Agl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agl
Entrez Gene ID	362029
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
General protein information	Preferred Names amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Names glycogen debranching enzyme amylo-1, 6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q41

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233229.3	XP_006233291.1	glycogen debranching enzyme isoform X1
NM_001108564.1	NP_001102034.1	glycogen debranching enzyme

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Homo sapiens (human)	AGL	NP_000635.2
Macaca mulatta (Rhesus monkey)	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Gene Ontology
Bibliography

Related articles in PubMed

A role for AGL ubiquitination in the glycogen storage disorders of Lafora and Cori's disease.
Cheng A, Zhang M, Gentry MS, Worby CA, Dixon JE, Saltiel AR
Genes & development21(19)2399-409(2007 Oct)

Hepatic and neuromuscular forms of glycogenosis type III: nine mutations in AGL.
Lucchiari S, Pagliarani S, Salani S, Filocamo M, Di Rocco M, Melis D, Rodolico C, Musumeci O, Toscano A, Bresolin N, Comi GP
Human mutation27(6)600-1(2006 Jun)

Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum.
Lees SJ, Chen YT, Williams JH
Acta physiologica Scandinavica181(2)239-45(2004 Jun)

The genetic association database.
Becker KG, Barnes KC, Bright TJ, Wang SA
Nature genetics36(5)431-2(2004 May)

Inverse relationship of skeletal muscle glycogen from wild-type and genetically modified mice to their phosphorylase a activity.
Schliselfeld LH, Danon MJ
Biochemical and biophysical research communications290(2)874-7(2002 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Agl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07805	NM_001108564.1 Latest version!	Rattus norvegicus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa07805	XM_006233229.3 Latest version!	Rattus norvegicus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agl ( NM_001108564.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102034.1 Rattus norvegicus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), mRNA.

Price & Availability↑

CloneID	ORa07805
Clone ID Related Accession (Same CDS sequence)	XM_006233229.3 , NM_001108564.1
Accession Version	NM_001108564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	glycogen debranching enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473952.1. On or before Oct 4, 2007 this sequence version replaced XM_342331.3, XM_001073642.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTATTGAACG AAATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAGCA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TTACTGTTTA TACAAATTAC CCATTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCTTGGATTG GGAAAACCCA ACAGAGAGAG AAGATGATTC GGACAAATAC 
TGTAAAGTTC ATCTGCAGCA GTCTGGGTCT TTTCAGTACT ACTTCCTTCA AGGACAGGAG 
AAAAGTGGCG GAGGTTACAT AGTTGTGGAT CCTATTCTGC GAGTTGGAGC TGATAATCAC 
GTGCTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CAAAGTGTTT AGGACCCTTT 
GATGAGTGGG AAAGCCGACT ACGGGTGGCA AAGGAGTCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGGCTCTCGG ACTGTCCAGG TCATGCTATT CCCTTGCCGA TCAGTTAGAA 
TTAAATCCTG ACTTCTCAAG GCCCAATAGA AGATACACCT GGGATGATGT TGGGCAGCTA 
GTAGAGAAGC TGAAGAGGGA GTGGAACATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCTGCTA ATAGTAAGTG GATCCATGAG CATCCAGAAT CTGCGTACAA TCTTGTAAAT 
TCTCCACACT TGAAACCTGC CTGGGTCCTA GACAGAGCGC TCTGGCATTT GTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTC TAATTGAAAA TGACCATCAC 
ATGAACTGCA TTCGGAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA GCTCTGGGAG 
TTTTTCCAAG TAGATGTTCA GAAAGCGGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCGAAA GAACATCTTA AAATTATTCA GGACCCCGAA 
TTCAGGCGGC TGGGCTGTGC TGTAGATATG AACATTGCTC TGGCGACCTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAATTGGT TCCGTAAGAG ACTCGAGGAG 
TTAAATTCAG AGAAACACCA CCTCACCAGC TGTCATCAGG AACAGGCCGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGGCTGGCG GGCCATGGCC CTAAACTAGG ACCCGTCACC 
AGGAAGTACC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGAAGAAAT GGCCTTGTCT 
GCAGAAGAGT CCCTGCTCCA CCGCCCAGAT AAAGCTTGCT TTCTGATGGC ACACAATGGC 
TGGGTGATGG GCGATGACCC GCTTCGGAAC TTTGCTGAGC CAGGTTCCAA CGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTGAAATTGC GCTATGGGAA CAAACCGGAG 
GACTGCCCTT ACCTGTGGGC ACACATGAAG AAATACACTG AGATCACTGC CACTCACTTC 
CAGGGAGTGC GCCTTGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGATCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCAT ATAACAGTCA CGAAGAGGGC AGATTGGTCT ACCGGTACGG AGGGGAGCCC 
GTCGGATCCT TCGTTCAGCC CTGTTTGAGG CCTCTTATGC CAGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCAATTGTGC ACAGATCAGC CTATGATGCG 
CTTCCAAGTA CCACAGTTGT CTCCATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGGG CATCCCCATC GGATACAGGA GATGTTAGCA TCCACAGTGG GATTATTGCA 
GCCAGATGTG CCATCAACAG ACTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCAGTAACCA GGCACTCACC AAGCATCCAT 
CAGTCTGTTG TGGCTGTATC GAGAACGGCT TTTAGGAATC CCAAGACTTC ATTTTACAGC 
AAAGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAGA GGAATACAAA ACCTTATAGG AAAGATGAGA ATTCAATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACGTATC CAGCTTCATG AAAGTAAAAT TGTCAAACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAAAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGCCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCACCT GACCCAATTC AGCTCCCATT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGA 
GGTTGCTATG ATATACCAAA CTGGTCGTCC CTTAAGTATG CAGGTCTGCA AGGATTAATG 
TCCGTGCTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGAAAACTTG 
AGGTCTGGAG ACTGGATGAT CGACTACGTC AGTGGCCGGC TAATCTCACG ATCAGGGAGT 
ATTGCTGAAG TTGGAAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCGCGA 
TATCTCATCC CATGTTACTT TGATGCTATA TTAATCGGTG CATATACCAC TCTGCTGGAT 
GTAGCCTGGA AACAGATGTC AAGCTTTGTT CAGAATGGCT CGACCTTCGT GAAGCACCTG 
TCCCTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAGTGTC CTTGTCTGCC GCTGCTTTCA 
CCCTCACTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CGAAGGAGAA AGAGCAGTGC 
TGTGCTTCCT TGGCTGCAGG CTTACCTCAC TTTTCTTCTG GTCTTTTTCG CTGCTGGGGG 
AGGGATACTT TCATTGCACT TCGAGGGATG CTGCTGGTTA CTGGTCGCTA CCTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTTGTACC CTGAGGCATG GTCTCATTCC TAACCTCCTT 
GGCGAAGGAA CGTATGCCAG GTACAACTGT CGTGATGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAAGAT GGTTCCAAAT GGTCTAGACA TCCTCAAATG CCCAGTTTCC 
AGAATGTATC CAACAGATGA CTCTGCACCT TTGCCTGCTG GGACTCTGGA TCAGCCTTTG 
TTTGAAGTCA TACAGGAAGC TATGCAGAGG CACATGCAAG GCATTCAGTT CCGAGAGCGG 
AATGCCGGTC CACAGATCGA TCGAAACATG AAGGATGAGG GTTTCAACAT AACCGTGGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACATGGATG 
GATAAAATGG GAGAGAGTGA CAGAGCTAGA AACAGAGGGA TCCCAGCCAC CCCAAGAGAT 
GGGTCTGCTG TGGAAATCGT GGGCTTGTGT AAATCTGCTG TTCGTTGGTT GCTGGAATTG 
TCCAAGAAAA ACATTTTTCC TTATCATGAA GTCAGAGTAA GAAGACATGG AAAGGTTGTA 
GCAGTCTCCT ATGATGAGTG GAACAGAAAA ATACAAAACA GTTTTGAAAA GCTCTTTCAT 
GTTTCCGAAG ACCCTTCAGA TCCTAACGAG AAGCATCCGA ATCTGGTTCA CAAGCGGGGC 
ATCTACAAGG ACAGCTACGG TGCGTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCCAAC 
TTTACCATAG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CTGAAAAGGC TTGGAAGGCT 
TTGGAAATTG CAGAAGGAAA ATTGCTTGGT CCCCTTGGCA TGAAGACTTT GGATCCAGAT 
GACATGGTTT ACTGTGGAGT CTATGACAAT GCCTTAGACA ATGACAACTA CAACCTTGCC 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTATGGC CGATTGGGTA TTTTCTTCGT 
GCAAAGTTGT ATTTTTCCAA AATGATGGGC CCAGAGACTG CTGCGAAGAC TGTATTTTTG 
GTTAAAAATG TTCTTTCTAG ACATTATGTT CATCTTGAGA GATCCCCATG GAAAGGACTT 
CCAGAGCTCA CCAATGAGAA TGGCCAGTAC TGCCCTTTCA GCTGTGAGAC ACAGGCATGG 
TCGATGGCTG CTGTTCTCGA AACCCTCTAT GATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102034.1
CDS	544..5142
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGEAFNREKFRSLDWENPTEREDDSDKYCKVHLQQSGSFQYYFLQGQEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQALGLSRSCYSLADQLELNPDFSRPNRRYTWDDVGQLVEKLKREWNILCITDVVYNHTAANSKWIHEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVADGKYREKGVPALIENDHHMNCIRKIIWEDIFPRIQLWEFFQVDVQKAVEQFRRLLSQENRRVTKSEPKEHLKIIQDPEFRRLGCAVDMNIALATFIPHDNGPAAIEECCNWFRKRLEELNSEKHHLTSCHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFEEMALSAEESLLHRPDKACFLMAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATHFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPSDTGDVSIHSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGMPNMTVELRERIQLHESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNSDPILKIPFASIASKLTLAELNQVLYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFACTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITVGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVRRHGKVVAVSYDEWNRKIQNSFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEGKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMGPETAAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSMAAVLETLYDL

Target ORF information:

RefSeq Version	NM_001108564.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108564.1

ORF Insert Sequence:

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTATTGAACG AAATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAGCA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TTACTGTTTA TACAAATTAC CCATTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCTTGGATTG GGAAAACCCA ACAGAGAGAG AAGATGATTC GGACAAATAC 
TGTAAAGTTC ATCTGCAGCA GTCTGGGTCT TTTCAGTACT ACTTCCTTCA AGGACAGGAG 
AAAAGTGGCG GAGGTTACAT AGTTGTGGAT CCTATTCTGC GAGTTGGAGC TGATAATCAC 
GTGCTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CAAAGTGTTT AGGACCCTTT 
GATGAGTGGG AAAGCCGACT ACGGGTGGCA AAGGAGTCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGGCTCTCGG ACTGTCCAGG TCATGCTATT CCCTTGCCGA TCAGTTAGAA 
TTAAATCCTG ACTTCTCAAG GCCCAATAGA AGATACACCT GGGATGATGT TGGGCAGCTA 
GTAGAGAAGC TGAAGAGGGA GTGGAACATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCTGCTA ATAGTAAGTG GATCCATGAG CATCCAGAAT CTGCGTACAA TCTTGTAAAT 
TCTCCACACT TGAAACCTGC CTGGGTCCTA GACAGAGCGC TCTGGCATTT GTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTC TAATTGAAAA TGACCATCAC 
ATGAACTGCA TTCGGAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA GCTCTGGGAG 
TTTTTCCAAG TAGATGTTCA GAAAGCGGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCGAAA GAACATCTTA AAATTATTCA GGACCCCGAA 
TTCAGGCGGC TGGGCTGTGC TGTAGATATG AACATTGCTC TGGCGACCTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAATTGGT TCCGTAAGAG ACTCGAGGAG 
TTAAATTCAG AGAAACACCA CCTCACCAGC TGTCATCAGG AACAGGCCGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGGCTGGCG GGCCATGGCC CTAAACTAGG ACCCGTCACC 
AGGAAGTACC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGAAGAAAT GGCCTTGTCT 
GCAGAAGAGT CCCTGCTCCA CCGCCCAGAT AAAGCTTGCT TTCTGATGGC ACACAATGGC 
TGGGTGATGG GCGATGACCC GCTTCGGAAC TTTGCTGAGC CAGGTTCCAA CGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTGAAATTGC GCTATGGGAA CAAACCGGAG 
GACTGCCCTT ACCTGTGGGC ACACATGAAG AAATACACTG AGATCACTGC CACTCACTTC 
CAGGGAGTGC GCCTTGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGATCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCAT ATAACAGTCA CGAAGAGGGC AGATTGGTCT ACCGGTACGG AGGGGAGCCC 
GTCGGATCCT TCGTTCAGCC CTGTTTGAGG CCTCTTATGC CAGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCAATTGTGC ACAGATCAGC CTATGATGCG 
CTTCCAAGTA CCACAGTTGT CTCCATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGGG CATCCCCATC GGATACAGGA GATGTTAGCA TCCACAGTGG GATTATTGCA 
GCCAGATGTG CCATCAACAG ACTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCAGTAACCA GGCACTCACC AAGCATCCAT 
CAGTCTGTTG TGGCTGTATC GAGAACGGCT TTTAGGAATC CCAAGACTTC ATTTTACAGC 
AAAGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAGA GGAATACAAA ACCTTATAGG AAAGATGAGA ATTCAATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACGTATC CAGCTTCATG AAAGTAAAAT TGTCAAACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAAAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGCCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCACCT GACCCAATTC AGCTCCCATT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGA 
GGTTGCTATG ATATACCAAA CTGGTCGTCC CTTAAGTATG CAGGTCTGCA AGGATTAATG 
TCCGTGCTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGAAAACTTG 
AGGTCTGGAG ACTGGATGAT CGACTACGTC AGTGGCCGGC TAATCTCACG ATCAGGGAGT 
ATTGCTGAAG TTGGAAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCGCGA 
TATCTCATCC CATGTTACTT TGATGCTATA TTAATCGGTG CATATACCAC TCTGCTGGAT 
GTAGCCTGGA AACAGATGTC AAGCTTTGTT CAGAATGGCT CGACCTTCGT GAAGCACCTG 
TCCCTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAGTGTC CTTGTCTGCC GCTGCTTTCA 
CCCTCACTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CGAAGGAGAA AGAGCAGTGC 
TGTGCTTCCT TGGCTGCAGG CTTACCTCAC TTTTCTTCTG GTCTTTTTCG CTGCTGGGGG 
AGGGATACTT TCATTGCACT TCGAGGGATG CTGCTGGTTA CTGGTCGCTA CCTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTTGTACC CTGAGGCATG GTCTCATTCC TAACCTCCTT 
GGCGAAGGAA CGTATGCCAG GTACAACTGT CGTGATGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAAGAT GGTTCCAAAT GGTCTAGACA TCCTCAAATG CCCAGTTTCC 
AGAATGTATC CAACAGATGA CTCTGCACCT TTGCCTGCTG GGACTCTGGA TCAGCCTTTG 
TTTGAAGTCA TACAGGAAGC TATGCAGAGG CACATGCAAG GCATTCAGTT CCGAGAGCGG 
AATGCCGGTC CACAGATCGA TCGAAACATG AAGGATGAGG GTTTCAACAT AACCGTGGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACATGGATG 
GATAAAATGG GAGAGAGTGA CAGAGCTAGA AACAGAGGGA TCCCAGCCAC CCCAAGAGAT 
GGGTCTGCTG TGGAAATCGT GGGCTTGTGT AAATCTGCTG TTCGTTGGTT GCTGGAATTG 
TCCAAGAAAA ACATTTTTCC TTATCATGAA GTCAGAGTAA GAAGACATGG AAAGGTTGTA 
GCAGTCTCCT ATGATGAGTG GAACAGAAAA ATACAAAACA GTTTTGAAAA GCTCTTTCAT 
GTTTCCGAAG ACCCTTCAGA TCCTAACGAG AAGCATCCGA ATCTGGTTCA CAAGCGGGGC 
ATCTACAAGG ACAGCTACGG TGCGTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCCAAC 
TTTACCATAG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CTGAAAAGGC TTGGAAGGCT 
TTGGAAATTG CAGAAGGAAA ATTGCTTGGT CCCCTTGGCA TGAAGACTTT GGATCCAGAT 
GACATGGTTT ACTGTGGAGT CTATGACAAT GCCTTAGACA ATGACAACTA CAACCTTGCC 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTATGGC CGATTGGGTA TTTTCTTCGT 
GCAAAGTTGT ATTTTTCCAA AATGATGGGC CCAGAGACTG CTGCGAAGAC TGTATTTTTG 
GTTAAAAATG TTCTTTCTAG ACATTATGTT CATCTTGAGA GATCCCCATG GAAAGGACTT 
CCAGAGCTCA CCAATGAGAA TGGCCAGTAC TGCCCTTTCA GCTGTGAGAC ACAGGCATGG 
TCGATGGCTG CTGTTCTCGA AACCCTCTAT GATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( XM_006233229.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233291.1 Rattus norvegicus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07805
Clone ID Related Accession (Same CDS sequence)	XM_006233229.3 , NM_001108564.1
Accession Version	XM_006233229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTATTGAACG AAATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAGCA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TTACTGTTTA TACAAATTAC CCATTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCTTGGATTG GGAAAACCCA ACAGAGAGAG AAGATGATTC GGACAAATAC 
TGTAAAGTTC ATCTGCAGCA GTCTGGGTCT TTTCAGTACT ACTTCCTTCA AGGACAGGAG 
AAAAGTGGCG GAGGTTACAT AGTTGTGGAT CCTATTCTGC GAGTTGGAGC TGATAATCAC 
GTGCTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CAAAGTGTTT AGGACCCTTT 
GATGAGTGGG AAAGCCGACT ACGGGTGGCA AAGGAGTCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGGCTCTCGG ACTGTCCAGG TCATGCTATT CCCTTGCCGA TCAGTTAGAA 
TTAAATCCTG ACTTCTCAAG GCCCAATAGA AGATACACCT GGGATGATGT TGGGCAGCTA 
GTAGAGAAGC TGAAGAGGGA GTGGAACATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCTGCTA ATAGTAAGTG GATCCATGAG CATCCAGAAT CTGCGTACAA TCTTGTAAAT 
TCTCCACACT TGAAACCTGC CTGGGTCCTA GACAGAGCGC TCTGGCATTT GTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTC TAATTGAAAA TGACCATCAC 
ATGAACTGCA TTCGGAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA GCTCTGGGAG 
TTTTTCCAAG TAGATGTTCA GAAAGCGGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCGAAA GAACATCTTA AAATTATTCA GGACCCCGAA 
TTCAGGCGGC TGGGCTGTGC TGTAGATATG AACATTGCTC TGGCGACCTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAATTGGT TCCGTAAGAG ACTCGAGGAG 
TTAAATTCAG AGAAACACCA CCTCACCAGC TGTCATCAGG AACAGGCCGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGGCTGGCG GGCCATGGCC CTAAACTAGG ACCCGTCACC 
AGGAAGTACC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGAAGAAAT GGCCTTGTCT 
GCAGAAGAGT CCCTGCTCCA CCGCCCAGAT AAAGCTTGCT TTCTGATGGC ACACAATGGC 
TGGGTGATGG GCGATGACCC GCTTCGGAAC TTTGCTGAGC CAGGTTCCAA CGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTGAAATTGC GCTATGGGAA CAAACCGGAG 
GACTGCCCTT ACCTGTGGGC ACACATGAAG AAATACACTG AGATCACTGC CACTCACTTC 
CAGGGAGTGC GCCTTGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGATCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCAT ATAACAGTCA CGAAGAGGGC AGATTGGTCT ACCGGTACGG AGGGGAGCCC 
GTCGGATCCT TCGTTCAGCC CTGTTTGAGG CCTCTTATGC CAGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCAATTGTGC ACAGATCAGC CTATGATGCG 
CTTCCAAGTA CCACAGTTGT CTCCATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGGG CATCCCCATC GGATACAGGA GATGTTAGCA TCCACAGTGG GATTATTGCA 
GCCAGATGTG CCATCAACAG ACTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCAGTAACCA GGCACTCACC AAGCATCCAT 
CAGTCTGTTG TGGCTGTATC GAGAACGGCT TTTAGGAATC CCAAGACTTC ATTTTACAGC 
AAAGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAGA GGAATACAAA ACCTTATAGG AAAGATGAGA ATTCAATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACGTATC CAGCTTCATG AAAGTAAAAT TGTCAAACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAAAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGCCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCACCT GACCCAATTC AGCTCCCATT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGA 
GGTTGCTATG ATATACCAAA CTGGTCGTCC CTTAAGTATG CAGGTCTGCA AGGATTAATG 
TCCGTGCTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGAAAACTTG 
AGGTCTGGAG ACTGGATGAT CGACTACGTC AGTGGCCGGC TAATCTCACG ATCAGGGAGT 
ATTGCTGAAG TTGGAAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCGCGA 
TATCTCATCC CATGTTACTT TGATGCTATA TTAATCGGTG CATATACCAC TCTGCTGGAT 
GTAGCCTGGA AACAGATGTC AAGCTTTGTT CAGAATGGCT CGACCTTCGT GAAGCACCTG 
TCCCTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAGTGTC CTTGTCTGCC GCTGCTTTCA 
CCCTCACTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CGAAGGAGAA AGAGCAGTGC 
TGTGCTTCCT TGGCTGCAGG CTTACCTCAC TTTTCTTCTG GTCTTTTTCG CTGCTGGGGG 
AGGGATACTT TCATTGCACT TCGAGGGATG CTGCTGGTTA CTGGTCGCTA CCTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTTGTACC CTGAGGCATG GTCTCATTCC TAACCTCCTT 
GGCGAAGGAA CGTATGCCAG GTACAACTGT CGTGATGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAAGAT GGTTCCAAAT GGTCTAGACA TCCTCAAATG CCCAGTTTCC 
AGAATGTATC CAACAGATGA CTCTGCACCT TTGCCTGCTG GGACTCTGGA TCAGCCTTTG 
TTTGAAGTCA TACAGGAAGC TATGCAGAGG CACATGCAAG GCATTCAGTT CCGAGAGCGG 
AATGCCGGTC CACAGATCGA TCGAAACATG AAGGATGAGG GTTTCAACAT AACCGTGGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACATGGATG 
GATAAAATGG GAGAGAGTGA CAGAGCTAGA AACAGAGGGA TCCCAGCCAC CCCAAGAGAT 
GGGTCTGCTG TGGAAATCGT GGGCTTGTGT AAATCTGCTG TTCGTTGGTT GCTGGAATTG 
TCCAAGAAAA ACATTTTTCC TTATCATGAA GTCAGAGTAA GAAGACATGG AAAGGTTGTA 
GCAGTCTCCT ATGATGAGTG GAACAGAAAA ATACAAAACA GTTTTGAAAA GCTCTTTCAT 
GTTTCCGAAG ACCCTTCAGA TCCTAACGAG AAGCATCCGA ATCTGGTTCA CAAGCGGGGC 
ATCTACAAGG ACAGCTACGG TGCGTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCCAAC 
TTTACCATAG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CTGAAAAGGC TTGGAAGGCT 
TTGGAAATTG CAGAAGGAAA ATTGCTTGGT CCCCTTGGCA TGAAGACTTT GGATCCAGAT 
GACATGGTTT ACTGTGGAGT CTATGACAAT GCCTTAGACA ATGACAACTA CAACCTTGCC 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTATGGC CGATTGGGTA TTTTCTTCGT 
GCAAAGTTGT ATTTTTCCAA AATGATGGGC CCAGAGACTG CTGCGAAGAC TGTATTTTTG 
GTTAAAAATG TTCTTTCTAG ACATTATGTT CATCTTGAGA GATCCCCATG GAAAGGACTT 
CCAGAGCTCA CCAATGAGAA TGGCCAGTAC TGCCCTTTCA GCTGTGAGAC ACAGGCATGG 
TCGATGGCTG CTGTTCTCGA AACCCTCTAT GATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233291.1
CDS	273..4871
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGEAFNREKFRSLDWENPTEREDDSDKYCKVHLQQSGSFQYYFLQGQEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQALGLSRSCYSLADQLELNPDFSRPNRRYTWDDVGQLVEKLKREWNILCITDVVYNHTAANSKWIHEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVADGKYREKGVPALIENDHHMNCIRKIIWEDIFPRIQLWEFFQVDVQKAVEQFRRLLSQENRRVTKSEPKEHLKIIQDPEFRRLGCAVDMNIALATFIPHDNGPAAIEECCNWFRKRLEELNSEKHHLTSCHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFEEMALSAEESLLHRPDKACFLMAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATHFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPSDTGDVSIHSGIIAARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGMPNMTVELRERIQLHESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNSDPILKIPFASIASKLTLAELNQVLYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFACTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITVGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVRRHGKVVAVSYDEWNRKIQNSFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEGKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMGPETAAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSMAAVLETLYDL

Target ORF information:

RefSeq Version	XM_006233229.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233229.3

ORF Insert Sequence:

ATGGAACACA GTAAGCAGAT TCGAATTTTA CTATTGAACG AAATGGAGAA GCTAGAGAAG 
ACCCTCTTCA GACTTGAGCA AGGATTTGAA CTACAGTTCC GATTAGGCCC AACTTTACAA 
GGAAAAGCTG TTACTGTTTA TACAAATTAC CCATTTCCTG GAGAAGCATT TAATCGAGAA 
AAATTCCGTT CCTTGGATTG GGAAAACCCA ACAGAGAGAG AAGATGATTC GGACAAATAC 
TGTAAAGTTC ATCTGCAGCA GTCTGGGTCT TTTCAGTACT ACTTCCTTCA AGGACAGGAG 
AAAAGTGGCG GAGGTTACAT AGTTGTGGAT CCTATTCTGC GAGTTGGAGC TGATAATCAC 
GTGCTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CAAAGTGTTT AGGACCCTTT 
GATGAGTGGG AAAGCCGACT ACGGGTGGCA AAGGAGTCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGGCTCTCGG ACTGTCCAGG TCATGCTATT CCCTTGCCGA TCAGTTAGAA 
TTAAATCCTG ACTTCTCAAG GCCCAATAGA AGATACACCT GGGATGATGT TGGGCAGCTA 
GTAGAGAAGC TGAAGAGGGA GTGGAACATT CTTTGTATTA CCGATGTGGT CTACAATCAT 
ACTGCTGCTA ATAGTAAGTG GATCCATGAG CATCCAGAAT CTGCGTACAA TCTTGTAAAT 
TCTCCACACT TGAAACCTGC CTGGGTCCTA GACAGAGCGC TCTGGCATTT GTCCTGTGAT 
GTTGCAGATG GGAAGTACAG AGAAAAAGGA GTCCCTGCTC TAATTGAAAA TGACCATCAC 
ATGAACTGCA TTCGGAAAAT AATTTGGGAG GACATTTTCC CAAGGATTCA GCTCTGGGAG 
TTTTTCCAAG TAGATGTTCA GAAAGCGGTT GAACAATTTA GAAGACTGCT CTCTCAAGAA 
AACAGAAGAG TAACTAAGTC TGAACCGAAA GAACATCTTA AAATTATTCA GGACCCCGAA 
TTCAGGCGGC TGGGCTGTGC TGTAGATATG AACATTGCTC TGGCGACCTT TATACCACAT 
GACAATGGGC CAGCTGCAAT TGAAGAGTGC TGTAATTGGT TCCGTAAGAG ACTCGAGGAG 
TTAAATTCAG AGAAACACCA CCTCACCAGC TGTCATCAGG AACAGGCCGT TAATTGTCTT 
TTGGGAAACG TGTTTTATGA ACGGCTGGCG GGCCATGGCC CTAAACTAGG ACCCGTCACC 
AGGAAGTACC CTTTAGTTAC CAGGTATTTT ACTTTCCCCT TTGAAGAAAT GGCCTTGTCT 
GCAGAAGAGT CCCTGCTCCA CCGCCCAGAT AAAGCTTGCT TTCTGATGGC ACACAATGGC 
TGGGTGATGG GCGATGACCC GCTTCGGAAC TTTGCTGAGC CAGGTTCCAA CGTTTATTTA 
AGGAGAGAAC TTATTTGCTG GGGTGACAGT GTGAAATTGC GCTATGGGAA CAAACCGGAG 
GACTGCCCTT ACCTGTGGGC ACACATGAAG AAATACACTG AGATCACTGC CACTCACTTC 
CAGGGAGTGC GCCTTGACAA CTGCCACTCC ACGCCTCTCC ACGTGGCTGA GTACATGCTG 
GATGCTGCTA GGAAATTACA GCCCAATCTT TATGTAGTGG CTGAGCTGTT CACAGGAAGC 
GAAGATCTGG ACAATATCTT CGTTACTAGA CTGGGCATCA GTTCCTTAAT AAGAGAGGCA 
ATGAGCGCAT ATAACAGTCA CGAAGAGGGC AGATTGGTCT ACCGGTACGG AGGGGAGCCC 
GTCGGATCCT TCGTTCAGCC CTGTTTGAGG CCTCTTATGC CAGCTATCGC GCATGCCCTG 
TTTATGGACA TCACCCACGA TAATGAGTGT CCAATTGTGC ACAGATCAGC CTATGATGCG 
CTTCCAAGTA CCACAGTTGT CTCCATGGCG TGTTGTGCTA GTGGAAGTAC AAGAGGCTAC 
GACGAGCTAG TGCCTCATCA GATTTCAGTG GTTGCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGGG CATCCCCATC GGATACAGGA GATGTTAGCA TCCACAGTGG GATTATTGCA 
GCCAGATGTG CCATCAACAG ACTCCATCAA GAGCTTGGAG CCAAGGGTTT CATTCAAGTG 
TATGTGGATC AAGTGGATGA AGACATAGTG GCAGTAACCA GGCACTCACC AAGCATCCAT 
CAGTCTGTTG TGGCTGTATC GAGAACGGCT TTTAGGAATC CCAAGACTTC ATTTTACAGC 
AAAGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AGGTAGTTCT GGAAGCTAGA 
ACTATTGAGA GGAATACAAA ACCTTATAGG AAAGATGAGA ATTCAATCAA TGGAATGCCG 
AACATGACAG TGGAACTTAG AGAACGTATC CAGCTTCATG AAAGTAAAAT TGTCAAACAA 
GCTGGAGTTG CCACAAAGGG TCCCAATGAA TATATTCAGG AAATAGAATT TGAAAACCTA 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGCCTTGATC CACATGCGCA AGTTGCTGTT 
GGAATTCTTC GAAATCACCT GACCCAATTC AGCTCCCATT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT ATTAAAAATC CCCTTCGCTT CCATTGCCTC TAAATTGACT 
TTGGCTGAGC TAAATCAGGT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGACGGTGGA 
GGTTGCTATG ATATACCAAA CTGGTCGTCC CTTAAGTATG CAGGTCTGCA AGGATTAATG 
TCCGTGCTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCCTTCTG TGAAAACTTG 
AGGTCTGGAG ACTGGATGAT CGACTACGTC AGTGGCCGGC TAATCTCACG ATCAGGGAGT 
ATTGCTGAAG TTGGAAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCGCGA 
TATCTCATCC CATGTTACTT TGATGCTATA TTAATCGGTG CATATACCAC TCTGCTGGAT 
GTAGCCTGGA AACAGATGTC AAGCTTTGTT CAGAATGGCT CGACCTTCGT GAAGCACCTG 
TCCCTGGGTT CTGTCCAGAT GTGTGGAGTC GGGAAGTGTC CTTGTCTGCC GCTGCTTTCA 
CCCTCACTCC TGGATGTGCC GTGCAGGTTA AATGAGATCA CGAAGGAGAA AGAGCAGTGC 
TGTGCTTCCT TGGCTGCAGG CTTACCTCAC TTTTCTTCTG GTCTTTTTCG CTGCTGGGGG 
AGGGATACTT TCATTGCACT TCGAGGGATG CTGCTGGTTA CTGGTCGCTA CCTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTTGTACC CTGAGGCATG GTCTCATTCC TAACCTCCTT 
GGCGAAGGAA CGTATGCCAG GTACAACTGT CGTGATGCCG TGTGGTGGTG GCTGCAGTGC 
ATTCAGGACT ATTGCAAGAT GGTTCCAAAT GGTCTAGACA TCCTCAAATG CCCAGTTTCC 
AGAATGTATC CAACAGATGA CTCTGCACCT TTGCCTGCTG GGACTCTGGA TCAGCCTTTG 
TTTGAAGTCA TACAGGAAGC TATGCAGAGG CACATGCAAG GCATTCAGTT CCGAGAGCGG 
AATGCCGGTC CACAGATCGA TCGAAACATG AAGGATGAGG GTTTCAACAT AACCGTGGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAACTGTGG CACATGGATG 
GATAAAATGG GAGAGAGTGA CAGAGCTAGA AACAGAGGGA TCCCAGCCAC CCCAAGAGAT 
GGGTCTGCTG TGGAAATCGT GGGCTTGTGT AAATCTGCTG TTCGTTGGTT GCTGGAATTG 
TCCAAGAAAA ACATTTTTCC TTATCATGAA GTCAGAGTAA GAAGACATGG AAAGGTTGTA 
GCAGTCTCCT ATGATGAGTG GAACAGAAAA ATACAAAACA GTTTTGAAAA GCTCTTTCAT 
GTTTCCGAAG ACCCTTCAGA TCCTAACGAG AAGCATCCGA ATCTGGTTCA CAAGCGGGGC 
ATCTACAAGG ACAGCTACGG TGCGTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCCAAC 
TTTACCATAG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CTGAAAAGGC TTGGAAGGCT 
TTGGAAATTG CAGAAGGAAA ATTGCTTGGT CCCCTTGGCA TGAAGACTTT GGATCCAGAT 
GACATGGTTT ACTGTGGAGT CTATGACAAT GCCTTAGACA ATGACAACTA CAACCTTGCC 
AGAGGTTTCA ATTACCACCA AGGACCTGAG TGGCTATGGC CGATTGGGTA TTTTCTTCGT 
GCAAAGTTGT ATTTTTCCAA AATGATGGGC CCAGAGACTG CTGCGAAGAC TGTATTTTTG 
GTTAAAAATG TTCTTTCTAG ACATTATGTT CATCTTGAGA GATCCCCATG GAAAGGACTT 
CCAGAGCTCA CCAATGAGAA TGGCCAGTAC TGCCCTTTCA GCTGTGAGAC ACAGGCATGG 
TCGATGGCTG CTGTTCTCGA AACCCTCTAT GATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A role for AGL ubiquitination in the glycogen storage disorders of Lafora and Cori's disease.
Genes & development21(19)2399-409(2007 Oct)
Cheng A,Zhang M,Gentry MS,Worby CA,Dixon JE,Saltiel AR

Hepatic and neuromuscular forms of glycogenosis type III: nine mutations in AGL.
Human mutation27(6)600-1(2006 Jun)
Lucchiari S,Pagliarani S,Salani S,Filocamo M,Di Rocco M,Melis D,Rodolico C,Musumeci O,Toscano A,Bresolin N,Comi GP

Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum.
Acta physiologica Scandinavica181(2)239-45(2004 Jun)
Lees SJ,Chen YT,Williams JH

The genetic association database.
Nature genetics36(5)431-2(2004 May)
Becker KG,Barnes KC,Bright TJ,Wang SA

Inverse relationship of skeletal muscle glycogen from wild-type and genetically modified mice to their phosphorylase a activity.
Biochemical and biophysical research communications290(2)874-7(2002 Jan)
Schliselfeld LH,Danon MJ

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Agl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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