ORF » Species Summary » Xenopus tropicalis » trio cDNA ORF clone

trio cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following trio gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trio cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01813 NM_001129921.1
Latest version!		 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32253 XM_012964353.3
Latest version!		 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32254 XM_012964354.3
Latest version!		 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32255 XM_012964355.3
Latest version!		 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32256 XM_012964356.2
Latest version!		 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa44862 XM_018094560.1
Latest version!		 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa59535 XM_031903241.1
Latest version!		 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OXa32253 XM_012964353.2 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32255 XM_012964355.2 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa32254 XM_012964354.2 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    trio ( NM_001129921.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123393.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), mRNA.
    Price & Availability↑
    CloneID OXa01813
    Clone ID Related Accession (Same CDS sequence) NM_001129921.1
    Accession Version NM_001129921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576857600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167460.1. ##Evidence-Data-START## Transcript exon combination :: BC167460.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028309, SAMD00028310 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGCAGACTC ATCGCTGGAA AGATGACGGA GAAACAACAT TAGGTTTTCG TAGAAATGAT 
    GAATTGAAAG CAATGGAGGT GTTGCCAATT TTAAAAGAAA AAGTGGCATA TCTATCAGGT 
    GGCAGAGATA AACGAGGTGG TCCTGTATTA ACATTTCCAG CACGCAGCAA CCATGATCGA 
    ATACGACAAG AGGATCTCCG AAGACTGATT TCATATCTTG CTTGTATTCC TAGTGAGGAG 
    GTGTGCAAGC GAGGCTTCAC AGTGATTGTA GATATGCGTG GTTCCAAGTG GGATTCTATT 
    AAACCCTTGC TGAAAATTTT ACAAGAATCC TTTCCATGCT GTATTCATGT TGCACTAATT 
    ATCAAACCAG ATAACTTCTG GCAGAAGCAA AGAACTAATT TTGGAAGTTC TAAGTTTGAA 
    TTTGAGACAA GTATGGTGTC TTTAGAAGGC CTTACCAAAG TAGTTGACCC TTCCCAGCTA 
    ACTCCAGAAT TTGAGGGTTG CTTGGAATAC AACCATGAAG AGTGGATAGA GATCAGAGTA 
    GCATTTGAGG ATTTTATTAA TAATGCAACT CATATGCTGT CTCGATTAGA GGAATTACAG 
    GAGATGCTTG CAATTAAAGA GTTGCCTCAG GATTTAGAGG GGGCACGCAA TATGATTGAA 
    GAACATTCTC AAATGAAAAA AAGGGTTATA AAGGCTCCTA TTGAAGATCT TGATTTAGAA 
    GGGCAAAAGC TTTTACAAAC AATACAAAGC AGTGATAGCT TTCCTAAGAA GAACTCTAGT 
    TCTGGAAATG CAGACCTTCA AAATCTTCTT CCCAAAGTTT CCACAATGTT AGACAGGCTT 
    CATTCAACAC GACAACATCT TCATCAGATG TGGCACATGC GAAAAGTAAA ACTGGACCAG 
    TGCTTTCAGC TAAGGTTGTT TGAGCAGGAT GCTGAGAAGA TGTTTGACTG GATTACACAC 
    AACAAAGGTC TGTTTCTGAC AAGTTATACT GACATTGGCA CCCGTAATAA TCATGCTATG 
    GAACTTCAGA CACAGCACAA TCATTTTGCT ATGAACTGCA TGAATGTCTA TGTAAACATT 
    AGCCGAATAA TGTCTGTGGC AAATCGACTA GTAGAATCTG GACACTATGC CTCACAGCAA 
    ATTAAGCAGA TAGCTAACCA GTTGGAGCAA GAGTGGAAGG CTTTTGCCGC AGCCCTTGAT 
    GAACGTAGTA CGTTGCTTGA CATGTCTGCA ATGTTCCATC AAAAAGCGGA GCAGTACATG 
    GGCAATGTGG ATTCATGGTG CAAAGCTTGT GGGGAAGTGG ATCTTCCATC AGAGCTGCAG 
    GATCTAGAAG ATGCCATTCA TCACCATCAG GGAATATATG AGCATATCAC TTTAGCATAT 
    TCAGAGGTTA GCCAGGATGG TAAATCTTTA TTAGACAAAC TCCAGAGACC ATTGACTCCT 
    GGTAGTTCTG ACTCACTGAC AGCTTCTGCC AACTATTCAA AGGCTGTGCA TCACGTCTTA 
    GATGCAATAC ATGAAGTCTT GCACCACCAG CGTCAGTTGG AAAACATCTG GCAACATCGC 
    AAAGTTCGTC TGCATCAACG CCTACAGCTC TGTGTTTTTC AGCAAGATGT TCAGCAGGTC 
    TTGGATTGGA TAGAGAACCA TGGTGAAGCC TTTCTCAGCA AACACACTGG GGTAGGCAAG 
    TCTCTGCACA GGGCCAGAGC CTTGCAAAAG CGACATGAAG ACTTTGAAGA AGTCGCTCAG 
    AATACATACA CCAATGCTGA TAAGCTGCTG GAAGCTGCAG AACAGTTGGC CCAGACAGGA 
    GAGTGCGATC CAGAGGAAAT ATACCAGGCT GCTCATCAGC TTGAGGATCG GATTCAGGAT 
    TTTGTAAGAC GTGTAGAACA AAGAAAAGTT CTCTTGGACA TGTCTGTATC ATTCCACTCG 
    CATGTTAAAG AGCTATGGAC ATGGCTGGAG GACCTACAAA AGGAACTGCT GGATGATGTC 
    TATGCGGAGT CAGTAGAAGC TGTACAGGAC TTGATCAAAC GGTTTGGACA ACAGCAGCAG 
    ACAACCCTCC AGTTTACAGT AAATGTCATC AAAGAAGGGG AGGATCTAAT TCAGCAACTA 
    AGAGATTCTG CTATATCCAG CAACAAAACT CCTCACAACA GCTCAATCAA TCACATTGAG 
    ACTGTCCTTC AACAACTTGA TGAGGCCCAA TCACAGATGG AGGAGCTTTT CCAAGAGAGA 
    AAAATCAAAC TTGAGCTATT CTTGCAGCTT CGCATCTTTG AGAGAGATGC CATTGATATC 
    ATCTCAGACT TGGAATCATG GAATGATGAA CTTTCTCAGC AAATGAATGA TTTTGACACA 
    GAGGACCTCA CACTAGCCGA GCAAAGGCTA CAGCATCACG CAGACAAAGC TCTGACAATG 
    AACAACCTGA CATTTGATGT CATCCACCAA GGACAAGAGT TACTACAATA TGTGAATGAA 
    GTTCAGAACT CAGGGGTTGA GCTACTCTGT GATAGAGATG TAGATATGGC TACTAGAGTT 
    CAGGACCTGT TGGAATTCCT CCATGAGAAA CAACAGGAGT TGGATCTAGC AGCTGAGCAG 
    CACCGAAAAC ATCTAGAACA ATGTGTGCAA CTACGACATT TGCAAGCTGA AGTCAAACAG 
    GTACTCGGCT GGATACGGAA TGGAGAGTCT ATGTTAAATG CCGGGCTTAT CACTGCCAGT 
    TCATTGCAAG AAGCAGAGCA ACTTCAAAGG GAACATGAGC AGTTCCAGCA TGCAATAGAA 
    AAAACCCACC AGAGTGCTCT GCAGGTGCAG CAGAAGGCAG AGGCCATGCT TCAAGCAAAC 
    CATTACGACA TGGATATGAT CCGGGACTGT GCTGAAAAAG TGGCCTCTCA TTGGCAACAG 
    TTGATGCTTA AAATGGAAGA CCGTCTTAAG CTTGTCAATG CCTCTGTGGC CTTTTATAAA 
    ACCTCAGAGC AGGTATGCAG TGTTCTAGAG AGCCTAGAGC AGGAGTACAA GCGGGAGGAA 
    GATTGGTGTG GCGGAGCTGA TAAACTTGGG CCTAACTCAG AGACTGACCA TGTTACTCCT 
    ATGATCAGCA AACACCTTGA ACAAAAGGAA GCCTTTCTTA AGGCCTGCAC TTTGGCTCGC 
    AGAAATGCTG ATGTCTTTCT GAAATACTTG CACAGGAATA GTGTCAATAT GCCTGGAATG 
    GTGACACATG TAAAGGCTCC TGAACAGCAA GTAAAAAATA TTTTAAATGA GCTGTTTCAG 
    AGGGAAAATA GAGTTCTTCA TTATTGGACC TTGCGGAAAA GGAGACTAGA CCATTGCCAG 
    CAGTATGTAG TATTTGAAAG AAGTGCCAAA CAGGCTCTGG AGTGGATCCA AGATAATGGA 
    GAATTTTACA TTTCCACACA CACCTCCACT GGCTCCACAA TTCAGCATAC ACAAGAACTC 
    TTAAAAGAGC ATGAGGAGTT TCAGATCACA GCAAAGCAAA CCAAAGAGAG GGTAAAGCTG 
    TTGATACAGC TGGCTGATGG CTTTTGTGAA AAAGGGCATG CTCATGCTGC AGAAATTAAA 
    AAATGTGTTA CAGCGGTGGA CAAGAGGTAC AGAGACTTCT CGCTGCGTAT GGAAAAGTAC 
    AGAACCAGCT TAGAGAAGGC TTTGGGCATT TCTTCTGATT CCAAGTCGAG TAAAAGCCTT 
    CAGTTGGATA TTATTCCAGC CACTGCCCCT GGTTCCGAAG TCAAGCTTCG TGATGCAACA 
    CATGAACTCA ATGAGGAGAA AAGAAAATCT GCTCGTCGAA AAGAATTCAT CATGGCAGAA 
    CTTATACAAA CTGAAAAAGC ATATGTTCGT GATCTTCGGG AATGCATGGA TACATATCTG 
    TGGGAAATGA CAAGCGGAGT TGAAGAGATC CCACCAGGCA TTGTAAATAA AGAACACATT 
    ATCTTTGGAA ACATGCAGGA AATATATGAA TTTCATAACA ACATATTCTT AAAGGAGCTT 
    GAGAAGTATG AGCAGCTACC TGAAGATGTT GGACATTGTT TTGTCACATG GGCTGACAAA 
    TTTCAGATGT ATGTGTCCTA CTGTAAGAAT AAGCCTGATT CAACACAACT TATCTTGGAA 
    CAAGCAGGAA CATATTTTGA TGAAATTCAG CAGCGCCATG AGCTGGTCAA CTCCATCTCT 
    TCTTATCTTA TCAAACCTGT GCAAAGGATA ACAAAATATC AGCTTCTTTT AAAAGAGCTG 
    CTGACATGCT GCGAAGAAGG TAAAGGAGAG ATTAAAGATG GGCTGGAAGT GATGCTAAGT 
    GTACCAAAAA GAGCCAACGA TGCAATGCAT CTCAGCATGC TTGAAGGGTT TGATGAGAAC 
    ATTGAATCTC AGGGAGAACT TATTCTGCAA GAGTCTTTTC AAGTGTGGGA TCCTAAAACC 
    TTAATAAGAA AGGGGCGTGA GAGACATCTC TTCTTGTTTG AAATGTCTTT GGTCTTCAGC 
    AAAGAAGTGA AAGATTCAAG TGGAAGGAGC AAATATATTT ACAAATCAAA ATTATTTACC 
    TCAGAGCTTG GAGTCACAGA ACATGTAGAA GGTGATCCTT GCAAATTTGC ACTTTGGGTA 
    GGCAGAACGC CAACATCTGA TAACAAAATA GTCCTTAAGG GCTCAAGTAT TGAGAACAAG 
    CAAGATTGGA TCAAGCATAT AAGAGAAGTG ATCCAAGACC GAACCATTCA CCTGAGAGGC 
    GCATTAAAAG AGCCTATTCA TATACCTAAA ACAGCTGCTG CTACAAAACA GAAACCTGCC 
    AAAAGAGATG GAGAAGACCT GGACAGTCAA GGAGATGGCA GCAGCCAACC TGATACAATA 
    TCTATTGCTT CACGAACTTC ACAGAACACA TTAGACAGTG ATAAGCTTTC TGGTGGATGT 
    GAGCTGACAG TAGTCATCCA TGACTTTACT GCCTGTAATA GCAATGAGCT GACAATTAAA 
    CGAGGGCAGA CTGTGGAGGT TCTAGAGCGG CATCACGATC GACCTGACTG GTGTTTAGTT 
    CGGACTACTG ACCGGTCACC TGCAGCAGAG GGCTTGGTGC CTAGTAGTTC ACTGTGTATT 
    GCACATTCTA GAAGTAGCAT GGAAATGGAA GGCATATTTA ACCACAAAGA TTCCCTCTCT 
    GTCTCCAGTA ATGATGCCAG TCCTCCTGCT TCAGTAATTT CCCTTCAGCC TCACATGATT 
    GGTGCTCAAA GCTCTCCTGG CCCAAAGCGA CCTGGCAATA CTTTGAGAAA ATGGCTCACC 
    AGCCCTGTCA GGAGACTTAG CAGTGGCAAA GCTGATGGAC ATGTGAAAAA ATTGGCTCAT 
    AAACACAAAA AAAGCAGGGA GGTTCGTAAA AACACAGACT CAGGTTCCCA AAAGGACTCT 
    GATGACAGTG CAGCAACACC TCAAGATGAA ACTATCGAGG AGAGAGTGCG CAATGAAGGA 
    CTTAGCAGTG GTACGCTTTC TAAATCCTCC TCGTCTGGCA TGCAAAGCTG TGGAGAAGAA 
    GAGGGCGAGG AAGGACCTGA TGCAGTTCCA TTGCCTCCAC CCATGGCTAT TCAGCAGCAC 
    AGCCTTCTTC AGCCTGACTC ACAGGATGAC AAGACATCAT CTCGGTTGCT GGTGCGACCT 
    ACTAGCTCAG AGACACCTAG TGCTGCAGAG CTTGTTAGTG CTATTGAAGA ACTTGTAAAG 
    AGTAAAATGG CTTTGGAAGA CCGGCCAAGT TCACTTCAAG TAGACCAGGG AGACAGTAGT 
    AGCCCATCAT TCAACCCTTC AGATACATCT CTGCTTTCCT CCTCTTCTCC AATTGATGAA 
    ATGGAAGAAA AAAAATCAAG CTCTTTAAAA AGAAGGCACT ATGTATTACA AGAGCTAGTA 
    GAAACTGAAC GCGATTATGT TCGGGACCTT GGCTATGTGG TTGAGGGTTA TATGGCACTT 
    ATGAAGGAGG ATGGAGTTCC AGATGATATG AAAGGAAAAG ACAAGATTGT TTTTGGCAAC 
    TTACACCAGA TCTACGAGTG GCACAGAGAC TTTTTTTTGG GTGAACTGGA AAGGTGCCTG 
    GATGAGCCTG AAAGATTGGC ATCCCTATTT GTCAAGCATG AAAGAAGATT GCATATGTAC 
    ATAGTTTACT GCCAAAATAA ACCAAAGTCT GAACATATTG TTTCAGAATA CATTGATACC 
    TATTTTGAGG ACCTGAAACA GCGTCTGGGC CATCGACTTC AGCTTACAGA TCTGTTAATA 
    AAACCCATAC AAAGAATAAT GAAATATCAG CTGCTATTAA AGGATTTTCT CAAGTACTCT 
    AAGAAGGCTT GCATAGATGT AGCAGAACTT GAGAGAGCTG TGGAAGTCAT GTGTATCGTA 
    CCGAAGCGGT GTAATGACAT GATGAATGTT GGTCGTTTGC AAGGATTTGA TGGCAAAATA 
    GTTGCCCAAG GAAAGCTGCT ACTGCAAGAC ACATTCCTTG TGACTGAGCA GGACGCTGGT 
    CTTCTGCCCC GATGCAAAGA AAGGCGGGTC TTCCTTTTCG AACAAATTGT CATCTTTAGT 
    GAGCCCTTAG ATAAAAAGAA AGGATACTCT ACACCTGGAT TTCTTTTTAA AAATAATATC 
    AAAGTGAGCT GCCTTAGTTT AGAGGAGTGT GTTGATAGTG ATCCATGCAA GTTTGCACTA 
    TTGTCAAGGT CTGCTGACTC TACAGAGATG TTTGTTTTGC ATTCTTCATC GCCTGGAGTG 
    AGACAGTTAT GGATTCATGA AATCAACCAA ATTTTGGAAA ACCAGCGCAA TTTTTTAAAT 
    GCCTTGACAT CACCAATTGA ATACCACAGA AATCACACTG GTGGAAGCAG TAGTGGCAAC 
    GTCACCTTTA CCAGCAGTCC CAGCAATTGT AGTGGGCTGG GTGGCTCCAG CCGAAGCAGG 
    CCCTCTCGGA TTCCCCAGCC TGTTAGACAC CACTCTCCAG TCCTGGTCTC CTCTGCAGCT 
    TCGGCACAGT CAGAGGCAGA CAAGATGTCA GGTATGTCAG CTCGCCGTGC ACCAGTTGTA 
    CCTCTCAACC TCTCCTTAGA CAATAACCCC AGCCAGAAAG GTAGAGTAAC TGCTGCTCCA 
    GAGACAGGCG GGCGAGATAA AGAATCCAAT TCAGTGCCAA AGATGAAAAT GCTTGATGGC 
    CCCAGGAAAT CAGCTAGTGG CATTCCAACC TTAGCTGATG GTGCACAGAA AGAATTGCGT 
    GGGAGCACAG AAGACAGTCG AATTAGAAGC ACTATGGGCA CTCTAACCTT AGGAAAGCCA 
    AGAAATGGTT CTGCCTCACC CCTCAACTCT CCTGTCACAA TTAATTTTCC ATCGTCTTTT 
    GGTAAGGAAT CAGGGCCACC AAGCAGTCCC CTTCAGAAAG GCTCTTTTTG GAGCTCAATT 
    CCAGGATCTC CAGCCAGCCG ACCTGGCTCC TTCACTTTCC CTGGAGACAG TGACTCTCTC 
    CAGCGACAAG TTCATCGGCA CTCCACACAC AGCAAGGATA CAGACCGCAT GAGCACCTGC 
    TCCTCGACTA GTGAGCAGTC CGTTCACTCC ACTCAGAGTA ATGGGAGTGA AAGTAGCAGC 
    AGTAGCAATA TCTCCACCAT GCTGGTTACG CACGATTATA CAGCGGTGAA GGAGGATGAG 
    ATCAATGTCT ACCAGGGAGA GGTTGTACAA ATTCTGGCAA GTAACCAACA GAACATGTTT 
    CTGGTGTTCA GAGCTGCCAC TGATGAGTGC CCTGCAGCTG AGGGCTGGAT TCCAAGCTAT 
    GTTCTGGGTC ACACAAGTGC AATCATCACT GATATGACTG ATGCAACTAT GAAGAAGTCC 
    TCTTCTTGGC ATACAGCCCT TAGAATAAGA AAGAAATCTG AGAAGAGGGA AAAAGATGGC 
    AGCAGAGACA CAAAACTAGA GAATGGTTAT CGCAAATCAA GAGAAGGACT AGCTAATAAG 
    GTATCAGTTA AGCTCCTGAA TCCAAACTAC ATTTATGATG CTCCTCCAGA GTTTATTGTA 
    CCACTGAACG AGGTTACATG TGACACAGGA GAGACTGTGA TTATTCGATG CAAAGTTTGT 
    GGTCGCCCCA AGGCATCCAT TACATGGAAA GGTCCCGACC ATAATACTTT GAGCAACGAT 
    GGACACTACA GTATATCCTA CAGTGATCTT GGAGAAGCAA CTCTAAAAAT AGTTGGTGTG 
    ACCACTGAGG ATGATGGTAT ATACACCTGC ATTGCAACAA ATGACTTGGG TTCAGTATCC 
    GCTTCCTCCA ATCTTAGAGT GTTAGGTTCA TGGAGTGATG GACTGAGAGT AATCTGGAAA 
    GAAAACTTCG ACATTGCTTA TAGTGAAGTG GCTGAACTTG GCAGGGGCAG GTTTTCTGTC 
    GTGAAAAAAT GTGAACAAAG AGTTACAAGA CGCATAGTAG CCACCAAATT TGTTAACAAG 
    AAGTTGATGA AAAGAGACCA GGTTACCCAT GAACTTGGAA TTCTGCAGAG CCTTCAGCAC 
    CCACAACTGA TCAGCCTTCT TGACACTTTT GAGACACCAG CAAGCTATGT CTTAGTACTT 
    GAGATAGCTG ATCAGGGATG TTTGTTAGAA TATGTGGTAC AGTGGGGAAA CCTTACAGAA 
    GAAAAGATCA CCTCATATAT GAGAGAGGTG CTGGAGGCCC TGCAGTATCT TCATAATTGC 
    AGAATAGCAC ATCTGGACCT AAAGCCTGAG AACATTCTTG TGGATCAGAG CCTAACCAAA 
    CCAACTATAA AACTGTCAGA CTTTGGCGAT GCTGTTCAGC TTAATACCAC TTACTACATT 
    CACCAATTGT TGGGCAGTGC AGAGTTTGCC GCTCCAGAGA TCATCCTTGG GAATCCTGTC 
    TCCCTTACCT CTGATATATG GAGTATTGGA GTTCTTACTT ATGTCCTTCT CAGTGGCGTC 
    TCTCCTTTCC TTGATGAAAG TGTTGAAGAG ACATGCCTGA ACATTTGCCG ACTAGACTTT 
    AGTTTTCCCG ATGACTACTT TAAGGGAGTA AGTCAGAAGG CCAAAGAATT TGTATGTTTC 
    TTACTGCAGG GTGACCAGAC AAAGCGCCCT TCTGCTGCTG TATGTCTAAA GGAACAGTGG 
    TTGCAGCCAG GCCAGCACAA ACGTAGTGGC ACATTAGACA TGTCAAGACT GACCTCGTTT 
    ATTGAACGCC GTAAACACCA GAATGATGTT CGACCCATTG GTAGCATTAA AAACTTCCTT 
    CAGAGTCGCC TTTTACCCAG AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123393.1
    CDS176..9322
    Translation

    Target ORF information:

    RefSeq Version NM_001129921.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001129921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGCAGACTC ATCGCTGGAA AGATGACGGA GAAACAACAT TAGGTTTTCG TAGAAATGAT 
    GAATTGAAAG CAATGGAGGT GTTGCCAATT TTAAAAGAAA AAGTGGCATA TCTATCAGGT 
    GGCAGAGATA AACGAGGTGG TCCTGTATTA ACATTTCCAG CACGCAGCAA CCATGATCGA 
    ATACGACAAG AGGATCTCCG AAGACTGATT TCATATCTTG CTTGTATTCC TAGTGAGGAG 
    GTGTGCAAGC GAGGCTTCAC AGTGATTGTA GATATGCGTG GTTCCAAGTG GGATTCTATT 
    AAACCCTTGC TGAAAATTTT ACAAGAATCC TTTCCATGCT GTATTCATGT TGCACTAATT 
    ATCAAACCAG ATAACTTCTG GCAGAAGCAA AGAACTAATT TTGGAAGTTC TAAGTTTGAA 
    TTTGAGACAA GTATGGTGTC TTTAGAAGGC CTTACCAAAG TAGTTGACCC TTCCCAGCTA 
    ACTCCAGAAT TTGAGGGTTG CTTGGAATAC AACCATGAAG AGTGGATAGA GATCAGAGTA 
    GCATTTGAGG ATTTTATTAA TAATGCAACT CATATGCTGT CTCGATTAGA GGAATTACAG 
    GAGATGCTTG CAATTAAAGA GTTGCCTCAG GATTTAGAGG GGGCACGCAA TATGATTGAA 
    GAACATTCTC AAATGAAAAA AAGGGTTATA AAGGCTCCTA TTGAAGATCT TGATTTAGAA 
    GGGCAAAAGC TTTTACAAAC AATACAAAGC AGTGATAGCT TTCCTAAGAA GAACTCTAGT 
    TCTGGAAATG CAGACCTTCA AAATCTTCTT CCCAAAGTTT CCACAATGTT AGACAGGCTT 
    CATTCAACAC GACAACATCT TCATCAGATG TGGCACATGC GAAAAGTAAA ACTGGACCAG 
    TGCTTTCAGC TAAGGTTGTT TGAGCAGGAT GCTGAGAAGA TGTTTGACTG GATTACACAC 
    AACAAAGGTC TGTTTCTGAC AAGTTATACT GACATTGGCA CCCGTAATAA TCATGCTATG 
    GAACTTCAGA CACAGCACAA TCATTTTGCT ATGAACTGCA TGAATGTCTA TGTAAACATT 
    AGCCGAATAA TGTCTGTGGC AAATCGACTA GTAGAATCTG GACACTATGC CTCACAGCAA 
    ATTAAGCAGA TAGCTAACCA GTTGGAGCAA GAGTGGAAGG CTTTTGCCGC AGCCCTTGAT 
    GAACGTAGTA CGTTGCTTGA CATGTCTGCA ATGTTCCATC AAAAAGCGGA GCAGTACATG 
    GGCAATGTGG ATTCATGGTG CAAAGCTTGT GGGGAAGTGG ATCTTCCATC AGAGCTGCAG 
    GATCTAGAAG ATGCCATTCA TCACCATCAG GGAATATATG AGCATATCAC TTTAGCATAT 
    TCAGAGGTTA GCCAGGATGG TAAATCTTTA TTAGACAAAC TCCAGAGACC ATTGACTCCT 
    GGTAGTTCTG ACTCACTGAC AGCTTCTGCC AACTATTCAA AGGCTGTGCA TCACGTCTTA 
    GATGCAATAC ATGAAGTCTT GCACCACCAG CGTCAGTTGG AAAACATCTG GCAACATCGC 
    AAAGTTCGTC TGCATCAACG CCTACAGCTC TGTGTTTTTC AGCAAGATGT TCAGCAGGTC 
    TTGGATTGGA TAGAGAACCA TGGTGAAGCC TTTCTCAGCA AACACACTGG GGTAGGCAAG 
    TCTCTGCACA GGGCCAGAGC CTTGCAAAAG CGACATGAAG ACTTTGAAGA AGTCGCTCAG 
    AATACATACA CCAATGCTGA TAAGCTGCTG GAAGCTGCAG AACAGTTGGC CCAGACAGGA 
    GAGTGCGATC CAGAGGAAAT ATACCAGGCT GCTCATCAGC TTGAGGATCG GATTCAGGAT 
    TTTGTAAGAC GTGTAGAACA AAGAAAAGTT CTCTTGGACA TGTCTGTATC ATTCCACTCG 
    CATGTTAAAG AGCTATGGAC ATGGCTGGAG GACCTACAAA AGGAACTGCT GGATGATGTC 
    TATGCGGAGT CAGTAGAAGC TGTACAGGAC TTGATCAAAC GGTTTGGACA ACAGCAGCAG 
    ACAACCCTCC AGTTTACAGT AAATGTCATC AAAGAAGGGG AGGATCTAAT TCAGCAACTA 
    AGAGATTCTG CTATATCCAG CAACAAAACT CCTCACAACA GCTCAATCAA TCACATTGAG 
    ACTGTCCTTC AACAACTTGA TGAGGCCCAA TCACAGATGG AGGAGCTTTT CCAAGAGAGA 
    AAAATCAAAC TTGAGCTATT CTTGCAGCTT CGCATCTTTG AGAGAGATGC CATTGATATC 
    ATCTCAGACT TGGAATCATG GAATGATGAA CTTTCTCAGC AAATGAATGA TTTTGACACA 
    GAGGACCTCA CACTAGCCGA GCAAAGGCTA CAGCATCACG CAGACAAAGC TCTGACAATG 
    AACAACCTGA CATTTGATGT CATCCACCAA GGACAAGAGT TACTACAATA TGTGAATGAA 
    GTTCAGAACT CAGGGGTTGA GCTACTCTGT GATAGAGATG TAGATATGGC TACTAGAGTT 
    CAGGACCTGT TGGAATTCCT CCATGAGAAA CAACAGGAGT TGGATCTAGC AGCTGAGCAG 
    CACCGAAAAC ATCTAGAACA ATGTGTGCAA CTACGACATT TGCAAGCTGA AGTCAAACAG 
    GTACTCGGCT GGATACGGAA TGGAGAGTCT ATGTTAAATG CCGGGCTTAT CACTGCCAGT 
    TCATTGCAAG AAGCAGAGCA ACTTCAAAGG GAACATGAGC AGTTCCAGCA TGCAATAGAA 
    AAAACCCACC AGAGTGCTCT GCAGGTGCAG CAGAAGGCAG AGGCCATGCT TCAAGCAAAC 
    CATTACGACA TGGATATGAT CCGGGACTGT GCTGAAAAAG TGGCCTCTCA TTGGCAACAG 
    TTGATGCTTA AAATGGAAGA CCGTCTTAAG CTTGTCAATG CCTCTGTGGC CTTTTATAAA 
    ACCTCAGAGC AGGTATGCAG TGTTCTAGAG AGCCTAGAGC AGGAGTACAA GCGGGAGGAA 
    GATTGGTGTG GCGGAGCTGA TAAACTTGGG CCTAACTCAG AGACTGACCA TGTTACTCCT 
    ATGATCAGCA AACACCTTGA ACAAAAGGAA GCCTTTCTTA AGGCCTGCAC TTTGGCTCGC 
    AGAAATGCTG ATGTCTTTCT GAAATACTTG CACAGGAATA GTGTCAATAT GCCTGGAATG 
    GTGACACATG TAAAGGCTCC TGAACAGCAA GTAAAAAATA TTTTAAATGA GCTGTTTCAG 
    AGGGAAAATA GAGTTCTTCA TTATTGGACC TTGCGGAAAA GGAGACTAGA CCATTGCCAG 
    CAGTATGTAG TATTTGAAAG AAGTGCCAAA CAGGCTCTGG AGTGGATCCA AGATAATGGA 
    GAATTTTACA TTTCCACACA CACCTCCACT GGCTCCACAA TTCAGCATAC ACAAGAACTC 
    TTAAAAGAGC ATGAGGAGTT TCAGATCACA GCAAAGCAAA CCAAAGAGAG GGTAAAGCTG 
    TTGATACAGC TGGCTGATGG CTTTTGTGAA AAAGGGCATG CTCATGCTGC AGAAATTAAA 
    AAATGTGTTA CAGCGGTGGA CAAGAGGTAC AGAGACTTCT CGCTGCGTAT GGAAAAGTAC 
    AGAACCAGCT TAGAGAAGGC TTTGGGCATT TCTTCTGATT CCAAGTCGAG TAAAAGCCTT 
    CAGTTGGATA TTATTCCAGC CACTGCCCCT GGTTCCGAAG TCAAGCTTCG TGATGCAACA 
    CATGAACTCA ATGAGGAGAA AAGAAAATCT GCTCGTCGAA AAGAATTCAT CATGGCAGAA 
    CTTATACAAA CTGAAAAAGC ATATGTTCGT GATCTTCGGG AATGCATGGA TACATATCTG 
    TGGGAAATGA CAAGCGGAGT TGAAGAGATC CCACCAGGCA TTGTAAATAA AGAACACATT 
    ATCTTTGGAA ACATGCAGGA AATATATGAA TTTCATAACA ACATATTCTT AAAGGAGCTT 
    GAGAAGTATG AGCAGCTACC TGAAGATGTT GGACATTGTT TTGTCACATG GGCTGACAAA 
    TTTCAGATGT ATGTGTCCTA CTGTAAGAAT AAGCCTGATT CAACACAACT TATCTTGGAA 
    CAAGCAGGAA CATATTTTGA TGAAATTCAG CAGCGCCATG AGCTGGTCAA CTCCATCTCT 
    TCTTATCTTA TCAAACCTGT GCAAAGGATA ACAAAATATC AGCTTCTTTT AAAAGAGCTG 
    CTGACATGCT GCGAAGAAGG TAAAGGAGAG ATTAAAGATG GGCTGGAAGT GATGCTAAGT 
    GTACCAAAAA GAGCCAACGA TGCAATGCAT CTCAGCATGC TTGAAGGGTT TGATGAGAAC 
    ATTGAATCTC AGGGAGAACT TATTCTGCAA GAGTCTTTTC AAGTGTGGGA TCCTAAAACC 
    TTAATAAGAA AGGGGCGTGA GAGACATCTC TTCTTGTTTG AAATGTCTTT GGTCTTCAGC 
    AAAGAAGTGA AAGATTCAAG TGGAAGGAGC AAATATATTT ACAAATCAAA ATTATTTACC 
    TCAGAGCTTG GAGTCACAGA ACATGTAGAA GGTGATCCTT GCAAATTTGC ACTTTGGGTA 
    GGCAGAACGC CAACATCTGA TAACAAAATA GTCCTTAAGG GCTCAAGTAT TGAGAACAAG 
    CAAGATTGGA TCAAGCATAT AAGAGAAGTG ATCCAAGACC GAACCATTCA CCTGAGAGGC 
    GCATTAAAAG AGCCTATTCA TATACCTAAA ACAGCTGCTG CTACAAAACA GAAACCTGCC 
    AAAAGAGATG GAGAAGACCT GGACAGTCAA GGAGATGGCA GCAGCCAACC TGATACAATA 
    TCTATTGCTT CACGAACTTC ACAGAACACA TTAGACAGTG ATAAGCTTTC TGGTGGATGT 
    GAGCTGACAG TAGTCATCCA TGACTTTACT GCCTGTAATA GCAATGAGCT GACAATTAAA 
    CGAGGGCAGA CTGTGGAGGT TCTAGAGCGG CATCACGATC GACCTGACTG GTGTTTAGTT 
    CGGACTACTG ACCGGTCACC TGCAGCAGAG GGCTTGGTGC CTAGTAGTTC ACTGTGTATT 
    GCACATTCTA GAAGTAGCAT GGAAATGGAA GGCATATTTA ACCACAAAGA TTCCCTCTCT 
    GTCTCCAGTA ATGATGCCAG TCCTCCTGCT TCAGTAATTT CCCTTCAGCC TCACATGATT 
    GGTGCTCAAA GCTCTCCTGG CCCAAAGCGA CCTGGCAATA CTTTGAGAAA ATGGCTCACC 
    AGCCCTGTCA GGAGACTTAG CAGTGGCAAA GCTGATGGAC ATGTGAAAAA ATTGGCTCAT 
    AAACACAAAA AAAGCAGGGA GGTTCGTAAA AACACAGACT CAGGTTCCCA AAAGGACTCT 
    GATGACAGTG CAGCAACACC TCAAGATGAA ACTATCGAGG AGAGAGTGCG CAATGAAGGA 
    CTTAGCAGTG GTACGCTTTC TAAATCCTCC TCGTCTGGCA TGCAAAGCTG TGGAGAAGAA 
    GAGGGCGAGG AAGGACCTGA TGCAGTTCCA TTGCCTCCAC CCATGGCTAT TCAGCAGCAC 
    AGCCTTCTTC AGCCTGACTC ACAGGATGAC AAGACATCAT CTCGGTTGCT GGTGCGACCT 
    ACTAGCTCAG AGACACCTAG TGCTGCAGAG CTTGTTAGTG CTATTGAAGA ACTTGTAAAG 
    AGTAAAATGG CTTTGGAAGA CCGGCCAAGT TCACTTCAAG TAGACCAGGG AGACAGTAGT 
    AGCCCATCAT TCAACCCTTC AGATACATCT CTGCTTTCCT CCTCTTCTCC AATTGATGAA 
    ATGGAAGAAA AAAAATCAAG CTCTTTAAAA AGAAGGCACT ATGTATTACA AGAGCTAGTA 
    GAAACTGAAC GCGATTATGT TCGGGACCTT GGCTATGTGG TTGAGGGTTA TATGGCACTT 
    ATGAAGGAGG ATGGAGTTCC AGATGATATG AAAGGAAAAG ACAAGATTGT TTTTGGCAAC 
    TTACACCAGA TCTACGAGTG GCACAGAGAC TTTTTTTTGG GTGAACTGGA AAGGTGCCTG 
    GATGAGCCTG AAAGATTGGC ATCCCTATTT GTCAAGCATG AAAGAAGATT GCATATGTAC 
    ATAGTTTACT GCCAAAATAA ACCAAAGTCT GAACATATTG TTTCAGAATA CATTGATACC 
    TATTTTGAGG ACCTGAAACA GCGTCTGGGC CATCGACTTC AGCTTACAGA TCTGTTAATA 
    AAACCCATAC AAAGAATAAT GAAATATCAG CTGCTATTAA AGGATTTTCT CAAGTACTCT 
    AAGAAGGCTT GCATAGATGT AGCAGAACTT GAGAGAGCTG TGGAAGTCAT GTGTATCGTA 
    CCGAAGCGGT GTAATGACAT GATGAATGTT GGTCGTTTGC AAGGATTTGA TGGCAAAATA 
    GTTGCCCAAG GAAAGCTGCT ACTGCAAGAC ACATTCCTTG TGACTGAGCA GGACGCTGGT 
    CTTCTGCCCC GATGCAAAGA AAGGCGGGTC TTCCTTTTCG AACAAATTGT CATCTTTAGT 
    GAGCCCTTAG ATAAAAAGAA AGGATACTCT ACACCTGGAT TTCTTTTTAA AAATAATATC 
    AAAGTGAGCT GCCTTAGTTT AGAGGAGTGT GTTGATAGTG ATCCATGCAA GTTTGCACTA 
    TTGTCAAGGT CTGCTGACTC TACAGAGATG TTTGTTTTGC ATTCTTCATC GCCTGGAGTG 
    AGACAGTTAT GGATTCATGA AATCAACCAA ATTTTGGAAA ACCAGCGCAA TTTTTTAAAT 
    GCCTTGACAT CACCAATTGA ATACCACAGA AATCACACTG GTGGAAGCAG TAGTGGCAAC 
    GTCACCTTTA CCAGCAGTCC CAGCAATTGT AGTGGGCTGG GTGGCTCCAG CCGAAGCAGG 
    CCCTCTCGGA TTCCCCAGCC TGTTAGACAC CACTCTCCAG TCCTGGTCTC CTCTGCAGCT 
    TCGGCACAGT CAGAGGCAGA CAAGATGTCA GGTATGTCAG CTCGCCGTGC ACCAGTTGTA 
    CCTCTCAACC TCTCCTTAGA CAATAACCCC AGCCAGAAAG GTAGAGTAAC TGCTGCTCCA 
    GAGACAGGCG GGCGAGATAA AGAATCCAAT TCAGTGCCAA AGATGAAAAT GCTTGATGGC 
    CCCAGGAAAT CAGCTAGTGG CATTCCAACC TTAGCTGATG GTGCACAGAA AGAATTGCGT 
    GGGAGCACAG AAGACAGTCG AATTAGAAGC ACTATGGGCA CTCTAACCTT AGGAAAGCCA 
    AGAAATGGTT CTGCCTCACC CCTCAACTCT CCTGTCACAA TTAATTTTCC ATCGTCTTTT 
    GGTAAGGAAT CAGGGCCACC AAGCAGTCCC CTTCAGAAAG GCTCTTTTTG GAGCTCAATT 
    CCAGGATCTC CAGCCAGCCG ACCTGGCTCC TTCACTTTCC CTGGAGACAG TGACTCTCTC 
    CAGCGACAAG TTCATCGGCA CTCCACACAC AGCAAGGATA CAGACCGCAT GAGCACCTGC 
    TCCTCGACTA GTGAGCAGTC CGTTCACTCC ACTCAGAGTA ATGGGAGTGA AAGTAGCAGC 
    AGTAGCAATA TCTCCACCAT GCTGGTTACG CACGATTATA CAGCGGTGAA GGAGGATGAG 
    ATCAATGTCT ACCAGGGAGA GGTTGTACAA ATTCTGGCAA GTAACCAACA GAACATGTTT 
    CTGGTGTTCA GAGCTGCCAC TGATGAGTGC CCTGCAGCTG AGGGCTGGAT TCCAAGCTAT 
    GTTCTGGGTC ACACAAGTGC AATCATCACT GATATGACTG ATGCAACTAT GAAGAAGTCC 
    TCTTCTTGGC ATACAGCCCT TAGAATAAGA AAGAAATCTG AGAAGAGGGA AAAAGATGGC 
    AGCAGAGACA CAAAACTAGA GAATGGTTAT CGCAAATCAA GAGAAGGACT AGCTAATAAG 
    GTATCAGTTA AGCTCCTGAA TCCAAACTAC ATTTATGATG CTCCTCCAGA GTTTATTGTA 
    CCACTGAACG AGGTTACATG TGACACAGGA GAGACTGTGA TTATTCGATG CAAAGTTTGT 
    GGTCGCCCCA AGGCATCCAT TACATGGAAA GGTCCCGACC ATAATACTTT GAGCAACGAT 
    GGACACTACA GTATATCCTA CAGTGATCTT GGAGAAGCAA CTCTAAAAAT AGTTGGTGTG 
    ACCACTGAGG ATGATGGTAT ATACACCTGC ATTGCAACAA ATGACTTGGG TTCAGTATCC 
    GCTTCCTCCA ATCTTAGAGT GTTAGGTTCA TGGAGTGATG GACTGAGAGT AATCTGGAAA 
    GAAAACTTCG ACATTGCTTA TAGTGAAGTG GCTGAACTTG GCAGGGGCAG GTTTTCTGTC 
    GTGAAAAAAT GTGAACAAAG AGTTACAAGA CGCATAGTAG CCACCAAATT TGTTAACAAG 
    AAGTTGATGA AAAGAGACCA GGTTACCCAT GAACTTGGAA TTCTGCAGAG CCTTCAGCAC 
    CCACAACTGA TCAGCCTTCT TGACACTTTT GAGACACCAG CAAGCTATGT CTTAGTACTT 
    GAGATAGCTG ATCAGGGATG TTTGTTAGAA TATGTGGTAC AGTGGGGAAA CCTTACAGAA 
    GAAAAGATCA CCTCATATAT GAGAGAGGTG CTGGAGGCCC TGCAGTATCT TCATAATTGC 
    AGAATAGCAC ATCTGGACCT AAAGCCTGAG AACATTCTTG TGGATCAGAG CCTAACCAAA 
    CCAACTATAA AACTGTCAGA CTTTGGCGAT GCTGTTCAGC TTAATACCAC TTACTACATT 
    CACCAATTGT TGGGCAGTGC AGAGTTTGCC GCTCCAGAGA TCATCCTTGG GAATCCTGTC 
    TCCCTTACCT CTGATATATG GAGTATTGGA GTTCTTACTT ATGTCCTTCT CAGTGGCGTC 
    TCTCCTTTCC TTGATGAAAG TGTTGAAGAG ACATGCCTGA ACATTTGCCG ACTAGACTTT 
    AGTTTTCCCG ATGACTACTT TAAGGGAGTA AGTCAGAAGG CCAAAGAATT TGTATGTTTC 
    TTACTGCAGG GTGACCAGAC AAAGCGCCCT TCTGCTGCTG TATGTCTAAA GGAACAGTGG 
    TTGCAGCCAG GCCAGCACAA ACGTAGTGGC ACATTAGACA TGTCAAGACT GACCTCGTTT 
    ATTGAACGCC GTAAACACCA GAATGATGTT CGACCCATTG GTAGCATTAA AAACTTCCTT 
    CAGAGTCGCC TTTTACCCAG AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_012964353.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819807.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa32253
    Clone ID Related Accession (Same CDS sequence) XM_012964353.3 , XM_012964353.2
    Accession Version XM_012964353.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    1021
    1081
    1141
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    1321
    1381
    1441
    1501
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    1801
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    1921
    1981
    2041
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    2641
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    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT 
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC 
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC 
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC 
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG 
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC 
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC 
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA 
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG 
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG 
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATAGCT 
    GATCAGGGAT GTTTGTTAGA ATATGTGGTA CAGTGGGGAA ACCTTACAGA AGAAAAGATC 
    ACCTCATATA TGAGAGAGGT GCTGGAGGCC CTGCAGTATC TTCATAATTG CAGAATAGCA 
    CATCTGGACC TAAAGCCTGA GAACATTCTT GTGGATCAGA GCCTAACCAA ACCAACTATA 
    AAACTGTCAG ACTTTGGCGA TGCTGTTCAG CTTAATACCA CTTACTACAT TCACCAATTG 
    TTGGGCAGTG CAGAGTTTGC CGCTCCAGAG ATCATCCTTG GGAATCCTGT CTCCCTTACC 
    TCTGATATAT GGAGTATTGG AGTTCTTACT TATGTCCTTC TCAGTGGCGT CTCTCCTTTC 
    CTTGATGAAA GTGTTGAAGA GACATGCCTG AACATTTGCC GACTAGACTT TAGTTTTCCC 
    GATGACTACT TTAAGGGAGT AAGTCAGAAG GCCAAAGAAT TTGTATGTTT CTTACTGCAG 
    GGTGACCAGA CAAAGCGCCC TTCTGCTGCT GTATGTCTAA AGGAACAGTG GTTGCAGCCA 
    GGCCAGCACA AACGTAGTGG CACATTAGAC ATGTCAAGAC TGACCTCGTT TATTGAACGC 
    CGTAAACACC AGAATGATGT TCGACCCATT GGTAGCATTA AAAACTTCCT TCAGAGTCGC 
    CTTTTACCCA GAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819807.1
    CDS527..9784
    Translation

    Target ORF information:

    RefSeq Version XM_012964353.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964353.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT 
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC 
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC 
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC 
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG 
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC 
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC 
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA 
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG 
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG 
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATAGCT 
    GATCAGGGAT GTTTGTTAGA ATATGTGGTA CAGTGGGGAA ACCTTACAGA AGAAAAGATC 
    ACCTCATATA TGAGAGAGGT GCTGGAGGCC CTGCAGTATC TTCATAATTG CAGAATAGCA 
    CATCTGGACC TAAAGCCTGA GAACATTCTT GTGGATCAGA GCCTAACCAA ACCAACTATA 
    AAACTGTCAG ACTTTGGCGA TGCTGTTCAG CTTAATACCA CTTACTACAT TCACCAATTG 
    TTGGGCAGTG CAGAGTTTGC CGCTCCAGAG ATCATCCTTG GGAATCCTGT CTCCCTTACC 
    TCTGATATAT GGAGTATTGG AGTTCTTACT TATGTCCTTC TCAGTGGCGT CTCTCCTTTC 
    CTTGATGAAA GTGTTGAAGA GACATGCCTG AACATTTGCC GACTAGACTT TAGTTTTCCC 
    GATGACTACT TTAAGGGAGT AAGTCAGAAG GCCAAAGAAT TTGTATGTTT CTTACTGCAG 
    GGTGACCAGA CAAAGCGCCC TTCTGCTGCT GTATGTCTAA AGGAACAGTG GTTGCAGCCA 
    GGCCAGCACA AACGTAGTGG CACATTAGAC ATGTCAAGAC TGACCTCGTT TATTGAACGC 
    CGTAAACACC AGAATGATGT TCGACCCATT GGTAGCATTA AAAACTTCCT TCAGAGTCGC 
    CTTTTACCCA GAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_012964353.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819807.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa32253
    Clone ID Related Accession (Same CDS sequence) XM_012964353.3 , XM_012964353.2
    Accession Version XM_012964353.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT 
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC 
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC 
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC 
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG 
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC 
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC 
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA 
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG 
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG 
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATAGCT 
    GATCAGGGAT GTTTGTTAGA ATATGTGGTA CAGTGGGGAA ACCTTACAGA AGAAAAGATC 
    ACCTCATATA TGAGAGAGGT GCTGGAGGCC CTGCAGTATC TTCATAATTG CAGAATAGCA 
    CATCTGGACC TAAAGCCTGA GAACATTCTT GTGGATCAGA GCCTAACCAA ACCAACTATA 
    AAACTGTCAG ACTTTGGCGA TGCTGTTCAG CTTAATACCA CTTACTACAT TCACCAATTG 
    TTGGGCAGTG CAGAGTTTGC CGCTCCAGAG ATCATCCTTG GGAATCCTGT CTCCCTTACC 
    TCTGATATAT GGAGTATTGG AGTTCTTACT TATGTCCTTC TCAGTGGCGT CTCTCCTTTC 
    CTTGATGAAA GTGTTGAAGA GACATGCCTG AACATTTGCC GACTAGACTT TAGTTTTCCC 
    GATGACTACT TTAAGGGAGT AAGTCAGAAG GCCAAAGAAT TTGTATGTTT CTTACTGCAG 
    GGTGACCAGA CAAAGCGCCC TTCTGCTGCT GTATGTCTAA AGGAACAGTG GTTGCAGCCA 
    GGCCAGCACA AACGTAGTGG CACATTAGAC ATGTCAAGAC TGACCTCGTT TATTGAACGC 
    CGTAAACACC AGAATGATGT TCGACCCATT GGTAGCATTA AAAACTTCCT TCAGAGTCGC 
    CTTTTACCCA GAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819807.1
    CDS616..9873
    Translation

    Target ORF information:

    RefSeq Version XM_012964353.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964353.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT 
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC 
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC 
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC 
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG 
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC 
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC 
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA 
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG 
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG 
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATAGCT 
    GATCAGGGAT GTTTGTTAGA ATATGTGGTA CAGTGGGGAA ACCTTACAGA AGAAAAGATC 
    ACCTCATATA TGAGAGAGGT GCTGGAGGCC CTGCAGTATC TTCATAATTG CAGAATAGCA 
    CATCTGGACC TAAAGCCTGA GAACATTCTT GTGGATCAGA GCCTAACCAA ACCAACTATA 
    AAACTGTCAG ACTTTGGCGA TGCTGTTCAG CTTAATACCA CTTACTACAT TCACCAATTG 
    TTGGGCAGTG CAGAGTTTGC CGCTCCAGAG ATCATCCTTG GGAATCCTGT CTCCCTTACC 
    TCTGATATAT GGAGTATTGG AGTTCTTACT TATGTCCTTC TCAGTGGCGT CTCTCCTTTC 
    CTTGATGAAA GTGTTGAAGA GACATGCCTG AACATTTGCC GACTAGACTT TAGTTTTCCC 
    GATGACTACT TTAAGGGAGT AAGTCAGAAG GCCAAAGAAT TTGTATGTTT CTTACTGCAG 
    GGTGACCAGA CAAAGCGCCC TTCTGCTGCT GTATGTCTAA AGGAACAGTG GTTGCAGCCA 
    GGCCAGCACA AACGTAGTGG CACATTAGAC ATGTCAAGAC TGACCTCGTT TATTGAACGC 
    CGTAAACACC AGAATGATGT TCGACCCATT GGTAGCATTA AAAACTTCCT TCAGAGTCGC 
    CTTTTACCCA GAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_012964354.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819808.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa32254
    Clone ID Related Accession (Same CDS sequence) XM_012964354.2 , XM_012964354.3
    Accession Version XM_012964354.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGAGTGAA AGTAGCAGCA GTAGCAATAT CTCCACCATG 
    CTGGTTACGC ACGATTATAC AGCGGTGAAG GAGGATGAGA TCAATGTCTA CCAGGGAGAG 
    GTTGTACAAA TTCTGGCAAG TAACCAACAG AACATGTTTC TGGTGTTCAG AGCTGCCACT 
    GATGAGTGCC CTGCAGCTGA GGGCTGGATT CCAAGCTATG TTCTGGGTCA CACAAGTGCA 
    ATCATCACTG ATATGACTGA TGCAACTATG AAGAAGTCCT CTTCTTGGCA TACAGCCCTT 
    AGAATAAGAA AGAAATCTGA GAAGAGGGAA AAAGATGGCA GCAGAGACAC AAAACTAGAG 
    AATGGTTATC GCAAATCAAG AGAAGGACTA GCTAATAAGG TATCAGTTAA GCTCCTGAAT 
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT 
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT 
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC 
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA 
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG 
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT 
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA 
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG 
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT 
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT 
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG 
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA 
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC 
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA 
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG 
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT 
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT 
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA 
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA 
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG 
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819808.1
    CDS614..9859
    Translation

    Target ORF information:

    RefSeq Version XM_012964354.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964354.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGAGTGAA AGTAGCAGCA GTAGCAATAT CTCCACCATG 
    CTGGTTACGC ACGATTATAC AGCGGTGAAG GAGGATGAGA TCAATGTCTA CCAGGGAGAG 
    GTTGTACAAA TTCTGGCAAG TAACCAACAG AACATGTTTC TGGTGTTCAG AGCTGCCACT 
    GATGAGTGCC CTGCAGCTGA GGGCTGGATT CCAAGCTATG TTCTGGGTCA CACAAGTGCA 
    ATCATCACTG ATATGACTGA TGCAACTATG AAGAAGTCCT CTTCTTGGCA TACAGCCCTT 
    AGAATAAGAA AGAAATCTGA GAAGAGGGAA AAAGATGGCA GCAGAGACAC AAAACTAGAG 
    AATGGTTATC GCAAATCAAG AGAAGGACTA GCTAATAAGG TATCAGTTAA GCTCCTGAAT 
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT 
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT 
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC 
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA 
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG 
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT 
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA 
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG 
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT 
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT 
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG 
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA 
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC 
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA 
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG 
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT 
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT 
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA 
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA 
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG 
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_012964354.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819808.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa32254
    Clone ID Related Accession (Same CDS sequence) XM_012964354.2 , XM_012964354.3
    Accession Version XM_012964354.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964354.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGAGTGAA AGTAGCAGCA GTAGCAATAT CTCCACCATG 
    CTGGTTACGC ACGATTATAC AGCGGTGAAG GAGGATGAGA TCAATGTCTA CCAGGGAGAG 
    GTTGTACAAA TTCTGGCAAG TAACCAACAG AACATGTTTC TGGTGTTCAG AGCTGCCACT 
    GATGAGTGCC CTGCAGCTGA GGGCTGGATT CCAAGCTATG TTCTGGGTCA CACAAGTGCA 
    ATCATCACTG ATATGACTGA TGCAACTATG AAGAAGTCCT CTTCTTGGCA TACAGCCCTT 
    AGAATAAGAA AGAAATCTGA GAAGAGGGAA AAAGATGGCA GCAGAGACAC AAAACTAGAG 
    AATGGTTATC GCAAATCAAG AGAAGGACTA GCTAATAAGG TATCAGTTAA GCTCCTGAAT 
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT 
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT 
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC 
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA 
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG 
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT 
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA 
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG 
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT 
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT 
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG 
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA 
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC 
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA 
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG 
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT 
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT 
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA 
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA 
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG 
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819808.1
    CDS527..9772
    Translation

    Target ORF information:

    RefSeq Version XM_012964354.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964354.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGAGTGAA AGTAGCAGCA GTAGCAATAT CTCCACCATG 
    CTGGTTACGC ACGATTATAC AGCGGTGAAG GAGGATGAGA TCAATGTCTA CCAGGGAGAG 
    GTTGTACAAA TTCTGGCAAG TAACCAACAG AACATGTTTC TGGTGTTCAG AGCTGCCACT 
    GATGAGTGCC CTGCAGCTGA GGGCTGGATT CCAAGCTATG TTCTGGGTCA CACAAGTGCA 
    ATCATCACTG ATATGACTGA TGCAACTATG AAGAAGTCCT CTTCTTGGCA TACAGCCCTT 
    AGAATAAGAA AGAAATCTGA GAAGAGGGAA AAAGATGGCA GCAGAGACAC AAAACTAGAG 
    AATGGTTATC GCAAATCAAG AGAAGGACTA GCTAATAAGG TATCAGTTAA GCTCCTGAAT 
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT 
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT 
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC 
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA 
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG 
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT 
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA 
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG 
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT 
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT 
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG 
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA 
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC 
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA 
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG 
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT 
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT 
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA 
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA 
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG 
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_012964355.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819809.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OXa32255
    Clone ID Related Accession (Same CDS sequence) XM_012964355.2 , XM_012964355.3
    Accession Version XM_012964355.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GCTCCTGAAT 
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT 
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT 
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC 
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA 
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG 
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT 
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA 
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG 
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT 
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT 
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG 
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA 
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC 
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA 
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG 
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT 
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT 
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA 
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA 
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG 
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819809.1
    CDS616..9861
    Translation

    Target ORF information:

    RefSeq Version XM_012964355.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964355.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GCTCCTGAAT 
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT 
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT 
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC 
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA 
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG 
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT 
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA 
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG 
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT 
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT 
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG 
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA 
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC 
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA 
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG 
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT 
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT 
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA 
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA 
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG 
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_012964355.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819809.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OXa32255
    Clone ID Related Accession (Same CDS sequence) XM_012964355.2 , XM_012964355.3
    Accession Version XM_012964355.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GCTCCTGAAT 
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT 
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT 
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC 
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA 
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG 
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT 
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA 
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG 
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT 
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT 
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG 
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA 
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC 
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA 
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG 
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT 
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT 
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA 
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA 
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG 
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819809.1
    CDS527..9772
    Translation

    Target ORF information:

    RefSeq Version XM_012964355.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964355.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GCTCCTGAAT 
    CCAAACTACA TTTATGATGC TCCTCCAGAG TTTATTGTAC CACTGAACGA GGTTACATGT 
    GACACAGGAG AGACTGTGAT TATTCGATGC AAAGTTTGTG GTCGCCCCAA GGCATCCATT 
    ACATGGAAAG GTCCCGACCA TAATACTTTG AGCAACGATG GACACTACAG TATATCCTAC 
    AGTGATCTTG GAGAAGCAAC TCTAAAAATA GTTGGTGTGA CCACTGAGGA TGATGGTATA 
    TACACCTGCA TTGCAACAAA TGACTTGGGT TCAGTATCCG CTTCCTCCAA TCTTAGAGTG 
    TTAGGTTCAT GGAGTGATGG ACTGAGAGTA ATCTGGAAAG AAAACTTCGA CATTGCTTAT 
    AGTGAAGTGG CTGAACTTGG CAGGGGCAGG TTTTCTGTCG TGAAAAAATG TGAACAAAGA 
    GTTACAAGAC GCATAGTAGC CACCAAATTT GTTAACAAGA AGTTGATGAA AAGAGACCAG 
    GTTACCCATG AACTTGGAAT TCTGCAGAGC CTTCAGCACC CACAACTGAT CAGCCTTCTT 
    GACACTTTTG AGACACCAGC AAGCTATGTC TTAGTACTTG AGATAGCTGA TCAGGGATGT 
    TTGTTAGAAT ATGTGGTACA GTGGGGAAAC CTTACAGAAG AAAAGATCAC CTCATATATG 
    AGAGAGGTGC TGGAGGCCCT GCAGTATCTT CATAATTGCA GAATAGCACA TCTGGACCTA 
    AAGCCTGAGA ACATTCTTGT GGATCAGAGC CTAACCAAAC CAACTATAAA ACTGTCAGAC 
    TTTGGCGATG CTGTTCAGCT TAATACCACT TACTACATTC ACCAATTGTT GGGCAGTGCA 
    GAGTTTGCCG CTCCAGAGAT CATCCTTGGG AATCCTGTCT CCCTTACCTC TGATATATGG 
    AGTATTGGAG TTCTTACTTA TGTCCTTCTC AGTGGCGTCT CTCCTTTCCT TGATGAAAGT 
    GTTGAAGAGA CATGCCTGAA CATTTGCCGA CTAGACTTTA GTTTTCCCGA TGACTACTTT 
    AAGGGAGTAA GTCAGAAGGC CAAAGAATTT GTATGTTTCT TACTGCAGGG TGACCAGACA 
    AAGCGCCCTT CTGCTGCTGT ATGTCTAAAG GAACAGTGGT TGCAGCCAGG CCAGCACAAA 
    CGTAGTGGCA CATTAGACAT GTCAAGACTG ACCTCGTTTA TTGAACGCCG TAAACACCAG 
    AATGATGTTC GACCCATTGG TAGCATTAAA AACTTCCTTC AGAGTCGCCT TTTACCCAGA 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_012964356.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819810.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OXa32256
    Clone ID Related Accession (Same CDS sequence) XM_012964356.2
    Accession Version XM_012964356.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    8641
    8701
    ATGGTGTCTT TAGAAGGCCT TACCAAAGTA GTTGACCCTT CCCAGCTAAC TCCAGAATTT 
    GAGGGTTGCT TGGAATACAA CCATGAAGAG TGGATAGAGA TCAGAGTAGC ATTTGAGGAT 
    TTTATTAATA ATGCAACTCA TATGCTGTCT CGATTAGAGG AATTACAGGA GATGCTTGCA 
    ATTAAAGAGT TGCCTCAGGA TTTAGAGGGG GCACGCAATA TGATTGAAGA ACATTCTCAA 
    ATGAAAAAAA GGGTTATAAA GGCTCCTATT GAAGATCTTG ATTTAGAAGG GCAAAAGCTT 
    TTACAAACAA TACAAAGCAG TGATAGCTTT CCTAAGAAGA ACTCTAGTTC TGGAAATGCA 
    GACCTTCAAA ATCTTCTTCC CAAAGTTTCC ACAATGTTAG ACAGGCTTCA TTCAACACGA 
    CAACATCTTC ATCAGATGTG GCACATGCGA AAAGTAAAAC TGGACCAGTG CTTTCAGCTA 
    AGGTTGTTTG AGCAGGATGC TGAGAAGATG TTTGACTGGA TTACACACAA CAAAGGTCTG 
    TTTCTGACAA GTTATACTGA CATTGGCACC CGTAATAATC ATGCTATGGA ACTTCAGACA 
    CAGCACAATC ATTTTGCTAT GAACTGCATG AATGTCTATG TAAACATTAG CCGAATAATG 
    TCTGTGGCAA ATCGACTAGT AGAATCTGGA CACTATGCCT CACAGCAAAT TAAGCAGATA 
    GCTAACCAGT TGGAGCAAGA GTGGAAGGCT TTTGCCGCAG CCCTTGATGA ACGTAGTACG 
    TTGCTTGACA TGTCTGCAAT GTTCCATCAA AAAGCGGAGC AGTACATGGG CAATGTGGAT 
    TCATGGTGCA AAGCTTGTGG GGAAGTGGAT CTTCCATCAG AGCTGCAGGA TCTAGAAGAT 
    GCCATTCATC ACCATCAGGG AATATATGAG CATATCACTT TAGCATATTC AGAGGTTAGC 
    CAGGATGGTA AATCTTTATT AGACAAACTC CAGAGACCAT TGACTCCTGG TAGTTCTGAC 
    TCACTGACAG CTTCTGCCAA CTATTCAAAG GCTGTGCATC ACGTCTTAGA TGCAATACAT 
    GAAGTCTTGC ACCACCAGCG TCAGTTGGAA AACATCTGGC AACATCGCAA AGTTCGTCTG 
    CATCAACGCC TACAGCTCTG TGTTTTTCAG CAAGATGTTC AGCAGGTCTT GGATTGGATA 
    GAGAACCATG GTGAAGCCTT TCTCAGCAAA CACACTGGGG TAGGCAAGTC TCTGCACAGG 
    GCCAGAGCCT TGCAAAAGCG ACATGAAGAC TTTGAAGAAG TCGCTCAGAA TACATACACC 
    AATGCTGATA AGCTGCTGGA AGCTGCAGAA CAGTTGGCCC AGACAGGAGA GTGCGATCCA 
    GAGGAAATAT ACCAGGCTGC TCATCAGCTT GAGGATCGGA TTCAGGATTT TGTAAGACGT 
    GTAGAACAAA GAAAAGTTCT CTTGGACATG TCTGTATCAT TCCACTCGCA TGTTAAAGAG 
    CTATGGACAT GGCTGGAGGA CCTACAAAAG GAACTGCTGG ATGATGTCTA TGCGGAGTCA 
    GTAGAAGCTG TACAGGACTT GATCAAACGG TTTGGACAAC AGCAGCAGAC AACCCTCCAG 
    TTTACAGTAA ATGTCATCAA AGAAGGGGAG GATCTAATTC AGCAACTAAG AGATTCTGCT 
    ATATCCAGCA ACAAAACTCC TCACAACAGC TCAATCAATC ACATTGAGAC TGTCCTTCAA 
    CAACTTGATG AGGCCCAATC ACAGATGGAG GAGCTTTTCC AAGAGAGAAA AATCAAACTT 
    GAGCTATTCT TGCAGCTTCG CATCTTTGAG AGAGATGCCA TTGATATCAT CTCAGACTTG 
    GAATCATGGA ATGATGAACT TTCTCAGCAA ATGAATGATT TTGACACAGA GGACCTCACA 
    CTAGCCGAGC AAAGGCTACA GCATCACGCA GACAAAGCTC TGACAATGAA CAACCTGACA 
    TTTGATGTCA TCCACCAAGG ACAAGAGTTA CTACAATATG TGAATGAAGT TCAGAACTCA 
    GGGGTTGAGC TACTCTGTGA TAGAGATGTA GATATGGCTA CTAGAGTTCA GGACCTGTTG 
    GAATTCCTCC ATGAGAAACA ACAGGAGTTG GATCTAGCAG CTGAGCAGCA CCGAAAACAT 
    CTAGAACAAT GTGTGCAACT ACGACATTTG CAAGCTGAAG TCAAACAGGT ACTCGGCTGG 
    ATACGGAATG GAGAGTCTAT GTTAAATGCC GGGCTTATCA CTGCCAGTTC ATTGCAAGAA 
    GCAGAGCAAC TTCAAAGGGA ACATGAGCAG TTCCAGCATG CAATAGAAAA AACCCACCAG 
    AGTGCTCTGC AGGTGCAGCA GAAGGCAGAG GCCATGCTTC AAGCAAACCA TTACGACATG 
    GATATGATCC GGGACTGTGC TGAAAAAGTG GCCTCTCATT GGCAACAGTT GATGCTTAAA 
    ATGGAAGACC GTCTTAAGCT TGTCAATGCC TCTGTGGCCT TTTATAAAAC CTCAGAGCAG 
    GTATGCAGTG TTCTAGAGAG CCTAGAGCAG GAGTACAAGC GGGAGGAAGA TTGGTGTGGC 
    GGAGCTGATA AACTTGGGCC TAACTCAGAG ACTGACCATG TTACTCCTAT GATCAGCAAA 
    CACCTTGAAC AAAAGGAAGC CTTTCTTAAG GCCTGCACTT TGGCTCGCAG AAATGCTGAT 
    GTCTTTCTGA AATACTTGCA CAGGAATAGT GTCAATATGC CTGGAATGGT GACACATGTA 
    AAGGCTCCTG AACAGCAAGT AAAAAATATT TTAAATGAGC TGTTTCAGAG GGAAAATAGA 
    GTTCTTCATT ATTGGACCTT GCGGAAAAGG AGACTAGACC ATTGCCAGCA GTATGTAGTA 
    TTTGAAAGAA GTGCCAAACA GGCTCTGGAG TGGATCCAAG ATAATGGAGA ATTTTACATT 
    TCCACACACA CCTCCACTGG CTCCACAATT CAGCATACAC AAGAACTCTT AAAAGAGCAT 
    GAGGAGTTTC AGATCACAGC AAAGCAAACC AAAGAGAGGG TAAAGCTGTT GATACAGCTG 
    GCTGATGGCT TTTGTGAAAA AGGGCATGCT CATGCTGCAG AAATTAAAAA ATGTGTTACA 
    GCGGTGGACA AGAGGTACAG AGACTTCTCG CTGCGTATGG AAAAGTACAG AACCAGCTTA 
    GAGAAGGCTT TGGGCATTTC TTCTGATTCC AAGTCGAGTA AAAGCCTTCA GTTGGATATT 
    ATTCCAGCCA CTGCCCCTGG TTCCGAAGTC AAGCTTCGTG ATGCAACACA TGAACTCAAT 
    GAGGAGAAAA GAAAATCTGC TCGTCGAAAA GAATTCATCA TGGCAGAACT TATACAAACT 
    GAAAAAGCAT ATGTTCGTGA TCTTCGGGAA TGCATGGATA CATATCTGTG GGAAATGACA 
    AGCGGAGTTG AAGAGATCCC ACCAGGCATT GTAAATAAAG AACACATTAT CTTTGGAAAC 
    ATGCAGGAAA TATATGAATT TCATAACAAC ATATTCTTAA AGGAGCTTGA GAAGTATGAG 
    CAGCTACCTG AAGATGTTGG ACATTGTTTT GTCACATGGG CTGACAAATT TCAGATGTAT 
    GTGTCCTACT GTAAGAATAA GCCTGATTCA ACACAACTTA TCTTGGAACA AGCAGGAACA 
    TATTTTGATG AAATTCAGCA GCGCCATGAG CTGGTCAACT CCATCTCTTC TTATCTTATC 
    AAACCTGTGC AAAGGATAAC AAAATATCAG CTTCTTTTAA AAGAGCTGCT GACATGCTGC 
    GAAGAAGGTA AAGGAGAGAT TAAAGATGGG CTGGAAGTGA TGCTAAGTGT ACCAAAAAGA 
    GCCAACGATG CAATGCATCT CAGCATGCTT GAAGGGTTTG ATGAGAACAT TGAATCTCAG 
    GGAGAACTTA TTCTGCAAGA GTCTTTTCAA GTGTGGGATC CTAAAACCTT AATAAGAAAG 
    GGGCGTGAGA GACATCTCTT CTTGTTTGAA ATGTCTTTGG TCTTCAGCAA AGAAGTGAAA 
    GATTCAAGTG GAAGGAGCAA ATATATTTAC AAATCAAAAT TATTTACCTC AGAGCTTGGA 
    GTCACAGAAC ATGTAGAAGG TGATCCTTGC AAATTTGCAC TTTGGGTAGG CAGAACGCCA 
    ACATCTGATA ACAAAATAGT CCTTAAGGGC TCAAGTATTG AGAACAAGCA AGATTGGATC 
    AAGCATATAA GAGAAGTGAT CCAAGACCGA ACCATTCACC TGAGAGGCGC ATTAAAAGAG 
    CCTATTCATA TACCTAAAAC AGCTGCTGCT ACAAAACAGA AACCTGCCAA AAGAGATGGA 
    GAAGACCTGG ACAGTCAAGG AGATGGCAGC AGCCAACCTG ATACAATATC TATTGCTTCA 
    CGAACTTCAC AGAACACATT AGACAGTGAT AAGCTTTCTG GTGGATGTGA GCTGACAGTA 
    GTCATCCATG ACTTTACTGC CTGTAATAGC AATGAGCTGA CAATTAAACG AGGGCAGACT 
    GTGGAGGTTC TAGAGCGGCA TCACGATCGA CCTGACTGGT GTTTAGTTCG GACTACTGAC 
    CGGTCACCTG CAGCAGAGGG CTTGGTGCCT AGTAGTTCAC TGTGTATTGC ACATTCTAGA 
    AGTAGCATGG AAATGGAAGG CATATTTAAC CACAAAGATT CCCTCTCTGT CTCCAGTAAT 
    GATGCCAGTC CTCCTGCTTC AGTAATTTCC CTTCAGCCTC ACATGATTGG TGCTCAAAGC 
    TCTCCTGGCC CAAAGCGACC TGGCAATACT TTGAGAAAAT GGCTCACCAG CCCTGTCAGG 
    AGACTTAGCA GTGGCAAAGC TGATGGACAT GTGAAAAAAT TGGCTCATAA ACACAAAAAA 
    AGCAGGGAGG TTCGTAAAAA CACAGACTCA GGTTCCCAAA AGGACTCTGA TGACAGTGCA 
    GCAACACCTC AAGATGAAAC TATCGAGGAG AGAGTGCGCA ATGAAGGACT TAGCAGTGGT 
    ACGCTTTCTA AATCCTCCTC GTCTGGCATG CAAAGCTGTG GAGAAGAAGA GGGCGAGGAA 
    GGACCTGATG CAGTTCCATT GCCTCCACCC ATGGCTATTC AGCAGCACAG CCTTCTTCAG 
    CCTGACTCAC AGGATGACAA GACATCATCT CGGTTGCTGG TGCGACCTAC TAGCTCAGAG 
    ACACCTAGTG CTGCAGAGCT TGTTAGTGCT ATTGAAGAAC TTGTAAAGAG TAAAATGGCT 
    TTGGAAGACC GGCCAAGTTC ACTTCAAGTA GACCAGGGAG ACAGTAGTAG CCCATCATTC 
    AACCCTTCAG ATACATCTCT GCTTTCCTCC TCTTCTCCAA TTGATGAAAT GGAAGAAAAA 
    AAATCAAGCT CTTTAAAAAG AAGGCACTAT GTATTACAAG AGCTAGTAGA AACTGAACGC 
    GATTATGTTC GGGACCTTGG CTATGTGGTT GAGGGTTATA TGGCACTTAT GAAGGAGGAT 
    GGAGTTCCAG ATGATATGAA AGGAAAAGAC AAGATTGTTT TTGGCAACTT ACACCAGATC 
    TACGAGTGGC ACAGAGACTT TTTTTTGGGT GAACTGGAAA GGTGCCTGGA TGAGCCTGAA 
    AGATTGGCAT CCCTATTTGT CAAGCATGAA AGAAGATTGC ATATGTACAT AGTTTACTGC 
    CAAAATAAAC CAAAGTCTGA ACATATTGTT TCAGAATACA TTGATACCTA TTTTGAGGAC 
    CTGAAACAGC GTCTGGGCCA TCGACTTCAG CTTACAGATC TGTTAATAAA ACCCATACAA 
    AGAATAATGA AATATCAGCT GCTATTAAAG GATTTTCTCA AGTACTCTAA GAAGGCTTGC 
    ATAGATGTAG CAGAACTTGA GAGAGCTGTG GAAGTCATGT GTATCGTACC GAAGCGGTGT 
    AATGACATGA TGAATGTTGG TCGTTTGCAA GGATTTGATG GCAAAATAGT TGCCCAAGGA 
    AAGCTGCTAC TGCAAGACAC ATTCCTTGTG ACTGAGCAGG ACGCTGGTCT TCTGCCCCGA 
    TGCAAAGAAA GGCGGGTCTT CCTTTTCGAA CAAATTGTCA TCTTTAGTGA GCCCTTAGAT 
    AAAAAGAAAG GATACTCTAC ACCTGGATTT CTTTTTAAAA ATAATATCAA AGTGAGCTGC 
    CTTAGTTTAG AGGAGTGTGT TGATAGTGAT CCATGCAAGT TTGCACTATT GTCAAGGTCT 
    GCTGACTCTA CAGAGATGTT TGTTTTGCAT TCTTCATCGC CTGGAGTGAG ACAGTTATGG 
    ATTCATGAAA TCAACCAAAT TTTGGAAAAC CAGCGCAATT TTTTAAATGC CTTGACATCA 
    CCAATTGAAT ACCACAGAAA TCACACTGGT GGAAGCAGTA GTGGCAACGT CACCTTTACC 
    AGCAGTCCCA GCAATTGTAG TGGGCTGGGT GGCTCCAGCC GAAGCAGGCC CTCTCGGATT 
    CCCCAGCCTG TTAGACACCA CTCTCCAGTC CTGGTCTCCT CTGCAGCTTC GGCACAGTCA 
    GAGGCAGACA AGATGTCAGG TATGTCAGCT CGCCGTGCAC CAGTTGTACC TCTCAACCTC 
    TCCTTAGACA ATAACCCCAG CCAGAAAGGT AGAGTAACTG CTGCTCCAGA GACAGGCGGG 
    CGAGATAAAG AATCCAATTC AGTGCCAAAG ATGAAAATGC TTGATGGCCC CAGGAAATCA 
    GCTAGTGGCA TTCCAACCTT AGCTGATGGT GCACAGAAAG AATTGCGTGG GAGCACAGAA 
    GACAGTCGAA TTAGAAGCAC TATGGGCACT CTAACCTTAG GAAAGCCAAG AAATGGTTCT 
    GCCTCACCCC TCAACTCTCC TGTCACAATT AATTTTCCAT CGTCTTTTGG TAAGGAATCA 
    GGGCCACCAA GCAGTCCCCT TCAGAAAGGC TCTTTTTGGA GCTCAATTCC AGGATCTCCA 
    GCCAGCCGAC CTGGCTCCTT CACTTTCCCT GGAGACAGTG ACTCTCTCCA GCGACAAGTT 
    CATCGGCACT CCACACACAG CAAGGATACA GACCGCATGA GCACCTGCTC CTCGACTAGT 
    GAGCAGTCCG TTCACTCCAC TCAGAGTAAT GGGGTAAGGA CAGAGAGTGA AAGTAGCAGC 
    AGTAGCAATA TCTCCACCAT GCTGGTTACG CACGATTATA CAGCGGTGAA GGAGGATGAG 
    ATCAATGTCT ACCAGGGAGA GGTTGTACAA ATTCTGGCAA GTAACCAACA GAACATGTTT 
    CTGGTGTTCA GAGCTGCCAC TGATGAGTGC CCTGCAGCTG AGGGCTGGAT TCCAAGCTAT 
    GTTCTGGGTC ACACAAGTGC AATCATCACT GATATGACTG ATGCAACTAT GAAGAAGTCC 
    TCTTCTTGGC ATACAGCCCT TAGAATAAGA AAGAAATCTG AGAAGAGGGA AAAAGATGGC 
    AGCAGAGACA CAAAACTAGA GAATGGTTAT CGCAAATCAA GAGAAGGACT AGCTAATAAG 
    GTATCAGTTA AGCTCCTGAA TCCAAACTAC ATTTATGATG CTCCTCCAGA GTTTATTGTA 
    CCACTGAACG AGGTTACATG TGACACAGGA GAGACTGTGA TTATTCGATG CAAAGTTTGT 
    GGTCGCCCCA AGGCATCCAT TACATGGAAA GGTCCCGACC ATAATACTTT GAGCAACGAT 
    GGACACTACA GTATATCCTA CAGTGATCTT GGAGAAGCAA CTCTAAAAAT AGTTGGTGTG 
    ACCACTGAGG ATGATGGTAT ATACACCTGC ATTGCAACAA ATGACTTGGG TTCAGTATCC 
    GCTTCCTCCA ATCTTAGAGT GTTAGGTTCA TGGAGTGATG GACTGAGAGT AATCTGGAAA 
    GAAAACTTCG ACATTGCTTA TAGTGAAGTG GCTGAACTTG GCAGGGGCAG GTTTTCTGTC 
    GTGAAAAAAT GTGAACAAAG AGTTACAAGA CGCATAGTAG CCACCAAATT TGTTAACAAG 
    AAGTTGATGA AAAGAGACCA GGTTACCCAT GAACTTGGAA TTCTGCAGAG CCTTCAGCAC 
    CCACAACTGA TCAGCCTTCT TGACACTTTT GAGACACCAG CAAGCTATGT CTTAGTACTT 
    GAGATAGCTG ATCAGGGATG TTTGTTAGAA TATGTGGTAC AGTGGGGAAA CCTTACAGAA 
    GAAAAGATCA CCTCATATAT GAGAGAGGTG CTGGAGGCCC TGCAGTATCT TCATAATTGC 
    AGAATAGCAC ATCTGGACCT AAAGCCTGAG AACATTCTTG TGGATCAGAG CCTAACCAAA 
    CCAACTATAA AACTGTCAGA CTTTGGCGAT GCTGTTCAGC TTAATACCAC TTACTACATT 
    CACCAATTGT TGGGCAGTGC AGAGTTTGCC GCTCCAGAGA TCATCCTTGG GAATCCTGTC 
    TCCCTTACCT CTGATATATG GAGTATTGGA GTTCTTACTT ATGTCCTTCT CAGTGGCGTC 
    TCTCCTTTCC TTGATGAAAG TGTTGAAGAG ACATGCCTGA ACATTTGCCG ACTAGACTTT 
    AGTTTTCCCG ATGACTACTT TAAGGGAGTA AGTCAGAAGG CCAAAGAATT TGTATGTTTC 
    TTACTGCAGG GTGACCAGAC AAAGCGCCCT TCTGCTGCTG TATGTCTAAA GGAACAGTGG 
    TTGCAGCCAG GCCAGCACAA ACGTAGTGGC ACATTAGACA TGTCAAGACT GACCTCGTTT 
    ATTGAACGCC GTAAACACCA GAATGATGTT CGACCCATTG GTAGCATTAA AAACTTCCTT 
    CAGAGTCGCC TTTTACCCAG AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819810.1
    CDS73..8799
    Translation

    Target ORF information:

    RefSeq Version XM_012964356.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964356.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGTGTCTT TAGAAGGCCT TACCAAAGTA GTTGACCCTT CCCAGCTAAC TCCAGAATTT 
    GAGGGTTGCT TGGAATACAA CCATGAAGAG TGGATAGAGA TCAGAGTAGC ATTTGAGGAT 
    TTTATTAATA ATGCAACTCA TATGCTGTCT CGATTAGAGG AATTACAGGA GATGCTTGCA 
    ATTAAAGAGT TGCCTCAGGA TTTAGAGGGG GCACGCAATA TGATTGAAGA ACATTCTCAA 
    ATGAAAAAAA GGGTTATAAA GGCTCCTATT GAAGATCTTG ATTTAGAAGG GCAAAAGCTT 
    TTACAAACAA TACAAAGCAG TGATAGCTTT CCTAAGAAGA ACTCTAGTTC TGGAAATGCA 
    GACCTTCAAA ATCTTCTTCC CAAAGTTTCC ACAATGTTAG ACAGGCTTCA TTCAACACGA 
    CAACATCTTC ATCAGATGTG GCACATGCGA AAAGTAAAAC TGGACCAGTG CTTTCAGCTA 
    AGGTTGTTTG AGCAGGATGC TGAGAAGATG TTTGACTGGA TTACACACAA CAAAGGTCTG 
    TTTCTGACAA GTTATACTGA CATTGGCACC CGTAATAATC ATGCTATGGA ACTTCAGACA 
    CAGCACAATC ATTTTGCTAT GAACTGCATG AATGTCTATG TAAACATTAG CCGAATAATG 
    TCTGTGGCAA ATCGACTAGT AGAATCTGGA CACTATGCCT CACAGCAAAT TAAGCAGATA 
    GCTAACCAGT TGGAGCAAGA GTGGAAGGCT TTTGCCGCAG CCCTTGATGA ACGTAGTACG 
    TTGCTTGACA TGTCTGCAAT GTTCCATCAA AAAGCGGAGC AGTACATGGG CAATGTGGAT 
    TCATGGTGCA AAGCTTGTGG GGAAGTGGAT CTTCCATCAG AGCTGCAGGA TCTAGAAGAT 
    GCCATTCATC ACCATCAGGG AATATATGAG CATATCACTT TAGCATATTC AGAGGTTAGC 
    CAGGATGGTA AATCTTTATT AGACAAACTC CAGAGACCAT TGACTCCTGG TAGTTCTGAC 
    TCACTGACAG CTTCTGCCAA CTATTCAAAG GCTGTGCATC ACGTCTTAGA TGCAATACAT 
    GAAGTCTTGC ACCACCAGCG TCAGTTGGAA AACATCTGGC AACATCGCAA AGTTCGTCTG 
    CATCAACGCC TACAGCTCTG TGTTTTTCAG CAAGATGTTC AGCAGGTCTT GGATTGGATA 
    GAGAACCATG GTGAAGCCTT TCTCAGCAAA CACACTGGGG TAGGCAAGTC TCTGCACAGG 
    GCCAGAGCCT TGCAAAAGCG ACATGAAGAC TTTGAAGAAG TCGCTCAGAA TACATACACC 
    AATGCTGATA AGCTGCTGGA AGCTGCAGAA CAGTTGGCCC AGACAGGAGA GTGCGATCCA 
    GAGGAAATAT ACCAGGCTGC TCATCAGCTT GAGGATCGGA TTCAGGATTT TGTAAGACGT 
    GTAGAACAAA GAAAAGTTCT CTTGGACATG TCTGTATCAT TCCACTCGCA TGTTAAAGAG 
    CTATGGACAT GGCTGGAGGA CCTACAAAAG GAACTGCTGG ATGATGTCTA TGCGGAGTCA 
    GTAGAAGCTG TACAGGACTT GATCAAACGG TTTGGACAAC AGCAGCAGAC AACCCTCCAG 
    TTTACAGTAA ATGTCATCAA AGAAGGGGAG GATCTAATTC AGCAACTAAG AGATTCTGCT 
    ATATCCAGCA ACAAAACTCC TCACAACAGC TCAATCAATC ACATTGAGAC TGTCCTTCAA 
    CAACTTGATG AGGCCCAATC ACAGATGGAG GAGCTTTTCC AAGAGAGAAA AATCAAACTT 
    GAGCTATTCT TGCAGCTTCG CATCTTTGAG AGAGATGCCA TTGATATCAT CTCAGACTTG 
    GAATCATGGA ATGATGAACT TTCTCAGCAA ATGAATGATT TTGACACAGA GGACCTCACA 
    CTAGCCGAGC AAAGGCTACA GCATCACGCA GACAAAGCTC TGACAATGAA CAACCTGACA 
    TTTGATGTCA TCCACCAAGG ACAAGAGTTA CTACAATATG TGAATGAAGT TCAGAACTCA 
    GGGGTTGAGC TACTCTGTGA TAGAGATGTA GATATGGCTA CTAGAGTTCA GGACCTGTTG 
    GAATTCCTCC ATGAGAAACA ACAGGAGTTG GATCTAGCAG CTGAGCAGCA CCGAAAACAT 
    CTAGAACAAT GTGTGCAACT ACGACATTTG CAAGCTGAAG TCAAACAGGT ACTCGGCTGG 
    ATACGGAATG GAGAGTCTAT GTTAAATGCC GGGCTTATCA CTGCCAGTTC ATTGCAAGAA 
    GCAGAGCAAC TTCAAAGGGA ACATGAGCAG TTCCAGCATG CAATAGAAAA AACCCACCAG 
    AGTGCTCTGC AGGTGCAGCA GAAGGCAGAG GCCATGCTTC AAGCAAACCA TTACGACATG 
    GATATGATCC GGGACTGTGC TGAAAAAGTG GCCTCTCATT GGCAACAGTT GATGCTTAAA 
    ATGGAAGACC GTCTTAAGCT TGTCAATGCC TCTGTGGCCT TTTATAAAAC CTCAGAGCAG 
    GTATGCAGTG TTCTAGAGAG CCTAGAGCAG GAGTACAAGC GGGAGGAAGA TTGGTGTGGC 
    GGAGCTGATA AACTTGGGCC TAACTCAGAG ACTGACCATG TTACTCCTAT GATCAGCAAA 
    CACCTTGAAC AAAAGGAAGC CTTTCTTAAG GCCTGCACTT TGGCTCGCAG AAATGCTGAT 
    GTCTTTCTGA AATACTTGCA CAGGAATAGT GTCAATATGC CTGGAATGGT GACACATGTA 
    AAGGCTCCTG AACAGCAAGT AAAAAATATT TTAAATGAGC TGTTTCAGAG GGAAAATAGA 
    GTTCTTCATT ATTGGACCTT GCGGAAAAGG AGACTAGACC ATTGCCAGCA GTATGTAGTA 
    TTTGAAAGAA GTGCCAAACA GGCTCTGGAG TGGATCCAAG ATAATGGAGA ATTTTACATT 
    TCCACACACA CCTCCACTGG CTCCACAATT CAGCATACAC AAGAACTCTT AAAAGAGCAT 
    GAGGAGTTTC AGATCACAGC AAAGCAAACC AAAGAGAGGG TAAAGCTGTT GATACAGCTG 
    GCTGATGGCT TTTGTGAAAA AGGGCATGCT CATGCTGCAG AAATTAAAAA ATGTGTTACA 
    GCGGTGGACA AGAGGTACAG AGACTTCTCG CTGCGTATGG AAAAGTACAG AACCAGCTTA 
    GAGAAGGCTT TGGGCATTTC TTCTGATTCC AAGTCGAGTA AAAGCCTTCA GTTGGATATT 
    ATTCCAGCCA CTGCCCCTGG TTCCGAAGTC AAGCTTCGTG ATGCAACACA TGAACTCAAT 
    GAGGAGAAAA GAAAATCTGC TCGTCGAAAA GAATTCATCA TGGCAGAACT TATACAAACT 
    GAAAAAGCAT ATGTTCGTGA TCTTCGGGAA TGCATGGATA CATATCTGTG GGAAATGACA 
    AGCGGAGTTG AAGAGATCCC ACCAGGCATT GTAAATAAAG AACACATTAT CTTTGGAAAC 
    ATGCAGGAAA TATATGAATT TCATAACAAC ATATTCTTAA AGGAGCTTGA GAAGTATGAG 
    CAGCTACCTG AAGATGTTGG ACATTGTTTT GTCACATGGG CTGACAAATT TCAGATGTAT 
    GTGTCCTACT GTAAGAATAA GCCTGATTCA ACACAACTTA TCTTGGAACA AGCAGGAACA 
    TATTTTGATG AAATTCAGCA GCGCCATGAG CTGGTCAACT CCATCTCTTC TTATCTTATC 
    AAACCTGTGC AAAGGATAAC AAAATATCAG CTTCTTTTAA AAGAGCTGCT GACATGCTGC 
    GAAGAAGGTA AAGGAGAGAT TAAAGATGGG CTGGAAGTGA TGCTAAGTGT ACCAAAAAGA 
    GCCAACGATG CAATGCATCT CAGCATGCTT GAAGGGTTTG ATGAGAACAT TGAATCTCAG 
    GGAGAACTTA TTCTGCAAGA GTCTTTTCAA GTGTGGGATC CTAAAACCTT AATAAGAAAG 
    GGGCGTGAGA GACATCTCTT CTTGTTTGAA ATGTCTTTGG TCTTCAGCAA AGAAGTGAAA 
    GATTCAAGTG GAAGGAGCAA ATATATTTAC AAATCAAAAT TATTTACCTC AGAGCTTGGA 
    GTCACAGAAC ATGTAGAAGG TGATCCTTGC AAATTTGCAC TTTGGGTAGG CAGAACGCCA 
    ACATCTGATA ACAAAATAGT CCTTAAGGGC TCAAGTATTG AGAACAAGCA AGATTGGATC 
    AAGCATATAA GAGAAGTGAT CCAAGACCGA ACCATTCACC TGAGAGGCGC ATTAAAAGAG 
    CCTATTCATA TACCTAAAAC AGCTGCTGCT ACAAAACAGA AACCTGCCAA AAGAGATGGA 
    GAAGACCTGG ACAGTCAAGG AGATGGCAGC AGCCAACCTG ATACAATATC TATTGCTTCA 
    CGAACTTCAC AGAACACATT AGACAGTGAT AAGCTTTCTG GTGGATGTGA GCTGACAGTA 
    GTCATCCATG ACTTTACTGC CTGTAATAGC AATGAGCTGA CAATTAAACG AGGGCAGACT 
    GTGGAGGTTC TAGAGCGGCA TCACGATCGA CCTGACTGGT GTTTAGTTCG GACTACTGAC 
    CGGTCACCTG CAGCAGAGGG CTTGGTGCCT AGTAGTTCAC TGTGTATTGC ACATTCTAGA 
    AGTAGCATGG AAATGGAAGG CATATTTAAC CACAAAGATT CCCTCTCTGT CTCCAGTAAT 
    GATGCCAGTC CTCCTGCTTC AGTAATTTCC CTTCAGCCTC ACATGATTGG TGCTCAAAGC 
    TCTCCTGGCC CAAAGCGACC TGGCAATACT TTGAGAAAAT GGCTCACCAG CCCTGTCAGG 
    AGACTTAGCA GTGGCAAAGC TGATGGACAT GTGAAAAAAT TGGCTCATAA ACACAAAAAA 
    AGCAGGGAGG TTCGTAAAAA CACAGACTCA GGTTCCCAAA AGGACTCTGA TGACAGTGCA 
    GCAACACCTC AAGATGAAAC TATCGAGGAG AGAGTGCGCA ATGAAGGACT TAGCAGTGGT 
    ACGCTTTCTA AATCCTCCTC GTCTGGCATG CAAAGCTGTG GAGAAGAAGA GGGCGAGGAA 
    GGACCTGATG CAGTTCCATT GCCTCCACCC ATGGCTATTC AGCAGCACAG CCTTCTTCAG 
    CCTGACTCAC AGGATGACAA GACATCATCT CGGTTGCTGG TGCGACCTAC TAGCTCAGAG 
    ACACCTAGTG CTGCAGAGCT TGTTAGTGCT ATTGAAGAAC TTGTAAAGAG TAAAATGGCT 
    TTGGAAGACC GGCCAAGTTC ACTTCAAGTA GACCAGGGAG ACAGTAGTAG CCCATCATTC 
    AACCCTTCAG ATACATCTCT GCTTTCCTCC TCTTCTCCAA TTGATGAAAT GGAAGAAAAA 
    AAATCAAGCT CTTTAAAAAG AAGGCACTAT GTATTACAAG AGCTAGTAGA AACTGAACGC 
    GATTATGTTC GGGACCTTGG CTATGTGGTT GAGGGTTATA TGGCACTTAT GAAGGAGGAT 
    GGAGTTCCAG ATGATATGAA AGGAAAAGAC AAGATTGTTT TTGGCAACTT ACACCAGATC 
    TACGAGTGGC ACAGAGACTT TTTTTTGGGT GAACTGGAAA GGTGCCTGGA TGAGCCTGAA 
    AGATTGGCAT CCCTATTTGT CAAGCATGAA AGAAGATTGC ATATGTACAT AGTTTACTGC 
    CAAAATAAAC CAAAGTCTGA ACATATTGTT TCAGAATACA TTGATACCTA TTTTGAGGAC 
    CTGAAACAGC GTCTGGGCCA TCGACTTCAG CTTACAGATC TGTTAATAAA ACCCATACAA 
    AGAATAATGA AATATCAGCT GCTATTAAAG GATTTTCTCA AGTACTCTAA GAAGGCTTGC 
    ATAGATGTAG CAGAACTTGA GAGAGCTGTG GAAGTCATGT GTATCGTACC GAAGCGGTGT 
    AATGACATGA TGAATGTTGG TCGTTTGCAA GGATTTGATG GCAAAATAGT TGCCCAAGGA 
    AAGCTGCTAC TGCAAGACAC ATTCCTTGTG ACTGAGCAGG ACGCTGGTCT TCTGCCCCGA 
    TGCAAAGAAA GGCGGGTCTT CCTTTTCGAA CAAATTGTCA TCTTTAGTGA GCCCTTAGAT 
    AAAAAGAAAG GATACTCTAC ACCTGGATTT CTTTTTAAAA ATAATATCAA AGTGAGCTGC 
    CTTAGTTTAG AGGAGTGTGT TGATAGTGAT CCATGCAAGT TTGCACTATT GTCAAGGTCT 
    GCTGACTCTA CAGAGATGTT TGTTTTGCAT TCTTCATCGC CTGGAGTGAG ACAGTTATGG 
    ATTCATGAAA TCAACCAAAT TTTGGAAAAC CAGCGCAATT TTTTAAATGC CTTGACATCA 
    CCAATTGAAT ACCACAGAAA TCACACTGGT GGAAGCAGTA GTGGCAACGT CACCTTTACC 
    AGCAGTCCCA GCAATTGTAG TGGGCTGGGT GGCTCCAGCC GAAGCAGGCC CTCTCGGATT 
    CCCCAGCCTG TTAGACACCA CTCTCCAGTC CTGGTCTCCT CTGCAGCTTC GGCACAGTCA 
    GAGGCAGACA AGATGTCAGG TATGTCAGCT CGCCGTGCAC CAGTTGTACC TCTCAACCTC 
    TCCTTAGACA ATAACCCCAG CCAGAAAGGT AGAGTAACTG CTGCTCCAGA GACAGGCGGG 
    CGAGATAAAG AATCCAATTC AGTGCCAAAG ATGAAAATGC TTGATGGCCC CAGGAAATCA 
    GCTAGTGGCA TTCCAACCTT AGCTGATGGT GCACAGAAAG AATTGCGTGG GAGCACAGAA 
    GACAGTCGAA TTAGAAGCAC TATGGGCACT CTAACCTTAG GAAAGCCAAG AAATGGTTCT 
    GCCTCACCCC TCAACTCTCC TGTCACAATT AATTTTCCAT CGTCTTTTGG TAAGGAATCA 
    GGGCCACCAA GCAGTCCCCT TCAGAAAGGC TCTTTTTGGA GCTCAATTCC AGGATCTCCA 
    GCCAGCCGAC CTGGCTCCTT CACTTTCCCT GGAGACAGTG ACTCTCTCCA GCGACAAGTT 
    CATCGGCACT CCACACACAG CAAGGATACA GACCGCATGA GCACCTGCTC CTCGACTAGT 
    GAGCAGTCCG TTCACTCCAC TCAGAGTAAT GGGGTAAGGA CAGAGAGTGA AAGTAGCAGC 
    AGTAGCAATA TCTCCACCAT GCTGGTTACG CACGATTATA CAGCGGTGAA GGAGGATGAG 
    ATCAATGTCT ACCAGGGAGA GGTTGTACAA ATTCTGGCAA GTAACCAACA GAACATGTTT 
    CTGGTGTTCA GAGCTGCCAC TGATGAGTGC CCTGCAGCTG AGGGCTGGAT TCCAAGCTAT 
    GTTCTGGGTC ACACAAGTGC AATCATCACT GATATGACTG ATGCAACTAT GAAGAAGTCC 
    TCTTCTTGGC ATACAGCCCT TAGAATAAGA AAGAAATCTG AGAAGAGGGA AAAAGATGGC 
    AGCAGAGACA CAAAACTAGA GAATGGTTAT CGCAAATCAA GAGAAGGACT AGCTAATAAG 
    GTATCAGTTA AGCTCCTGAA TCCAAACTAC ATTTATGATG CTCCTCCAGA GTTTATTGTA 
    CCACTGAACG AGGTTACATG TGACACAGGA GAGACTGTGA TTATTCGATG CAAAGTTTGT 
    GGTCGCCCCA AGGCATCCAT TACATGGAAA GGTCCCGACC ATAATACTTT GAGCAACGAT 
    GGACACTACA GTATATCCTA CAGTGATCTT GGAGAAGCAA CTCTAAAAAT AGTTGGTGTG 
    ACCACTGAGG ATGATGGTAT ATACACCTGC ATTGCAACAA ATGACTTGGG TTCAGTATCC 
    GCTTCCTCCA ATCTTAGAGT GTTAGGTTCA TGGAGTGATG GACTGAGAGT AATCTGGAAA 
    GAAAACTTCG ACATTGCTTA TAGTGAAGTG GCTGAACTTG GCAGGGGCAG GTTTTCTGTC 
    GTGAAAAAAT GTGAACAAAG AGTTACAAGA CGCATAGTAG CCACCAAATT TGTTAACAAG 
    AAGTTGATGA AAAGAGACCA GGTTACCCAT GAACTTGGAA TTCTGCAGAG CCTTCAGCAC 
    CCACAACTGA TCAGCCTTCT TGACACTTTT GAGACACCAG CAAGCTATGT CTTAGTACTT 
    GAGATAGCTG ATCAGGGATG TTTGTTAGAA TATGTGGTAC AGTGGGGAAA CCTTACAGAA 
    GAAAAGATCA CCTCATATAT GAGAGAGGTG CTGGAGGCCC TGCAGTATCT TCATAATTGC 
    AGAATAGCAC ATCTGGACCT AAAGCCTGAG AACATTCTTG TGGATCAGAG CCTAACCAAA 
    CCAACTATAA AACTGTCAGA CTTTGGCGAT GCTGTTCAGC TTAATACCAC TTACTACATT 
    CACCAATTGT TGGGCAGTGC AGAGTTTGCC GCTCCAGAGA TCATCCTTGG GAATCCTGTC 
    TCCCTTACCT CTGATATATG GAGTATTGGA GTTCTTACTT ATGTCCTTCT CAGTGGCGTC 
    TCTCCTTTCC TTGATGAAAG TGTTGAAGAG ACATGCCTGA ACATTTGCCG ACTAGACTTT 
    AGTTTTCCCG ATGACTACTT TAAGGGAGTA AGTCAGAAGG CCAAAGAATT TGTATGTTTC 
    TTACTGCAGG GTGACCAGAC AAAGCGCCCT TCTGCTGCTG TATGTCTAAA GGAACAGTGG 
    TTGCAGCCAG GCCAGCACAA ACGTAGTGGC ACATTAGACA TGTCAAGACT GACCTCGTTT 
    ATTGAACGCC GTAAACACCA GAATGATGTT CGACCCATTG GTAGCATTAA AAACTTCCTT 
    CAGAGTCGCC TTTTACCCAG AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_018094560.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950049.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OXa44862
    Clone ID Related Accession (Same CDS sequence) XM_018094560.1
    Accession Version XM_018094560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8607bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT 
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC 
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC 
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC 
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG 
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC 
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC 
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA 
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG 
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG 
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATCCTG 
    AGAACATTCT TGTGGATCAG AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950049.1
    CDS619..9225
    Translation

    Target ORF information:

    RefSeq Version XM_018094560.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_018094560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGAGCACAT CCAGCACCGC AAGCTCCGGT AGCGCAAATA CCGGTACCGG CAATGCCGTT 
    GGAAGCAGCG GAGGCAACAC CGGGGAGGGG GCGGAGGAGA CGGCCAAGGA CTCTGCAGAC 
    ATAGCTGCCT TTTACAGATC CGGTTTTCGT AGAAATGATG AATTGAAAGC AATGGAGGTG 
    TTGCCAATTT TAAAAGAAAA AGTGGCATAT CTATCAGGTG GCAGAGATAA ACGAGGTGGT 
    CCTGTATTAA CATTTCCAGC ACGCAGCAAC CATGATCGAA TACGACAAGA GGATCTCCGA 
    AGACTGATTT CATATCTTGC TTGTATTCCT AGTGAGGAGG TGTGCAAGCG AGGCTTCACA 
    GTGATTGTAG ATATGCGTGG TTCCAAGTGG GATTCTATTA AACCCTTGCT GAAAATTTTA 
    CAAGAATCCT TTCCATGCTG TATTCATGTT GCACTAATTA TCAAACCAGA TAACTTCTGG 
    CAGAAGCAAA GAACTAATTT TGGAAGTTCT AAGTTTGAAT TTGAGACAAG TATGGTGTCT 
    TTAGAAGGCC TTACCAAAGT AGTTGACCCT TCCCAGCTAA CTCCAGAATT TGAGGGTTGC 
    TTGGAATACA ACCATGAAGA GTGGATAGAG ATCAGAGTAG CATTTGAGGA TTTTATTAAT 
    AATGCAACTC ATATGCTGTC TCGATTAGAG GAATTACAGG AGATGCTTGC AATTAAAGAG 
    TTGCCTCAGG ATTTAGAGGG GGCACGCAAT ATGATTGAAG AACATTCTCA AATGAAAAAA 
    AGGGTTATAA AGGCTCCTAT TGAAGATCTT GATTTAGAAG GGCAAAAGCT TTTACAAACA 
    ATACAAAGCA GTGATAGCTT TCCTAAGAAG AACTCTAGTT CTGGAAATGC AGACCTTCAA 
    AATCTTCTTC CCAAAGTTTC CACAATGTTA GACAGGCTTC ATTCAACACG ACAACATCTT 
    CATCAGATGT GGCACATGCG AAAAGTAAAA CTGGACCAGT GCTTTCAGCT AAGGTTGTTT 
    GAGCAGGATG CTGAGAAGAT GTTTGACTGG ATTACACACA ACAAAGGTCT GTTTCTGACA 
    AGTTATACTG ACATTGGCAC CCGTAATAAT CATGCTATGG AACTTCAGAC ACAGCACAAT 
    CATTTTGCTA TGAACTGCAT GAATGTCTAT GTAAACATTA GCCGAATAAT GTCTGTGGCA 
    AATCGACTAG TAGAATCTGG ACACTATGCC TCACAGCAAA TTAAGCAGAT AGCTAACCAG 
    TTGGAGCAAG AGTGGAAGGC TTTTGCCGCA GCCCTTGATG AACGTAGTAC GTTGCTTGAC 
    ATGTCTGCAA TGTTCCATCA AAAAGCGGAG CAGTACATGG GCAATGTGGA TTCATGGTGC 
    AAAGCTTGTG GGGAAGTGGA TCTTCCATCA GAGCTGCAGG ATCTAGAAGA TGCCATTCAT 
    CACCATCAGG GAATATATGA GCATATCACT TTAGCATATT CAGAGGTTAG CCAGGATGGT 
    AAATCTTTAT TAGACAAACT CCAGAGACCA TTGACTCCTG GTAGTTCTGA CTCACTGACA 
    GCTTCTGCCA ACTATTCAAA GGCTGTGCAT CACGTCTTAG ATGCAATACA TGAAGTCTTG 
    CACCACCAGC GTCAGTTGGA AAACATCTGG CAACATCGCA AAGTTCGTCT GCATCAACGC 
    CTACAGCTCT GTGTTTTTCA GCAAGATGTT CAGCAGGTCT TGGATTGGAT AGAGAACCAT 
    GGTGAAGCCT TTCTCAGCAA ACACACTGGG GTAGGCAAGT CTCTGCACAG GGCCAGAGCC 
    TTGCAAAAGC GACATGAAGA CTTTGAAGAA GTCGCTCAGA ATACATACAC CAATGCTGAT 
    AAGCTGCTGG AAGCTGCAGA ACAGTTGGCC CAGACAGGAG AGTGCGATCC AGAGGAAATA 
    TACCAGGCTG CTCATCAGCT TGAGGATCGG ATTCAGGATT TTGTAAGACG TGTAGAACAA 
    AGAAAAGTTC TCTTGGACAT GTCTGTATCA TTCCACTCGC ATGTTAAAGA GCTATGGACA 
    TGGCTGGAGG ACCTACAAAA GGAACTGCTG GATGATGTCT ATGCGGAGTC AGTAGAAGCT 
    GTACAGGACT TGATCAAACG GTTTGGACAA CAGCAGCAGA CAACCCTCCA GTTTACAGTA 
    AATGTCATCA AAGAAGGGGA GGATCTAATT CAGCAACTAA GAGATTCTGC TATATCCAGC 
    AACAAAACTC CTCACAACAG CTCAATCAAT CACATTGAGA CTGTCCTTCA ACAACTTGAT 
    GAGGCCCAAT CACAGATGGA GGAGCTTTTC CAAGAGAGAA AAATCAAACT TGAGCTATTC 
    TTGCAGCTTC GCATCTTTGA GAGAGATGCC ATTGATATCA TCTCAGACTT GGAATCATGG 
    AATGATGAAC TTTCTCAGCA AATGAATGAT TTTGACACAG AGGACCTCAC ACTAGCCGAG 
    CAAAGGCTAC AGCATCACGC AGACAAAGCT CTGACAATGA ACAACCTGAC ATTTGATGTC 
    ATCCACCAAG GACAAGAGTT ACTACAATAT GTGAATGAAG TTCAGAACTC AGGGGTTGAG 
    CTACTCTGTG ATAGAGATGT AGATATGGCT ACTAGAGTTC AGGACCTGTT GGAATTCCTC 
    CATGAGAAAC AACAGGAGTT GGATCTAGCA GCTGAGCAGC ACCGAAAACA TCTAGAACAA 
    TGTGTGCAAC TACGACATTT GCAAGCTGAA GTCAAACAGG TACTCGGCTG GATACGGAAT 
    GGAGAGTCTA TGTTAAATGC CGGGCTTATC ACTGCCAGTT CATTGCAAGA AGCAGAGCAA 
    CTTCAAAGGG AACATGAGCA GTTCCAGCAT GCAATAGAAA AAACCCACCA GAGTGCTCTG 
    CAGGTGCAGC AGAAGGCAGA GGCCATGCTT CAAGCAAACC ATTACGACAT GGATATGATC 
    CGGGACTGTG CTGAAAAAGT GGCCTCTCAT TGGCAACAGT TGATGCTTAA AATGGAAGAC 
    CGTCTTAAGC TTGTCAATGC CTCTGTGGCC TTTTATAAAA CCTCAGAGCA GGTATGCAGT 
    GTTCTAGAGA GCCTAGAGCA GGAGTACAAG CGGGAGGAAG ATTGGTGTGG CGGAGCTGAT 
    AAACTTGGGC CTAACTCAGA GACTGACCAT GTTACTCCTA TGATCAGCAA ACACCTTGAA 
    CAAAAGGAAG CCTTTCTTAA GGCCTGCACT TTGGCTCGCA GAAATGCTGA TGTCTTTCTG 
    AAATACTTGC ACAGGAATAG TGTCAATATG CCTGGAATGG TGACACATGT AAAGGCTCCT 
    GAACAGCAAG TAAAAAATAT TTTAAATGAG CTGTTTCAGA GGGAAAATAG AGTTCTTCAT 
    TATTGGACCT TGCGGAAAAG GAGACTAGAC CATTGCCAGC AGTATGTAGT ATTTGAAAGA 
    AGTGCCAAAC AGGCTCTGGA GTGGATCCAA GATAATGGAG AATTTTACAT TTCCACACAC 
    ACCTCCACTG GCTCCACAAT TCAGCATACA CAAGAACTCT TAAAAGAGCA TGAGGAGTTT 
    CAGATCACAG CAAAGCAAAC CAAAGAGAGG GTAAAGCTGT TGATACAGCT GGCTGATGGC 
    TTTTGTGAAA AAGGGCATGC TCATGCTGCA GAAATTAAAA AATGTGTTAC AGCGGTGGAC 
    AAGAGGTACA GAGACTTCTC GCTGCGTATG GAAAAGTACA GAACCAGCTT AGAGAAGGCT 
    TTGGGCATTT CTTCTGATTC CAAGTCGAGT AAAAGCCTTC AGTTGGATAT TATTCCAGCC 
    ACTGCCCCTG GTTCCGAAGT CAAGCTTCGT GATGCAACAC ATGAACTCAA TGAGGAGAAA 
    AGAAAATCTG CTCGTCGAAA AGAATTCATC ATGGCAGAAC TTATACAAAC TGAAAAAGCA 
    TATGTTCGTG ATCTTCGGGA ATGCATGGAT ACATATCTGT GGGAAATGAC AAGCGGAGTT 
    GAAGAGATCC CACCAGGCAT TGTAAATAAA GAACACATTA TCTTTGGAAA CATGCAGGAA 
    ATATATGAAT TTCATAACAA CATATTCTTA AAGGAGCTTG AGAAGTATGA GCAGCTACCT 
    GAAGATGTTG GACATTGTTT TGTCACATGG GCTGACAAAT TTCAGATGTA TGTGTCCTAC 
    TGTAAGAATA AGCCTGATTC AACACAACTT ATCTTGGAAC AAGCAGGAAC ATATTTTGAT 
    GAAATTCAGC AGCGCCATGA GCTGGTCAAC TCCATCTCTT CTTATCTTAT CAAACCTGTG 
    CAAAGGATAA CAAAATATCA GCTTCTTTTA AAAGAGCTGC TGACATGCTG CGAAGAAGGT 
    AAAGGAGAGA TTAAAGATGG GCTGGAAGTG ATGCTAAGTG TACCAAAAAG AGCCAACGAT 
    GCAATGCATC TCAGCATGCT TGAAGGGTTT GATGAGAACA TTGAATCTCA GGGAGAACTT 
    ATTCTGCAAG AGTCTTTTCA AGTGTGGGAT CCTAAAACCT TAATAAGAAA GGGGCGTGAG 
    AGACATCTCT TCTTGTTTGA AATGTCTTTG GTCTTCAGCA AAGAAGTGAA AGATTCAAGT 
    GGAAGGAGCA AATATATTTA CAAATCAAAA TTATTTACCT CAGAGCTTGG AGTCACAGAA 
    CATGTAGAAG GTGATCCTTG CAAATTTGCA CTTTGGGTAG GCAGAACGCC AACATCTGAT 
    AACAAAATAG TCCTTAAGGG CTCAAGTATT GAGAACAAGC AAGATTGGAT CAAGCATATA 
    AGAGAAGTGA TCCAAGACCG AACCATTCAC CTGAGAGGCG CATTAAAAGA GCCTATTCAT 
    ATACCTAAAA CAGCTGCTGC TACAAAACAG AAACCTGCCA AAAGAGATGG AGAAGACCTG 
    GACAGTCAAG GAGATGGCAG CAGCCAACCT GATACAATAT CTATTGCTTC ACGAACTTCA 
    CAGAACACAT TAGACAGTGA TAAGCTTTCT GGTGGATGTG AGCTGACAGT AGTCATCCAT 
    GACTTTACTG CCTGTAATAG CAATGAGCTG ACAATTAAAC GAGGGCAGAC TGTGGAGGTT 
    CTAGAGCGGC ATCACGATCG ACCTGACTGG TGTTTAGTTC GGACTACTGA CCGGTCACCT 
    GCAGCAGAGG GCTTGGTGCC TAGTAGTTCA CTGTGTATTG CACATTCTAG AAGTAGCATG 
    GAAATGGAAG GCATATTTAA CCACAAAGAT TCCCTCTCTG TCTCCAGTAA TGATGCCAGT 
    CCTCCTGCTT CAGTAATTTC CCTTCAGCCT CACATGATTG GTGCTCAAAG CTCTCCTGGC 
    CCAAAGCGAC CTGGCAATAC TTTGAGAAAA TGGCTCACCA GCCCTGTCAG GAGACTTAGC 
    AGTGGCAAAG CTGATGGACA TGTGAAAAAA TTGGCTCATA AACACAAAAA AAGCAGGGAG 
    GTTCGTAAAA ACACAGACTC AGGTTCCCAA AAGGACTCTG ATGACAGTGC AGCAACACCT 
    CAAGATGAAA CTATCGAGGA GAGAGTGCGC AATGAAGGAC TTAGCAGTGG TACGCTTTCT 
    AAATCCTCCT CGTCTGGCAT GCAAAGCTGT GGAGAAGAAG AGGGCGAGGA AGGACCTGAT 
    GCAGTTCCAT TGCCTCCACC CATGGCTATT CAGCAGCACA GCCTTCTTCA GCCTGACTCA 
    CAGGATGACA AGACATCATC TCGGTTGCTG GTGCGACCTA CTAGCTCAGA GACACCTAGT 
    GCTGCAGAGC TTGTTAGTGC TATTGAAGAA CTTGTAAAGA GTAAAATGGC TTTGGAAGAC 
    CGGCCAAGTT CACTTCAAGT AGACCAGGGA GACAGTAGTA GCCCATCATT CAACCCTTCA 
    GATACATCTC TGCTTTCCTC CTCTTCTCCA ATTGATGAAA TGGAAGAAAA AAAATCAAGC 
    TCTTTAAAAA GAAGGCACTA TGTATTACAA GAGCTAGTAG AAACTGAACG CGATTATGTT 
    CGGGACCTTG GCTATGTGGT TGAGGGTTAT ATGGCACTTA TGAAGGAGGA TGGAGTTCCA 
    GATGATATGA AAGGAAAAGA CAAGATTGTT TTTGGCAACT TACACCAGAT CTACGAGTGG 
    CACAGAGACT TTTTTTTGGG TGAACTGGAA AGGTGCCTGG ATGAGCCTGA AAGATTGGCA 
    TCCCTATTTG TCAAGCATGA AAGAAGATTG CATATGTACA TAGTTTACTG CCAAAATAAA 
    CCAAAGTCTG AACATATTGT TTCAGAATAC ATTGATACCT ATTTTGAGGA CCTGAAACAG 
    CGTCTGGGCC ATCGACTTCA GCTTACAGAT CTGTTAATAA AACCCATACA AAGAATAATG 
    AAATATCAGC TGCTATTAAA GGATTTTCTC AAGTACTCTA AGAAGGCTTG CATAGATGTA 
    GCAGAACTTG AGAGAGCTGT GGAAGTCATG TGTATCGTAC CGAAGCGGTG TAATGACATG 
    ATGAATGTTG GTCGTTTGCA AGGATTTGAT GGCAAAATAG TTGCCCAAGG AAAGCTGCTA 
    CTGCAAGACA CATTCCTTGT GACTGAGCAG GACGCTGGTC TTCTGCCCCG ATGCAAAGAA 
    AGGCGGGTCT TCCTTTTCGA ACAAATTGTC ATCTTTAGTG AGCCCTTAGA TAAAAAGAAA 
    GGATACTCTA CACCTGGATT TCTTTTTAAA AATAATATCA AAGTGAGCTG CCTTAGTTTA 
    GAGGAGTGTG TTGATAGTGA TCCATGCAAG TTTGCACTAT TGTCAAGGTC TGCTGACTCT 
    ACAGAGATGT TTGTTTTGCA TTCTTCATCG CCTGGAGTGA GACAGTTATG GATTCATGAA 
    ATCAACCAAA TTTTGGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC ACCAATTGAA 
    TACCACAGAA ATCACACTGG TGGAAGCAGT AGTGGCAACG TCACCTTTAC CAGCAGTCCC 
    AGCAATTGTA GTGGGCTGGG TGGCTCCAGC CGAAGCAGGC CCTCTCGGAT TCCCCAGCCT 
    GTTAGACACC ACTCTCCAGT CCTGGTCTCC TCTGCAGCTT CGGCACAGTC AGAGGCAGAC 
    AAGATGTCAG GTATGTCAGC TCGCCGTGCA CCAGTTGTAC CTCTCAACCT CTCCTTAGAC 
    AATAACCCCA GCCAGAAAGG TAGAGTAACT GCTGCTCCAG AGACAGGCGG GCGAGATAAA 
    GAATCCAATT CAGTGCCAAA GATGAAAATG CTTGATGGCC CCAGGAAATC AGCTAGTGGC 
    ATTCCAACCT TAGCTGATGG TGCACAGAAA GAATTGCGTG GGAGCACAGA AGACAGTCGA 
    ATTAGAAGCA CTATGGGCAC TCTAACCTTA GGAAAGCCAA GAAATGGTTC TGCCTCACCC 
    CTCAACTCTC CTGTCACAAT TAATTTTCCA TCGTCTTTTG GTAAGGAATC AGGGCCACCA 
    AGCAGTCCCC TTCAGAAAGG CTCTTTTTGG AGCTCAATTC CAGGATCTCC AGCCAGCCGA 
    CCTGGCTCCT TCACTTTCCC TGGAGACAGT GACTCTCTCC AGCGACAAGT TCATCGGCAC 
    TCCACACACA GCAAGGATAC AGACCGCATG AGCACCTGCT CCTCGACTAG TGAGCAGTCC 
    GTTCACTCCA CTCAGAGTAA TGGGGTAAGG ACAGAGAGTG AAAGTAGCAG CAGTAGCAAT 
    ATCTCCACCA TGCTGGTTAC GCACGATTAT ACAGCGGTGA AGGAGGATGA GATCAATGTC 
    TACCAGGGAG AGGTTGTACA AATTCTGGCA AGTAACCAAC AGAACATGTT TCTGGTGTTC 
    AGAGCTGCCA CTGATGAGTG CCCTGCAGCT GAGGGCTGGA TTCCAAGCTA TGTTCTGGGT 
    CACACAAGTG CAATCATCAC TGATATGACT GATGCAACTA TGAAGAAGTC CTCTTCTTGG 
    CATACAGCCC TTAGAATAAG AAAGAAATCT GAGAAGAGGG AAAAAGATGG CAGCAGAGAC 
    ACAAAACTAG AGAATGGTTA TCGCAAATCA AGAGAAGGAC TAGCTAATAA GGTATCAGTT 
    AAGCTCCTGA ATCCAAACTA CATTTATGAT GCTCCTCCAG AGTTTATTGT ACCACTGAAC 
    GAGGTTACAT GTGACACAGG AGAGACTGTG ATTATTCGAT GCAAAGTTTG TGGTCGCCCC 
    AAGGCATCCA TTACATGGAA AGGTCCCGAC CATAATACTT TGAGCAACGA TGGACACTAC 
    AGTATATCCT ACAGTGATCT TGGAGAAGCA ACTCTAAAAA TAGTTGGTGT GACCACTGAG 
    GATGATGGTA TATACACCTG CATTGCAACA AATGACTTGG GTTCAGTATC CGCTTCCTCC 
    AATCTTAGAG TGTTAGGTTC ATGGAGTGAT GGACTGAGAG TAATCTGGAA AGAAAACTTC 
    GACATTGCTT ATAGTGAAGT GGCTGAACTT GGCAGGGGCA GGTTTTCTGT CGTGAAAAAA 
    TGTGAACAAA GAGTTACAAG ACGCATAGTA GCCACCAAAT TTGTTAACAA GAAGTTGATG 
    AAAAGAGACC AGGTTACCCA TGAACTTGGA ATTCTGCAGA GCCTTCAGCA CCCACAACTG 
    ATCAGCCTTC TTGACACTTT TGAGACACCA GCAAGCTATG TCTTAGTACT TGAGATCCTG 
    AGAACATTCT TGTGGATCAG AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    trio ( XM_031903241.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759101.1 Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OXa59535
    Clone ID Related Accession (Same CDS sequence) XM_031903241.1
    Accession Version XM_031903241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product triple functional domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGCAGACTC ATCGCTGGAA AGATGACGGA GAAACAACAT TAGGTTTTCG TAGAAATGAT 
    GAATTGAAAG CAATGGAGGT GTTGCCAATT TTAAAAGAAA AAGTGGCATA TCTATCAGGT 
    GGCAGAGATA AACGAGGTGG TCCTGTATTA ACATTTCCAG CACGCAGCAA CCATGATCGA 
    ATACGACAAG AGGATCTCCG AAGACTGATT TCATATCTTG CTTGTATTCC TAGTGAGGAG 
    GTGTGCAAGC GAGGCTTCAC AGTGATTGTA GATATGCGTG GTTCCAAGTG GGATTCTATT 
    AAACCCTTGC TGAAAATTTT ACAAGAATCC TTTCCATGCT GTATTCATGT TGCACTAATT 
    ATCAAACCAG ATAACTTCTG GCAGAAGCAA AGAACTAATT TTGGAAGTTC TAAGTTTGAA 
    TTTGAGACAA GTATGGTGTC TTTAGAAGGC CTTACCAAAG TAGTTGACCC TTCCCAGCTA 
    ACTCCAGAAT TTGAGGGTTG CTTGGAATAC AACCATGAAG AGTGGATAGA GATCAGAGTA 
    GCATTTGAGG ATTTTATTAA TAATGCAACT CATATGCTGT CTCGATTAGA GGAATTACAG 
    GAGATGCTTG CAATTAAAGA GTTGCCTCAG GATTTAGAGG GGGCACGCAA TATGATTGAA 
    GAACATTCTC AAATGAAAAA AAGGGTTATA AAGGCTCCTA TTGAAGATCT TGATTTAGAA 
    GGGCAAAAGC TTTTACAAAC AATACAAAGC AGTGATAGCT TTCCTAAGAA GAACTCTAGT 
    TCTGGAAATG CAGACCTTCA AAATCTTCTT CCCAAAGTTT CCACAATGTT AGACAGGCTT 
    CATTCAACAC GACAACATCT TCATCAGATG TGGCACATGC GAAAAGTAAA ACTGGACCAG 
    TGCTTTCAGC TAAGGTTGTT TGAGCAGGAT GCTGAGAAGA TGTTTGACTG GATTACACAC 
    AACAAAGGTC TGTTTCTGAC AAGTTATACT GACATTGGCA CCCGTAATAA TCATGCTATG 
    GAACTTCAGA CACAGCACAA TCATTTTGCT ATGAACTGCA TGAATGTCTA TGTAAACATT 
    AGCCGAATAA TGTCTGTGGC AAATCGACTA GTAGAATCTG GACACTATGC CTCACAGCAA 
    ATTAAGCAGA TAGCTAACCA GTTGGAGCAA GAGTGGAAGG CTTTTGCCGC AGCCCTTGAT 
    GAACGTAGTA CGTTGCTTGA CATGTCTGCA ATGTTCCATC AAAAAGCGGA GCAGTACATG 
    GGCAATGTGG ATTCATGGTG CAAAGCTTGT GGGGAAGTGG ATCTTCCATC AGAGCTGCAG 
    GATCTAGAAG ATGCCATTCA TCACCATCAG GGAATATATG AGCATATCAC TTTAGCATAT 
    TCAGAGGTTA GCCAGGATGG TAAATCTTTA TTAGACAAAC TCCAGAGACC ATTGACTCCT 
    GGTAGTTCTG ACTCACTGAC AGCTTCTGCC AACTATTCAA AGGCTGTGCA TCACGTCTTA 
    GATGCAATAC ATGAAGTCTT GCACCACCAG CGTCAGTTGG AAAACATCTG GCAACATCGC 
    AAAGTTCGTC TGCATCAACG CCTACAGCTC TGTGTTTTTC AGCAAGATGT TCAGCAGGTC 
    TTGGATTGGA TAGAGAACCA TGGTGAAGCC TTTCTCAGCA AACACACTGG GGTAGGCAAG 
    TCTCTGCACA GGGCCAGAGC CTTGCAAAAG CGACATGAAG ACTTTGAAGA AGTCGCTCAG 
    AATACATACA CCAATGCTGA TAAGCTGCTG GAAGCTGCAG AACAGTTGGC CCAGACAGGA 
    GAGTGCGATC CAGAGGAAAT ATACCAGGCT GCTCATCAGC TTGAGGATCG GATTCAGGAT 
    TTTGTAAGAC GTGTAGAACA AAGAAAAGTT CTCTTGGACA TGTCTGTATC ATTCCACTCG 
    CATGTTAAAG AGCTATGGAC ATGGCTGGAG GACCTACAAA AGGAACTGCT GGATGATGTC 
    TATGCGGAGT CAGTAGAAGC TGTACAGGAC TTGATCAAAC GGTTTGGACA ACAGCAGCAG 
    ACAACCCTCC AGTTTACAGT AAATGTCATC AAAGAAGGGG AGGATCTAAT TCAGCAACTA 
    AGAGATTCTG CTATATCCAG CAACAAAACT CCTCACAACA GCTCAATCAA TCACATTGAG 
    ACTGTCCTTC AACAACTTGA TGAGGCCCAA TCACAGATGG AGGAGCTTTT CCAAGAGAGA 
    AAAATCAAAC TTGAGCTATT CTTGCAGCTT CGCATCTTTG AGAGAGATGC CATTGATATC 
    ATCTCAGACT TGGAATCATG GAATGATGAA CTTTCTCAGC AAATGAATGA TTTTGACACA 
    GAGGACCTCA CACTAGCCGA GCAAAGGCTA CAGCATCACG CAGACAAAGC TCTGACAATG 
    AACAACCTGA CATTTGATGT CATCCACCAA GGACAAGAGT TACTACAATA TGTGAATGAA 
    GTTCAGAACT CAGGGGTTGA GCTACTCTGT GATAGAGATG TAGATATGGC TACTAGAGTT 
    CAGGACCTGT TGGAATTCCT CCATGAGAAA CAACAGGAGT TGGATCTAGC AGCTGAGCAG 
    CACCGAAAAC ATCTAGAACA ATGTGTGCAA CTACGACATT TGCAAGCTGA AGTCAAACAG 
    GTACTCGGCT GGATACGGAA TGGAGAGTCT ATGTTAAATG CCGGGCTTAT CACTGCCAGT 
    TCATTGCAAG AAGCAGAGCA ACTTCAAAGG GAACATGAGC AGTTCCAGCA TGCAATAGAA 
    AAAACCCACC AGAGTGCTCT GCAGGTGCAG CAGAAGGCAG AGGCCATGCT TCAAGCAAAC 
    CATTACGACA TGGATATGAT CCGGGACTGT GCTGAAAAAG TGGCCTCTCA TTGGCAACAG 
    TTGATGCTTA AAATGGAAGA CCGTCTTAAG CTTGTCAATG CCTCTGTGGC CTTTTATAAA 
    ACCTCAGAGC AGGTATGCAG TGTTCTAGAG AGCCTAGAGC AGGAGTACAA GCGGGAGGAA 
    GATTGGTGTG GCGGAGCTGA TAAACTTGGG CCTAACTCAG AGACTGACCA TGTTACTCCT 
    ATGATCAGCA AACACCTTGA ACAAAAGGAA GCCTTTCTTA AGGCCTGCAC TTTGGCTCGC 
    AGAAATGCTG ATGTCTTTCT GAAATACTTG CACAGGAATA GTGTCAATAT GCCTGGAATG 
    GTGACACATG TAAAGGCTCC TGAACAGCAA GTAAAAAATA TTTTAAATGA GCTGTTTCAG 
    AGGGAAAATA GAGTTCTTCA TTATTGGACC TTGCGGAAAA GGAGACTAGA CCATTGCCAG 
    CAGTATGTAG TATTTGAAAG AAGTGCCAAA CAGGCTCTGG AGTGGATCCA AGATAATGGA 
    GAATTTTACA TTTCCACACA CACCTCCACT GGCTCCACAA TTCAGCATAC ACAAGAACTC 
    TTAAAAGAGC ATGAGGAGTT TCAGATCACA GCAAAGCAAA CCAAAGAGAG GGTAAAGCTG 
    TTGATACAGC TGGCTGATGG CTTTTGTGAA AAAGGGCATG CTCATGCTGC AGAAATTAAA 
    AAATGTGTTA CAGCGGTGGA CAAGAGGTAC AGAGACTTCT CGCTGCGTAT GGAAAAGTAC 
    AGAACCAGCT TAGAGAAGGC TTTGGGCATT TCTTCTGATT CCAAGTCGAG TAAAAGCCTT 
    CAGTTGGATA TTATTCCAGC CACTGCCCCT GGTTCCGAAG TCAAGCTTCG TGATGCAACA 
    CATGAACTCA ATGAGGAGAA AAGAAAATCT GCTCGTCGAA AAGAATTCAT CATGGCAGAA 
    CTTATACAAA CTGAAAAAGC ATATGTTCGT GATCTTCGGG AATGCATGGA TACATATCTG 
    TGGGAAATGA CAAGCGGAGT TGAAGAGATC CCACCAGGCA TTGTAAATAA AGAACACATT 
    ATCTTTGGAA ACATGCAGGA AATATATGAA TTTCATAACA ACATATTCTT AAAGGAGCTT 
    GAGAAGTATG AGCAGCTACC TGAAGATGTT GGACATTGTT TTGTCACATG GGCTGACAAA 
    TTTCAGATGT ATGTGTCCTA CTGTAAGAAT AAGCCTGATT CAACACAACT TATCTTGGAA 
    CAAGCAGGAA CATATTTTGA TGAAATTCAG CAGCGCCATG AGCTGGTCAA CTCCATCTCT 
    TCTTATCTTA TCAAACCTGT GCAAAGGATA ACAAAATATC AGCTTCTTTT AAAAGAGCTG 
    CTGACATGCT GCGAAGAAGG TAAAGGAGAG ATTAAAGATG GGCTGGAAGT GATGCTAAGT 
    GTACCAAAAA GAGCCAACGA TGCAATGCAT CTCAGCATGC TTGAAGGGTT TGATGAGAAC 
    ATTGAATCTC AGGGAGAACT TATTCTGCAA GAGTCTTTTC AAGTGTGGGA TCCTAAAACC 
    TTAATAAGAA AGGGGCGTGA GAGACATCTC TTCTTGTTTG AAATGTCTTT GGTCTTCAGC 
    AAAGAAGTGA AAGATTCAAG TGGAAGGAGC AAATATATTT ACAAATCAAA ATTATTTACC 
    TCAGAGCTTG GAGTCACAGA ACATGTAGAA GGTGATCCTT GCAAATTTGC ACTTTGGGTA 
    GGCAGAACGC CAACATCTGA TAACAAAATA GTCCTTAAGG GCTCAAGTAT TGAGAACAAG 
    CAAGATTGGA TCAAGCATAT AAGAGAAGTG ATCCAAGACC GAACCATTCA CCTGAGAGGC 
    GCATTAAAAG AGCCTATTCA TATACCTAAA ACAGCTGCTG CTACAAAACA GAAACCTGCC 
    AAAAGAGATG GAGAAGACCT GGACAGTCAA GGAGATGGCA GCAGCCAACC TGATACAATA 
    TCTATTGCTT CACGAACTTC ACAGAACACA TTAGACAGTG ATAAGCTTTC TGGTGGATGT 
    GAGCTGACAG TAGTCATCCA TGACTTTACT GCCTGTAATA GCAATGAGCT GACAATTAAA 
    CGAGGGCAGA CTGTGGAGGT TCTAGAGCGG CATCACGATC GACCTGACTG GTGTTTAGTT 
    CGGACTACTG ACCGGTCACC TGCAGCAGAG GGCTTGGTGC CTAGTAGTTC ACTGTGTATT 
    GCACATTCTA GAAGTAGCAT GGAAATGGAA GGCATATTTA ACCACAAAGA TTCCCTCTCT 
    GTCTCCAGTA ATGATGCCAG TCCTCCTGCT TCAGTAATTT CCCTTCAGCC TCACATGATT 
    GGTGCTCAAA GCTCTCCTGG CCCAAAGCGA CCTGGCAATA CTTTGAGAAA ATGGCTCACC 
    AGCCCTGTCA GGAGACTTAG CAGTGGCAAA GCTGATGGAC ATGTGAAAAA ATTGGCTCAT 
    AAACACAAAA AAAGCAGGGA GGTTCGTAAA AACACAGACT CAGGTTCCCA AAAGGACTCT 
    GATGACAGTG CAGCAACACC TCAAGATGAA ACTATCGAGG AGAGAGTGCG CAATGAAGGA 
    CTTAGCAGTG GTACGCTTTC TAAATCCTCC TCGTCTGGCA TGCAAAGCTG TGGAGAAGAA 
    GAGGGCGAGG AAGGACCTGA TGCAGTTCCA TTGCCTCCAC CCATGGCTAT TCAGCAGCAC 
    AGCCTTCTTC AGCCTGACTC ACAGGATGAC AAGACATCAT CTCGGTTGCT GGTGCGACCT 
    ACTAGCTCAG AGACACCTAG TGCTGCAGAG CTTGTTAGTG CTATTGAAGA ACTTGTAAAG 
    AGTAAAATGG CTTTGGAAGA CCGGCCAAGT TCACTTCAAG TAGACCAGGG AGACAGTAGT 
    AGCCCATCAT TCAACCCTTC AGATACATCT CTGCTTTCCT CCTCTTCTCC AATTGATGAA 
    ATGGAAGAAA AAAAATCAAG CTCTTTAAAA AGAAGGCACT ATGTATTACA AGAGCTAGTA 
    GAAACTGAAC GCGATTATGT TCGGGACCTT GGCTATGTGG TTGAGGGTTA TATGGCACTT 
    ATGAAGGAGG ATGGAGTTCC AGATGATATG AAAGGAAAAG ACAAGATTGT TTTTGGCAAC 
    TTACACCAGA TCTACGAGTG GCACAGAGAC TTTTTTTTGG GTGAACTGGA AAGGTGCCTG 
    GATGAGCCTG AAAGATTGGC ATCCCTATTT GTCAAGCATG AAAGAAGATT GCATATGTAC 
    ATAGTTTACT GCCAAAATAA ACCAAAGTCT GAACATATTG TTTCAGAATA CATTGATACC 
    TATTTTGAGG ACCTGAAACA GCGTCTGGGC CATCGACTTC AGCTTACAGA TCTGTTAATA 
    AAACCCATAC AAAGAATAAT GAAATATCAG CTGCTATTAA AGGATTTTCT CAAGTACTCT 
    AAGAAGGCTT GCATAGATGT AGCAGAACTT GAGAGAGCTG TGGAAGTCAT GTGTATCGTA 
    CCGAAGCGGT GTAATGACAT GATGAATGTT GGTCGTTTGC AAGGATTTGA TGGCAAAATA 
    GTTGCCCAAG GAAAGCTGCT ACTGCAAGAC ACATTCCTTG TGACTGAGCA GGACGCTGGT 
    CTTCTGCCCC GATGCAAAGA AAGGCGGGTC TTCCTTTTCG AACAAATTGT CATCTTTAGT 
    GAGCCCTTAG ATAAAAAGAA AGGATACTCT ACACCTGGAT TTCTTTTTAA AAATAATATC 
    AAAGTGAGCT GCCTTAGTTT AGAGGAGTGT GTTGATAGTG ATCCATGCAA GTTTGCACTA 
    TTGTCAAGGT CTGCTGACTC TACAGAGATG TTTGTTTTGC ATTCTTCATC GCCTGGAGTG 
    AGACAGTTAT GGATTCATGA AATCAACCAA ATTTTGGAAA ACCAGCGCAA TTTTTTAAAT 
    GCCTTGACAT CACCAATTGA ATACCACAGA AATCACACTG GTGGAAGCAG TAGTGGCAAC 
    GTCACCTTTA CCAGCAGTCC CAGCAATTGT AGTGGGCTGG GTGGCTCCAG CCGAAGCAGG 
    CCCTCTCGGA TTCCCCAGCC TGTTAGACAC CACTCTCCAG TCCTGGTCTC CTCTGCAGCT 
    TCGGCACAGT CAGAGGCAGA CAAGATGTCA GGTATGTCAG CTCGCCGTGC ACCAGTTGTA 
    CCTCTCAACC TCTCCTTAGA CAATAACCCC AGCCAGAAAG GTAGAGTAAC TGCTGCTCCA 
    GAGACAGGCG GGCGAGATAA AGAATCCAAT TCAGTGCCAA AGATGAAAAT GCTTGATGGC 
    CCCAGGAAAT CAGCTAGTGG CATTCCAACC TTAGCTGATG GTGCACAGAA AGAATTGCGT 
    GGGAGCACAG AAGACAGTCG AATTAGAAGC ACTATGGGCA CTCTAACCTT AGGAAAGCCA 
    AGAAATGGTT CTGCCTCACC CCTCAACTCT CCTGTCACAA TTAATTTTCC ATCGTCTTTT 
    GGTAAGGAAT CAGGGCCACC AAGCAGTCCC CTTCAGAAAG GCTCTTTTTG GAGCTCAATT 
    CCAGGATCTC CAGCCAGCCG ACCTGGCTCC TTCACTTTCC CTGGAGACAG TGACTCTCTC 
    CAGCGACAAG TTCATCGGCA CTCCACACAC AGCAAGGATA CAGACCGCAT GAGCACCTGC 
    TCCTCGACTA GTGAGCAGTC CGTTCACTCC ACTCAGAGTA ATGGGGTAAG GACAGAGAGT 
    GAAAGTAGCA GCAGTAGCAA TATCTCCACC ATGCTGGTTA CGCACGATTA TACAGCGGTG 
    AAGGAGGATG AGATCAATGT CTACCAGGGA GAGGTTGTAC AAATTCTGGC AAGTAACCAA 
    CAGAACATGT TTCTGGTGTT CAGAGCTGCC ACTGATGAGT GCCCTGCAGC TGAGGGCTGG 
    ATTCCAAGCT ATGTTCTGGG TCACACAAGT GCAATCATCA CTGATATGAC TGATGCAACT 
    ATGAAGAAGT CCTCTTCTTG GCATACAGCC CTTAGAATAA GAAAGAAATC TGAGAAGAGG 
    GAAAAAGATG GCAGCAGAGA CACAAAACTA GAGAATGGTT ATCGCAAATC AAGAGAAGGA 
    CTAGCTAATA AGGTATCAGT TAAGCTCCTG AATCCAAACT ACATTTATGA TGCTCCTCCA 
    GAGTTTATTG TACCACTGAA CGAGGTTACA TGTGACACAG GAGAGACTGT GATTATTCGA 
    TGCAAAGTTT GTGGTCGCCC CAAGGCATCC ATTACATGGA AAGGTCCCGA CCATAATACT 
    TTGAGCAACG ATGGACACTA CAGTATATCC TACAGTGATC TTGGAGAAGC AACTCTAAAA 
    ATAGTTGGTG TGACCACTGA GGATGATGGT ATATACACCT GCATTGCAAC AAATGACTTG 
    GGTTCAGTAT CCGCTTCCTC CAATCTTAGA GTGTTAGGTT CATGGAGTGA TGGACTGAGA 
    GTAATCTGGA AAGAAAACTT CGACATTGCT TATAGTGAAG TGGCTGAACT TGGCAGGGGC 
    AGGTTTTCTG TCGTGAAAAA ATGTGAACAA AGAGTTACAA GACGCATAGT AGCCACCAAA 
    TTTGTTAACA AGAAGTTGAT GAAAAGAGAC CAGGTTACCC ATGAACTTGG AATTCTGCAG 
    AGCCTTCAGC ACCCACAACT GATCAGCCTT CTTGACACTT TTGAGACACC AGCAAGCTAT 
    GTCTTAGTAC TTGAGATAGC TGATCAGGGA TGTTTGTTAG AATATGTGGT ACAGTGGGGA 
    AACCTTACAG AAGAAAAGAT CACCTCATAT ATGAGAGAGG TGCTGGAGGC CCTGCAGTAT 
    CTTCATAATT GCAGAATAGC ACATCTGGAC CTAAAGCCTG AGAACATTCT TGTGGATCAG 
    AGCCTAACCA AACCAACTAT AAAACTGTCA GACTTTGGCG ATGCTGTTCA GCTTAATACC 
    ACTTACTACA TTCACCAATT GTTGGGCAGT GCAGAGTTTG CCGCTCCAGA GATCATCCTT 
    GGGAATCCTG TCTCCCTTAC CTCTGATATA TGGAGTATTG GAGTTCTTAC TTATGTCCTT 
    CTCAGTGGCG TCTCTCCTTT CCTTGATGAA AGTGTTGAAG AGACATGCCT GAACATTTGC 
    CGACTAGACT TTAGTTTTCC CGATGACTAC TTTAAGGGAG TAAGTCAGAA GGCCAAAGAA 
    TTTGTATGTT TCTTACTGCA GGGTGACCAG ACAAAGCGCC CTTCTGCTGC TGTATGTCTA 
    AAGGAACAGT GGTTGCAGCC AGGCCAGCAC AAACGTAGTG GCACATTAGA CATGTCAAGA 
    CTGACCTCGT TTATTGAACG CCGTAAACAC CAGAATGATG TTCGACCCAT TGGTAGCATT 
    AAAAACTTCC TTCAGAGTCG CCTTTTACCC AGAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759101.1
    CDS170..9328
    Translation

    Target ORF information:

    RefSeq Version XM_031903241.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis trio Rho guanine nucleotide exchange factor (trio), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031903241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGCAGACTC ATCGCTGGAA AGATGACGGA GAAACAACAT TAGGTTTTCG TAGAAATGAT 
    GAATTGAAAG CAATGGAGGT GTTGCCAATT TTAAAAGAAA AAGTGGCATA TCTATCAGGT 
    GGCAGAGATA AACGAGGTGG TCCTGTATTA ACATTTCCAG CACGCAGCAA CCATGATCGA 
    ATACGACAAG AGGATCTCCG AAGACTGATT TCATATCTTG CTTGTATTCC TAGTGAGGAG 
    GTGTGCAAGC GAGGCTTCAC AGTGATTGTA GATATGCGTG GTTCCAAGTG GGATTCTATT 
    AAACCCTTGC TGAAAATTTT ACAAGAATCC TTTCCATGCT GTATTCATGT TGCACTAATT 
    ATCAAACCAG ATAACTTCTG GCAGAAGCAA AGAACTAATT TTGGAAGTTC TAAGTTTGAA 
    TTTGAGACAA GTATGGTGTC TTTAGAAGGC CTTACCAAAG TAGTTGACCC TTCCCAGCTA 
    ACTCCAGAAT TTGAGGGTTG CTTGGAATAC AACCATGAAG AGTGGATAGA GATCAGAGTA 
    GCATTTGAGG ATTTTATTAA TAATGCAACT CATATGCTGT CTCGATTAGA GGAATTACAG 
    GAGATGCTTG CAATTAAAGA GTTGCCTCAG GATTTAGAGG GGGCACGCAA TATGATTGAA 
    GAACATTCTC AAATGAAAAA AAGGGTTATA AAGGCTCCTA TTGAAGATCT TGATTTAGAA 
    GGGCAAAAGC TTTTACAAAC AATACAAAGC AGTGATAGCT TTCCTAAGAA GAACTCTAGT 
    TCTGGAAATG CAGACCTTCA AAATCTTCTT CCCAAAGTTT CCACAATGTT AGACAGGCTT 
    CATTCAACAC GACAACATCT TCATCAGATG TGGCACATGC GAAAAGTAAA ACTGGACCAG 
    TGCTTTCAGC TAAGGTTGTT TGAGCAGGAT GCTGAGAAGA TGTTTGACTG GATTACACAC 
    AACAAAGGTC TGTTTCTGAC AAGTTATACT GACATTGGCA CCCGTAATAA TCATGCTATG 
    GAACTTCAGA CACAGCACAA TCATTTTGCT ATGAACTGCA TGAATGTCTA TGTAAACATT 
    AGCCGAATAA TGTCTGTGGC AAATCGACTA GTAGAATCTG GACACTATGC CTCACAGCAA 
    ATTAAGCAGA TAGCTAACCA GTTGGAGCAA GAGTGGAAGG CTTTTGCCGC AGCCCTTGAT 
    GAACGTAGTA CGTTGCTTGA CATGTCTGCA ATGTTCCATC AAAAAGCGGA GCAGTACATG 
    GGCAATGTGG ATTCATGGTG CAAAGCTTGT GGGGAAGTGG ATCTTCCATC AGAGCTGCAG 
    GATCTAGAAG ATGCCATTCA TCACCATCAG GGAATATATG AGCATATCAC TTTAGCATAT 
    TCAGAGGTTA GCCAGGATGG TAAATCTTTA TTAGACAAAC TCCAGAGACC ATTGACTCCT 
    GGTAGTTCTG ACTCACTGAC AGCTTCTGCC AACTATTCAA AGGCTGTGCA TCACGTCTTA 
    GATGCAATAC ATGAAGTCTT GCACCACCAG CGTCAGTTGG AAAACATCTG GCAACATCGC 
    AAAGTTCGTC TGCATCAACG CCTACAGCTC TGTGTTTTTC AGCAAGATGT TCAGCAGGTC 
    TTGGATTGGA TAGAGAACCA TGGTGAAGCC TTTCTCAGCA AACACACTGG GGTAGGCAAG 
    TCTCTGCACA GGGCCAGAGC CTTGCAAAAG CGACATGAAG ACTTTGAAGA AGTCGCTCAG 
    AATACATACA CCAATGCTGA TAAGCTGCTG GAAGCTGCAG AACAGTTGGC CCAGACAGGA 
    GAGTGCGATC CAGAGGAAAT ATACCAGGCT GCTCATCAGC TTGAGGATCG GATTCAGGAT 
    TTTGTAAGAC GTGTAGAACA AAGAAAAGTT CTCTTGGACA TGTCTGTATC ATTCCACTCG 
    CATGTTAAAG AGCTATGGAC ATGGCTGGAG GACCTACAAA AGGAACTGCT GGATGATGTC 
    TATGCGGAGT CAGTAGAAGC TGTACAGGAC TTGATCAAAC GGTTTGGACA ACAGCAGCAG 
    ACAACCCTCC AGTTTACAGT AAATGTCATC AAAGAAGGGG AGGATCTAAT TCAGCAACTA 
    AGAGATTCTG CTATATCCAG CAACAAAACT CCTCACAACA GCTCAATCAA TCACATTGAG 
    ACTGTCCTTC AACAACTTGA TGAGGCCCAA TCACAGATGG AGGAGCTTTT CCAAGAGAGA 
    AAAATCAAAC TTGAGCTATT CTTGCAGCTT CGCATCTTTG AGAGAGATGC CATTGATATC 
    ATCTCAGACT TGGAATCATG GAATGATGAA CTTTCTCAGC AAATGAATGA TTTTGACACA 
    GAGGACCTCA CACTAGCCGA GCAAAGGCTA CAGCATCACG CAGACAAAGC TCTGACAATG 
    AACAACCTGA CATTTGATGT CATCCACCAA GGACAAGAGT TACTACAATA TGTGAATGAA 
    GTTCAGAACT CAGGGGTTGA GCTACTCTGT GATAGAGATG TAGATATGGC TACTAGAGTT 
    CAGGACCTGT TGGAATTCCT CCATGAGAAA CAACAGGAGT TGGATCTAGC AGCTGAGCAG 
    CACCGAAAAC ATCTAGAACA ATGTGTGCAA CTACGACATT TGCAAGCTGA AGTCAAACAG 
    GTACTCGGCT GGATACGGAA TGGAGAGTCT ATGTTAAATG CCGGGCTTAT CACTGCCAGT 
    TCATTGCAAG AAGCAGAGCA ACTTCAAAGG GAACATGAGC AGTTCCAGCA TGCAATAGAA 
    AAAACCCACC AGAGTGCTCT GCAGGTGCAG CAGAAGGCAG AGGCCATGCT TCAAGCAAAC 
    CATTACGACA TGGATATGAT CCGGGACTGT GCTGAAAAAG TGGCCTCTCA TTGGCAACAG 
    TTGATGCTTA AAATGGAAGA CCGTCTTAAG CTTGTCAATG CCTCTGTGGC CTTTTATAAA 
    ACCTCAGAGC AGGTATGCAG TGTTCTAGAG AGCCTAGAGC AGGAGTACAA GCGGGAGGAA 
    GATTGGTGTG GCGGAGCTGA TAAACTTGGG CCTAACTCAG AGACTGACCA TGTTACTCCT 
    ATGATCAGCA AACACCTTGA ACAAAAGGAA GCCTTTCTTA AGGCCTGCAC TTTGGCTCGC 
    AGAAATGCTG ATGTCTTTCT GAAATACTTG CACAGGAATA GTGTCAATAT GCCTGGAATG 
    GTGACACATG TAAAGGCTCC TGAACAGCAA GTAAAAAATA TTTTAAATGA GCTGTTTCAG 
    AGGGAAAATA GAGTTCTTCA TTATTGGACC TTGCGGAAAA GGAGACTAGA CCATTGCCAG 
    CAGTATGTAG TATTTGAAAG AAGTGCCAAA CAGGCTCTGG AGTGGATCCA AGATAATGGA 
    GAATTTTACA TTTCCACACA CACCTCCACT GGCTCCACAA TTCAGCATAC ACAAGAACTC 
    TTAAAAGAGC ATGAGGAGTT TCAGATCACA GCAAAGCAAA CCAAAGAGAG GGTAAAGCTG 
    TTGATACAGC TGGCTGATGG CTTTTGTGAA AAAGGGCATG CTCATGCTGC AGAAATTAAA 
    AAATGTGTTA CAGCGGTGGA CAAGAGGTAC AGAGACTTCT CGCTGCGTAT GGAAAAGTAC 
    AGAACCAGCT TAGAGAAGGC TTTGGGCATT TCTTCTGATT CCAAGTCGAG TAAAAGCCTT 
    CAGTTGGATA TTATTCCAGC CACTGCCCCT GGTTCCGAAG TCAAGCTTCG TGATGCAACA 
    CATGAACTCA ATGAGGAGAA AAGAAAATCT GCTCGTCGAA AAGAATTCAT CATGGCAGAA 
    CTTATACAAA CTGAAAAAGC ATATGTTCGT GATCTTCGGG AATGCATGGA TACATATCTG 
    TGGGAAATGA CAAGCGGAGT TGAAGAGATC CCACCAGGCA TTGTAAATAA AGAACACATT 
    ATCTTTGGAA ACATGCAGGA AATATATGAA TTTCATAACA ACATATTCTT AAAGGAGCTT 
    GAGAAGTATG AGCAGCTACC TGAAGATGTT GGACATTGTT TTGTCACATG GGCTGACAAA 
    TTTCAGATGT ATGTGTCCTA CTGTAAGAAT AAGCCTGATT CAACACAACT TATCTTGGAA 
    CAAGCAGGAA CATATTTTGA TGAAATTCAG CAGCGCCATG AGCTGGTCAA CTCCATCTCT 
    TCTTATCTTA TCAAACCTGT GCAAAGGATA ACAAAATATC AGCTTCTTTT AAAAGAGCTG 
    CTGACATGCT GCGAAGAAGG TAAAGGAGAG ATTAAAGATG GGCTGGAAGT GATGCTAAGT 
    GTACCAAAAA GAGCCAACGA TGCAATGCAT CTCAGCATGC TTGAAGGGTT TGATGAGAAC 
    ATTGAATCTC AGGGAGAACT TATTCTGCAA GAGTCTTTTC AAGTGTGGGA TCCTAAAACC 
    TTAATAAGAA AGGGGCGTGA GAGACATCTC TTCTTGTTTG AAATGTCTTT GGTCTTCAGC 
    AAAGAAGTGA AAGATTCAAG TGGAAGGAGC AAATATATTT ACAAATCAAA ATTATTTACC 
    TCAGAGCTTG GAGTCACAGA ACATGTAGAA GGTGATCCTT GCAAATTTGC ACTTTGGGTA 
    GGCAGAACGC CAACATCTGA TAACAAAATA GTCCTTAAGG GCTCAAGTAT TGAGAACAAG 
    CAAGATTGGA TCAAGCATAT AAGAGAAGTG ATCCAAGACC GAACCATTCA CCTGAGAGGC 
    GCATTAAAAG AGCCTATTCA TATACCTAAA ACAGCTGCTG CTACAAAACA GAAACCTGCC 
    AAAAGAGATG GAGAAGACCT GGACAGTCAA GGAGATGGCA GCAGCCAACC TGATACAATA 
    TCTATTGCTT CACGAACTTC ACAGAACACA TTAGACAGTG ATAAGCTTTC TGGTGGATGT 
    GAGCTGACAG TAGTCATCCA TGACTTTACT GCCTGTAATA GCAATGAGCT GACAATTAAA 
    CGAGGGCAGA CTGTGGAGGT TCTAGAGCGG CATCACGATC GACCTGACTG GTGTTTAGTT 
    CGGACTACTG ACCGGTCACC TGCAGCAGAG GGCTTGGTGC CTAGTAGTTC ACTGTGTATT 
    GCACATTCTA GAAGTAGCAT GGAAATGGAA GGCATATTTA ACCACAAAGA TTCCCTCTCT 
    GTCTCCAGTA ATGATGCCAG TCCTCCTGCT TCAGTAATTT CCCTTCAGCC TCACATGATT 
    GGTGCTCAAA GCTCTCCTGG CCCAAAGCGA CCTGGCAATA CTTTGAGAAA ATGGCTCACC 
    AGCCCTGTCA GGAGACTTAG CAGTGGCAAA GCTGATGGAC ATGTGAAAAA ATTGGCTCAT 
    AAACACAAAA AAAGCAGGGA GGTTCGTAAA AACACAGACT CAGGTTCCCA AAAGGACTCT 
    GATGACAGTG CAGCAACACC TCAAGATGAA ACTATCGAGG AGAGAGTGCG CAATGAAGGA 
    CTTAGCAGTG GTACGCTTTC TAAATCCTCC TCGTCTGGCA TGCAAAGCTG TGGAGAAGAA 
    GAGGGCGAGG AAGGACCTGA TGCAGTTCCA TTGCCTCCAC CCATGGCTAT TCAGCAGCAC 
    AGCCTTCTTC AGCCTGACTC ACAGGATGAC AAGACATCAT CTCGGTTGCT GGTGCGACCT 
    ACTAGCTCAG AGACACCTAG TGCTGCAGAG CTTGTTAGTG CTATTGAAGA ACTTGTAAAG 
    AGTAAAATGG CTTTGGAAGA CCGGCCAAGT TCACTTCAAG TAGACCAGGG AGACAGTAGT 
    AGCCCATCAT TCAACCCTTC AGATACATCT CTGCTTTCCT CCTCTTCTCC AATTGATGAA 
    ATGGAAGAAA AAAAATCAAG CTCTTTAAAA AGAAGGCACT ATGTATTACA AGAGCTAGTA 
    GAAACTGAAC GCGATTATGT TCGGGACCTT GGCTATGTGG TTGAGGGTTA TATGGCACTT 
    ATGAAGGAGG ATGGAGTTCC AGATGATATG AAAGGAAAAG ACAAGATTGT TTTTGGCAAC 
    TTACACCAGA TCTACGAGTG GCACAGAGAC TTTTTTTTGG GTGAACTGGA AAGGTGCCTG 
    GATGAGCCTG AAAGATTGGC ATCCCTATTT GTCAAGCATG AAAGAAGATT GCATATGTAC 
    ATAGTTTACT GCCAAAATAA ACCAAAGTCT GAACATATTG TTTCAGAATA CATTGATACC 
    TATTTTGAGG ACCTGAAACA GCGTCTGGGC CATCGACTTC AGCTTACAGA TCTGTTAATA 
    AAACCCATAC AAAGAATAAT GAAATATCAG CTGCTATTAA AGGATTTTCT CAAGTACTCT 
    AAGAAGGCTT GCATAGATGT AGCAGAACTT GAGAGAGCTG TGGAAGTCAT GTGTATCGTA 
    CCGAAGCGGT GTAATGACAT GATGAATGTT GGTCGTTTGC AAGGATTTGA TGGCAAAATA 
    GTTGCCCAAG GAAAGCTGCT ACTGCAAGAC ACATTCCTTG TGACTGAGCA GGACGCTGGT 
    CTTCTGCCCC GATGCAAAGA AAGGCGGGTC TTCCTTTTCG AACAAATTGT CATCTTTAGT 
    GAGCCCTTAG ATAAAAAGAA AGGATACTCT ACACCTGGAT TTCTTTTTAA AAATAATATC 
    AAAGTGAGCT GCCTTAGTTT AGAGGAGTGT GTTGATAGTG ATCCATGCAA GTTTGCACTA 
    TTGTCAAGGT CTGCTGACTC TACAGAGATG TTTGTTTTGC ATTCTTCATC GCCTGGAGTG 
    AGACAGTTAT GGATTCATGA AATCAACCAA ATTTTGGAAA ACCAGCGCAA TTTTTTAAAT 
    GCCTTGACAT CACCAATTGA ATACCACAGA AATCACACTG GTGGAAGCAG TAGTGGCAAC 
    GTCACCTTTA CCAGCAGTCC CAGCAATTGT AGTGGGCTGG GTGGCTCCAG CCGAAGCAGG 
    CCCTCTCGGA TTCCCCAGCC TGTTAGACAC CACTCTCCAG TCCTGGTCTC CTCTGCAGCT 
    TCGGCACAGT CAGAGGCAGA CAAGATGTCA GGTATGTCAG CTCGCCGTGC ACCAGTTGTA 
    CCTCTCAACC TCTCCTTAGA CAATAACCCC AGCCAGAAAG GTAGAGTAAC TGCTGCTCCA 
    GAGACAGGCG GGCGAGATAA AGAATCCAAT TCAGTGCCAA AGATGAAAAT GCTTGATGGC 
    CCCAGGAAAT CAGCTAGTGG CATTCCAACC TTAGCTGATG GTGCACAGAA AGAATTGCGT 
    GGGAGCACAG AAGACAGTCG AATTAGAAGC ACTATGGGCA CTCTAACCTT AGGAAAGCCA 
    AGAAATGGTT CTGCCTCACC CCTCAACTCT CCTGTCACAA TTAATTTTCC ATCGTCTTTT 
    GGTAAGGAAT CAGGGCCACC AAGCAGTCCC CTTCAGAAAG GCTCTTTTTG GAGCTCAATT 
    CCAGGATCTC CAGCCAGCCG ACCTGGCTCC TTCACTTTCC CTGGAGACAG TGACTCTCTC 
    CAGCGACAAG TTCATCGGCA CTCCACACAC AGCAAGGATA CAGACCGCAT GAGCACCTGC 
    TCCTCGACTA GTGAGCAGTC CGTTCACTCC ACTCAGAGTA ATGGGGTAAG GACAGAGAGT 
    GAAAGTAGCA GCAGTAGCAA TATCTCCACC ATGCTGGTTA CGCACGATTA TACAGCGGTG 
    AAGGAGGATG AGATCAATGT CTACCAGGGA GAGGTTGTAC AAATTCTGGC AAGTAACCAA 
    CAGAACATGT TTCTGGTGTT CAGAGCTGCC ACTGATGAGT GCCCTGCAGC TGAGGGCTGG 
    ATTCCAAGCT ATGTTCTGGG TCACACAAGT GCAATCATCA CTGATATGAC TGATGCAACT 
    ATGAAGAAGT CCTCTTCTTG GCATACAGCC CTTAGAATAA GAAAGAAATC TGAGAAGAGG 
    GAAAAAGATG GCAGCAGAGA CACAAAACTA GAGAATGGTT ATCGCAAATC AAGAGAAGGA 
    CTAGCTAATA AGGTATCAGT TAAGCTCCTG AATCCAAACT ACATTTATGA TGCTCCTCCA 
    GAGTTTATTG TACCACTGAA CGAGGTTACA TGTGACACAG GAGAGACTGT GATTATTCGA 
    TGCAAAGTTT GTGGTCGCCC CAAGGCATCC ATTACATGGA AAGGTCCCGA CCATAATACT 
    TTGAGCAACG ATGGACACTA CAGTATATCC TACAGTGATC TTGGAGAAGC AACTCTAAAA 
    ATAGTTGGTG TGACCACTGA GGATGATGGT ATATACACCT GCATTGCAAC AAATGACTTG 
    GGTTCAGTAT CCGCTTCCTC CAATCTTAGA GTGTTAGGTT CATGGAGTGA TGGACTGAGA 
    GTAATCTGGA AAGAAAACTT CGACATTGCT TATAGTGAAG TGGCTGAACT TGGCAGGGGC 
    AGGTTTTCTG TCGTGAAAAA ATGTGAACAA AGAGTTACAA GACGCATAGT AGCCACCAAA 
    TTTGTTAACA AGAAGTTGAT GAAAAGAGAC CAGGTTACCC ATGAACTTGG AATTCTGCAG 
    AGCCTTCAGC ACCCACAACT GATCAGCCTT CTTGACACTT TTGAGACACC AGCAAGCTAT 
    GTCTTAGTAC TTGAGATAGC TGATCAGGGA TGTTTGTTAG AATATGTGGT ACAGTGGGGA 
    AACCTTACAG AAGAAAAGAT CACCTCATAT ATGAGAGAGG TGCTGGAGGC CCTGCAGTAT 
    CTTCATAATT GCAGAATAGC ACATCTGGAC CTAAAGCCTG AGAACATTCT TGTGGATCAG 
    AGCCTAACCA AACCAACTAT AAAACTGTCA GACTTTGGCG ATGCTGTTCA GCTTAATACC 
    ACTTACTACA TTCACCAATT GTTGGGCAGT GCAGAGTTTG CCGCTCCAGA GATCATCCTT 
    GGGAATCCTG TCTCCCTTAC CTCTGATATA TGGAGTATTG GAGTTCTTAC TTATGTCCTT 
    CTCAGTGGCG TCTCTCCTTT CCTTGATGAA AGTGTTGAAG AGACATGCCT GAACATTTGC 
    CGACTAGACT TTAGTTTTCC CGATGACTAC TTTAAGGGAG TAAGTCAGAA GGCCAAAGAA 
    TTTGTATGTT TCTTACTGCA GGGTGACCAG ACAAAGCGCC CTTCTGCTGC TGTATGTCTA 
    AAGGAACAGT GGTTGCAGCC AGGCCAGCAC AAACGTAGTG GCACATTAGA CATGTCAAGA 
    CTGACCTCGT TTATTGAACG CCGTAAACAC CAGAATGATG TTCGACCCAT TGGTAGCATT 
    AAAAACTTCC TTCAGAGTCG CCTTTTACCC AGAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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