TRIO cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIO
Entrez Gene ID	478610
Full Name	trio Rho guanine nucleotide exchange factor
General protein information	Preferred Names trio Rho guanine nucleotide exchange factor Names triple functional domain protein triple functional domain (PTPRF interacting)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022414357.1	XP_022270065.1	triple functional domain protein isoform X3
XM_022414355.1	XP_022270063.1	triple functional domain protein isoform X1
XM_014110313.1	XP_013965788.1	triple functional domain protein isoform X1
XM_014110314.1	XP_013965789.1	triple functional domain protein isoform X2
XM_022414356.1	XP_022270064.1	triple functional domain protein isoform X2
XM_022414358.1	XP_022270066.1	triple functional domain protein isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIO	NP_009049.2
Macaca mulatta (Rhesus monkey)	TRIO	XP_002804369.1
Canis lupus familiaris (dog)	TRIO	XP_005639720.1
Mus musculus (house mouse)	Trio	NP_001074771.1
Danio rerio (zebrafish)	trioa	NP_001097996.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006107	XP_316167.4
Rattus norvegicus (Norway rat)	Trio	XP_003749274.1
Gallus gallus (chicken)	TRIO	XP_419004.4
Pan troglodytes (chimpanzee)	TRIO	XP_517637.3
Caenorhabditis elegans (roundworm)	unc-73	NP_001021496.1
Xenopus tropicalis (tropical clawed frog)	trio	NP_001123393.1
Drosophila melanogaster (fruit fly)	trio	NP_651960.2

Gene Ontology

The following TRIO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe284506	XM_022414357.1 Latest version!	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe284507	XM_022414358.1 Latest version!	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe284505	XM_022414356.1 Latest version!	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe284504	XM_022414355.1 Latest version!	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe44775	XM_014110313.1 Latest version!	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe44776	XM_014110314.1 Latest version!	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIO ( XM_022414357.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270065.1 Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.

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CloneID	OCe284506
Clone ID Related Accession (Same CDS sequence)	XM_022414357.1
Accession Version	XM_022414357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	triple functional domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGGTAA CTGTGACAAG 
ACTACTTTTC ACGTGTGTCT ACATGGGTCA GAAATGGGCT CCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270065.1
CDS	21..5108
Translation	MKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQSLLPKVSAMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHSHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSISHIETVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPAGIVNRELVIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSTELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKGNCDKTTFHVCLHGSEMGSW

Target ORF information:

RefSeq Version	XM_022414357.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414357.1

ORF Insert Sequence:

ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGGTAA CTGTGACAAG 
ACTACTTTTC ACGTGTGTCT ACATGGGTCA GAAATGGGCT CCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_022414358.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270066.1 Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe284507
Clone ID Related Accession (Same CDS sequence)	XM_022414358.1
Accession Version	XM_022414358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	triple functional domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGGACA CATGGAATCA 
ATTCCAGTGC TGTACATATT CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270066.1
CDS	21..5087
Translation	MKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQSLLPKVSAMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHSHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSISHIETVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPAGIVNRELVIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSTELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKGHMESIPVLYIFS

Target ORF information:

RefSeq Version	XM_022414358.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414358.1

ORF Insert Sequence:

ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGGACA CATGGAATCA 
ATTCCAGTGC TGTACATATT CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_022414356.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270064.1 Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe284505
Clone ID Related Accession (Same CDS sequence)	XM_022414356.1
Accession Version	XM_022414356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	triple functional domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC 
TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG 
GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC 
CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC 
GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG 
AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA 
GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC 
AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC 
AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG 
ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA 
AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC 
AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT 
GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT 
CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA 
CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG 
TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC 
GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA 
GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC 
CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA 
GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270064.1
CDS	21..6959
Translation	MKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQSLLPKVSAMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHSHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSISHIETVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPAGIVNRELVIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSTELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHLMGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSTDAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDSGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGGVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGSGGGGGSGGSGGGAGSGAPGGGGSNPSGGPGSCSGLPSSSRSRPSRIPQPVRHHSPVLVSSAASGQAEADKMSE

Target ORF information:

RefSeq Version	XM_022414356.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414356.1

ORF Insert Sequence:

ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC 
TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG 
GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC 
CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC 
GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG 
AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA 
GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC 
AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC 
AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG 
ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA 
AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC 
AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT 
GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT 
CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA 
CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG 
TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC 
GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA 
GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC 
CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA 
GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_022414355.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270063.1 Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

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CloneID	OCe284504
Clone ID Related Accession (Same CDS sequence)	XM_022414355.1
Accession Version	XM_022414355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	triple functional domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC 
TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG 
GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC 
CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC 
GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG 
AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA 
GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC 
AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC 
AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG 
ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA 
AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC 
AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT 
GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT 
CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA 
CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG 
TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC 
GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA 
GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC 
CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA 
GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGGTACGT CCACTCCCGG CCCTGCACTG 
GCCCCTCCCG GCAGCAGCCC CGCCCCGGAG CCCGGCCCCA GCCAGCCTGG CAGGCGCGCC 
CCCGAGCCCG GAGGCCCCGA GCGAGAAGCC GAGCTCATCC CCAGGATGAA GGTGATTGAG 
AGCCCCCGGA GGTGCTCGGG GGGCGCGGCG GGCGCCGGCC GCGATGCGGA CACCAAGGAG 
GCGCGGGCCC CCCCGGAGGA CAGCCGCTCG CGGAGCAGCC CGGGCTCGCT GCCCCTGGCA 
AAGCCCAGGC CTGGGGCCAC CTCGCCTCTG AACTCTCCCG TGGCCGCCGC ATTACCTCCT 
CCAATCGGCA AGGAGCCCTG TCCCCCCAGC AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG 
AGCTCCATCC CCGCCTCCCC CGCCAGCCGG CCAGGCTCCT TCACCTTCCC GGGGGACAGT 
GACTCCCTCC AGCGGCAGGC CCAGCGCCAC GCGGCCCCCA GCAAGGACAC AGACCGCATG 
AGCACCTGCT CGTCGGCCAG CGAGCAGTCG GTACAGTCCA CCCAGAGCAA CGGGAGTGAA 
AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG 
GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAATCAACAG 
AACATGTTTC TGGTGTTCCG AGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT 
CCAGGCTTCG TCCTGGGGCA TACCAGTGCA GTCATCATGG AGAACCCCGA TGGGACTCTC 
AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT 
AAAGACGGCA AGCGGGAAGG CAAGTTAGAG AATGGTTATC GGAAGTCACG GGAGGGGCTC 
AGCAGCAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TATATGACGT TCCCCCAGAA 
TTCGTCATTC CCTTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TTTTAGATGT 
CGGGTTTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAGCA CAACACCCTG 
AACAACGATG GTCACTACAG CATCTCCTAC AGTGACTTGG GAGAGGCCGC TTTGAAGATC 
GTCGGCGTGA CCACAGAAGA TGATGGCATC TACACATGCA TCGCTGTGAA CGACATGGGT 
TCAGCCTCGT CTTCGGCCAG TCTGAGGGTG CTGGGTCCAG GGAGTGATGG GATCATGGTG 
ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGCGAAGTGG CTGAGCTTGG CAGGGGCAGA 
TTCTCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACTAAATTT 
GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AACTTGGAAT CCTGCAGAAC 
CTCCAGCACC CGCTGCTTAT TGGTCTCCTT GATACCTTCG AGACCCCCAC CAGCTACGTC 
CTGGTGTTAG AAATGGCTGA CCAGGGGCGT CTCCTGGACT GTGTGGTGCG ATGGGGAGAC 
CTCACTGAAG GGAAGATCAG GGCGTATCTG GGGGAGGTTC TGGAAGCCGT CCAATACCTT 
CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAAA ACATTCTGGT GGATCAGAGT 
CTGGCCAAGC CCACGATCAA ACTGGCCGAT TTTGGAGATG CTGTCCAACT CAACACGACC 
TACTACATCC ATCAGTTACT GGGGAACCCC GAGTTTGCAG CCCCCGAAAT CATCCTTGGG 
AACCCCATCT CCCTGACCTC GGATACGTGG AGTGTCGGAG TGCTCGCTTA CGTGCTTCTC 
AGCGGCGTGT CCCCCTTCCT CGACGACAGT GTGGAGGAGA CCTGCCTGAA CATTTGCCGA 
CTAGACTTTA GTTTCCCGGA GGACTGCTTT AAAGGTGTGA GCCAGAAGGC CAAGGAGTTC 
GTGTGTTTCC TTCTCCAGGA GGACCCTGCC AGGCGTCCCT CGGCTGCGCT GGCCCTGCAG 
GAGCAGTGGC TGCAGGCGGG CCACGGCGGT GGCAAAGGCG CGGGCGTCCT CGACACGTCC 
AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAACG ATGTTCGACC TATCCGTAGC 
GTTAAAAACT TCTTACAGAG CAGGCTTTTG CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270063.1
CDS	21..9182
Translation	MKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQSLLPKVSAMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHSHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSISHIETVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPAGIVNRELVIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSTELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHLMGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSTDAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDSGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGGVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGSGGGGGSGGSGGGAGSGAPGGGGSNPSGGPGSCSGLPSSSRSRPSRIPQPVRHHSPVLVSSAASGQAEADKMSGTSTPGPALAPPGSSPAPEPGPSQPGRRAPEPGGPEREAELIPRMKVIESPRRCSGGAAGAGRDADTKEARAPPEDSRSRSSPGSLPLAKPRPGATSPLNSPVAAALPPPIGKEPCPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQAQRHAAPSKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIMENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSSKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVFRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEAALKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGSDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQNLQHPLLIGLLDTFETPTSYVLVLEMADQGRLLDCVVRWGDLTEGKIRAYLGEVLEAVQYLHNCRIAHLDLKPENILVDQSLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPISLTSDTWSVGVLAYVLLSGVSPFLDDSVEETCLNICRLDFSFPEDCFKGVSQKAKEFVCFLLQEDPARRPSAALALQEQWLQAGHGGGKGAGVLDTSRLTSFIERRKHQNDVRPIRSVKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_022414355.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414355.1

ORF Insert Sequence:

ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC 
TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG 
GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC 
CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC 
GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG 
AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA 
GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC 
AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC 
AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG 
ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA 
AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC 
AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT 
GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT 
CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA 
CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG 
TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC 
GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA 
GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC 
CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA 
GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGGTACGT CCACTCCCGG CCCTGCACTG 
GCCCCTCCCG GCAGCAGCCC CGCCCCGGAG CCCGGCCCCA GCCAGCCTGG CAGGCGCGCC 
CCCGAGCCCG GAGGCCCCGA GCGAGAAGCC GAGCTCATCC CCAGGATGAA GGTGATTGAG 
AGCCCCCGGA GGTGCTCGGG GGGCGCGGCG GGCGCCGGCC GCGATGCGGA CACCAAGGAG 
GCGCGGGCCC CCCCGGAGGA CAGCCGCTCG CGGAGCAGCC CGGGCTCGCT GCCCCTGGCA 
AAGCCCAGGC CTGGGGCCAC CTCGCCTCTG AACTCTCCCG TGGCCGCCGC ATTACCTCCT 
CCAATCGGCA AGGAGCCCTG TCCCCCCAGC AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG 
AGCTCCATCC CCGCCTCCCC CGCCAGCCGG CCAGGCTCCT TCACCTTCCC GGGGGACAGT 
GACTCCCTCC AGCGGCAGGC CCAGCGCCAC GCGGCCCCCA GCAAGGACAC AGACCGCATG 
AGCACCTGCT CGTCGGCCAG CGAGCAGTCG GTACAGTCCA CCCAGAGCAA CGGGAGTGAA 
AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG 
GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAATCAACAG 
AACATGTTTC TGGTGTTCCG AGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT 
CCAGGCTTCG TCCTGGGGCA TACCAGTGCA GTCATCATGG AGAACCCCGA TGGGACTCTC 
AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT 
AAAGACGGCA AGCGGGAAGG CAAGTTAGAG AATGGTTATC GGAAGTCACG GGAGGGGCTC 
AGCAGCAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TATATGACGT TCCCCCAGAA 
TTCGTCATTC CCTTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TTTTAGATGT 
CGGGTTTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAGCA CAACACCCTG 
AACAACGATG GTCACTACAG CATCTCCTAC AGTGACTTGG GAGAGGCCGC TTTGAAGATC 
GTCGGCGTGA CCACAGAAGA TGATGGCATC TACACATGCA TCGCTGTGAA CGACATGGGT 
TCAGCCTCGT CTTCGGCCAG TCTGAGGGTG CTGGGTCCAG GGAGTGATGG GATCATGGTG 
ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGCGAAGTGG CTGAGCTTGG CAGGGGCAGA 
TTCTCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACTAAATTT 
GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AACTTGGAAT CCTGCAGAAC 
CTCCAGCACC CGCTGCTTAT TGGTCTCCTT GATACCTTCG AGACCCCCAC CAGCTACGTC 
CTGGTGTTAG AAATGGCTGA CCAGGGGCGT CTCCTGGACT GTGTGGTGCG ATGGGGAGAC 
CTCACTGAAG GGAAGATCAG GGCGTATCTG GGGGAGGTTC TGGAAGCCGT CCAATACCTT 
CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAAA ACATTCTGGT GGATCAGAGT 
CTGGCCAAGC CCACGATCAA ACTGGCCGAT TTTGGAGATG CTGTCCAACT CAACACGACC 
TACTACATCC ATCAGTTACT GGGGAACCCC GAGTTTGCAG CCCCCGAAAT CATCCTTGGG 
AACCCCATCT CCCTGACCTC GGATACGTGG AGTGTCGGAG TGCTCGCTTA CGTGCTTCTC 
AGCGGCGTGT CCCCCTTCCT CGACGACAGT GTGGAGGAGA CCTGCCTGAA CATTTGCCGA 
CTAGACTTTA GTTTCCCGGA GGACTGCTTT AAAGGTGTGA GCCAGAAGGC CAAGGAGTTC 
GTGTGTTTCC TTCTCCAGGA GGACCCTGCC AGGCGTCCCT CGGCTGCGCT GGCCCTGCAG 
GAGCAGTGGC TGCAGGCGGG CCACGGCGGT GGCAAAGGCG CGGGCGTCCT CGACACGTCC 
AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAACG ATGTTCGACC TATCCGTAGC 
GTTAAAAACT TCTTACAGAG CAGGCTTTTG CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_014110313.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965788.1 Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

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CloneID	OCe44775
Clone ID Related Accession (Same CDS sequence)	XM_014110313.1
Accession Version	XM_014110313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	triple functional domain protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAT TCTTCATTTC CCTACTTTTT CTAAACCATT GTTCCCTGAA ACTGCTGCTT 
TTAGGGCAGA GATTTAATCT GTCCTTTATT GCTCTATATT TGCCAACAGG AGCAGTGAGT 
TGTATGTGTG GTGGTGCTAA GACCACACAG CGAAGGCTTT CTTGTGCTGC CAGAGATCGA 
AAGGGAGGGT TTCGAAAAAA CGATGAAATG AAAGCTATGG ATGTTTTGCC AATTTTAAAG 
GAAAAAGTTG CATACCTTTC AGGTGGCAGA GATAAACGTG GAGGTCCCAT TTTAACATTT 
CCTGCTCGCA GCAATCATGA TAGAATACGA CAGGAGGACC TCAGGAGACT CATTTCCTAT 
CTAGCCTGTA TCCCTAGTGA AGAGGTCTGC AAGCGAGGAT TCACGGTGAT CGTGGACATG 
CGTGGGTCCA AGTGGGACTC CATTAAGCCT CTGCTGAAGA TATTGCAAGA GTCCTTTCCC 
TGCTGCATTC ATGTTGCACT GATAATCAAG CCAGACAACT TTTGGCAGAA ACAGAGGACT 
AATTTTGGCA GTTCTAAGTT TGAATTTGAG ACAAATATGG TCTCTTTAGA AGGCCTTACC 
AAAGTAGTTG ATCCTTCTCA GCTAACTCCC GAGTTTGATG GCTGCTTGGA ATACAACCAT 
GAAGAATGGA TTGAAATCAG AGTTGCTTTT GAAGACTACA TTAGCAATGC AACCCACATG 
CTCTCTCGGC TGGAGGAACT TCAGGACATC CTAGCCAAGA AAGAATTGCC TCAGGATTTA 
GAGGGGGCTC GGAATATGAT TGAGGAACAT TCCCAGCTAA AGAAGAAGGT GATTAAGGCC 
CCTATAGAAG ACTTAGATTT AGAGGGACAG AAGCTGCTCC AGAGGATTCA GAGCAGTGAC 
AGCTTTCCCA AAAAGAACTC TGGGTCAGGC AATGCGGACC TGCAGAGCCT CCTACCCAAG 
GTGTCCGCCA TGTTGGACAG GCTGCACTCA ACACGGCAGC ATCTGCATCA GATGTGGCAT 
GTACGAAAAC TGAAGCTGGA CCAGTGCTTT CAGCTAAGGC TGTTTGAACA GGACGCTGAA 
AAGATGTTTG ACTGGATCAC ACATAACAAG GGCCTGTTTC TGAACAGCTA CACTGAAATT 
GGGACCAGCC ATCCTCATGC AATGGAACTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TGTATGAACG TGTATGTTAA TATAAATCGC ATAATGTCAG TGGCAAATCG CTTGGTAGAG 
TCTGGCCACT ACGCCTCTCA GCAGATTAAG CAGATTGCGA ATCAGCTGGA GCAGGAGTGG 
AAGGCATTTG CGGCAGCCCT GGATGAGCGG AGTACCTTGC TGGACATGTC CTCCATTTTC 
CACCAGAAGG CTGAAAAGTA TATGAGCAAC GTGGATTCGT GGTGTAAAGC CTGTGGTGAG 
GTAGACCTTC CCTCGGAGCT GCAGGATCTG GAGGATGCCA TTCACCACCA CCAGGGAATA 
TATGAGCACA TCACCCTTGC TTATTCCGAG GTGAGCCAGG ATGGGAAGTC ACTCCTCGAC 
AAGCTCCAGC GGCCTTTGAC TCCTGGCAGC TCCGACTCCC TCACGGCCTC TGCCAACTAC 
TCCAAGGCTG TGCACCATGT CCTTGATGTC ATCCATGAGG TGCTGCACCA CCAGCGACAG 
CTTGAGAACA TCTGGCAGCA CCGCAAGGTC CGGCTCCACC AGCGGCTGCA GCTGTGTGTG 
TTCCAGCAAG ACGTTCAGCA GGTGCTGGAC TGGATTGAGA ACCATGGAGA AGCATTTCTG 
AGCAAACATA CAGGTGTGGG GAAATCTCTT CACCGGGCCA GAGCTTTGCA GAAACGTCAT 
GAAGATTTTG AAGAAGTAGC ACAGAACACA TATACCAACG CAGATAAATT ACTAGAAGCA 
GCAGAACAGC TGGCTCAGAC CGGGGAATGT GACCCAGAAG AGATTTATCA GGCTGCGCAT 
CAGCTTGAAG ACCGGATACA AGATTTTGTT CGGCGCGTAG AGCAGCGCAA GATCCTGCTG 
GACATGTCAG TGTCGTTTCA TAGCCACGTC AAGGAGCTGT GGACATGGCT GGAGGAGCTG 
CAGAAGGAGC TGCTGGACGA TGTGTACGCC GAGTCGGTGG AGGCCGTGCA GGACCTCATC 
AAGCGCTTTG GCCAACAGCA GCAGACGACC CTGCAAGTGA CGGTCAACGT GATCAAGGAA 
GGGGAGGACC TCATACAGCA GCTGAGGGAC TCAGCCATCT CCAGCAACAA GACCCCCCAC 
AACAGCTCCA TCAGTCACAT CGAGACGGTG CTTCAGCAGC TGGACGAGGC CCAGGCCCAG 
ATGGAGGAGC TCTTCCAGGA GCGCAAAATC AAGCTGGAGC TCTTCCTGCA GCTGCGCATA 
TTCGAGAGGG ATGCCATCGA TATCATCTCA GACCTTGAGT CTTGGAACGA TGAGCTTTCT 
CAGCAAATGA ACGACTTTGA CACAGAAGAT CTCACAATAG CAGAACAGCG CCTCCAGCAC 
CATGCAGACA AAGCTTTAAC CATGAACAAC TTGACCTTTG ATGTCATCCA CCAAGGGCAG 
GACCTCTTGC AGTATGTCAA TGAAGTGCAG GCCTCTGGTG TGGAGCTGCT TTGCGATAGA 
GATGTCGACA TGGCCACTCG GGTCCAGGAC CTCCTGGAGT TCCTTCATGA AAAGCAGCAG 
GAACTGGATT TAGCAGCAGA ACAGCATCGG AAGCACTTGG AGCAGTGTGT GCAGCTTCGC 
CATCTGCAGG CTGAAGTGAA GCAGGTGCTG GGCTGGATCC GCAATGGAGA GTCAATGCTG 
AATGCTGGAC TCATCACGGC CAGCTCATTA CAGGAGGCGG AGCAGCTGCA GAGGGAGCAC 
GAGCAGTTCC AGCACGCCAT CGAGAAAACA CATCAAAGCG CCTTGCAGGT GCAGCAGAAG 
GCAGAAGCCA TGCTGCAGGC CAATCACTAT GACATGGACA TGATCAGGGA CTGCGCCGAG 
AAAGTGGCCT CTCACTGGCA GCAGCTCATG CTCAAGATGG AAGACCGCCT CAAGCTTGTG 
AATGCATCTG TGGCCTTCTA CAAAACCTCA GAGCAGGTCT GCAGTGTCCT CGAGAGCCTG 
GAGCAGGAGT ACAAGAGAGA AGAGGACTGG TGTGGAGGAG CCGACAAACT GGGGCCCAAC 
TCAGAGACGG ACCATGTCAC CCCAATGATC AGCAAGCATC TGGAGCAGAA AGAAGCATTC 
CTGAAGGCTT GCACGCTTGC TAGAAGGAAT GCAGATGTCT TCCTGAAATA CCTGCACAGG 
AATAGTGTGA ACATGCCGGG AATGGTGACA CATATCAAAG CCCCTGAACA ACAAGTGAAA 
AATATCTTGA ATGAACTTTT CCAACGGGAG AACAGGGTAC TGCATTATTG GACCATGAGA 
AAGAGACGAC TAGACCAGTG CCAGCAGTAC GTGGTCTTTG AACGAAGTGC CAAGCAGGCT 
TTGGAATGGA TCCATGACAA TGGCGAGTTC TACCTCTCCA CACACACGTC CACGGGCTCC 
AGTATACAGC ACACCCAGGA GCTACTGAAA GAGCACGAGG AGTTTCAGAT CACGGCAAAG 
CAAACCAAAG AGAGAGTGAA GCTATTGATA CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG 
CATGCCCATG CAGCAGAGAT AAAAAAATGT GTCACTGCAG TGGATAAGAG GTACAGAGAT 
TTCTCTCTGC GGATGGAGAA GTACAGGACC TCTTTGGAGA AAGCCCTGGG GATTTCTTCA 
GATTCCAACA AATCGAGCAA AAGTCTTCAG CTAGACATAA TCCCTGCCAG TGTCCCTGGG 
TCAGAGGTGA AGCTTCGTGA TGCTGCTCAT GAACTTAATG AAGAGAAACG GAAGTCGGCC 
CGCAGAAAAG AGTTCATAAT GGCTGAGCTG ATTCAGACTG AAAAGGCTTA CGTGAGAGAC 
CTCCGGGAGT GTATGGATAC ATACCTGTGG GAAATGACCA GCGGAGTGGA GGAGATCCCG 
GCCGGCATCG TAAACAGAGA GCTTGTTATC TTCGGGAACA TGCAGGAAAT CTATGAGTTT 
CATAATAACA TATTCCTAAA AGAGCTGGAA AAATATGAGC AGTTGCCAGA AGATGTTGGA 
CATTGCTTTG TTACTTGGGC AGACAAGTTT CAGATGTATG TCACATATTG CAAAAATAAG 
CCTGATTCTA CTCAGCTGAT ATTAGAGCAT GCAGGATCCT ACTTTGATGA AATACAACAA 
CGGCATGGAT TAGCCAATTC CATCTCTTCC TACCTTATTA AGCCAGTTCA GCGAATAACG 
AAGTATCAAC TTCTTTTAAA AGAACTACTT ACTTGCTGTG AGGAAGGAAA GGGAGAGATA 
AAAGATGGCC TGGAGGTGAT GCTCAGTGTG CCAAAACGAG CCAATGATGC CATGCACCTC 
AGCATGCTGG AAGGGTTTGA TGAAAATATT GAGTCTCAGG GAGAACTCAT TCTACAAGAA 
TCCTTCCAAG TGTGGGACCC AAAAACCTTA ATTCGAAAGG GTCGAGAACG GCATCTCTTC 
CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA GAAGTGAAAG ATTCCAGTGG CAGAAGCAAG 
TACCTTTATA AAAGCAAATT GTTTACCTCA GAGTTGGGTG TCACAGAACA CGTTGAAGGA 
GATCCTTGCA AATTTGCACT GTGGGTGGGG AGGACACCAA CCTCAGATAA TAAAATCGTC 
CTTAAGGCTT CCAGTATAGA AAACAAGCAG GACTGGATAA AGCACATCCG CGAGGTCATA 
CAGGAAAGGA CCATTCACCT GAAAGGAGCC CTGAAGGAGC CCATTCACAT TCCCAAAACG 
GCGCCGGCCA CCAGACAGAA GGGCAGGAGG GATGGAGAGG ATCTGGATAG CCAGGGCGAT 
GGGAGCAGCC AGCCTGACAC AATCTCTATC GCCTCGAGGA CGTCTCAGAA CACACTGGAT 
AGTGATAAGC TCTCTGGCGG CTGCGAGCTG ACAGTGGTGA TCCACGACTT TACCGCCTGC 
AACAGCACAG AGCTGACCAT CCGCCGTGGC CAGACCGTGG AGGTTTTGGA GCGGCCCCAC 
GACAAGCCTG ACTGGTGTCT GGTGCGGACC ACCGACCGGT CCCCTGCTGC AGAAGGCCTG 
GTTCCCTGTG GCTCTCTCTG TATCGCCCAC TCGAGAAGTA GCATGGAAAT GGAGGGTATC 
TTCAACCACA AAGACTCGCT GTCCGTCTCC AGTAACGATG CCAGCCCGCC GGCGTCCGTG 
GCCTCCCTCC AGCCCCACCT GATGGGGGCC CAGAGCTCGC CAGGCCCCAA GCGCCCAGGG 
AACACTCTGC GCAAGTGGCT GACGAGCCCG GTGCGGCGGC TGAGCAGCGG GAAGGCGGAC 
GGGCACGTGA AGAAGCTGGC GCACAAGCAC AAGAAGAGCA GGGAGGTCCG CAAGAGCACC 
GACGCGGGCT CGCAGAAGGA CTCGGACGAC AGCGCGGCCA CCCCGCAGGA CGAGACTGTC 
GAGGAGAGAG GCCGGAATGA AGGCCTGAGC AGTGGCACAC TCTCGAAGTC GTCATCGTCG 
GGCATGCAAA GCTGTGGAGA GGAAGAAGGG GAGGAGGGGG CCGACGCCGT GCCTCTCCCC 
CCGCCCATGG CCATCCAGCA GCACAGCCTC CTCCAGCCGG ATTCACAGGA CGACAAGGCG 
TCTTCTCGGC TATTAGTTCG CCCCACCAGC TCTGAAACGC CAAGCGCAGC CGAGCTCGTG 
AGTGCAATTG AAGAACTCGT GAAAAGCAAG ATGGCCTTGG AGGACCGTCC CAGCTCACTC 
CTTGTTGACC AGGGAGACAG CAGCAGCCCC TCCTTCAACC CTTCAGACAA CTCCCTCCTG 
TCCTCCTCCT CGCCCATTGA TGAGATGGAA GAAAGGAAAT CCAGCTCTTT AAAGAGACGG 
CACTATGTTT TGCAAGAACT AGTGGAGACA GAACGTGACT ATGTGCGAGA CCTCGGCTAT 
GTAGTTGAGG GCTACATGGC TCTCATGAAA GAAGATGGTG TTCCTGATGA CATGAAAGGA 
AAGGACAAAA TTGTATTTGG CAACATCCAT CAGATTTATG ACTGGCACAG AGACTTTTTT 
TTAGGAGAGT TGGAGAAGTG CCTTGAAGAT CCAGAAAAAC TAGGATCCCT TTTTGTTAAA 
CACGAAAGAA GGTTGCACAT GTACATAGTT TATTGTCAAA ATAAACCAAA GTCTGAGCAC 
ATTGTCTCAG AATACATTGA TACCTTTTTT GAGGACTTAA AGCAGCGTCT GGGCCACAGG 
TTGCAGCTCA CAGATCTGTT GATCAAACCA GTGCAGCGGA TTATGAAGTA CCAGCTGCTC 
CTGAAGGACT TCCTCAAGTA TTCCAAAAAA GCTAGCCTGG ATACATCAGA ATTAGAGAGA 
GCTGTGGAAG TCATGTGCAT AGTCCCCAAG CGGTGCAACG ACATGATGAA CGTGGGGCGG 
CTGCAAGGAT TTGATGGTAA AATTGTTGCC CAGGGCAAGC TACTCTTGCA GGACACATTC 
TTGGTCACAG ACCAGGACTC GGGACTTCTG CCTCGCTGTA AAGAGAGGCG AGTTTTCCTC 
TTTGAGCAAA TCGTCATTTT CAGTGAACCA CTTGATAAAA AAAAGGGCTT CTCGATGCCA 
GGATTCCTGT TTAAGAACAG TATCAAGGTG AGTTGCCTTT GCCTGGAGGA AAATGTGGAA 
AATGATCCAT GTAAATTTGC TTTGACGTCG AGGACGGGTG GTGTGGTGGA GACCTTCATT 
TTGCATTCAT CCAGTCCAAG TGTCCGGCAA ACATGGATCC ATGAAATCAA CCAAATTTTA 
GAAAACCAGC GCAATTTTTT AAATGCCTTG ACATCACCAA TCGAGTATCA GAGGAACCAC 
AGTGGGGGCG GCGGCGGGGG CAGTGGCGGG GGCGGCGGCA GCGGAGGCAG CGGCGGCGGG 
GCTGGCAGCG GGGCCCCCGG CGGCGGAGGC AGCAACCCCA GCGGCGGCCC TGGCAGCTGC 
AGCGGCCTCC CCAGCTCGAG CAGAAGCCGA CCCTCCCGGA TCCCCCAGCC TGTCAGACAC 
CACTCCCCCG TGCTGGTCTC CTCTGCAGCC TCGGGCCAGG CAGAGGCAGA CAAGATGTCA 
GGTACGTCCA CTCCCGGCCC TGCACTGGCC CCTCCCGGCA GCAGCCCCGC CCCGGAGCCC 
GGCCCCAGCC AGCCTGGCAG GCGCGCCCCC GAGCCCGGAG GCCCCGAGCG AGAAGCCGAG 
CTCATCCCCA GGATGAAGGT GATTGAGAGC CCCCGGAGGT GCTCGGGGGG CGCGGCGGGC 
GCCGGCCGCG ATGCGGACAC CAAGGAGGCG CGGGCCCCCC CGGAGGACAG CCGCTCGCGG 
AGCAGCCCGG GCTCGCTGCC CCTGGCAAAG CCCAGGCCTG GGGCCACCTC GCCTCTGAAC 
TCTCCCGTGG CCGCCGCATT ACCTCCTCCA ATCGGCAAGG AGCCCTGTCC CCCCAGCAGC 
CCCCTGCAGA AGGGGGGCTC CTTCTGGAGC TCCATCCCCG CCTCCCCCGC CAGCCGGCCA 
GGCTCCTTCA CCTTCCCGGG GGACAGTGAC TCCCTCCAGC GGCAGGCCCA GCGCCACGCG 
GCCCCCAGCA AGGACACAGA CCGCATGAGC ACCTGCTCGT CGGCCAGCGA GCAGTCGGTA 
CAGTCCACCC AGAGCAACGG GAGTGAAAGT AGCAGCAGTA GCAACATCTC CACCATGTTG 
GTGACACACG ATTACACGGC AGTGAAGGAG GATGAGATAA ACGTCTATCA AGGAGAGGTC 
GTTCAAATTC TGGCCAGCAA TCAACAGAAC ATGTTTCTGG TGTTCCGAGC CGCCACTGAC 
CAGTGCCCCG CAGCCGAGGG CTGGATTCCA GGCTTCGTCC TGGGGCATAC CAGTGCAGTC 
ATCATGGAGA ACCCCGATGG GACTCTCAAG AAGTCAACAT CTTGGCACAC AGCACTCCGT 
TTAAGGAAAA AATCTGAGAA AAAAGATAAA GACGGCAAGC GGGAAGGCAA GTTAGAGAAT 
GGTTATCGGA AGTCACGGGA GGGGCTCAGC AGCAAGGTAT CTGTGAAGCT TCTCAATCCC 
AACTACATAT ATGACGTTCC CCCAGAATTC GTCATTCCCT TGAGTGAGGT CACGTGTGAG 
ACAGGGGAGA CCGTTGTTTT TAGATGTCGG GTTTGTGGCC GCCCCAAGGC CTCAATCACC 
TGGAAGGGCC CTGAGCACAA CACCCTGAAC AACGATGGTC ACTACAGCAT CTCCTACAGT 
GACTTGGGAG AGGCCGCTTT GAAGATCGTC GGCGTGACCA CAGAAGATGA TGGCATCTAC 
ACATGCATCG CTGTGAACGA CATGGGTTCA GCCTCGTCTT CGGCCAGTCT GAGGGTGCTG 
GGTCCAGGGA GTGATGGGAT CATGGTGACC TGGAAAGACA ACTTTGACTC CTTCTACAGC 
GAAGTGGCTG AGCTTGGCAG GGGCAGATTC TCTGTCGTTA AGAAATGTGA TCAGAAAGGA 
ACCAAACGAG CAGTGGCCAC TAAATTTGTG AACAAGAAGT TGATGAAGCG TGACCAGGTC 
ACCCATGAAC TTGGAATCCT GCAGAACCTC CAGCACCCGC TGCTTATTGG TCTCCTTGAT 
ACCTTCGAGA CCCCCACCAG CTACGTCCTG GTGTTAGAAA TGGCTGACCA GGGGCGTCTC 
CTGGACTGTG TGGTGCGATG GGGAGACCTC ACTGAAGGGA AGATCAGGGC GTATCTGGGG 
GAGGTTCTGG AAGCCGTCCA ATACCTTCAC AACTGCAGGA TAGCACACCT GGACCTAAAG 
CCTGAAAACA TTCTGGTGGA TCAGAGTCTG GCCAAGCCCA CGATCAAACT GGCCGATTTT 
GGAGATGCTG TCCAACTCAA CACGACCTAC TACATCCATC AGTTACTGGG GAACCCCGAG 
TTTGCAGCCC CCGAAATCAT CCTTGGGAAC CCCATCTCCC TGACCTCGGA TACGTGGAGT 
GTCGGAGTGC TCGCTTACGT GCTTCTCAGC GGCGTGTCCC CCTTCCTCGA CGACAGTGTG 
GAGGAGACCT GCCTGAACAT TTGCCGACTA GACTTTAGTT TCCCGGAGGA CTGCTTTAAA 
GGTGTGAGCC AGAAGGCCAA GGAGTTCGTG TGTTTCCTTC TCCAGGAGGA CCCTGCCAGG 
CGTCCCTCGG CTGCGCTGGC CCTGCAGGAG CAGTGGCTGC AGGCGGGCCA CGGCGGTGGC 
AAAGGCGCGG GCGTCCTCGA CACGTCCAGA CTGACTTCCT TCATTGAGCG GCGCAAACAC 
CAGAACGATG TTCGACCTAT CCGTAGCGTT AAAAACTTCT TACAGAGCAG GCTTTTGCCT 
AGAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965788.1
CDS	16..9384
Translation	MKEFFISLLFLNHCSLKLLLLGQRFNLSFIALYLPTGAVSCMCGGAKTTQRRLSCAARDRKGGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQSLLPKVSAMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHSHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSISHIETVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPAGIVNRELVIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSTELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHLMGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSTDAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDSGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGGVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGSGGGGGSGGSGGGAGSGAPGGGGSNPSGGPGSCSGLPSSSRSRPSRIPQPVRHHSPVLVSSAASGQAEADKMSGTSTPGPALAPPGSSPAPEPGPSQPGRRAPEPGGPEREAELIPRMKVIESPRRCSGGAAGAGRDADTKEARAPPEDSRSRSSPGSLPLAKPRPGATSPLNSPVAAALPPPIGKEPCPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQAQRHAAPSKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIMENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSSKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVFRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEAALKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGSDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQNLQHPLLIGLLDTFETPTSYVLVLEMADQGRLLDCVVRWGDLTEGKIRAYLGEVLEAVQYLHNCRIAHLDLKPENILVDQSLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPISLTSDTWSVGVLAYVLLSGVSPFLDDSVEETCLNICRLDFSFPEDCFKGVSQKAKEFVCFLLQEDPARRPSAALALQEQWLQAGHGGGKGAGVLDTSRLTSFIERRKHQNDVRPIRSVKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_014110313.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110313.1

ORF Insert Sequence:

ATGAAGGAAT TCTTCATTTC CCTACTTTTT CTAAACCATT GTTCCCTGAA ACTGCTGCTT 
TTAGGGCAGA GATTTAATCT GTCCTTTATT GCTCTATATT TGCCAACAGG AGCAGTGAGT 
TGTATGTGTG GTGGTGCTAA GACCACACAG CGAAGGCTTT CTTGTGCTGC CAGAGATCGA 
AAGGGAGGGT TTCGAAAAAA CGATGAAATG AAAGCTATGG ATGTTTTGCC AATTTTAAAG 
GAAAAAGTTG CATACCTTTC AGGTGGCAGA GATAAACGTG GAGGTCCCAT TTTAACATTT 
CCTGCTCGCA GCAATCATGA TAGAATACGA CAGGAGGACC TCAGGAGACT CATTTCCTAT 
CTAGCCTGTA TCCCTAGTGA AGAGGTCTGC AAGCGAGGAT TCACGGTGAT CGTGGACATG 
CGTGGGTCCA AGTGGGACTC CATTAAGCCT CTGCTGAAGA TATTGCAAGA GTCCTTTCCC 
TGCTGCATTC ATGTTGCACT GATAATCAAG CCAGACAACT TTTGGCAGAA ACAGAGGACT 
AATTTTGGCA GTTCTAAGTT TGAATTTGAG ACAAATATGG TCTCTTTAGA AGGCCTTACC 
AAAGTAGTTG ATCCTTCTCA GCTAACTCCC GAGTTTGATG GCTGCTTGGA ATACAACCAT 
GAAGAATGGA TTGAAATCAG AGTTGCTTTT GAAGACTACA TTAGCAATGC AACCCACATG 
CTCTCTCGGC TGGAGGAACT TCAGGACATC CTAGCCAAGA AAGAATTGCC TCAGGATTTA 
GAGGGGGCTC GGAATATGAT TGAGGAACAT TCCCAGCTAA AGAAGAAGGT GATTAAGGCC 
CCTATAGAAG ACTTAGATTT AGAGGGACAG AAGCTGCTCC AGAGGATTCA GAGCAGTGAC 
AGCTTTCCCA AAAAGAACTC TGGGTCAGGC AATGCGGACC TGCAGAGCCT CCTACCCAAG 
GTGTCCGCCA TGTTGGACAG GCTGCACTCA ACACGGCAGC ATCTGCATCA GATGTGGCAT 
GTACGAAAAC TGAAGCTGGA CCAGTGCTTT CAGCTAAGGC TGTTTGAACA GGACGCTGAA 
AAGATGTTTG ACTGGATCAC ACATAACAAG GGCCTGTTTC TGAACAGCTA CACTGAAATT 
GGGACCAGCC ATCCTCATGC AATGGAACTC CAGACACAGC ACAATCACTT TGCCATGAAC 
TGTATGAACG TGTATGTTAA TATAAATCGC ATAATGTCAG TGGCAAATCG CTTGGTAGAG 
TCTGGCCACT ACGCCTCTCA GCAGATTAAG CAGATTGCGA ATCAGCTGGA GCAGGAGTGG 
AAGGCATTTG CGGCAGCCCT GGATGAGCGG AGTACCTTGC TGGACATGTC CTCCATTTTC 
CACCAGAAGG CTGAAAAGTA TATGAGCAAC GTGGATTCGT GGTGTAAAGC CTGTGGTGAG 
GTAGACCTTC CCTCGGAGCT GCAGGATCTG GAGGATGCCA TTCACCACCA CCAGGGAATA 
TATGAGCACA TCACCCTTGC TTATTCCGAG GTGAGCCAGG ATGGGAAGTC ACTCCTCGAC 
AAGCTCCAGC GGCCTTTGAC TCCTGGCAGC TCCGACTCCC TCACGGCCTC TGCCAACTAC 
TCCAAGGCTG TGCACCATGT CCTTGATGTC ATCCATGAGG TGCTGCACCA CCAGCGACAG 
CTTGAGAACA TCTGGCAGCA CCGCAAGGTC CGGCTCCACC AGCGGCTGCA GCTGTGTGTG 
TTCCAGCAAG ACGTTCAGCA GGTGCTGGAC TGGATTGAGA ACCATGGAGA AGCATTTCTG 
AGCAAACATA CAGGTGTGGG GAAATCTCTT CACCGGGCCA GAGCTTTGCA GAAACGTCAT 
GAAGATTTTG AAGAAGTAGC ACAGAACACA TATACCAACG CAGATAAATT ACTAGAAGCA 
GCAGAACAGC TGGCTCAGAC CGGGGAATGT GACCCAGAAG AGATTTATCA GGCTGCGCAT 
CAGCTTGAAG ACCGGATACA AGATTTTGTT CGGCGCGTAG AGCAGCGCAA GATCCTGCTG 
GACATGTCAG TGTCGTTTCA TAGCCACGTC AAGGAGCTGT GGACATGGCT GGAGGAGCTG 
CAGAAGGAGC TGCTGGACGA TGTGTACGCC GAGTCGGTGG AGGCCGTGCA GGACCTCATC 
AAGCGCTTTG GCCAACAGCA GCAGACGACC CTGCAAGTGA CGGTCAACGT GATCAAGGAA 
GGGGAGGACC TCATACAGCA GCTGAGGGAC TCAGCCATCT CCAGCAACAA GACCCCCCAC 
AACAGCTCCA TCAGTCACAT CGAGACGGTG CTTCAGCAGC TGGACGAGGC CCAGGCCCAG 
ATGGAGGAGC TCTTCCAGGA GCGCAAAATC AAGCTGGAGC TCTTCCTGCA GCTGCGCATA 
TTCGAGAGGG ATGCCATCGA TATCATCTCA GACCTTGAGT CTTGGAACGA TGAGCTTTCT 
CAGCAAATGA ACGACTTTGA CACAGAAGAT CTCACAATAG CAGAACAGCG CCTCCAGCAC 
CATGCAGACA AAGCTTTAAC CATGAACAAC TTGACCTTTG ATGTCATCCA CCAAGGGCAG 
GACCTCTTGC AGTATGTCAA TGAAGTGCAG GCCTCTGGTG TGGAGCTGCT TTGCGATAGA 
GATGTCGACA TGGCCACTCG GGTCCAGGAC CTCCTGGAGT TCCTTCATGA AAAGCAGCAG 
GAACTGGATT TAGCAGCAGA ACAGCATCGG AAGCACTTGG AGCAGTGTGT GCAGCTTCGC 
CATCTGCAGG CTGAAGTGAA GCAGGTGCTG GGCTGGATCC GCAATGGAGA GTCAATGCTG 
AATGCTGGAC TCATCACGGC CAGCTCATTA CAGGAGGCGG AGCAGCTGCA GAGGGAGCAC 
GAGCAGTTCC AGCACGCCAT CGAGAAAACA CATCAAAGCG CCTTGCAGGT GCAGCAGAAG 
GCAGAAGCCA TGCTGCAGGC CAATCACTAT GACATGGACA TGATCAGGGA CTGCGCCGAG 
AAAGTGGCCT CTCACTGGCA GCAGCTCATG CTCAAGATGG AAGACCGCCT CAAGCTTGTG 
AATGCATCTG TGGCCTTCTA CAAAACCTCA GAGCAGGTCT GCAGTGTCCT CGAGAGCCTG 
GAGCAGGAGT ACAAGAGAGA AGAGGACTGG TGTGGAGGAG CCGACAAACT GGGGCCCAAC 
TCAGAGACGG ACCATGTCAC CCCAATGATC AGCAAGCATC TGGAGCAGAA AGAAGCATTC 
CTGAAGGCTT GCACGCTTGC TAGAAGGAAT GCAGATGTCT TCCTGAAATA CCTGCACAGG 
AATAGTGTGA ACATGCCGGG AATGGTGACA CATATCAAAG CCCCTGAACA ACAAGTGAAA 
AATATCTTGA ATGAACTTTT CCAACGGGAG AACAGGGTAC TGCATTATTG GACCATGAGA 
AAGAGACGAC TAGACCAGTG CCAGCAGTAC GTGGTCTTTG AACGAAGTGC CAAGCAGGCT 
TTGGAATGGA TCCATGACAA TGGCGAGTTC TACCTCTCCA CACACACGTC CACGGGCTCC 
AGTATACAGC ACACCCAGGA GCTACTGAAA GAGCACGAGG AGTTTCAGAT CACGGCAAAG 
CAAACCAAAG AGAGAGTGAA GCTATTGATA CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG 
CATGCCCATG CAGCAGAGAT AAAAAAATGT GTCACTGCAG TGGATAAGAG GTACAGAGAT 
TTCTCTCTGC GGATGGAGAA GTACAGGACC TCTTTGGAGA AAGCCCTGGG GATTTCTTCA 
GATTCCAACA AATCGAGCAA AAGTCTTCAG CTAGACATAA TCCCTGCCAG TGTCCCTGGG 
TCAGAGGTGA AGCTTCGTGA TGCTGCTCAT GAACTTAATG AAGAGAAACG GAAGTCGGCC 
CGCAGAAAAG AGTTCATAAT GGCTGAGCTG ATTCAGACTG AAAAGGCTTA CGTGAGAGAC 
CTCCGGGAGT GTATGGATAC ATACCTGTGG GAAATGACCA GCGGAGTGGA GGAGATCCCG 
GCCGGCATCG TAAACAGAGA GCTTGTTATC TTCGGGAACA TGCAGGAAAT CTATGAGTTT 
CATAATAACA TATTCCTAAA AGAGCTGGAA AAATATGAGC AGTTGCCAGA AGATGTTGGA 
CATTGCTTTG TTACTTGGGC AGACAAGTTT CAGATGTATG TCACATATTG CAAAAATAAG 
CCTGATTCTA CTCAGCTGAT ATTAGAGCAT GCAGGATCCT ACTTTGATGA AATACAACAA 
CGGCATGGAT TAGCCAATTC CATCTCTTCC TACCTTATTA AGCCAGTTCA GCGAATAACG 
AAGTATCAAC TTCTTTTAAA AGAACTACTT ACTTGCTGTG AGGAAGGAAA GGGAGAGATA 
AAAGATGGCC TGGAGGTGAT GCTCAGTGTG CCAAAACGAG CCAATGATGC CATGCACCTC 
AGCATGCTGG AAGGGTTTGA TGAAAATATT GAGTCTCAGG GAGAACTCAT TCTACAAGAA 
TCCTTCCAAG TGTGGGACCC AAAAACCTTA ATTCGAAAGG GTCGAGAACG GCATCTCTTC 
CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA GAAGTGAAAG ATTCCAGTGG CAGAAGCAAG 
TACCTTTATA AAAGCAAATT GTTTACCTCA GAGTTGGGTG TCACAGAACA CGTTGAAGGA 
GATCCTTGCA AATTTGCACT GTGGGTGGGG AGGACACCAA CCTCAGATAA TAAAATCGTC 
CTTAAGGCTT CCAGTATAGA AAACAAGCAG GACTGGATAA AGCACATCCG CGAGGTCATA 
CAGGAAAGGA CCATTCACCT GAAAGGAGCC CTGAAGGAGC CCATTCACAT TCCCAAAACG 
GCGCCGGCCA CCAGACAGAA GGGCAGGAGG GATGGAGAGG ATCTGGATAG CCAGGGCGAT 
GGGAGCAGCC AGCCTGACAC AATCTCTATC GCCTCGAGGA CGTCTCAGAA CACACTGGAT 
AGTGATAAGC TCTCTGGCGG CTGCGAGCTG ACAGTGGTGA TCCACGACTT TACCGCCTGC 
AACAGCACAG AGCTGACCAT CCGCCGTGGC CAGACCGTGG AGGTTTTGGA GCGGCCCCAC 
GACAAGCCTG ACTGGTGTCT GGTGCGGACC ACCGACCGGT CCCCTGCTGC AGAAGGCCTG 
GTTCCCTGTG GCTCTCTCTG TATCGCCCAC TCGAGAAGTA GCATGGAAAT GGAGGGTATC 
TTCAACCACA AAGACTCGCT GTCCGTCTCC AGTAACGATG CCAGCCCGCC GGCGTCCGTG 
GCCTCCCTCC AGCCCCACCT GATGGGGGCC CAGAGCTCGC CAGGCCCCAA GCGCCCAGGG 
AACACTCTGC GCAAGTGGCT GACGAGCCCG GTGCGGCGGC TGAGCAGCGG GAAGGCGGAC 
GGGCACGTGA AGAAGCTGGC GCACAAGCAC AAGAAGAGCA GGGAGGTCCG CAAGAGCACC 
GACGCGGGCT CGCAGAAGGA CTCGGACGAC AGCGCGGCCA CCCCGCAGGA CGAGACTGTC 
GAGGAGAGAG GCCGGAATGA AGGCCTGAGC AGTGGCACAC TCTCGAAGTC GTCATCGTCG 
GGCATGCAAA GCTGTGGAGA GGAAGAAGGG GAGGAGGGGG CCGACGCCGT GCCTCTCCCC 
CCGCCCATGG CCATCCAGCA GCACAGCCTC CTCCAGCCGG ATTCACAGGA CGACAAGGCG 
TCTTCTCGGC TATTAGTTCG CCCCACCAGC TCTGAAACGC CAAGCGCAGC CGAGCTCGTG 
AGTGCAATTG AAGAACTCGT GAAAAGCAAG ATGGCCTTGG AGGACCGTCC CAGCTCACTC 
CTTGTTGACC AGGGAGACAG CAGCAGCCCC TCCTTCAACC CTTCAGACAA CTCCCTCCTG 
TCCTCCTCCT CGCCCATTGA TGAGATGGAA GAAAGGAAAT CCAGCTCTTT AAAGAGACGG 
CACTATGTTT TGCAAGAACT AGTGGAGACA GAACGTGACT ATGTGCGAGA CCTCGGCTAT 
GTAGTTGAGG GCTACATGGC TCTCATGAAA GAAGATGGTG TTCCTGATGA CATGAAAGGA 
AAGGACAAAA TTGTATTTGG CAACATCCAT CAGATTTATG ACTGGCACAG AGACTTTTTT 
TTAGGAGAGT TGGAGAAGTG CCTTGAAGAT CCAGAAAAAC TAGGATCCCT TTTTGTTAAA 
CACGAAAGAA GGTTGCACAT GTACATAGTT TATTGTCAAA ATAAACCAAA GTCTGAGCAC 
ATTGTCTCAG AATACATTGA TACCTTTTTT GAGGACTTAA AGCAGCGTCT GGGCCACAGG 
TTGCAGCTCA CAGATCTGTT GATCAAACCA GTGCAGCGGA TTATGAAGTA CCAGCTGCTC 
CTGAAGGACT TCCTCAAGTA TTCCAAAAAA GCTAGCCTGG ATACATCAGA ATTAGAGAGA 
GCTGTGGAAG TCATGTGCAT AGTCCCCAAG CGGTGCAACG ACATGATGAA CGTGGGGCGG 
CTGCAAGGAT TTGATGGTAA AATTGTTGCC CAGGGCAAGC TACTCTTGCA GGACACATTC 
TTGGTCACAG ACCAGGACTC GGGACTTCTG CCTCGCTGTA AAGAGAGGCG AGTTTTCCTC 
TTTGAGCAAA TCGTCATTTT CAGTGAACCA CTTGATAAAA AAAAGGGCTT CTCGATGCCA 
GGATTCCTGT TTAAGAACAG TATCAAGGTG AGTTGCCTTT GCCTGGAGGA AAATGTGGAA 
AATGATCCAT GTAAATTTGC TTTGACGTCG AGGACGGGTG GTGTGGTGGA GACCTTCATT 
TTGCATTCAT CCAGTCCAAG TGTCCGGCAA ACATGGATCC ATGAAATCAA CCAAATTTTA 
GAAAACCAGC GCAATTTTTT AAATGCCTTG ACATCACCAA TCGAGTATCA GAGGAACCAC 
AGTGGGGGCG GCGGCGGGGG CAGTGGCGGG GGCGGCGGCA GCGGAGGCAG CGGCGGCGGG 
GCTGGCAGCG GGGCCCCCGG CGGCGGAGGC AGCAACCCCA GCGGCGGCCC TGGCAGCTGC 
AGCGGCCTCC CCAGCTCGAG CAGAAGCCGA CCCTCCCGGA TCCCCCAGCC TGTCAGACAC 
CACTCCCCCG TGCTGGTCTC CTCTGCAGCC TCGGGCCAGG CAGAGGCAGA CAAGATGTCA 
GGTACGTCCA CTCCCGGCCC TGCACTGGCC CCTCCCGGCA GCAGCCCCGC CCCGGAGCCC 
GGCCCCAGCC AGCCTGGCAG GCGCGCCCCC GAGCCCGGAG GCCCCGAGCG AGAAGCCGAG 
CTCATCCCCA GGATGAAGGT GATTGAGAGC CCCCGGAGGT GCTCGGGGGG CGCGGCGGGC 
GCCGGCCGCG ATGCGGACAC CAAGGAGGCG CGGGCCCCCC CGGAGGACAG CCGCTCGCGG 
AGCAGCCCGG GCTCGCTGCC CCTGGCAAAG CCCAGGCCTG GGGCCACCTC GCCTCTGAAC 
TCTCCCGTGG CCGCCGCATT ACCTCCTCCA ATCGGCAAGG AGCCCTGTCC CCCCAGCAGC 
CCCCTGCAGA AGGGGGGCTC CTTCTGGAGC TCCATCCCCG CCTCCCCCGC CAGCCGGCCA 
GGCTCCTTCA CCTTCCCGGG GGACAGTGAC TCCCTCCAGC GGCAGGCCCA GCGCCACGCG 
GCCCCCAGCA AGGACACAGA CCGCATGAGC ACCTGCTCGT CGGCCAGCGA GCAGTCGGTA 
CAGTCCACCC AGAGCAACGG GAGTGAAAGT AGCAGCAGTA GCAACATCTC CACCATGTTG 
GTGACACACG ATTACACGGC AGTGAAGGAG GATGAGATAA ACGTCTATCA AGGAGAGGTC 
GTTCAAATTC TGGCCAGCAA TCAACAGAAC ATGTTTCTGG TGTTCCGAGC CGCCACTGAC 
CAGTGCCCCG CAGCCGAGGG CTGGATTCCA GGCTTCGTCC TGGGGCATAC CAGTGCAGTC 
ATCATGGAGA ACCCCGATGG GACTCTCAAG AAGTCAACAT CTTGGCACAC AGCACTCCGT 
TTAAGGAAAA AATCTGAGAA AAAAGATAAA GACGGCAAGC GGGAAGGCAA GTTAGAGAAT 
GGTTATCGGA AGTCACGGGA GGGGCTCAGC AGCAAGGTAT CTGTGAAGCT TCTCAATCCC 
AACTACATAT ATGACGTTCC CCCAGAATTC GTCATTCCCT TGAGTGAGGT CACGTGTGAG 
ACAGGGGAGA CCGTTGTTTT TAGATGTCGG GTTTGTGGCC GCCCCAAGGC CTCAATCACC 
TGGAAGGGCC CTGAGCACAA CACCCTGAAC AACGATGGTC ACTACAGCAT CTCCTACAGT 
GACTTGGGAG AGGCCGCTTT GAAGATCGTC GGCGTGACCA CAGAAGATGA TGGCATCTAC 
ACATGCATCG CTGTGAACGA CATGGGTTCA GCCTCGTCTT CGGCCAGTCT GAGGGTGCTG 
GGTCCAGGGA GTGATGGGAT CATGGTGACC TGGAAAGACA ACTTTGACTC CTTCTACAGC 
GAAGTGGCTG AGCTTGGCAG GGGCAGATTC TCTGTCGTTA AGAAATGTGA TCAGAAAGGA 
ACCAAACGAG CAGTGGCCAC TAAATTTGTG AACAAGAAGT TGATGAAGCG TGACCAGGTC 
ACCCATGAAC TTGGAATCCT GCAGAACCTC CAGCACCCGC TGCTTATTGG TCTCCTTGAT 
ACCTTCGAGA CCCCCACCAG CTACGTCCTG GTGTTAGAAA TGGCTGACCA GGGGCGTCTC 
CTGGACTGTG TGGTGCGATG GGGAGACCTC ACTGAAGGGA AGATCAGGGC GTATCTGGGG 
GAGGTTCTGG AAGCCGTCCA ATACCTTCAC AACTGCAGGA TAGCACACCT GGACCTAAAG 
CCTGAAAACA TTCTGGTGGA TCAGAGTCTG GCCAAGCCCA CGATCAAACT GGCCGATTTT 
GGAGATGCTG TCCAACTCAA CACGACCTAC TACATCCATC AGTTACTGGG GAACCCCGAG 
TTTGCAGCCC CCGAAATCAT CCTTGGGAAC CCCATCTCCC TGACCTCGGA TACGTGGAGT 
GTCGGAGTGC TCGCTTACGT GCTTCTCAGC GGCGTGTCCC CCTTCCTCGA CGACAGTGTG 
GAGGAGACCT GCCTGAACAT TTGCCGACTA GACTTTAGTT TCCCGGAGGA CTGCTTTAAA 
GGTGTGAGCC AGAAGGCCAA GGAGTTCGTG TGTTTCCTTC TCCAGGAGGA CCCTGCCAGG 
CGTCCCTCGG CTGCGCTGGC CCTGCAGGAG CAGTGGCTGC AGGCGGGCCA CGGCGGTGGC 
AAAGGCGCGG GCGTCCTCGA CACGTCCAGA CTGACTTCCT TCATTGAGCG GCGCAAACAC 
CAGAACGATG TTCGACCTAT CCGTAGCGTT AAAAACTTCT TACAGAGCAG GCTTTTGCCT 
AGAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_014110314.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965789.1 Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

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CloneID	OCe44776
Clone ID Related Accession (Same CDS sequence)	XM_014110314.1
Accession Version	XM_014110314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	triple functional domain protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTT TGGAAATGGA TGAAGAGCAC AGTTTGTCCT GGTTTCGAAA AAACGATGAA 
ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC 
TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG 
GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC 
CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC 
GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG 
AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA 
GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC 
AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC 
AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG 
ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA 
AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC 
AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT 
GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT 
CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA 
CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG 
TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC 
GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA 
GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC 
CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA 
GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGGTACGT CCACTCCCGG CCCTGCACTG 
GCCCCTCCCG GCAGCAGCCC CGCCCCGGAG CCCGGCCCCA GCCAGCCTGG CAGGCGCGCC 
CCCGAGCCCG GAGGCCCCGA GCGAGAAGCC GAGCTCATCC CCAGGATGAA GGTGATTGAG 
AGCCCCCGGA GGTGCTCGGG GGGCGCGGCG GGCGCCGGCC GCGATGCGGA CACCAAGGAG 
GCGCGGGCCC CCCCGGAGGA CAGCCGCTCG CGGAGCAGCC CGGGCTCGCT GCCCCTGGCA 
AAGCCCAGGC CTGGGGCCAC CTCGCCTCTG AACTCTCCCG TGGCCGCCGC ATTACCTCCT 
CCAATCGGCA AGGAGCCCTG TCCCCCCAGC AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG 
AGCTCCATCC CCGCCTCCCC CGCCAGCCGG CCAGGCTCCT TCACCTTCCC GGGGGACAGT 
GACTCCCTCC AGCGGCAGGC CCAGCGCCAC GCGGCCCCCA GCAAGGACAC AGACCGCATG 
AGCACCTGCT CGTCGGCCAG CGAGCAGTCG GTACAGTCCA CCCAGAGCAA CGGGAGTGAA 
AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG 
GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAATCAACAG 
AACATGTTTC TGGTGTTCCG AGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT 
CCAGGCTTCG TCCTGGGGCA TACCAGTGCA GTCATCATGG AGAACCCCGA TGGGACTCTC 
AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT 
AAAGACGGCA AGCGGGAAGG CAAGTTAGAG AATGGTTATC GGAAGTCACG GGAGGGGCTC 
AGCAGCAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TATATGACGT TCCCCCAGAA 
TTCGTCATTC CCTTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TTTTAGATGT 
CGGGTTTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAGCA CAACACCCTG 
AACAACGATG GTCACTACAG CATCTCCTAC AGTGACTTGG GAGAGGCCGC TTTGAAGATC 
GTCGGCGTGA CCACAGAAGA TGATGGCATC TACACATGCA TCGCTGTGAA CGACATGGGT 
TCAGCCTCGT CTTCGGCCAG TCTGAGGGTG CTGGGTCCAG GGAGTGATGG GATCATGGTG 
ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGCGAAGTGG CTGAGCTTGG CAGGGGCAGA 
TTCTCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACTAAATTT 
GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AACTTGGAAT CCTGCAGAAC 
CTCCAGCACC CGCTGCTTAT TGGTCTCCTT GATACCTTCG AGACCCCCAC CAGCTACGTC 
CTGGTGTTAG AAATGGCTGA CCAGGGGCGT CTCCTGGACT GTGTGGTGCG ATGGGGAGAC 
CTCACTGAAG GGAAGATCAG GGCGTATCTG GGGGAGGTTC TGGAAGCCGT CCAATACCTT 
CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAAA ACATTCTGGT GGATCAGAGT 
CTGGCCAAGC CCACGATCAA ACTGGCCGAT TTTGGAGATG CTGTCCAACT CAACACGACC 
TACTACATCC ATCAGTTACT GGGGAACCCC GAGTTTGCAG CCCCCGAAAT CATCCTTGGG 
AACCCCATCT CCCTGACCTC GGATACGTGG AGTGTCGGAG TGCTCGCTTA CGTGCTTCTC 
AGCGGCGTGT CCCCCTTCCT CGACGACAGT GTGGAGGAGA CCTGCCTGAA CATTTGCCGA 
CTAGACTTTA GTTTCCCGGA GGACTGCTTT AAAGGTGTGA GCCAGAAGGC CAAGGAGTTC 
GTGTGTTTCC TTCTCCAGGA GGACCCTGCC AGGCGTCCCT CGGCTGCGCT GGCCCTGCAG 
GAGCAGTGGC TGCAGGCGGG CCACGGCGGT GGCAAAGGCG CGGGCGTCCT CGACACGTCC 
AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAACG ATGTTCGACC TATCCGTAGC 
GTTAAAAACT TCTTACAGAG CAGGCTTTTG CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965789.1
CDS	16..9237
Translation	MRPLEMDEEHSLSWFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQSLLPKVSAMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHSHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSISHIETVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPAGIVNRELVIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSTELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHLMGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSTDAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDSGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGGVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGSGGGGGSGGSGGGAGSGAPGGGGSNPSGGPGSCSGLPSSSRSRPSRIPQPVRHHSPVLVSSAASGQAEADKMSGTSTPGPALAPPGSSPAPEPGPSQPGRRAPEPGGPEREAELIPRMKVIESPRRCSGGAAGAGRDADTKEARAPPEDSRSRSSPGSLPLAKPRPGATSPLNSPVAAALPPPIGKEPCPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQAQRHAAPSKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIMENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSSKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVFRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEAALKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGSDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQNLQHPLLIGLLDTFETPTSYVLVLEMADQGRLLDCVVRWGDLTEGKIRAYLGEVLEAVQYLHNCRIAHLDLKPENILVDQSLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPISLTSDTWSVGVLAYVLLSGVSPFLDDSVEETCLNICRLDFSFPEDCFKGVSQKAKEFVCFLLQEDPARRPSAALALQEQWLQAGHGGGKGAGVLDTSRLTSFIERRKHQNDVRPIRSVKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_014110314.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110314.1

ORF Insert Sequence:

ATGAGGCCTT TGGAAATGGA TGAAGAGCAC AGTTTGTCCT GGTTTCGAAA AAACGATGAA 
ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA 
CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC 
TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC 
AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA 
CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA 
CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA 
GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC 
TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC 
TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC 
AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA 
CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT 
CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT 
AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC 
AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT 
CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC 
GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC 
AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT 
GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG 
GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG 
GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA 
TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC 
GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG 
ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG 
GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG 
GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC 
TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA 
GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC 
AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG 
CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG 
GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT 
CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG 
CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA 
TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA 
ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC 
TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC 
ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC 
TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC 
TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG 
ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG 
AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG 
ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG 
TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG 
AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT 
CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT 
CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG 
CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG 
TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT 
ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG 
GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG 
CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT 
TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA 
CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT 
GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT 
ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG 
AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT 
ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG 
CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT 
GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG 
ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC 
CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC 
TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG 
GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC 
CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC 
GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG 
AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA 
GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC 
AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC 
AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG 
ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA 
AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC 
AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT 
GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT 
CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA 
CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG 
TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC 
GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA 
GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC 
CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA 
GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGGTACGT CCACTCCCGG CCCTGCACTG 
GCCCCTCCCG GCAGCAGCCC CGCCCCGGAG CCCGGCCCCA GCCAGCCTGG CAGGCGCGCC 
CCCGAGCCCG GAGGCCCCGA GCGAGAAGCC GAGCTCATCC CCAGGATGAA GGTGATTGAG 
AGCCCCCGGA GGTGCTCGGG GGGCGCGGCG GGCGCCGGCC GCGATGCGGA CACCAAGGAG 
GCGCGGGCCC CCCCGGAGGA CAGCCGCTCG CGGAGCAGCC CGGGCTCGCT GCCCCTGGCA 
AAGCCCAGGC CTGGGGCCAC CTCGCCTCTG AACTCTCCCG TGGCCGCCGC ATTACCTCCT 
CCAATCGGCA AGGAGCCCTG TCCCCCCAGC AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG 
AGCTCCATCC CCGCCTCCCC CGCCAGCCGG CCAGGCTCCT TCACCTTCCC GGGGGACAGT 
GACTCCCTCC AGCGGCAGGC CCAGCGCCAC GCGGCCCCCA GCAAGGACAC AGACCGCATG 
AGCACCTGCT CGTCGGCCAG CGAGCAGTCG GTACAGTCCA CCCAGAGCAA CGGGAGTGAA 
AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG 
GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAATCAACAG 
AACATGTTTC TGGTGTTCCG AGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT 
CCAGGCTTCG TCCTGGGGCA TACCAGTGCA GTCATCATGG AGAACCCCGA TGGGACTCTC 
AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT 
AAAGACGGCA AGCGGGAAGG CAAGTTAGAG AATGGTTATC GGAAGTCACG GGAGGGGCTC 
AGCAGCAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TATATGACGT TCCCCCAGAA 
TTCGTCATTC CCTTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TTTTAGATGT 
CGGGTTTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAGCA CAACACCCTG 
AACAACGATG GTCACTACAG CATCTCCTAC AGTGACTTGG GAGAGGCCGC TTTGAAGATC 
GTCGGCGTGA CCACAGAAGA TGATGGCATC TACACATGCA TCGCTGTGAA CGACATGGGT 
TCAGCCTCGT CTTCGGCCAG TCTGAGGGTG CTGGGTCCAG GGAGTGATGG GATCATGGTG 
ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGCGAAGTGG CTGAGCTTGG CAGGGGCAGA 
TTCTCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACTAAATTT 
GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AACTTGGAAT CCTGCAGAAC 
CTCCAGCACC CGCTGCTTAT TGGTCTCCTT GATACCTTCG AGACCCCCAC CAGCTACGTC 
CTGGTGTTAG AAATGGCTGA CCAGGGGCGT CTCCTGGACT GTGTGGTGCG ATGGGGAGAC 
CTCACTGAAG GGAAGATCAG GGCGTATCTG GGGGAGGTTC TGGAAGCCGT CCAATACCTT 
CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAAA ACATTCTGGT GGATCAGAGT 
CTGGCCAAGC CCACGATCAA ACTGGCCGAT TTTGGAGATG CTGTCCAACT CAACACGACC 
TACTACATCC ATCAGTTACT GGGGAACCCC GAGTTTGCAG CCCCCGAAAT CATCCTTGGG 
AACCCCATCT CCCTGACCTC GGATACGTGG AGTGTCGGAG TGCTCGCTTA CGTGCTTCTC 
AGCGGCGTGT CCCCCTTCCT CGACGACAGT GTGGAGGAGA CCTGCCTGAA CATTTGCCGA 
CTAGACTTTA GTTTCCCGGA GGACTGCTTT AAAGGTGTGA GCCAGAAGGC CAAGGAGTTC 
GTGTGTTTCC TTCTCCAGGA GGACCCTGCC AGGCGTCCCT CGGCTGCGCT GGCCCTGCAG 
GAGCAGTGGC TGCAGGCGGG CCACGGCGGT GGCAAAGGCG CGGGCGTCCT CGACACGTCC 
AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAACG ATGTTCGACC TATCCGTAGC 
GTTAAAAACT TCTTACAGAG CAGGCTTTTG CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TRIO cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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