AP4E1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	AP4E1
Entrez Gene ID	478304
Full Name	adaptor related protein complex 4 epsilon 1 subunit
General protein information	Preferred Names adaptor related protein complex 4 epsilon 1 subunit Names AP-4 complex subunit epsilon-1
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022412570.1	XP_022268278.1	AP-4 complex subunit epsilon-1 isoform X1
XM_022412571.1	XP_022268279.1	AP-4 complex subunit epsilon-1 isoform X2
XM_535479.5	XP_535479.2	AP-4 complex subunit epsilon-1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AP4E1	XP_003586629.1
Rattus norvegicus (Norway rat)	Ap4e1	NP_001178671.1
Xenopus tropicalis (tropical clawed frog)	ap4e1	XP_002936390.1
Homo sapiens (human)	AP4E1	NP_031373.2
Pan troglodytes (chimpanzee)	AP4E1	XP_001169245.1
Mus musculus (house mouse)	Ap4e1	NP_780759.2
Danio rerio (zebrafish)	ap4e1	XP_699042.2
Arabidopsis thaliana (thale cress)	AT1G31730	NP_174454.2
Oryza sativa (rice)	Os01g0916200	NP_001045192.1
Macaca mulatta (Rhesus monkey)	AP4E1	XP_002804828.1
Canis lupus familiaris (dog)	AP4E1	XP_535479.2
Gallus gallus (chicken)	AP4E1	XP_413808.3

Gene Ontology

The following AP4E1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP4E1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe28858	XM_022412570.1 Latest version!	Canis lupus familiaris adaptor related protein complex 4 epsilon 1 subunit (AP4E1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe28858	XM_535479.5 Latest version!	Canis lupus familiaris adaptor-related protein complex 4, epsilon 1 subunit (AP4E1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe28860	XM_022412571.1 Latest version!	Canis lupus familiaris adaptor related protein complex 4 epsilon 1 subunit (AP4E1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP4E1 ( XM_022412570.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022268278.1 Canis lupus familiaris adaptor related protein complex 4 epsilon 1 subunit (AP4E1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe28858
Clone ID Related Accession (Same CDS sequence)	XM_022412570.1 , XM_535479.5
Accession Version	XM_022412570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	AP-4 complex subunit epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACA TAGTGGAGAA GACGCTGACG GCGCTGCCGG GACTCTTTTT GCAGAACCAG 
TCGGTGGGCG GGCCCGCGGG CGTCAAGGCT TCTTTCTCTT CGCGGCTGGG CGGCCTCGTC 
CGCGGCATCA CAGCGCTAAC CTCCAAGCAC GAGGAAGAGA AACTAATCCA ACAGGAATTG 
AGTAATCTGA AAGCAACTGT TTCTGCTCCT ACTACAACAC TGAAAATGAT GAAGGAATGT 
ATGGTGAGAC TTATATATTG TGAAATGCTT GGATATGACG CTTCATTTGG CTATATACAT 
GCAATCAAGT TGGCACAACA AGGAAACCTT TTAGAAAAAA GAGTAGGTTA TTTGGCTGTT 
TCTTTATTTT TACATGAAAG TCATGAACTG CTGCTTCTCC TTGTGAATAC AGTTGTAAAG 
GATTTGCAGA GCACTAATCT GGTAGAAGTA TGTATGGCAC TTACTATTGT CAGCCAGATT 
TTCCCTCGAG AAATGATTCC AGCTGTTCTT CCATTAATTG AAGATAAACT TCAACATTCT 
AAGGAGATTA TACGAAGAAA AGCTGTTCTG GCATTATATA AATTCCATCT TATTGCTCCT 
AATCAAGTAC AGCATATCCA TATTAAGTTT CGGAAAGCAC TTTGTGACAG AGATGTTGGG 
GTCATGGCTG CTTCTTTGCA CATATACTTT AGGATGATTA AGGAGAATTC ATCTGGATAT 
AAAGACTTGA CGGGGAGTTT TGTGACAATT TTGAAGCAAG TAGTTGGAGG AAAGCTCCCA 
GTAGATTTCA ATTACCACAG TGTGCCAGCA CCATGGTTAC AAATCCAGCT CTTGAGAATA 
TTAGGACTAC TAGGAAAAGA TGATCAAAGG ACAAGTGAAT TAATGTATGA TGTTCTTGAT 
GAATCCTTAC GAAGAGCCGA GTTAAATCAC AATGTCACAT ATGCGATTCT GTTTGAATGT 
GTGCATACAG TTTATTCTAT TTATCCTAAA TCAGAATTAC TTGAGAAGGC TGCCAAGTGC 
ATTGGAAAAT TTGTTCTTTC ACCTAAAATA AATCTCAAGT ATTTAGGACT GAAGGCTCTT 
ACCTATGTTA TCCAGCAGGA TCCCACTTTG GCTCTTCAGC ACCAGATGAC AATAATTGAA 
TGCTTAGACC ATCCTGATCC CATTATTAAA AGAGAGACAC TGGAACTTCT TTACAGAATT 
ACTAATGCAC AGAATATAAC AGTGATTGTC CAGAAAATGC TTGAATATTT ACATCAGAGC 
AAAGAAGAAT ATATTATTGT CAATTTGGTT GGCAAAATAG CTGAGCTTGC TGAGAAATAT 
GCCCCTGATA ATGCATGGTT TATTCAGACC ATGAATGCTG TGTTTTCAGT GGGAGGAGAT 
GTCATGCATC CTGATATTCC CAATAACTTT TTGAGACTGC TGGCGGAAGG TTTTGATGAT 
GAAACAGAAG ACCAGCAATT AAGGCTTTAT GCAGTTCAGT CTTATCTCAC TTTACTAGAT 
GTGGAAAATG TGTTCTACCC ACAGAAATTT CTTCAAGTTA TGAGTTGGGT GTTAGGGGAA 
TATTCCTACC TCTTAGATAA AGACACACCA GAGGAAGTTC TAACCAAGCT CTACAAGTTA 
CTTATGAATG ACTCCGTTTC TTCAGAAACA AAAGCCTGGT TAATCGCAGC TGTTACCAAG 
CTGACGCCCC AAGCACACTC TTCTAACATA GTCGAGAGAT TAATCCAGGA ATTCACCATA 
TCTTTGGATA CTTGTATGAG ACAGCATGCA TTTGAATTGA AACACTTACG TGAGAATTTG 
GAACTTATGA AGAGTTTGCT TCCAGTTGAT AAAAGCTGTG AAGACATGGT GGTAGATGCT 
TCTTTATCTT TTCTGGATGG TTTTGTGGCT GAAGAACTTA GTCAGGGGGC AGCACCTTAC 
AAACCTCACC ACCAACGGCA GGAGGAAAAG CTTTCTCAGG AAAAAGTTCT CAATTTTGAA 
CCATATGGAC TCTCCTTTTC TTCATCTGGC TTCACTGGAC GACAGTCTCC TGCTGGCATT 
TCCCTTGGTT CAGATCTATC TGGGAATAGT GCAGAGACTG GACTGAAGGA GACAAATAGC 
TTGAAGCTGG AAGGTATAAA GAAATTGTGG GGGAAAGAGG GCTATCTTCC TAAGAAGGAA 
GGTAAAACTG GGGATGAAAC TGAAGCTCTG CCTGTTCCTC AAGAGAGCAT AATAATGGAG 
AATGTAGACC AAACTATAAC AAAAAAGGAT CAGTGTCAAG TCCTTACCCA ATCTAAAGAG 
GAGAAAGAAA AGCAGCTGCT GGCATCATCG TTATTTGTTG GGCTAGGATC AGAAAGTACA 
ATCAATTTGC TAGGAAAAGC AGATACTGTA TCTCACAAAT TCAGAAGGAA ATCAAAAGTC 
AAGGAAACCA AAAGTGGAGA AACAAGCAGT GCTCATAATA TCACCTGTTC TTACTTTAGT 
TCTTCGTCAA ATGCAACTTA TGAAGATGAT TATTATTTGG ATACTTTGCA GGATAGAGAA 
GACAAAGAAT TAAATAAATT TTCTCTCAAT TCTGAACTTT TGGATTCTGA GTCACTCACA 
GAACTACCTT CAGTTGAGAA ACTCTCCAAT TGCAAGCTGT CTTCACCATC TTTGTTTACA 
GATAACAACA TGGAAGGTTT TCACCCTTCT CAGCCCACTG CACCCTCAGT TGCTAATGAA 
ACCTCTTTAG CTTCTTCTTT ACTGGAAGAA ACTTCAGAGC ACATGCATTC AGATCTTGTG 
GAGGTCTGTC ATAATGAAAC CATATCAATG TCTTCTTACA AAATTTGGAA AGATGATTGT 
TTATTGATGG TATGGTCAGT TTCTAATAAG AGTGGTTTGG AATTGAAAAG TGCTAACTTA 
GAAATTGCTC CAGCAGAATA TTTCAAGATC ACTGAGCAAC CTGGATGCTG CTTGCCTGTA 
ATAGAAGCAG AAAGCATCAA AACCTTTCAG TATAATGTGC AGATGGAGAA ACCTTTTACA 
GACGGGAATC TTACTGGTTT TATAAATTAT CAAATGATGG ATACTCATTC TGTTCAGCTT 
GAATTTTCTG TAAACTTATC GCTATTAGAT TTCATTAGAC CGTTAAAAAT CTCAACTGAA 
AACTTTGGCA AACTCTGGTT ATCCTTCGCA AATGATGTGA AGCAAAATGT AAAAGTGTCA 
GAATCTCAAG CTGCTCTGTC TTCTGCCCTA AAGACCCTGC AACAGAAACT AAGACTTCAT 
GTTGTTGAAA TTATAGGCAA CGAAGGGCTT TTGGCCTGTC AACTGCTCCC ATCCATCCCC 
TGTTTGCTGC ACTTTCGAGT TCATGGTGAT GTATTGGCCT TGTGGTTCAG ATCCTCCTGC 
TCTTCTCTTC CTGACTACTT ACTGTATCAG TGTCAAAAGG TGATGGAGGG ATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268278.1
CDS	63..3479
Translation	MSDIVEKTLTALPGLFLQNQSVGGPAGVKASFSSRLGGLVRGITALTSKHEEEKLIQQELSNLKATVSAPTTTLKMMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTIVSQIFPREMIPAVLPLIEDKLQHSKEIIRRKAVLALYKFHLIAPNQVQHIHIKFRKALCDRDVGVMAASLHIYFRMIKENSSGYKDLTGSFVTILKQVVGGKLPVDFNYHSVPAPWLQIQLLRILGLLGKDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNITVIVQKMLEYLHQSKEEYIIVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLYAVQSYLTLLDVENVFYPQKFLQVMSWVLGEYSYLLDKDTPEEVLTKLYKLLMNDSVSSETKAWLIAAVTKLTPQAHSSNIVERLIQEFTISLDTCMRQHAFELKHLRENLELMKSLLPVDKSCEDMVVDASLSFLDGFVAEELSQGAAPYKPHHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDLSGNSAETGLKETNSLKLEGIKKLWGKEGYLPKKEGKTGDETEALPVPQESIIMENVDQTITKKDQCQVLTQSKEEKEKQLLASSLFVGLGSESTINLLGKADTVSHKFRRKSKVKETKSGETSSAHNITCSYFSSSSNATYEDDYYLDTLQDREDKELNKFSLNSELLDSESLTELPSVEKLSNCKLSSPSLFTDNNMEGFHPSQPTAPSVANETSLASSLLEETSEHMHSDLVEVCHNETISMSSYKIWKDDCLLMVWSVSNKSGLELKSANLEIAPAEYFKITEQPGCCLPVIEAESIKTFQYNVQMEKPFTDGNLTGFINYQMMDTHSVQLEFSVNLSLLDFIRPLKISTENFGKLWLSFANDVKQNVKVSESQAALSSALKTLQQKLRLHVVEIIGNEGLLACQLLPSIPCLLHFRVHGDVLALWFRSSCSSLPDYLLYQCQKVMEGS

Target ORF information:

RefSeq Version	XM_022412570.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adaptor related protein complex 4 epsilon 1 subunit (AP4E1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412570.1

ORF Insert Sequence:

ATGAGCGACA TAGTGGAGAA GACGCTGACG GCGCTGCCGG GACTCTTTTT GCAGAACCAG 
TCGGTGGGCG GGCCCGCGGG CGTCAAGGCT TCTTTCTCTT CGCGGCTGGG CGGCCTCGTC 
CGCGGCATCA CAGCGCTAAC CTCCAAGCAC GAGGAAGAGA AACTAATCCA ACAGGAATTG 
AGTAATCTGA AAGCAACTGT TTCTGCTCCT ACTACAACAC TGAAAATGAT GAAGGAATGT 
ATGGTGAGAC TTATATATTG TGAAATGCTT GGATATGACG CTTCATTTGG CTATATACAT 
GCAATCAAGT TGGCACAACA AGGAAACCTT TTAGAAAAAA GAGTAGGTTA TTTGGCTGTT 
TCTTTATTTT TACATGAAAG TCATGAACTG CTGCTTCTCC TTGTGAATAC AGTTGTAAAG 
GATTTGCAGA GCACTAATCT GGTAGAAGTA TGTATGGCAC TTACTATTGT CAGCCAGATT 
TTCCCTCGAG AAATGATTCC AGCTGTTCTT CCATTAATTG AAGATAAACT TCAACATTCT 
AAGGAGATTA TACGAAGAAA AGCTGTTCTG GCATTATATA AATTCCATCT TATTGCTCCT 
AATCAAGTAC AGCATATCCA TATTAAGTTT CGGAAAGCAC TTTGTGACAG AGATGTTGGG 
GTCATGGCTG CTTCTTTGCA CATATACTTT AGGATGATTA AGGAGAATTC ATCTGGATAT 
AAAGACTTGA CGGGGAGTTT TGTGACAATT TTGAAGCAAG TAGTTGGAGG AAAGCTCCCA 
GTAGATTTCA ATTACCACAG TGTGCCAGCA CCATGGTTAC AAATCCAGCT CTTGAGAATA 
TTAGGACTAC TAGGAAAAGA TGATCAAAGG ACAAGTGAAT TAATGTATGA TGTTCTTGAT 
GAATCCTTAC GAAGAGCCGA GTTAAATCAC AATGTCACAT ATGCGATTCT GTTTGAATGT 
GTGCATACAG TTTATTCTAT TTATCCTAAA TCAGAATTAC TTGAGAAGGC TGCCAAGTGC 
ATTGGAAAAT TTGTTCTTTC ACCTAAAATA AATCTCAAGT ATTTAGGACT GAAGGCTCTT 
ACCTATGTTA TCCAGCAGGA TCCCACTTTG GCTCTTCAGC ACCAGATGAC AATAATTGAA 
TGCTTAGACC ATCCTGATCC CATTATTAAA AGAGAGACAC TGGAACTTCT TTACAGAATT 
ACTAATGCAC AGAATATAAC AGTGATTGTC CAGAAAATGC TTGAATATTT ACATCAGAGC 
AAAGAAGAAT ATATTATTGT CAATTTGGTT GGCAAAATAG CTGAGCTTGC TGAGAAATAT 
GCCCCTGATA ATGCATGGTT TATTCAGACC ATGAATGCTG TGTTTTCAGT GGGAGGAGAT 
GTCATGCATC CTGATATTCC CAATAACTTT TTGAGACTGC TGGCGGAAGG TTTTGATGAT 
GAAACAGAAG ACCAGCAATT AAGGCTTTAT GCAGTTCAGT CTTATCTCAC TTTACTAGAT 
GTGGAAAATG TGTTCTACCC ACAGAAATTT CTTCAAGTTA TGAGTTGGGT GTTAGGGGAA 
TATTCCTACC TCTTAGATAA AGACACACCA GAGGAAGTTC TAACCAAGCT CTACAAGTTA 
CTTATGAATG ACTCCGTTTC TTCAGAAACA AAAGCCTGGT TAATCGCAGC TGTTACCAAG 
CTGACGCCCC AAGCACACTC TTCTAACATA GTCGAGAGAT TAATCCAGGA ATTCACCATA 
TCTTTGGATA CTTGTATGAG ACAGCATGCA TTTGAATTGA AACACTTACG TGAGAATTTG 
GAACTTATGA AGAGTTTGCT TCCAGTTGAT AAAAGCTGTG AAGACATGGT GGTAGATGCT 
TCTTTATCTT TTCTGGATGG TTTTGTGGCT GAAGAACTTA GTCAGGGGGC AGCACCTTAC 
AAACCTCACC ACCAACGGCA GGAGGAAAAG CTTTCTCAGG AAAAAGTTCT CAATTTTGAA 
CCATATGGAC TCTCCTTTTC TTCATCTGGC TTCACTGGAC GACAGTCTCC TGCTGGCATT 
TCCCTTGGTT CAGATCTATC TGGGAATAGT GCAGAGACTG GACTGAAGGA GACAAATAGC 
TTGAAGCTGG AAGGTATAAA GAAATTGTGG GGGAAAGAGG GCTATCTTCC TAAGAAGGAA 
GGTAAAACTG GGGATGAAAC TGAAGCTCTG CCTGTTCCTC AAGAGAGCAT AATAATGGAG 
AATGTAGACC AAACTATAAC AAAAAAGGAT CAGTGTCAAG TCCTTACCCA ATCTAAAGAG 
GAGAAAGAAA AGCAGCTGCT GGCATCATCG TTATTTGTTG GGCTAGGATC AGAAAGTACA 
ATCAATTTGC TAGGAAAAGC AGATACTGTA TCTCACAAAT TCAGAAGGAA ATCAAAAGTC 
AAGGAAACCA AAAGTGGAGA AACAAGCAGT GCTCATAATA TCACCTGTTC TTACTTTAGT 
TCTTCGTCAA ATGCAACTTA TGAAGATGAT TATTATTTGG ATACTTTGCA GGATAGAGAA 
GACAAAGAAT TAAATAAATT TTCTCTCAAT TCTGAACTTT TGGATTCTGA GTCACTCACA 
GAACTACCTT CAGTTGAGAA ACTCTCCAAT TGCAAGCTGT CTTCACCATC TTTGTTTACA 
GATAACAACA TGGAAGGTTT TCACCCTTCT CAGCCCACTG CACCCTCAGT TGCTAATGAA 
ACCTCTTTAG CTTCTTCTTT ACTGGAAGAA ACTTCAGAGC ACATGCATTC AGATCTTGTG 
GAGGTCTGTC ATAATGAAAC CATATCAATG TCTTCTTACA AAATTTGGAA AGATGATTGT 
TTATTGATGG TATGGTCAGT TTCTAATAAG AGTGGTTTGG AATTGAAAAG TGCTAACTTA 
GAAATTGCTC CAGCAGAATA TTTCAAGATC ACTGAGCAAC CTGGATGCTG CTTGCCTGTA 
ATAGAAGCAG AAAGCATCAA AACCTTTCAG TATAATGTGC AGATGGAGAA ACCTTTTACA 
GACGGGAATC TTACTGGTTT TATAAATTAT CAAATGATGG ATACTCATTC TGTTCAGCTT 
GAATTTTCTG TAAACTTATC GCTATTAGAT TTCATTAGAC CGTTAAAAAT CTCAACTGAA 
AACTTTGGCA AACTCTGGTT ATCCTTCGCA AATGATGTGA AGCAAAATGT AAAAGTGTCA 
GAATCTCAAG CTGCTCTGTC TTCTGCCCTA AAGACCCTGC AACAGAAACT AAGACTTCAT 
GTTGTTGAAA TTATAGGCAA CGAAGGGCTT TTGGCCTGTC AACTGCTCCC ATCCATCCCC 
TGTTTGCTGC ACTTTCGAGT TCATGGTGAT GTATTGGCCT TGTGGTTCAG ATCCTCCTGC 
TCTTCTCTTC CTGACTACTT ACTGTATCAG TGTCAAAAGG TGATGGAGGG ATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP4E1 ( XM_535479.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_535479.2 Canis lupus familiaris adaptor-related protein complex 4, epsilon 1 subunit (AP4E1), mRNA.

Price & Availability↑

CloneID	OCe28858
Clone ID Related Accession (Same CDS sequence)	XM_022412570.1 , XM_535479.5
Accession Version	XM_535479.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	AP-4 complex subunit epsilon-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535479.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACA TAGTGGAGAA GACGCTGACG GCGCTGCCGG GACTCTTTTT GCAGAACCAG 
TCGGTGGGCG GGCCCGCGGG CGTCAAGGCT TCTTTCTCTT CGCGGCTGGG CGGCCTCGTC 
CGCGGCATCA CAGCGCTAAC CTCCAAGCAC GAGGAAGAGA AACTAATCCA ACAGGAATTG 
AGTAATCTGA AAGCAACTGT TTCTGCTCCT ACTACAACAC TGAAAATGAT GAAGGAATGT 
ATGGTGAGAC TTATATATTG TGAAATGCTT GGATATGACG CTTCATTTGG CTATATACAT 
GCAATCAAGT TGGCACAACA AGGAAACCTT TTAGAAAAAA GAGTAGGTTA TTTGGCTGTT 
TCTTTATTTT TACATGAAAG TCATGAACTG CTGCTTCTCC TTGTGAATAC AGTTGTAAAG 
GATTTGCAGA GCACTAATCT GGTAGAAGTA TGTATGGCAC TTACTATTGT CAGCCAGATT 
TTCCCTCGAG AAATGATTCC AGCTGTTCTT CCATTAATTG AAGATAAACT TCAACATTCT 
AAGGAGATTA TACGAAGAAA AGCTGTTCTG GCATTATATA AATTCCATCT TATTGCTCCT 
AATCAAGTAC AGCATATCCA TATTAAGTTT CGGAAAGCAC TTTGTGACAG AGATGTTGGG 
GTCATGGCTG CTTCTTTGCA CATATACTTT AGGATGATTA AGGAGAATTC ATCTGGATAT 
AAAGACTTGA CGGGGAGTTT TGTGACAATT TTGAAGCAAG TAGTTGGAGG AAAGCTCCCA 
GTAGATTTCA ATTACCACAG TGTGCCAGCA CCATGGTTAC AAATCCAGCT CTTGAGAATA 
TTAGGACTAC TAGGAAAAGA TGATCAAAGG ACAAGTGAAT TAATGTATGA TGTTCTTGAT 
GAATCCTTAC GAAGAGCCGA GTTAAATCAC AATGTCACAT ATGCGATTCT GTTTGAATGT 
GTGCATACAG TTTATTCTAT TTATCCTAAA TCAGAATTAC TTGAGAAGGC TGCCAAGTGC 
ATTGGAAAAT TTGTTCTTTC ACCTAAAATA AATCTCAAGT ATTTAGGACT GAAGGCTCTT 
ACCTATGTTA TCCAGCAGGA TCCCACTTTG GCTCTTCAGC ACCAGATGAC AATAATTGAA 
TGCTTAGACC ATCCTGATCC CATTATTAAA AGAGAGACAC TGGAACTTCT TTACAGAATT 
ACTAATGCAC AGAATATAAC AGTGATTGTC CAGAAAATGC TTGAATATTT ACATCAGAGC 
AAAGAAGAAT ATATTATTGT CAATTTGGTT GGCAAAATAG CTGAGCTTGC TGAGAAATAT 
GCCCCTGATA ATGCATGGTT TATTCAGACC ATGAATGCTG TGTTTTCAGT GGGAGGAGAT 
GTCATGCATC CTGATATTCC CAATAACTTT TTGAGACTGC TGGCGGAAGG TTTTGATGAT 
GAAACAGAAG ACCAGCAATT AAGGCTTTAT GCAGTTCAGT CTTATCTCAC TTTACTAGAT 
GTGGAAAATG TGTTCTACCC ACAGAAATTT CTTCAAGTTA TGAGTTGGGT GTTAGGGGAA 
TATTCCTACC TCTTAGATAA AGACACACCA GAGGAAGTTC TAACCAAGCT CTACAAGTTA 
CTTATGAATG ACTCCGTTTC TTCAGAAACA AAAGCCTGGT TAATCGCAGC TGTTACCAAG 
CTGACGCCCC AAGCACACTC TTCTAACATA GTCGAGAGAT TAATCCAGGA ATTCACCATA 
TCTTTGGATA CTTGTATGAG ACAGCATGCA TTTGAATTGA AACACTTACG TGAGAATTTG 
GAACTTATGA AGAGTTTGCT TCCAGTTGAT AAAAGCTGTG AAGACATGGT GGTAGATGCT 
TCTTTATCTT TTCTGGATGG TTTTGTGGCT GAAGAACTTA GTCAGGGGGC AGCACCTTAC 
AAACCTCACC ACCAACGGCA GGAGGAAAAG CTTTCTCAGG AAAAAGTTCT CAATTTTGAA 
CCATATGGAC TCTCCTTTTC TTCATCTGGC TTCACTGGAC GACAGTCTCC TGCTGGCATT 
TCCCTTGGTT CAGATCTATC TGGGAATAGT GCAGAGACTG GACTGAAGGA GACAAATAGC 
TTGAAGCTGG AAGGTATAAA GAAATTGTGG GGGAAAGAGG GCTATCTTCC TAAGAAGGAA 
GGTAAAACTG GGGATGAAAC TGAAGCTCTG CCTGTTCCTC AAGAGAGCAT AATAATGGAG 
AATGTAGACC AAACTATAAC AAAAAAGGAT CAGTGTCAAG TCCTTACCCA ATCTAAAGAG 
GAGAAAGAAA AGCAGCTGCT GGCATCATCG TTATTTGTTG GGCTAGGATC AGAAAGTACA 
ATCAATTTGC TAGGAAAAGC AGATACTGTA TCTCACAAAT TCAGAAGGAA ATCAAAAGTC 
AAGGAAACCA AAAGTGGAGA AACAAGCAGT GCTCATAATA TCACCTGTTC TTACTTTAGT 
TCTTCGTCAA ATGCAACTTA TGAAGATGAT TATTATTTGG ATACTTTGCA GGATAGAGAA 
GACAAAGAAT TAAATAAATT TTCTCTCAAT TCTGAACTTT TGGATTCTGA GTCACTCACA 
GAACTACCTT CAGTTGAGAA ACTCTCCAAT TGCAAGCTGT CTTCACCATC TTTGTTTACA 
GATAACAACA TGGAAGGTTT TCACCCTTCT CAGCCCACTG CACCCTCAGT TGCTAATGAA 
ACCTCTTTAG CTTCTTCTTT ACTGGAAGAA ACTTCAGAGC ACATGCATTC AGATCTTGTG 
GAGGTCTGTC ATAATGAAAC CATATCAATG TCTTCTTACA AAATTTGGAA AGATGATTGT 
TTATTGATGG TATGGTCAGT TTCTAATAAG AGTGGTTTGG AATTGAAAAG TGCTAACTTA 
GAAATTGCTC CAGCAGAATA TTTCAAGATC ACTGAGCAAC CTGGATGCTG CTTGCCTGTA 
ATAGAAGCAG AAAGCATCAA AACCTTTCAG TATAATGTGC AGATGGAGAA ACCTTTTACA 
GACGGGAATC TTACTGGTTT TATAAATTAT CAAATGATGG ATACTCATTC TGTTCAGCTT 
GAATTTTCTG TAAACTTATC GCTATTAGAT TTCATTAGAC CGTTAAAAAT CTCAACTGAA 
AACTTTGGCA AACTCTGGTT ATCCTTCGCA AATGATGTGA AGCAAAATGT AAAAGTGTCA 
GAATCTCAAG CTGCTCTGTC TTCTGCCCTA AAGACCCTGC AACAGAAACT AAGACTTCAT 
GTTGTTGAAA TTATAGGCAA CGAAGGGCTT TTGGCCTGTC AACTGCTCCC ATCCATCCCC 
TGTTTGCTGC ACTTTCGAGT TCATGGTGAT GTATTGGCCT TGTGGTTCAG ATCCTCCTGC 
TCTTCTCTTC CTGACTACTT ACTGTATCAG TGTCAAAAGG TGATGGAGGG ATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535479.2
CDS	131..3547
Translation	MSDIVEKTLTALPGLFLQNQSVGGPAGVKASFSSRLGGLVRGITALTSKHEEEKLIQQELSNLKATVSAPTTTLKMMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTIVSQIFPREMIPAVLPLIEDKLQHSKEIIRRKAVLALYKFHLIAPNQVQHIHIKFRKALCDRDVGVMAASLHIYFRMIKENSSGYKDLTGSFVTILKQVVGGKLPVDFNYHSVPAPWLQIQLLRILGLLGKDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNITVIVQKMLEYLHQSKEEYIIVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLYAVQSYLTLLDVENVFYPQKFLQVMSWVLGEYSYLLDKDTPEEVLTKLYKLLMNDSVSSETKAWLIAAVTKLTPQAHSSNIVERLIQEFTISLDTCMRQHAFELKHLRENLELMKSLLPVDKSCEDMVVDASLSFLDGFVAEELSQGAAPYKPHHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDLSGNSAETGLKETNSLKLEGIKKLWGKEGYLPKKEGKTGDETEALPVPQESIIMENVDQTITKKDQCQVLTQSKEEKEKQLLASSLFVGLGSESTINLLGKADTVSHKFRRKSKVKETKSGETSSAHNITCSYFSSSSNATYEDDYYLDTLQDREDKELNKFSLNSELLDSESLTELPSVEKLSNCKLSSPSLFTDNNMEGFHPSQPTAPSVANETSLASSLLEETSEHMHSDLVEVCHNETISMSSYKIWKDDCLLMVWSVSNKSGLELKSANLEIAPAEYFKITEQPGCCLPVIEAESIKTFQYNVQMEKPFTDGNLTGFINYQMMDTHSVQLEFSVNLSLLDFIRPLKISTENFGKLWLSFANDVKQNVKVSESQAALSSALKTLQQKLRLHVVEIIGNEGLLACQLLPSIPCLLHFRVHGDVLALWFRSSCSSLPDYLLYQCQKVMEGS

Target ORF information:

RefSeq Version	XM_535479.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adaptor-related protein complex 4, epsilon 1 subunit (AP4E1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535479.5

ORF Insert Sequence:

ATGAGCGACA TAGTGGAGAA GACGCTGACG GCGCTGCCGG GACTCTTTTT GCAGAACCAG 
TCGGTGGGCG GGCCCGCGGG CGTCAAGGCT TCTTTCTCTT CGCGGCTGGG CGGCCTCGTC 
CGCGGCATCA CAGCGCTAAC CTCCAAGCAC GAGGAAGAGA AACTAATCCA ACAGGAATTG 
AGTAATCTGA AAGCAACTGT TTCTGCTCCT ACTACAACAC TGAAAATGAT GAAGGAATGT 
ATGGTGAGAC TTATATATTG TGAAATGCTT GGATATGACG CTTCATTTGG CTATATACAT 
GCAATCAAGT TGGCACAACA AGGAAACCTT TTAGAAAAAA GAGTAGGTTA TTTGGCTGTT 
TCTTTATTTT TACATGAAAG TCATGAACTG CTGCTTCTCC TTGTGAATAC AGTTGTAAAG 
GATTTGCAGA GCACTAATCT GGTAGAAGTA TGTATGGCAC TTACTATTGT CAGCCAGATT 
TTCCCTCGAG AAATGATTCC AGCTGTTCTT CCATTAATTG AAGATAAACT TCAACATTCT 
AAGGAGATTA TACGAAGAAA AGCTGTTCTG GCATTATATA AATTCCATCT TATTGCTCCT 
AATCAAGTAC AGCATATCCA TATTAAGTTT CGGAAAGCAC TTTGTGACAG AGATGTTGGG 
GTCATGGCTG CTTCTTTGCA CATATACTTT AGGATGATTA AGGAGAATTC ATCTGGATAT 
AAAGACTTGA CGGGGAGTTT TGTGACAATT TTGAAGCAAG TAGTTGGAGG AAAGCTCCCA 
GTAGATTTCA ATTACCACAG TGTGCCAGCA CCATGGTTAC AAATCCAGCT CTTGAGAATA 
TTAGGACTAC TAGGAAAAGA TGATCAAAGG ACAAGTGAAT TAATGTATGA TGTTCTTGAT 
GAATCCTTAC GAAGAGCCGA GTTAAATCAC AATGTCACAT ATGCGATTCT GTTTGAATGT 
GTGCATACAG TTTATTCTAT TTATCCTAAA TCAGAATTAC TTGAGAAGGC TGCCAAGTGC 
ATTGGAAAAT TTGTTCTTTC ACCTAAAATA AATCTCAAGT ATTTAGGACT GAAGGCTCTT 
ACCTATGTTA TCCAGCAGGA TCCCACTTTG GCTCTTCAGC ACCAGATGAC AATAATTGAA 
TGCTTAGACC ATCCTGATCC CATTATTAAA AGAGAGACAC TGGAACTTCT TTACAGAATT 
ACTAATGCAC AGAATATAAC AGTGATTGTC CAGAAAATGC TTGAATATTT ACATCAGAGC 
AAAGAAGAAT ATATTATTGT CAATTTGGTT GGCAAAATAG CTGAGCTTGC TGAGAAATAT 
GCCCCTGATA ATGCATGGTT TATTCAGACC ATGAATGCTG TGTTTTCAGT GGGAGGAGAT 
GTCATGCATC CTGATATTCC CAATAACTTT TTGAGACTGC TGGCGGAAGG TTTTGATGAT 
GAAACAGAAG ACCAGCAATT AAGGCTTTAT GCAGTTCAGT CTTATCTCAC TTTACTAGAT 
GTGGAAAATG TGTTCTACCC ACAGAAATTT CTTCAAGTTA TGAGTTGGGT GTTAGGGGAA 
TATTCCTACC TCTTAGATAA AGACACACCA GAGGAAGTTC TAACCAAGCT CTACAAGTTA 
CTTATGAATG ACTCCGTTTC TTCAGAAACA AAAGCCTGGT TAATCGCAGC TGTTACCAAG 
CTGACGCCCC AAGCACACTC TTCTAACATA GTCGAGAGAT TAATCCAGGA ATTCACCATA 
TCTTTGGATA CTTGTATGAG ACAGCATGCA TTTGAATTGA AACACTTACG TGAGAATTTG 
GAACTTATGA AGAGTTTGCT TCCAGTTGAT AAAAGCTGTG AAGACATGGT GGTAGATGCT 
TCTTTATCTT TTCTGGATGG TTTTGTGGCT GAAGAACTTA GTCAGGGGGC AGCACCTTAC 
AAACCTCACC ACCAACGGCA GGAGGAAAAG CTTTCTCAGG AAAAAGTTCT CAATTTTGAA 
CCATATGGAC TCTCCTTTTC TTCATCTGGC TTCACTGGAC GACAGTCTCC TGCTGGCATT 
TCCCTTGGTT CAGATCTATC TGGGAATAGT GCAGAGACTG GACTGAAGGA GACAAATAGC 
TTGAAGCTGG AAGGTATAAA GAAATTGTGG GGGAAAGAGG GCTATCTTCC TAAGAAGGAA 
GGTAAAACTG GGGATGAAAC TGAAGCTCTG CCTGTTCCTC AAGAGAGCAT AATAATGGAG 
AATGTAGACC AAACTATAAC AAAAAAGGAT CAGTGTCAAG TCCTTACCCA ATCTAAAGAG 
GAGAAAGAAA AGCAGCTGCT GGCATCATCG TTATTTGTTG GGCTAGGATC AGAAAGTACA 
ATCAATTTGC TAGGAAAAGC AGATACTGTA TCTCACAAAT TCAGAAGGAA ATCAAAAGTC 
AAGGAAACCA AAAGTGGAGA AACAAGCAGT GCTCATAATA TCACCTGTTC TTACTTTAGT 
TCTTCGTCAA ATGCAACTTA TGAAGATGAT TATTATTTGG ATACTTTGCA GGATAGAGAA 
GACAAAGAAT TAAATAAATT TTCTCTCAAT TCTGAACTTT TGGATTCTGA GTCACTCACA 
GAACTACCTT CAGTTGAGAA ACTCTCCAAT TGCAAGCTGT CTTCACCATC TTTGTTTACA 
GATAACAACA TGGAAGGTTT TCACCCTTCT CAGCCCACTG CACCCTCAGT TGCTAATGAA 
ACCTCTTTAG CTTCTTCTTT ACTGGAAGAA ACTTCAGAGC ACATGCATTC AGATCTTGTG 
GAGGTCTGTC ATAATGAAAC CATATCAATG TCTTCTTACA AAATTTGGAA AGATGATTGT 
TTATTGATGG TATGGTCAGT TTCTAATAAG AGTGGTTTGG AATTGAAAAG TGCTAACTTA 
GAAATTGCTC CAGCAGAATA TTTCAAGATC ACTGAGCAAC CTGGATGCTG CTTGCCTGTA 
ATAGAAGCAG AAAGCATCAA AACCTTTCAG TATAATGTGC AGATGGAGAA ACCTTTTACA 
GACGGGAATC TTACTGGTTT TATAAATTAT CAAATGATGG ATACTCATTC TGTTCAGCTT 
GAATTTTCTG TAAACTTATC GCTATTAGAT TTCATTAGAC CGTTAAAAAT CTCAACTGAA 
AACTTTGGCA AACTCTGGTT ATCCTTCGCA AATGATGTGA AGCAAAATGT AAAAGTGTCA 
GAATCTCAAG CTGCTCTGTC TTCTGCCCTA AAGACCCTGC AACAGAAACT AAGACTTCAT 
GTTGTTGAAA TTATAGGCAA CGAAGGGCTT TTGGCCTGTC AACTGCTCCC ATCCATCCCC 
TGTTTGCTGC ACTTTCGAGT TCATGGTGAT GTATTGGCCT TGTGGTTCAG ATCCTCCTGC 
TCTTCTCTTC CTGACTACTT ACTGTATCAG TGTCAAAAGG TGATGGAGGG ATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP4E1 ( XM_022412571.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022268279.1 Canis lupus familiaris adaptor related protein complex 4 epsilon 1 subunit (AP4E1), transcript variant X2, mRNA.

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CloneID	OCe28860
Clone ID Related Accession (Same CDS sequence)	XM_022412571.1
Accession Version	XM_022412571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	AP-4 complex subunit epsilon-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGG AATGTATGGT GAGACTTATA TATTGTGAAA TGCTTGGATA TGACGCTTCA 
TTTGGCTATA TACATGCAAT CAAGTTGGCA CAACAAGGAA ACCTTTTAGA AAAAAGAGTA 
GGTTATTTGG CTGTTTCTTT ATTTTTACAT GAAAGTCATG AACTGCTGCT TCTCCTTGTG 
AATACAGTTG TAAAGGATTT GCAGAGCACT AATCTGGTAG AAGTATGTAT GGCACTTACT 
ATTGTCAGCC AGATTTTCCC TCGAGAAATG ATTCCAGCTG TTCTTCCATT AATTGAAGAT 
AAACTTCAAC ATTCTAAGGA GATTATACGA AGAAAAGCTG TTCTGGCATT ATATAAATTC 
CATCTTATTG CTCCTAATCA AGTACAGCAT ATCCATATTA AGTTTCGGAA AGCACTTTGT 
GACAGAGATG TTGGGGTCAT GGCTGCTTCT TTGCACATAT ACTTTAGGAT GATTAAGGAG 
AATTCATCTG GATATAAAGA CTTGACGGGG AGTTTTGTGA CAATTTTGAA GCAAGTAGTT 
GGAGGAAAGC TCCCAGTAGA TTTCAATTAC CACAGTGTGC CAGCACCATG GTTACAAATC 
CAGCTCTTGA GAATATTAGG ACTACTAGGA AAAGATGATC AAAGGACAAG TGAATTAATG 
TATGATGTTC TTGATGAATC CTTACGAAGA GCCGAGTTAA ATCACAATGT CACATATGCG 
ATTCTGTTTG AATGTGTGCA TACAGTTTAT TCTATTTATC CTAAATCAGA ATTACTTGAG 
AAGGCTGCCA AGTGCATTGG AAAATTTGTT CTTTCACCTA AAATAAATCT CAAGTATTTA 
GGACTGAAGG CTCTTACCTA TGTTATCCAG CAGGATCCCA CTTTGGCTCT TCAGCACCAG 
ATGACAATAA TTGAATGCTT AGACCATCCT GATCCCATTA TTAAAAGAGA GACACTGGAA 
CTTCTTTACA GAATTACTAA TGCACAGAAT ATAACAGTGA TTGTCCAGAA AATGCTTGAA 
TATTTACATC AGAGCAAAGA AGAATATATT ATTGTCAATT TGGTTGGCAA AATAGCTGAG 
CTTGCTGAGA AATATGCCCC TGATAATGCA TGGTTTATTC AGACCATGAA TGCTGTGTTT 
TCAGTGGGAG GAGATGTCAT GCATCCTGAT ATTCCCAATA ACTTTTTGAG ACTGCTGGCG 
GAAGGTTTTG ATGATGAAAC AGAAGACCAG CAATTAAGGC TTTATGCAGT TCAGTCTTAT 
CTCACTTTAC TAGATGTGGA AAATGTGTTC TACCCACAGA AATTTCTTCA AGTTATGAGT 
TGGGTGTTAG GGGAATATTC CTACCTCTTA GATAAAGACA CACCAGAGGA AGTTCTAACC 
AAGCTCTACA AGTTACTTAT GAATGACTCC GTTTCTTCAG AAACAAAAGC CTGGTTAATC 
GCAGCTGTTA CCAAGCTGAC GCCCCAAGCA CACTCTTCTA ACATAGTCGA GAGATTAATC 
CAGGAATTCA CCATATCTTT GGATACTTGT ATGAGACAGC ATGCATTTGA ATTGAAACAC 
TTACGTGAGA ATTTGGAACT TATGAAGAGT TTGCTTCCAG TTGATAAAAG CTGTGAAGAC 
ATGGTGGTAG ATGCTTCTTT ATCTTTTCTG GATGGTTTTG TGGCTGAAGA ACTTAGTCAG 
GGGGCAGCAC CTTACAAACC TCACCACCAA CGGCAGGAGG AAAAGCTTTC TCAGGAAAAA 
GTTCTCAATT TTGAACCATA TGGACTCTCC TTTTCTTCAT CTGGCTTCAC TGGACGACAG 
TCTCCTGCTG GCATTTCCCT TGGTTCAGAT CTATCTGGGA ATAGTGCAGA GACTGGACTG 
AAGGAGACAA ATAGCTTGAA GCTGGAAGGT ATAAAGAAAT TGTGGGGGAA AGAGGGCTAT 
CTTCCTAAGA AGGAAGGTAA AACTGGGGAT GAAACTGAAG CTCTGCCTGT TCCTCAAGAG 
AGCATAATAA TGGAGAATGT AGACCAAACT ATAACAAAAA AGGATCAGTG TCAAGTCCTT 
ACCCAATCTA AAGAGGAGAA AGAAAAGCAG CTGCTGGCAT CATCGTTATT TGTTGGGCTA 
GGATCAGAAA GTACAATCAA TTTGCTAGGA AAAGCAGATA CTGTATCTCA CAAATTCAGA 
AGGAAATCAA AAGTCAAGGA AACCAAAAGT GGAGAAACAA GCAGTGCTCA TAATATCACC 
TGTTCTTACT TTAGTTCTTC GTCAAATGCA ACTTATGAAG ATGATTATTA TTTGGATACT 
TTGCAGGATA GAGAAGACAA AGAATTAAAT AAATTTTCTC TCAATTCTGA ACTTTTGGAT 
TCTGAGTCAC TCACAGAACT ACCTTCAGTT GAGAAACTCT CCAATTGCAA GCTGTCTTCA 
CCATCTTTGT TTACAGATAA CAACATGGAA GGTTTTCACC CTTCTCAGCC CACTGCACCC 
TCAGTTGCTA ATGAAACCTC TTTAGCTTCT TCTTTACTGG AAGAAACTTC AGAGCACATG 
CATTCAGATC TTGTGGAGGT CTGTCATAAT GAAACCATAT CAATGTCTTC TTACAAAATT 
TGGAAAGATG ATTGTTTATT GATGGTATGG TCAGTTTCTA ATAAGAGTGG TTTGGAATTG 
AAAAGTGCTA ACTTAGAAAT TGCTCCAGCA GAATATTTCA AGATCACTGA GCAACCTGGA 
TGCTGCTTGC CTGTAATAGA AGCAGAAAGC ATCAAAACCT TTCAGTATAA TGTGCAGATG 
GAGAAACCTT TTACAGACGG GAATCTTACT GGTTTTATAA ATTATCAAAT GATGGATACT 
CATTCTGTTC AGCTTGAATT TTCTGTAAAC TTATCGCTAT TAGATTTCAT TAGACCGTTA 
AAAATCTCAA CTGAAAACTT TGGCAAACTC TGGTTATCCT TCGCAAATGA TGTGAAGCAA 
AATGTAAAAG TGTCAGAATC TCAAGCTGCT CTGTCTTCTG CCCTAAAGAC CCTGCAACAG 
AAACTAAGAC TTCATGTTGT TGAAATTATA GGCAACGAAG GGCTTTTGGC CTGTCAACTG 
CTCCCATCCA TCCCCTGTTT GCTGCACTTT CGAGTTCATG GTGATGTATT GGCCTTGTGG 
TTCAGATCCT CCTGCTCTTC TCTTCCTGAC TACTTACTGT ATCAGTGTCA AAAGGTGATG 
GAGGGATCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268279.1
CDS	227..3418
Translation	MMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTIVSQIFPREMIPAVLPLIEDKLQHSKEIIRRKAVLALYKFHLIAPNQVQHIHIKFRKALCDRDVGVMAASLHIYFRMIKENSSGYKDLTGSFVTILKQVVGGKLPVDFNYHSVPAPWLQIQLLRILGLLGKDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNITVIVQKMLEYLHQSKEEYIIVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLYAVQSYLTLLDVENVFYPQKFLQVMSWVLGEYSYLLDKDTPEEVLTKLYKLLMNDSVSSETKAWLIAAVTKLTPQAHSSNIVERLIQEFTISLDTCMRQHAFELKHLRENLELMKSLLPVDKSCEDMVVDASLSFLDGFVAEELSQGAAPYKPHHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDLSGNSAETGLKETNSLKLEGIKKLWGKEGYLPKKEGKTGDETEALPVPQESIIMENVDQTITKKDQCQVLTQSKEEKEKQLLASSLFVGLGSESTINLLGKADTVSHKFRRKSKVKETKSGETSSAHNITCSYFSSSSNATYEDDYYLDTLQDREDKELNKFSLNSELLDSESLTELPSVEKLSNCKLSSPSLFTDNNMEGFHPSQPTAPSVANETSLASSLLEETSEHMHSDLVEVCHNETISMSSYKIWKDDCLLMVWSVSNKSGLELKSANLEIAPAEYFKITEQPGCCLPVIEAESIKTFQYNVQMEKPFTDGNLTGFINYQMMDTHSVQLEFSVNLSLLDFIRPLKISTENFGKLWLSFANDVKQNVKVSESQAALSSALKTLQQKLRLHVVEIIGNEGLLACQLLPSIPCLLHFRVHGDVLALWFRSSCSSLPDYLLYQCQKVMEGS

Target ORF information:

RefSeq Version	XM_022412571.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adaptor related protein complex 4 epsilon 1 subunit (AP4E1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412571.1

ORF Insert Sequence:

ATGATGAAGG AATGTATGGT GAGACTTATA TATTGTGAAA TGCTTGGATA TGACGCTTCA 
TTTGGCTATA TACATGCAAT CAAGTTGGCA CAACAAGGAA ACCTTTTAGA AAAAAGAGTA 
GGTTATTTGG CTGTTTCTTT ATTTTTACAT GAAAGTCATG AACTGCTGCT TCTCCTTGTG 
AATACAGTTG TAAAGGATTT GCAGAGCACT AATCTGGTAG AAGTATGTAT GGCACTTACT 
ATTGTCAGCC AGATTTTCCC TCGAGAAATG ATTCCAGCTG TTCTTCCATT AATTGAAGAT 
AAACTTCAAC ATTCTAAGGA GATTATACGA AGAAAAGCTG TTCTGGCATT ATATAAATTC 
CATCTTATTG CTCCTAATCA AGTACAGCAT ATCCATATTA AGTTTCGGAA AGCACTTTGT 
GACAGAGATG TTGGGGTCAT GGCTGCTTCT TTGCACATAT ACTTTAGGAT GATTAAGGAG 
AATTCATCTG GATATAAAGA CTTGACGGGG AGTTTTGTGA CAATTTTGAA GCAAGTAGTT 
GGAGGAAAGC TCCCAGTAGA TTTCAATTAC CACAGTGTGC CAGCACCATG GTTACAAATC 
CAGCTCTTGA GAATATTAGG ACTACTAGGA AAAGATGATC AAAGGACAAG TGAATTAATG 
TATGATGTTC TTGATGAATC CTTACGAAGA GCCGAGTTAA ATCACAATGT CACATATGCG 
ATTCTGTTTG AATGTGTGCA TACAGTTTAT TCTATTTATC CTAAATCAGA ATTACTTGAG 
AAGGCTGCCA AGTGCATTGG AAAATTTGTT CTTTCACCTA AAATAAATCT CAAGTATTTA 
GGACTGAAGG CTCTTACCTA TGTTATCCAG CAGGATCCCA CTTTGGCTCT TCAGCACCAG 
ATGACAATAA TTGAATGCTT AGACCATCCT GATCCCATTA TTAAAAGAGA GACACTGGAA 
CTTCTTTACA GAATTACTAA TGCACAGAAT ATAACAGTGA TTGTCCAGAA AATGCTTGAA 
TATTTACATC AGAGCAAAGA AGAATATATT ATTGTCAATT TGGTTGGCAA AATAGCTGAG 
CTTGCTGAGA AATATGCCCC TGATAATGCA TGGTTTATTC AGACCATGAA TGCTGTGTTT 
TCAGTGGGAG GAGATGTCAT GCATCCTGAT ATTCCCAATA ACTTTTTGAG ACTGCTGGCG 
GAAGGTTTTG ATGATGAAAC AGAAGACCAG CAATTAAGGC TTTATGCAGT TCAGTCTTAT 
CTCACTTTAC TAGATGTGGA AAATGTGTTC TACCCACAGA AATTTCTTCA AGTTATGAGT 
TGGGTGTTAG GGGAATATTC CTACCTCTTA GATAAAGACA CACCAGAGGA AGTTCTAACC 
AAGCTCTACA AGTTACTTAT GAATGACTCC GTTTCTTCAG AAACAAAAGC CTGGTTAATC 
GCAGCTGTTA CCAAGCTGAC GCCCCAAGCA CACTCTTCTA ACATAGTCGA GAGATTAATC 
CAGGAATTCA CCATATCTTT GGATACTTGT ATGAGACAGC ATGCATTTGA ATTGAAACAC 
TTACGTGAGA ATTTGGAACT TATGAAGAGT TTGCTTCCAG TTGATAAAAG CTGTGAAGAC 
ATGGTGGTAG ATGCTTCTTT ATCTTTTCTG GATGGTTTTG TGGCTGAAGA ACTTAGTCAG 
GGGGCAGCAC CTTACAAACC TCACCACCAA CGGCAGGAGG AAAAGCTTTC TCAGGAAAAA 
GTTCTCAATT TTGAACCATA TGGACTCTCC TTTTCTTCAT CTGGCTTCAC TGGACGACAG 
TCTCCTGCTG GCATTTCCCT TGGTTCAGAT CTATCTGGGA ATAGTGCAGA GACTGGACTG 
AAGGAGACAA ATAGCTTGAA GCTGGAAGGT ATAAAGAAAT TGTGGGGGAA AGAGGGCTAT 
CTTCCTAAGA AGGAAGGTAA AACTGGGGAT GAAACTGAAG CTCTGCCTGT TCCTCAAGAG 
AGCATAATAA TGGAGAATGT AGACCAAACT ATAACAAAAA AGGATCAGTG TCAAGTCCTT 
ACCCAATCTA AAGAGGAGAA AGAAAAGCAG CTGCTGGCAT CATCGTTATT TGTTGGGCTA 
GGATCAGAAA GTACAATCAA TTTGCTAGGA AAAGCAGATA CTGTATCTCA CAAATTCAGA 
AGGAAATCAA AAGTCAAGGA AACCAAAAGT GGAGAAACAA GCAGTGCTCA TAATATCACC 
TGTTCTTACT TTAGTTCTTC GTCAAATGCA ACTTATGAAG ATGATTATTA TTTGGATACT 
TTGCAGGATA GAGAAGACAA AGAATTAAAT AAATTTTCTC TCAATTCTGA ACTTTTGGAT 
TCTGAGTCAC TCACAGAACT ACCTTCAGTT GAGAAACTCT CCAATTGCAA GCTGTCTTCA 
CCATCTTTGT TTACAGATAA CAACATGGAA GGTTTTCACC CTTCTCAGCC CACTGCACCC 
TCAGTTGCTA ATGAAACCTC TTTAGCTTCT TCTTTACTGG AAGAAACTTC AGAGCACATG 
CATTCAGATC TTGTGGAGGT CTGTCATAAT GAAACCATAT CAATGTCTTC TTACAAAATT 
TGGAAAGATG ATTGTTTATT GATGGTATGG TCAGTTTCTA ATAAGAGTGG TTTGGAATTG 
AAAAGTGCTA ACTTAGAAAT TGCTCCAGCA GAATATTTCA AGATCACTGA GCAACCTGGA 
TGCTGCTTGC CTGTAATAGA AGCAGAAAGC ATCAAAACCT TTCAGTATAA TGTGCAGATG 
GAGAAACCTT TTACAGACGG GAATCTTACT GGTTTTATAA ATTATCAAAT GATGGATACT 
CATTCTGTTC AGCTTGAATT TTCTGTAAAC TTATCGCTAT TAGATTTCAT TAGACCGTTA 
AAAATCTCAA CTGAAAACTT TGGCAAACTC TGGTTATCCT TCGCAAATGA TGTGAAGCAA 
AATGTAAAAG TGTCAGAATC TCAAGCTGCT CTGTCTTCTG CCCTAAAGAC CCTGCAACAG 
AAACTAAGAC TTCATGTTGT TGAAATTATA GGCAACGAAG GGCTTTTGGC CTGTCAACTG 
CTCCCATCCA TCCCCTGTTT GCTGCACTTT CGAGTTCATG GTGATGTATT GGCCTTGTGG 
TTCAGATCCT CCTGCTCTTC TCTTCCTGAC TACTTACTGT ATCAGTGTCA AAAGGTGATG 
GAGGGATCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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AP4E1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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