAG AAAGAGAACA GCAAATAGAA 
ACAGTTTGTG AAACTATTCA AAATCTGGTG GATAATATTA AAACTGTGCT CACAGGTCAT 
AATAGACAAC TTGGAGATGT CAAGCATCTA CTGAAGGCTA TGGCAAAGGA TGAAGAAGCA 
GCTTTGGCAG ATGGTGAAGA TGTTCCCTAT GAAAATAGTG TTAGGCAGTT CTTGGGCGAA 
TATAAATCAT GGCAAGATAA TATTCAGACA GTTTTGTTTA CATTAGTTCA GGTTATGGGT 
CAAGTCCGCA ATCAGGAACA TGTTGAAATG CTGCAGGAAA TAACCCCCAC CCTGAAAGAG 
CTAAAAACAC AGAGCCAAAG TGTCTACAAT AATCTAGTGA GTTTTGCATC ACCCTTAGTC 
ACGGATACAT CAAATGAATG CTCAAGCCCA ACATCAGCTG CTACATACCA GCCAACCTTT 
GCTGCAGCTG TACGTAGCAA TACAGGGCAG AAAACTCAGC CAGAGGTGAT GTCACAGAAT 
GCAAGAAAGC TAATTCAGAA AAATCTTGCC ACATCAGCAG ATACTCCTCC AAGCACAGTC 
CCAGGAACTG GCAAAAGTGT TGCCTGTAGT CCCAAGAAGA CAGCCAGGGA CCCTAAAACA 
GGAAAAGCTG TGCAGGAAAG GAATTCATAT GCAGTTAGTG TGTGGAAACG AGTCAAAGCC 
AAGTTGGAGG GCCGAGATGT GGATCCCAGC AGGAGAATGT CTGTTGCTGA ACAGGTTGAC 
TTTGTGATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACT 
GCCTGGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RP11-345J4.5 ( XM_414907.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_414907.4 Gallus gallus Uncharacterized protein (RP11-345J4.5), transcript variant X1, mRNA.

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CloneID	OGa42447
Clone ID Related Accession (Same CDS sequence)	XM_414907.5
Accession Version	XM_414907.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_414907.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCGGCACCG GCAGCAGGCA GCACTACCAC 
CCGCGGGGCT GGAATGACTG GCAACCCAGA ACTGACAGTG CATCAGCTGA CCAGGACAAT 
TTAAAATACT CTTCATCCAG AGATCGGGGC AATTCTGCCT CCTATGGATT ACAACCCTCA 
AATTCAGCTG TGGTGTCTCG ACAAAGACAC GATGATATCA GAGTCCACAC TGACATACAG 
AATGAAGACA AAGGTGGCTA CAGTGTCAAT GGAGGATCTG GGGAAAATAC TTATGGTCGG 
AAGTCGTTGG GGCAAGAGCT GAGAGTTAAC AATGTGACCA ATCCTGATTT TACCAGTGTT 
CCGCATGGGA ATCGTGCTTT AGCCACAAAA GACATGAGGA AATCACAGGA GCGATCGTTG 
TCTTATTCTG ACGAGTCTCG ACTGTCAAAT CTTCTTCGGA GGATTACTCG GGAAGACGAC 
AGAGACCGAA GACTGGCTAC TGTAAAGCAG CTGAAAGAAT TCATTCAGCA ACCAGAAAAC 
AAACTGGTAC TAGTTAAACA ACTGGATAAT ATCCTGACTG CCATTCATGA TGTACTCAAT 
GAAAGTAGCA AATTGCTTCA AGAACTGAGA CAGGAAGGAG CTTGCTGTCT TGGCCTTCTT 
TGTGCTTCTC TAAGCTATGA GGCTGAGAAG ATATTTAAAT GGATTTTTAG CAAATTCAGC 
TTATCCACAA AAGATGAAGT TAAACTTCTT TATTTGTGTG CAACCTACAA AGCACTGGAG 
ACTGTAGGAG AAAAGAAAGC CTTTTCATCT TTAATGCAGC TTGTAATGAC CAGCCTACAG 
TCCATTCTAG AAAATGTGGA TACACCAGAG TTACTGTGCA AATGTGTCAA GTGCATTCTT 
CTGTTGTCCC GATGTTATCC GCACATTTTC AGCACCAATT TTCGGGACAC AGTGGACATA 
CTGGTTGGGT GGCATATAGA TCACACTCAG AAGCCTTCAT TGACTCAGCA GGTGTCCGGG 
TGGCTGCAGA GCTTGGAGCC CTTTTGGGTG GCTGATCTCA CTTTTTCTAC CACACTCTTG 
GGTCAATTTT TAGAAGATAT GGAAGCTTAT GCTGAGGACC TCAGCCATGT GGCTTCTGGG 
GAGTCCCTAG ATGAAGATAT TCCTCCTCCC TCTGTATCAC TACCTAAACT GGCTGCGCTT 
CTTCGAGTTT TCAGTACTGT GGTGAGAAGT ATTGGGGAGC GCTTCAGCCC AATTAGAGGA 
CCACCGATTA CAGAAGCATA TGTAACTGAT GTTCTGTACA GAGTAATGAG ATGTGTGACT 
GCAGCAAACC AAGTATTCTT TTCAGAAGCA GTACTCACAG CTGCCAATGA GTGTGTTGGT 
GTTTTACTTG GCAGTCTGGA TCCAAGCATG ACCATTCACT GTGACATGGT TATCACCTAC 
GGATTGGATC AAATGGAAAA TTGTCAGACT TGTGGCACTG ATTATATCAT TTCAGTCCTG 
AATCTGTTGA CGCTGATTGT TGAACAAATA AATACAAAAC TACCATCGTC ATTTGTAGAG 
AAATTATTTA TTCCAGAGTC TAAACTGCTT GTACTTCGTT ATCATAAGGA AAAAGAGGTT 
GTTGCAGCAG CCCACGCTGT GTATCAGGCA GTACTGAGCC TGAAGAACAT TCCTGTTTTG 
GAGACTGCTT ACAGGTTGAT TCTAGGAGAA ATGACCTGTG CTCTAAACAG TCTTCTTTAT 
AGTTTACATC TTCCCGAGGC CTGTTCTGAA ATCCAGCATG ACTCTTTCAA GAAGCATGTA 
TTCACTGTGG AAAATGCAAA GTTTGTTGTT ATATTTGACC TCAGTGCCCT AAGTACTATT 
GGAAATGCTA AGAACTCATT AATTGGGATG TGGGCCCTTT CGCCAACTGT GTTTGCACTA 
CTGAGTAAAA ATCTGATGAT TGTTCATGGT GACATAGCAG TTCATTTTCC TGCTGTCCAG 
TATGCAGTAC TCTATACGTT GTATTCGCAT TGTTCCAGAC ATGACCATTT TATATCCAGT 
AGCCTCAGTT CTTCCTCTCC TTCCCTGTTC GATGGTGCAG TTATTAGTAC TGTAACCACA 
GCAACAAAAA AACATTTCTC AATCATACTG AACCTTTTGG GGCTACTGTT GAAGAAGGAT 
AACCTTACTC AAGATACCAG GAAACTGCTT ATGACGTGGG CTTTGGAAGT GGCTGTTCTG 
ATGAAGAAAT CAGAGACATA TGCACCCTTA TTTTCTCTCC CATCTTTCCA TAAGTTTTGC 
AAAGGACTTC TAGCAAACAC CCTCCTGGAA GATGTGAATA TTTGTCTTCA AGCGTGTAGC 
AGCCTCCATG CCCTGTCCTC CTCCCTACCA GATGACCTTT TACAGAGATG TGTTGATGTG 
TGTCGTGTTC AACTAGTTCA TAGCGGTGCT CGTGTAAGGC AGACTTTTGG AAAACTTCTG 
AAGTCAATTC CCTTAGATGT GGTGTTGAGC AACCGTAACC ACACAGAAAT ACAAGAAATT 
TCTTTGGCTA TAAGAAGTCA TATGAGCAAA GCTCCAAGTA ATACATTCCA CCCACAGGAT 
TTCTCTGATG TCATTAGTTT TATTTTGTAT GGAAGCACTC ATCGAACTGG GAAAGATAAT 
TGGTTGGAAA GGTTATTCTA TAGCTGTCAA AGGCTGGATA AACGAGATCA ACCAACCATC 
CCTCGTAATC TGTTGAAGAC TGATGCTGTT CTCTGGCAAT GGGCTGTTTG GGAAGCAGCT 
CAGTTTACTG TTCTTTCTAA GCTGAGAACT CCTCTGGGTA GAGCCCAAGA TACATTTCAA 
ACAATTGAAG GTATTATTAG GAGTCTTGCA GCGCATACGC TAAACCCTGA TCAAGATGTT 
AGCCAGTGGA CTACTGCAGA CAATGATGAG GGACACAGCA GCAATCAACT TAGGCTTGTT 
CTCCTTCTAC AGTATCTGGA GAACTTGGAA AAACTGATGT ACAATGCTTA TGAAGGATGT 
GCCAATGCCC TTACCTCTCC ACCCAAGGTT ATCAGGACAT TCTTTTATAC TAATCGCCAG 
ACGTGCCAAG ATTGGCTGAC GCGGATTAGA CTTTCCATCA TGAGAGTTGG ACTGCTGGCC 
GGCCAGCCTG CTGTGACAGT CAGACATGGT TTTGACTTAC TCACAGAAAT GAAAACTAAT 
AACAGTTTAT CTCAGGGAAA TGAATTGGAA GTGACAATTA TGATGCTGGT GGAAGCATTA 
TGTGAACTTC ACTGCCCTGA AGCTATTCAG GGTATTGCTG TCTGGTCTTC TGCTGTAGTC 
GGGAAGAGTC TTACCTGGAT CAACTCTGTA GCTCAACAGG CAGAAGGGCG GTTTGAAAAA 
GCAACTGCGG AGTACCAGGA GCACCTGTGT GCCATGACAG GAGTAGATTG CTGTATATCG 
GGATTTGACA AAACTGTTCT GAAGTTGGCC AATTCCAACA GTGTGAATAA CGCCAGCCCA 
AAGCACTCCT TGAACGGAGA AACTAGAAAA ACTGTTCTGA CTAAGCCAAG TGACTGTTCA 
CCTGAAGTTA TAAATTACTT GGGTAACAAG GCATGTGAAT GTTACATTTC AATTGCTGAT 
TGGTCTGCTG TTCAGGAATG GCAAAATATG GTCCATGACT TGAAGAAAAG CACGAGTAAT 
ACCTCAATCA ATCTGAAAGC AGATTTTAAC TACATCAAAT CCCTGAGCAG CTTTGAGTCT 
GGTGAACTTA CTGAATGCAC TGAACAACTG GAATTATTAC CAGGAGAAAA TATCAACCTA 
CTTGCAGGGG GATCCAAAGA AAAAATAGAC ATGAAGAAGC TCCTTCCTAA TATGCTAAGT 
CCTGACCCGA GAGAACTTCA AAAAGCAGTT GAAGTACAAC TTCTGAGAAG TTCGGTGTGT 
CTGGCAACAG CTGTAAACCA CATGGAGCAG GAGCAAAAGT GGCAGTCAAT ATCTGAAAAT 
GTTATAAAAT ACTTAAAGCA AACATCACGA ATAGCTATTG GGCCTTTAAG ACTTTCCACG 
CTCACCGTGT CTCAGTCTTT ACCAGTGTTG AGCACTCTTC AGTTGTATTG TTCTTCTGCT 
TTAGAAAACA CCGTATCCAA CAGGCTGACA TCAGAGGACT GTCTTATACC TCTCTTCAAT 
GATGCTCTGA GGTCATGTAA ACAGCATGAT GTGAGGCCGT GGATGCATGC ACTGAGATAC 
ACGGTGTATC AGAATCAACT GATGGGAAAG CTTAAAGAAC CAACAGTCCC AATTAAGAGT 
CATCTAATGG AGCTGGGCTT AACAGCAGCA AAATTTGCAC GGAAGCGAGG AAATATTGCC 
CTGGCTACAC GGTTGCTTGC CCAGTGCAGT GAAGTTCAGC TAGGAAAAAC CACCACTGCA 
CAGGACTTAG TCCAGCACTT TAAGAAACTG TCTGCACCAG GTCAGATTGA TGAAAAGTGG 
GGTCCTGAAC TTGATATTGA GAAAACAAAG CTGTTATACA CAGCAGGTCA GTCAACACAT 
GCCATGGAAA TGCTGAGTTC TTGTGCTATA TCATTTTGCA AATCTGCCAA GGCTGAATAT 
GCAGTTGCTA AATCTATTCT TACGCTGGCT AAATGGATTC AAGCAGAATG GAAAGAGATT 
TCAGGACAGT TAAAACAAGT ATATAGAGCT CGTCAACAGC AGAATCACTC TGGTCTGTCT 
ACCTTGTCTA AGAACATATA TACTTTGATT GATCTCCCAG CTGTTAATAC GTTAGAAGAG 
GAATATCCCA GGATTGAAAG TGAATCTACA GTAAATATTG GAGTTGGAGA ACCTGACTTT 
ATCTTGGGAC AGCTGTATCA CTTGTCTTCA GTACAGGCAC CTGAAGTGGC CAAGTCTTGG 
GCAGCCCTGG CTAGTTGGGC TTATAGATGG GGCCGGAAAG TAGTTGATAA TGCCAGTCAG 
GGAGAAGGTG TTCGTCTGCT GCCCCGAGAG AAATCTGAGG TTCAGAGCTT GCTTCCAGAC 
AATGTTACAG AGGAAGACAA AGAGAAGATT TATGGGATCC TTGGGCAAGC AGTGTGTCGT 
CCAGCTGGGA TTCAAGATGA AGATATGACT CTGCAAATCA CTGAAAATGA AGACAATGAA 
GAAGATGATA TGGTGGATGT TATATGGCGT CAGTTATTAA CAACTTGTCC CTGGCTTTCA 
GATCTTGATG AAAATGCAAC AGAAGGTTTA ATTAAAGTAT GGCGGAAAGT TGTAGACAGA 
ATCTTCAGTC TCTATAAACT CTCCTGCAGT GCATATTTCA CTTTCCTCAA ACTCAATGCT 
GGTCAGGTTC CACTTGATGA GGATGATCCA AGGCTGCATT TAAGAAATAC TTCTGAGCAA 
AGCACTGATG ATGTTATTGT GATGGCAACC CTAAGACTGT TAAGGCTGCT TGTTAAACAT 
GCTGGAGAGC TTAGACAGTA TCTAGAGCAT GGGCTAGAAA CTACACCTAC TGCTCCTTGG 
AGAGGTATTA TTCCTCAGCT CTTCTCTCGC CTCAATCATC CAGAAGTGTA TGTTCGTCAG 
AGTATTTGCA ACTTACTGTG TCGAGTGGCT CAAGATTCTC CTCATCTCAT ACTGTATCCT 
GCAATAGTGG GTACTATTTC ACTCAGCAGT GAATCCCAAA CCTCAGGGAA CAAGCTTCCT 
TCAGCGATTC CCACTTTGCT AGGTAATATT CAAGGAGAAG AACTGTTGGG TGGTGAATGT 
GATGGTGGGA GTCCACCAGC TTCTCAAGAA AGCACTAAAG ATGACACAAA AGTTGGATTA 
AATGAAGATC AAGCAATGAT GCAAGATTGT TACAGCAAAA TTGTGGAGAA ACTCTCAACT 
GCAAATCCAA CAATGGTATT GCAGGTTCAG ATGCTAGTGG CTGAGCTGCG CAGAGTTACA 
GTTCTGTGGG ATGAACTTTG GTTGGGCGTT TTACTGCAGC AGCACATGTA TGTCCTCAGA 
AGGATTCAGC AACTGGAAGA TGAAGTCAAA AGGGTCCAAA ACAACAACAC TCTACGCAAA 
GAGGAGAAGA TAGCAATCAT GCGTGAGAAG CATACAGCTC TAATGAAGCC CATTGTATTT 
GCTTTGGAAC ATGTACGGAG CATCACTGCA GCTCCTACAG AAACTCCTCA TGAGAAATGG 
TTTCAGGATA ACTATGGCGG TGCAATCGAA AATGCACTAG AGAAACTTAA GAATCCTTTG 
AATCCTGCTA AGCCTGGAAG CAGCTGGCTC CCCTTTAAAG AGGTTATGCT AAGTTTGCAA 
CAGAGAGCAC AGAAACGGGC TAGTTATCTT TTACGGCTTG AAGAAATCAG TCCTTGGTTG 
GCTGCCATGA TGAACACTGA AATAGCGCTC CCTGGAGAAG TATCCACCAG AGACACTGTC 
ACAATTCATA GTGTGGGCAG CACCATCACA ATTCTGCCTA CCAAAACCAA ACCTAAAAAG 
CTGCTTTTCC TGGGCTCAGA TGGGAAGAAC TATCCATACT TGTTCAAAGG TTTAGAGGAC 
TTGCACCTAG ATGAAAGAAT CATGCAATTC CTATCAATTG TGAACACAAT GTTTGCTACC 
ATTAACCGTC AAGAGACACC AAGGTTTCAT GCACGGCACT ACTCTGTGAC ACCTCTGGGA 
ACAAGATCAG GCCTGATCCA GTGGGTGGAT GGAGCTACAC CTTTGTTTGG TCTTTACAAA 
CGATGGCAAC AGCGGGAAGC TGCTTTACAA GCACAAAAGG CTCAGGATTC TTATCAGACT 
CCTCAGAATC CTGGAATCGT GCCTAGACCT AGTGAACTGT ACTACAGTAA AATTGGACCT 
GCTTTAAAGG CAGTTGGGCT TAGTCTTGAT GTGTCGCGTC GTGACTGGCC CTTGAATGTG 
ATGAGAGCTG TTCTAGAAGA ACTGATGGAA GCAACTCCCC CAAATCTCCT AGCTAAGGAG 
CTCTGGTCGT CATGTACTAC ACCAGATGAA TGGTGGAGAG TTACACAGTC TTATGCAAGA 
TCCACTGCAG TTATGTCCAT GGTTGGCTAC ATCATTGGTC TTGGAGACAG ACATCTGGAT 
AATGTTCTTA TAGATATGAC TACAGGAGAA GTTGTCCACA TAGATTATAA TGTTTGCTTT 
GAAAAAGGGA AGAGCCTTAG GGTACCAGAG AAGGTTCCAT TCCGAATGAC ACACAATATT 
GAAACAGCAC TTGGAGTGAC AGGAGTAGAG GGGGTTTTCA GGCTTTCTTG TGAACAGGTC 
CTTCATATCA TGCGGCGTGG GAGAGAAACT TTGCTGACGT TGCTTGAAGC TTTTGTTTAT 
GATCCTCTGG TGGACTGGAC AGCAGGGGGT GAGGCAGGAT TTGCAGGAGC TGTGTATGGT 
GGTGGCGGCC AGCAGGCTGA AAACAAGCAA AGCAAAAGAG AAATGGAAAG AGATATTACA 
CGTAGTCTCT TCTCTTCAAG AGTGGCTGAG ATAAAGGTTA ACTGGTTTAA GAACAGAGAT 
GAAATGTTGG CTGTGCTTCC AAAGTTGGAC AGTAGTTTAG AAGAATATCT CAACCTGCAA 
GAACAGTTGA CAGATGTAGA AAAATTGCAA GGAAAGCTAC TGGAGGAGAT AGAATTTCTT 
GAAGGTGCAG AAGGAGTGGA TCATCCCTCT CACACACTTC AGCACAGGTA TTCTGAGCAC 
ACACAGTTGC AGACTCAACA AAGAGCTGTC CAGGAGGCGA TCCAGGTGAA GCTGAATGAA 
TTTGAGCAGT GGATAACACA CTACCAAGCT GCTTTCAGTA ATTTAGAGGC AACACAACTT 
GCAAGTCTCC TTCAAGAAAT TAGCACACAA ATGGACCTAG GTCCTCCGAG TTATGTCCCA 
GCAACAGCAT TTCTGCAAAA TGCAGGCCAG GCACATCTCA TCAGTCAGTG TGAACAATTA 
GAAGGGGAGG TTGGTGCTCT TCTCCAGCAG AGAAGATCTG TTTTACGTGG TTGCCTCGAA 
CAGCTGCACA ACTATGCAAC AGTGGCTCTT CAGTATCCGA AAGCTGTGTT TCAGAAGCAC 
CGAATAGAGC AGTGGAAAAC TTGGATGGAA GAACTCATCT GCAACATGAC AATAGAGCGC 
TGTCAGGATA TCTTTAGGAA GTATGAAATG CAGTATGCTC CGCAGCCACC TCCAAGTGTG 
TGCCAGTTCA TAACCGCGAC GGAAATGACT CTCCAGCGCT ATGCAGCAGA TATAAATAGC 
AGACTTATCA GGCAGGTGGA GCGTCTGAAG CAGGAAGCAG TTACTGTACC TGTTTGTGAA 
GAGCAGCTGA AAGAAATCGA ACGCTGCATA AAAGTTTTCC TTCATGAAAA TGGAGAGGAG 
GGCTCACTGA GCTTAGCAAG TGTCATCATC TCTGCCCTTT GTACGCTGAC GAGGCGGAAT 
CTGATGATGG AAGGTGCAGC ATCCAGTGCT GGAGAGCAGC TGGTTGATCT GACTTCGAGA 
GATGGAGCAT GGTTTTTGGA GGAGCTCTGC AGCATGAGTG GGAATGTTAC TTGCCTTGTG 
CAGTTGCTGA AGCAGTGTCA TCTTGTACCC CAGGACTTAG ATATTCCTAA CCCAATTGAA 
GCATCAGAAG CTGTTCATTT AGCTAATGGA GTATACATCT CACTACAGGA ACTGAATTCC 
AACTTCCGGC AGATTATTTT CCCTGAAGCA CTTCGGTGCT TAATGAAAGG AGAATACACT 
TTAGAAAGTA TGCTCCATGA ATTAGATAAC CTTATTGAAC AAACAACTGA TGGAGTTTCT 
TTGCAAACAT TGGTTGAATC TCTTCAGGCC TACTTAAGAA ATGCAGCTAT GGGACTAGAG 
GAAGAAACAC ACACTCATTA CATTGATGTT GCAAGAGTAC TTCATGCTCA GTATGGCGAG 
CTGATTCAGC CAAGGAATGG TTCTGTGGAT GAAACTCCCA AAATGTCAGC TGGTCAGATG 
CTTTTGGTAG CCTTTGATGG AATGTTTGCT CAGGTGGAAA CTGCGTTTGG TTTATTGATT 
GAAAAGCTAA ACAAGATGGA AGTCCCTGTT GCTTGGCGTA AGATCGACAT CATTAGGGAA 
GCCCGCAGCA CTCAAGTTAA CTTCTTTGAT GATGACAATA ATCGCCAAGT TCTGGAGGAA 
ATCTTCTTTT TGAAGAGACT GCAAACAATT AAAGAATTTT TCAGACTCTG TGGTACCTTT 
TCTAAAACAT TGTCAGGAAT AAGTTCACTT GATGACCAGA ATGCAGTAAA CGGACCTGTT 
CAGATTGTCA ATGTGAAAGC CCTTTACAGA AACTCTTGCT TCAGTGAAGA CCAAATGGCC 
AAACCTATCA AGGCTTTTAC CGCTGACTTT GTGAGGCAGC TTTTAATTGG CCTTCCAAAT 
CAGGCTTTAG GACTGACTTT GTGCAGTTTC ATCAGCGCTT TGGGCGTGGA TATCATAGCT 
CAGGTAGAAG CCAAAGACTT TGGAGCAGAA AGCAAAGTTT CTGTGGATGA CCTGTGCAAG 
AAAGCTGTGG AGCACAATAT CCAGATTGGC AAGTTCTCAC AGCTGGTCAT GAACAGGGCA 
ACAGTGCTAG CTAGCTCGTA TGATACAGCG TGGAAGAAGC ATGATCTGGT GCGCAGACTC 
GAAACCAGTA TTTCTTCTTG CAAGACCAGT CTTCAGAGAG TGCAGCTGCA TATTGCCATG 
TTCCAATGGC AGCATGAAGA TCTCCTTATC AGTCGCCCAC AAGCCATTTC TGTTACTCCT 
CCACCTCGTT CAGCAATTTT AGCCAACATG AAGAAGAAAC TTCATACGCT CAGCCAGATT 
GAAGCTTCAA TTGCAACAGT TCAGGAGAAA CTAGCAGCAC TTGAAGCAAG CATTGAACAG 
CGTTTGAAAT GGGCAGGAGG AGCTAATCCT GCACTGGCAC CAGTCTTGCA AGATTTCGAT 
GCCACTATTG CTGAAAGAAG AAGCCTTGTC CTGAAAGAAA GTCAAAGAGC AAGTCAGGTT 
ACTTTCCTGT GCAGTAATAT CATCCACTTT GAAAGTTTAC GTACTAGAAC AGCAGAAGCC 
TTAAATCTTG ATGCTGCATT ATTTGAACTT CTCAAACGCT GTCAACAAAT GTGTTCATTT 
GCATCACAGT TCAACAGCTC AGTGTCTGAA TTGGAGCTTC GTCTGCTGCA GAGAGTGGGC 
ACTGGTCTTG AACATCCTAT TGGCAGCTCT GAGTGGCTTC ACTCAGCCCA CAAGCAGTTG 
ACACAGGAAA TTACTACACA GAGAACTGTA CAGGCAGAAA GAGAACAGCA AATAGAAACA 
GTTTGTGAAA CTATTCAAAA TCTGGTGGAT AATATTAAAA CTGTGCTCAC AGGTCATAAT 
AGACAACTTG GAGATGTCAA GCATCTACTG AAGGCTATGG CAAAGGATGA AGAAGCAGCT 
TTGGCAGATG GTGAAGATGT TCCCTATGAA AATAGTGTTA GGCAGTTCTT GGGCGAATAT 
AAATCATGGC AAGATAATAT TCAGACAGTT TTGTTTACAT TAGTTCAGGT TATGGGTCAA 
GTCCGCAATC AGGAACATGT TGAAATGCTG CAGGAAATAA CCCCCACCCT GAAAGAGCTA 
AAAACACAGA GCCAAAGTGT CTACAATAAT CTAGTGAGTT TTGCATCACC CTTAGTCACG 
GATACATCAA ATGAATGCTC AAGCCCAACA TCAGCTGCTA CATACCAGCC AACCTTTGCT 
GCAGCTGTAC GTAGCAATAC AGGGCAGAAA ACTCAGCCAG AGGTGATGTC ACAGAATGCA 
AGAAAGCTAA TTCAGAAAAA TCTTGCCACA TCAGCAGATA CTCCTCCAAG CACAGTCCCA 
GGAACTGGCA AAAGTGTTGC CTGTAGTCCC AAGAAGACAG CCAGGGACCC TAAAACAGGA 
AAAGCTGTGC AGGAAAGGAA TTCATATGCA GTTAGTGTGT GGAAACGAGT CAAAGCCAAG 
TTGGAGGGCC GAGATGTGGA TCCCAGCAGG AGAATGTCTG TTGCTGAACA GGTTGACTTT 
GTGATTAAGG AAGCAACTAA TCTAGATAAC TTGGCTCAGC TGTATGAAGG TTGGACTGCC 
TGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_414907.4
CDS	93..11081
Translation	MSRRAPGSRLSGTGSRQHYHPRGWNDWQPRTDSASADQDNLKYSSSRDRGNSASYGLQPSNSAVVSRQRHDDIRVHTDIQNEDKGGYSVNGGSGENTYGRKSLGQELRVNNVTNPDFTSVPHGNRALATKDMRKSQERSLSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILTAIHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSLSTKDEVKLLYLCATYKALETVGEKKAFSSLMQLVMTSLQSILENVDTPELLCKCVKCILLLSRCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLTFSTTLLGQFLEDMEAYAEDLSHVASGESLDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQMENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPESKLLVLRYHKEKEVVAAAHAVYQAVLSLKNIPVLETAYRLILGEMTCALNSLLYSLHLPEACSEIQHDSFKKHVFTVENAKFVVIFDLSALSTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDIAVHFPAVQYAVLYTLYSHCSRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGLLLKKDNLTQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLLEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGARVRQTFGKLLKSIPLDVVLSNRNHTEIQEISLAIRSHMSKAPSNTFHPQDFSDVISFILYGSTHRTGKDNWLERLFYSCQRLDKRDQPTIPRNLLKTDAVLWQWAVWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNNSLSQGNELEVTIMMLVEALCELHCPEAIQGIAVWSSAVVGKSLTWINSVAQQAEGRFEKATAEYQEHLCAMTGVDCCISGFDKTVLKLANSNSVNNASPKHSLNGETRKTVLTKPSDCSPEVINYLGNKACECYISIADWSAVQEWQNMVHDLKKSTSNTSINLKADFNYIKSLSSFESGELTECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKAVEVQLLRSSVCLATAVNHMEQEQKWQSISENVIKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLTSEDCLIPLFNDALRSCKQHDVRPWMHALRYTVYQNQLMGKLKEPTVPIKSHLMELGLTAAKFARKRGNIALATRLLAQCSEVQLGKTTTAQDLVQHFKKLSAPGQIDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRARQQQNHSGLSTLSKNIYTLIDLPAVNTLEEEYPRIESESTVNIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQSLLPDNVTEEDKEKIYGILGQAVCRPAGIQDEDMTLQITENEDNEEDDMVDVIWRQLLTTCPWLSDLDENATEGLIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVPLDEDDPRLHLRNTSEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQTSGNKLPSAIPTLLGNIQGEELLGGECDGGSPPASQESTKDDTKVGLNEDQAMMQDCYSKIVEKLSTANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPTETPHEKWFQDNYGGAIENALEKLKNPLNPAKPGSSWLPFKEVMLSLQQRAQKRASYLLRLEEISPWLAAMMNTEIALPGEVSTRDTVTIHSVGSTITILPTKTKPKKLLFLGSDGKNYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLNVMRAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLAVLPKLDSSLEEYLNLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFSNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHNYATVALQYPKAVFQKHRIEQWKTWMEELICNMTIERCQDIFRKYEMQYAPQPPPSVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEEQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPIEASEAVHLANGVYISLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDNLIEQTTDGVSLQTLVESLQAYLRNAAMGLEEETHTHYIDVARVLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLIEKLNKMEVPVAWRKIDIIREARSTQVNFFDDDNNRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGISSLDDQNAVNGPVQIVNVKALYRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLISRPQAISVTPPPRSAILANMKKKLHTLSQIEASIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFDATIAERRSLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELLKRCQQMCSFASQFNSSVSELELRLLQRVGTGLEHPIGSSEWLHSAHKQLTQEITTQRTVQAEREQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQVMGQVRNQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDTSNECSSPTSAATYQPTFAAAVRSNTGQKTQPEVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKTARDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPSRRMSVAEQVDFVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_414907.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Uncharacterized protein (RP11-345J4.5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_414907.5

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCGGCACCG GCAGCAGGCA GCACTACCAC 
CCGCGGGGCT GGAATGACTG GCAACCCAGA ACTGACAGTG CATCAGCTGA CCAGGACAAT 
TTAAAATACT CTTCATCCAG AGATCGGGGC AATTCTGCCT CCTATGGATT ACAACCCTCA 
AATTCAGCTG TGGTGTCTCG ACAAAGACAC GATGATATCA GAGTCCACAC TGACATACAG 
AATGAAGACA AAGGTGGCTA CAGTGTCAAT GGAGGATCTG GGGAAAATAC TTATGGTCGG 
AAGTCGTTGG GGCAAGAGCT GAGAGTTAAC AATGTGACCA ATCCTGATTT TACCAGTGTT 
CCGCATGGGA ATCGTGCTTT AGCCACAAAA GACATGAGGA AATCACAGGA GCGATCGTTG 
TCTTATTCTG ACGAGTCTCG ACTGTCAAAT CTTCTTCGGA GGATTACTCG GGAAGACGAC 
AGAGACCGAA GACTGGCTAC TGTAAAGCAG CTGAAAGAAT TCATTCAGCA ACCAGAAAAC 
AAACTGGTAC TAGTTAAACA ACTGGATAAT ATCCTGACTG CCATTCATGA TGTACTCAAT 
GAAAGTAGCA AATTGCTTCA AGAACTGAGA CAGGAAGGAG CTTGCTGTCT TGGCCTTCTT 
TGTGCTTCTC TAAGCTATGA GGCTGAGAAG ATATTTAAAT GGATTTTTAG CAAATTCAGC 
TTATCCACAA AAGATGAAGT TAAACTTCTT TATTTGTGTG CAACCTACAA AGCACTGGAG 
ACTGTAGGAG AAAAGAAAGC CTTTTCATCT TTAATGCAGC TTGTAATGAC CAGCCTACAG 
TCCATTCTAG AAAATGTGGA TACACCAGAG TTACTGTGCA AATGTGTCAA GTGCATTCTT 
CTGTTGTCCC GATGTTATCC GCACATTTTC AGCACCAATT TTCGGGACAC AGTGGACATA 
CTGGTTGGGT GGCATATAGA TCACACTCAG AAGCCTTCAT TGACTCAGCA GGTGTCCGGG 
TGGCTGCAGA GCTTGGAGCC CTTTTGGGTG GCTGATCTCA CTTTTTCTAC CACACTCTTG 
GGTCAATTTT TAGAAGATAT GGAAGCTTAT GCTGAGGACC TCAGCCATGT GGCTTCTGGG 
GAGTCCCTAG ATGAAGATAT TCCTCCTCCC TCTGTATCAC TACCTAAACT GGCTGCGCTT 
CTTCGAGTTT TCAGTACTGT GGTGAGAAGT ATTGGGGAGC GCTTCAGCCC AATTAGAGGA 
CCACCGATTA CAGAAGCATA TGTAACTGAT GTTCTGTACA GAGTAATGAG ATGTGTGACT 
GCAGCAAACC AAGTATTCTT TTCAGAAGCA GTACTCACAG CTGCCAATGA GTGTGTTGGT 
GTTTTACTTG GCAGTCTGGA TCCAAGCATG ACCATTCACT GTGACATGGT TATCACCTAC 
GGATTGGATC AAATGGAAAA TTGTCAGACT TGTGGCACTG ATTATATCAT TTCAGTCCTG 
AATCTGTTGA CGCTGATTGT TGAACAAATA AATACAAAAC TACCATCGTC ATTTGTAGAG 
AAATTATTTA TTCCAGAGTC TAAACTGCTT GTACTTCGTT ATCATAAGGA AAAAGAGGTT 
GTTGCAGCAG CCCACGCTGT GTATCAGGCA GTACTGAGCC TGAAGAACAT TCCTGTTTTG 
GAGACTGCTT ACAGGTTGAT TCTAGGAGAA ATGACCTGTG CTCTAAACAG TCTTCTTTAT 
AGTTTACATC TTCCCGAGGC CTGTTCTGAA ATCCAGCATG ACTCTTTCAA GAAGCATGTA 
TTCACTGTGG AAAATGCAAA GTTTGTTGTT ATATTTGACC TCAGTGCCCT AAGTACTATT 
GGAAATGCTA AGAACTCATT AATTGGGATG TGGGCCCTTT CGCCAACTGT GTTTGCACTA 
CTGAGTAAAA ATCTGATGAT TGTTCATGGT GACATAGCAG TTCATTTTCC TGCTGTCCAG 
TATGCAGTAC TCTATACGTT GTATTCGCAT TGTTCCAGAC ATGACCATTT TATATCCAGT 
AGCCTCAGTT CTTCCTCTCC TTCCCTGTTC GATGGTGCAG TTATTAGTAC TGTAACCACA 
GCAACAAAAA AACATTTCTC AATCATACTG AACCTTTTGG GGCTACTGTT GAAGAAGGAT 
AACCTTACTC AAGATACCAG GAAACTGCTT ATGACGTGGG CTTTGGAAGT GGCTGTTCTG 
ATGAAGAAAT CAGAGACATA TGCACCCTTA TTTTCTCTCC CATCTTTCCA TAAGTTTTGC 
AAAGGACTTC TAGCAAACAC CCTCCTGGAA GATGTGAATA TTTGTCTTCA AGCGTGTAGC 
AGCCTCCATG CCCTGTCCTC CTCCCTACCA GATGACCTTT TACAGAGATG TGTTGATGTG 
TGTCGTGTTC AACTAGTTCA TAGCGGTGCT CGTGTAAGGC AGACTTTTGG AAAACTTCTG 
AAGTCAATTC CCTTAGATGT GGTGTTGAGC AACCGTAACC ACACAGAAAT ACAAGAAATT 
TCTTTGGCTA TAAGAAGTCA TATGAGCAAA GCTCCAAGTA ATACATTCCA CCCACAGGAT 
TTCTCTGATG TCATTAGTTT TATTTTGTAT GGAAGCACTC ATCGAACTGG GAAAGATAAT 
TGGTTGGAAA GGTTATTCTA TAGCTGTCAA AGGCTGGATA AACGAGATCA ACCAACCATC 
CCTCGTAATC TGTTGAAGAC TGATGCTGTT CTCTGGCAAT GGGCTGTTTG GGAAGCAGCT 
CAGTTTACTG TTCTTTCTAA GCTGAGAACT CCTCTGGGTA GAGCCCAAGA TACATTTCAA 
ACAATTGAAG GTATTATTAG GAGTCTTGCA GCGCATACGC TAAACCCTGA TCAAGATGTT 
AGCCAGTGGA CTACTGCAGA CAATGATGAG GGACACAGCA GCAATCAACT TAGGCTTGTT 
CTCCTTCTAC AGTATCTGGA GAACTTGGAA AAACTGATGT ACAATGCTTA TGAAGGATGT 
GCCAATGCCC TTACCTCTCC ACCCAAGGTT ATCAGGACAT TCTTTTATAC TAATCGCCAG 
ACGTGCCAAG ATTGGCTGAC GCGGATTAGA CTTTCCATCA TGAGAGTTGG ACTGCTGGCC 
GGCCAGCCTG CTGTGACAGT CAGACATGGT TTTGACTTAC TCACAGAAAT GAAAACTAAT 
AACAGTTTAT CTCAGGGAAA TGAATTGGAA GTGACAATTA TGATGCTGGT GGAAGCATTA 
TGTGAACTTC ACTGCCCTGA AGCTATTCAG GGTATTGCTG TCTGGTCTTC TGCTGTAGTC 
GGGAAGAGTC TTACCTGGAT CAACTCTGTA GCTCAACAGG CAGAAGGGCG GTTTGAAAAA 
GCAACTGCGG AGTACCAGGA GCACCTGTGT GCCATGACAG GAGTAGATTG CTGTATATCG 
GGATTTGACA AAACTGTTCT GAAGTTGGCC AATTCCAACA GTGTGAATAA CGCCAGCCCA 
AAGCACTCCT TGAACGGAGA AACTAGAAAA ACTGTTCTGA CTAAGCCAAG TGACTGTTCA 
CCTGAAGTTA TAAATTACTT GGGTAACAAG GCATGTGAAT GTTACATTTC AATTGCTGAT 
TGGTCTGCTG TTCAGGAATG GCAAAATATG GTCCATGACT TGAAGAAAAG CACGAGTAAT 
ACCTCAATCA ATCTGAAAGC AGATTTTAAC TACATCAAAT CCCTGAGCAG CTTTGAGTCT 
GGTGAACTTA CTGAATGCAC TGAACAACTG GAATTATTAC CAGGAGAAAA TATCAACCTA 
CTTGCAGGGG GATCCAAAGA AAAAATAGAC ATGAAGAAGC TCCTTCCTAA TATGCTAAGT 
CCTGACCCGA GAGAACTTCA AAAAGCAGTT GAAGTACAAC TTCTGAGAAG TTCGGTGTGT 
CTGGCAACAG CTGTAAACCA CATGGAGCAG GAGCAAAAGT GGCAGTCAAT ATCTGAAAAT 
GTTATAAAAT ACTTAAAGCA AACATCACGA ATAGCTATTG GGCCTTTAAG ACTTTCCACG 
CTCACCGTGT CTCAGTCTTT ACCAGTGTTG AGCACTCTTC AGTTGTATTG TTCTTCTGCT 
TTAGAAAACA CCGTATCCAA CAGGCTGACA TCAGAGGACT GTCTTATACC TCTCTTCAAT 
GATGCTCTGA GGTCATGTAA ACAGCATGAT GTGAGGCCGT GGATGCATGC ACTGAGATAC 
ACGGTGTATC AGAATCAACT GATGGGAAAG CTTAAAGAAC CAACAGTCCC AATTAAGAGT 
CATCTAATGG AGCTGGGCTT AACAGCAGCA AAATTTGCAC GGAAGCGAGG AAATATTGCC 
CTGGCTACAC GGTTGCTTGC CCAGTGCAGT GAAGTTCAGC TAGGAAAAAC CACCACTGCA 
CAGGACTTAG TCCAGCACTT TAAGAAACTG TCTGCACCAG GTCAGATTGA TGAAAAGTGG 
GGTCCTGAAC TTGATATTGA GAAAACAAAG CTGTTATACA CAGCAGGTCA GTCAACACAT 
GCCATGGAAA TGCTGAGTTC TTGTGCTATA TCATTTTGCA AATCTGCCAA GGCTGAATAT 
GCAGTTGCTA AATCTATTCT TACGCTGGCT AAATGGATTC AAGCAGAATG GAAAGAGATT 
TCAGGACAGT TAAAACAAGT ATATAGAGCT CGTCAACAGC AGAATCACTC TGGTCTGTCT 
ACCTTGTCTA AGAACATATA TACTTTGATT GATCTCCCAG CTGTTAATAC GTTAGAAGAG 
GAATATCCCA GGATTGAAAG TGAATCTACA GTAAATATTG GAGTTGGAGA ACCTGACTTT 
ATCTTGGGAC AGCTGTATCA CTTGTCTTCA GTACAGGCAC CTGAAGTGGC CAAGTCTTGG 
GCAGCCCTGG CTAGTTGGGC TTATAGATGG GGCCGGAAAG TAGTTGATAA TGCCAGTCAG 
GGAGAAGGTG TTCGTCTGCT GCCCCGAGAG AAATCTGAGG TTCAGAGCTT GCTTCCAGAC 
AATGTTACAG AGGAAGACAA AGAGAAGATT TATGGGATCC TTGGGCAAGC AGTGTGTCGT 
CCAGCTGGGA TTCAAGATGA AGATATGACT CTGCAAATCA CTGAAAATGA AGACAATGAA 
GAAGATGATA TGGTGGATGT TATATGGCGT CAGTTATTAA CAACTTGTCC CTGGCTTTCA 
GATCTTGATG AAAATGCAAC AGAAGGTTTA ATTAAAGTAT GGCGGAAAGT TGTAGACAGA 
ATCTTCAGTC TCTATAAACT CTCCTGCAGT GCATATTTCA CTTTCCTCAA ACTCAATGCT 
GGTCAGGTTC CACTTGATGA GGATGATCCA AGGCTGCATT TAAGAAATAC TTCTGAGCAA 
AGCACTGATG ATGTTATTGT GATGGCAACC CTAAGACTGT TAAGGCTGCT TGTTAAACAT 
GCTGGAGAGC TTAGACAGTA TCTAGAGCAT GGGCTAGAAA CTACACCTAC TGCTCCTTGG 
AGAGGTATTA TTCCTCAGCT CTTCTCTCGC CTCAATCATC CAGAAGTGTA TGTTCGTCAG 
AGTATTTGCA ACTTACTGTG TCGAGTGGCT CAAGATTCTC CTCATCTCAT ACTGTATCCT 
GCAATAGTGG GTACTATTTC ACTCAGCAGT GAATCCCAAA CCTCAGGGAA CAAGCTTCCT 
TCAGCGATTC CCACTTTGCT AGGTAATATT CAAGGAGAAG AACTGTTGGG TGGTGAATGT 
GATGGTGGGA GTCCACCAGC TTCTCAAGAA AGCACTAAAG ATGACACAAA AGTTGGATTA 
AATGAAGATC AAGCAATGAT GCAAGATTGT TACAGCAAAA TTGTGGAGAA ACTCTCAACT 
GCAAATCCAA CAATGGTATT GCAGGTTCAG ATGCTAGTGG CTGAGCTGCG CAGAGTTACA 
GTTCTGTGGG ATGAACTTTG GTTGGGCGTT TTACTGCAGC AGCACATGTA TGTCCTCAGA 
AGGATTCAGC AACTGGAAGA TGAAGTCAAA AGGGTCCAAA ACAACAACAC TCTACGCAAA 
GAGGAGAAGA TAGCAATCAT GCGTGAGAAG CATACAGCTC TAATGAAGCC CATTGTATTT 
GCTTTGGAAC ATGTACGGAG CATCACTGCA GCTCCTACAG AAACTCCTCA TGAGAAATGG 
TTTCAGGATA ACTATGGCGG TGCAATCGAA AATGCACTAG AGAAACTTAA GAATCCTTTG 
AATCCTGCTA AGCCTGGAAG CAGCTGGCTC CCCTTTAAAG AGGTTATGCT AAGTTTGCAA 
CAGAGAGCAC AGAAACGGGC TAGTTATCTT TTACGGCTTG AAGAAATCAG TCCTTGGTTG 
GCTGCCATGA TGAACACTGA AATAGCGCTC CCTGGAGAAG TATCCACCAG AGACACTGTC 
ACAATTCATA GTGTGGGCAG CACCATCACA ATTCTGCCTA CCAAAACCAA ACCTAAAAAG 
CTGCTTTTCC TGGGCTCAGA TGGGAAGAAC TATCCATACT TGTTCAAAGG TTTAGAGGAC 
TTGCACCTAG ATGAAAGAAT CATGCAATTC CTATCAATTG TGAACACAAT GTTTGCTACC 
ATTAACCGTC AAGAGACACC AAGGTTTCAT GCACGGCACT ACTCTGTGAC ACCTCTGGGA 
ACAAGATCAG GCCTGATCCA GTGGGTGGAT GGAGCTACAC CTTTGTTTGG TCTTTACAAA 
CGATGGCAAC AGCGGGAAGC TGCTTTACAA GCACAAAAGG CTCAGGATTC TTATCAGACT 
CCTCAGAATC CTGGAATCGT GCCTAGACCT AGTGAACTGT ACTACAGTAA AATTGGACCT 
GCTTTAAAGG CAGTTGGGCT TAGTCTTGAT GTGTCGCGTC GTGACTGGCC CTTGAATGTG 
ATGAGAGCTG TTCTAGAAGA ACTGATGGAA GCAACTCCCC CAAATCTCCT AGCTAAGGAG 
CTCTGGTCGT CATGTACTAC ACCAGATGAA TGGTGGAGAG TTACACAGTC TTATGCAAGA 
TCCACTGCAG TTATGTCCAT GGTTGGCTAC ATCATTGGTC TTGGAGACAG ACATCTGGAT 
AATGTTCTTA TAGATATGAC TACAGGAGAA GTTGTCCACA TAGATTATAA TGTTTGCTTT 
GAAAAAGGGA AGAGCCTTAG GGTACCAGAG AAGGTTCCAT TCCGAATGAC ACACAATATT 
GAAACAGCAC TTGGAGTGAC AGGAGTAGAG GGGGTTTTCA GGCTTTCTTG TGAACAGGTC 
CTTCATATCA TGCGGCGTGG GAGAGAAACT TTGCTGACGT TGCTTGAAGC TTTTGTTTAT 
GATCCTCTGG TGGACTGGAC AGCAGGGGGT GAGGCAGGAT TTGCAGGAGC TGTGTATGGT 
GGTGGCGGCC AGCAGGCTGA AAACAAGCAA AGCAAAAGAG AAATGGAAAG AGATATTACA 
CGTAGTCTCT TCTCTTCAAG AGTGGCTGAG ATAAAGGTTA ACTGGTTTAA GAACAGAGAT 
GAAATGTTGG CTGTGCTTCC AAAGTTGGAC AGTAGTTTAG AAGAATATCT CAACCTGCAA 
GAACAGTTGA CAGATGTAGA AAAATTGCAA GGAAAGCTAC TGGAGGAGAT AGAATTTCTT 
GAAGGTGCAG AAGGAGTGGA TCATCCCTCT CACACACTTC AGCACAGGTA TTCTGAGCAC 
ACACAGTTGC AGACTCAACA AAGAGCTGTC CAGGAGGCGA TCCAGGTGAA GCTGAATGAA 
TTTGAGCAGT GGATAACACA CTACCAAGCT GCTTTCAGTA ATTTAGAGGC AACACAACTT 
GCAAGTCTCC TTCAAGAAAT TAGCACACAA ATGGACCTAG GTCCTCCGAG TTATGTCCCA 
GCAACAGCAT TTCTGCAAAA TGCAGGCCAG GCACATCTCA TCAGTCAGTG TGAACAATTA 
GAAGGGGAGG TTGGTGCTCT TCTCCAGCAG AGAAGATCTG TTTTACGTGG TTGCCTCGAA 
CAGCTGCACA ACTATGCAAC AGTGGCTCTT CAGTATCCGA AAGCTGTGTT TCAGAAGCAC 
CGAATAGAGC AGTGGAAAAC TTGGATGGAA GAACTCATCT GCAACATGAC AATAGAGCGC 
TGTCAGGATA TCTTTAGGAA GTATGAAATG CAGTATGCTC CGCAGCCACC TCCAAGTGTG 
TGCCAGTTCA TAACCGCGAC GGAAATGACT CTCCAGCGCT ATGCAGCAGA TATAAATAGC 
AGACTTATCA GGCAGGTGGA GCGTCTGAAG CAGGAAGCAG TTACTGTACC TGTTTGTGAA 
GAGCAGCTGA AAGAAATCGA ACGCTGCATA AAAGTTTTCC TTCATGAAAA TGGAGAGGAG 
GGCTCACTGA GCTTAGCAAG TGTCATCATC TCTGCCCTTT GTACGCTGAC GAGGCGGAAT 
CTGATGATGG AAGGTGCAGC ATCCAGTGCT GGAGAGCAGC TGGTTGATCT GACTTCGAGA 
GATGGAGCAT GGTTTTTGGA GGAGCTCTGC AGCATGAGTG GGAATGTTAC TTGCCTTGTG 
CAGTTGCTGA AGCAGTGTCA TCTTGTACCC CAGGACTTAG ATATTCCTAA CCCAATTGAA 
GCATCAGAAG CTGTTCATTT AGCTAATGGA GTATACATCT CACTACAGGA ACTGAATTCC 
AACTTCCGGC AGATTATTTT CCCTGAAGCA CTTCGGTGCT TAATGAAAGG AGAATACACT 
TTAGAAAGTA TGCTCCATGA ATTAGATAAC CTTATTGAAC AAACAACTGA TGGAGTTTCT 
TTGCAAACAT TGGTTGAATC TCTTCAGGCC TACTTAAGAA ATGCAGCTAT GGGACTAGAG 
GAAGAAACAC ACACTCATTA CATTGATGTT GCAAGAGTAC TTCATGCTCA GTATGGCGAG 
CTGATTCAGC CAAGGAATGG TTCTGTGGAT GAAACTCCCA AAATGTCAGC TGGTCAGATG 
CTTTTGGTAG CCTTTGATGG AATGTTTGCT CAGGTGGAAA CTGCGTTTGG TTTATTGATT 
GAAAAGCTAA ACAAGATGGA AGTCCCTGTT GCTTGGCGTA AGATCGACAT CATTAGGGAA 
GCCCGCAGCA CTCAAGTTAA CTTCTTTGAT GATGACAATA ATCGCCAAGT TCTGGAGGAA 
ATCTTCTTTT TGAAGAGACT GCAAACAATT AAAGAATTTT TCAGACTCTG TGGTACCTTT 
TCTAAAACAT TGTCAGGAAT AAGTTCACTT GATGACCAGA ATGCAGTAAA CGGACCTGTT 
CAGATTGTCA ATGTGAAAGC CCTTTACAGA AACTCTTGCT TCAGTGAAGA CCAAATGGCC 
AAACCTATCA AGGCTTTTAC CGCTGACTTT GTGAGGCAGC TTTTAATTGG CCTTCCAAAT 
CAGGCTTTAG GACTGACTTT GTGCAGTTTC ATCAGCGCTT TGGGCGTGGA TATCATAGCT 
CAGGTAGAAG CCAAAGACTT TGGAGCAGAA AGCAAAGTTT CTGTGGATGA CCTGTGCAAG 
AAAGCTGTGG AGCACAATAT CCAGATTGGC AAGTTCTCAC AGCTGGTCAT GAACAGGGCA 
ACAGTGCTAG CTAGCTCGTA TGATACAGCG TGGAAGAAGC ATGATCTGGT GCGCAGACTC 
GAAACCAGTA TTTCTTCTTG CAAGACCAGT CTTCAGAGAG TGCAGCTGCA TATTGCCATG 
TTCCAATGGC AGCATGAAGA TCTCCTTATC AGTCGCCCAC AAGCCATTTC TGTTACTCCT 
CCACCTCGTT CAGCAATTTT AGCCAACATG AAGAAGAAAC TTCATACGCT CAGCCAGATT 
GAAGCTTCAA TTGCAACAGT TCAGGAGAAA CTAGCAGCAC TTGAAGCAAG CATTGAACAG 
CGTTTGAAAT GGGCAGGAGG AGCTAATCCT GCACTGGCAC CAGTCTTGCA AGATTTCGAT 
GCCACTATTG CTGAAAGAAG AAGCCTTGTC CTGAAAGAAA GTCAAAGAGC AAGTCAGGTT 
ACTTTCCTGT GCAGTAATAT CATCCACTTT GAAAGTTTAC GTACTAGAAC AGCAGAAGCC 
TTAAATCTTG ATGCTGCATT ATTTGAACTT CTCAAACGCT GTCAACAAAT GTGTTCATTT 
GCATCACAGT TCAACAGCTC AGTGTCTGAA TTGGAGCTTC GTCTGCTGCA GAGAGTGGGC 
ACTGGTCTTG AACATCCTAT TGGCAGCTCT GAGTGGCTTC ACTCAGCCCA CAAGCAGTTG 
ACACAGGAAA TTACTACACA GAGAACTGTA CAGGCAGAAA GAGAACAGCA AATAGAAACA 
GTTTGTGAAA CTATTCAAAA TCTGGTGGAT AATATTAAAA CTGTGCTCAC AGGTCATAAT 
AGACAACTTG GAGATGTCAA GCATCTACTG AAGGCTATGG CAAAGGATGA AGAAGCAGCT 
TTGGCAGATG GTGAAGATGT TCCCTATGAA AATAGTGTTA GGCAGTTCTT GGGCGAATAT 
AAATCATGGC AAGATAATAT TCAGACAGTT TTGTTTACAT TAGTTCAGGT TATGGGTCAA 
GTCCGCAATC AGGAACATGT TGAAATGCTG CAGGAAATAA CCCCCACCCT GAAAGAGCTA 
AAAACACAGA GCCAAAGTGT CTACAATAAT CTAGTGAGTT TTGCATCACC CTTAGTCACG 
GATACATCAA ATGAATGCTC AAGCCCAACA TCAGCTGCTA CATACCAGCC AACCTTTGCT 
GCAGCTGTAC GTAGCAATAC AGGGCAGAAA ACTCAGCCAG AGGTGATGTC ACAGAATGCA 
AGAAAGCTAA TTCAGAAAAA TCTTGCCACA TCAGCAGATA CTCCTCCAAG CACAGTCCCA 
GGAACTGGCA AAAGTGTTGC CTGTAGTCCC AAGAAGACAG CCAGGGACCC TAAAACAGGA 
AAAGCTGTGC AGGAAAGGAA TTCATATGCA GTTAGTGTGT GGAAACGAGT CAAAGCCAAG 
TTGGAGGGCC GAGATGTGGA TCCCAGCAGG AGAATGTCTG TTGCTGAACA GGTTGACTTT 
GTGATTAAGG AAGCAACTAA TCTAGATAAC TTGGCTCAGC TGTATGAAGG TTGGACTGCC 
TGGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RP11-345J4.5 ( XM_015294535.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150021.1 Gallus gallus Uncharacterized protein (RP11-345J4.5), transcript variant X2, mRNA.

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CloneID	OGa42448
Clone ID Related Accession (Same CDS sequence)	XM_015294535.1
Accession Version	XM_015294535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA ACCCAGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGAGTT AACAATGTGA CCAATCCTGA TTTTACCAGT 
GTTCCGCATG GGAATCGTGC TTTAGCCACA AAAGACATGA GGAAATCACA GGAGCGATCG 
TTGTCTTATT CTGACGAGTC TCGACTGTCA AATCTTCTTC GGAGGATTAC TCGGGAAGAC 
GACAGAGACC GAAGACTGGC TACTGTAAAG CAGCTGAAAG AATTCATTCA GCAACCAGAA 
AACAAACTGG TACTAGTTAA ACAACTGGAT AATATCCTGA CTGCCATTCA TGATGTACTC 
AATGAAAGTA GCAAATTGCT TCAAGAACTG AGACAGGAAG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTAAGCTA TGAGGCTGAG AAGATATTTA AATGGATTTT TAGCAAATTC 
AGCTTATCCA CAAAAGATGA AGTTAAACTT CTTTATTTGT GTGCAACCTA CAAAGCACTG 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTTTAATGC AGCTTGTAAT GACCAGCCTA 
CAGTCCATTC TAGAAAATGT GGATACACCA GAGTTACTGT GCAAATGTGT CAAGTGCATT 
CTTCTGTTGT CCCGATGTTA TCCGCACATT TTCAGCACCA ATTTTCGGGA CACAGTGGAC 
ATACTGGTTG GGTGGCATAT AGATCACACT CAGAAGCCTT CATTGACTCA GCAGGTGTCC 
GGGTGGCTGC AGAGCTTGGA GCCCTTTTGG GTGGCTGATC TCACTTTTTC TACCACACTC 
TTGGGTCAAT TTTTAGAAGA TATGGAAGCT TATGCTGAGG ACCTCAGCCA TGTGGCTTCT 
GGGGAGTCCC TAGATGAAGA TATTCCTCCT CCCTCTGTAT CACTACCTAA ACTGGCTGCG 
CTTCTTCGAG TTTTCAGTAC TGTGGTGAGA AGTATTGGGG AGCGCTTCAG CCCAATTAGA 
GGACCACCGA TTACAGAAGC ATATGTAACT GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACTGCAGCAA ACCAAGTATT CTTTTCAGAA GCAGTACTCA CAGCTGCCAA TGAGTGTGTT 
GGTGTTTTAC TTGGCAGTCT GGATCCAAGC ATGACCATTC ACTGTGACAT GGTTATCACC 
TACGGATTGG ATCAAATGGA AAATTGTCAG ACTTGTGGCA CTGATTATAT CATTTCAGTC 
CTGAATCTGT TGACGCTGAT TGTTGAACAA ATAAATACAA AACTACCATC GTCATTTGTA 
GAGAAATTAT TTATTCCAGA GTCTAAACTG CTTGTACTTC GTTATCATAA GGAAAAAGAG 
GTTGTTGCAG CAGCCCACGC TGTGTATCAG GCAGTACTGA GCCTGAAGAA CATTCCTGTT 
TTGGAGACTG CTTACAGGTT GATTCTAGGA GAAATGACCT GTGCTCTAAA CAGTCTTCTT 
TATAGTTTAC ATCTTCCCGA GGCCTGTTCT GAAATCCAGC ATGACTCTTT CAAGAAGCAT 
GTATTCACTG TGGAAAATGC AAAGTTTGTT GTTATATTTG ACCTCAGTGC CCTAAGTACT 
ATTGGAAATG CTAAGAACTC ATTAATTGGG ATGTGGGCCC TTTCGCCAAC TGTGTTTGCA 
CTACTGAGTA AAAATCTGAT GATTGTTCAT GGTGACATAG CAGTTCATTT TCCTGCTGTC 
CAGTATGCAG TACTCTATAC GTTGTATTCG CATTGTTCCA GACATGACCA TTTTATATCC 
AGTAGCCTCA GTTCTTCCTC TCCTTCCCTG TTCGATGGTG CAGTTATTAG TACTGTAACC 
ACAGCAACAA AAAAACATTT CTCAATCATA CTGAACCTTT TGGGGCTACT GTTGAAGAAG 
GATAACCTTA CTCAAGATAC CAGGAAACTG CTTATGACGT GGGCTTTGGA AGTGGCTGTT 
CTGATGAAGA AATCAGAGAC ATATGCACCC TTATTTTCTC TCCCATCTTT CCATAAGTTT 
TGCAAAGGAC TTCTAGCAAA CACCCTCCTG GAAGATGTGA ATATTTGTCT TCAAGCGTGT 
AGCAGCCTCC ATGCCCTGTC CTCCTCCCTA CCAGATGACC TTTTACAGAG ATGTGTTGAT 
GTGTGTCGTG TTCAACTAGT TCATAGCGGT GCTCGTGTAA GGCAGACTTT TGGAAAACTT 
CTGAAGTCAA TTCCCTTAGA TGTGGTGTTG AGCAACCGTA ACCACACAGA AATACAAGAA 
ATTTCTTTGG CTATAAGAAG TCATATGAGC AAAGCTCCAA GTAATACATT CCACCCACAG 
GATTTCTCTG ATGTCATTAG TTTTATTTTG TATGGAAGCA CTCATCGAAC TGGGAAAGAT 
AATTGGTTGG AAAGGTTATT CTATAGCTGT CAAAGGCTGG ATAAACGAGA TCAACCAACC 
ATCCCTCGTA ATCTGTTGAA GACTGATGCT GTTCTCTGGC AATGGGCTGT TTGGGAAGCA 
GCTCAGTTTA CTGTTCTTTC TAAGCTGAGA ACTCCTCTGG GTAGAGCCCA AGATACATTT 
CAAACAATTG AAGGTATTAT TAGGAGTCTT GCAGCGCATA CGCTAAACCC TGATCAAGAT 
GTTAGCCAGT GGACTACTGC AGACAATGAT GAGGGACACA GCAGCAATCA ACTTAGGCTT 
GTTCTCCTTC TACAGTATCT GGAGAACTTG GAAAAACTGA TGTACAATGC TTATGAAGGA 
TGTGCCAATG CCCTTACCTC TCCACCCAAG GTTATCAGGA CATTCTTTTA TACTAATCGC 
CAGACGTGCC AAGATTGGCT GACGCGGATT AGACTTTCCA TCATGAGAGT TGGACTGCTG 
GCCGGCCAGC CTGCTGTGAC AGTCAGACAT GGTTTTGACT TACTCACAGA AATGAAAACT 
AATAACAGTT TATCTCAGGG AAATGAATTG GAAGTGACAA TTATGATGCT GGTGGAAGCA 
TTATGTGAAC TTCACTGCCC TGAAGCTATT CAGGGTATTG CTGTCTGGTC TTCTGCTGTA 
GTCGGGAAGA GTCTTACCTG GATCAACTCT GTAGCTCAAC AGGCAGAAGG GCGGTTTGAA 
AAAGCAACTG CGGAGTACCA GGAGCACCTG TGTGCCATGA CAGGAGTAGA TTGCTGTATA 
TCGGGATTTG ACAAAACTGT TCTGAAGTTG GCCAATTCCA ACAGTGTGAA TAACGCCAGC 
CCAAAGCACT CCTTGAACGG AGAAACTAGA AAAACTGTTC TGACTAAGCC AAGTGACTGT 
TCACCTGAAG TTATAAATTA CTTGGGTAAC AAGGCATGTG AATGTTACAT TTCAATTGCT 
GATTGGTCTG CTGTTCAGGA ATGGCAAAAT ATGGTCCATG ACTTGAAGAA AAGCACGAGT 
AATACCTCAA TCAATCTGAA AGCAGATTTT AACTACATCA AATCCCTGAG CAGCTTTGAG 
TCTGGTGAAC TTACTGAATG CACTGAACAA CTGGAATTAT TACCAGGAGA AAATATCAAC 
CTACTTGCAG GGGGATCCAA AGAAAAAATA GACATGAAGA AGCTCCTTCC TAATATGCTA 
AGTCCTGACC CGAGAGAACT TCAAAAAGCA GTTGAAGTAC AACTTCTGAG AAGTTCGGTG 
TGTCTGGCAA CAGCTGTAAA CCACATGGAG CAGGAGCAAA AGTGGCAGTC AATATCTGAA 
AATGTTATAA AATACTTAAA GCAAACATCA CGAATAGCTA TTGGGCCTTT AAGACTTTCC 
ACGCTCACCG TGTCTCAGTC TTTACCAGTG TTGAGCACTC TTCAGTTGTA TTGTTCTTCT 
GCTTTAGAAA ACACCGTATC CAACAGGCTG ACATCAGAGG ACTGTCTTAT ACCTCTCTTC 
AATGATGCTC TGAGGTCATG TAAACAGCAT GATGTGAGGC CGTGGATGCA TGCACTGAGA 
TACACGGTGT ATCAGAATCA ACTGATGGGA AAGCTTAAAG AACCAACAGT CCCAATTAAG 
AGTCATCTAA TGGAGCTGGG CTTAACAGCA GCAAAATTTG CACGGAAGCG AGGAAATATT 
GCCCTGGCTA CACGGTTGCT TGCCCAGTGC AGTGAAGTTC AGCTAGGAAA AACCACCACT 
GCACAGGACT TAGTCCAGCA CTTTAAGAAA CTGTCTGCAC CAGGTCAGAT TGATGAAAAG 
TGGGGTCCTG AACTTGATAT TGAGAAAACA AAGCTGTTAT ACACAGCAGG TCAGTCAACA 
CATGCCATGG AAATGCTGAG TTCTTGTGCT ATATCATTTT GCAAATCTGC CAAGGCTGAA 
TATGCAGTTG CTAAATCTAT TCTTACGCTG GCTAAATGGA TTCAAGCAGA ATGGAAAGAG 
ATTTCAGGAC AGTTAAAACA AGTATATAGA GCTCGTCAAC AGCAGAATCA CTCTGGTCTG 
TCTACCTTGT CTAAGAACAT ATATACTTTG ATTGATCTCC CAGCTGTTAA TACGTTAGAA 
GAGGAATATC CCAGGATTGA AAGTGAATCT ACAGTAAATA TTGGAGTTGG AGAACCTGAC 
TTTATCTTGG GACAGCTGTA TCACTTGTCT TCAGTACAGG CACCTGAAGT GGCCAAGTCT 
TGGGCAGCCC TGGCTAGTTG GGCTTATAGA TGGGGCCGGA AAGTAGTTGA TAATGCCAGT 
CAGGGAGAAG GTGTTCGTCT GCTGCCCCGA GAGAAATCTG AGGTTCAGAG CTTGCTTCCA 
GACAATGTTA CAGAGGAAGA CAAAGAGAAG ATTTATGGGA TCCTTGGGCA AGCAGTGTGT 
CGTCCAGCTG GGATTCAAGA TGAAGATATG ACTCTGCAAA TCACTGAAAA TGAAGACAAT 
GAAGAAGATG ATATGGTGGA TGTTATATGG CGTCAGTTAT TAACAACTTG TCCCTGGCTT 
TCAGATCTTG ATGAAAATGC AACAGAAGGT TTAATTAAAG TATGGCGGAA AGTTGTAGAC 
AGAATCTTCA GTCTCTATAA ACTCTCCTGC AGTGCATATT TCACTTTCCT CAAACTCAAT 
GCTGGTCAGG TTCCACTTGA TGAGGATGAT CCAAGGCTGC ATTTAAGAAA TACTTCTGAG 
CAAAGCACTG ATGATGTTAT TGTGATGGCA ACCCTAAGAC TGTTAAGGCT GCTTGTTAAA 
CATGCTGGAG AGCTTAGACA GTATCTAGAG CATGGGCTAG AAACTACACC TACTGCTCCT 
TGGAGAGGTA TTATTCCTCA GCTCTTCTCT CGCCTCAATC ATCCAGAAGT GTATGTTCGT 
CAGAGTATTT GCAACTTACT GTGTCGAGTG GCTCAAGATT CTCCTCATCT CATACTGTAT 
CCTGCAATAG TGGGTACTAT TTCACTCAGC AGTGAATCCC AAACCTCAGG GAACAAGCTT 
CCTTCAGCGA TTCCCACTTT GCTAGGTAAT ATTCAAGGAG AAGAACTGTT GGGTGGTGAA 
TGTGATGGTG GGAGTCCACC AGCTTCTCAA GAAAGCACTA AAGATGACAC AAAAGTTGGA 
TTAAATGAAG ATCAAGCAAT GATGCAAGAT TGTTACAGCA AAATTGTGGA GAAACTCTCA 
ACTGCAAATC CAACAATGGT ATTGCAGGTT CAGATGCTAG TGGCTGAGCT GCGCAGAGTT 
ACAGTTCTGT GGGATGAACT TTGGTTGGGC GTTTTACTGC AGCAGCACAT GTATGTCCTC 
AGAAGGATTC AGCAACTGGA AGATGAAGTC AAAAGGGTCC AAAACAACAA CACTCTACGC 
AAAGAGGAGA AGATAGCAAT CATGCGTGAG AAGCATACAG CTCTAATGAA GCCCATTGTA 
TTTGCTTTGG AACATGTACG GAGCATCACT GCAGCTCCTA CAGAAACTCC TCATGAGAAA 
TGGTTTCAGG ATAACTATGG CGGTGCAATC GAAAATGCAC TAGAGAAACT TAAGAATCCT 
TTGAATCCTG CTAAGCCTGG AAGCAGCTGG CTCCCCTTTA AAGAGGTTAT GCTAAGTTTG 
CAACAGAGAG CACAGAAACG GGCTAGTTAT CTTTTACGGC TTGAAGAAAT CAGTCCTTGG 
TTGGCTGCCA TGATGAACAC TGAAATAGCG CTCCCTGGAG AAGTATCCAC CAGAGACACT 
GTCACAATTC ATAGTGTGGG CAGCACCATC ACAATTCTGC CTACCAAAAC CAAACCTAAA 
AAGCTGCTTT TCCTGGGCTC AGATGGGAAG AACTATCCAT ACTTGTTCAA AGGTTTAGAG 
GACTTGCACC TAGATGAAAG AATCATGCAA TTCCTATCAA TTGTGAACAC AATGTTTGCT 
ACCATTAACC GTCAAGAGAC ACCAAGGTTT CATGCACGGC ACTACTCTGT GACACCTCTG 
GGAACAAGAT CAGGCCTGAT CCAGTGGGTG GATGGAGCTA CACCTTTGTT TGGTCTTTAC 
AAACGATGGC AACAGCGGGA AGCTGCTTTA CAAGCACAAA AGGCTCAGGA TTCTTATCAG 
ACTCCTCAGA ATCCTGGAAT CGTGCCTAGA CCTAGTGAAC TGTACTACAG TAAAATTGGA 
CCTGCTTTAA AGGCAGTTGG GCTTAGTCTT GATGTGTCGC GTCGTGACTG GCCCTTGAAT 
GTGATGAGAG CTGTTCTAGA AGAACTGATG GAAGCAACTC CCCCAAATCT CCTAGCTAAG 
GAGCTCTGGT CGTCATGTAC TACACCAGAT GAATGGTGGA GAGTTACACA GTCTTATGCA 
AGATCCACTG CAGTTATGTC CATGGTTGGC TACATCATTG GTCTTGGAGA CAGACATCTG 
GATAATGTTC TTATAGATAT GACTACAGGA GAAGTTGTCC ACATAGATTA TAATGTTTGC 
TTTGAAAAAG GGAAGAGCCT TAGGGTACCA GAGAAGGTTC CATTCCGAAT GACACACAAT 
ATTGAAACAG CACTTGGAGT GACAGGAGTA GAGGGGGTTT TCAGGCTTTC TTGTGAACAG 
GTCCTTCATA TCATGCGGCG TGGGAGAGAA ACTTTGCTGA CGTTGCTTGA AGCTTTTGTT 
TATGATCCTC TGGTGGACTG GACAGCAGGG GGTGAGGCAG GATTTGCAGG AGCTGTGTAT 
GGTGGTGGCG GCCAGCAGGC TGAAAACAAG CAAAGCAAAA GAGAAATGGA AAGAGATATT 
ACACGTAGTC TCTTCTCTTC AAGAGTGGCT GAGATAAAGG TTAACTGGTT TAAGAACAGA 
GATGAAATGT TGGCTGTGCT TCCAAAGTTG GACAGTAGTT TAGAAGAATA TCTCAACCTG 
CAAGAACAGT TGACAGATGT AGAAAAATTG CAAGGAAAGC TACTGGAGGA GATAGAATTT 
CTTGAAGGTG CAGAAGGAGT GGATCATCCC TCTCACACAC TTCAGCACAG GTATTCTGAG 
CACACACAGT TGCAGACTCA ACAAAGAGCT GTCCAGGAGG CGATCCAGGT GAAGCTGAAT 
GAATTTGAGC AGTGGATAAC ACACTACCAA GCTGCTTTCA GTAATTTAGA GGCAACACAA 
CTTGCAAGTC TCCTTCAAGA AATTAGCACA CAAATGGACC TAGGTCCTCC GAGTTATGTC 
CCAGCAACAG CATTTCTGCA AAATGCAGGC CAGGCACATC TCATCAGTCA GTGTGAACAA 
TTAGAAGGGG AGGTTGGTGC TCTTCTCCAG CAGAGAAGAT CTGTTTTACG TGGTTGCCTC 
GAACAGCTGC ACAACTATGC AACAGTGGCT CTTCAGTATC CGAAAGCTGT GTTTCAGAAG 
CACCGAATAG AGCAGTGGAA AACTTGGATG GAAGAACTCA TCTGCAACAT GACAATAGAG 
CGCTGTCAGG ATATCTTTAG GAAGTATGAA ATGCAGTATG CTCCGCAGCC ACCTCCAAGT 
GTGTGCCAGT TCATAACCGC GACGGAAATG ACTCTCCAGC GCTATGCAGC AGATATAAAT 
AGCAGACTTA TCAGGCAGGT GGAGCGTCTG AAGCAGGAAG CAGTTACTGT ACCTGTTTGT 
GAAGAGCAGC TGAAAGAAAT CGAACGCTGC ATAAAAGTTT TCCTTCATGA AAATGGAGAG 
GAGGGCTCAC TGAGCTTAGC AAGTGTCATC ATCTCTGCCC TTTGTACGCT GACGAGGCGG 
AATCTGATGA TGGAAGGTGC AGCATCCAGT GCTGGAGAGC AGCTGGTTGA TCTGACTTCG 
AGAGATGGAG CATGGTTTTT GGAGGAGCTC TGCAGCATGA GTGGGAATGT TACTTGCCTT 
GTGCAGTTGC TGAAGCAGTG TCATCTTGTA CCCCAGGACT TAGATATTCC TAACCCAATT 
GAAGCATCAG AAGCTGTTCA TTTAGCTAAT GGAGTATACA TCTCACTACA GGAACTGAAT 
TCCAACTTCC GGCAGATTAT TTTCCCTGAA GCACTTCGGT GCTTAATGAA AGGAGAATAC 
ACTTTAGAAA GTATGCTCCA TGAATTAGAT AACCTTATTG AACAAACAAC TGATGGAGTT 
TCTTTGCAAA CATTGGTTGA ATCTCTTCAG GCCTACTTAA GAAATGCAGC TATGGGACTA 
GAGGAAGAAA CACACACTCA TTACATTGAT GTTGCAAGAG TACTTCATGC TCAGTATGGC 
GAGCTGATTC AGCCAAGGAA TGGTTCTGTG GATGAAACTC CCAAAATGTC AGCTGGTCAG 
ATGCTTTTGG TAGCCTTTGA TGGAATGTTT GCTCAGGTGG AAACTGCGTT TGGTTTATTG 
ATTGAAAAGC TAAACAAGAT GGAAGTCCCT GTTGCTTGGC GTAAGATCGA CATCATTAGG 
GAAGCCCGCA GCACTCAAGT TAACTTCTTT GATGATGACA ATAATCGCCA AGTTCTGGAG 
GAAATCTTCT TTTTGAAGAG ACTGCAAACA ATTAAAGAAT TTTTCAGACT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG AATAAGTTCA CTTGATGACC AGAATGCAGT AAACGGACCT 
GTTCAGATTG TCAATGTGAA AGCCCTTTAC AGAAACTCTT GCTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCTTT TACCGCTGAC TTTGTGAGGC AGCTTTTAAT TGGCCTTCCA 
AATCAGGCTT TAGGACTGAC TTTGTGCAGT TTCATCAGCG CTTTGGGCGT GGATATCATA 
GCTCAGGTAG AAGCCAAAGA CTTTGGAGCA GAAAGCAAAG TTTCTGTGGA TGACCTGTGC 
AAGAAAGCTG TGGAGCACAA TATCCAGATT GGCAAGTTCT CACAGCTGGT CATGAACAGG 
GCAACAGTGC TAGCTAGCTC GTATGATACA GCGTGGAAGA AGCATGATCT GGTGCGCAGA 
CTCGAAACCA GTATTTCTTC TTGCAAGACC AGTCTTCAGA GAGTGCAGCT GCATATTGCC 
ATGTTCCAAT GGCAGCATGA AGATCTCCTT ATCAGTCGCC CACAAGCCAT TTCTGTTACT 
CCTCCACCTC GTTCAGCAAT TTTAGCCAAC ATGAAGAAGA AACTTCATAC GCTCAGCCAG 
ATTGAAGCTT CAATTGCAAC AGTTCAGGAG AAACTAGCAG CACTTGAAGC AAGCATTGAA 
CAGCGTTTGA AATGGGCAGG AGGAGCTAAT CCTGCACTGG CACCAGTCTT GCAAGATTTC 
GATGCCACTA TTGCTGAAAG AAGAAGCCTT GTCCTGAAAG AAAGTCAAAG AGCAAGTCAG 
GTTACTTTCC TGTGCAGTAA TATCATCCAC TTTGAAAGTT TACGTACTAG AACAGCAGAA 
GCCTTAAATC TTGATGCTGC ATTATTTGAA CTTCTCAAAC GCTGTCAACA AATGTGTTCA 
TTTGCATCAC AGTTCAACAG CTCAGTGTCT GAATTGGAGC TTCGTCTGCT GCAGAGAGTG 
GGCACTGGTC TTGAACATCC TATTGGCAGC TCTGAGTGGC TTCACTCAGC CCACAAGCAG 
TTGACACAGG AAATTACTAC ACAGAGAACT GTACAGGCAG AAAGAGAACA GCAAATAGAA 
ACAGTTTGTG AAACTATTCA AAATCTGGTG GATAATATTA AAACTGTGCT CACAGGTCAT 
AATAGACAAC TTGGAGATGT CAAGCATCTA CTGAAGGCTA TGGCAAAGGA TGAAGAAGCA 
GCTTTGGCAG ATGGTGAAGA TGTTCCCTAT GAAAATAGTG TTAGGCAGTT CTTGGGCGAA 
TATAAATCAT GGCAAGATAA TATTCAGACA GTTTTGTTTA CATTAGTTCA GGTTATGGGT 
CAAGTCCGCA ATCAGGAACA TGTTGAAATG CTGCAGGAAA TAACCCCCAC CCTGAAAGAG 
CTAAAAACAC AGAGCCAAAG TGTCTACAAT AATCTAGTGA GTTTTGCATC ACCCTTAGTC 
ACGGATACAT CAAATGAATG CTCAAGCCCA ACATCAGCTG CTACATACCA GCCAACCTTT 
GCTGCAGCTG TACGTAGCAA TACAGGGCAG AAAACTCAGC CAGAGGTGAT GTCACAGAAT 
GCAAGAAAGC TAATTCAGAA AAATCTTGCC ACATCAGCAG ATACTCCTCC AAGCACAGTC 
CCAGGAACTG GCAAAAGTGT TGCCTGTAGT CCCAAGAAGA CAGCCAGGGA CCCTAAAACA 
GGAAAAGCTG TGCAGGAAAG GAATTCATAT GCAGTTAGTG TGTGGAAACG AGTCAAAGCC 
AAGTTGGAGG GCCGAGATGT GGATCCCAGC AGGAGAATGT CTGTTGCTGA ACAGGTTGAC 
TTTGTGATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACT 
GCCTGGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150021.1
CDS	103..10854
Translation	MTGNPGGYSVNGGSGENTYGRKSLGQELRVNNVTNPDFTSVPHGNRALATKDMRKSQERSLSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILTAIHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSLSTKDEVKLLYLCATYKALETVGEKKAFSSLMQLVMTSLQSILENVDTPELLCKCVKCILLLSRCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLTFSTTLLGQFLEDMEAYAEDLSHVASGESLDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQMENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPESKLLVLRYHKEKEVVAAAHAVYQAVLSLKNIPVLETAYRLILGEMTCALNSLLYSLHLPEACSEIQHDSFKKHVFTVENAKFVVIFDLSALSTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDIAVHFPAVQYAVLYTLYSHCSRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGLLLKKDNLTQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLLEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGARVRQTFGKLLKSIPLDVVLSNRNHTEIQEISLAIRSHMSKAPSNTFHPQDFSDVISFILYGSTHRTGKDNWLERLFYSCQRLDKRDQPTIPRNLLKTDAVLWQWAVWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNNSLSQGNELEVTIMMLVEALCELHCPEAIQGIAVWSSAVVGKSLTWINSVAQQAEGRFEKATAEYQEHLCAMTGVDCCISGFDKTVLKLANSNSVNNASPKHSLNGETRKTVLTKPSDCSPEVINYLGNKACECYISIADWSAVQEWQNMVHDLKKSTSNTSINLKADFNYIKSLSSFESGELTECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKAVEVQLLRSSVCLATAVNHMEQEQKWQSISENVIKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLTSEDCLIPLFNDALRSCKQHDVRPWMHALRYTVYQNQLMGKLKEPTVPIKSHLMELGLTAAKFARKRGNIALATRLLAQCSEVQLGKTTTAQDLVQHFKKLSAPGQIDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRARQQQNHSGLSTLSKNIYTLIDLPAVNTLEEEYPRIESESTVNIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQSLLPDNVTEEDKEKIYGILGQAVCRPAGIQDEDMTLQITENEDNEEDDMVDVIWRQLLTTCPWLSDLDENATEGLIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVPLDEDDPRLHLRNTSEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQTSGNKLPSAIPTLLGNIQGEELLGGECDGGSPPASQESTKDDTKVGLNEDQAMMQDCYSKIVEKLSTANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPTETPHEKWFQDNYGGAIENALEKLKNPLNPAKPGSSWLPFKEVMLSLQQRAQKRASYLLRLEEISPWLAAMMNTEIALPGEVSTRDTVTIHSVGSTITILPTKTKPKKLLFLGSDGKNYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLNVMRAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLAVLPKLDSSLEEYLNLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFSNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHNYATVALQYPKAVFQKHRIEQWKTWMEELICNMTIERCQDIFRKYEMQYAPQPPPSVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEEQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPIEASEAVHLANGVYISLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDNLIEQTTDGVSLQTLVESLQAYLRNAAMGLEEETHTHYIDVARVLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLIEKLNKMEVPVAWRKIDIIREARSTQVNFFDDDNNRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGISSLDDQNAVNGPVQIVNVKALYRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLISRPQAISVTPPPRSAILANMKKKLHTLSQIEASIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFDATIAERRSLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELLKRCQQMCSFASQFNSSVSELELRLLQRVGTGLEHPIGSSEWLHSAHKQLTQEITTQRTVQAEREQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQVMGQVRNQEHVEMLQEITPTLKELKTQSQSVYNNLVSFASPLVTDTSNECSSPTSAATYQPTFAAAVRSNTGQKTQPEVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKTARDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPSRRMSVAEQVDFVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_015294535.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Uncharacterized protein (RP11-345J4.5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294535.1

ORF Insert Sequence:

ATGACTGGCA ACCCAGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGAGTT AACAATGTGA CCAATCCTGA TTTTACCAGT 
GTTCCGCATG GGAATCGTGC TTTAGCCACA AAAGACATGA GGAAATCACA GGAGCGATCG 
TTGTCTTATT CTGACGAGTC TCGACTGTCA AATCTTCTTC GGAGGATTAC TCGGGAAGAC 
GACAGAGACC GAAGACTGGC TACTGTAAAG CAGCTGAAAG AATTCATTCA GCAACCAGAA 
AACAAACTGG TACTAGTTAA ACAACTGGAT AATATCCTGA CTGCCATTCA TGATGTACTC 
AATGAAAGTA GCAAATTGCT TCAAGAACTG AGACAGGAAG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTAAGCTA TGAGGCTGAG AAGATATTTA AATGGATTTT TAGCAAATTC 
AGCTTATCCA CAAAAGATGA AGTTAAACTT CTTTATTTGT GTGCAACCTA CAAAGCACTG 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTTTAATGC AGCTTGTAAT GACCAGCCTA 
CAGTCCATTC TAGAAAATGT GGATACACCA GAGTTACTGT GCAAATGTGT CAAGTGCATT 
CTTCTGTTGT CCCGATGTTA TCCGCACATT TTCAGCACCA ATTTTCGGGA CACAGTGGAC 
ATACTGGTTG GGTGGCATAT AGATCACACT CAGAAGCCTT CATTGACTCA GCAGGTGTCC 
GGGTGGCTGC AGAGCTTGGA GCCCTTTTGG GTGGCTGATC TCACTTTTTC TACCACACTC 
TTGGGTCAAT TTTTAGAAGA TATGGAAGCT TATGCTGAGG ACCTCAGCCA TGTGGCTTCT 
GGGGAGTCCC TAGATGAAGA TATTCCTCCT CCCTCTGTAT CACTACCTAA ACTGGCTGCG 
CTTCTTCGAG TTTTCAGTAC TGTGGTGAGA AGTATTGGGG AGCGCTTCAG CCCAATTAGA 
GGACCACCGA TTACAGAAGC ATATGTAACT GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACTGCAGCAA ACCAAGTATT CTTTTCAGAA GCAGTACTCA CAGCTGCCAA TGAGTGTGTT 
GGTGTTTTAC TTGGCAGTCT GGATCCAAGC ATGACCATTC ACTGTGACAT GGTTATCACC 
TACGGATTGG ATCAAATGGA AAATTGTCAG ACTTGTGGCA CTGATTATAT CATTTCAGTC 
CTGAATCTGT TGACGCTGAT TGTTGAACAA ATAAATACAA AACTACCATC GTCATTTGTA 
GAGAAATTAT TTATTCCAGA GTCTAAACTG CTTGTACTTC GTTATCATAA GGAAAAAGAG 
GTTGTTGCAG CAGCCCACGC TGTGTATCAG GCAGTACTGA GCCTGAAGAA CATTCCTGTT 
TTGGAGACTG CTTACAGGTT GATTCTAGGA GAAATGACCT GTGCTCTAAA CAGTCTTCTT 
TATAGTTTAC ATCTTCCCGA GGCCTGTTCT GAAATCCAGC ATGACTCTTT CAAGAAGCAT 
GTATTCACTG TGGAAAATGC AAAGTTTGTT GTTATATTTG ACCTCAGTGC CCTAAGTACT 
ATTGGAAATG CTAAGAACTC ATTAATTGGG ATGTGGGCCC TTTCGCCAAC TGTGTTTGCA 
CTACTGAGTA AAAATCTGAT GATTGTTCAT GGTGACATAG CAGTTCATTT TCCTGCTGTC 
CAGTATGCAG TACTCTATAC GTTGTATTCG CATTGTTCCA GACATGACCA TTTTATATCC 
AGTAGCCTCA GTTCTTCCTC TCCTTCCCTG TTCGATGGTG CAGTTATTAG TACTGTAACC 
ACAGCAACAA AAAAACATTT CTCAATCATA CTGAACCTTT TGGGGCTACT GTTGAAGAAG 
GATAACCTTA CTCAAGATAC CAGGAAACTG CTTATGACGT GGGCTTTGGA AGTGGCTGTT 
CTGATGAAGA AATCAGAGAC ATATGCACCC TTATTTTCTC TCCCATCTTT CCATAAGTTT 
TGCAAAGGAC TTCTAGCAAA CACCCTCCTG GAAGATGTGA ATATTTGTCT TCAAGCGTGT 
AGCAGCCTCC ATGCCCTGTC CTCCTCCCTA CCAGATGACC TTTTACAGAG ATGTGTTGAT 
GTGTGTCGTG TTCAACTAGT TCATAGCGGT GCTCGTGTAA GGCAGACTTT TGGAAAACTT 
CTGAAGTCAA TTCCCTTAGA TGTGGTGTTG AGCAACCGTA ACCACACAGA AATACAAGAA 
ATTTCTTTGG CTATAAGAAG TCATATGAGC AAAGCTCCAA GTAATACATT CCACCCACAG 
GATTTCTCTG ATGTCATTAG TTTTATTTTG TATGGAAGCA CTCATCGAAC TGGGAAAGAT 
AATTGGTTGG AAAGGTTATT CTATAGCTGT CAAAGGCTGG ATAAACGAGA TCAACCAACC 
ATCCCTCGTA ATCTGTTGAA GACTGATGCT GTTCTCTGGC AATGGGCTGT TTGGGAAGCA 
GCTCAGTTTA CTGTTCTTTC TAAGCTGAGA ACTCCTCTGG GTAGAGCCCA AGATACATTT 
CAAACAATTG AAGGTATTAT TAGGAGTCTT GCAGCGCATA CGCTAAACCC TGATCAAGAT 
GTTAGCCAGT GGACTACTGC AGACAATGAT GAGGGACACA GCAGCAATCA ACTTAGGCTT 
GTTCTCCTTC TACAGTATCT GGAGAACTTG GAAAAACTGA TGTACAATGC TTATGAAGGA 
TGTGCCAATG CCCTTACCTC TCCACCCAAG GTTATCAGGA CATTCTTTTA TACTAATCGC 
CAGACGTGCC AAGATTGGCT GACGCGGATT AGACTTTCCA TCATGAGAGT TGGACTGCTG 
GCCGGCCAGC CTGCTGTGAC AGTCAGACAT GGTTTTGACT TACTCACAGA AATGAAAACT 
AATAACAGTT TATCTCAGGG AAATGAATTG GAAGTGACAA TTATGATGCT GGTGGAAGCA 
TTATGTGAAC TTCACTGCCC TGAAGCTATT CAGGGTATTG CTGTCTGGTC TTCTGCTGTA 
GTCGGGAAGA GTCTTACCTG GATCAACTCT GTAGCTCAAC AGGCAGAAGG GCGGTTTGAA 
AAAGCAACTG CGGAGTACCA GGAGCACCTG TGTGCCATGA CAGGAGTAGA TTGCTGTATA 
TCGGGATTTG ACAAAACTGT TCTGAAGTTG GCCAATTCCA ACAGTGTGAA TAACGCCAGC 
CCAAAGCACT CCTTGAACGG AGAAACTAGA AAAACTGTTC TGACTAAGCC AAGTGACTGT 
TCACCTGAAG TTATAAATTA CTTGGGTAAC AAGGCATGTG AATGTTACAT TTCAATTGCT 
GATTGGTCTG CTGTTCAGGA ATGGCAAAAT ATGGTCCATG ACTTGAAGAA AAGCACGAGT 
AATACCTCAA TCAATCTGAA AGCAGATTTT AACTACATCA AATCCCTGAG CAGCTTTGAG 
TCTGGTGAAC TTACTGAATG CACTGAACAA CTGGAATTAT TACCAGGAGA AAATATCAAC 
CTACTTGCAG GGGGATCCAA AGAAAAAATA GACATGAAGA AGCTCCTTCC TAATATGCTA 
AGTCCTGACC CGAGAGAACT TCAAAAAGCA GTTGAAGTAC AACTTCTGAG AAGTTCGGTG 
TGTCTGGCAA CAGCTGTAAA CCACATGGAG CAGGAGCAAA AGTGGCAGTC AATATCTGAA 
AATGTTATAA AATACTTAAA GCAAACATCA CGAATAGCTA TTGGGCCTTT AAGACTTTCC 
ACGCTCACCG TGTCTCAGTC TTTACCAGTG TTGAGCACTC TTCAGTTGTA TTGTTCTTCT 
GCTTTAGAAA ACACCGTATC CAACAGGCTG ACATCAGAGG ACTGTCTTAT ACCTCTCTTC 
AATGATGCTC TGAGGTCATG TAAACAGCAT GATGTGAGGC CGTGGATGCA TGCACTGAGA 
TACACGGTGT ATCAGAATCA ACTGATGGGA AAGCTTAAAG AACCAACAGT CCCAATTAAG 
AGTCATCTAA TGGAGCTGGG CTTAACAGCA GCAAAATTTG CACGGAAGCG AGGAAATATT 
GCCCTGGCTA CACGGTTGCT TGCCCAGTGC AGTGAAGTTC AGCTAGGAAA AACCACCACT 
GCACAGGACT TAGTCCAGCA CTTTAAGAAA CTGTCTGCAC CAGGTCAGAT TGATGAAAAG 
TGGGGTCCTG AACTTGATAT TGAGAAAACA AAGCTGTTAT ACACAGCAGG TCAGTCAACA 
CATGCCATGG AAATGCTGAG TTCTTGTGCT ATATCATTTT GCAAATCTGC CAAGGCTGAA 
TATGCAGTTG CTAAATCTAT TCTTACGCTG GCTAAATGGA TTCAAGCAGA ATGGAAAGAG 
ATTTCAGGAC AGTTAAAACA AGTATATAGA GCTCGTCAAC AGCAGAATCA CTCTGGTCTG 
TCTACCTTGT CTAAGAACAT ATATACTTTG ATTGATCTCC CAGCTGTTAA TACGTTAGAA 
GAGGAATATC CCAGGATTGA AAGTGAATCT ACAGTAAATA TTGGAGTTGG AGAACCTGAC 
TTTATCTTGG GACAGCTGTA TCACTTGTCT TCAGTACAGG CACCTGAAGT GGCCAAGTCT 
TGGGCAGCCC TGGCTAGTTG GGCTTATAGA TGGGGCCGGA AAGTAGTTGA TAATGCCAGT 
CAGGGAGAAG GTGTTCGTCT GCTGCCCCGA GAGAAATCTG AGGTTCAGAG CTTGCTTCCA 
GACAATGTTA CAGAGGAAGA CAAAGAGAAG ATTTATGGGA TCCTTGGGCA AGCAGTGTGT 
CGTCCAGCTG GGATTCAAGA TGAAGATATG ACTCTGCAAA TCACTGAAAA TGAAGACAAT 
GAAGAAGATG ATATGGTGGA TGTTATATGG CGTCAGTTAT TAACAACTTG TCCCTGGCTT 
TCAGATCTTG ATGAAAATGC AACAGAAGGT TTAATTAAAG TATGGCGGAA AGTTGTAGAC 
AGAATCTTCA GTCTCTATAA ACTCTCCTGC AGTGCATATT TCACTTTCCT CAAACTCAAT 
GCTGGTCAGG TTCCACTTGA TGAGGATGAT CCAAGGCTGC ATTTAAGAAA TACTTCTGAG 
CAAAGCACTG ATGATGTTAT TGTGATGGCA ACCCTAAGAC TGTTAAGGCT GCTTGTTAAA 
CATGCTGGAG AGCTTAGACA GTATCTAGAG CATGGGCTAG AAACTACACC TACTGCTCCT 
TGGAGAGGTA TTATTCCTCA GCTCTTCTCT CGCCTCAATC ATCCAGAAGT GTATGTTCGT 
CAGAGTATTT GCAACTTACT GTGTCGAGTG GCTCAAGATT CTCCTCATCT CATACTGTAT 
CCTGCAATAG TGGGTACTAT TTCACTCAGC AGTGAATCCC AAACCTCAGG GAACAAGCTT 
CCTTCAGCGA TTCCCACTTT GCTAGGTAAT ATTCAAGGAG AAGAACTGTT GGGTGGTGAA 
TGTGATGGTG GGAGTCCACC AGCTTCTCAA GAAAGCACTA AAGATGACAC AAAAGTTGGA 
TTAAATGAAG ATCAAGCAAT GATGCAAGAT TGTTACAGCA AAATTGTGGA GAAACTCTCA 
ACTGCAAATC CAACAATGGT ATTGCAGGTT CAGATGCTAG TGGCTGAGCT GCGCAGAGTT 
ACAGTTCTGT GGGATGAACT TTGGTTGGGC GTTTTACTGC AGCAGCACAT GTATGTCCTC 
AGAAGGATTC AGCAACTGGA AGATGAAGTC AAAAGGGTCC AAAACAACAA CACTCTACGC 
AAAGAGGAGA AGATAGCAAT CATGCGTGAG AAGCATACAG CTCTAATGAA GCCCATTGTA 
TTTGCTTTGG AACATGTACG GAGCATCACT GCAGCTCCTA CAGAAACTCC TCATGAGAAA 
TGGTTTCAGG ATAACTATGG CGGTGCAATC GAAAATGCAC TAGAGAAACT TAAGAATCCT 
TTGAATCCTG CTAAGCCTGG AAGCAGCTGG CTCCCCTTTA AAGAGGTTAT GCTAAGTTTG 
CAACAGAGAG CACAGAAACG GGCTAGTTAT CTTTTACGGC TTGAAGAAAT CAGTCCTTGG 
TTGGCTGCCA TGATGAACAC TGAAATAGCG CTCCCTGGAG AAGTATCCAC CAGAGACACT 
GTCACAATTC ATAGTGTGGG CAGCACCATC ACAATTCTGC CTACCAAAAC CAAACCTAAA 
AAGCTGCTTT TCCTGGGCTC AGATGGGAAG AACTATCCAT ACTTGTTCAA AGGTTTAGAG 
GACTTGCACC TAGATGAAAG AATCATGCAA TTCCTATCAA TTGTGAACAC AATGTTTGCT 
ACCATTAACC GTCAAGAGAC ACCAAGGTTT CATGCACGGC ACTACTCTGT GACACCTCTG 
GGAACAAGAT CAGGCCTGAT CCAGTGGGTG GATGGAGCTA CACCTTTGTT TGGTCTTTAC 
AAACGATGGC AACAGCGGGA AGCTGCTTTA CAAGCACAAA AGGCTCAGGA TTCTTATCAG 
ACTCCTCAGA ATCCTGGAAT CGTGCCTAGA CCTAGTGAAC TGTACTACAG TAAAATTGGA 
CCTGCTTTAA AGGCAGTTGG GCTTAGTCTT GATGTGTCGC GTCGTGACTG GCCCTTGAAT 
GTGATGAGAG CTGTTCTAGA AGAACTGATG GAAGCAACTC CCCCAAATCT CCTAGCTAAG 
GAGCTCTGGT CGTCATGTAC TACACCAGAT GAATGGTGGA GAGTTACACA GTCTTATGCA 
AGATCCACTG CAGTTATGTC CATGGTTGGC TACATCATTG GTCTTGGAGA CAGACATCTG 
GATAATGTTC TTATAGATAT GACTACAGGA GAAGTTGTCC ACATAGATTA TAATGTTTGC 
TTTGAAAAAG GGAAGAGCCT TAGGGTACCA GAGAAGGTTC CATTCCGAAT GACACACAAT 
ATTGAAACAG CACTTGGAGT GACAGGAGTA GAGGGGGTTT TCAGGCTTTC TTGTGAACAG 
GTCCTTCATA TCATGCGGCG TGGGAGAGAA ACTTTGCTGA CGTTGCTTGA AGCTTTTGTT 
TATGATCCTC TGGTGGACTG GACAGCAGGG GGTGAGGCAG GATTTGCAGG AGCTGTGTAT 
GGTGGTGGCG GCCAGCAGGC TGAAAACAAG CAAAGCAAAA GAGAAATGGA AAGAGATATT 
ACACGTAGTC TCTTCTCTTC AAGAGTGGCT GAGATAAAGG TTAACTGGTT TAAGAACAGA 
GATGAAATGT TGGCTGTGCT TCCAAAGTTG GACAGTAGTT TAGAAGAATA TCTCAACCTG 
CAAGAACAGT TGACAGATGT AGAAAAATTG CAAGGAAAGC TACTGGAGGA GATAGAATTT 
CTTGAAGGTG CAGAAGGAGT GGATCATCCC TCTCACACAC TTCAGCACAG GTATTCTGAG 
CACACACAGT TGCAGACTCA ACAAAGAGCT GTCCAGGAGG CGATCCAGGT GAAGCTGAAT 
GAATTTGAGC AGTGGATAAC ACACTACCAA GCTGCTTTCA GTAATTTAGA GGCAACACAA 
CTTGCAAGTC TCCTTCAAGA AATTAGCACA CAAATGGACC TAGGTCCTCC GAGTTATGTC 
CCAGCAACAG CATTTCTGCA AAATGCAGGC CAGGCACATC TCATCAGTCA GTGTGAACAA 
TTAGAAGGGG AGGTTGGTGC TCTTCTCCAG CAGAGAAGAT CTGTTTTACG TGGTTGCCTC 
GAACAGCTGC ACAACTATGC AACAGTGGCT CTTCAGTATC CGAAAGCTGT GTTTCAGAAG 
CACCGAATAG AGCAGTGGAA AACTTGGATG GAAGAACTCA TCTGCAACAT GACAATAGAG 
CGCTGTCAGG ATATCTTTAG GAAGTATGAA ATGCAGTATG CTCCGCAGCC ACCTCCAAGT 
GTGTGCCAGT TCATAACCGC GACGGAAATG ACTCTCCAGC GCTATGCAGC AGATATAAAT 
AGCAGACTTA TCAGGCAGGT GGAGCGTCTG AAGCAGGAAG CAGTTACTGT ACCTGTTTGT 
GAAGAGCAGC TGAAAGAAAT CGAACGCTGC ATAAAAGTTT TCCTTCATGA AAATGGAGAG 
GAGGGCTCAC TGAGCTTAGC AAGTGTCATC ATCTCTGCCC TTTGTACGCT GACGAGGCGG 
AATCTGATGA TGGAAGGTGC AGCATCCAGT GCTGGAGAGC AGCTGGTTGA TCTGACTTCG 
AGAGATGGAG CATGGTTTTT GGAGGAGCTC TGCAGCATGA GTGGGAATGT TACTTGCCTT 
GTGCAGTTGC TGAAGCAGTG TCATCTTGTA CCCCAGGACT TAGATATTCC TAACCCAATT 
GAAGCATCAG AAGCTGTTCA TTTAGCTAAT GGAGTATACA TCTCACTACA GGAACTGAAT 
TCCAACTTCC GGCAGATTAT TTTCCCTGAA GCACTTCGGT GCTTAATGAA AGGAGAATAC 
ACTTTAGAAA GTATGCTCCA TGAATTAGAT AACCTTATTG AACAAACAAC TGATGGAGTT 
TCTTTGCAAA CATTGGTTGA ATCTCTTCAG GCCTACTTAA GAAATGCAGC TATGGGACTA 
GAGGAAGAAA CACACACTCA TTACATTGAT GTTGCAAGAG TACTTCATGC TCAGTATGGC 
GAGCTGATTC AGCCAAGGAA TGGTTCTGTG GATGAAACTC CCAAAATGTC AGCTGGTCAG 
ATGCTTTTGG TAGCCTTTGA TGGAATGTTT GCTCAGGTGG AAACTGCGTT TGGTTTATTG 
ATTGAAAAGC TAAACAAGAT GGAAGTCCCT GTTGCTTGGC GTAAGATCGA CATCATTAGG 
GAAGCCCGCA GCACTCAAGT TAACTTCTTT GATGATGACA ATAATCGCCA AGTTCTGGAG 
GAAATCTTCT TTTTGAAGAG ACTGCAAACA ATTAAAGAAT TTTTCAGACT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG AATAAGTTCA CTTGATGACC AGAATGCAGT AAACGGACCT 
GTTCAGATTG TCAATGTGAA AGCCCTTTAC AGAAACTCTT GCTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCTTT TACCGCTGAC TTTGTGAGGC AGCTTTTAAT TGGCCTTCCA 
AATCAGGCTT TAGGACTGAC TTTGTGCAGT TTCATCAGCG CTTTGGGCGT GGATATCATA 
GCTCAGGTAG AAGCCAAAGA CTTTGGAGCA GAAAGCAAAG TTTCTGTGGA TGACCTGTGC 
AAGAAAGCTG TGGAGCACAA TATCCAGATT GGCAAGTTCT CACAGCTGGT CATGAACAGG 
GCAACAGTGC TAGCTAGCTC GTATGATACA GCGTGGAAGA AGCATGATCT GGTGCGCAGA 
CTCGAAACCA GTATTTCTTC TTGCAAGACC AGTCTTCAGA GAGTGCAGCT GCATATTGCC 
ATGTTCCAAT GGCAGCATGA AGATCTCCTT ATCAGTCGCC CACAAGCCAT TTCTGTTACT 
CCTCCACCTC GTTCAGCAAT TTTAGCCAAC ATGAAGAAGA AACTTCATAC GCTCAGCCAG 
ATTGAAGCTT CAATTGCAAC AGTTCAGGAG AAACTAGCAG CACTTGAAGC AAGCATTGAA 
CAGCGTTTGA AATGGGCAGG AGGAGCTAAT CCTGCACTGG CACCAGTCTT GCAAGATTTC 
GATGCCACTA TTGCTGAAAG AAGAAGCCTT GTCCTGAAAG AAAGTCAAAG AGCAAGTCAG 
GTTACTTTCC TGTGCAGTAA TATCATCCAC TTTGAAAGTT TACGTACTAG AACAGCAGAA 
GCCTTAAATC TTGATGCTGC ATTATTTGAA CTTCTCAAAC GCTGTCAACA AATGTGTTCA 
TTTGCATCAC AGTTCAACAG CTCAGTGTCT GAATTGGAGC TTCGTCTGCT GCAGAGAGTG 
GGCACTGGTC TTGAACATCC TATTGGCAGC TCTGAGTGGC TTCACTCAGC CCACAAGCAG 
TTGACACAGG AAATTACTAC ACAGAGAACT GTACAGGCAG AAAGAGAACA GCAAATAGAA 
ACAGTTTGTG AAACTATTCA AAATCTGGTG GATAATATTA AAACTGTGCT CACAGGTCAT 
AATAGACAAC TTGGAGATGT CAAGCATCTA CTGAAGGCTA TGGCAAAGGA TGAAGAAGCA 
GCTTTGGCAG ATGGTGAAGA TGTTCCCTAT GAAAATAGTG TTAGGCAGTT CTTGGGCGAA 
TATAAATCAT GGCAAGATAA TATTCAGACA GTTTTGTTTA CATTAGTTCA GGTTATGGGT 
CAAGTCCGCA ATCAGGAACA TGTTGAAATG CTGCAGGAAA TAACCCCCAC CCTGAAAGAG 
CTAAAAACAC AGAGCCAAAG TGTCTACAAT AATCTAGTGA GTTTTGCATC ACCCTTAGTC 
ACGGATACAT CAAATGAATG CTCAAGCCCA ACATCAGCTG CTACATACCA GCCAACCTTT 
GCTGCAGCTG TACGTAGCAA TACAGGGCAG AAAACTCAGC CAGAGGTGAT GTCACAGAAT 
GCAAGAAAGC TAATTCAGAA AAATCTTGCC ACATCAGCAG ATACTCCTCC AAGCACAGTC 
CCAGGAACTG GCAAAAGTGT TGCCTGTAGT CCCAAGAAGA CAGCCAGGGA CCCTAAAACA 
GGAAAAGCTG TGCAGGAAAG GAATTCATAT GCAGTTAGTG TGTGGAAACG AGTCAAAGCC 
AAGTTGGAGG GCCGAGATGT GGATCCCAGC AGGAGAATGT CTGTTGCTGA ACAGGTTGAC 
TTTGTGATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACT 
GCCTGGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RP11-345J4.5 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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