bdnf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	bdnf
Entrez Gene ID	100037853
Full Name	brain-derived neurotrophic factor
Synonyms	bdnf-a,bdnf-b
General protein information	Preferred Names brain-derived neurotrophic factor Names brain-derived neurotrophic factor
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012960378.1	XP_012815832.1	brain-derived neurotrophic factor isoform X1
XM_031899709.1	XP_031755569.1	brain-derived neurotrophic factor isoform X2
XM_012960378.2	XP_012815832.1	brain-derived neurotrophic factor isoform X2
XM_031899708.1	XP_031755568.1	brain-derived neurotrophic factor isoform X1
NM_001097298.1	NP_001090767.1	brain-derived neurotrophic factor precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BDNF	NP_001026787.1
Canis lupus familiaris (dog)	BDNF	NP_001002975.1
Bos taurus (cattle)	BDNF	NP_001040072.1
Homo sapiens (human)	BDNF	NP_001137282.1
Pan troglodytes (chimpanzee)	BDNF	NP_001012443.1
Mus musculus (house mouse)	Bdnf	NP_031566.4
Danio rerio (zebrafish)	bdnf	NP_571670.2
Rattus norvegicus (Norway rat)	Bdnf	NP_036645.2
Xenopus tropicalis (tropical clawed frog)	bdnf	NP_001090767.1
Macaca mulatta (Rhesus monkey)	BDNF	XP_001089568.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterisation of a new regulator of BDNF signalling, Sprouty3, involved in axonal morphogenesis in vivo.
Panagiotaki N, Dajas-Bailador F, Amaya E, Papalopulu N, Dorey K
Development (Cambridge, England)137(23)4005-15(2010 Dec)

BDNF promotes target innervation of Xenopus mandibular trigeminal axons in vivo.
Huang JK, Dorey K, Ishibashi S, Amaya E
BMC developmental biology759(2007 May)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bdnf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bdnf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa02770	NM_001097298.1 Latest version!	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa02770	XM_031899709.1 Latest version!	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa02770	XM_012960378.2 Latest version!	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa29959	XM_031899708.1 Latest version!	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OXa02770	XM_012960378.1	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bdnf ( NM_001097298.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090767.1 Xenopus tropicalis brain-derived neurotrophic factor (bdnf), mRNA.

Price & Availability↑

CloneID	OXa02770
Clone ID Related Accession (Same CDS sequence)	XM_012960378.1 , XM_031899709.1 , XM_012960378.2 , NM_001097298.1
Accession Version	NM_001097298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1477497600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brain-derived neurotrophic factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF433430.1. ##Evidence-Data-START## Transcript exon combination :: EF433430.1 [ECO:0000332] ##Evidence-Data-END##

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCA GTTGCATGAA AGCTGCCCCC 
ATGAAAGAAG CCAGTGTCAG AGGACAAAGT GGCCTGGCCT ATCCGGGTCT TCGGACCCAT 
GGTACTCTTG AGAGCATAGG TGGTCTCGGT GTCTCAAGAG GAGGAGGACT TCCTTCACTG 
ACAGATACTT TTGAGCATGT CATAGAAGAA CTCATGGAAG AGGAACAAAG CATACGACAG 
AGCGAGGAAA GCAAGGACTC TGACTTGTAT TCATCTAGAG TTATGCTAAG CAGTCAAGTA 
CCTTTGGAGC CACCACTGCT TTTCCTACTT GAGGAGTACA AAAACTACTT GGATGCTGCA 
AACATGTCCA TGAGGGTCCG GCGCCACTCG GACCCAGCCA GGCGCGGAGA GCTGAGCGTG 
TGTGACAGTA TTAGCGAATG GGTTACAGCA GCAAACAAGA AAACTGCAGT GGACATGTCG 
GGGCAAACGG TTACTGTCCT CGAAAAAGTC CCCGTATCCA AAGGCCAACT GAAGCAATAT 
TTCTACGAGA CCAAATGTAA CCCTATGGGT TACATGAAAG AAGGCTGCAG AGGCATAGAC 
AAAAGGTACT GGAACTCTCA GTGCCGAACT ACTCAGTCTT ACGTGCGGGC TTTCACCATG 
GATAGCAAAA AAAAAGTTGG TTGGCGCTTT ATAAGAATAG ACACTTCTTG TGTATGTACA 
CTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090767.1
CDS	30..773
Translation	MTILFLTMVISYFSCMKAAPMKEASVRGQSGLAYPGLRTHGTLESIGGLGVSRGGGLPSLTDTFEHVIEELMEEEQSIRQSEESKDSDLYSSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAANKKTAVDMSGQTVTVLEKVPVSKGQLKQYFYETKCNPMGYMKEGCRGIDKRYWNSQCRTTQSYVRAFTMDSKKKVGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	NM_001097298.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097298.1

ORF Insert Sequence:

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCA GTTGCATGAA AGCTGCCCCC 
ATGAAAGAAG CCAGTGTCAG AGGACAAAGT GGCCTGGCCT ATCCGGGTCT TCGGACCCAT 
GGTACTCTTG AGAGCATAGG TGGTCTCGGT GTCTCAAGAG GAGGAGGACT TCCTTCACTG 
ACAGATACTT TTGAGCATGT CATAGAAGAA CTCATGGAAG AGGAACAAAG CATACGACAG 
AGCGAGGAAA GCAAGGACTC TGACTTGTAT TCATCTAGAG TTATGCTAAG CAGTCAAGTA 
CCTTTGGAGC CACCACTGCT TTTCCTACTT GAGGAGTACA AAAACTACTT GGATGCTGCA 
AACATGTCCA TGAGGGTCCG GCGCCACTCG GACCCAGCCA GGCGCGGAGA GCTGAGCGTG 
TGTGACAGTA TTAGCGAATG GGTTACAGCA GCAAACAAGA AAACTGCAGT GGACATGTCG 
GGGCAAACGG TTACTGTCCT CGAAAAAGTC CCCGTATCCA AAGGCCAACT GAAGCAATAT 
TTCTACGAGA CCAAATGTAA CCCTATGGGT TACATGAAAG AAGGCTGCAG AGGCATAGAC 
AAAAGGTACT GGAACTCTCA GTGCCGAACT ACTCAGTCTT ACGTGCGGGC TTTCACCATG 
GATAGCAAAA AAAAAGTTGG TTGGCGCTTT ATAAGAATAG ACACTTCTTG TGTATGTACA 
CTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bdnf ( XM_012960378.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815832.1 Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa02770
Clone ID Related Accession (Same CDS sequence)	XM_012960378.1 , XM_031899709.1 , XM_012960378.2 , NM_001097298.1
Accession Version	XM_012960378.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brain-derived neurotrophic factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCA GTTGCATGAA AGCTGCCCCC 
ATGAAAGAAG CCAGTGTCAG AGGACAAAGT GGCCTGGCCT ATCCGGGTCT TCGGACCCAT 
GGTACTCTTG AGAGCATAGG TGGTCTCGGT GTCTCAAGAG GAGGAGGACT TCCTTCACTG 
ACAGATACTT TTGAGCATGT CATAGAAGAA CTCATGGAAG AGGAACAAAG CATACGACAG 
AGCGAGGAAA GCAAGGACTC TGACTTGTAT TCATCTAGAG TTATGCTAAG CAGTCAAGTA 
CCTTTGGAGC CACCACTGCT TTTCCTACTT GAGGAGTACA AAAACTACTT GGATGCTGCA 
AACATGTCCA TGAGGGTCCG GCGCCACTCG GACCCAGCCA GGCGCGGAGA GCTGAGCGTG 
TGTGACAGTA TTAGCGAATG GGTTACAGCA GCAAACAAGA AAACTGCAGT GGACATGTCG 
GGGCAAACGG TTACTGTCCT CGAAAAAGTC CCCGTATCCA AAGGCCAACT GAAGCAATAT 
TTCTACGAGA CCAAATGTAA CCCTATGGGT TACATGAAAG AAGGCTGCAG AGGCATAGAC 
AAAAGGTACT GGAACTCTCA GTGCCGAACT ACTCAGTCTT ACGTGCGGGC TTTCACCATG 
GATAGCAAAA AAAAAGTTGG TTGGCGCTTT ATAAGAATAG ACACTTCTTG TGTATGTACA 
CTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815832.1
CDS	421..1164
Translation	MTILFLTMVISYFSCMKAAPMKEASVRGQSGLAYPGLRTHGTLESIGGLGVSRGGGLPSLTDTFEHVIEELMEEEQSIRQSEESKDSDLYSSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAANKKTAVDMSGQTVTVLEKVPVSKGQLKQYFYETKCNPMGYMKEGCRGIDKRYWNSQCRTTQSYVRAFTMDSKKKVGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_012960378.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960378.1

ORF Insert Sequence:

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCA GTTGCATGAA AGCTGCCCCC 
ATGAAAGAAG CCAGTGTCAG AGGACAAAGT GGCCTGGCCT ATCCGGGTCT TCGGACCCAT 
GGTACTCTTG AGAGCATAGG TGGTCTCGGT GTCTCAAGAG GAGGAGGACT TCCTTCACTG 
ACAGATACTT TTGAGCATGT CATAGAAGAA CTCATGGAAG AGGAACAAAG CATACGACAG 
AGCGAGGAAA GCAAGGACTC TGACTTGTAT TCATCTAGAG TTATGCTAAG CAGTCAAGTA 
CCTTTGGAGC CACCACTGCT TTTCCTACTT GAGGAGTACA AAAACTACTT GGATGCTGCA 
AACATGTCCA TGAGGGTCCG GCGCCACTCG GACCCAGCCA GGCGCGGAGA GCTGAGCGTG 
TGTGACAGTA TTAGCGAATG GGTTACAGCA GCAAACAAGA AAACTGCAGT GGACATGTCG 
GGGCAAACGG TTACTGTCCT CGAAAAAGTC CCCGTATCCA AAGGCCAACT GAAGCAATAT 
TTCTACGAGA CCAAATGTAA CCCTATGGGT TACATGAAAG AAGGCTGCAG AGGCATAGAC 
AAAAGGTACT GGAACTCTCA GTGCCGAACT ACTCAGTCTT ACGTGCGGGC TTTCACCATG 
GATAGCAAAA AAAAAGTTGG TTGGCGCTTT ATAAGAATAG ACACTTCTTG TGTATGTACA 
CTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bdnf ( XM_031899709.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755569.1 Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa02770
Clone ID Related Accession (Same CDS sequence)	XM_012960378.1 , XM_031899709.1 , XM_012960378.2 , NM_001097298.1
Accession Version	XM_031899709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brain-derived neurotrophic factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCA GTTGCATGAA AGCTGCCCCC 
ATGAAAGAAG CCAGTGTCAG AGGACAAAGT GGCCTGGCCT ATCCGGGTCT TCGGACCCAT 
GGTACTCTTG AGAGCATAGG TGGTCTCGGT GTCTCAAGAG GAGGAGGACT TCCTTCACTG 
ACAGATACTT TTGAGCATGT CATAGAAGAA CTCATGGAAG AGGAACAAAG CATACGACAG 
AGCGAGGAAA GCAAGGACTC TGACTTGTAT TCATCTAGAG TTATGCTAAG CAGTCAAGTA 
CCTTTGGAGC CACCACTGCT TTTCCTACTT GAGGAGTACA AAAACTACTT GGATGCTGCA 
AACATGTCCA TGAGGGTCCG GCGCCACTCG GACCCAGCCA GGCGCGGAGA GCTGAGCGTG 
TGTGACAGTA TTAGCGAATG GGTTACAGCA GCAAACAAGA AAACTGCAGT GGACATGTCG 
GGGCAAACGG TTACTGTCCT CGAAAAAGTC CCCGTATCCA AAGGCCAACT GAAGCAATAT 
TTCTACGAGA CCAAATGTAA CCCTATGGGT TACATGAAAG AAGGCTGCAG AGGCATAGAC 
AAAAGGTACT GGAACTCTCA GTGCCGAACT ACTCAGTCTT ACGTGCGGGC TTTCACCATG 
GATAGCAAAA AAAAAGTTGG TTGGCGCTTT ATAAGAATAG ACACTTCTTG TGTATGTACA 
CTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755569.1
CDS	398..1141
Translation	MTILFLTMVISYFSCMKAAPMKEASVRGQSGLAYPGLRTHGTLESIGGLGVSRGGGLPSLTDTFEHVIEELMEEEQSIRQSEESKDSDLYSSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAANKKTAVDMSGQTVTVLEKVPVSKGQLKQYFYETKCNPMGYMKEGCRGIDKRYWNSQCRTTQSYVRAFTMDSKKKVGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_031899709.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899709.1

ORF Insert Sequence:

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCA GTTGCATGAA AGCTGCCCCC 
ATGAAAGAAG CCAGTGTCAG AGGACAAAGT GGCCTGGCCT ATCCGGGTCT TCGGACCCAT 
GGTACTCTTG AGAGCATAGG TGGTCTCGGT GTCTCAAGAG GAGGAGGACT TCCTTCACTG 
ACAGATACTT TTGAGCATGT CATAGAAGAA CTCATGGAAG AGGAACAAAG CATACGACAG 
AGCGAGGAAA GCAAGGACTC TGACTTGTAT TCATCTAGAG TTATGCTAAG CAGTCAAGTA 
CCTTTGGAGC CACCACTGCT TTTCCTACTT GAGGAGTACA AAAACTACTT GGATGCTGCA 
AACATGTCCA TGAGGGTCCG GCGCCACTCG GACCCAGCCA GGCGCGGAGA GCTGAGCGTG 
TGTGACAGTA TTAGCGAATG GGTTACAGCA GCAAACAAGA AAACTGCAGT GGACATGTCG 
GGGCAAACGG TTACTGTCCT CGAAAAAGTC CCCGTATCCA AAGGCCAACT GAAGCAATAT 
TTCTACGAGA CCAAATGTAA CCCTATGGGT TACATGAAAG AAGGCTGCAG AGGCATAGAC 
AAAAGGTACT GGAACTCTCA GTGCCGAACT ACTCAGTCTT ACGTGCGGGC TTTCACCATG 
GATAGCAAAA AAAAAGTTGG TTGGCGCTTT ATAAGAATAG ACACTTCTTG TGTATGTACA 
CTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bdnf ( XM_012960378.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815832.1 Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa02770
Clone ID Related Accession (Same CDS sequence)	XM_012960378.1 , XM_031899709.1 , XM_012960378.2 , NM_001097298.1
Accession Version	XM_012960378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brain-derived neurotrophic factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCA GTTGCATGAA AGCTGCCCCC 
ATGAAAGAAG CCAGTGTCAG AGGACAAAGT GGCCTGGCCT ATCCGGGTCT TCGGACCCAT 
GGTACTCTTG AGAGCATAGG TGGTCTCGGT GTCTCAAGAG GAGGAGGACT TCCTTCACTG 
ACAGATACTT TTGAGCATGT CATAGAAGAA CTCATGGAAG AGGAACAAAG CATACGACAG 
AGCGAGGAAA GCAAGGACTC TGACTTGTAT TCATCTAGAG TTATGCTAAG CAGTCAAGTA 
CCTTTGGAGC CACCACTGCT TTTCCTACTT GAGGAGTACA AAAACTACTT GGATGCTGCA 
AACATGTCCA TGAGGGTCCG GCGCCACTCG GACCCAGCCA GGCGCGGAGA GCTGAGCGTG 
TGTGACAGTA TTAGCGAATG GGTTACAGCA GCAAACAAGA AAACTGCAGT GGACATGTCG 
GGGCAAACGG TTACTGTCCT CGAAAAAGTC CCCGTATCCA AAGGCCAACT GAAGCAATAT 
TTCTACGAGA CCAAATGTAA CCCTATGGGT TACATGAAAG AAGGCTGCAG AGGCATAGAC 
AAAAGGTACT GGAACTCTCA GTGCCGAACT ACTCAGTCTT ACGTGCGGGC TTTCACCATG 
GATAGCAAAA AAAAAGTTGG TTGGCGCTTT ATAAGAATAG ACACTTCTTG TGTATGTACA 
CTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815832.1
CDS	430..1173
Translation	MTILFLTMVISYFSCMKAAPMKEASVRGQSGLAYPGLRTHGTLESIGGLGVSRGGGLPSLTDTFEHVIEELMEEEQSIRQSEESKDSDLYSSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAANKKTAVDMSGQTVTVLEKVPVSKGQLKQYFYETKCNPMGYMKEGCRGIDKRYWNSQCRTTQSYVRAFTMDSKKKVGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_012960378.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960378.2

ORF Insert Sequence:

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCA GTTGCATGAA AGCTGCCCCC 
ATGAAAGAAG CCAGTGTCAG AGGACAAAGT GGCCTGGCCT ATCCGGGTCT TCGGACCCAT 
GGTACTCTTG AGAGCATAGG TGGTCTCGGT GTCTCAAGAG GAGGAGGACT TCCTTCACTG 
ACAGATACTT TTGAGCATGT CATAGAAGAA CTCATGGAAG AGGAACAAAG CATACGACAG 
AGCGAGGAAA GCAAGGACTC TGACTTGTAT TCATCTAGAG TTATGCTAAG CAGTCAAGTA 
CCTTTGGAGC CACCACTGCT TTTCCTACTT GAGGAGTACA AAAACTACTT GGATGCTGCA 
AACATGTCCA TGAGGGTCCG GCGCCACTCG GACCCAGCCA GGCGCGGAGA GCTGAGCGTG 
TGTGACAGTA TTAGCGAATG GGTTACAGCA GCAAACAAGA AAACTGCAGT GGACATGTCG 
GGGCAAACGG TTACTGTCCT CGAAAAAGTC CCCGTATCCA AAGGCCAACT GAAGCAATAT 
TTCTACGAGA CCAAATGTAA CCCTATGGGT TACATGAAAG AAGGCTGCAG AGGCATAGAC 
AAAAGGTACT GGAACTCTCA GTGCCGAACT ACTCAGTCTT ACGTGCGGGC TTTCACCATG 
GATAGCAAAA AAAAAGTTGG TTGGCGCTTT ATAAGAATAG ACACTTCTTG TGTATGTACA 
CTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bdnf ( XM_031899708.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755568.1 Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29959
Clone ID Related Accession (Same CDS sequence)	XM_031899708.1
Accession Version	XM_031899708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brain-derived neurotrophic factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATC AGGTGAGAAG AGTGATGACC ATCCTTTTCC TTACTATGGT TATTTCATAC 
TTCAGTTGCA TGAAAGCTGC CCCCATGAAA GAAGCCAGTG TCAGAGGACA AAGTGGCCTG 
GCCTATCCGG GTCTTCGGAC CCATGGTACT CTTGAGAGCA TAGGTGGTCT CGGTGTCTCA 
AGAGGAGGAG GACTTCCTTC ACTGACAGAT ACTTTTGAGC ATGTCATAGA AGAACTCATG 
GAAGAGGAAC AAAGCATACG ACAGAGCGAG GAAAGCAAGG ACTCTGACTT GTATTCATCT 
AGAGTTATGC TAAGCAGTCA AGTACCTTTG GAGCCACCAC TGCTTTTCCT ACTTGAGGAG 
TACAAAAACT ACTTGGATGC TGCAAACATG TCCATGAGGG TCCGGCGCCA CTCGGACCCA 
GCCAGGCGCG GAGAGCTGAG CGTGTGTGAC AGTATTAGCG AATGGGTTAC AGCAGCAAAC 
AAGAAAACTG CAGTGGACAT GTCGGGGCAA ACGGTTACTG TCCTCGAAAA AGTCCCCGTA 
TCCAAAGGCC AACTGAAGCA ATATTTCTAC GAGACCAAAT GTAACCCTAT GGGTTACATG 
AAAGAAGGCT GCAGAGGCAT AGACAAAAGG TACTGGAACT CTCAGTGCCG AACTACTCAG 
TCTTACGTGC GGGCTTTCAC CATGGATAGC AAAAAAAAAG TTGGTTGGCG CTTTATAAGA 
ATAGACACTT CTTGTGTATG TACACTGACC ATTAAAAGGG GAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755568.1
CDS	225..992
Translation	MFHQVRRVMTILFLTMVISYFSCMKAAPMKEASVRGQSGLAYPGLRTHGTLESIGGLGVSRGGGLPSLTDTFEHVIEELMEEEQSIRQSEESKDSDLYSSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAANKKTAVDMSGQTVTVLEKVPVSKGQLKQYFYETKCNPMGYMKEGCRGIDKRYWNSQCRTTQSYVRAFTMDSKKKVGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_031899708.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899708.1

ORF Insert Sequence:

ATGTTCCATC AGGTGAGAAG AGTGATGACC ATCCTTTTCC TTACTATGGT TATTTCATAC 
TTCAGTTGCA TGAAAGCTGC CCCCATGAAA GAAGCCAGTG TCAGAGGACA AAGTGGCCTG 
GCCTATCCGG GTCTTCGGAC CCATGGTACT CTTGAGAGCA TAGGTGGTCT CGGTGTCTCA 
AGAGGAGGAG GACTTCCTTC ACTGACAGAT ACTTTTGAGC ATGTCATAGA AGAACTCATG 
GAAGAGGAAC AAAGCATACG ACAGAGCGAG GAAAGCAAGG ACTCTGACTT GTATTCATCT 
AGAGTTATGC TAAGCAGTCA AGTACCTTTG GAGCCACCAC TGCTTTTCCT ACTTGAGGAG 
TACAAAAACT ACTTGGATGC TGCAAACATG TCCATGAGGG TCCGGCGCCA CTCGGACCCA 
GCCAGGCGCG GAGAGCTGAG CGTGTGTGAC AGTATTAGCG AATGGGTTAC AGCAGCAAAC 
AAGAAAACTG CAGTGGACAT GTCGGGGCAA ACGGTTACTG TCCTCGAAAA AGTCCCCGTA 
TCCAAAGGCC AACTGAAGCA ATATTTCTAC GAGACCAAAT GTAACCCTAT GGGTTACATG 
AAAGAAGGCT GCAGAGGCAT AGACAAAAGG TACTGGAACT CTCAGTGCCG AACTACTCAG 
TCTTACGTGC GGGCTTTCAC CATGGATAGC AAAAAAAAAG TTGGTTGGCG CTTTATAAGA 
ATAGACACTT CTTGTGTATG TACACTGACC ATTAAAAGGG GAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterisation of a new regulator of BDNF signalling, Sprouty3, involved in axonal morphogenesis in vivo.
Development (Cambridge, England)137(23)4005-15(2010 Dec)
Panagiotaki N,Dajas-Bailador F,Amaya E,Papalopulu N,Dorey K

BDNF promotes target innervation of Xenopus mandibular trigeminal axons in vivo.
BMC developmental biology759(2007 May)
Huang JK,Dorey K,Ishibashi S,Amaya E

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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bdnf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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