TRIO cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIO
Entrez Gene ID	420919
Full Name	trio Rho guanine nucleotide exchange factor
General protein information	Preferred Names trio Rho guanine nucleotide exchange factor Names triple functional domain protein triple functional domain (PTPRF interacting)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015282226.2	XP_015137712.1	triple functional domain protein isoform X1
XM_015282226.1	XP_015137712.1	triple functional domain protein
XM_025147706.1	XP_025003474.1	triple functional domain protein isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIO	NP_009049.2
Macaca mulatta (Rhesus monkey)	TRIO	XP_002804369.1
Canis lupus familiaris (dog)	TRIO	XP_005639720.1
Mus musculus (house mouse)	Trio	NP_001074771.1
Danio rerio (zebrafish)	trioa	NP_001097996.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006107	XP_316167.4
Rattus norvegicus (Norway rat)	Trio	XP_003749274.1
Gallus gallus (chicken)	TRIO	XP_419004.4
Pan troglodytes (chimpanzee)	TRIO	XP_517637.3
Caenorhabditis elegans (roundworm)	unc-73	NP_001021496.1
Xenopus tropicalis (tropical clawed frog)	trio	NP_001123393.1
Drosophila melanogaster (fruit fly)	trio	NP_651960.2

Gene Ontology

The following TRIO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa146191	XM_025147706.1 Latest version!	Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa146190	XM_015282226.2 Latest version!	Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OGa32204	XM_015282226.1 Latest version!	Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIO ( XM_025147706.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025003474.1 Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.

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CloneID	OGa146191
Clone ID Related Accession (Same CDS sequence)	XM_025147706.1
Accession Version	XM_025147706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	triple functional domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG CCGCCACCAC CGCCTCCACC GCCGCGGCCA GCACGGCCGG CAGCGGCGGG 
GGAGAAGGGG CCGAGGAGGC GGCCAAGGAC TCGGCGGACA TCGCGGCGTT CTTCCGATCT 
GGGTTTCGCA AAAATGATGA AATGAAGGCT ATGGAAGTTT TGCCAATTTT AAAGGAAAAA 
GTTGCATACC TTTCAGGTGG CAGAGATAAG CGTGGAGGCC CCATTCTAAC GTTTCCAGCA 
CGTAGTAATC ATGATAGAAT ACGGCAAGAA GACCTTAGGA GACTAATTTC ATACCTAGCT 
TGTATTCCAA GTGAGGAAGT ATGTAAAAGA GGATTCACTG TCATTGTAGA TATGCGTGGG 
TCAAAATGGG ACTCTATAAA GCCTCTGCTG AAGATTTTAC AGGAATCCTT CCCATGTTGT 
ATTCATGTTG CACTGATAAT CAAGCCAGAC AATTTCTGGC AGAAACAGAG GACTAACTTT 
GGCAGCTCTA AGTTTGAGTT TGAGACAAAT ATGGTGTCTC TGGAAGGCCT TACCAAAGTA 
GTTGATCCTT CTCAGCTCAC CCCAGAATTT GATGGCTGTT TGGAGTACAA CCATGAGGAG 
TGGATAGAAA TCAGAGTTGC CTTTGAGGAC TATATTAGCA ATGCAACCCA TATGCTGTCC 
AGACTGGAGG AACTGCAAGA TATATTGTCA AAAAAGGAAA TGCCTCAGGA CTTGGAAGGT 
GCTCGCAATA TGATAGAGGA ACATTCACAG TTAAAAAAGA AAGTCATTAA GGCCCCTATT 
GAGGACCTTG ATGTGGAAGG ACAAAAGTTG CTCCAGCGAA TACAGAGCAG CGATAGCTTT 
CCCAAAAAGA ACTCTGGGTC AGGGAATGCA GACTTACAGA ACCTTTTACC CAAAGTATCC 
ACCATGCTGG ACAGGCTGCA CTCAACACGG CAGCATCTGC ATCAGATGTG GCATGTGCGG 
AAATTGAAAT TGGACCAATG TTTTCAGCTC AGGCTGTTTG AGCAGGATGC TGAAAAGATG 
TTTGACTGGA TCACACACAA CAAAGGTCTG TTTCTGAACA GCTACACAGA AATAGGAACC 
AGTCATCCCC ATGCTATGGA ACTTCAGACA CAGCACAATC ACTTTGCCAT GAACTGTATG 
AATGTGTACG TAAACATAAA TCGCATCATG TCAGTAGCAA ATCGTCTGGT TGAGTCTGGC 
CATTATGCTT CACAGCAAAT AAAACAGATT GCTAGTCAAT TGGAGCAAGA ATGGAAAGCA 
TTTGCTGCAG CCTTGGATGA ACGTAGCACG TTACTGGATA TGTCCTCCAT CTTCCATCAG 
AAGGCTGAAC AGTACATGAG CAATGTGGAT TCATGGTGTA AAGCCTGTGG TGAGGTGGAG 
CTTCCCTCAG AACTGCAAGA TTTAGAAGAT GCTATCCATC ATCATCAGGG AATATATGAG 
CATATTACCT TGGCTTACTC TGAGGTAAGC CAAGATGGAA AGTCACTGCT TGACAAACTT 
CAGAGACCTT TGACTCCTGG AAGTTCCGAT TCCCTCACTG CTTCTGCCAA CTACTCAAAA 
GCAGTTCATC ACGTTCTGGA TGTCATCCAT GAAGTACTCC ACCACCAGCG CCAACTGGAA 
AATATTTGGC AGCATCGAAA GGTTCGCTTG CACCAGCGGC TTCAGCTGTG TGTTTTTCAA 
CAGGATGTTC AACAGGTATT GGATTGGATT GAAAACCATG GAGAAGCCTT TCTTAGCAAA 
CATACTGGAG TGGGAAAATC CCTGCATCGT GCTAGAGCAT TACAGAAACG CCATGAAGAC 
TTTGAAGAAG TTGCACAGAA CACATACACC AACGCAGATA AACTTCTAGA AGCAGCCGAA 
CAGCTTGCTC AGACCGGAGA GTGTGATCCA GAGGAAATAT ATCAAGCTGC CCATCAGCTC 
GAAGACAGGA TACAGGATTT TGTCAGACGC GTGGAGCAGC GCAAGATCCT GCTGGACATG 
TCCGTTTCCT TTCACACACA CGTCAAGGAG CTGTGGACAT GGCTTGAAGA GTTGCAGAAA 
GAACTGCTTG ATGATGTGTA TGCAGAGTCT GTGGAGGCCG TCCAAGACCT GATTAAACGT 
TTTGGTCAGC AGCAGCAGAC CACTCTGCAG GTTACAGTCA ATGTCATAAA AGAAGGCGAA 
GATCTCATAC AGCAACTAAG GGATTCGGCC ATTTCTAGTA ACAAGACTCC CCATAACAGC 
TCGATCAACC ACATAGAGAC AGTCCTGCAG CAGCTGGATG AGGCCCAATC TCAGATGGAG 
GAGCTGTTTC AGGAACGCAA GATCAAGCTT GAGCTGTTTC TGCAGCTCCG CATATTTGAA 
AGAGATGCAA TAGATATAAT TTCAGACCTC GAGTCTTGGA ATGATGAGCT CTCTCAACAG 
ATGAATGACT TTGACACTGA AGATCTTACA ATTGCAGAAC AAAGGCTCCA GCATCATGCA 
GACAAAGCCC TGACCATGAA TAATTTGACC TTTGATGTCA TCCACCAAGG ACAGGATCTG 
CTGCAATATG TCAACGAAGT GCAGGCATCT GGTGTTGAGC TACTTTGTGA CAGGGATGTA 
GACATGGCAA CTCGTGTTCA GGATCTGCTG GAGTTTCTCC ATGAGAAGCA GCAGGAGCTG 
GATGTAGCAG CCGAGCAGCA CCGCAAGCAC TTGGAGCAGT GCGTGCAGCT GCGCCATCTG 
CAGGCGGAGG TCAAGCAGGT CTTGGGCTGG ATCCGCAATG GGGAGTCAAT GTTAAACGCG 
GGCCTTATCA CTGCTAGCTC TTTGCAAGAG GCAGAACAGT TACAGAGGGA ACATGAGCAG 
TTTCAACATG CAATAGAGAA AACACATCAA AGTGCCCTTC AGGTTCAGCA GAAAGCAGAG 
GCAATGCTAC AGGCTAATCA CTATGACATG GATATGATCA GGGAGTGTGC AGAGAAGGTT 
GCTTCCCATT GGCAACAACT GATGCTCAAG ATGGAGGACC GTCTCAAGCT TGTGAATGCC 
TCTGTTGCCT TCTATAAAAC TTCTGAACAG GTGTGCAGTG TATTGGAAAG CCTGGAGCAA 
GAGTATAAGC GGGAAGAAGA CTGGTGTGGG GGAGCAGACA AGCTGGGTCC CAACTCTGAA 
ACAGATCACG TTACCCCCAT GATAAGCAAA CACTTGGAAC AGAAGGAGGC ATTCCTAAAG 
GCTTGCACGC TGGCCCGGAG GAATGCTGAT GTCTTCCTGA AATACCTGCA CAGGAATAGC 
GTAAACATGC CAGGGATGGT GACGCACATC AAAGCACCTG AACAGCAAGT CAAAAATATC 
TTGAATGAAC TCTTCCAGCG GGAGAACCGT GTGCTGCATT ATTGGACCAT GCGTAAAAGA 
CGTCTTGATC AATGCCAACA GTACGTGGTG TTTGAAAGAA GTGCCAAACA GGCTCTGGAA 
TGGATTCATG ACAATGGGGA GTTTTATTTA TCCACACATA CTTCCACTGG TTCCAGTATC 
CAGCATACTC AAGAGCTGTT AAAAGAACAT GAAGAATTTC AGATCACAGC AAAGCAAACC 
AAAGAGAGGG TGAAGTTGTT GATACAGCTG GCCGATGGCT TTTGTGAAAA AGGGCATGCT 
CATGCAACAG AGATTAAAAA ATGTGTCACG GCAGTGGATA AAAGGTACAG AGACTTTTCC 
CTGCGCATGG AGAAATACAG GACCTCTTTA GAGAAAGCTC TGGGTATTTC CTCTGATTCC 
AATAAATCGA GCAAAAGCTT GCAGCTGGAT ATAATCCCAG CCAGTGTCCC TGGGTCAGAA 
GTGAAACTGC GTGATGCTGC TCATGAGCTT AATGAAGAAA AACGAAAATC TGCCCGCAGG 
AAAGAGTTTA TTATGGCTGA GCTAATTCAG ACAGAAAAGG CTTACGTAAG AGACCTCCGA 
GAATGCATGG ATACATATCT ATGGGAAATG ACAAGTGGAG TTGAGGAGAT CCCACCTGGT 
ATTGTAAACA AAGAGCATAT TATCTTTGGA AACATGCAGG AAATCTATGA GTTCCACAAT 
AATATATTCC TCAAGGAGCT AGAAAAATAT GAACAGTTAC CAGAAGATGT TGGACACTGC 
TTTGTGACAT GGGCAGACAA GTTCCAGATG TATGTTACTT ACTGCAAAAA TAAGCCAGAT 
TCTACCCAGC TAATATTAGA ACATGCAGGA GCATACTTTG ATGAGATACA ACAGCGACAT 
GGACTGGCCA ACTCTATCTC TTCTTACCTT ATCAAACCTG TTCAGAGGAT AACAAAATAT 
CAACTTCTTT TAAAGGAGTT GCTCACATGC TGTGAAGAAG GTAAAGGTGA GATAAAAGAT 
GGCCTGGAGG TGATGCTTAG TGTGCCAAAG CGAGCCAATG ATGCCATGCA CCTCAGTATG 
TTGGAAGGCT TTGATGAAAA TATAGAATCT CAGGGAGAGC TCATCCTTCA AGAATCCTTC 
CAAGTGTGGG ACCCCAAAAC TCTAATTCGA AAGGGAAGAG AGAGGCACCT TTTCCTTTTT 
GAGATGTCCT TGGTTTTCAG TAAAGAAGTA AAGGACTCAA GTGGAAGGAG CAAGTACATT 
TACAAGAGTA AACTGTTTAC TTCTGAATTG GGTGTCACAG AACACGTTGA AGGAGATCCC 
TGCAAGTTTG CTCTGTGGGT TGGAAGAACA CCAACTTCAG ACAACAAAAT TGTTCTCAAG 
GCATCCAGTA TAGAGAATAA ACAGGACTGG ATTAAACACA TCCGGGAAGT GATACAAGAA 
AGGACCATTC ATCTGAAAGG AGCATTGAAA GAGCCAATCC ACATCCCGAA AACAGCACCA 
ACAACAAAAC AGAAAGGAAG AAGAGATGGC GAGGACCTGG ACAGTCAAGG AGATGGTAGC 
AGCCAACCTG ATACTATTTC AATTGCCTCA CGAACTTCAC AGAACACACT TGACAGTGAT 
AAGTTGTCTG GTGGGTGCGA GCTGACGGTG GTAATCCATG ATTTCACCGC CTGCAACAGT 
AATGAGCTGA CAATCCGCCG TGGGCAGACT GTGGAGGTCC TGGAGCGACC CCATGACAAA 
CCCGACTGGT GTCTGGTTCG AACTACAGAT CGCTCCCCAG CTGCAGAAGG CCTGGTCCCC 
TGTGGGTCCC TCTGCATTGC TCACTCTCGC AGTAGTATGG AGATGGAGGG GATCTTTAAC 
CACAAAGACT CCCTTTCAGT CTCCAGCAAT GATGCCAGTC CTCCTGCTTC AGTAACATCT 
CTTCAACCCC ATATGATCGG GGCACAGAGC TCCCCGGGTC CCAAGCGCCC TGGCAACACT 
TTGAGAAAAT GGCTTACCAG TCCTGTGAGG CGTCTCAGCA GCGGGAAAGC AGATGGGCAC 
GTCAAAAAAC TGGCCCACAA GCACAAGAAG AGTAGAGAGG TTCGCAAAAG TGCTGATGCT 
GGTTCTCAGA AGGACTCTGA TGATAGTGCA GCAACCCCAC AAGATGAGAC TATTGAAGAG 
AGGGGTCGGA ACGAGGGGCT GAGCAGTGGC ACTCTCTCGA AGTCATCCTC TTCAGGCATG 
CAGAGCTGTG GAGAGGAAGA AGGTGAAGAG GGAGCAGATG CTGTACCACT TCCTCCTCCC 
ATGGCAATTC AGCAACACAG TCTTCTCCAG CCTGATTCAC AGGATGACAA GACCTCTTCT 
CGATTATTGG TGCGGCCTAC CAGCTCTGAG ACGCCCAGTG CTGCAGAACT AGTCAGTGCA 
ATTGAAGAGC TCGTCAAAAG CAAAATGGCC TTGGAAGACC GTCCAAGTTC CTTGTTAGTA 
GATCAAGGTG ACAGCAGCAG TCCATCCTTC AACCCTTCAG ATAACTCTCT TCTCTCCTCA 
TCATCACCTA TAGATGAGAT GGAAGAAAGG AAGTCCAGCT CCTTAAAAAG ACGACACTAT 
GTCTTGCAGG AATTAGTGGA GACAGAGCGT GATTACGTAC GAGATCTGGG CTATGTAGTT 
GAGGGTTATA TGGCACTTAT GAAGGAAGAT GGTGTTCCTG ATGATATGAA AGGCAAAGAC 
AAGATTGTAT TTGGAAATAT TCACCAGATT TATGACTGGC ACAGAGACTT CTTTTTAGGA 
GAGTTGGAGA AGTGCCTTGA AGATCCAGAA AAGCTGGGAT CCCTGTTTGT TAAGCATGAG 
AGAAGGTTGC ACATGTACAT AGTTTACTGC CAGAACAAAC CAAAGTCTGA GCACATTGTC 
TCAGAATACA TTGACACATT TTTTGAGGAT CTAAAGCAAC GTCTGGGCCA CAGACTTCAG 
CTCACAGACT TGTTAATAAA ACCTGTGCAG AGAATTATGA AATACCAGCT ATTACTAAAG 
GATTTCCTCA AATATTCTAA AAAAGCTAAC CTTGATACAA CAGAACTAGA GAGAGCTGTA 
GAGGTCATGT GTATAGTACC AAAACGGTGT AATGACATGA TGAATGTTGG CCGCCTGCAA 
GGATTTGATG GTAAAATTGT AGCCCAGGGT AAGCTGCTAC TCCAGGACAC ATTCTTGGTT 
ACGGACCAGG ACGCAGGTCT TCTGCCACGC TGCAAGGAGA GACGAGTCTT CCTGTTTGAG 
CAGATTGTTA TTTTCAGTGA GCCGCTAGAT AAAAAGAAAG GTTTCTCCAT GCCCGGATTC 
TTGTTTAAAA ACAGTATCAA GGTGAGTTGC CTTTCCCTGG AGGAAAATGT GGACAATGAT 
CCATGTAAAT TTGCACTAAC ATCAAGAACG GGTGATGTCA CGGAGACCTT TGTTTTGCAT 
TCTGCCAGTC CAGGAGTCCG CCAGCTGTGG ATCCATGAAA TTAACCAAAT TTTGGAAAAC 
CAGCGCAACT TTTTAAATGC CTTGACGTCT CCAATCGAGT ACCAGAGGAA CCACAGCAGT 
GGTGGAGGTG GCAGCAGCAG CAGCAGTGGT AACAGCAGCG GCCCCAGCAG CTGTAGCGGA 
ATCCCCAGCT CCAGCCGTAG CCGGCCATCC CGGATCCCCC AGCCTGTCAG ACACCATTCC 
CCAGTCCTGG TCTCCTCTGC AGCCTCGGCC CAAGCAGAGG CAGACAAGAT GTCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025003474.1
CDS	399..7418
Translation	MSSAATTASTAAASTAGSGGGEGAEEAAKDSADIAAFFRSGFRKNDEMKAMEVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILSKKEMPQDLEGARNMIEEHSQLKKKVIKAPIEDLDVEGQKLLQRIQSSDSFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEQYMSNVDSWCKACGEVELPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDVAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRECAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHATEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKEHIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGAYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYIYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPTTKQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVTSLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETIEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKTSSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKANLDTTELERAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLSLEENVDNDPCKFALTSRTGDVTETFVLHSASPGVRQLWIHEINQILENQRNFLNALTSPIEYQRNHSSGGGGSSSSSGNSSGPSSCSGIPSSSRSRPSRIPQPVRHHSPVLVSSAASAQAEADKMSE

Target ORF information:

RefSeq Version	XM_025147706.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147706.1

ORF Insert Sequence:

ATGAGCAGCG CCGCCACCAC CGCCTCCACC GCCGCGGCCA GCACGGCCGG CAGCGGCGGG 
GGAGAAGGGG CCGAGGAGGC GGCCAAGGAC TCGGCGGACA TCGCGGCGTT CTTCCGATCT 
GGGTTTCGCA AAAATGATGA AATGAAGGCT ATGGAAGTTT TGCCAATTTT AAAGGAAAAA 
GTTGCATACC TTTCAGGTGG CAGAGATAAG CGTGGAGGCC CCATTCTAAC GTTTCCAGCA 
CGTAGTAATC ATGATAGAAT ACGGCAAGAA GACCTTAGGA GACTAATTTC ATACCTAGCT 
TGTATTCCAA GTGAGGAAGT ATGTAAAAGA GGATTCACTG TCATTGTAGA TATGCGTGGG 
TCAAAATGGG ACTCTATAAA GCCTCTGCTG AAGATTTTAC AGGAATCCTT CCCATGTTGT 
ATTCATGTTG CACTGATAAT CAAGCCAGAC AATTTCTGGC AGAAACAGAG GACTAACTTT 
GGCAGCTCTA AGTTTGAGTT TGAGACAAAT ATGGTGTCTC TGGAAGGCCT TACCAAAGTA 
GTTGATCCTT CTCAGCTCAC CCCAGAATTT GATGGCTGTT TGGAGTACAA CCATGAGGAG 
TGGATAGAAA TCAGAGTTGC CTTTGAGGAC TATATTAGCA ATGCAACCCA TATGCTGTCC 
AGACTGGAGG AACTGCAAGA TATATTGTCA AAAAAGGAAA TGCCTCAGGA CTTGGAAGGT 
GCTCGCAATA TGATAGAGGA ACATTCACAG TTAAAAAAGA AAGTCATTAA GGCCCCTATT 
GAGGACCTTG ATGTGGAAGG ACAAAAGTTG CTCCAGCGAA TACAGAGCAG CGATAGCTTT 
CCCAAAAAGA ACTCTGGGTC AGGGAATGCA GACTTACAGA ACCTTTTACC CAAAGTATCC 
ACCATGCTGG ACAGGCTGCA CTCAACACGG CAGCATCTGC ATCAGATGTG GCATGTGCGG 
AAATTGAAAT TGGACCAATG TTTTCAGCTC AGGCTGTTTG AGCAGGATGC TGAAAAGATG 
TTTGACTGGA TCACACACAA CAAAGGTCTG TTTCTGAACA GCTACACAGA AATAGGAACC 
AGTCATCCCC ATGCTATGGA ACTTCAGACA CAGCACAATC ACTTTGCCAT GAACTGTATG 
AATGTGTACG TAAACATAAA TCGCATCATG TCAGTAGCAA ATCGTCTGGT TGAGTCTGGC 
CATTATGCTT CACAGCAAAT AAAACAGATT GCTAGTCAAT TGGAGCAAGA ATGGAAAGCA 
TTTGCTGCAG CCTTGGATGA ACGTAGCACG TTACTGGATA TGTCCTCCAT CTTCCATCAG 
AAGGCTGAAC AGTACATGAG CAATGTGGAT TCATGGTGTA AAGCCTGTGG TGAGGTGGAG 
CTTCCCTCAG AACTGCAAGA TTTAGAAGAT GCTATCCATC ATCATCAGGG AATATATGAG 
CATATTACCT TGGCTTACTC TGAGGTAAGC CAAGATGGAA AGTCACTGCT TGACAAACTT 
CAGAGACCTT TGACTCCTGG AAGTTCCGAT TCCCTCACTG CTTCTGCCAA CTACTCAAAA 
GCAGTTCATC ACGTTCTGGA TGTCATCCAT GAAGTACTCC ACCACCAGCG CCAACTGGAA 
AATATTTGGC AGCATCGAAA GGTTCGCTTG CACCAGCGGC TTCAGCTGTG TGTTTTTCAA 
CAGGATGTTC AACAGGTATT GGATTGGATT GAAAACCATG GAGAAGCCTT TCTTAGCAAA 
CATACTGGAG TGGGAAAATC CCTGCATCGT GCTAGAGCAT TACAGAAACG CCATGAAGAC 
TTTGAAGAAG TTGCACAGAA CACATACACC AACGCAGATA AACTTCTAGA AGCAGCCGAA 
CAGCTTGCTC AGACCGGAGA GTGTGATCCA GAGGAAATAT ATCAAGCTGC CCATCAGCTC 
GAAGACAGGA TACAGGATTT TGTCAGACGC GTGGAGCAGC GCAAGATCCT GCTGGACATG 
TCCGTTTCCT TTCACACACA CGTCAAGGAG CTGTGGACAT GGCTTGAAGA GTTGCAGAAA 
GAACTGCTTG ATGATGTGTA TGCAGAGTCT GTGGAGGCCG TCCAAGACCT GATTAAACGT 
TTTGGTCAGC AGCAGCAGAC CACTCTGCAG GTTACAGTCA ATGTCATAAA AGAAGGCGAA 
GATCTCATAC AGCAACTAAG GGATTCGGCC ATTTCTAGTA ACAAGACTCC CCATAACAGC 
TCGATCAACC ACATAGAGAC AGTCCTGCAG CAGCTGGATG AGGCCCAATC TCAGATGGAG 
GAGCTGTTTC AGGAACGCAA GATCAAGCTT GAGCTGTTTC TGCAGCTCCG CATATTTGAA 
AGAGATGCAA TAGATATAAT TTCAGACCTC GAGTCTTGGA ATGATGAGCT CTCTCAACAG 
ATGAATGACT TTGACACTGA AGATCTTACA ATTGCAGAAC AAAGGCTCCA GCATCATGCA 
GACAAAGCCC TGACCATGAA TAATTTGACC TTTGATGTCA TCCACCAAGG ACAGGATCTG 
CTGCAATATG TCAACGAAGT GCAGGCATCT GGTGTTGAGC TACTTTGTGA CAGGGATGTA 
GACATGGCAA CTCGTGTTCA GGATCTGCTG GAGTTTCTCC ATGAGAAGCA GCAGGAGCTG 
GATGTAGCAG CCGAGCAGCA CCGCAAGCAC TTGGAGCAGT GCGTGCAGCT GCGCCATCTG 
CAGGCGGAGG TCAAGCAGGT CTTGGGCTGG ATCCGCAATG GGGAGTCAAT GTTAAACGCG 
GGCCTTATCA CTGCTAGCTC TTTGCAAGAG GCAGAACAGT TACAGAGGGA ACATGAGCAG 
TTTCAACATG CAATAGAGAA AACACATCAA AGTGCCCTTC AGGTTCAGCA GAAAGCAGAG 
GCAATGCTAC AGGCTAATCA CTATGACATG GATATGATCA GGGAGTGTGC AGAGAAGGTT 
GCTTCCCATT GGCAACAACT GATGCTCAAG ATGGAGGACC GTCTCAAGCT TGTGAATGCC 
TCTGTTGCCT TCTATAAAAC TTCTGAACAG GTGTGCAGTG TATTGGAAAG CCTGGAGCAA 
GAGTATAAGC GGGAAGAAGA CTGGTGTGGG GGAGCAGACA AGCTGGGTCC CAACTCTGAA 
ACAGATCACG TTACCCCCAT GATAAGCAAA CACTTGGAAC AGAAGGAGGC ATTCCTAAAG 
GCTTGCACGC TGGCCCGGAG GAATGCTGAT GTCTTCCTGA AATACCTGCA CAGGAATAGC 
GTAAACATGC CAGGGATGGT GACGCACATC AAAGCACCTG AACAGCAAGT CAAAAATATC 
TTGAATGAAC TCTTCCAGCG GGAGAACCGT GTGCTGCATT ATTGGACCAT GCGTAAAAGA 
CGTCTTGATC AATGCCAACA GTACGTGGTG TTTGAAAGAA GTGCCAAACA GGCTCTGGAA 
TGGATTCATG ACAATGGGGA GTTTTATTTA TCCACACATA CTTCCACTGG TTCCAGTATC 
CAGCATACTC AAGAGCTGTT AAAAGAACAT GAAGAATTTC AGATCACAGC AAAGCAAACC 
AAAGAGAGGG TGAAGTTGTT GATACAGCTG GCCGATGGCT TTTGTGAAAA AGGGCATGCT 
CATGCAACAG AGATTAAAAA ATGTGTCACG GCAGTGGATA AAAGGTACAG AGACTTTTCC 
CTGCGCATGG AGAAATACAG GACCTCTTTA GAGAAAGCTC TGGGTATTTC CTCTGATTCC 
AATAAATCGA GCAAAAGCTT GCAGCTGGAT ATAATCCCAG CCAGTGTCCC TGGGTCAGAA 
GTGAAACTGC GTGATGCTGC TCATGAGCTT AATGAAGAAA AACGAAAATC TGCCCGCAGG 
AAAGAGTTTA TTATGGCTGA GCTAATTCAG ACAGAAAAGG CTTACGTAAG AGACCTCCGA 
GAATGCATGG ATACATATCT ATGGGAAATG ACAAGTGGAG TTGAGGAGAT CCCACCTGGT 
ATTGTAAACA AAGAGCATAT TATCTTTGGA AACATGCAGG AAATCTATGA GTTCCACAAT 
AATATATTCC TCAAGGAGCT AGAAAAATAT GAACAGTTAC CAGAAGATGT TGGACACTGC 
TTTGTGACAT GGGCAGACAA GTTCCAGATG TATGTTACTT ACTGCAAAAA TAAGCCAGAT 
TCTACCCAGC TAATATTAGA ACATGCAGGA GCATACTTTG ATGAGATACA ACAGCGACAT 
GGACTGGCCA ACTCTATCTC TTCTTACCTT ATCAAACCTG TTCAGAGGAT AACAAAATAT 
CAACTTCTTT TAAAGGAGTT GCTCACATGC TGTGAAGAAG GTAAAGGTGA GATAAAAGAT 
GGCCTGGAGG TGATGCTTAG TGTGCCAAAG CGAGCCAATG ATGCCATGCA CCTCAGTATG 
TTGGAAGGCT TTGATGAAAA TATAGAATCT CAGGGAGAGC TCATCCTTCA AGAATCCTTC 
CAAGTGTGGG ACCCCAAAAC TCTAATTCGA AAGGGAAGAG AGAGGCACCT TTTCCTTTTT 
GAGATGTCCT TGGTTTTCAG TAAAGAAGTA AAGGACTCAA GTGGAAGGAG CAAGTACATT 
TACAAGAGTA AACTGTTTAC TTCTGAATTG GGTGTCACAG AACACGTTGA AGGAGATCCC 
TGCAAGTTTG CTCTGTGGGT TGGAAGAACA CCAACTTCAG ACAACAAAAT TGTTCTCAAG 
GCATCCAGTA TAGAGAATAA ACAGGACTGG ATTAAACACA TCCGGGAAGT GATACAAGAA 
AGGACCATTC ATCTGAAAGG AGCATTGAAA GAGCCAATCC ACATCCCGAA AACAGCACCA 
ACAACAAAAC AGAAAGGAAG AAGAGATGGC GAGGACCTGG ACAGTCAAGG AGATGGTAGC 
AGCCAACCTG ATACTATTTC AATTGCCTCA CGAACTTCAC AGAACACACT TGACAGTGAT 
AAGTTGTCTG GTGGGTGCGA GCTGACGGTG GTAATCCATG ATTTCACCGC CTGCAACAGT 
AATGAGCTGA CAATCCGCCG TGGGCAGACT GTGGAGGTCC TGGAGCGACC CCATGACAAA 
CCCGACTGGT GTCTGGTTCG AACTACAGAT CGCTCCCCAG CTGCAGAAGG CCTGGTCCCC 
TGTGGGTCCC TCTGCATTGC TCACTCTCGC AGTAGTATGG AGATGGAGGG GATCTTTAAC 
CACAAAGACT CCCTTTCAGT CTCCAGCAAT GATGCCAGTC CTCCTGCTTC AGTAACATCT 
CTTCAACCCC ATATGATCGG GGCACAGAGC TCCCCGGGTC CCAAGCGCCC TGGCAACACT 
TTGAGAAAAT GGCTTACCAG TCCTGTGAGG CGTCTCAGCA GCGGGAAAGC AGATGGGCAC 
GTCAAAAAAC TGGCCCACAA GCACAAGAAG AGTAGAGAGG TTCGCAAAAG TGCTGATGCT 
GGTTCTCAGA AGGACTCTGA TGATAGTGCA GCAACCCCAC AAGATGAGAC TATTGAAGAG 
AGGGGTCGGA ACGAGGGGCT GAGCAGTGGC ACTCTCTCGA AGTCATCCTC TTCAGGCATG 
CAGAGCTGTG GAGAGGAAGA AGGTGAAGAG GGAGCAGATG CTGTACCACT TCCTCCTCCC 
ATGGCAATTC AGCAACACAG TCTTCTCCAG CCTGATTCAC AGGATGACAA GACCTCTTCT 
CGATTATTGG TGCGGCCTAC CAGCTCTGAG ACGCCCAGTG CTGCAGAACT AGTCAGTGCA 
ATTGAAGAGC TCGTCAAAAG CAAAATGGCC TTGGAAGACC GTCCAAGTTC CTTGTTAGTA 
GATCAAGGTG ACAGCAGCAG TCCATCCTTC AACCCTTCAG ATAACTCTCT TCTCTCCTCA 
TCATCACCTA TAGATGAGAT GGAAGAAAGG AAGTCCAGCT CCTTAAAAAG ACGACACTAT 
GTCTTGCAGG AATTAGTGGA GACAGAGCGT GATTACGTAC GAGATCTGGG CTATGTAGTT 
GAGGGTTATA TGGCACTTAT GAAGGAAGAT GGTGTTCCTG ATGATATGAA AGGCAAAGAC 
AAGATTGTAT TTGGAAATAT TCACCAGATT TATGACTGGC ACAGAGACTT CTTTTTAGGA 
GAGTTGGAGA AGTGCCTTGA AGATCCAGAA AAGCTGGGAT CCCTGTTTGT TAAGCATGAG 
AGAAGGTTGC ACATGTACAT AGTTTACTGC CAGAACAAAC CAAAGTCTGA GCACATTGTC 
TCAGAATACA TTGACACATT TTTTGAGGAT CTAAAGCAAC GTCTGGGCCA CAGACTTCAG 
CTCACAGACT TGTTAATAAA ACCTGTGCAG AGAATTATGA AATACCAGCT ATTACTAAAG 
GATTTCCTCA AATATTCTAA AAAAGCTAAC CTTGATACAA CAGAACTAGA GAGAGCTGTA 
GAGGTCATGT GTATAGTACC AAAACGGTGT AATGACATGA TGAATGTTGG CCGCCTGCAA 
GGATTTGATG GTAAAATTGT AGCCCAGGGT AAGCTGCTAC TCCAGGACAC ATTCTTGGTT 
ACGGACCAGG ACGCAGGTCT TCTGCCACGC TGCAAGGAGA GACGAGTCTT CCTGTTTGAG 
CAGATTGTTA TTTTCAGTGA GCCGCTAGAT AAAAAGAAAG GTTTCTCCAT GCCCGGATTC 
TTGTTTAAAA ACAGTATCAA GGTGAGTTGC CTTTCCCTGG AGGAAAATGT GGACAATGAT 
CCATGTAAAT TTGCACTAAC ATCAAGAACG GGTGATGTCA CGGAGACCTT TGTTTTGCAT 
TCTGCCAGTC CAGGAGTCCG CCAGCTGTGG ATCCATGAAA TTAACCAAAT TTTGGAAAAC 
CAGCGCAACT TTTTAAATGC CTTGACGTCT CCAATCGAGT ACCAGAGGAA CCACAGCAGT 
GGTGGAGGTG GCAGCAGCAG CAGCAGTGGT AACAGCAGCG GCCCCAGCAG CTGTAGCGGA 
ATCCCCAGCT CCAGCCGTAG CCGGCCATCC CGGATCCCCC AGCCTGTCAG ACACCATTCC 
CCAGTCCTGG TCTCCTCTGC AGCCTCGGCC CAAGCAGAGG CAGACAAGAT GTCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_015282226.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137712.1 Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

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CloneID	OGa146190
Clone ID Related Accession (Same CDS sequence)	XM_015282226.2
Accession Version	XM_015282226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	triple functional domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282226.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG CCGCCACCAC CGCCTCCACC GCCGCGGCCA GCACGGCCGG CAGCGGCGGG 
GGAGAAGGGG CCGAGGAGGC GGCCAAGGAC TCGGCGGACA TCGCGGCGTT CTTCCGATCT 
GGGTTTCGCA AAAATGATGA AATGAAGGCT ATGGAAGTTT TGCCAATTTT AAAGGAAAAA 
GTTGCATACC TTTCAGGTGG CAGAGATAAG CGTGGAGGCC CCATTCTAAC GTTTCCAGCA 
CGTAGTAATC ATGATAGAAT ACGGCAAGAA GACCTTAGGA GACTAATTTC ATACCTAGCT 
TGTATTCCAA GTGAGGAAGT ATGTAAAAGA GGATTCACTG TCATTGTAGA TATGCGTGGG 
TCAAAATGGG ACTCTATAAA GCCTCTGCTG AAGATTTTAC AGGAATCCTT CCCATGTTGT 
ATTCATGTTG CACTGATAAT CAAGCCAGAC AATTTCTGGC AGAAACAGAG GACTAACTTT 
GGCAGCTCTA AGTTTGAGTT TGAGACAAAT ATGGTGTCTC TGGAAGGCCT TACCAAAGTA 
GTTGATCCTT CTCAGCTCAC CCCAGAATTT GATGGCTGTT TGGAGTACAA CCATGAGGAG 
TGGATAGAAA TCAGAGTTGC CTTTGAGGAC TATATTAGCA ATGCAACCCA TATGCTGTCC 
AGACTGGAGG AACTGCAAGA TATATTGTCA AAAAAGGAAA TGCCTCAGGA CTTGGAAGGT 
GCTCGCAATA TGATAGAGGA ACATTCACAG TTAAAAAAGA AAGTCATTAA GGCCCCTATT 
GAGGACCTTG ATGTGGAAGG ACAAAAGTTG CTCCAGCGAA TACAGAGCAG CGATAGCTTT 
CCCAAAAAGA ACTCTGGGTC AGGGAATGCA GACTTACAGA ACCTTTTACC CAAAGTATCC 
ACCATGCTGG ACAGGCTGCA CTCAACACGG CAGCATCTGC ATCAGATGTG GCATGTGCGG 
AAATTGAAAT TGGACCAATG TTTTCAGCTC AGGCTGTTTG AGCAGGATGC TGAAAAGATG 
TTTGACTGGA TCACACACAA CAAAGGTCTG TTTCTGAACA GCTACACAGA AATAGGAACC 
AGTCATCCCC ATGCTATGGA ACTTCAGACA CAGCACAATC ACTTTGCCAT GAACTGTATG 
AATGTGTACG TAAACATAAA TCGCATCATG TCAGTAGCAA ATCGTCTGGT TGAGTCTGGC 
CATTATGCTT CACAGCAAAT AAAACAGATT GCTAGTCAAT TGGAGCAAGA ATGGAAAGCA 
TTTGCTGCAG CCTTGGATGA ACGTAGCACG TTACTGGATA TGTCCTCCAT CTTCCATCAG 
AAGGCTGAAC AGTACATGAG CAATGTGGAT TCATGGTGTA AAGCCTGTGG TGAGGTGGAG 
CTTCCCTCAG AACTGCAAGA TTTAGAAGAT GCTATCCATC ATCATCAGGG AATATATGAG 
CATATTACCT TGGCTTACTC TGAGGTAAGC CAAGATGGAA AGTCACTGCT TGACAAACTT 
CAGAGACCTT TGACTCCTGG AAGTTCCGAT TCCCTCACTG CTTCTGCCAA CTACTCAAAA 
GCAGTTCATC ACGTTCTGGA TGTCATCCAT GAAGTACTCC ACCACCAGCG CCAACTGGAA 
AATATTTGGC AGCATCGAAA GGTTCGCTTG CACCAGCGGC TTCAGCTGTG TGTTTTTCAA 
CAGGATGTTC AACAGGTATT GGATTGGATT GAAAACCATG GAGAAGCCTT TCTTAGCAAA 
CATACTGGAG TGGGAAAATC CCTGCATCGT GCTAGAGCAT TACAGAAACG CCATGAAGAC 
TTTGAAGAAG TTGCACAGAA CACATACACC AACGCAGATA AACTTCTAGA AGCAGCCGAA 
CAGCTTGCTC AGACCGGAGA GTGTGATCCA GAGGAAATAT ATCAAGCTGC CCATCAGCTC 
GAAGACAGGA TACAGGATTT TGTCAGACGC GTGGAGCAGC GCAAGATCCT GCTGGACATG 
TCCGTTTCCT TTCACACACA CGTCAAGGAG CTGTGGACAT GGCTTGAAGA GTTGCAGAAA 
GAACTGCTTG ATGATGTGTA TGCAGAGTCT GTGGAGGCCG TCCAAGACCT GATTAAACGT 
TTTGGTCAGC AGCAGCAGAC CACTCTGCAG GTTACAGTCA ATGTCATAAA AGAAGGCGAA 
GATCTCATAC AGCAACTAAG GGATTCGGCC ATTTCTAGTA ACAAGACTCC CCATAACAGC 
TCGATCAACC ACATAGAGAC AGTCCTGCAG CAGCTGGATG AGGCCCAATC TCAGATGGAG 
GAGCTGTTTC AGGAACGCAA GATCAAGCTT GAGCTGTTTC TGCAGCTCCG CATATTTGAA 
AGAGATGCAA TAGATATAAT TTCAGACCTC GAGTCTTGGA ATGATGAGCT CTCTCAACAG 
ATGAATGACT TTGACACTGA AGATCTTACA ATTGCAGAAC AAAGGCTCCA GCATCATGCA 
GACAAAGCCC TGACCATGAA TAATTTGACC TTTGATGTCA TCCACCAAGG ACAGGATCTG 
CTGCAATATG TCAACGAAGT GCAGGCATCT GGTGTTGAGC TACTTTGTGA CAGGGATGTA 
GACATGGCAA CTCGTGTTCA GGATCTGCTG GAGTTTCTCC ATGAGAAGCA GCAGGAGCTG 
GATGTAGCAG CCGAGCAGCA CCGCAAGCAC TTGGAGCAGT GCGTGCAGCT GCGCCATCTG 
CAGGCGGAGG TCAAGCAGGT CTTGGGCTGG ATCCGCAATG GGGAGTCAAT GTTAAACGCG 
GGCCTTATCA CTGCTAGCTC TTTGCAAGAG GCAGAACAGT TACAGAGGGA ACATGAGCAG 
TTTCAACATG CAATAGAGAA AACACATCAA AGTGCCCTTC AGGTTCAGCA GAAAGCAGAG 
GCAATGCTAC AGGCTAATCA CTATGACATG GATATGATCA GGGAGTGTGC AGAGAAGGTT 
GCTTCCCATT GGCAACAACT GATGCTCAAG ATGGAGGACC GTCTCAAGCT TGTGAATGCC 
TCTGTTGCCT TCTATAAAAC TTCTGAACAG GTGTGCAGTG TATTGGAAAG CCTGGAGCAA 
GAGTATAAGC GGGAAGAAGA CTGGTGTGGG GGAGCAGACA AGCTGGGTCC CAACTCTGAA 
ACAGATCACG TTACCCCCAT GATAAGCAAA CACTTGGAAC AGAAGGAGGC ATTCCTAAAG 
GCTTGCACGC TGGCCCGGAG GAATGCTGAT GTCTTCCTGA AATACCTGCA CAGGAATAGC 
GTAAACATGC CAGGGATGGT GACGCACATC AAAGCACCTG AACAGCAAGT CAAAAATATC 
TTGAATGAAC TCTTCCAGCG GGAGAACCGT GTGCTGCATT ATTGGACCAT GCGTAAAAGA 
CGTCTTGATC AATGCCAACA GTACGTGGTG TTTGAAAGAA GTGCCAAACA GGCTCTGGAA 
TGGATTCATG ACAATGGGGA GTTTTATTTA TCCACACATA CTTCCACTGG TTCCAGTATC 
CAGCATACTC AAGAGCTGTT AAAAGAACAT GAAGAATTTC AGATCACAGC AAAGCAAACC 
AAAGAGAGGG TGAAGTTGTT GATACAGCTG GCCGATGGCT TTTGTGAAAA AGGGCATGCT 
CATGCAACAG AGATTAAAAA ATGTGTCACG GCAGTGGATA AAAGGTACAG AGACTTTTCC 
CTGCGCATGG AGAAATACAG GACCTCTTTA GAGAAAGCTC TGGGTATTTC CTCTGATTCC 
AATAAATCGA GCAAAAGCTT GCAGCTGGAT ATAATCCCAG CCAGTGTCCC TGGGTCAGAA 
GTGAAACTGC GTGATGCTGC TCATGAGCTT AATGAAGAAA AACGAAAATC TGCCCGCAGG 
AAAGAGTTTA TTATGGCTGA GCTAATTCAG ACAGAAAAGG CTTACGTAAG AGACCTCCGA 
GAATGCATGG ATACATATCT ATGGGAAATG ACAAGTGGAG TTGAGGAGAT CCCACCTGGT 
ATTGTAAACA AAGAGCATAT TATCTTTGGA AACATGCAGG AAATCTATGA GTTCCACAAT 
AATATATTCC TCAAGGAGCT AGAAAAATAT GAACAGTTAC CAGAAGATGT TGGACACTGC 
TTTGTGACAT GGGCAGACAA GTTCCAGATG TATGTTACTT ACTGCAAAAA TAAGCCAGAT 
TCTACCCAGC TAATATTAGA ACATGCAGGA GCATACTTTG ATGAGATACA ACAGCGACAT 
GGACTGGCCA ACTCTATCTC TTCTTACCTT ATCAAACCTG TTCAGAGGAT AACAAAATAT 
CAACTTCTTT TAAAGGAGTT GCTCACATGC TGTGAAGAAG GTAAAGGTGA GATAAAAGAT 
GGCCTGGAGG TGATGCTTAG TGTGCCAAAG CGAGCCAATG ATGCCATGCA CCTCAGTATG 
TTGGAAGGCT TTGATGAAAA TATAGAATCT CAGGGAGAGC TCATCCTTCA AGAATCCTTC 
CAAGTGTGGG ACCCCAAAAC TCTAATTCGA AAGGGAAGAG AGAGGCACCT TTTCCTTTTT 
GAGATGTCCT TGGTTTTCAG TAAAGAAGTA AAGGACTCAA GTGGAAGGAG CAAGTACATT 
TACAAGAGTA AACTGTTTAC TTCTGAATTG GGTGTCACAG AACACGTTGA AGGAGATCCC 
TGCAAGTTTG CTCTGTGGGT TGGAAGAACA CCAACTTCAG ACAACAAAAT TGTTCTCAAG 
GCATCCAGTA TAGAGAATAA ACAGGACTGG ATTAAACACA TCCGGGAAGT GATACAAGAA 
AGGACCATTC ATCTGAAAGG AGCATTGAAA GAGCCAATCC ACATCCCGAA AACAGCACCA 
ACAACAAAAC AGAAAGGAAG AAGAGATGGC GAGGACCTGG ACAGTCAAGG AGATGGTAGC 
AGCCAACCTG ATACTATTTC AATTGCCTCA CGAACTTCAC AGAACACACT TGACAGTGAT 
AAGTTGTCTG GTGGGTGCGA GCTGACGGTG GTAATCCATG ATTTCACCGC CTGCAACAGT 
AATGAGCTGA CAATCCGCCG TGGGCAGACT GTGGAGGTCC TGGAGCGACC CCATGACAAA 
CCCGACTGGT GTCTGGTTCG AACTACAGAT CGCTCCCCAG CTGCAGAAGG CCTGGTCCCC 
TGTGGGTCCC TCTGCATTGC TCACTCTCGC AGTAGTATGG AGATGGAGGG GATCTTTAAC 
CACAAAGACT CCCTTTCAGT CTCCAGCAAT GATGCCAGTC CTCCTGCTTC AGTAACATCT 
CTTCAACCCC ATATGATCGG GGCACAGAGC TCCCCGGGTC CCAAGCGCCC TGGCAACACT 
TTGAGAAAAT GGCTTACCAG TCCTGTGAGG CGTCTCAGCA GCGGGAAAGC AGATGGGCAC 
GTCAAAAAAC TGGCCCACAA GCACAAGAAG AGTAGAGAGG TTCGCAAAAG TGCTGATGCT 
GGTTCTCAGA AGGACTCTGA TGATAGTGCA GCAACCCCAC AAGATGAGAC TATTGAAGAG 
AGGGGTCGGA ACGAGGGGCT GAGCAGTGGC ACTCTCTCGA AGTCATCCTC TTCAGGCATG 
CAGAGCTGTG GAGAGGAAGA AGGTGAAGAG GGAGCAGATG CTGTACCACT TCCTCCTCCC 
ATGGCAATTC AGCAACACAG TCTTCTCCAG CCTGATTCAC AGGATGACAA GACCTCTTCT 
CGATTATTGG TGCGGCCTAC CAGCTCTGAG ACGCCCAGTG CTGCAGAACT AGTCAGTGCA 
ATTGAAGAGC TCGTCAAAAG CAAAATGGCC TTGGAAGACC GTCCAAGTTC CTTGTTAGTA 
GATCAAGGTG ACAGCAGCAG TCCATCCTTC AACCCTTCAG ATAACTCTCT TCTCTCCTCA 
TCATCACCTA TAGATGAGAT GGAAGAAAGG AAGTCCAGCT CCTTAAAAAG ACGACACTAT 
GTCTTGCAGG AATTAGTGGA GACAGAGCGT GATTACGTAC GAGATCTGGG CTATGTAGTT 
GAGGGTTATA TGGCACTTAT GAAGGAAGAT GGTGTTCCTG ATGATATGAA AGGCAAAGAC 
AAGATTGTAT TTGGAAATAT TCACCAGATT TATGACTGGC ACAGAGACTT CTTTTTAGGA 
GAGTTGGAGA AGTGCCTTGA AGATCCAGAA AAGCTGGGAT CCCTGTTTGT TAAGCATGAG 
AGAAGGTTGC ACATGTACAT AGTTTACTGC CAGAACAAAC CAAAGTCTGA GCACATTGTC 
TCAGAATACA TTGACACATT TTTTGAGGAT CTAAAGCAAC GTCTGGGCCA CAGACTTCAG 
CTCACAGACT TGTTAATAAA ACCTGTGCAG AGAATTATGA AATACCAGCT ATTACTAAAG 
GATTTCCTCA AATATTCTAA AAAAGCTAAC CTTGATACAA CAGAACTAGA GAGAGCTGTA 
GAGGTCATGT GTATAGTACC AAAACGGTGT AATGACATGA TGAATGTTGG CCGCCTGCAA 
GGATTTGATG GTAAAATTGT AGCCCAGGGT AAGCTGCTAC TCCAGGACAC ATTCTTGGTT 
ACGGACCAGG ACGCAGGTCT TCTGCCACGC TGCAAGGAGA GACGAGTCTT CCTGTTTGAG 
CAGATTGTTA TTTTCAGTGA GCCGCTAGAT AAAAAGAAAG GTTTCTCCAT GCCCGGATTC 
TTGTTTAAAA ACAGTATCAA GGTGAGTTGC CTTTCCCTGG AGGAAAATGT GGACAATGAT 
CCATGTAAAT TTGCACTAAC ATCAAGAACG GGTGATGTCA CGGAGACCTT TGTTTTGCAT 
TCTGCCAGTC CAGGAGTCCG CCAGCTGTGG ATCCATGAAA TTAACCAAAT TTTGGAAAAC 
CAGCGCAACT TTTTAAATGC CTTGACGTCT CCAATCGAGT ACCAGAGGAA CCACAGCAGT 
GGTGGAGGTG GCAGCAGCAG CAGCAGTGGT AACAGCAGCG GCCCCAGCAG CTGTAGCGGA 
ATCCCCAGCT CCAGCCGTAG CCGGCCATCC CGGATCCCCC AGCCTGTCAG ACACCATTCC 
CCAGTCCTGG TCTCCTCTGC AGCCTCGGCC CAAGCAGAGG CAGACAAGAT GTCAGGTATG 
TCCATTCACA ATCTCTCCCT GCCACACTAC AACACCTCCT TAGAAACTGG CCTGAGCCAG 
CCAGACAGGC ACCCTCCCAG TGCAGAACCG GACGGTCCTC AGAGAGAAGC TGAGCAGATT 
CCCAAGATGA AAGTTATTGA GAGTCCCAGG AAGACAGCGG GAAACATTTC TGGCTCAGCT 
GACGGAGCCC AGAAAGACTC ACGTGGAAGC TCAGAGGAAA GTCGCTCAAG AAACAGCATA 
GGATCACTGA CGCTGGGGAA GCCAAGGCCA GGAGCAATCT CACCAATGAA CTCTCCTCTC 
TCCACGACTT TCCCTTCTCC TTTTGGCAAA GAAACCTTTC CACCCAGCAG CCCCCTGCAG 
AAGGGCTCCT TTTGGAGCTC CATCCCAGCA TCCCCTGCCA GCCGCCCTGG CTCCTTCACT 
TTCCCTGGGG ACAATGACTC CCTCCAGCGG CAGGTCCACC GCCACTCTTC ACACAGCAAG 
GACACAGACC GCATGAGCAC ATGCTCCTCG ACCAGCGAGC AGTCAGTTCA CTCCACCCAG 
AGTAATGGGA GTGAAAGTAG CAGCAGTAGC AATATCTCCA CCATGCTGGT GACACACGAT 
TACACGGCAG TGAAGGAGGA TGAGATAAAT GTCTACCAAG GAGAGGTTGT GCAGATTCTA 
GCCAGCAACC AACAGAACAT GTTTTTGGTG TTCAGAGCCG CCACTGATCA GTGCCCCGCA 
GCTGAGGGCT GGATTCCCGG CTATGTCTTG GGGCATACCA GTGCAGTCAT CATGGACAAC 
CCTGATGGAA CCATCAAGAA GTCAACGTCT TGGCACACAG CACTCCGTTT AAGGAAGAAA 
TCTGAGAAAA AAGATAAAGA CGGCAAAAGG GAAGGCAAGC TAGAAAATGG TTACCGCAAA 
TCCCGAGAAG GACTTGCCAA CAAGGTTTCT GTAAAGCTTC TCAACCCCAA TTACATTTAT 
GATGTTCCCC CAGAGTTTAT AGTACCATTG AGTGAGGTAA CATGTGAGAC AGGAGAGACC 
ATCGTGCTTA GGTGTAAAGT CTGTGGTCGT CCCAAGGCTT CAGTTACTTG GAAAGGTCCT 
GATCATAACA CACTGAGCAA TGACGGTCAT CACAGCATTT CGTACAGTGA CTTAGGGGAG 
GCTTCACTGA AAATCATTGG CGTCACTGCA GAAGACGATG GTATCTATAC ATGTATAGCT 
GCAAACGATA TGGGTTCAGT TTCATCTTCC GCCAGTCTAC GAGTTTTAGG TCCTGGAAGT 
GATGGAATCA TGGTAATCTG GAAAGACAAC TTTGATTCCC TTTACAGTGA AGTGGCTGAG 
CTTGGCAGGG GCAGATTTTC TGTCGTTAAG AAATGTGACC AGAAGGGAAC CAAGCGAGCA 
GTAGCCACTA AGTTTGTGAA TAAGAAGTTG ATGAAGCGAG ACCAGGTTAC CCATGAACTT 
GGAGTCATGC AGAATCTCCA GCATCCCCAA CTCATTGGCC TTATTGATAC CTTTGAGACC 
GCCACCAACT ACATACTAGT ATTAGAAATG GCTGACCAGG GTCGCCTTCT AGACTACGTT 
GTGCGATGGG GAAACCTCAC TGAAGGGAAG ATCAGACTCT ACCTGGGAGA GATTCTGGAA 
GCTGTGCAGT ATCTTCACAA CTGCAGAATA GCACATTTGG ACCTAAAGCC TGAAAATATT 
TTGGTGGACC AGAGTTCCTC TAAGCCAACA ATAAAACTGG CTGACTTTGG AGATGCAGTC 
CAGCTCAATA CCACATATTA TATCCACCAG TTACTTGGAA ACCCAGAGTT TGCAGCTCCT 
GAAATTATTC TTGGAAATCC TGTTTCCCTC ACTTCAGATG TTTGGAGCAT TGGAGTGCTC 
ACGTATGTCC TTCTTAGTGG CGTCTCTCCT TTCCTGGATG AAAGCGTAGA GGAAACTTGC 
CTGAATATTT GCCGCTTAGA CTTTAGTTTC CCAGATGACT ACTTCAAAGG AGTTAGCCAG 
AAGGCCAAAG ATTTTGTATG TTTCCTGCTT CAGGATGACC CAGCTAAGCG TCCCTCAGCT 
GCACTGACCC TCCAGGAGCA ATGGCTGCAG CTGGGGAATA GCAAAGGCGC TGACAGCATT 
GACATATCCA GACTGACTTC GTTCATTGAG CGGCGAAAAC ACCAGAATGA TGTTCGACCT 
ATCCGTAGCA TTAAAAACTT CTTACAGAGC AGGCTCTTGC CAAGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137712.1
CDS	399..9629
Translation	MSSAATTASTAAASTAGSGGGEGAEEAAKDSADIAAFFRSGFRKNDEMKAMEVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILSKKEMPQDLEGARNMIEEHSQLKKKVIKAPIEDLDVEGQKLLQRIQSSDSFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEQYMSNVDSWCKACGEVELPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDVAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRECAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHATEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKEHIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGAYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYIYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPTTKQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVTSLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETIEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKTSSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKANLDTTELERAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLSLEENVDNDPCKFALTSRTGDVTETFVLHSASPGVRQLWIHEINQILENQRNFLNALTSPIEYQRNHSSGGGGSSSSSGNSSGPSSCSGIPSSSRSRPSRIPQPVRHHSPVLVSSAASAQAEADKMSGMSIHNLSLPHYNTSLETGLSQPDRHPPSAEPDGPQREAEQIPKMKVIESPRKTAGNISGSADGAQKDSRGSSEESRSRNSIGSLTLGKPRPGAISPMNSPLSTTFPSPFGKETFPPSSPLQKGSFWSSIPASPASRPGSFTFPGDNDSLQRQVHRHSSHSKDTDRMSTCSSTSEQSVHSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGYVLGHTSAVIMDNPDGTIKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLANKVSVKLLNPNYIYDVPPEFIVPLSEVTCETGETIVLRCKVCGRPKASVTWKGPDHNTLSNDGHHSISYSDLGEASLKIIGVTAEDDGIYTCIAANDMGSVSSSASLRVLGPGSDGIMVIWKDNFDSLYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGVMQNLQHPQLIGLIDTFETATNYILVLEMADQGRLLDYVVRWGNLTEGKIRLYLGEILEAVQYLHNCRIAHLDLKPENILVDQSSSKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDVWSIGVLTYVLLSGVSPFLDESVEETCLNICRLDFSFPDDYFKGVSQKAKDFVCFLLQDDPAKRPSAALTLQEQWLQLGNSKGADSIDISRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_015282226.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282226.2

ORF Insert Sequence:

ATGAGCAGCG CCGCCACCAC CGCCTCCACC GCCGCGGCCA GCACGGCCGG CAGCGGCGGG 
GGAGAAGGGG CCGAGGAGGC GGCCAAGGAC TCGGCGGACA TCGCGGCGTT CTTCCGATCT 
GGGTTTCGCA AAAATGATGA AATGAAGGCT ATGGAAGTTT TGCCAATTTT AAAGGAAAAA 
GTTGCATACC TTTCAGGTGG CAGAGATAAG CGTGGAGGCC CCATTCTAAC GTTTCCAGCA 
CGTAGTAATC ATGATAGAAT ACGGCAAGAA GACCTTAGGA GACTAATTTC ATACCTAGCT 
TGTATTCCAA GTGAGGAAGT ATGTAAAAGA GGATTCACTG TCATTGTAGA TATGCGTGGG 
TCAAAATGGG ACTCTATAAA GCCTCTGCTG AAGATTTTAC AGGAATCCTT CCCATGTTGT 
ATTCATGTTG CACTGATAAT CAAGCCAGAC AATTTCTGGC AGAAACAGAG GACTAACTTT 
GGCAGCTCTA AGTTTGAGTT TGAGACAAAT ATGGTGTCTC TGGAAGGCCT TACCAAAGTA 
GTTGATCCTT CTCAGCTCAC CCCAGAATTT GATGGCTGTT TGGAGTACAA CCATGAGGAG 
TGGATAGAAA TCAGAGTTGC CTTTGAGGAC TATATTAGCA ATGCAACCCA TATGCTGTCC 
AGACTGGAGG AACTGCAAGA TATATTGTCA AAAAAGGAAA TGCCTCAGGA CTTGGAAGGT 
GCTCGCAATA TGATAGAGGA ACATTCACAG TTAAAAAAGA AAGTCATTAA GGCCCCTATT 
GAGGACCTTG ATGTGGAAGG ACAAAAGTTG CTCCAGCGAA TACAGAGCAG CGATAGCTTT 
CCCAAAAAGA ACTCTGGGTC AGGGAATGCA GACTTACAGA ACCTTTTACC CAAAGTATCC 
ACCATGCTGG ACAGGCTGCA CTCAACACGG CAGCATCTGC ATCAGATGTG GCATGTGCGG 
AAATTGAAAT TGGACCAATG TTTTCAGCTC AGGCTGTTTG AGCAGGATGC TGAAAAGATG 
TTTGACTGGA TCACACACAA CAAAGGTCTG TTTCTGAACA GCTACACAGA AATAGGAACC 
AGTCATCCCC ATGCTATGGA ACTTCAGACA CAGCACAATC ACTTTGCCAT GAACTGTATG 
AATGTGTACG TAAACATAAA TCGCATCATG TCAGTAGCAA ATCGTCTGGT TGAGTCTGGC 
CATTATGCTT CACAGCAAAT AAAACAGATT GCTAGTCAAT TGGAGCAAGA ATGGAAAGCA 
TTTGCTGCAG CCTTGGATGA ACGTAGCACG TTACTGGATA TGTCCTCCAT CTTCCATCAG 
AAGGCTGAAC AGTACATGAG CAATGTGGAT TCATGGTGTA AAGCCTGTGG TGAGGTGGAG 
CTTCCCTCAG AACTGCAAGA TTTAGAAGAT GCTATCCATC ATCATCAGGG AATATATGAG 
CATATTACCT TGGCTTACTC TGAGGTAAGC CAAGATGGAA AGTCACTGCT TGACAAACTT 
CAGAGACCTT TGACTCCTGG AAGTTCCGAT TCCCTCACTG CTTCTGCCAA CTACTCAAAA 
GCAGTTCATC ACGTTCTGGA TGTCATCCAT GAAGTACTCC ACCACCAGCG CCAACTGGAA 
AATATTTGGC AGCATCGAAA GGTTCGCTTG CACCAGCGGC TTCAGCTGTG TGTTTTTCAA 
CAGGATGTTC AACAGGTATT GGATTGGATT GAAAACCATG GAGAAGCCTT TCTTAGCAAA 
CATACTGGAG TGGGAAAATC CCTGCATCGT GCTAGAGCAT TACAGAAACG CCATGAAGAC 
TTTGAAGAAG TTGCACAGAA CACATACACC AACGCAGATA AACTTCTAGA AGCAGCCGAA 
CAGCTTGCTC AGACCGGAGA GTGTGATCCA GAGGAAATAT ATCAAGCTGC CCATCAGCTC 
GAAGACAGGA TACAGGATTT TGTCAGACGC GTGGAGCAGC GCAAGATCCT GCTGGACATG 
TCCGTTTCCT TTCACACACA CGTCAAGGAG CTGTGGACAT GGCTTGAAGA GTTGCAGAAA 
GAACTGCTTG ATGATGTGTA TGCAGAGTCT GTGGAGGCCG TCCAAGACCT GATTAAACGT 
TTTGGTCAGC AGCAGCAGAC CACTCTGCAG GTTACAGTCA ATGTCATAAA AGAAGGCGAA 
GATCTCATAC AGCAACTAAG GGATTCGGCC ATTTCTAGTA ACAAGACTCC CCATAACAGC 
TCGATCAACC ACATAGAGAC AGTCCTGCAG CAGCTGGATG AGGCCCAATC TCAGATGGAG 
GAGCTGTTTC AGGAACGCAA GATCAAGCTT GAGCTGTTTC TGCAGCTCCG CATATTTGAA 
AGAGATGCAA TAGATATAAT TTCAGACCTC GAGTCTTGGA ATGATGAGCT CTCTCAACAG 
ATGAATGACT TTGACACTGA AGATCTTACA ATTGCAGAAC AAAGGCTCCA GCATCATGCA 
GACAAAGCCC TGACCATGAA TAATTTGACC TTTGATGTCA TCCACCAAGG ACAGGATCTG 
CTGCAATATG TCAACGAAGT GCAGGCATCT GGTGTTGAGC TACTTTGTGA CAGGGATGTA 
GACATGGCAA CTCGTGTTCA GGATCTGCTG GAGTTTCTCC ATGAGAAGCA GCAGGAGCTG 
GATGTAGCAG CCGAGCAGCA CCGCAAGCAC TTGGAGCAGT GCGTGCAGCT GCGCCATCTG 
CAGGCGGAGG TCAAGCAGGT CTTGGGCTGG ATCCGCAATG GGGAGTCAAT GTTAAACGCG 
GGCCTTATCA CTGCTAGCTC TTTGCAAGAG GCAGAACAGT TACAGAGGGA ACATGAGCAG 
TTTCAACATG CAATAGAGAA AACACATCAA AGTGCCCTTC AGGTTCAGCA GAAAGCAGAG 
GCAATGCTAC AGGCTAATCA CTATGACATG GATATGATCA GGGAGTGTGC AGAGAAGGTT 
GCTTCCCATT GGCAACAACT GATGCTCAAG ATGGAGGACC GTCTCAAGCT TGTGAATGCC 
TCTGTTGCCT TCTATAAAAC TTCTGAACAG GTGTGCAGTG TATTGGAAAG CCTGGAGCAA 
GAGTATAAGC GGGAAGAAGA CTGGTGTGGG GGAGCAGACA AGCTGGGTCC CAACTCTGAA 
ACAGATCACG TTACCCCCAT GATAAGCAAA CACTTGGAAC AGAAGGAGGC ATTCCTAAAG 
GCTTGCACGC TGGCCCGGAG GAATGCTGAT GTCTTCCTGA AATACCTGCA CAGGAATAGC 
GTAAACATGC CAGGGATGGT GACGCACATC AAAGCACCTG AACAGCAAGT CAAAAATATC 
TTGAATGAAC TCTTCCAGCG GGAGAACCGT GTGCTGCATT ATTGGACCAT GCGTAAAAGA 
CGTCTTGATC AATGCCAACA GTACGTGGTG TTTGAAAGAA GTGCCAAACA GGCTCTGGAA 
TGGATTCATG ACAATGGGGA GTTTTATTTA TCCACACATA CTTCCACTGG TTCCAGTATC 
CAGCATACTC AAGAGCTGTT AAAAGAACAT GAAGAATTTC AGATCACAGC AAAGCAAACC 
AAAGAGAGGG TGAAGTTGTT GATACAGCTG GCCGATGGCT TTTGTGAAAA AGGGCATGCT 
CATGCAACAG AGATTAAAAA ATGTGTCACG GCAGTGGATA AAAGGTACAG AGACTTTTCC 
CTGCGCATGG AGAAATACAG GACCTCTTTA GAGAAAGCTC TGGGTATTTC CTCTGATTCC 
AATAAATCGA GCAAAAGCTT GCAGCTGGAT ATAATCCCAG CCAGTGTCCC TGGGTCAGAA 
GTGAAACTGC GTGATGCTGC TCATGAGCTT AATGAAGAAA AACGAAAATC TGCCCGCAGG 
AAAGAGTTTA TTATGGCTGA GCTAATTCAG ACAGAAAAGG CTTACGTAAG AGACCTCCGA 
GAATGCATGG ATACATATCT ATGGGAAATG ACAAGTGGAG TTGAGGAGAT CCCACCTGGT 
ATTGTAAACA AAGAGCATAT TATCTTTGGA AACATGCAGG AAATCTATGA GTTCCACAAT 
AATATATTCC TCAAGGAGCT AGAAAAATAT GAACAGTTAC CAGAAGATGT TGGACACTGC 
TTTGTGACAT GGGCAGACAA GTTCCAGATG TATGTTACTT ACTGCAAAAA TAAGCCAGAT 
TCTACCCAGC TAATATTAGA ACATGCAGGA GCATACTTTG ATGAGATACA ACAGCGACAT 
GGACTGGCCA ACTCTATCTC TTCTTACCTT ATCAAACCTG TTCAGAGGAT AACAAAATAT 
CAACTTCTTT TAAAGGAGTT GCTCACATGC TGTGAAGAAG GTAAAGGTGA GATAAAAGAT 
GGCCTGGAGG TGATGCTTAG TGTGCCAAAG CGAGCCAATG ATGCCATGCA CCTCAGTATG 
TTGGAAGGCT TTGATGAAAA TATAGAATCT CAGGGAGAGC TCATCCTTCA AGAATCCTTC 
CAAGTGTGGG ACCCCAAAAC TCTAATTCGA AAGGGAAGAG AGAGGCACCT TTTCCTTTTT 
GAGATGTCCT TGGTTTTCAG TAAAGAAGTA AAGGACTCAA GTGGAAGGAG CAAGTACATT 
TACAAGAGTA AACTGTTTAC TTCTGAATTG GGTGTCACAG AACACGTTGA AGGAGATCCC 
TGCAAGTTTG CTCTGTGGGT TGGAAGAACA CCAACTTCAG ACAACAAAAT TGTTCTCAAG 
GCATCCAGTA TAGAGAATAA ACAGGACTGG ATTAAACACA TCCGGGAAGT GATACAAGAA 
AGGACCATTC ATCTGAAAGG AGCATTGAAA GAGCCAATCC ACATCCCGAA AACAGCACCA 
ACAACAAAAC AGAAAGGAAG AAGAGATGGC GAGGACCTGG ACAGTCAAGG AGATGGTAGC 
AGCCAACCTG ATACTATTTC AATTGCCTCA CGAACTTCAC AGAACACACT TGACAGTGAT 
AAGTTGTCTG GTGGGTGCGA GCTGACGGTG GTAATCCATG ATTTCACCGC CTGCAACAGT 
AATGAGCTGA CAATCCGCCG TGGGCAGACT GTGGAGGTCC TGGAGCGACC CCATGACAAA 
CCCGACTGGT GTCTGGTTCG AACTACAGAT CGCTCCCCAG CTGCAGAAGG CCTGGTCCCC 
TGTGGGTCCC TCTGCATTGC TCACTCTCGC AGTAGTATGG AGATGGAGGG GATCTTTAAC 
CACAAAGACT CCCTTTCAGT CTCCAGCAAT GATGCCAGTC CTCCTGCTTC AGTAACATCT 
CTTCAACCCC ATATGATCGG GGCACAGAGC TCCCCGGGTC CCAAGCGCCC TGGCAACACT 
TTGAGAAAAT GGCTTACCAG TCCTGTGAGG CGTCTCAGCA GCGGGAAAGC AGATGGGCAC 
GTCAAAAAAC TGGCCCACAA GCACAAGAAG AGTAGAGAGG TTCGCAAAAG TGCTGATGCT 
GGTTCTCAGA AGGACTCTGA TGATAGTGCA GCAACCCCAC AAGATGAGAC TATTGAAGAG 
AGGGGTCGGA ACGAGGGGCT GAGCAGTGGC ACTCTCTCGA AGTCATCCTC TTCAGGCATG 
CAGAGCTGTG GAGAGGAAGA AGGTGAAGAG GGAGCAGATG CTGTACCACT TCCTCCTCCC 
ATGGCAATTC AGCAACACAG TCTTCTCCAG CCTGATTCAC AGGATGACAA GACCTCTTCT 
CGATTATTGG TGCGGCCTAC CAGCTCTGAG ACGCCCAGTG CTGCAGAACT AGTCAGTGCA 
ATTGAAGAGC TCGTCAAAAG CAAAATGGCC TTGGAAGACC GTCCAAGTTC CTTGTTAGTA 
GATCAAGGTG ACAGCAGCAG TCCATCCTTC AACCCTTCAG ATAACTCTCT TCTCTCCTCA 
TCATCACCTA TAGATGAGAT GGAAGAAAGG AAGTCCAGCT CCTTAAAAAG ACGACACTAT 
GTCTTGCAGG AATTAGTGGA GACAGAGCGT GATTACGTAC GAGATCTGGG CTATGTAGTT 
GAGGGTTATA TGGCACTTAT GAAGGAAGAT GGTGTTCCTG ATGATATGAA AGGCAAAGAC 
AAGATTGTAT TTGGAAATAT TCACCAGATT TATGACTGGC ACAGAGACTT CTTTTTAGGA 
GAGTTGGAGA AGTGCCTTGA AGATCCAGAA AAGCTGGGAT CCCTGTTTGT TAAGCATGAG 
AGAAGGTTGC ACATGTACAT AGTTTACTGC CAGAACAAAC CAAAGTCTGA GCACATTGTC 
TCAGAATACA TTGACACATT TTTTGAGGAT CTAAAGCAAC GTCTGGGCCA CAGACTTCAG 
CTCACAGACT TGTTAATAAA ACCTGTGCAG AGAATTATGA AATACCAGCT ATTACTAAAG 
GATTTCCTCA AATATTCTAA AAAAGCTAAC CTTGATACAA CAGAACTAGA GAGAGCTGTA 
GAGGTCATGT GTATAGTACC AAAACGGTGT AATGACATGA TGAATGTTGG CCGCCTGCAA 
GGATTTGATG GTAAAATTGT AGCCCAGGGT AAGCTGCTAC TCCAGGACAC ATTCTTGGTT 
ACGGACCAGG ACGCAGGTCT TCTGCCACGC TGCAAGGAGA GACGAGTCTT CCTGTTTGAG 
CAGATTGTTA TTTTCAGTGA GCCGCTAGAT AAAAAGAAAG GTTTCTCCAT GCCCGGATTC 
TTGTTTAAAA ACAGTATCAA GGTGAGTTGC CTTTCCCTGG AGGAAAATGT GGACAATGAT 
CCATGTAAAT TTGCACTAAC ATCAAGAACG GGTGATGTCA CGGAGACCTT TGTTTTGCAT 
TCTGCCAGTC CAGGAGTCCG CCAGCTGTGG ATCCATGAAA TTAACCAAAT TTTGGAAAAC 
CAGCGCAACT TTTTAAATGC CTTGACGTCT CCAATCGAGT ACCAGAGGAA CCACAGCAGT 
GGTGGAGGTG GCAGCAGCAG CAGCAGTGGT AACAGCAGCG GCCCCAGCAG CTGTAGCGGA 
ATCCCCAGCT CCAGCCGTAG CCGGCCATCC CGGATCCCCC AGCCTGTCAG ACACCATTCC 
CCAGTCCTGG TCTCCTCTGC AGCCTCGGCC CAAGCAGAGG CAGACAAGAT GTCAGGTATG 
TCCATTCACA ATCTCTCCCT GCCACACTAC AACACCTCCT TAGAAACTGG CCTGAGCCAG 
CCAGACAGGC ACCCTCCCAG TGCAGAACCG GACGGTCCTC AGAGAGAAGC TGAGCAGATT 
CCCAAGATGA AAGTTATTGA GAGTCCCAGG AAGACAGCGG GAAACATTTC TGGCTCAGCT 
GACGGAGCCC AGAAAGACTC ACGTGGAAGC TCAGAGGAAA GTCGCTCAAG AAACAGCATA 
GGATCACTGA CGCTGGGGAA GCCAAGGCCA GGAGCAATCT CACCAATGAA CTCTCCTCTC 
TCCACGACTT TCCCTTCTCC TTTTGGCAAA GAAACCTTTC CACCCAGCAG CCCCCTGCAG 
AAGGGCTCCT TTTGGAGCTC CATCCCAGCA TCCCCTGCCA GCCGCCCTGG CTCCTTCACT 
TTCCCTGGGG ACAATGACTC CCTCCAGCGG CAGGTCCACC GCCACTCTTC ACACAGCAAG 
GACACAGACC GCATGAGCAC ATGCTCCTCG ACCAGCGAGC AGTCAGTTCA CTCCACCCAG 
AGTAATGGGA GTGAAAGTAG CAGCAGTAGC AATATCTCCA CCATGCTGGT GACACACGAT 
TACACGGCAG TGAAGGAGGA TGAGATAAAT GTCTACCAAG GAGAGGTTGT GCAGATTCTA 
GCCAGCAACC AACAGAACAT GTTTTTGGTG TTCAGAGCCG CCACTGATCA GTGCCCCGCA 
GCTGAGGGCT GGATTCCCGG CTATGTCTTG GGGCATACCA GTGCAGTCAT CATGGACAAC 
CCTGATGGAA CCATCAAGAA GTCAACGTCT TGGCACACAG CACTCCGTTT AAGGAAGAAA 
TCTGAGAAAA AAGATAAAGA CGGCAAAAGG GAAGGCAAGC TAGAAAATGG TTACCGCAAA 
TCCCGAGAAG GACTTGCCAA CAAGGTTTCT GTAAAGCTTC TCAACCCCAA TTACATTTAT 
GATGTTCCCC CAGAGTTTAT AGTACCATTG AGTGAGGTAA CATGTGAGAC AGGAGAGACC 
ATCGTGCTTA GGTGTAAAGT CTGTGGTCGT CCCAAGGCTT CAGTTACTTG GAAAGGTCCT 
GATCATAACA CACTGAGCAA TGACGGTCAT CACAGCATTT CGTACAGTGA CTTAGGGGAG 
GCTTCACTGA AAATCATTGG CGTCACTGCA GAAGACGATG GTATCTATAC ATGTATAGCT 
GCAAACGATA TGGGTTCAGT TTCATCTTCC GCCAGTCTAC GAGTTTTAGG TCCTGGAAGT 
GATGGAATCA TGGTAATCTG GAAAGACAAC TTTGATTCCC TTTACAGTGA AGTGGCTGAG 
CTTGGCAGGG GCAGATTTTC TGTCGTTAAG AAATGTGACC AGAAGGGAAC CAAGCGAGCA 
GTAGCCACTA AGTTTGTGAA TAAGAAGTTG ATGAAGCGAG ACCAGGTTAC CCATGAACTT 
GGAGTCATGC AGAATCTCCA GCATCCCCAA CTCATTGGCC TTATTGATAC CTTTGAGACC 
GCCACCAACT ACATACTAGT ATTAGAAATG GCTGACCAGG GTCGCCTTCT AGACTACGTT 
GTGCGATGGG GAAACCTCAC TGAAGGGAAG ATCAGACTCT ACCTGGGAGA GATTCTGGAA 
GCTGTGCAGT ATCTTCACAA CTGCAGAATA GCACATTTGG ACCTAAAGCC TGAAAATATT 
TTGGTGGACC AGAGTTCCTC TAAGCCAACA ATAAAACTGG CTGACTTTGG AGATGCAGTC 
CAGCTCAATA CCACATATTA TATCCACCAG TTACTTGGAA ACCCAGAGTT TGCAGCTCCT 
GAAATTATTC TTGGAAATCC TGTTTCCCTC ACTTCAGATG TTTGGAGCAT TGGAGTGCTC 
ACGTATGTCC TTCTTAGTGG CGTCTCTCCT TTCCTGGATG AAAGCGTAGA GGAAACTTGC 
CTGAATATTT GCCGCTTAGA CTTTAGTTTC CCAGATGACT ACTTCAAAGG AGTTAGCCAG 
AAGGCCAAAG ATTTTGTATG TTTCCTGCTT CAGGATGACC CAGCTAAGCG TCCCTCAGCT 
GCACTGACCC TCCAGGAGCA ATGGCTGCAG CTGGGGAATA GCAAAGGCGC TGACAGCATT 
GACATATCCA GACTGACTTC GTTCATTGAG CGGCGAAAAC ACCAGAATGA TGTTCGACCT 
ATCCGTAGCA TTAAAAACTT CTTACAGAGC AGGCTCTTGC CAAGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_015282226.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137712.1 Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), mRNA.

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CloneID	OGa32204
Clone ID Related Accession (Same CDS sequence)	XM_015282226.1
Accession Version	XM_015282226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	triple functional domain protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455784.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCG CCGCCACCAC CGCCTCCACC GCCGCGGCCA GCACGGCCGG CAGCGGCGGG 
GGAGAAGGGG CCGAGGAGGC GGCCAAGGAC TCGGCGGACA TCGCGGCGTT CTTCCGATCT 
GGGTTTCGCA AAAATGATGA AATGAAGGCT ATGGAAGTTT TGCCAATTTT AAAGGAAAAA 
GTTGCATACC TTTCAGGTGG CAGAGATAAG CGTGGAGGCC CCATTCTAAC GTTTCCAGCA 
CGTAGTAATC ATGATAGAAT ACGGCAAGAA GACCTTAGGA GACTAATTTC ATACCTAGCT 
TGTATTCCAA GTGAGGAAGT ATGTAAAAGA GGATTCACTG TCATTGTAGA TATGCGTGGG 
TCAAAATGGG ACTCTATAAA GCCTCTGCTG AAGATTTTAC AGGAATCCTT CCCATGTTGT 
ATTCATGTTG CACTGATAAT CAAGCCAGAC AATTTCTGGC AGAAACAGAG GACTAACTTT 
GGCAGCTCTA AGTTTGAGTT TGAGACAAAT ATGGTGTCTC TGGAAGGCCT TACCAAAGTA 
GTTGATCCTT CTCAGCTCAC CCCAGAATTT GATGGCTGTT TGGAGTACAA CCATGAGGAG 
TGGATAGAAA TCAGAGTTGC CTTTGAGGAC TATATTAGCA ATGCAACCCA TATGCTGTCC 
AGACTGGAGG AACTGCAAGA TATATTGTCA AAAAAGGAGA TGCCTCAGGA CTTGGAAGGT 
GCTCGCAATA TGATAGAGGA ACATTCACAG TTAAAAAAGA AAGTCATTAA GGCCCCTATT 
GAGGACCTTG ATGTGGAAGG ACAAAAGTTG CTCCAGCGAA TACAGAGCAG CGATAGCTTT 
CCCAAAAAGA ACTCTGGGTC AGGGAATGCA GACTTACAGA ACCTTTTACC CAAAGTATCC 
ACCATGCTGG ACAGGCTGCA CTCAACACGG CAGCATCTGC ATCAGATGTG GCATGTGCGG 
AAATTGAAAT TGGACCAATG TTTTCAGCTC AGGCTGTTTG AGCAGGATGC TGAAAAGATG 
TTTGACTGGA TCACACACAA CAAAGGTCTG TTTCTGAACA GCTACACAGA AATAGGAACC 
AGTCATCCCC ATGCTATGGA ACTTCAGACA CAGCACAATC ACTTTGCCAT GAACTGTATG 
AATGTGTACG TAAACATAAA TCGCATCATG TCAGTAGCAA ATCGTCTGGT TGAGTCTGGC 
CATTATGCTT CACAGCAAAT AAAACAGATT GCTAGTCAAT TGGAGCAAGA ATGGAAAGCA 
TTTGCTGCAG CCTTGGATGA ACGTAGCACG TTACTGGATA TGTCCTCCAT CTTCCATCAG 
AAGGCTGAAC AGTACATGAG CAATGTGGAT TCATGGTGTA AAGCCTGTGG TGAGGTGGAG 
CTTCCCTCAG AACTGCAAGA TTTAGAAGAT GCTATCCATC ATCATCAGGG AATATATGAG 
CATATTACCT TGGCTTACTC TGAGGTAAGC CAAGATGGAA AGTCACTGCT TGACAAACTT 
CAGAGACCTT TGACTCCTGG AAGTTCCGAT TCCCTCACTG CTTCTGCCAA CTACTCAAAA 
GCAGTTCATC ACGTTCTGGA TGTCATCCAT GAAGTACTCC ACCACCAGCG CCAACTGGAA 
AATATTTGGC AGCATCGAAA GGTTCGCTTG CACCAGCGGC TTCAGCTGTG TGTTTTTCAA 
CAGGATGTTC AACAGGTATT GGATTGGATT GAAAACCATG GAGAAGCCTT TCTTAGCAAA 
CATACTGGAG TGGGAAAATC CCTGCATCGT GCTAGAGCAT TACAGAAACG CCATGAAGAC 
TTTGAAGAAG TTGCACAGAA CACATACACC AACGCAGATA AACTTCTAGA AGCAGCCGAA 
CAGCTTGCTC AGACCGGAGA GTGTGATCCA GAGGAAATAT ATCAAGCTGC CCATCAGCTC 
GAAGACAGGA TACAGGATTT TGTCAGACGC GTGGAGCAGC GCAAGATCCT GCTGGACATG 
TCCGTTTCCT TTCACACACA CGTCAAGGAG CTGTGGACAT GGCTTGAAGA GTTGCAGAAA 
GAACTGCTTG ATGATGTGTA TGCAGAGTCT GTGGAGGCCG TCCAAGACCT GATTAAACGT 
TTTGGTCAGC AGCAGCAGAC CACTCTGCAG GTTACAGTCA ATGTCATAAA AGAAGGCGAA 
GATCTCATAC AGCAACTAAG GGATTCGGCC ATTTCTAGTA ACAAGACTCC CCATAACAGC 
TCGATCAACC ACATAGAGAC AGTCCTGCAG CAGCTGGATG AGGCCCAATC TCAGATGGAG 
GAGCTGTTTC AGGAACGCAA GATCAAGCTT GAGCTGTTTC TGCAGCTCCG CATATTTGAA 
AGAGATGCAA TAGATATAAT TTCAGACCTC GAGTCTTGGA ATGATGAGCT CTCTCAACAG 
ATGAATGACT TTGACACTGA AGATCTTACA ATTGCAGAAC AAAGGCTCCA GCATCATGCA 
GACAAAGCCC TGACCATGAA TAATTTGACC TTTGATGTCA TCCACCAAGG ACAGGATCTG 
CTGCAATATG TCAACGAAGT GCAGGCATCT GGTGTTGAGC TACTTTGTGA CAGGGATGTA 
GACATGGCAA CTCGTGTTCA GGATCTGCTG GAGTTTCTCC ATGAGAAGCA GCAGGAGCTG 
GATGTAGCAG CCGAGCAGCA CCGCAAGCAC TTGGAGCAGT GCGTGCAGCT GCGCCATCTG 
CAGGCGGAGG TCAAGCAGGT CTTGGGCTGG ATCCGCAATG GGGAGTCAAT GTTAAACGCG 
GGCCTTATCA CTGCTAGCTC TTTGCAAGAG GCAGAACAGT TACAGAGGGA ACATGAGCAG 
TTTCAACATG CAATAGAGAA AACACATCAA AGTGCCCTTC AGGTTCAGCA GAAAGCAGAG 
GCAATGCTAC AGGCTAATCA CTATGACATG GATATGATCA GGGAGTGTGC AGAGAAGGTT 
GCTTCCCATT GGCAACAACT GATGCTCAAG ATGGAGGACC GTCTCAAGCT TGTGAATGCC 
TCTGTTGCCT TCTATAAAAC TTCTGAACAG GTGTGCAGTG TATTGGAAAG CCTGGAGCAA 
GAGTATAAGC GGGAAGAAGA CTGGTGTGGG GGAGCAGACA AGCTGGGTCC CAACTCTGAA 
ACAGATCACG TTACCCCCAT GATAAGCAAA CACTTGGAAC AGAAGGAGGC ATTCCTAAAG 
GCTTGCACGC TGGCCCGGAG GAATGCTGAT GTCTTCCTGA AATACCTGCA CAGGAATAGC 
GTAAACATGC CAGGGATGGT GACGCACATC AAAGCACCTG AACAGCAAGT CAAAAATATC 
TTGAATGAAC TCTTCCAGCG GGAGAACCGT GTGCTGCATT ATTGGACCAT GCGTAAAAGA 
CGTCTTGATC AATGCCAACA GTACGTGGTG TTTGAAAGAA GTGCCAAACA GGCTCTGGAA 
TGGATTCATG ACAATGGGGA GTTTTATTTA TCCACACATA CTTCCACTGG TTCCAGTATC 
CAGCATACTC AAGAGCTGTT AAAAGAACAT GAAGAATTTC AGATCACAGC AAAGCAAACC 
AAAGAGAGGG TGAAGTTGTT GATACAGCTG GCCGATGGCT TTTGTGAAAA AGGGCATGCT 
CATGCAACAG AGATTAAAAA ATGTGTCACG GCAGTGGATA AAAGGTACAG AGACTTTTCC 
CTGCGCATGG AGAAATACAG GACCTCTTTA GAGAAAGCTC TGGGTATTTC CTCTGATTCC 
AATAAATCGA GCAAAAGCTT GCAGCTGGAT ATAATCCCAG CCAGTGTCCC TGGGTCAGAA 
GTGAAACTGC GTGATGCTGC TCATGAGCTT AATGAAGAAA AACGAAAATC TGCCCGCAGG 
AAAGAGTTTA TTATGGCTGA GCTAATTCAG ACAGAAAAGG CTTACGTAAG AGACCTCCGA 
GAATGCATGG ATACATATCT ATGGGAAATG ACAAGTGGAG TTGAGGAGAT CCCACCTGGT 
ATTGTAAACA AAGAGCATAT TATCTTTGGA AACATGCAGG AAATCTATGA GTTCCACAAT 
AATATATTCC TCAAGGAGCT AGAAAAATAT GAACAGTTAC CAGAAGATGT TGGACACTGC 
TTTGTGACAT GGGCAGACAA GTTCCAGATG TATGTTACTT ACTGCAAAAA TAAGCCAGAT 
TCTACCCAGC TAATATTAGA ACATGCAGGA GCATACTTTG ATGAGATACA ACAGCGACAT 
GGACTGGCCA ACTCTATCTC TTCTTACCTT ATCAAACCTG TTCAGAGGAT AACAAAATAT 
CAACTTCTTT TAAAGGAGTT GCTCACATGC TGTGAAGAAG GTAAAGGTGA GATAAAAGAT 
GGCCTGGAGG TGATGCTTAG TGTGCCAAAG CGAGCCAATG ATGCCATGCA CCTCAGTATG 
TTGGAAGGCT TTGATGAAAA TATAGAATCT CAGGGAGAGC TCATCCTTCA AGAATCCTTC 
CAAGTGTGGG ACCCCAAAAC TCTAATTCGA AAGGGAAGAG AGAGGCACCT TTTCCTTTTT 
GAGATGTCCT TGGTTTTCAG TAAAGAAGTA AAGGACTCAA GTGGAAGGAG CAAGTACATT 
TACAAGAGTA AACTGTTTAC TTCTGAATTG GGTGTCACAG AACACGTTGA AGGAGATCCC 
TGCAAGTTTG CTCTGTGGGT TGGAAGAACA CCAACTTCAG ACAACAAAAT TGTTCTCAAG 
GCATCCAGTA TAGAGAATAA ACAGGACTGG ATTAAACACA TCCGGGAAGT GATACAAGAA 
AGGACCATTC ATCTGAAAGG AGCATTGAAA GAGCCAATCC ACATCCCGAA AACAGCACCA 
ACAACAAAAC AGAAAGGAAG AAGAGATGGC GAGGACCTGG ACAGTCAAGG AGATGGTAGC 
AGCCAACCTG ATACTATTTC AATTGCCTCA CGAACTTCAC AGAACACACT TGACAGTGAT 
AAGTTGTCTG GTGGGTGCGA GCTGACGGTG GTAATCCATG ATTTCACCGC CTGCAACAGT 
AATGAGCTGA CAATCCGCCG TGGGCAGACT GTGGAGGTCC TGGAGCGACC CCATGACAAA 
CCCGACTGGT GTCTGGTTCG AACTACAGAT CGCTCCCCAG CTGCAGAAGG CCTGGTCCCC 
TGTGGGTCCC TCTGCATTGC TCACTCTCGC AGTAGTATGG AGATGGAGGG GATCTTTAAC 
CACAAAGACT CCCTTTCAGT CTCCAGCAAT GATGCCAGTC CTCCTGCTTC AGTAACATCT 
CTTCAACCCC ATATGATCGG GGCACAGAGC TCCCCGGGTC CCAAGCGCCC TGGCAACACT 
TTGAGAAAAT GGCTTACCAG TCCTGTGAGG CGTCTCAGCA GCGGGAAAGC AGATGGGCAC 
GTCAAAAAAC TGGCCCACAA GCACAAGAAG AGTAGAGAGG TTCGCAAAAG TGCTGATGCT 
GGTTCTCAGA AGGACTCTGA TGATAGTGCA GCAACCCCAC AAGATGAGAC TATTGAAGAG 
AGGGGTCGGA ACGAGGGGCT GAGCAGTGGC ACTCTCTCGA AGTCATCCTC TTCAGGCATG 
CAGAGCTGTG GAGAGGAAGA AGGTGAAGAG GGAGCAGATG CTGTACCACT TCCTCCTCCC 
ATGGCAATTC AGCAACACAG TCTTCTCCAG CCTGATTCAC AGGATGACAA GACCTCTTCT 
CGATTATTGG TGCGGCCTAC CAGCTCTGAG ACGCCCAGTG CTGCAGAACT AGTCAGTGCA 
ATTGAAGAGC TCGTCAAAAG CAAAATGGCC TTGGAAGACC GTCCAAGTTC CTTGTTAGTA 
GATCAAGGTG ACAGCAGCAG TCCATCCTTC AACCCTTCAG ATAACTCTCT TCTCTCCTCA 
TCATCACCTA TAGATGAGAT GGAAGAAAGG AAGTCCAGCT CCTTAAAAAG ACGACACTAT 
GTCTTGCAGG AATTAGTGGA GACAGAGCGT GATTACGTAC GAGATCTGGG CTATGTAGTT 
GAGGGTTACA TGGCACTTAT GAAGGAAGAT GGTGTTCCTG ATGATATGAA AGGCAAAGAC 
AAGATTGTAT TTGGAAATAT TCACCAGATT TATGACTGGC ACAGAGACTT CTTTTTAGGA 
GAGTTGGAGA AGTGCCTTGA AGATCCAGAA AAGCTGGGAT CCCTGTTTGT TAAGCATGAG 
AGAAGGTTGC ACATGTACAT AGTTTACTGC CAGAACAAAC CAAAGTCTGA GCACATTGTC 
TCAGAATACA TTGACACATT TTTTGAGGAT CTAAAGCAAC GTCTGGGCCA CAGACTTCAG 
CTCACAGACT TGTTAATAAA ACCTGTGCAG AGAATTATGA AATACCAGCT ATTACTAAAG 
GATTTCCTCA AATATTCTAA AAAAGCTAAC CTTGATACAA CAGAACTAGA GAGAGCTGTA 
GAGGTCATGT GTATAGTACC AAAACGGTGT AATGACATGA TGAATGTTGG CCGCCTGCAA 
GGATTTGATG GTAAAATTGT AGCCCAGGGT AAGCTGCTAC TCCAGGACAC ATTCTTGGTT 
ACGGACCAGG ACGCAGGTCT TCTGCCACGC TGCAAGGAGA GACGAGTCTT CCTGTTTGAG 
CAGATTGTTA TTTTCAGTGA GCCGCTAGAT AAAAAGAAAG GTTTCTCCAT GCCCGGATTC 
TTGTTTAAAA ACAGTATCAA GGTGAGTTGC CTTTCCCTGG AGGAAAATGT GGACAATGAT 
CCATGTAAAT TTGCACTAAC ATCAAGAACG GGTGATGTCA CGGAGACCTT TGTTTTGCAT 
TCTGCCAGTC CAGGAGTCCG CCAGCTGTGG ATCCATGAAA TTAACCAAAT TTTGGAAAAC 
CAGCGCAACT TTTTAAATGC CTTGACGTCT CCAATCGAGT ACCAGAGGAA CCACAGCAGT 
GGTGGAGGTG GCAGCAGCAG CAGCAGTGGT AACAGCAGCG GCCCCAGCAG CTGTAGCGGA 
ATCCCCAGCT CCAGCCGTAG CCGGCCATCC CGGATCCCCC AGCCTGTCAG ACACCATTCC 
CCAGTCCTGG TCTCCTCTGC AGCCTCGGCC CAAGCAGAGG CAGACAAGAT GTCAGGTATG 
TCCATTCACA ATCTCTCCCT GCCACACTAC AACACCTCCT TAGAAACTGG CCTGAGCCAG 
CCAGACAGGC ACCCTCCCAG TGCAGAACCG GACGGTCCTC AGAGAGAAGC TGAGCAGATT 
CCCAAGATGA AAGTTATTGA GAGTCCCAGG AAGACAGCGG GAAACATTTC TGGCTCAGCT 
GACGGAGCCC AGAAAGACTC ACGTGGAAGC TCAGAGGAAA GTCGCTCAAG AAACAGCATA 
GGATCACTGA CGCTGGGGAA GCCAAGGCCA GGAGCAATCT CACCAATGAA CTCTCCTCTC 
TCCACGACTT TCCCTTCTCC TTTTGGCAAA GAAACCTTTC CACCCAGCAG CCCCCTGCAG 
AAGGGCTCCT TTTGGAGCTC CATCCCAGCA TCCCCTGCCA GCCGCCCTGG CTCCTTCACT 
TTCCCTGGGG ACAATGACTC CCTCCAGCGG CAGGTCCACC GCCACTCTTC ACACAGCAAG 
GACACAGACC GCATGAGCAC ATGCTCCTCG ACCAGCGAGC AGTCAGTTCA CTCCACCCAG 
AGTAATGGGA GTGAAAGTAG CAGCAGTAGC AATATCTCCA CCATGCTGGT GACACACGAT 
TACACGGCAG TGAAGGAGGA TGAGATAAAT GTCTACCAAG GAGAGGTTGT GCAGATTCTA 
GCCAGCAACC AACAGAACAT GTTTTTGGTG TTCAGAGCCG CCACTGATCA GTGCCCCGCA 
GCTGAGGGCT GGATTCCCGG CTATGTCTTG GGGCATACCA GTGCAGTCAT CATGGACAAC 
CCTGATGGAA CCATCAAGAA GTCAACGTCT TGGCACACAG CACTCCGTTT AAGGAAGAAA 
TCTGAGAAAA AAGATAAAGA CGGCAAAAGG GAAGGCAAGC TAGAAAATGG TTACCGCAAA 
TCCCGAGAAG GACTTGCCAA CAAGGTTTCT GTAAAGCTTC TCAACCCCAA TTACATTTAT 
GATGTTCCCC CAGAGTTTAT AGTACCATTG AGTGAGGTAA CATGTGAGAC AGGAGAGACC 
ATCGTGCTTA GGTGTAAAGT CTGTGGTCGT CCCAAGGCTT CAGTTACTTG GAAAGGTCCT 
GATCATAACA CACTGAGCAA TGACGGTCAT CACAGCATTT CGTACAGTGA CTTAGGGGAG 
GCTTCACTGA AAATCATTGG CGTCACTGCA GAAGACGATG GTATCTATAC ATGTATAGCT 
GCAAACGATA TGGGTTCAGT TTCATCTTCC GCCAGTCTAC GAGTTTTAGG TCCTGGAAGT 
GATGGAATCA TGGTAATCTG GAAAGACAAC TTTGATTCCC TTTACAGTGA AGTGGCTGAG 
CTTGGCAGGG GCAGATTTTC TGTCGTTAAG AAATGTGACC AGAAGGGAAC CAAGCGAGCA 
GTAGCCACTA AGTTTGTGAA TAAGAAGTTG ATGAAGCGAG ACCAGGTTAC CCATGAACTT 
GGAGTCATGC AGAATCTCCA GCATCCCCAG CTCATTGGCC TTATTGATAC CTTTGAGACC 
GCCACCAACT ACATACTAGT ATTAGAAATG GCTGACCAGG GTCGCCTTCT AGACTACGTT 
GTGCGATGGG GAAACCTCAC TGAAGGGAAG ATCAGACTCT ACCTGGGAGA GATTCTGGAA 
GCTGTGCAGT ATCTTCACAA CTGCAGAATA GCACATTTGG ACCTAAAGCC TGAAAATATT 
TTGGTGGACC AGAGTTCCTC TAAGCCAACA ATAAAACTGG CTGACTTTGG AGATGCAGTC 
CAGCTCAATA CCACATATTA TATCCACCAG TTACTTGGAA ACCCAGAGTT TGCAGCTCCT 
GAAATTATTC TTGGAAATCC TGTTTCCCTC ACTTCAGATG TTTGGAGCAT TGGAGTGCTC 
ACGTATGTCC TTCTTAGTGG CGTCTCTCCT TTCCTGGATG AAAGCGTAGA GGAAACTTGC 
CTGAATATTT GCCGCTTAGA CTTTAGTTTC CCAGATGACT ACTTCAAAGG AGTTAGCCAG 
AAGGCCAAAG ATTTTGTATG TTTCCTGCTT CAGGATGACC CAGCTAAGCG TCCCTCAGCT 
GCACTGACCC TCCAGGAGCA ATGGCTGCAG CTGGGGAATA GCAAAGGCGC TGACAGCATT 
GACATATCCA GACTGACTTC GTTCATTGAG CGGCGAAAAC ACCAGAATGA TGTTCGACCT 
ATCCGTAGCA TTAAAAACTT CTTACAGAGC AGGCTCTTGC CAAGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137712.1
CDS	54..9284
Translation	MSSAATTASTAAASTAGSGGGEGAEEAAKDSADIAAFFRSGFRKNDEMKAMEVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILSKKEMPQDLEGARNMIEEHSQLKKKVIKAPIEDLDVEGQKLLQRIQSSDSFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEQYMSNVDSWCKACGEVELPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDVAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRECAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHATEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASVPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKEHIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGAYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYIYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPTTKQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVTSLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETIEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKTSSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKANLDTTELERAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLSLEENVDNDPCKFALTSRTGDVTETFVLHSASPGVRQLWIHEINQILENQRNFLNALTSPIEYQRNHSSGGGGSSSSSGNSSGPSSCSGIPSSSRSRPSRIPQPVRHHSPVLVSSAASAQAEADKMSGMSIHNLSLPHYNTSLETGLSQPDRHPPSAEPDGPQREAEQIPKMKVIESPRKTAGNISGSADGAQKDSRGSSEESRSRNSIGSLTLGKPRPGAISPMNSPLSTTFPSPFGKETFPPSSPLQKGSFWSSIPASPASRPGSFTFPGDNDSLQRQVHRHSSHSKDTDRMSTCSSTSEQSVHSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGYVLGHTSAVIMDNPDGTIKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLANKVSVKLLNPNYIYDVPPEFIVPLSEVTCETGETIVLRCKVCGRPKASVTWKGPDHNTLSNDGHHSISYSDLGEASLKIIGVTAEDDGIYTCIAANDMGSVSSSASLRVLGPGSDGIMVIWKDNFDSLYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGVMQNLQHPQLIGLIDTFETATNYILVLEMADQGRLLDYVVRWGNLTEGKIRLYLGEILEAVQYLHNCRIAHLDLKPENILVDQSSSKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDVWSIGVLTYVLLSGVSPFLDESVEETCLNICRLDFSFPDDYFKGVSQKAKDFVCFLLQDDPAKRPSAALTLQEQWLQLGNSKGADSIDISRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_015282226.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus trio Rho guanine nucleotide exchange factor (TRIO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282226.1

ORF Insert Sequence:

ATGAGCAGCG CCGCCACCAC CGCCTCCACC GCCGCGGCCA GCACGGCCGG CAGCGGCGGG 
GGAGAAGGGG CCGAGGAGGC GGCCAAGGAC TCGGCGGACA TCGCGGCGTT CTTCCGATCT 
GGGTTTCGCA AAAATGATGA AATGAAGGCT ATGGAAGTTT TGCCAATTTT AAAGGAAAAA 
GTTGCATACC TTTCAGGTGG CAGAGATAAG CGTGGAGGCC CCATTCTAAC GTTTCCAGCA 
CGTAGTAATC ATGATAGAAT ACGGCAAGAA GACCTTAGGA GACTAATTTC ATACCTAGCT 
TGTATTCCAA GTGAGGAAGT ATGTAAAAGA GGATTCACTG TCATTGTAGA TATGCGTGGG 
TCAAAATGGG ACTCTATAAA GCCTCTGCTG AAGATTTTAC AGGAATCCTT CCCATGTTGT 
ATTCATGTTG CACTGATAAT CAAGCCAGAC AATTTCTGGC AGAAACAGAG GACTAACTTT 
GGCAGCTCTA AGTTTGAGTT TGAGACAAAT ATGGTGTCTC TGGAAGGCCT TACCAAAGTA 
GTTGATCCTT CTCAGCTCAC CCCAGAATTT GATGGCTGTT TGGAGTACAA CCATGAGGAG 
TGGATAGAAA TCAGAGTTGC CTTTGAGGAC TATATTAGCA ATGCAACCCA TATGCTGTCC 
AGACTGGAGG AACTGCAAGA TATATTGTCA AAAAAGGAGA TGCCTCAGGA CTTGGAAGGT 
GCTCGCAATA TGATAGAGGA ACATTCACAG TTAAAAAAGA AAGTCATTAA GGCCCCTATT 
GAGGACCTTG ATGTGGAAGG ACAAAAGTTG CTCCAGCGAA TACAGAGCAG CGATAGCTTT 
CCCAAAAAGA ACTCTGGGTC AGGGAATGCA GACTTACAGA ACCTTTTACC CAAAGTATCC 
ACCATGCTGG ACAGGCTGCA CTCAACACGG CAGCATCTGC ATCAGATGTG GCATGTGCGG 
AAATTGAAAT TGGACCAATG TTTTCAGCTC AGGCTGTTTG AGCAGGATGC TGAAAAGATG 
TTTGACTGGA TCACACACAA CAAAGGTCTG TTTCTGAACA GCTACACAGA AATAGGAACC 
AGTCATCCCC ATGCTATGGA ACTTCAGACA CAGCACAATC ACTTTGCCAT GAACTGTATG 
AATGTGTACG TAAACATAAA TCGCATCATG TCAGTAGCAA ATCGTCTGGT TGAGTCTGGC 
CATTATGCTT CACAGCAAAT AAAACAGATT GCTAGTCAAT TGGAGCAAGA ATGGAAAGCA 
TTTGCTGCAG CCTTGGATGA ACGTAGCACG TTACTGGATA TGTCCTCCAT CTTCCATCAG 
AAGGCTGAAC AGTACATGAG CAATGTGGAT TCATGGTGTA AAGCCTGTGG TGAGGTGGAG 
CTTCCCTCAG AACTGCAAGA TTTAGAAGAT GCTATCCATC ATCATCAGGG AATATATGAG 
CATATTACCT TGGCTTACTC TGAGGTAAGC CAAGATGGAA AGTCACTGCT TGACAAACTT 
CAGAGACCTT TGACTCCTGG AAGTTCCGAT TCCCTCACTG CTTCTGCCAA CTACTCAAAA 
GCAGTTCATC ACGTTCTGGA TGTCATCCAT GAAGTACTCC ACCACCAGCG CCAACTGGAA 
AATATTTGGC AGCATCGAAA GGTTCGCTTG CACCAGCGGC TTCAGCTGTG TGTTTTTCAA 
CAGGATGTTC AACAGGTATT GGATTGGATT GAAAACCATG GAGAAGCCTT TCTTAGCAAA 
CATACTGGAG TGGGAAAATC CCTGCATCGT GCTAGAGCAT TACAGAAACG CCATGAAGAC 
TTTGAAGAAG TTGCACAGAA CACATACACC AACGCAGATA AACTTCTAGA AGCAGCCGAA 
CAGCTTGCTC AGACCGGAGA GTGTGATCCA GAGGAAATAT ATCAAGCTGC CCATCAGCTC 
GAAGACAGGA TACAGGATTT TGTCAGACGC GTGGAGCAGC GCAAGATCCT GCTGGACATG 
TCCGTTTCCT TTCACACACA CGTCAAGGAG CTGTGGACAT GGCTTGAAGA GTTGCAGAAA 
GAACTGCTTG ATGATGTGTA TGCAGAGTCT GTGGAGGCCG TCCAAGACCT GATTAAACGT 
TTTGGTCAGC AGCAGCAGAC CACTCTGCAG GTTACAGTCA ATGTCATAAA AGAAGGCGAA 
GATCTCATAC AGCAACTAAG GGATTCGGCC ATTTCTAGTA ACAAGACTCC CCATAACAGC 
TCGATCAACC ACATAGAGAC AGTCCTGCAG CAGCTGGATG AGGCCCAATC TCAGATGGAG 
GAGCTGTTTC AGGAACGCAA GATCAAGCTT GAGCTGTTTC TGCAGCTCCG CATATTTGAA 
AGAGATGCAA TAGATATAAT TTCAGACCTC GAGTCTTGGA ATGATGAGCT CTCTCAACAG 
ATGAATGACT TTGACACTGA AGATCTTACA ATTGCAGAAC AAAGGCTCCA GCATCATGCA 
GACAAAGCCC TGACCATGAA TAATTTGACC TTTGATGTCA TCCACCAAGG ACAGGATCTG 
CTGCAATATG TCAACGAAGT GCAGGCATCT GGTGTTGAGC TACTTTGTGA CAGGGATGTA 
GACATGGCAA CTCGTGTTCA GGATCTGCTG GAGTTTCTCC ATGAGAAGCA GCAGGAGCTG 
GATGTAGCAG CCGAGCAGCA CCGCAAGCAC TTGGAGCAGT GCGTGCAGCT GCGCCATCTG 
CAGGCGGAGG TCAAGCAGGT CTTGGGCTGG ATCCGCAATG GGGAGTCAAT GTTAAACGCG 
GGCCTTATCA CTGCTAGCTC TTTGCAAGAG GCAGAACAGT TACAGAGGGA ACATGAGCAG 
TTTCAACATG CAATAGAGAA AACACATCAA AGTGCCCTTC AGGTTCAGCA GAAAGCAGAG 
GCAATGCTAC AGGCTAATCA CTATGACATG GATATGATCA GGGAGTGTGC AGAGAAGGTT 
GCTTCCCATT GGCAACAACT GATGCTCAAG ATGGAGGACC GTCTCAAGCT TGTGAATGCC 
TCTGTTGCCT TCTATAAAAC TTCTGAACAG GTGTGCAGTG TATTGGAAAG CCTGGAGCAA 
GAGTATAAGC GGGAAGAAGA CTGGTGTGGG GGAGCAGACA AGCTGGGTCC CAACTCTGAA 
ACAGATCACG TTACCCCCAT GATAAGCAAA CACTTGGAAC AGAAGGAGGC ATTCCTAAAG 
GCTTGCACGC TGGCCCGGAG GAATGCTGAT GTCTTCCTGA AATACCTGCA CAGGAATAGC 
GTAAACATGC CAGGGATGGT GACGCACATC AAAGCACCTG AACAGCAAGT CAAAAATATC 
TTGAATGAAC TCTTCCAGCG GGAGAACCGT GTGCTGCATT ATTGGACCAT GCGTAAAAGA 
CGTCTTGATC AATGCCAACA GTACGTGGTG TTTGAAAGAA GTGCCAAACA GGCTCTGGAA 
TGGATTCATG ACAATGGGGA GTTTTATTTA TCCACACATA CTTCCACTGG TTCCAGTATC 
CAGCATACTC AAGAGCTGTT AAAAGAACAT GAAGAATTTC AGATCACAGC AAAGCAAACC 
AAAGAGAGGG TGAAGTTGTT GATACAGCTG GCCGATGGCT TTTGTGAAAA AGGGCATGCT 
CATGCAACAG AGATTAAAAA ATGTGTCACG GCAGTGGATA AAAGGTACAG AGACTTTTCC 
CTGCGCATGG AGAAATACAG GACCTCTTTA GAGAAAGCTC TGGGTATTTC CTCTGATTCC 
AATAAATCGA GCAAAAGCTT GCAGCTGGAT ATAATCCCAG CCAGTGTCCC TGGGTCAGAA 
GTGAAACTGC GTGATGCTGC TCATGAGCTT AATGAAGAAA AACGAAAATC TGCCCGCAGG 
AAAGAGTTTA TTATGGCTGA GCTAATTCAG ACAGAAAAGG CTTACGTAAG AGACCTCCGA 
GAATGCATGG ATACATATCT ATGGGAAATG ACAAGTGGAG TTGAGGAGAT CCCACCTGGT 
ATTGTAAACA AAGAGCATAT TATCTTTGGA AACATGCAGG AAATCTATGA GTTCCACAAT 
AATATATTCC TCAAGGAGCT AGAAAAATAT GAACAGTTAC CAGAAGATGT TGGACACTGC 
TTTGTGACAT GGGCAGACAA GTTCCAGATG TATGTTACTT ACTGCAAAAA TAAGCCAGAT 
TCTACCCAGC TAATATTAGA ACATGCAGGA GCATACTTTG ATGAGATACA ACAGCGACAT 
GGACTGGCCA ACTCTATCTC TTCTTACCTT ATCAAACCTG TTCAGAGGAT AACAAAATAT 
CAACTTCTTT TAAAGGAGTT GCTCACATGC TGTGAAGAAG GTAAAGGTGA GATAAAAGAT 
GGCCTGGAGG TGATGCTTAG TGTGCCAAAG CGAGCCAATG ATGCCATGCA CCTCAGTATG 
TTGGAAGGCT TTGATGAAAA TATAGAATCT CAGGGAGAGC TCATCCTTCA AGAATCCTTC 
CAAGTGTGGG ACCCCAAAAC TCTAATTCGA AAGGGAAGAG AGAGGCACCT TTTCCTTTTT 
GAGATGTCCT TGGTTTTCAG TAAAGAAGTA AAGGACTCAA GTGGAAGGAG CAAGTACATT 
TACAAGAGTA AACTGTTTAC TTCTGAATTG GGTGTCACAG AACACGTTGA AGGAGATCCC 
TGCAAGTTTG CTCTGTGGGT TGGAAGAACA CCAACTTCAG ACAACAAAAT TGTTCTCAAG 
GCATCCAGTA TAGAGAATAA ACAGGACTGG ATTAAACACA TCCGGGAAGT GATACAAGAA 
AGGACCATTC ATCTGAAAGG AGCATTGAAA GAGCCAATCC ACATCCCGAA AACAGCACCA 
ACAACAAAAC AGAAAGGAAG AAGAGATGGC GAGGACCTGG ACAGTCAAGG AGATGGTAGC 
AGCCAACCTG ATACTATTTC AATTGCCTCA CGAACTTCAC AGAACACACT TGACAGTGAT 
AAGTTGTCTG GTGGGTGCGA GCTGACGGTG GTAATCCATG ATTTCACCGC CTGCAACAGT 
AATGAGCTGA CAATCCGCCG TGGGCAGACT GTGGAGGTCC TGGAGCGACC CCATGACAAA 
CCCGACTGGT GTCTGGTTCG AACTACAGAT CGCTCCCCAG CTGCAGAAGG CCTGGTCCCC 
TGTGGGTCCC TCTGCATTGC TCACTCTCGC AGTAGTATGG AGATGGAGGG GATCTTTAAC 
CACAAAGACT CCCTTTCAGT CTCCAGCAAT GATGCCAGTC CTCCTGCTTC AGTAACATCT 
CTTCAACCCC ATATGATCGG GGCACAGAGC TCCCCGGGTC CCAAGCGCCC TGGCAACACT 
TTGAGAAAAT GGCTTACCAG TCCTGTGAGG CGTCTCAGCA GCGGGAAAGC AGATGGGCAC 
GTCAAAAAAC TGGCCCACAA GCACAAGAAG AGTAGAGAGG TTCGCAAAAG TGCTGATGCT 
GGTTCTCAGA AGGACTCTGA TGATAGTGCA GCAACCCCAC AAGATGAGAC TATTGAAGAG 
AGGGGTCGGA ACGAGGGGCT GAGCAGTGGC ACTCTCTCGA AGTCATCCTC TTCAGGCATG 
CAGAGCTGTG GAGAGGAAGA AGGTGAAGAG GGAGCAGATG CTGTACCACT TCCTCCTCCC 
ATGGCAATTC AGCAACACAG TCTTCTCCAG CCTGATTCAC AGGATGACAA GACCTCTTCT 
CGATTATTGG TGCGGCCTAC CAGCTCTGAG ACGCCCAGTG CTGCAGAACT AGTCAGTGCA 
ATTGAAGAGC TCGTCAAAAG CAAAATGGCC TTGGAAGACC GTCCAAGTTC CTTGTTAGTA 
GATCAAGGTG ACAGCAGCAG TCCATCCTTC AACCCTTCAG ATAACTCTCT TCTCTCCTCA 
TCATCACCTA TAGATGAGAT GGAAGAAAGG AAGTCCAGCT CCTTAAAAAG ACGACACTAT 
GTCTTGCAGG AATTAGTGGA GACAGAGCGT GATTACGTAC GAGATCTGGG CTATGTAGTT 
GAGGGTTACA TGGCACTTAT GAAGGAAGAT GGTGTTCCTG ATGATATGAA AGGCAAAGAC 
AAGATTGTAT TTGGAAATAT TCACCAGATT TATGACTGGC ACAGAGACTT CTTTTTAGGA 
GAGTTGGAGA AGTGCCTTGA AGATCCAGAA AAGCTGGGAT CCCTGTTTGT TAAGCATGAG 
AGAAGGTTGC ACATGTACAT AGTTTACTGC CAGAACAAAC CAAAGTCTGA GCACATTGTC 
TCAGAATACA TTGACACATT TTTTGAGGAT CTAAAGCAAC GTCTGGGCCA CAGACTTCAG 
CTCACAGACT TGTTAATAAA ACCTGTGCAG AGAATTATGA AATACCAGCT ATTACTAAAG 
GATTTCCTCA AATATTCTAA AAAAGCTAAC CTTGATACAA CAGAACTAGA GAGAGCTGTA 
GAGGTCATGT GTATAGTACC AAAACGGTGT AATGACATGA TGAATGTTGG CCGCCTGCAA 
GGATTTGATG GTAAAATTGT AGCCCAGGGT AAGCTGCTAC TCCAGGACAC ATTCTTGGTT 
ACGGACCAGG ACGCAGGTCT TCTGCCACGC TGCAAGGAGA GACGAGTCTT CCTGTTTGAG 
CAGATTGTTA TTTTCAGTGA GCCGCTAGAT AAAAAGAAAG GTTTCTCCAT GCCCGGATTC 
TTGTTTAAAA ACAGTATCAA GGTGAGTTGC CTTTCCCTGG AGGAAAATGT GGACAATGAT 
CCATGTAAAT TTGCACTAAC ATCAAGAACG GGTGATGTCA CGGAGACCTT TGTTTTGCAT 
TCTGCCAGTC CAGGAGTCCG CCAGCTGTGG ATCCATGAAA TTAACCAAAT TTTGGAAAAC 
CAGCGCAACT TTTTAAATGC CTTGACGTCT CCAATCGAGT ACCAGAGGAA CCACAGCAGT 
GGTGGAGGTG GCAGCAGCAG CAGCAGTGGT AACAGCAGCG GCCCCAGCAG CTGTAGCGGA 
ATCCCCAGCT CCAGCCGTAG CCGGCCATCC CGGATCCCCC AGCCTGTCAG ACACCATTCC 
CCAGTCCTGG TCTCCTCTGC AGCCTCGGCC CAAGCAGAGG CAGACAAGAT GTCAGGTATG 
TCCATTCACA ATCTCTCCCT GCCACACTAC AACACCTCCT TAGAAACTGG CCTGAGCCAG 
CCAGACAGGC ACCCTCCCAG TGCAGAACCG GACGGTCCTC AGAGAGAAGC TGAGCAGATT 
CCCAAGATGA AAGTTATTGA GAGTCCCAGG AAGACAGCGG GAAACATTTC TGGCTCAGCT 
GACGGAGCCC AGAAAGACTC ACGTGGAAGC TCAGAGGAAA GTCGCTCAAG AAACAGCATA 
GGATCACTGA CGCTGGGGAA GCCAAGGCCA GGAGCAATCT CACCAATGAA CTCTCCTCTC 
TCCACGACTT TCCCTTCTCC TTTTGGCAAA GAAACCTTTC CACCCAGCAG CCCCCTGCAG 
AAGGGCTCCT TTTGGAGCTC CATCCCAGCA TCCCCTGCCA GCCGCCCTGG CTCCTTCACT 
TTCCCTGGGG ACAATGACTC CCTCCAGCGG CAGGTCCACC GCCACTCTTC ACACAGCAAG 
GACACAGACC GCATGAGCAC ATGCTCCTCG ACCAGCGAGC AGTCAGTTCA CTCCACCCAG 
AGTAATGGGA GTGAAAGTAG CAGCAGTAGC AATATCTCCA CCATGCTGGT GACACACGAT 
TACACGGCAG TGAAGGAGGA TGAGATAAAT GTCTACCAAG GAGAGGTTGT GCAGATTCTA 
GCCAGCAACC AACAGAACAT GTTTTTGGTG TTCAGAGCCG CCACTGATCA GTGCCCCGCA 
GCTGAGGGCT GGATTCCCGG CTATGTCTTG GGGCATACCA GTGCAGTCAT CATGGACAAC 
CCTGATGGAA CCATCAAGAA GTCAACGTCT TGGCACACAG CACTCCGTTT AAGGAAGAAA 
TCTGAGAAAA AAGATAAAGA CGGCAAAAGG GAAGGCAAGC TAGAAAATGG TTACCGCAAA 
TCCCGAGAAG GACTTGCCAA CAAGGTTTCT GTAAAGCTTC TCAACCCCAA TTACATTTAT 
GATGTTCCCC CAGAGTTTAT AGTACCATTG AGTGAGGTAA CATGTGAGAC AGGAGAGACC 
ATCGTGCTTA GGTGTAAAGT CTGTGGTCGT CCCAAGGCTT CAGTTACTTG GAAAGGTCCT 
GATCATAACA CACTGAGCAA TGACGGTCAT CACAGCATTT CGTACAGTGA CTTAGGGGAG 
GCTTCACTGA AAATCATTGG CGTCACTGCA GAAGACGATG GTATCTATAC ATGTATAGCT 
GCAAACGATA TGGGTTCAGT TTCATCTTCC GCCAGTCTAC GAGTTTTAGG TCCTGGAAGT 
GATGGAATCA TGGTAATCTG GAAAGACAAC TTTGATTCCC TTTACAGTGA AGTGGCTGAG 
CTTGGCAGGG GCAGATTTTC TGTCGTTAAG AAATGTGACC AGAAGGGAAC CAAGCGAGCA 
GTAGCCACTA AGTTTGTGAA TAAGAAGTTG ATGAAGCGAG ACCAGGTTAC CCATGAACTT 
GGAGTCATGC AGAATCTCCA GCATCCCCAG CTCATTGGCC TTATTGATAC CTTTGAGACC 
GCCACCAACT ACATACTAGT ATTAGAAATG GCTGACCAGG GTCGCCTTCT AGACTACGTT 
GTGCGATGGG GAAACCTCAC TGAAGGGAAG ATCAGACTCT ACCTGGGAGA GATTCTGGAA 
GCTGTGCAGT ATCTTCACAA CTGCAGAATA GCACATTTGG ACCTAAAGCC TGAAAATATT 
TTGGTGGACC AGAGTTCCTC TAAGCCAACA ATAAAACTGG CTGACTTTGG AGATGCAGTC 
CAGCTCAATA CCACATATTA TATCCACCAG TTACTTGGAA ACCCAGAGTT TGCAGCTCCT 
GAAATTATTC TTGGAAATCC TGTTTCCCTC ACTTCAGATG TTTGGAGCAT TGGAGTGCTC 
ACGTATGTCC TTCTTAGTGG CGTCTCTCCT TTCCTGGATG AAAGCGTAGA GGAAACTTGC 
CTGAATATTT GCCGCTTAGA CTTTAGTTTC CCAGATGACT ACTTCAAAGG AGTTAGCCAG 
AAGGCCAAAG ATTTTGTATG TTTCCTGCTT CAGGATGACC CAGCTAAGCG TCCCTCAGCT 
GCACTGACCC TCCAGGAGCA ATGGCTGCAG CTGGGGAATA GCAAAGGCGC TGACAGCATT 
GACATATCCA GACTGACTTC GTTCATTGAG CGGCGAAAAC ACCAGAATGA TGTTCGACCT 
ATCCGTAGCA TTAAAAACTT CTTACAGAGC AGGCTCTTGC CAAGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TRIO cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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