bdnf cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bdnf
Entrez Gene ID	58118
Full Name	brain-derived neurotrophic factor
General protein information	Preferred Names brain-derived neurotrophic factor Names brain-derived neurotrophic factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308648.1	NP_001295577.1	brain-derived neurotrophic factor isoform 1
NM_001308649.1	NP_001295578.1	brain-derived neurotrophic factor isoform 2
XM_009303234.2	XP_009301509.1	brain-derived neurotrophic factor isoform X1
NM_131595.2	NP_571670.2	brain-derived neurotrophic factor isoform 2
XM_009303234.3	XP_009301509.1	brain-derived neurotrophic factor isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BDNF	NP_001026787.1
Canis lupus familiaris (dog)	BDNF	NP_001002975.1
Bos taurus (cattle)	BDNF	NP_001040072.1
Homo sapiens (human)	BDNF	NP_001137282.1
Pan troglodytes (chimpanzee)	BDNF	NP_001012443.1
Mus musculus (house mouse)	Bdnf	NP_031566.4
Danio rerio (zebrafish)	bdnf	NP_571670.2
Rattus norvegicus (Norway rat)	Bdnf	NP_036645.2
Xenopus tropicalis (tropical clawed frog)	bdnf	NP_001090767.1
Macaca mulatta (Rhesus monkey)	BDNF	XP_001089568.2

Gene Ontology
Bibliography

Related articles in PubMed

Dynactin1 depletion leads to neuromuscular synapse instability and functional abnormalities.
Bercier V, Hubbard JM, Fidelin K, Duroure K, Auer TO, Revenu C, Wyart C, Del Bene F
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Effect of newer anti-epileptic drugs (AEDs) on the cognitive status in pentylenetetrazol induced seizures in a zebrafish model.
Choo BKM, Kundap UP, Johan Arief MFB, Kumari Y, Yap JL, Wong CP, Othman I, Shaikh MF
Progress in neuro-psychopharmacology & biological psychiatry92483-493(2019 06)

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F
Cell reports27(5)1567-1578.e5(2019 04)

Subtle morphometric, behavioral and gene expression effects in larval zebrafish exposed to PFHxA, PFHxS and 6:2 FTOH.
Annunziato KM, Jantzen CE, Gronske MC, Cooper KR
Aquatic toxicology (Amsterdam, Netherlands)208126-137(2019 Mar)

Neurotrophin receptor Ntrk2b function in the maintenance of dopamine and serotonin neurons in zebrafish.
Sahu MP, Pazos-Boubeta Y, Pajanoja C, Rozov S, Panula P, Castrén E
Scientific reports9(1)2036(2019 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bdnf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bdnf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00791	NM_131595.2 Latest version!	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa00791	NM_001308649.1 Latest version!	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa13096	NM_001308648.1 Latest version!	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa00791	XM_009303234.3 Latest version!	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
ODa00791	XM_009303234.2	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bdnf ( NM_001308649.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001295578.1 Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ODa00791
Clone ID Related Accession (Same CDS sequence)	NM_001308649.1 , XM_009303234.2 , NM_131595.2 , XM_009303234.3
Accession Version	NM_001308649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	brain-derived neurotrophic factor isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU459028.7 and AL935207.6. On May 15, 2015 this sequence version replaced XM_005168910.2. Transcript Variant: This variant (3) uses an alternate exon in the 5' UTR which results in the use of a downstream AUG compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CK140832.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505372, SAMEA3505373 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCATCC TGTTCGTTAC TATGGTTATT TCATACTTCA GTTGCATGAG AGCTGCGCCC 
ATGAGAGAGA TCCCGGGCGT GCAGGGGGGC CACCGGGCCG AGGGCTACCT GGGCGCCGCT 
GCCGCTGCTG CCGCTGCCGT CACATCGGGC AGCCGAGGCC ACGGGACTCC ACAGAGTGGG 
GGCGGGCTGC CCTCGCTCAC GGACACTTTC GAGCAGGTCA TTGAGGAGTT GCTTGAGGTG 
GAAGGGGAAG CGACGCAGCA ACTGGGGCCT GGGGCCGACC AGGGCCAAGG AGGGGGCGGC 
CCTATAGATG CGGCAGACTC GAAGGACGTT GACCTGTATG CCTCGCGGGT GATGATCAGC 
AACCAAGTGC CTTTGGAGCC GCCGTTACTC TTTCTCTTGG AGGAATACAA AAACTACCTG 
GATGCCGCCA ACATGTCGAT GCGTGTGCGG CGACACTCGG ACCCCGCACG GCGGGGGGAG 
CTCAGCGTTT GTGACAGTAT TAGCCAGTGG GTGACAGCTG TGGACAAAAA GACGGCAATA 
GACATGTCGG GCCAGACGGT CACCGTTCTG GAGAAGGTCC CCGTGACTAA TGGTCAGCTG 
AAGCAATACT TTTACGAGAC CAAATGCAAC CCCTTGGGGT ACACAAAGGA GGGCTGCCGA 
GGAATAGACA AGCGGCACTA TAACTCGCAA TGCCGGACAA CCCAGTCTTA CGTGCGAGCC 
CTTACCATGG ATAGCAAAAG GAAGATCGGC TGGCGGTTTA TACGGATAGA CACTTCGTGT 
GTATGCACAT TGACCATTAA GAGGGGCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295578.1
CDS	778..1590
Translation	MTILFVTMVISYFSCMRAAPMREIPGVQGGHRAEGYLGAAAAAAAAVTSGSRGHGTPQSGGGLPSLTDTFEQVIEELLEVEGEATQQLGPGADQGQGGGGPIDAADSKDVDLYASRVMISNQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISQWVTAVDKKTAIDMSGQTVTVLEKVPVTNGQLKQYFYETKCNPLGYTKEGCRGIDKRHYNSQCRTTQSYVRALTMDSKRKIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	NM_001308649.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308649.1

ORF Insert Sequence:

ATGACCATCC TGTTCGTTAC TATGGTTATT TCATACTTCA GTTGCATGAG AGCTGCGCCC 
ATGAGAGAGA TCCCGGGCGT GCAGGGGGGC CACCGGGCCG AGGGCTACCT GGGCGCCGCT 
GCCGCTGCTG CCGCTGCCGT CACATCGGGC AGCCGAGGCC ACGGGACTCC ACAGAGTGGG 
GGCGGGCTGC CCTCGCTCAC GGACACTTTC GAGCAGGTCA TTGAGGAGTT GCTTGAGGTG 
GAAGGGGAAG CGACGCAGCA ACTGGGGCCT GGGGCCGACC AGGGCCAAGG AGGGGGCGGC 
CCTATAGATG CGGCAGACTC GAAGGACGTT GACCTGTATG CCTCGCGGGT GATGATCAGC 
AACCAAGTGC CTTTGGAGCC GCCGTTACTC TTTCTCTTGG AGGAATACAA AAACTACCTG 
GATGCCGCCA ACATGTCGAT GCGTGTGCGG CGACACTCGG ACCCCGCACG GCGGGGGGAG 
CTCAGCGTTT GTGACAGTAT TAGCCAGTGG GTGACAGCTG TGGACAAAAA GACGGCAATA 
GACATGTCGG GCCAGACGGT CACCGTTCTG GAGAAGGTCC CCGTGACTAA TGGTCAGCTG 
AAGCAATACT TTTACGAGAC CAAATGCAAC CCCTTGGGGT ACACAAAGGA GGGCTGCCGA 
GGAATAGACA AGCGGCACTA TAACTCGCAA TGCCGGACAA CCCAGTCTTA CGTGCGAGCC 
CTTACCATGG ATAGCAAAAG GAAGATCGGC TGGCGGTTTA TACGGATAGA CACTTCGTGT 
GTATGCACAT TGACCATTAA GAGGGGCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bdnf ( NM_131595.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571670.2 Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa00791
Clone ID Related Accession (Same CDS sequence)	NM_001308649.1 , XM_009303234.2 , NM_131595.2 , XM_009303234.3
Accession Version	NM_131595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	brain-derived neurotrophic factor isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC058301.1. On Jan 25, 2004 this sequence version replaced NM_131595.1. Transcript Variant: This variant uses an alternate 5' exon which results in the use of a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058301.1, BC066399.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168448 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCATCC TGTTCGTTAC TATGGTTATT TCATACTTCA GTTGCATGAG AGCTGCGCCC 
ATGAGAGAGA TCCCGGGCGT GCAGGGGGGC CACCGGGCCG AGGGCTACCT GGGCGCCGCT 
GCCGCTGCTG CCGCTGCCGT CACATCGGGC AGCCGAGGCC ACGGGACTCC ACAGAGTGGG 
GGCGGGCTGC CCTCGCTCAC GGACACTTTC GAGCAGGTCA TTGAGGAGTT GCTTGAGGTG 
GAAGGGGAAG CGACGCAGCA ACTGGGGCCT GGGGCCGACC AGGGCCAAGG AGGGGGCGGC 
CCTATAGATG CGGCAGACTC GAAGGACGTT GACCTGTATG CCTCGCGGGT GATGATCAGC 
AACCAAGTGC CTTTGGAGCC GCCGTTACTC TTTCTCTTGG AGGAATACAA AAACTACCTG 
GATGCCGCCA ACATGTCGAT GCGTGTGCGG CGACACTCGG ACCCCGCACG GCGGGGGGAG 
CTCAGCGTTT GTGACAGTAT TAGCCAGTGG GTGACAGCTG TGGACAAAAA GACGGCAATA 
GACATGTCGG GCCAGACGGT CACCGTTCTG GAGAAGGTCC CCGTGACTAA TGGTCAGCTG 
AAGCAATACT TTTACGAGAC CAAATGCAAC CCCTTGGGGT ACACAAAGGA GGGCTGCCGA 
GGAATAGACA AGCGGCACTA TAACTCGCAA TGCCGGACAA CCCAGTCTTA CGTGCGAGCC 
CTTACCATGG ATAGCAAAAG GAAGATCGGC TGGCGGTTTA TACGGATAGA CACTTCGTGT 
GTATGCACAT TGACCATTAA GAGGGGCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571670.2
CDS	305..1117
Translation	MTILFVTMVISYFSCMRAAPMREIPGVQGGHRAEGYLGAAAAAAAAVTSGSRGHGTPQSGGGLPSLTDTFEQVIEELLEVEGEATQQLGPGADQGQGGGGPIDAADSKDVDLYASRVMISNQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISQWVTAVDKKTAIDMSGQTVTVLEKVPVTNGQLKQYFYETKCNPLGYTKEGCRGIDKRHYNSQCRTTQSYVRALTMDSKRKIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	NM_131595.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131595.2

ORF Insert Sequence:

ATGACCATCC TGTTCGTTAC TATGGTTATT TCATACTTCA GTTGCATGAG AGCTGCGCCC 
ATGAGAGAGA TCCCGGGCGT GCAGGGGGGC CACCGGGCCG AGGGCTACCT GGGCGCCGCT 
GCCGCTGCTG CCGCTGCCGT CACATCGGGC AGCCGAGGCC ACGGGACTCC ACAGAGTGGG 
GGCGGGCTGC CCTCGCTCAC GGACACTTTC GAGCAGGTCA TTGAGGAGTT GCTTGAGGTG 
GAAGGGGAAG CGACGCAGCA ACTGGGGCCT GGGGCCGACC AGGGCCAAGG AGGGGGCGGC 
CCTATAGATG CGGCAGACTC GAAGGACGTT GACCTGTATG CCTCGCGGGT GATGATCAGC 
AACCAAGTGC CTTTGGAGCC GCCGTTACTC TTTCTCTTGG AGGAATACAA AAACTACCTG 
GATGCCGCCA ACATGTCGAT GCGTGTGCGG CGACACTCGG ACCCCGCACG GCGGGGGGAG 
CTCAGCGTTT GTGACAGTAT TAGCCAGTGG GTGACAGCTG TGGACAAAAA GACGGCAATA 
GACATGTCGG GCCAGACGGT CACCGTTCTG GAGAAGGTCC CCGTGACTAA TGGTCAGCTG 
AAGCAATACT TTTACGAGAC CAAATGCAAC CCCTTGGGGT ACACAAAGGA GGGCTGCCGA 
GGAATAGACA AGCGGCACTA TAACTCGCAA TGCCGGACAA CCCAGTCTTA CGTGCGAGCC 
CTTACCATGG ATAGCAAAAG GAAGATCGGC TGGCGGTTTA TACGGATAGA CACTTCGTGT 
GTATGCACAT TGACCATTAA GAGGGGCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bdnf ( NM_001308648.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001295577.1 Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa13096
Clone ID Related Accession (Same CDS sequence)	NM_001308648.1
Accession Version	NM_001308648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	brain-derived neurotrophic factor isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU459028.7 and AL935207.6. On May 15, 2015 this sequence version replaced XM_005168909.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EB947034.1, CO933510.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCAAC AGGTTAGAAG AGTGATGACC ATCCTGTTCG TTACTATGGT TATTTCATAC 
TTCAGTTGCA TGAGAGCTGC GCCCATGAGA GAGATCCCGG GCGTGCAGGG GGGCCACCGG 
GCCGAGGGCT ACCTGGGCGC CGCTGCCGCT GCTGCCGCTG CCGTCACATC GGGCAGCCGA 
GGCCACGGGA CTCCACAGAG TGGGGGCGGG CTGCCCTCGC TCACGGACAC TTTCGAGCAG 
GTCATTGAGG AGTTGCTTGA GGTGGAAGGG GAAGCGACGC AGCAACTGGG GCCTGGGGCC 
GACCAGGGCC AAGGAGGGGG CGGCCCTATA GATGCGGCAG ACTCGAAGGA CGTTGACCTG 
TATGCCTCGC GGGTGATGAT CAGCAACCAA GTGCCTTTGG AGCCGCCGTT ACTCTTTCTC 
TTGGAGGAAT ACAAAAACTA CCTGGATGCC GCCAACATGT CGATGCGTGT GCGGCGACAC 
TCGGACCCCG CACGGCGGGG GGAGCTCAGC GTTTGTGACA GTATTAGCCA GTGGGTGACA 
GCTGTGGACA AAAAGACGGC AATAGACATG TCGGGCCAGA CGGTCACCGT TCTGGAGAAG 
GTCCCCGTGA CTAATGGTCA GCTGAAGCAA TACTTTTACG AGACCAAATG CAACCCCTTG 
GGGTACACAA AGGAGGGCTG CCGAGGAATA GACAAGCGGC ACTATAACTC GCAATGCCGG 
ACAACCCAGT CTTACGTGCG AGCCCTTACC ATGGATAGCA AAAGGAAGAT CGGCTGGCGG 
TTTATACGGA TAGACACTTC GTGTGTATGC ACATTGACCA TTAAGAGGGG CAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295577.1
CDS	384..1220
Translation	MFQQVRRVMTILFVTMVISYFSCMRAAPMREIPGVQGGHRAEGYLGAAAAAAAAVTSGSRGHGTPQSGGGLPSLTDTFEQVIEELLEVEGEATQQLGPGADQGQGGGGPIDAADSKDVDLYASRVMISNQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISQWVTAVDKKTAIDMSGQTVTVLEKVPVTNGQLKQYFYETKCNPLGYTKEGCRGIDKRHYNSQCRTTQSYVRALTMDSKRKIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	NM_001308648.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308648.1

ORF Insert Sequence:

ATGTTCCAAC AGGTTAGAAG AGTGATGACC ATCCTGTTCG TTACTATGGT TATTTCATAC 
TTCAGTTGCA TGAGAGCTGC GCCCATGAGA GAGATCCCGG GCGTGCAGGG GGGCCACCGG 
GCCGAGGGCT ACCTGGGCGC CGCTGCCGCT GCTGCCGCTG CCGTCACATC GGGCAGCCGA 
GGCCACGGGA CTCCACAGAG TGGGGGCGGG CTGCCCTCGC TCACGGACAC TTTCGAGCAG 
GTCATTGAGG AGTTGCTTGA GGTGGAAGGG GAAGCGACGC AGCAACTGGG GCCTGGGGCC 
GACCAGGGCC AAGGAGGGGG CGGCCCTATA GATGCGGCAG ACTCGAAGGA CGTTGACCTG 
TATGCCTCGC GGGTGATGAT CAGCAACCAA GTGCCTTTGG AGCCGCCGTT ACTCTTTCTC 
TTGGAGGAAT ACAAAAACTA CCTGGATGCC GCCAACATGT CGATGCGTGT GCGGCGACAC 
TCGGACCCCG CACGGCGGGG GGAGCTCAGC GTTTGTGACA GTATTAGCCA GTGGGTGACA 
GCTGTGGACA AAAAGACGGC AATAGACATG TCGGGCCAGA CGGTCACCGT TCTGGAGAAG 
GTCCCCGTGA CTAATGGTCA GCTGAAGCAA TACTTTTACG AGACCAAATG CAACCCCTTG 
GGGTACACAA AGGAGGGCTG CCGAGGAATA GACAAGCGGC ACTATAACTC GCAATGCCGG 
ACAACCCAGT CTTACGTGCG AGCCCTTACC ATGGATAGCA AAAGGAAGAT CGGCTGGCGG 
TTTATACGGA TAGACACTTC GTGTGTATGC ACATTGACCA TTAAGAGGGG CAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bdnf ( XM_009303234.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301509.1 Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00791
Clone ID Related Accession (Same CDS sequence)	NM_001308649.1 , XM_009303234.2 , NM_131595.2 , XM_009303234.3
Accession Version	XM_009303234.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	brain-derived neurotrophic factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TGTTCGTTAC TATGGTTATT TCATACTTCA GTTGCATGAG AGCTGCGCCC 
ATGAGAGAGA TCCCGGGCGT GCAGGGGGGC CACCGGGCCG AGGGCTACCT GGGCGCCGCT 
GCCGCTGCTG CCGCTGCCGT CACATCGGGC AGCCGAGGCC ACGGGACTCC ACAGAGTGGG 
GGCGGGCTGC CCTCGCTCAC GGACACTTTC GAGCAGGTCA TTGAGGAGTT GCTTGAGGTG 
GAAGGGGAAG CGACGCAGCA ACTGGGGCCT GGGGCCGACC AGGGCCAAGG AGGGGGCGGC 
CCTATAGATG CGGCAGACTC GAAGGACGTT GACCTGTATG CCTCGCGGGT GATGATCAGC 
AACCAAGTGC CTTTGGAGCC GCCGTTACTC TTTCTCTTGG AGGAATACAA AAACTACCTG 
GATGCCGCCA ACATGTCGAT GCGTGTGCGG CGACACTCGG ACCCCGCACG GCGGGGGGAG 
CTCAGCGTTT GTGACAGTAT TAGCCAGTGG GTGACAGCTG TGGACAAAAA GACGGCAATA 
GACATGTCGG GCCAGACGGT CACCGTTCTG GAGAAGGTCC CCGTGACTAA TGGTCAGCTG 
AAGCAATACT TTTACGAGAC CAAATGCAAC CCCTTGGGGT ACACAAAGGA GGGCTGCCGA 
GGAATAGACA AGCGGCACTA TAACTCGCAA TGCCGGACAA CCCAGTCTTA CGTGCGAGCC 
CTTACCATGG ATAGCAAAAG GAAGATCGGC TGGCGGTTTA TACGGATAGA CACTTCGTGT 
GTATGCACAT TGACCATTAA GAGGGGCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301509.1
CDS	108..920
Translation	MTILFVTMVISYFSCMRAAPMREIPGVQGGHRAEGYLGAAAAAAAAVTSGSRGHGTPQSGGGLPSLTDTFEQVIEELLEVEGEATQQLGPGADQGQGGGGPIDAADSKDVDLYASRVMISNQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISQWVTAVDKKTAIDMSGQTVTVLEKVPVTNGQLKQYFYETKCNPLGYTKEGCRGIDKRHYNSQCRTTQSYVRALTMDSKRKIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_009303234.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303234.2

ORF Insert Sequence:

ATGACCATCC TGTTCGTTAC TATGGTTATT TCATACTTCA GTTGCATGAG AGCTGCGCCC 
ATGAGAGAGA TCCCGGGCGT GCAGGGGGGC CACCGGGCCG AGGGCTACCT GGGCGCCGCT 
GCCGCTGCTG CCGCTGCCGT CACATCGGGC AGCCGAGGCC ACGGGACTCC ACAGAGTGGG 
GGCGGGCTGC CCTCGCTCAC GGACACTTTC GAGCAGGTCA TTGAGGAGTT GCTTGAGGTG 
GAAGGGGAAG CGACGCAGCA ACTGGGGCCT GGGGCCGACC AGGGCCAAGG AGGGGGCGGC 
CCTATAGATG CGGCAGACTC GAAGGACGTT GACCTGTATG CCTCGCGGGT GATGATCAGC 
AACCAAGTGC CTTTGGAGCC GCCGTTACTC TTTCTCTTGG AGGAATACAA AAACTACCTG 
GATGCCGCCA ACATGTCGAT GCGTGTGCGG CGACACTCGG ACCCCGCACG GCGGGGGGAG 
CTCAGCGTTT GTGACAGTAT TAGCCAGTGG GTGACAGCTG TGGACAAAAA GACGGCAATA 
GACATGTCGG GCCAGACGGT CACCGTTCTG GAGAAGGTCC CCGTGACTAA TGGTCAGCTG 
AAGCAATACT TTTACGAGAC CAAATGCAAC CCCTTGGGGT ACACAAAGGA GGGCTGCCGA 
GGAATAGACA AGCGGCACTA TAACTCGCAA TGCCGGACAA CCCAGTCTTA CGTGCGAGCC 
CTTACCATGG ATAGCAAAAG GAAGATCGGC TGGCGGTTTA TACGGATAGA CACTTCGTGT 
GTATGCACAT TGACCATTAA GAGGGGCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bdnf ( XM_009303234.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301509.1 Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.

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CloneID	ODa00791
Clone ID Related Accession (Same CDS sequence)	NM_001308649.1 , XM_009303234.2 , NM_131595.2 , XM_009303234.3
Accession Version	XM_009303234.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	brain-derived neurotrophic factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TGTTCGTTAC TATGGTTATT TCATACTTCA GTTGCATGAG AGCTGCGCCC 
ATGAGAGAGA TCCCGGGCGT GCAGGGGGGC CACCGGGCCG AGGGCTACCT GGGCGCCGCT 
GCCGCTGCTG CCGCTGCCGT CACATCGGGC AGCCGAGGCC ACGGGACTCC ACAGAGTGGG 
GGCGGGCTGC CCTCGCTCAC GGACACTTTC GAGCAGGTCA TTGAGGAGTT GCTTGAGGTG 
GAAGGGGAAG CGACGCAGCA ACTGGGGCCT GGGGCCGACC AGGGCCAAGG AGGGGGCGGC 
CCTATAGATG CGGCAGACTC GAAGGACGTT GACCTGTATG CCTCGCGGGT GATGATCAGC 
AACCAAGTGC CTTTGGAGCC GCCGTTACTC TTTCTCTTGG AGGAATACAA AAACTACCTG 
GATGCCGCCA ACATGTCGAT GCGTGTGCGG CGACACTCGG ACCCCGCACG GCGGGGGGAG 
CTCAGCGTTT GTGACAGTAT TAGCCAGTGG GTGACAGCTG TGGACAAAAA GACGGCAATA 
GACATGTCGG GCCAGACGGT CACCGTTCTG GAGAAGGTCC CCGTGACTAA TGGTCAGCTG 
AAGCAATACT TTTACGAGAC CAAATGCAAC CCCTTGGGGT ACACAAAGGA GGGCTGCCGA 
GGAATAGACA AGCGGCACTA TAACTCGCAA TGCCGGACAA CCCAGTCTTA CGTGCGAGCC 
CTTACCATGG ATAGCAAAAG GAAGATCGGC TGGCGGTTTA TACGGATAGA CACTTCGTGT 
GTATGCACAT TGACCATTAA GAGGGGCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301509.1
CDS	2884..3696
Translation	MTILFVTMVISYFSCMRAAPMREIPGVQGGHRAEGYLGAAAAAAAAVTSGSRGHGTPQSGGGLPSLTDTFEQVIEELLEVEGEATQQLGPGADQGQGGGGPIDAADSKDVDLYASRVMISNQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISQWVTAVDKKTAIDMSGQTVTVLEKVPVTNGQLKQYFYETKCNPLGYTKEGCRGIDKRHYNSQCRTTQSYVRALTMDSKRKIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_009303234.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio brain-derived neurotrophic factor (bdnf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303234.3

ORF Insert Sequence:

ATGACCATCC TGTTCGTTAC TATGGTTATT TCATACTTCA GTTGCATGAG AGCTGCGCCC 
ATGAGAGAGA TCCCGGGCGT GCAGGGGGGC CACCGGGCCG AGGGCTACCT GGGCGCCGCT 
GCCGCTGCTG CCGCTGCCGT CACATCGGGC AGCCGAGGCC ACGGGACTCC ACAGAGTGGG 
GGCGGGCTGC CCTCGCTCAC GGACACTTTC GAGCAGGTCA TTGAGGAGTT GCTTGAGGTG 
GAAGGGGAAG CGACGCAGCA ACTGGGGCCT GGGGCCGACC AGGGCCAAGG AGGGGGCGGC 
CCTATAGATG CGGCAGACTC GAAGGACGTT GACCTGTATG CCTCGCGGGT GATGATCAGC 
AACCAAGTGC CTTTGGAGCC GCCGTTACTC TTTCTCTTGG AGGAATACAA AAACTACCTG 
GATGCCGCCA ACATGTCGAT GCGTGTGCGG CGACACTCGG ACCCCGCACG GCGGGGGGAG 
CTCAGCGTTT GTGACAGTAT TAGCCAGTGG GTGACAGCTG TGGACAAAAA GACGGCAATA 
GACATGTCGG GCCAGACGGT CACCGTTCTG GAGAAGGTCC CCGTGACTAA TGGTCAGCTG 
AAGCAATACT TTTACGAGAC CAAATGCAAC CCCTTGGGGT ACACAAAGGA GGGCTGCCGA 
GGAATAGACA AGCGGCACTA TAACTCGCAA TGCCGGACAA CCCAGTCTTA CGTGCGAGCC 
CTTACCATGG ATAGCAAAAG GAAGATCGGC TGGCGGTTTA TACGGATAGA CACTTCGTGT 
GTATGCACAT TGACCATTAA GAGGGGCAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynactin1 depletion leads to neuromuscular synapse instability and functional abnormalities.
Molecular neurodegeneration14(1)27(2019 07)
Bercier V,Hubbard JM,Fidelin K,Duroure K,Auer TO,Revenu C,Wyart C,Del Bene F

Effect of newer anti-epileptic drugs (AEDs) on the cognitive status in pentylenetetrazol induced seizures in a zebrafish model.
Progress in neuro-psychopharmacology & biological psychiatry92483-493(2019 06)
Choo BKM,Kundap UP,Johan Arief MFB,Kumari Y,Yap JL,Wong CP,Othman I,Shaikh MF

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Cell reports27(5)1567-1578.e5(2019 04)
Xue Y,Liu D,Cui G,Ding Y,Ai D,Gao S,Zhang Y,Suo S,Wang X,Lv P,Zhou C,Li Y,Chen X,Peng G,Jing N,Han JJ,Liu F

Subtle morphometric, behavioral and gene expression effects in larval zebrafish exposed to PFHxA, PFHxS and 6:2 FTOH.
Aquatic toxicology (Amsterdam, Netherlands)208126-137(2019 Mar)
Annunziato KM,Jantzen CE,Gronske MC,Cooper KR

Neurotrophin receptor Ntrk2b function in the maintenance of dopamine and serotonin neurons in zebrafish.
Scientific reports9(1)2036(2019 Feb)
Sahu MP,Pazos-Boubeta Y,Pajanoja C,Rozov S,Panula P,Castrén E

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bdnf cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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