AGL gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017000501.1	XP_016855990.1	glycogen debranching enzyme isoform X2
XM_005270557.2	XP_005270614.1	glycogen debranching enzyme isoform X1
NM_000643.2	NP_000634.2	glycogen debranching enzyme isoform 1
NM_000646.2	NP_000637.2	glycogen debranching enzyme isoform 3
NM_000642.3	NP_000633.2	glycogen debranching enzyme isoform 1
NM_000644.2	NP_000635.2	glycogen debranching enzyme isoform 1
NM_000028.2	NP_000019.2	glycogen debranching enzyme isoform 1
NM_000642.2	NP_000633.2	glycogen debranching enzyme isoform 1
XM_005270557.1	XP_005270614.1	glycogen debranching enzyme isoform X1
XM_017000501.2	XP_016855990.1	glycogen debranching enzyme isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu83253	XM_017000501.2 Latest version!	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu16190	NM_000646.2 Latest version!	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu16218	NM_000643.2 Latest version!	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu16218	NM_000642.3 Latest version!	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu16218	NM_000028.2 Latest version!	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu16218	NM_000644.2 Latest version!	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu16218	XM_005270557.2 Latest version!	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
View Old Accession Versions >>
OHu16218	NM_000642.2	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu16218	XM_005270557.1	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu83253	XM_017000501.1	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

AGL ( XM_017000501.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855990.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83253
Clone ID Related Accession (Same CDS sequence)	XM_017000501.1 , XM_017000501.2
Accession Version	XM_017000501.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855990.1
CDS	68..2926
Translation	MSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_017000501.1
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000501.1

ORF Insert Sequence:

ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_017000501.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855990.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83253
Clone ID Related Accession (Same CDS sequence)	XM_017000501.1 , XM_017000501.2
Accession Version	XM_017000501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855990.1
CDS	69..2927
Translation	MSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_017000501.2
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000501.2

ORF Insert Sequence:

ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( NM_000646.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000637.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu16190
Clone ID Related Accession (Same CDS sequence)	NM_000646.2
Accession Version	NM_000646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84011.1 and AC096949.2. On or before Aug 31, 2013 this sequence version replaced XM_005270558.1, NM_000646.1. Transcript Variant: This variant (6) differs in the 5' UTR and coding region, compared to variant 4. The resulting protein (isoform 3) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84011.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820701, SAMN04284274 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCAA TTCTGAGCAT TAATTTATTT ATTGGGTATG AGCTACAGTT CCGATTAGGC 
CCAACTTTAC AGGGAAAAGC AGTTACCGTG TATACAAATT ACCCATTTCC TGGAGAAACA 
TTTAATAGAG AAAAATTCCG TTCTCTGGAT TGGGAAAATC CAACAGAAAG AGAAGATGAT 
TCTGATAAAT ACTGTAAACT TAATCTGCAA CAATCTGGTT CATTTCAGTA TTATTTCCTT 
CAAGGAAATG AGAAAAGTGG TGGAGGTTAC ATAGTTGTGG ACCCCATTTT ACGTGTTGGT 
GCTGATAATC ATGTGCTACC CTTGGACTGT GTTACTCTTC AGACATTTTT AGCTAAGTGT 
TTGGGACCTT TTGATGAATG GGAAAGCAGA CTTAGGGTTG CAAAAGAATC AGGCTACAAC 
ATGATTCATT TTACCCCATT GCAGACTCTT GGACTATCTA GGTCATGCTA CTCCCTTGCC 
AATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTAATA GAAAGTATAC CTGGAATGAT 
GTTGGACAGC TAGTGGAAAA ATTAAAAAAG GAATGGAATG TTATTTGTAT TACTGATGTT 
GTCTACAATC ATACTGCTGC TAATAGTAAA TGGATCCAGG AACATCCAGA ATGTGCCTAT 
AATCTTGTGA ATTCTCCACA CTTAAAACCT GCCTGGGTCT TAGACAGAGC ACTTTGGCGT 
TTCTCCTGTG ATGTTGCAGA AGGGAAATAC AAAGAAAAGG GAATACCTGC TTTGATTGAA 
AATGATCACC ATATGAATTC CATCCGAAAA ATAATTTGGG AGGATATTTT TCCAAAGCTT 
AAACTCTGGG AATTTTTCCA AGTAGATGTC AACAAAGCGG TTGAGCAATT TAGAAGACTT 
CTTACACAAG AAAATAGGCG AGTAACCAAG TCTGATCCAA ACCAACACCT TACGATTATT 
CAAGATCCTG AATACAGACG GTTTGGCTGT ACTGTAGATA TGAACATTGC ACTAACGACT 
TTCATACCAC ATGACAAGGG GCCAGCAGCA ATTGAAGAAT GCTGTAATTG GTTTCATAAA 
AGAATGGAGG AATTAAATTC AGAGAAGCAT CGACTCATTA ACTATCATCA GGAACAGGCA 
GTTAATTGCC TTTTGGGAAA TGTGTTTTAT GAACGACTGG CTGGCCATGG TCCAAAACTA 
GGACCTGTCA CTAGAAAGCA TCCTTTAGTT ACCAGGTATT TTACTTTCCC ATTTGAAGAG 
ATAGACTTCT CCATGGAAGA ATCTATGATT CATCTGCCAA ATAAAGCTTG TTTTCTGATG 
GCACACAATG GATGGGTAAT GGGAGATGAT CCTCTTCGAA ACTTTGCTGA ACCGGGTTCA 
GAAGTTTACC TAAGGAGAGA ACTTATTTGC TGGGGAGACA GTGTTAAATT ACGCTATGGG 
AATAAACCAG AGGACTGTCC TTATCTCTGG GCACACATGA AAAAATACAC TGAAATAACT 
GCAACTTATT TCCAGGGAGT ACGTCTTGAT AACTGCCACT CAACACCTCT TCACGTAGCT 
GAGTACATGT TGGATGCTGC TAGGAATTTG CAACCCAATT TATATGTAGT AGCTGAACTG 
TTCACAGGAA GTGAAGATCT GGACAATGTC TTTGTTACTA GACTGGGCAT TAGTTCCTTA 
ATAAGAGAGG CAATGAGTGC ATATAATAGT CATGAAGAGG GCAGATTAGT TTACCGATAT 
GGAGGAGAAC CTGTTGGATC CTTTGTTCAG CCCTGTTTGA GGCCTTTAAT GCCAGCTATT 
GCACATGCCC TGTTTATGGA TATTACGCAT GATAATGAGT GTCCTATTGT GCATAGATCA 
GCGTATGATG CTCTTCCAAG TACTACAATT GTTTCTATGG CATGTTGTGC TAGTGGAAGT 
ACAAGAGGCT ATGATGAATT AGTGCCTCAT CAGATTTCAG TGGTTTCTGA AGAACGGTTT 
TACACTAAGT GGAATCCTGA AGCATTGCCT TCAAACACAG GTGAAGTTAA TTTCCAAAGC 
GGCATTATTG CAGCCAGGTG TGCTATCAGT AAACTTCATC AGGAGCTTGG AGCCAAGGGT 
TTTATTCAGG TGTATGTGGA TCAAGTTGAT GAAGACATAG TGGCAGTAAC AAGACACTCA 
CCTAGCATCC ATCAGTCTGT TGTGGCTGTA TCTAGAACTG CTTTCAGGAA TCCCAAGACT 
TCATTTTACA GCAAGGAAGT GCCTCAAATG TGCATCCCTG GCAAAATTGA AGAAGTAGTT 
CTTGAAGCTA GAACTATTGA GAGAAACACG AAACCTTATA GGAAGGATGA GAATTCAATC 
AATGGAACAC CAGATATCAC AGTAGAAATT AGAGAACATA TTCAGCTTAA TGAAAGTAAA 
ATTGTTAAAC AAGCTGGAGT TGCCACAAAA GGGCCCAATG AATATATTCA AGAAATAGAA 
TTTGAAAACT TGTCTCCAGG AAGTGTTATT ATATTCAGAG TTAGTCTTGA TCCACATGCA 
CAAGTCGCTG TTGGAATTCT TCGAAATCAT CTGACACAAT TCAGTCCTCA CTTTAAATCT 
GGCAGCCTAG CTGTTGACAA TGCAGATCCT ATATTAAAAA TTCCTTTTGC TTCTCTTGCC 
TCCAGATTAA CTTTGGCTGA GCTAAATCAG ATCCTTTACC GATGTGAATC AGAAGAAAAG 
GAAGATGGTG GAGGGTGCTA TGACATACCA AACTGGTCAG CCCTTAAATA TGCAGGTCTT 
CAAGGTTTAA TGTCTGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCTTTT 
TGTAATAATT TGAGATCTGG AGATTGGATG ATTGACTATG TCAGTAACCG GCTTATTTCA 
CGATCAGGAA CTATTGCTGA AGTTGGTAAA TGGTTGCAGG CTATGTTCTT CTACCTGAAG 
CAGATCCCAC GTTACCTTAT CCCATGTTAC TTTGATGCTA TATTAATTGG TGCATATACC 
ACTCTTCTGG ATACAGCATG GAAGCAGATG TCAAGCTTTG TTCAGAATGG TTCAACCTTT 
GTGAAACACC TTTCATTGGG TTCAGTTCAA CTGTGTGGAG TAGGAAAATT CCCTTCCCTG 
CCAATTCTTT CACCTGCCCT AATGGATGTA CCTTATAGGT TAAATGAGAT CACAAAAGAA 
AAGGAGCAAT GTTGTGTTTC TCTAGCTGCA GGCTTACCTC ATTTTTCTTC TGGTATTTTC 
CGCTGCTGGG GAAGGGATAC TTTTATTGCA CTTAGAGGTA TACTGCTGAT TACTGGACGC 
TATGTAGAAG CCAGGAATAT TATTTTAGCA TTTGCGGGTA CCCTGAGGCA TGGTCTCATT 
CCTAATCTAC TGGGTGAAGG AATTTATGCC AGATACAATT GTCGGGATGC TGTGTGGTGG 
TGGCTGCAGT GTATCCAGGA TTACTGTAAA ATGGTTCCAA ATGGTCTAGA CATTCTCAAG 
TGCCCAGTTT CCAGAATGTA TCCTACAGAT GATTCTGCTC CTTTGCCTGC TGGCACACTG 
GATCAGCCAT TGTTTGAAGT CATACAGGAA GCAATGCAAA AACACATGCA GGGCATACAG 
TTCCGAGAAA GGAATGCTGG TCCCCAGATA GATCGAAACA TGAAGGACGA AGGTTTTAAT 
ATAACTGCAG GAGTTGATGA AGAAACAGGA TTTGTTTATG GAGGAAATCG TTTCAATTGT 
GGCACATGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAGAGG AATCCCAGCC 
ACACCAAGAG ATGGGTCTGC TGTGGAAATT GTGGGCCTGA GTAAATCTGC TGTTCGCTGG 
TTGCTGGAAT TATCCAAAAA AAATATTTTC CCTTATCATG AAGTCACAGT AAAAAGACAT 
GGAAAGGCTA TAAAGGTCTC ATATGATGAG TGGAACAGAA AAATACAAGA CAACTTTGAA 
AAGCTATTTC ATGTTTCCGA AGACCCTTCA GATTTAAATG AAAAGCATCC AAATCTGGTT 
CACAAACGTG GCATATACAA AGATAGTTAT GGAGCTTCAA GTCCTTGGTG TGACTATCAG 
CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTGGCCCCTG AGCTCTTTAC TACAGAAAAA 
GCATGGAAAG CTTTGGAGAT TGCAGAAAAA AAATTGCTTG GTCCCCTTGG CATGAAAACT 
TTAGATCCAG ATGATATGGT TTACTGTGGA ATTTATGACA ATGCATTAGA CAATGACAAC 
TACAATCTTG CTAAAGGTTT CAATTATCAC CAAGGACCTG AGTGGCTGTG GCCTATTGGG 
TATTTTCTTC GTGCAAAATT ATATTTTTCC AGATTGATGG GCCCGGAGAC TACTGCAAAG 
ACTATAGTTT TGGTTAAAAA TGTTCTTTCC CGACATTATG TTCATCTTGA GAGATCCCCT 
TGGAAAGGAC TTCCAGAACT GACCAATGAG AATGCCCAGT ACTGTCCTTT CAGCTGTGAA 
ACACAAGCCT GGTCAATTGC TACTATTCTT GAGACACTTT ATGATTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000637.2
CDS	260..4810
Translation	MAPILSINLFIGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	NM_000646.2
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000646.2

ORF Insert Sequence:

ATGGCCCCAA TTCTGAGCAT TAATTTATTT ATTGGGTATG AGCTACAGTT CCGATTAGGC 
CCAACTTTAC AGGGAAAAGC AGTTACCGTG TATACAAATT ACCCATTTCC TGGAGAAACA 
TTTAATAGAG AAAAATTCCG TTCTCTGGAT TGGGAAAATC CAACAGAAAG AGAAGATGAT 
TCTGATAAAT ACTGTAAACT TAATCTGCAA CAATCTGGTT CATTTCAGTA TTATTTCCTT 
CAAGGAAATG AGAAAAGTGG TGGAGGTTAC ATAGTTGTGG ACCCCATTTT ACGTGTTGGT 
GCTGATAATC ATGTGCTACC CTTGGACTGT GTTACTCTTC AGACATTTTT AGCTAAGTGT 
TTGGGACCTT TTGATGAATG GGAAAGCAGA CTTAGGGTTG CAAAAGAATC AGGCTACAAC 
ATGATTCATT TTACCCCATT GCAGACTCTT GGACTATCTA GGTCATGCTA CTCCCTTGCC 
AATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTAATA GAAAGTATAC CTGGAATGAT 
GTTGGACAGC TAGTGGAAAA ATTAAAAAAG GAATGGAATG TTATTTGTAT TACTGATGTT 
GTCTACAATC ATACTGCTGC TAATAGTAAA TGGATCCAGG AACATCCAGA ATGTGCCTAT 
AATCTTGTGA ATTCTCCACA CTTAAAACCT GCCTGGGTCT TAGACAGAGC ACTTTGGCGT 
TTCTCCTGTG ATGTTGCAGA AGGGAAATAC AAAGAAAAGG GAATACCTGC TTTGATTGAA 
AATGATCACC ATATGAATTC CATCCGAAAA ATAATTTGGG AGGATATTTT TCCAAAGCTT 
AAACTCTGGG AATTTTTCCA AGTAGATGTC AACAAAGCGG TTGAGCAATT TAGAAGACTT 
CTTACACAAG AAAATAGGCG AGTAACCAAG TCTGATCCAA ACCAACACCT TACGATTATT 
CAAGATCCTG AATACAGACG GTTTGGCTGT ACTGTAGATA TGAACATTGC ACTAACGACT 
TTCATACCAC ATGACAAGGG GCCAGCAGCA ATTGAAGAAT GCTGTAATTG GTTTCATAAA 
AGAATGGAGG AATTAAATTC AGAGAAGCAT CGACTCATTA ACTATCATCA GGAACAGGCA 
GTTAATTGCC TTTTGGGAAA TGTGTTTTAT GAACGACTGG CTGGCCATGG TCCAAAACTA 
GGACCTGTCA CTAGAAAGCA TCCTTTAGTT ACCAGGTATT TTACTTTCCC ATTTGAAGAG 
ATAGACTTCT CCATGGAAGA ATCTATGATT CATCTGCCAA ATAAAGCTTG TTTTCTGATG 
GCACACAATG GATGGGTAAT GGGAGATGAT CCTCTTCGAA ACTTTGCTGA ACCGGGTTCA 
GAAGTTTACC TAAGGAGAGA ACTTATTTGC TGGGGAGACA GTGTTAAATT ACGCTATGGG 
AATAAACCAG AGGACTGTCC TTATCTCTGG GCACACATGA AAAAATACAC TGAAATAACT 
GCAACTTATT TCCAGGGAGT ACGTCTTGAT AACTGCCACT CAACACCTCT TCACGTAGCT 
GAGTACATGT TGGATGCTGC TAGGAATTTG CAACCCAATT TATATGTAGT AGCTGAACTG 
TTCACAGGAA GTGAAGATCT GGACAATGTC TTTGTTACTA GACTGGGCAT TAGTTCCTTA 
ATAAGAGAGG CAATGAGTGC ATATAATAGT CATGAAGAGG GCAGATTAGT TTACCGATAT 
GGAGGAGAAC CTGTTGGATC CTTTGTTCAG CCCTGTTTGA GGCCTTTAAT GCCAGCTATT 
GCACATGCCC TGTTTATGGA TATTACGCAT GATAATGAGT GTCCTATTGT GCATAGATCA 
GCGTATGATG CTCTTCCAAG TACTACAATT GTTTCTATGG CATGTTGTGC TAGTGGAAGT 
ACAAGAGGCT ATGATGAATT AGTGCCTCAT CAGATTTCAG TGGTTTCTGA AGAACGGTTT 
TACACTAAGT GGAATCCTGA AGCATTGCCT TCAAACACAG GTGAAGTTAA TTTCCAAAGC 
GGCATTATTG CAGCCAGGTG TGCTATCAGT AAACTTCATC AGGAGCTTGG AGCCAAGGGT 
TTTATTCAGG TGTATGTGGA TCAAGTTGAT GAAGACATAG TGGCAGTAAC AAGACACTCA 
CCTAGCATCC ATCAGTCTGT TGTGGCTGTA TCTAGAACTG CTTTCAGGAA TCCCAAGACT 
TCATTTTACA GCAAGGAAGT GCCTCAAATG TGCATCCCTG GCAAAATTGA AGAAGTAGTT 
CTTGAAGCTA GAACTATTGA GAGAAACACG AAACCTTATA GGAAGGATGA GAATTCAATC 
AATGGAACAC CAGATATCAC AGTAGAAATT AGAGAACATA TTCAGCTTAA TGAAAGTAAA 
ATTGTTAAAC AAGCTGGAGT TGCCACAAAA GGGCCCAATG AATATATTCA AGAAATAGAA 
TTTGAAAACT TGTCTCCAGG AAGTGTTATT ATATTCAGAG TTAGTCTTGA TCCACATGCA 
CAAGTCGCTG TTGGAATTCT TCGAAATCAT CTGACACAAT TCAGTCCTCA CTTTAAATCT 
GGCAGCCTAG CTGTTGACAA TGCAGATCCT ATATTAAAAA TTCCTTTTGC TTCTCTTGCC 
TCCAGATTAA CTTTGGCTGA GCTAAATCAG ATCCTTTACC GATGTGAATC AGAAGAAAAG 
GAAGATGGTG GAGGGTGCTA TGACATACCA AACTGGTCAG CCCTTAAATA TGCAGGTCTT 
CAAGGTTTAA TGTCTGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCTTTT 
TGTAATAATT TGAGATCTGG AGATTGGATG ATTGACTATG TCAGTAACCG GCTTATTTCA 
CGATCAGGAA CTATTGCTGA AGTTGGTAAA TGGTTGCAGG CTATGTTCTT CTACCTGAAG 
CAGATCCCAC GTTACCTTAT CCCATGTTAC TTTGATGCTA TATTAATTGG TGCATATACC 
ACTCTTCTGG ATACAGCATG GAAGCAGATG TCAAGCTTTG TTCAGAATGG TTCAACCTTT 
GTGAAACACC TTTCATTGGG TTCAGTTCAA CTGTGTGGAG TAGGAAAATT CCCTTCCCTG 
CCAATTCTTT CACCTGCCCT AATGGATGTA CCTTATAGGT TAAATGAGAT CACAAAAGAA 
AAGGAGCAAT GTTGTGTTTC TCTAGCTGCA GGCTTACCTC ATTTTTCTTC TGGTATTTTC 
CGCTGCTGGG GAAGGGATAC TTTTATTGCA CTTAGAGGTA TACTGCTGAT TACTGGACGC 
TATGTAGAAG CCAGGAATAT TATTTTAGCA TTTGCGGGTA CCCTGAGGCA TGGTCTCATT 
CCTAATCTAC TGGGTGAAGG AATTTATGCC AGATACAATT GTCGGGATGC TGTGTGGTGG 
TGGCTGCAGT GTATCCAGGA TTACTGTAAA ATGGTTCCAA ATGGTCTAGA CATTCTCAAG 
TGCCCAGTTT CCAGAATGTA TCCTACAGAT GATTCTGCTC CTTTGCCTGC TGGCACACTG 
GATCAGCCAT TGTTTGAAGT CATACAGGAA GCAATGCAAA AACACATGCA GGGCATACAG 
TTCCGAGAAA GGAATGCTGG TCCCCAGATA GATCGAAACA TGAAGGACGA AGGTTTTAAT 
ATAACTGCAG GAGTTGATGA AGAAACAGGA TTTGTTTATG GAGGAAATCG TTTCAATTGT 
GGCACATGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAGAGG AATCCCAGCC 
ACACCAAGAG ATGGGTCTGC TGTGGAAATT GTGGGCCTGA GTAAATCTGC TGTTCGCTGG 
TTGCTGGAAT TATCCAAAAA AAATATTTTC CCTTATCATG AAGTCACAGT AAAAAGACAT 
GGAAAGGCTA TAAAGGTCTC ATATGATGAG TGGAACAGAA AAATACAAGA CAACTTTGAA 
AAGCTATTTC ATGTTTCCGA AGACCCTTCA GATTTAAATG AAAAGCATCC AAATCTGGTT 
CACAAACGTG GCATATACAA AGATAGTTAT GGAGCTTCAA GTCCTTGGTG TGACTATCAG 
CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTGGCCCCTG AGCTCTTTAC TACAGAAAAA 
GCATGGAAAG CTTTGGAGAT TGCAGAAAAA AAATTGCTTG GTCCCCTTGG CATGAAAACT 
TTAGATCCAG ATGATATGGT TTACTGTGGA ATTTATGACA ATGCATTAGA CAATGACAAC 
TACAATCTTG CTAAAGGTTT CAATTATCAC CAAGGACCTG AGTGGCTGTG GCCTATTGGG 
TATTTTCTTC GTGCAAAATT ATATTTTTCC AGATTGATGG GCCCGGAGAC TACTGCAAAG 
ACTATAGTTT TGGTTAAAAA TGTTCTTTCC CGACATTATG TTCATCTTGA GAGATCCCCT 
TGGAAAGGAC TTCCAGAACT GACCAATGAG AATGCCCAGT ACTGTCCTTT CAGCTGTGAA 
ACACAAGCCT GGTCAATTGC TACTATTCTT GAGACACTTT ATGATTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( NM_000643.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000634.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu16218
Clone ID Related Accession (Same CDS sequence)	XM_005270557.2 , NM_000643.2 , NM_000642.3 , NM_000644.2 , NM_000028.2 , NM_000642.2 , XM_005270557.1
Accession Version	NM_000643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569081600000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84009.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced NM_000643.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000634.2
CDS	199..4797
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	NM_000643.2
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000643.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( NM_000642.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000633.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.

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CloneID	OHu16218
Clone ID Related Accession (Same CDS sequence)	XM_005270557.2 , NM_000643.2 , NM_000642.3 , NM_000644.2 , NM_000028.2 , NM_000642.2 , XM_005270557.1
Accession Version	NM_000642.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84007.1, BC078663.1, AC096949.2 and AK130308.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000642.2. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84007.1, SRR1660809.258405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361915.8/ ENSP00000355106.3 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000633.2
CDS	124..4722
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	NM_000642.3
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000642.3

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( NM_000644.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000635.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 2, mRNA.

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CloneID	OHu16218
Clone ID Related Accession (Same CDS sequence)	XM_005270557.2 , NM_000643.2 , NM_000642.3 , NM_000644.2 , NM_000028.2 , NM_000642.2 , XM_005270557.1
Accession Version	NM_000644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84008.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced NM_000644.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267751, SAMN03267756 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000635.2
CDS	139..4737
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	NM_000644.2
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000644.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( NM_000028.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000019.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4, mRNA.

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CloneID	OHu16218
Clone ID Related Accession (Same CDS sequence)	XM_005270557.2 , NM_000643.2 , NM_000642.3 , NM_000644.2 , NM_000028.2 , NM_000642.2 , XM_005270557.1
Accession Version	NM_000028.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84010.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced NM_000028.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (1). Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000019.2
CDS	479..5077
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	NM_000028.2
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000028.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( NM_000642.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000633.2 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.

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CloneID	OHu16218
Clone ID Related Accession (Same CDS sequence)	XM_005270557.2 , NM_000643.2 , NM_000642.3 , NM_000644.2 , NM_000028.2 , NM_000642.2 , XM_005270557.1
Accession Version	NM_000642.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84007.1, BC078663.1, AC096949.2, AK130308.1 and C00487.1. This sequence is a reference standard in the RefSeqGene project. On Oct 27, 2006 this sequence version replaced NM_000642.1. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84007.1, SRR1660809.258405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000633.2
CDS	401..4999
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	NM_000642.2
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000642.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005270557.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270614.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

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CloneID	OHu16218
Clone ID Related Accession (Same CDS sequence)	XM_005270557.2 , NM_000643.2 , NM_000642.3 , NM_000644.2 , NM_000028.2 , NM_000642.2 , XM_005270557.1
Accession Version	XM_005270557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270614.1
CDS	94..4692
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_005270557.2
Organism	Homo sapiens(human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270557.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005270557.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270614.1 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu16218
Clone ID Related Accession (Same CDS sequence)	XM_005270557.2 , NM_000643.2 , NM_000642.3 , NM_000644.2 , NM_000028.2 , NM_000642.2 , XM_005270557.1
Accession Version	XM_005270557.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426003200000
Organism	Homo sapiens(Human)
Product	glycogen debranching enzyme isoform X1
Comment

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005270614.1

Translation

MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_005270557.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270557.1

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA 
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT 
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT 
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT 
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA 
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT 
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA 
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT 
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC 
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA 
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC 
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG 
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT 
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA 
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT 
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA 
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT 
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT 
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT 
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA 
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT 
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT 
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA 
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG 
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG 
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA 
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA 
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT 
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG 
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AGL
Entrez Gene ID	178
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Synonyms	GDE
General protein information	Preferred Names amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Names glycogen debranching enzyme amylo-1, 6-glucosidase, 4-alpha-glucanotransferase amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferaseprovided glycogen debrancher glycogen debranching protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p21.2
Summary	This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	610860
Disorder Html:	Glycogen storage disease IIIa, 232400 (3); Glycogen storage disease IIIb, 232400 (3)

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Homo sapiens (human)	AGL	NP_000635.2
Macaca mulatta (Rhesus monkey)	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AGL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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