AGL cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGL
Entrez Gene ID	479931
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
General protein information	Preferred Names amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Names glycogen debranching enzyme amylo-1, 6-glucosidase, 4-alpha-glucanotransferase glycogen debrancher
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005621311.2	XP_005621368.1	glycogen debranching enzyme isoform X1
XM_005621311.3	XP_005621368.1	glycogen debranching enzyme isoform X1
NM_001048096.1	NP_001041561.1	glycogen debranching enzyme
XM_005621312.2	XP_005621369.1	glycogen debranching enzyme isoform X1
XM_005621310.2	XP_005621367.1	glycogen debranching enzyme isoform X1
XM_005621312.3	XP_005621369.1	glycogen debranching enzyme isoform X1
XM_005621310.3	XP_005621367.1	glycogen debranching enzyme isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Homo sapiens (human)	AGL	NP_000635.2
Macaca mulatta (Rhesus monkey)	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Gene Ontology

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe33753	NM_001048096.1 Latest version!	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCe07839	XM_005621310.3 Latest version!	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCe07839	XM_005621312.3 Latest version!	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCe07839	XM_005621311.3 Latest version!	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCe07839	XM_005621311.2	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCe07839	XM_005621312.2	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCe07839	XM_005621310.2	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGL ( NM_001048096.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001041561.1 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.

Price & Availability↑

CloneID	OCe33753
Clone ID Related Accession (Same CDS sequence)	NM_001048096.1
Accession Version	NM_001048096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Canis lupus familiaris(dog)
Product	glycogen debranching enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ307574.1. On Sep 15, 2006 this sequence version replaced XM_537057.2. ##Evidence-Data-START## Transcript exon combination :: DQ307574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT GTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTYC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041561.1
CDS	171..4772
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILYVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNKKYTWSDVGQLVEKMKKEWNVLCITDVVYNHTAANSKWIQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKEKGVPALIENDHQMNCIRKIIWEDIFPKIQLWEFFQVDVYKAVEQFRRLLTQENRKITTKPDPKEHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFRKRIEELNSEKHQLVNYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFEEMTVSTEESMIHNPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSEYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPGASPSNTGEVNFQSGIIAARCAINKLHQELGAQGFIQVYVDQVDEDIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYQKDKNSINGMPNITVEIREHIQLSESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVENSDPILKIPFAFIASKLTLAELNQVLYRCEAEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPSLPLLSPSLMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSVPLSAGTLDQPLFEVIQEVMQRHIQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVRWLLELSKKRIFPYHEVRVKRHGKVVTISYDEWNKKIQDNFEKLFHVSEDPnullDFNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMGPEANAKTVFLVKNILSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	NM_001048096.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001048096.1

ORF Insert Sequence:

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT GTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTYC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005621311.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621368.1 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe07839
Clone ID Related Accession (Same CDS sequence)	XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
Accession Version	XM_005621311.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	glycogen debranching enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621311.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621368.1
CDS	348..4949
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILYVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNKKYTWSDVGQLVEKMKKEWNVLCITDVVYNHTAANSKWIQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKEKGVPALIENDHQMNCIRKIIWEDIFPKIQLWEFFQVDVYKAVEQFRRLLTQENRKITTKPDPKEHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFRKRIEELNSEKHQLVNYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFEEMTVSTEESMIHNPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSEYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPGASPSNTGEVNFQSGIIAARCAINKLHQELGAQGFIQVYVDQVDEDIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYQKDKNSINGMPNITVEIREHIQLSESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVENSDPILKIPFAFIASKLTLAELNQVLYRCEAEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPSLPLLSPSLMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSVPLSAGTLDQPLFEVIQEVMQRHIQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVRWLLELSKKRIFPYHEVRVKRHGKVVTISYDEWNKKIQDNFEKLFHVSEDPSDFNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMGPEANAKTVFLVKNILSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_005621311.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621311.2

ORF Insert Sequence:

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005621311.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621368.1 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

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CloneID	OCe07839
Clone ID Related Accession (Same CDS sequence)	XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
Accession Version	XM_005621311.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621311.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621368.1
CDS	351..4952
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILYVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNKKYTWSDVGQLVEKMKKEWNVLCITDVVYNHTAANSKWIQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKEKGVPALIENDHQMNCIRKIIWEDIFPKIQLWEFFQVDVYKAVEQFRRLLTQENRKITTKPDPKEHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFRKRIEELNSEKHQLVNYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFEEMTVSTEESMIHNPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSEYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPGASPSNTGEVNFQSGIIAARCAINKLHQELGAQGFIQVYVDQVDEDIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYQKDKNSINGMPNITVEIREHIQLSESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVENSDPILKIPFAFIASKLTLAELNQVLYRCEAEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPSLPLLSPSLMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSVPLSAGTLDQPLFEVIQEVMQRHIQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVRWLLELSKKRIFPYHEVRVKRHGKVVTISYDEWNKKIQDNFEKLFHVSEDPSDFNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMGPEANAKTVFLVKNILSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_005621311.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621311.3

ORF Insert Sequence:

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005621312.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621369.1 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

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CloneID	OCe07839
Clone ID Related Accession (Same CDS sequence)	XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
Accession Version	XM_005621312.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	glycogen debranching enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621369.1
CDS	228..4829
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILYVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNKKYTWSDVGQLVEKMKKEWNVLCITDVVYNHTAANSKWIQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKEKGVPALIENDHQMNCIRKIIWEDIFPKIQLWEFFQVDVYKAVEQFRRLLTQENRKITTKPDPKEHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFRKRIEELNSEKHQLVNYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFEEMTVSTEESMIHNPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSEYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPGASPSNTGEVNFQSGIIAARCAINKLHQELGAQGFIQVYVDQVDEDIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYQKDKNSINGMPNITVEIREHIQLSESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVENSDPILKIPFAFIASKLTLAELNQVLYRCEAEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPSLPLLSPSLMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSVPLSAGTLDQPLFEVIQEVMQRHIQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVRWLLELSKKRIFPYHEVRVKRHGKVVTISYDEWNKKIQDNFEKLFHVSEDPSDFNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMGPEANAKTVFLVKNILSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_005621312.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621312.2

ORF Insert Sequence:

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005621310.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621367.1 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

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CloneID	OCe07839
Clone ID Related Accession (Same CDS sequence)	XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
Accession Version	XM_005621310.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	glycogen debranching enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621367.1
CDS	175..4776
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILYVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNKKYTWSDVGQLVEKMKKEWNVLCITDVVYNHTAANSKWIQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKEKGVPALIENDHQMNCIRKIIWEDIFPKIQLWEFFQVDVYKAVEQFRRLLTQENRKITTKPDPKEHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFRKRIEELNSEKHQLVNYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFEEMTVSTEESMIHNPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSEYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPGASPSNTGEVNFQSGIIAARCAINKLHQELGAQGFIQVYVDQVDEDIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYQKDKNSINGMPNITVEIREHIQLSESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVENSDPILKIPFAFIASKLTLAELNQVLYRCEAEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPSLPLLSPSLMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSVPLSAGTLDQPLFEVIQEVMQRHIQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVRWLLELSKKRIFPYHEVRVKRHGKVVTISYDEWNKKIQDNFEKLFHVSEDPSDFNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMGPEANAKTVFLVKNILSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_005621310.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621310.2

ORF Insert Sequence:

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005621312.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621369.1 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

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CloneID	OCe07839
Clone ID Related Accession (Same CDS sequence)	XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
Accession Version	XM_005621312.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621312.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621369.1
CDS	113..4714
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILYVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNKKYTWSDVGQLVEKMKKEWNVLCITDVVYNHTAANSKWIQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKEKGVPALIENDHQMNCIRKIIWEDIFPKIQLWEFFQVDVYKAVEQFRRLLTQENRKITTKPDPKEHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFRKRIEELNSEKHQLVNYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFEEMTVSTEESMIHNPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSEYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPGASPSNTGEVNFQSGIIAARCAINKLHQELGAQGFIQVYVDQVDEDIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYQKDKNSINGMPNITVEIREHIQLSESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVENSDPILKIPFAFIASKLTLAELNQVLYRCEAEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPSLPLLSPSLMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSVPLSAGTLDQPLFEVIQEVMQRHIQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVRWLLELSKKRIFPYHEVRVKRHGKVVTISYDEWNKKIQDNFEKLFHVSEDPSDFNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMGPEANAKTVFLVKNILSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_005621312.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621312.3

ORF Insert Sequence:

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_005621310.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621367.1 Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

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CloneID	OCe07839
Clone ID Related Accession (Same CDS sequence)	XM_005621311.2 , XM_005621311.3 , XM_005621312.2 , XM_005621310.2 , XM_005621312.3 , XM_005621310.3
Accession Version	XM_005621310.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621367.1
CDS	175..4776
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQAGSFQYYFLQGNEKSGGGYIVVDPILYVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPNKKYTWSDVGQLVEKMKKEWNVLCITDVVYNHTAANSKWIQEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVAEGKYKEKGVPALIENDHQMNCIRKIIWEDIFPKIQLWEFFQVDVYKAVEQFRRLLTQENRKITTKPDPKEHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFRKRIEELNSEKHQLVNYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFEEMTVSTEESMIHNPNKACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSEYDALPSTTIVSMACCASGSTKGYDELVPHQISVVSEERFYTKWNPGASPSNTGEVNFQSGIIAARCAINKLHQELGAQGFIQVYVDQVDEDIVAVTRHSPSIHQSVVSVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYQKDKNSINGMPNITVEIREHIQLSESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVENSDPILKIPFAFIASKLTLAELNQVLYRCEAEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPSLPLLSPSLMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSVPLSAGTLDQPLFEVIQEVMQRHIQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVRWLLELSKKRIFPYHEVRVKRHGKVVTISYDEWNKKIQDNFEKLFHVSEDPSDFNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMGPEANAKTVFLVKNILSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_005621310.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621310.3

ORF Insert Sequence:

ATGGGACACA GTAAGCAGAT TAGAATCTTA CTTCTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTTCGAG CTACAGTTCC GATTAGGCCC CACTTTACAG 
GGAAAAGCAG TTACTGTGTA CACAAATTAC CCATTTCCCG GAGAAACATT TAATCGAGAA 
AAATTCCGTT CCCTGGAATG GGAAAACCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAGCA AGCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GGGGTTACAT AGTTGTGGAT CCTATTTTAT ATGTTGGTGC TGATAATCAC 
GTATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGAGTTGCA AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTAC AGACTCTTGG GCTATCTAGA TCATGCTATT CTCTTGCCAA CCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTAATAAA AAATATACCT GGAGTGATGT TGGGCAACTA 
GTGGAAAAAA TGAAAAAGGA ATGGAATGTT CTTTGTATTA CTGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAA GTGCATATAA CCTTGTAAAT 
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT ATCATGTGAC 
GTTGCAGAAG GGAAATACAA AGAAAAAGGA GTACCTGCTT TGATTGAAAA TGATCATCAA 
ATGAATTGCA TTCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGATTCA ACTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
AATAGGAAAA TAACAACCAA GCCTGATCCA AAGGAACACC TTAAGATTAT TCAGGATCCT 
GAATACAGAA GACTTGGCTG CACTGTAGAT ATGAACATTG CACTAGCAAC TTTCATACCA 
CATGACAAGG GCCCAGCTGC AATCGATGAA TGCTGTAATT GGTTCCGTAA GAGAATTGAG 
GAATTAAATT CAGAGAAGCA TCAACTCGTG AATTATCATC AGGAACAGGC AGTTAATTGC 
CTTTTGGGAA ATGTGTTTTA TGAACGAATG GCTGGCCACG GTCCTAAATT AGGACCTGTC 
ACTAGAAAAC ATCCATTAGT TACCAGATAT TTTACGTTCC CATTTGAAGA AATGACCGTC 
TCCACAGAAG AGTCTATGAT TCATAACCCA AATAAAGCTT GTTTTCTGAT GGCCCATAAT 
GGATGGGTAA TGGGAGATGA TCCCCTTCGA AACTTTGCTG AACCAGGTTC AGAAGTTTAC 
CTAAGGAGAG AGCTTATTTG CTGGGGAGAC AGTGTTAAAT TACGCTATGG GAATAAACCA 
GAGGACTGTC CTTATCTCTG GGCACACATG AAAAAATACA CTGAAATAAC TGCAACCTAT 
TTCCAGGGAG TACGTCTTGA TAACTGCCAC TCAACGCCTC TTCATGTAGC TGAGTACATG 
TTGGATGCTG CTAGGAAATT GCAACCCAAT TTGTATGTAG TAGCTGAACT GTTCACAGGA 
AGCGAAGACC TGGACAACAT CTTTGTTACT AGACTTGGCA TTAGTTCCCT AATAAGAGAG 
GCAATGAGTG CATATAATAG CCATGAAGAG GGCAGATTAG TTTACAGATA TGGAGGAGAA 
CCTGTTGGAT CCTTTGTTCA GCCCTGTTTG AGGCCACTAA TGCCAGCTAT TGCACATGCT 
CTGTTTATGG ATATTACCCA CGATAATGAG TGTCCTATTG TGCATAGGTC AGAATATGAT 
GCTCTCCCAA GTACCACGAT TGTTTCTATG GCATGTTGTG CTAGTGGAAG TACTAAAGGC 
TATGATGAAT TAGTGCCTCA TCAGATTTCA GTGGTTTCTG AAGAACGGTT TTATACTAAG 
TGGAATCCTG GAGCATCTCC ATCAAATACA GGTGAAGTTA ATTTCCAAAG TGGAATTATT 
GCAGCCAGAT GTGCTATCAA TAAACTTCAT CAGGAGCTTG GGGCCCAGGG TTTCATTCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATT GTGGCAGTAA CAAGACATTC ACCCAGTATC 
CATCAGTCTG TTGTGTCTGT TTCTAGAACT GCTTTCAGGA ATCCTAAGAC TTCATTTTAC 
AGCAAGGAAG TGCCTCAAAT GTGCATCCCT GGCAAAATTG AAGAAGTAGT TCTTGAAGCT 
AGAACTATTG AGAGAAATAC AAAACCTTAT CAGAAGGATA AGAATTCGAT CAATGGAATG 
CCAAATATCA CAGTAGAAAT TAGAGAACAT ATTCAGCTTA GTGAAAGTAA AATTGTTAAG 
CAAGCTGGAG TTGCCACAAA AGGACCCAAT GAATATATTC AAGAAATAGA ATTTGAAAAC 
TTGTCTCCAG GAAGTGTCAT TATATTCAGA GTTAGTCTTG ATCCACATGC ACAAGTCGCT 
GTTGGAATTC TTCGAAATCA TCTGACACAG TTTAGTCCTC ACTTTAAATC TGGAAGCCTT 
GCTGTTGAAA ACTCAGATCC TATATTAAAA ATTCCTTTTG CTTTTATTGC CTCAAAATTA 
ACTTTGGCTG AGCTGAATCA GGTACTTTAC CGATGTGAAG CAGAAGAACA AGAAGATGGT 
GGAGGCTGTT ATGACATACC AAACTGGTCA TCTCTTAAAT ATGCAGGTCT CCAAGGGTTA 
ATGTCCGTCT TGGCAGAAAT AAGGCCAAAG AATGACTTGG GGCATCCCTT TTGTGATAAT 
TTGAGATCTG GAGATTGGAT GATTGACTAT GTCAGTAACC GGCTTATCTC TCGATCAGGA 
ACTATTGCAG AAGTTGGTAA ATGGTTCCAG GCTATGTTCT TCTACCTGAA GCAAATCCCA 
CGTTATCTTA TCCCATGTTA TTTTGATGCT ATATTAATTG GTGCATACAC CACTCTTCTG 
GATATAGCAT GGAAGCAGAT GTCAAGCTTT GTTCAGAATG GTTCTACCTT TGTGAAACAC 
CTTTCACTGG GATCAGTGCA AATGTGTGGA GTAGGAAAGT GCCCTTCTCT GCCACTTCTT 
TCACCTTCAC TTATGGATGT ACCATATAGG CTAAATGAGA TCACAAAAGA AAAGGAGCAA 
TGTTGTGTGT CTCTAGCTGC AGGCTTACCT CATTTTTCTT CTGGTATTTT CCGCTGTTGG 
GGAAGGGATA CTTTTATTGC ACTGAGAGGT CTACTGCTGA TTACTGGACG CTATTTAGAG 
GCCAGGAATA TTATTTTAGC ATTTGCTGGT ACCCTGAGAC ACGGTCTCAT TCCTAATCTC 
CTGGGTGAAG GAACTTATGC CAGATACAAT TGCCGGGATG CTGTGTGGTG GTGGCTACAG 
TGTATTCAGG ATTACTGTAA AATGGTTCCA AATGGCCTAG ACATTCTCAA GTGCCCAGTT 
TCCAGAATGT ATCCTACAGA TGATTCTGTT CCTTTGTCTG CCGGCACACT GGATCAACCA 
CTGTTTGAAG TAATACAGGA AGTCATGCAA AGACACATAC AGGGCATACA ATTCCGAGAG 
AGGAATGCTG GTCCACAAAT AGACCGAAAC ATGAAGGACG AAGGCTTTAA TATAACTGCA 
GGGGTTGACG AAGAAACAGG ATTTGTTTAT GGAGGAAATC GCTTAAACTG TGGCACATGG 
ATGGATAAAA TGGGAGAAAG TGATAGAGCT AGAAACAGAG GAATCCCAGC CACTCCAAGA 
GATGGGTCTG CTGTGGAAAT TGTGGGCCTG AGTAAATCTA CTGTCCGCTG GTTGCTGGAA 
TTATCCAAAA AAAGAATTTT CCCTTATCAT GAAGTCAGAG TAAAAAGACA TGGAAAGGTT 
GTGACAATCT CATATGATGA GTGGAACAAA AAAATACAAG ACAACTTTGA AAAGCTATTT 
CATGTGTCAG AAGACCCTTC AGATTTTAAT GAAAAGCATC CTAACCTGGT TCACAAACGT 
GGCATATACA AAGATAGTTA TGGAGCTTCC AGCCCTTGGT GTGACTATCA GCTCAGGCCT 
AATTTTACCA TAGCAATGGT TGTGGCCCCT GAGCTCTTTA CTGCAGAGAA AGCATGGAAA 
GCTCTGGAGA TTGCAGAAAA AAAGCTGCTT GGTCCCCTAG GCATGAAAAC TTTGGATCCA 
GACGATATGG TTTATTGTGG AATTTATGAC AATGCCTTAG ACAATGACAA CTACAATCTT 
GCTAAAGGTT TCAATTATCA CCAAGGACCT GAGTGGCTGT GGCCCGTTGG GTATTTTCTT 
CGTGCAAAAT TATATTTTTC TAAATTGATG GGTCCAGAGG CCAATGCCAA GACTGTGTTT 
TTGGTTAAAA ACATTCTTTC CCGACATTAT GTTCATCTTG AGAGATCCCC TTGGAAAGGA 
CTTCCTGAAC TGACCAATGA GAATGGCCAA TACTGTCCTT TCAGCTGTGA AACACAAGCC 
TGGTCAATCG CTACTGTTCT TGAAACACTC TATGACTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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AGL cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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