ap4e1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ap4e1
Entrez Gene ID	100494908
Full Name	adaptor related protein complex 4, epsilon 1 subunit
General protein information	Preferred Names adaptor related protein complex 4, epsilon 1 subunit Names AP-4 complex subunit epsilon-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002936344.5	XP_002936390.2	AP-4 complex subunit epsilon-1
XM_002936344.4	XP_002936390.1	AP-4 complex subunit epsilon-1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AP4E1	XP_003586629.1
Rattus norvegicus (Norway rat)	Ap4e1	NP_001178671.1
Xenopus tropicalis (tropical clawed frog)	ap4e1	XP_002936390.1
Homo sapiens (human)	AP4E1	NP_031373.2
Pan troglodytes (chimpanzee)	AP4E1	XP_001169245.1
Mus musculus (house mouse)	Ap4e1	NP_780759.2
Danio rerio (zebrafish)	ap4e1	XP_699042.2
Arabidopsis thaliana (thale cress)	AT1G31730	NP_174454.2
Oryza sativa (rice)	Os01g0916200	NP_001045192.1
Macaca mulatta (Rhesus monkey)	AP4E1	XP_002804828.1
Canis lupus familiaris (dog)	AP4E1	XP_535479.2
Gallus gallus (chicken)	AP4E1	XP_413808.3

Gene Ontology

The following ap4e1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ap4e1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa07358	XM_002936344.4 Latest version!	Xenopus tropicalis adaptor related protein complex 4, epsilon 1 subunit (ap4e1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa55105	XM_002936344.5 Latest version!	Xenopus tropicalis adaptor related protein complex 4 subunit epsilon 1 (ap4e1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ap4e1 ( XM_002936344.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936390.1 Xenopus tropicalis adaptor related protein complex 4, epsilon 1 subunit (ap4e1), mRNA.

Price & Availability↑

CloneID	OXa07358
Clone ID Related Accession (Same CDS sequence)	XM_002936344.4
Accession Version	XM_002936344.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP-4 complex subunit epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936344.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGA TCGTAGAAAG GACTCTCAGC GCGCTGCCTG GCCTTATCCT CCAGGACTCG 
CCGTCCGGGG CCAGTTCCAA GCTCGGAAAC CTCATCCGTA GCATCACGGC TCTCACTTCC 
AGACAGGAAG AAGAAAAATT AATTCAGCAG GAGCTTGCTA ATTTGAAAGT AACCGTTTCC 
TCTGCAAACA CAACTCTGAG GCAGATGAAG GAATGCATGG TGAGATTCAT TTACTGCGAA 
ATGCTGGGAT ATGAGTCTGG ATTTGGTTAC ATTCACGCCA TCAAGTTGGC TCAACAAGGG 
AATTTGCTTG AAAAAAGAGT AGGGTACCTG GCAGTGTCCT TATTCCTGCA TGAAAACCAT 
GAATTATTGC TTTTGTTGGT GAATACAGTT TTAAAGGACC TACAAAGCAC CAACCTTGTT 
GAAGTATGCA TGGCACTGAC AGTTGTCAGC CAAATCTTTC CAAAGGAAAT GATTCCAGCT 
GCCCTGCCAC TGATAGAAGA CAAGCTGCAG CATTCAAAAG AAATCGTGAG GAGGAAAGCT 
GTTCTGGCAT TATATAAATT CTATATGATA GCACCCACTC AAGTCCAGCA CATCCATGAG 
AAGTTCAGGA AAGCACTTTG TGACCGGGAT GTTGGAGTCA TGGCTGCTTC ACTGCACATA 
TATTACCAAG TGATCAAGGA CAACCCAGCT GGTTACAAAG ACCTGACAGG TTCTTTCATT 
ACCATTTTAA AGCAAGTGGT TGGAGGGAAA CTGCCTGCAG ATTTTAATTA CCATAGTGTC 
CCAGCACCAT GGCTTCAGAT TCAGCTCCTG AGGATTTTAA GATTACTGGG AAAAGATGAC 
CCCAGGGCCA GTGAGCTAAT CTATGATGTG TTGGATGAAT CCTTAAGGAG GGCAGAAATA 
AATCACAACA TCACCTATGC AATTTTGTTT GAGTGTGTGC AAACCATTTA TACTATTTAC 
CCGAAAGCAG ATTTATTTGA AAAGGCAGCA AAATGTATTG GAAAATTTGT GCTGTCCCCA 
AAGATCAACC TTAAGTATTT AGGTTTAAAG GCACTCACAT ATGTTATCCA ACAGGATCCT 
AATCTGGCAC TTCAGCATCA GATGACAATA ATTGAGTGTT TGGACCACCC AGATCCCATC 
ATTAAAAGAG AGACTCTGGA GCTGCTGTAC CGAATCACTA ATGGCCAGAA CGTGACTGTT 
ATTGTACAAA AGATGTTGGA CTATCTGACG CAGAGTAAAG ATGAATGTAC AATAATTGCC 
TTAGTTGGCA AAATAGCTGA CCTAGCAGAA AAATATGCCC CGAACAATCA GTGGTTTATT 
CAGACAATGA ATGTAGTGTT CTCTATTGGA GGGGACGTGA TACACCCAGA CATACCTAAC 
AACTTCCTTC GACTCCTGGC TGAAGGATTT GATGATGAAA AGGAAGATAG GCAGCTGAGG 
CTTTATGCAG TGCAGTCTTA TCTTGCCTTG CTGGAAACCA AGAATGCTCA TTACCCCCAG 
CGCTTCCTTC AGGTTATCAG TTGGGTACTT GGAGAATATG CCTACCTTGC AAAACATATA 
CCTGTAGAAT CTGTTATGAC AAACCTTTAT GAAGTTCTCA AACAAAGCTC AGTTACTTCT 
GAAACCAAAG CGTGGATTGT TGCAGCTATT AGCAAACTTA CAGCCCTCAG TCCACACTGT 
TCTTTGGTTG ACAGAATAAT CCACGAGTTT AGCACTTGTC CGGACACAGT AATGAGGCAG 
CACGTGTGCG AATTGAAACT GCTGCGAGTG GAGGGCCCCC TAACGCACAA ACTCTTTCCA 
CTCAACTCCA GCTGTGAAGA TTTTATGGTG GATGCCTCAT TGTCCTTTCT GGATGGGTTT 
GTTGCAGATG CACTCAGCAA TGGTGCAGCA ACTTATATTC CCCGTCATCA GCGCCAAGAA 
GAGAAGATGT CTCGTGAAAA AGCTTTGAAT CTGCAACCAT ATGGGCTGTG CTTCTCGCCT 
AGCTTGCCTT CAGCCAGTCT CACGGATGGA CATTCTCCTT CTGCAGTTTC TGTCTTGTCA 
GGAATCTCAG GGAACAGTGG TGAAACTGGG CAAAAAGAGG ATACTCGCCT TAAGCTTGAT 
GGTGTGAAGA AACTTTGGGG TAAAGATGGC TACCTATTGA AAAAGGAAAG TACATCAGTT 
ACATCAGTTA AGCCAGAATC TATGGCTGTC CCTCTGGACA GTGGGATTTC ACAAAGTGGT 
GACAAGAAAC TTCAAAGGAC TGATGAGAGT TTGGTGCCCT TTACAGAGGA AGAGAAAGAG 
AAGAGGCAGC TAGCATCTAC TTTATTTGTT GGCCTTGGAT CCAGTGCTGT CAGCTTGATG 
GGTAAAGCAG AGCAGACTCC AAAAAAGTTC AAGAGGAAAT CAAAAATAGG TGGAAATAAA 
GTGATGAATG AAGAGTCGTC TTCCCTTCCC AGCCATCTCA TTTCTGATGG AGAGGGCTAC 
ACTCCGCCAA CAAGTGGCAC GCACTTACTG AACTCAGATT CCCATACATC ACCTGAGAGA 
CTTGATGCTT TAGGGGAAGG GGATGTGTCT ACTGCTTTGT TTGCCAACTC CAATATGGAT 
ATAATCAAGC CCTTGCAAAG CACCTCACCT AACCAAGGAA CTTGCAGTGT GTACCATGTG 
CCATCCCTGC TGCCTGATCT GGCCCAGCAC CCTCATTCAC CTGTTAAAGA GAGTGCAAGC 
GATGAGAATT TAAGTATATA CTTTAGCAAA GTATGGAAAG ACGACAATGT GCTACTTGCC 
ATAATCATTG TCAATAGGAC TTCTTTAGTA TTTCAGAATA TTAGTGTTCA TTTTGATGAT 
TCAAAAAACT GCACAATTTC CTCAAGCCTT CATTGCCAGC TTTCCCAGGT TGATGCCCTT 
GGTGTGCAGC AGTGTTGGGG CTGTGTACAG ATGTTGATCC CTTGTTCTGC AGAATCAGTG 
TCTGGCTGCA TCAGTTACCA GTCAGAGCTG GCAGAGCCTT GTCAGATCAT CTTTTGTGTA 
GATTTCCTTC TGCTGGATTT TATAAGACCT TTAAGTATTT CAACTGAAGA ATTTGGCATA 
ATGTGGCCTT CTCACTCCAA TGAACTGAAA CGCAAGCTTA CTGTTGGCCA GCAATCATTC 
ACAGGAACTC TTCACAACTT ACAGAGGGAA ATTAACTTTC ATATTGTTGA AATTATAGGT 
GACGAGGGGT TAGCTGCATG CCAGATGCTC CCCACAGTGC CCTGCCTGCT GCATTGTCAC 
TTCAGTTCTG GACTTCTGAC ATTATGGATC AGATCGCATT CTTCCACGTT GCTTGACTGT 
TTGCTACGTC AGTGCCAAAA GGCATTAGAA GAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936390.1
CDS	98..3436
Translation	MSEIVERTLSALPGLILQDSPSGASSKLGNLIRSITALTSRQEEEKLIQQELANLKVTVSSANTTLRQMKECMVRFIYCEMLGYESGFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHENHELLLLLVNTVLKDLQSTNLVEVCMALTVVSQIFPKEMIPAALPLIEDKLQHSKEIVRRKAVLALYKFYMIAPTQVQHIHEKFRKALCDRDVGVMAASLHIYYQVIKDNPAGYKDLTGSFITILKQVVGGKLPADFNYHSVPAPWLQIQLLRILRLLGKDDPRASELIYDVLDESLRRAEINHNITYAILFECVQTIYTIYPKADLFEKAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPNLALQHQMTIIECLDHPDPIIKRETLELLYRITNGQNVTVIVQKMLDYLTQSKDECTIIALVGKIADLAEKYAPNNQWFIQTMNVVFSIGGDVIHPDIPNNFLRLLAEGFDDEKEDRQLRLYAVQSYLALLETKNAHYPQRFLQVISWVLGEYAYLAKHIPVESVMTNLYEVLKQSSVTSETKAWIVAAISKLTALSPHCSLVDRIIHEFSTCPDTVMRQHVCELKLLRVEGPLTHKLFPLNSSCEDFMVDASLSFLDGFVADALSNGAATYIPRHQRQEEKMSREKALNLQPYGLCFSPSLPSASLTDGHSPSAVSVLSGISGNSGETGQKEDTRLKLDGVKKLWGKDGYLLKKESTSVTSVKPESMAVPLDSGISQSGDKKLQRTDESLVPFTEEEKEKRQLASTLFVGLGSSAVSLMGKAEQTPKKFKRKSKIGGNKVMNEESSSLPSHLISDGEGYTPPTSGTHLLNSDSHTSPERLDALGEGDVSTALFANSNMDIIKPLQSTSPNQGTCSVYHVPSLLPDLAQHPHSPVKESASDENLSIYFSKVWKDDNVLLAIIIVNRTSLVFQNISVHFDDSKNCTISSSLHCQLSQVDALGVQQCWGCVQMLIPCSAESVSGCISYQSELAEPCQIIFCVDFLLLDFIRPLSISTEEFGIMWPSHSNELKRKLTVGQQSFTGTLHNLQREINFHIVEIIGDEGLAACQMLPTVPCLLHCHFSSGLLTLWIRSHSSTLLDCLLRQCQKALEEL

Target ORF information:

RefSeq Version	XM_002936344.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis adaptor related protein complex 4, epsilon 1 subunit (ap4e1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936344.4

ORF Insert Sequence:

ATGAGTGAGA TCGTAGAAAG GACTCTCAGC GCGCTGCCTG GCCTTATCCT CCAGGACTCG 
CCGTCCGGGG CCAGTTCCAA GCTCGGAAAC CTCATCCGTA GCATCACGGC TCTCACTTCC 
AGACAGGAAG AAGAAAAATT AATTCAGCAG GAGCTTGCTA ATTTGAAAGT AACCGTTTCC 
TCTGCAAACA CAACTCTGAG GCAGATGAAG GAATGCATGG TGAGATTCAT TTACTGCGAA 
ATGCTGGGAT ATGAGTCTGG ATTTGGTTAC ATTCACGCCA TCAAGTTGGC TCAACAAGGG 
AATTTGCTTG AAAAAAGAGT AGGGTACCTG GCAGTGTCCT TATTCCTGCA TGAAAACCAT 
GAATTATTGC TTTTGTTGGT GAATACAGTT TTAAAGGACC TACAAAGCAC CAACCTTGTT 
GAAGTATGCA TGGCACTGAC AGTTGTCAGC CAAATCTTTC CAAAGGAAAT GATTCCAGCT 
GCCCTGCCAC TGATAGAAGA CAAGCTGCAG CATTCAAAAG AAATCGTGAG GAGGAAAGCT 
GTTCTGGCAT TATATAAATT CTATATGATA GCACCCACTC AAGTCCAGCA CATCCATGAG 
AAGTTCAGGA AAGCACTTTG TGACCGGGAT GTTGGAGTCA TGGCTGCTTC ACTGCACATA 
TATTACCAAG TGATCAAGGA CAACCCAGCT GGTTACAAAG ACCTGACAGG TTCTTTCATT 
ACCATTTTAA AGCAAGTGGT TGGAGGGAAA CTGCCTGCAG ATTTTAATTA CCATAGTGTC 
CCAGCACCAT GGCTTCAGAT TCAGCTCCTG AGGATTTTAA GATTACTGGG AAAAGATGAC 
CCCAGGGCCA GTGAGCTAAT CTATGATGTG TTGGATGAAT CCTTAAGGAG GGCAGAAATA 
AATCACAACA TCACCTATGC AATTTTGTTT GAGTGTGTGC AAACCATTTA TACTATTTAC 
CCGAAAGCAG ATTTATTTGA AAAGGCAGCA AAATGTATTG GAAAATTTGT GCTGTCCCCA 
AAGATCAACC TTAAGTATTT AGGTTTAAAG GCACTCACAT ATGTTATCCA ACAGGATCCT 
AATCTGGCAC TTCAGCATCA GATGACAATA ATTGAGTGTT TGGACCACCC AGATCCCATC 
ATTAAAAGAG AGACTCTGGA GCTGCTGTAC CGAATCACTA ATGGCCAGAA CGTGACTGTT 
ATTGTACAAA AGATGTTGGA CTATCTGACG CAGAGTAAAG ATGAATGTAC AATAATTGCC 
TTAGTTGGCA AAATAGCTGA CCTAGCAGAA AAATATGCCC CGAACAATCA GTGGTTTATT 
CAGACAATGA ATGTAGTGTT CTCTATTGGA GGGGACGTGA TACACCCAGA CATACCTAAC 
AACTTCCTTC GACTCCTGGC TGAAGGATTT GATGATGAAA AGGAAGATAG GCAGCTGAGG 
CTTTATGCAG TGCAGTCTTA TCTTGCCTTG CTGGAAACCA AGAATGCTCA TTACCCCCAG 
CGCTTCCTTC AGGTTATCAG TTGGGTACTT GGAGAATATG CCTACCTTGC AAAACATATA 
CCTGTAGAAT CTGTTATGAC AAACCTTTAT GAAGTTCTCA AACAAAGCTC AGTTACTTCT 
GAAACCAAAG CGTGGATTGT TGCAGCTATT AGCAAACTTA CAGCCCTCAG TCCACACTGT 
TCTTTGGTTG ACAGAATAAT CCACGAGTTT AGCACTTGTC CGGACACAGT AATGAGGCAG 
CACGTGTGCG AATTGAAACT GCTGCGAGTG GAGGGCCCCC TAACGCACAA ACTCTTTCCA 
CTCAACTCCA GCTGTGAAGA TTTTATGGTG GATGCCTCAT TGTCCTTTCT GGATGGGTTT 
GTTGCAGATG CACTCAGCAA TGGTGCAGCA ACTTATATTC CCCGTCATCA GCGCCAAGAA 
GAGAAGATGT CTCGTGAAAA AGCTTTGAAT CTGCAACCAT ATGGGCTGTG CTTCTCGCCT 
AGCTTGCCTT CAGCCAGTCT CACGGATGGA CATTCTCCTT CTGCAGTTTC TGTCTTGTCA 
GGAATCTCAG GGAACAGTGG TGAAACTGGG CAAAAAGAGG ATACTCGCCT TAAGCTTGAT 
GGTGTGAAGA AACTTTGGGG TAAAGATGGC TACCTATTGA AAAAGGAAAG TACATCAGTT 
ACATCAGTTA AGCCAGAATC TATGGCTGTC CCTCTGGACA GTGGGATTTC ACAAAGTGGT 
GACAAGAAAC TTCAAAGGAC TGATGAGAGT TTGGTGCCCT TTACAGAGGA AGAGAAAGAG 
AAGAGGCAGC TAGCATCTAC TTTATTTGTT GGCCTTGGAT CCAGTGCTGT CAGCTTGATG 
GGTAAAGCAG AGCAGACTCC AAAAAAGTTC AAGAGGAAAT CAAAAATAGG TGGAAATAAA 
GTGATGAATG AAGAGTCGTC TTCCCTTCCC AGCCATCTCA TTTCTGATGG AGAGGGCTAC 
ACTCCGCCAA CAAGTGGCAC GCACTTACTG AACTCAGATT CCCATACATC ACCTGAGAGA 
CTTGATGCTT TAGGGGAAGG GGATGTGTCT ACTGCTTTGT TTGCCAACTC CAATATGGAT 
ATAATCAAGC CCTTGCAAAG CACCTCACCT AACCAAGGAA CTTGCAGTGT GTACCATGTG 
CCATCCCTGC TGCCTGATCT GGCCCAGCAC CCTCATTCAC CTGTTAAAGA GAGTGCAAGC 
GATGAGAATT TAAGTATATA CTTTAGCAAA GTATGGAAAG ACGACAATGT GCTACTTGCC 
ATAATCATTG TCAATAGGAC TTCTTTAGTA TTTCAGAATA TTAGTGTTCA TTTTGATGAT 
TCAAAAAACT GCACAATTTC CTCAAGCCTT CATTGCCAGC TTTCCCAGGT TGATGCCCTT 
GGTGTGCAGC AGTGTTGGGG CTGTGTACAG ATGTTGATCC CTTGTTCTGC AGAATCAGTG 
TCTGGCTGCA TCAGTTACCA GTCAGAGCTG GCAGAGCCTT GTCAGATCAT CTTTTGTGTA 
GATTTCCTTC TGCTGGATTT TATAAGACCT TTAAGTATTT CAACTGAAGA ATTTGGCATA 
ATGTGGCCTT CTCACTCCAA TGAACTGAAA CGCAAGCTTA CTGTTGGCCA GCAATCATTC 
ACAGGAACTC TTCACAACTT ACAGAGGGAA ATTAACTTTC ATATTGTTGA AATTATAGGT 
GACGAGGGGT TAGCTGCATG CCAGATGCTC CCCACAGTGC CCTGCCTGCT GCATTGTCAC 
TTCAGTTCTG GACTTCTGAC ATTATGGATC AGATCGCATT CTTCCACGTT GCTTGACTGT 
TTGCTACGTC AGTGCCAAAA GGCATTAGAA GAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ap4e1 ( XM_002936344.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936390.2 Xenopus tropicalis adaptor related protein complex 4 subunit epsilon 1 (ap4e1), mRNA.

Price & Availability↑

CloneID	OXa55105
Clone ID Related Accession (Same CDS sequence)	XM_002936344.5
Accession Version	XM_002936344.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP-4 complex subunit epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936344.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGA TCGTAGAAAG GACTCTCAGC GCGCTGCCTG GCCTTATCCT CCAGGACTCG 
CCGTCCGGGG CCAGTTCCAA GCTCGGAAAC CTCATCCGTA GCATCACGGC TCTCACTTCC 
AGACAGGAAG AAGAAAAATT AATTCAGCAG GAGCTTGCTA ATTTGAAAGT AACCGTTTCC 
TCTGCAAACA CAACTCTGAG GCAGATGAAG GAATGCATGG TGAGATTCAT TTACTGCGAA 
ATGCTGGGAT ATGAGTCTGG ATTTGGTTAC ATTCACGCCA TCAAGTTGGC TCAACAAGGG 
AATTTGCTTG AAAAAAGAGT AGGGTACCTG GCAGTGTCCT TATTCCTGCA TGAAAACCAT 
GAATTATTGC TTTTGTTGGT GAATACAGTT TTAAAGGACC TACAAAGCAC CAACCTTGTT 
GAAGTATGCA TGGCACTGAC AGTTGTCAGC CAAATCTTTC CAAAGGAAAT GATTCCAGCT 
GCCCTGCCAC TGATAGAAGA CAAGCTGCAG CATTCAAAAG AAATCGTGAG GAGGAAAGCT 
GTTCTGGCAT TATATAAATT CTATATGATA GCACCCACTC AAGTCCAGCA CATCCATGAG 
AAGTTCAGGA AAGCACTTTG TGACCGGGAT GTTGGAGTCA TGGCTGCTTC ACTGCACATA 
TATTACCAAG TGATCAAGGA CAACCCAGCT GGTTACAAAG ACCTGACAGG TTCTTTCATT 
ACCATTTTAA AGCAAGTGGT TGGAGGGAAA CTGCCTGCAG ATTTTAATTA CCATAGTGTC 
CCAGCACCAT GGCTTCAGAT TCAGCTCCTG AGGATTTTAA GATTACTGGG AAAAGATGAC 
CCCAGGGCCA GTGAGCTAAT CTATGATGTG TTGGATGAAT CCTTAAGGAG GGCAGAAATA 
AATCACAACA TCACCTATGC AATTTTGTTT GAGTGTGTGC AAACCATTTA TACTATTTAC 
CCGAAAGCAG ATTTATTTGA AAAGGCAGCA AAATGTATTG GAAAATTTGT GCTGTCCCCA 
AAGATCAACC TTAAGTATTT AGGTTTAAAG GCACTCACAT ATGTTATCCA ACAGGATCCT 
AATCTGGCAC TTCAGCATCA GATGACAATA ATTGAGTGTT TGGACCACCC AGATCCCATC 
ATTAAAAGAG AGACTCTGGA GCTGCTGTAC CGAATCACTA ATGGCCAGAA CGTGACTGTT 
ATTGTACAAA AGATGTTGGA CTATCTGACG CAGAGTAAAG ATGAATGTAC AATAATTGCC 
TTAGTTGGCA AAATAGCTGA CCTAGCAGAA AAATATGCCC CGAACAATCA GTGGTTTATT 
CAGACAATGA ATGTAGTGTT CTCTATTGGA GGGGACGTGA TACACCCAGA CATACCTAAC 
AACTTCCTTC GACTCCTGGC TGAAGGATTT GATGATGAAA AGGAAGATAG GCAGCTGAGG 
CTTTATGCAG TGCAGTCTTA TCTTGCCTTG CTGGAAACCA AGAATGCTCA TTACCCCCAG 
CGCTTCCTTC AGGTTATCAG TTGGGTACTT GGAGAATATG CCTACCTTGC AAAACATATA 
CCTGTAGAAT CTGTTATGAC AAACCTTTAT GAAGTTCTCA AACAAAGATC AGTTACTTCT 
GAAACCAAAG CGTGGATTGT TGCAGCTATT AGCAAACTTA CAGCCCTCAG TCCACACTGT 
TCTTTGGTTG ACAGAATAAT CCACGAGTTT AGCACTTGTC CGGACACAGT AATGAGGCAG 
CACGTGTGCG AATTGAAACT GCTGCGAGTG GAGGGCCCCC TAACGCACAA ACTCTTTCCA 
CTCAACTCCA GCTGTGAAGA TTTTATGGTG GATGCCTCAT TGTCCTTTCT GGATGGGTTT 
GTTGCAGATG CACTCAGCAA TGGTGCAGCA ACTTATATTC CCCGTCATCA GCGCCAAGAA 
GAGAAGATGT CTCGTGAAAA AGCTTTGAAT CTGCAACCAT ATGGGCTGTG CTTCTCGCCT 
AGCTTGCCTT CAGCCAGTCT CACGGATGGA CATTCTCCTT CTGCAGTTTC TGTCTCGTCA 
GGAATCTCAG GGAACAGTGG TGAAACTGGG CAAAAAGAGG ATACTCGCCT TAAGCTTGAT 
GGTGTGAAGA AACTTTGGGG TAAAGATGGC TACCTATTGA AAAAGGAAAG TACATCAGTT 
ACATCAGTTA AGCCAGAATC TATGGCTGTC CCTCTGGACA GTGGGATTTC ACAAAGTGGT 
GACAAGAAAC TTCAAAGGAC TGATGAGAGT TTGGTGCCCT TTACAGAGGA AGAGAAAGAG 
AAGAGGCAGC TAGCATCTAC TTTATTTGTT GGCCTTGGAT CCAGTGCTGT CAGCTTGATG 
GGTAAAGCAG AGCAGACTCC AAAAAAGTTC AAGAGGAAAT CAAAAATAGG TGGAAATAAA 
GTGATGAATG AAGAGTCGTC TTCCCTTCCC AGCCATCTCA TTTCTGATGG AGAGGGCTAC 
ACTCCGCCAA CAAGTGGCAC GCACTTACTG AACTCAGATT CCCATACATC ACCTGAGAGA 
CTTGATGCTT TAGGGGAAGG GGATGTGTCT ACTGCTTTGT TTGCCAACTC CAATATGGAT 
ATAATCAAGC CCTTGCAAAG CACCTCACCT AACCAAGGAA CTTGCAGTGT GTACCATGTG 
CCATCCCTGC TGCCTGATCT GGCCCAGCAC CCTCATTCAC CTGTTAAAGA GAGTGCAAGC 
GATGAGAATT TAAGTATATA CTTTAGCAAA GTATGGAAAG ACGACAATGT GCTACTTGCC 
ATAATCATTG TCAATAGGAC TTCTTTAGTA TTTCAGAATA TTAGTGTTCA TTTTGATGAT 
TCAAAAAACT GCACAATTTC CTCAAGCCTT CATTGCCAGC TTTCCCAGGT TGATGCCCTT 
GGTGTGCAGC AGTGTTGGGG CTGTGTACAG ATGTTGATCC CTTGTTCTGC AGAATCAGTG 
TCTGGCTGCA TCAGTTACCA GTCAGAGCTG GCAGAGCCTT GTCAGATCAT CTTTTGTGTA 
GATTTCCTTC TGCTGGATTT TATAAGACCT TTAAGTATTT CAACTGAAGA ATTTGGCATA 
ATGTGGCCTT CTCACTCCAA TGAACTGAAA CGCAAGCTTA CTGTTGGCCA GCAATCATTC 
ACAGGAACTC TTCACAACTT ACAGAGGGAA ATTAACTTTC ATATTGTTGA AATTATAGGT 
GACGAGGGGT TAGCTGCATG CCAGATGCTC CCCACAGTGC CCTGCCTGCT GCATTGTCAC 
TTCAGTTCTG GACTTCTGAC ATTATGGATC AGATCGCATT CATCCACGTT GCTTGACTGT 
TTGCTACGTC AGTGCCAAAA GGCATTAGAA GAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936390.2
CDS	98..3436
Translation	MSEIVERTLSALPGLILQDSPSGASSKLGNLIRSITALTSRQEEEKLIQQELANLKVTVSSANTTLRQMKECMVRFIYCEMLGYESGFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHENHELLLLLVNTVLKDLQSTNLVEVCMALTVVSQIFPKEMIPAALPLIEDKLQHSKEIVRRKAVLALYKFYMIAPTQVQHIHEKFRKALCDRDVGVMAASLHIYYQVIKDNPAGYKDLTGSFITILKQVVGGKLPADFNYHSVPAPWLQIQLLRILRLLGKDDPRASELIYDVLDESLRRAEINHNITYAILFECVQTIYTIYPKADLFEKAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPNLALQHQMTIIECLDHPDPIIKRETLELLYRITNGQNVTVIVQKMLDYLTQSKDECTIIALVGKIADLAEKYAPNNQWFIQTMNVVFSIGGDVIHPDIPNNFLRLLAEGFDDEKEDRQLRLYAVQSYLALLETKNAHYPQRFLQVISWVLGEYAYLAKHIPVESVMTNLYEVLKQRSVTSETKAWIVAAISKLTALSPHCSLVDRIIHEFSTCPDTVMRQHVCELKLLRVEGPLTHKLFPLNSSCEDFMVDASLSFLDGFVADALSNGAATYIPRHQRQEEKMSREKALNLQPYGLCFSPSLPSASLTDGHSPSAVSVSSGISGNSGETGQKEDTRLKLDGVKKLWGKDGYLLKKESTSVTSVKPESMAVPLDSGISQSGDKKLQRTDESLVPFTEEEKEKRQLASTLFVGLGSSAVSLMGKAEQTPKKFKRKSKIGGNKVMNEESSSLPSHLISDGEGYTPPTSGTHLLNSDSHTSPERLDALGEGDVSTALFANSNMDIIKPLQSTSPNQGTCSVYHVPSLLPDLAQHPHSPVKESASDENLSIYFSKVWKDDNVLLAIIIVNRTSLVFQNISVHFDDSKNCTISSSLHCQLSQVDALGVQQCWGCVQMLIPCSAESVSGCISYQSELAEPCQIIFCVDFLLLDFIRPLSISTEEFGIMWPSHSNELKRKLTVGQQSFTGTLHNLQREINFHIVEIIGDEGLAACQMLPTVPCLLHCHFSSGLLTLWIRSHSSTLLDCLLRQCQKALEEL

Target ORF information:

RefSeq Version	XM_002936344.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis adaptor related protein complex 4 subunit epsilon 1 (ap4e1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936344.5

ORF Insert Sequence:

ATGAGTGAGA TCGTAGAAAG GACTCTCAGC GCGCTGCCTG GCCTTATCCT CCAGGACTCG 
CCGTCCGGGG CCAGTTCCAA GCTCGGAAAC CTCATCCGTA GCATCACGGC TCTCACTTCC 
AGACAGGAAG AAGAAAAATT AATTCAGCAG GAGCTTGCTA ATTTGAAAGT AACCGTTTCC 
TCTGCAAACA CAACTCTGAG GCAGATGAAG GAATGCATGG TGAGATTCAT TTACTGCGAA 
ATGCTGGGAT ATGAGTCTGG ATTTGGTTAC ATTCACGCCA TCAAGTTGGC TCAACAAGGG 
AATTTGCTTG AAAAAAGAGT AGGGTACCTG GCAGTGTCCT TATTCCTGCA TGAAAACCAT 
GAATTATTGC TTTTGTTGGT GAATACAGTT TTAAAGGACC TACAAAGCAC CAACCTTGTT 
GAAGTATGCA TGGCACTGAC AGTTGTCAGC CAAATCTTTC CAAAGGAAAT GATTCCAGCT 
GCCCTGCCAC TGATAGAAGA CAAGCTGCAG CATTCAAAAG AAATCGTGAG GAGGAAAGCT 
GTTCTGGCAT TATATAAATT CTATATGATA GCACCCACTC AAGTCCAGCA CATCCATGAG 
AAGTTCAGGA AAGCACTTTG TGACCGGGAT GTTGGAGTCA TGGCTGCTTC ACTGCACATA 
TATTACCAAG TGATCAAGGA CAACCCAGCT GGTTACAAAG ACCTGACAGG TTCTTTCATT 
ACCATTTTAA AGCAAGTGGT TGGAGGGAAA CTGCCTGCAG ATTTTAATTA CCATAGTGTC 
CCAGCACCAT GGCTTCAGAT TCAGCTCCTG AGGATTTTAA GATTACTGGG AAAAGATGAC 
CCCAGGGCCA GTGAGCTAAT CTATGATGTG TTGGATGAAT CCTTAAGGAG GGCAGAAATA 
AATCACAACA TCACCTATGC AATTTTGTTT GAGTGTGTGC AAACCATTTA TACTATTTAC 
CCGAAAGCAG ATTTATTTGA AAAGGCAGCA AAATGTATTG GAAAATTTGT GCTGTCCCCA 
AAGATCAACC TTAAGTATTT AGGTTTAAAG GCACTCACAT ATGTTATCCA ACAGGATCCT 
AATCTGGCAC TTCAGCATCA GATGACAATA ATTGAGTGTT TGGACCACCC AGATCCCATC 
ATTAAAAGAG AGACTCTGGA GCTGCTGTAC CGAATCACTA ATGGCCAGAA CGTGACTGTT 
ATTGTACAAA AGATGTTGGA CTATCTGACG CAGAGTAAAG ATGAATGTAC AATAATTGCC 
TTAGTTGGCA AAATAGCTGA CCTAGCAGAA AAATATGCCC CGAACAATCA GTGGTTTATT 
CAGACAATGA ATGTAGTGTT CTCTATTGGA GGGGACGTGA TACACCCAGA CATACCTAAC 
AACTTCCTTC GACTCCTGGC TGAAGGATTT GATGATGAAA AGGAAGATAG GCAGCTGAGG 
CTTTATGCAG TGCAGTCTTA TCTTGCCTTG CTGGAAACCA AGAATGCTCA TTACCCCCAG 
CGCTTCCTTC AGGTTATCAG TTGGGTACTT GGAGAATATG CCTACCTTGC AAAACATATA 
CCTGTAGAAT CTGTTATGAC AAACCTTTAT GAAGTTCTCA AACAAAGATC AGTTACTTCT 
GAAACCAAAG CGTGGATTGT TGCAGCTATT AGCAAACTTA CAGCCCTCAG TCCACACTGT 
TCTTTGGTTG ACAGAATAAT CCACGAGTTT AGCACTTGTC CGGACACAGT AATGAGGCAG 
CACGTGTGCG AATTGAAACT GCTGCGAGTG GAGGGCCCCC TAACGCACAA ACTCTTTCCA 
CTCAACTCCA GCTGTGAAGA TTTTATGGTG GATGCCTCAT TGTCCTTTCT GGATGGGTTT 
GTTGCAGATG CACTCAGCAA TGGTGCAGCA ACTTATATTC CCCGTCATCA GCGCCAAGAA 
GAGAAGATGT CTCGTGAAAA AGCTTTGAAT CTGCAACCAT ATGGGCTGTG CTTCTCGCCT 
AGCTTGCCTT CAGCCAGTCT CACGGATGGA CATTCTCCTT CTGCAGTTTC TGTCTCGTCA 
GGAATCTCAG GGAACAGTGG TGAAACTGGG CAAAAAGAGG ATACTCGCCT TAAGCTTGAT 
GGTGTGAAGA AACTTTGGGG TAAAGATGGC TACCTATTGA AAAAGGAAAG TACATCAGTT 
ACATCAGTTA AGCCAGAATC TATGGCTGTC CCTCTGGACA GTGGGATTTC ACAAAGTGGT 
GACAAGAAAC TTCAAAGGAC TGATGAGAGT TTGGTGCCCT TTACAGAGGA AGAGAAAGAG 
AAGAGGCAGC TAGCATCTAC TTTATTTGTT GGCCTTGGAT CCAGTGCTGT CAGCTTGATG 
GGTAAAGCAG AGCAGACTCC AAAAAAGTTC AAGAGGAAAT CAAAAATAGG TGGAAATAAA 
GTGATGAATG AAGAGTCGTC TTCCCTTCCC AGCCATCTCA TTTCTGATGG AGAGGGCTAC 
ACTCCGCCAA CAAGTGGCAC GCACTTACTG AACTCAGATT CCCATACATC ACCTGAGAGA 
CTTGATGCTT TAGGGGAAGG GGATGTGTCT ACTGCTTTGT TTGCCAACTC CAATATGGAT 
ATAATCAAGC CCTTGCAAAG CACCTCACCT AACCAAGGAA CTTGCAGTGT GTACCATGTG 
CCATCCCTGC TGCCTGATCT GGCCCAGCAC CCTCATTCAC CTGTTAAAGA GAGTGCAAGC 
GATGAGAATT TAAGTATATA CTTTAGCAAA GTATGGAAAG ACGACAATGT GCTACTTGCC 
ATAATCATTG TCAATAGGAC TTCTTTAGTA TTTCAGAATA TTAGTGTTCA TTTTGATGAT 
TCAAAAAACT GCACAATTTC CTCAAGCCTT CATTGCCAGC TTTCCCAGGT TGATGCCCTT 
GGTGTGCAGC AGTGTTGGGG CTGTGTACAG ATGTTGATCC CTTGTTCTGC AGAATCAGTG 
TCTGGCTGCA TCAGTTACCA GTCAGAGCTG GCAGAGCCTT GTCAGATCAT CTTTTGTGTA 
GATTTCCTTC TGCTGGATTT TATAAGACCT TTAAGTATTT CAACTGAAGA ATTTGGCATA 
ATGTGGCCTT CTCACTCCAA TGAACTGAAA CGCAAGCTTA CTGTTGGCCA GCAATCATTC 
ACAGGAACTC TTCACAACTT ACAGAGGGAA ATTAACTTTC ATATTGTTGA AATTATAGGT 
GACGAGGGGT TAGCTGCATG CCAGATGCTC CCCACAGTGC CCTGCCTGCT GCATTGTCAC 
TTCAGTTCTG GACTTCTGAC ATTATGGATC AGATCGCATT CATCCACGTT GCTTGACTGT 
TTGCTACGTC AGTGCCAAAA GGCATTAGAA GAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ap4e1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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