Defb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Defb1
Entrez Gene ID	83687
Full Name	defensin beta 1
Synonyms	CDK4
General protein information	Preferred Names defensin beta 1 Names beta-defensin 1 BD-1 rBD-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5
Summary	cationic peptides with broad-spectrum antimicrobial activity; may mediate mucosal defenses of several organs [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031810.1	NP_113998.1	beta-defensin 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Defb1	NP_031869.1
Rattus norvegicus (Norway rat)	Defb1	NP_113998.1

Gene Ontology
Bibliography

Related articles in PubMed

Deficient human β-defensin 1 underlies male infertility associated with poor sperm motility and genital tract infection.
Diao R, Fok KL, Chen H, Yu MK, Duan Y, Chung CM, Li Z, Wu H, Li Z, Zhang H, Ji Z, Zhen W, Ng CF, Gui Y, Cai Z, Chan HC
Science translational medicine6(249)249ra108(2014 Aug)

Structural basis for the interaction of human β-defensin 6 and its putative chemokine receptor CCR2 and breast cancer microvesicles.
De Paula VS, Gomes NS, Lima LG, Miyamoto CA, Monteiro RQ, Almeida FC, Valente AP
Journal of molecular biology425(22)4479-95(2013 Nov)

Role of defensins in corneal epithelial barrier function against Pseudomonas aeruginosa traversal.
Augustin DK, Heimer SR, Tam C, Li WY, Le Due JM, Evans DJ, Fleiszig SM
Infection and immunity79(2)595-605(2011 Feb)

A polymorphism in the 5' UTR of the DEFB1 gene is associated with the lung phenotype in F508del homozygous Italian cystic fibrosis patients.
Crovella S, Segat L, Amato A, Athanasakis E, Bezzerri V, Braggion C, Casciaro R, Castaldo G, Colombo C, Covone AE, De Rose V, Gagliardini R, Lanzara C, Minicucci L, Morgutti M, Nicolis E, Pardo F, Quattrucci S, Raia V, Ravazzolo R, Seia M, Stanzial V, Termini L, Zazzeron L, Cabrini G, Gasparini P
Clinical chemistry and laboratory medicine49(1)49-54(2011 Jan)

Analysis of DEFB1 regulatory SNPs in cystic fibrosis patients from North-Eastern Italy.
Segat L, Morgutti M, Athanasakis E, Trevisiol C, Amaddeo A, Poli F, Crovella S
International journal of immunogenetics37(3)169-75(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Defb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Defb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08787	NM_031810.1 Latest version!	Rattus norvegicus defensin beta 1 (Defb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Defb1 ( NM_031810.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113998.1 Rattus norvegicus defensin beta 1 (Defb1), mRNA.

Price & Availability↑

CloneID	ORa08787
Clone ID Related Accession (Same CDS sequence)	NM_031810.1
Accession Version	NM_031810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Rattus norvegicus(Norway rat)
Product	beta-defensin 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF093536.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF093536.1, FM127743.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689595, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGAAAACTC ATTACTTTCT CCTGGTGATG TTATTTTTTC TCTTCTCCCA GATGGAGCTG 
GGTGCTGGCA TTCTCACAAG TCTTGGACGC AGAACAGATC AATACCGATG CCTCCAAAAT 
GGAGGATTCT GTCTCCGCTC CAGCTGCCCA TCTCATACCA AACTACAAGG AACATGTAAA 
CCAGATAAGC CCAACTGTTG CAGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113998.1
CDS	67..276
Translation	MKTHYFLLVMLFFLFSQMELGAGILTSLGRRTDQYRCLQNGGFCLRSSCPSHTKLQGTCKPDKPNCCRS

Target ORF information:

RefSeq Version	NM_031810.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus defensin beta 1 (Defb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031810.1

ORF Insert Sequence:

ATGAAAACTC ATTACTTTCT CCTGGTGATG TTATTTTTTC TCTTCTCCCA GATGGAGCTG 
GGTGCTGGCA TTCTCACAAG TCTTGGACGC AGAACAGATC AATACCGATG CCTCCAAAAT 
GGAGGATTCT GTCTCCGCTC CAGCTGCCCA TCTCATACCA AACTACAAGG AACATGTAAA 
CCAGATAAGC CCAACTGTTG CAGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficient human β-defensin 1 underlies male infertility associated with poor sperm motility and genital tract infection.
Science translational medicine6(249)249ra108(2014 Aug)
Diao R,Fok KL,Chen H,Yu MK,Duan Y,Chung CM,Li Z,Wu H,Li Z,Zhang H,Ji Z,Zhen W,Ng CF,Gui Y,Cai Z,Chan HC

Structural basis for the interaction of human β-defensin 6 and its putative chemokine receptor CCR2 and breast cancer microvesicles.
Journal of molecular biology425(22)4479-95(2013 Nov)
De Paula VS,Gomes NS,Lima LG,Miyamoto CA,Monteiro RQ,Almeida FC,Valente AP

Role of defensins in corneal epithelial barrier function against Pseudomonas aeruginosa traversal.
Infection and immunity79(2)595-605(2011 Feb)
Augustin DK,Heimer SR,Tam C,Li WY,Le Due JM,Evans DJ,Fleiszig SM

A polymorphism in the 5' UTR of the DEFB1 gene is associated with the lung phenotype in F508del homozygous Italian cystic fibrosis patients.
Clinical chemistry and laboratory medicine49(1)49-54(2011 Jan)
Crovella S,Segat L,Amato A,Athanasakis E,Bezzerri V,Braggion C,Casciaro R,Castaldo G,Colombo C,Covone AE,De Rose V,Gagliardini R,Lanzara C,Minicucci L,Morgutti M,Nicolis E,Pardo F,Quattrucci S,Raia V,Ravazzolo R,Seia M,Stanzial V,Termini L,Zazzeron L,Cabrini G,Gasparini P

Analysis of DEFB1 regulatory SNPs in cystic fibrosis patients from North-Eastern Italy.
International journal of immunogenetics37(3)169-75(2010 Jun)
Segat L,Morgutti M,Athanasakis E,Trevisiol C,Amaddeo A,Poli F,Crovella S

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Defb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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