Ube4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube4a
Entrez Gene ID	315608
Full Name	ubiquitination factor E4A
Synonyms	Ab2-232
General protein information	Preferred Names ubiquitination factor E4A Names ubiquitin conjugation factor E4 A RING-type E3 ubiquitin transferase E4 A ubiquitination factor E4A (UFD2 homolog, yeast) ubiquitination factor E4A, UFD2 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_006242938.3	XP_006243000.1	ubiquitin conjugation factor E4 A isoform X1
NM_207610.1	NP_997493.1	ubiquitin conjugation factor E4 A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001681	XP_321414.5
Homo sapiens (human)	UBE4A	NP_004779.2
Bos taurus (cattle)	UBE4A	NP_001092469.1
Pan troglodytes (chimpanzee)	UBE4A	XP_508791.2
Xenopus tropicalis (tropical clawed frog)	ube4a	NP_001163971.1
Canis lupus familiaris (dog)	UBE4A	XP_850081.1
Mus musculus (house mouse)	Ube4a	NP_663375.3
Danio rerio (zebrafish)	ube4a	XP_001922961.2
Drosophila melanogaster (fruit fly)	CG11070	NP_609060.1
Macaca mulatta (Rhesus monkey)	UBE4A	XP_001097911.2
Rattus norvegicus (Norway rat)	Ube4a	NP_997493.1
Gallus gallus (chicken)	UBE4A	NP_001026081.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression analysis of the gene encoding for the U-box-type ubiquitin ligase UBE4A in human tissues.
Contino G, Amati F, Pucci S, Pontieri E, Pichiorri F, Novelli A, Botta A, Mango R, Nardone AM, Sangiuolo FC, Citro G, Spagnoli LG, Novelli G
Gene32869-74(2004 Mar)

U box proteins as a new family of ubiquitin-protein ligases.
Hatakeyama S, Yada M, Matsumoto M, Ishida N, Nakayama KI
The Journal of biological chemistry276(35)33111-20(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05613	NM_207610.1 Latest version!	Rattus norvegicus ubiquitination factor E4A (Ube4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa31082	XM_006242938.3 Latest version!	Rattus norvegicus ubiquitination factor E4A (Ube4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube4a ( NM_207610.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997493.1 Rattus norvegicus ubiquitination factor E4A (Ube4a), mRNA.

Price & Availability↑

CloneID	ORa05613
Clone ID Related Accession (Same CDS sequence)	NM_207610.1
Accession Version	NM_207610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550160000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin conjugation factor E4 A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061761.1. On Aug 30, 2004 this sequence version replaced XM_236195.2. ##Evidence-Data-START## Transcript exon combination :: BC061761.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA GTTTGCAGCA ATCCACAAAG AGCAGCTGAA ACAGCAGTCT 
GATGAGCTCC CAGCAAGCCC AGATGACTCA GATAATAGTG TGTCGGAGAG CCTGGATGAG 
TTTGACTACT CAGTGTCTGA GATCAGCCGC TCTTTCCGGA CACATCAAGA AATGTGTGAA 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTCA TTACTCTGGA CAACAGTGAT 
CCAAACTTGA AAAGTGGGAA CGGCATCCCC AGTCGGTGCG TGTACTTGGA AGAAATGGCA 
GTGGAGCTGG AAGATCAAGA CTGGCTGGAT ATGAGCAACG TGGAGCAGGC CATCTTTGCT 
CGGTTACTGC TTCAAGATCC AGGAAACCAC TTGATCAGCA TGACTTCCTC TGCAACTTTA 
AACCTCTCTG CTGATCGCGA TGCAGGAGAG CGGCACATCT TCTGTTACCT TTACTCCTGC 
TTCCAAAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAAA ACCTACTCCC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAACACG CGAACAGTTC TCCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GACTTGATGT TGGAAGCTAT TCAGGGAGCC 
CATTTTGAAG ATGTGACTGA GTTTTTAGAA GAGGTCATTG AAGCCTTGCT ATTGGATGAG 
GAGGTTCGAA CATTTCCTGA AGTCATGATT CCAGTGTTTG ATATTTTACT TGGCCGAATA 
AAAGATCTGG AACTCTGCCA GATCCTCCTG TACGCATACC TGGACATCCT TCTCTATTTC 
ACCAGGCAGA AGGATATGGC CAAGGTTTTT TTAGAGTACA TTCAGCCCAA GGACCCTAGC 
AATGGGCAGA TGTATCAGAA GACCTTGCTG GGAGTCATTC TGAATATCTC GTGCTTGTTA 
AAGACTCCGG GTGTGGTAGA GAATCATGGC TTCTTCCTGA ATCCATCCCG TTCCAGTCCT 
CAGGAGATCA AAGTACAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCATGAAAAG 
ATCTACCAGA TGCTGAAGAA TCTGCTCCAG CTCTCTCCAG AAACCAAGCA TGGTATCTTG 
TTCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATCTG GGCCAATCAG 
ATGCCCGAAA TCTTCTTCCA AATGTATGCC TCGGATGCCT TCTTTCTGAA TCTGGGTGCT 
GCTCTCCTGA AGCTGTGCCA GCCATTCTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AACCCCACAT ACTGTGTCCT CAAGGACTTG AATGATGAAG AACGAAAAAT TAAAAGTGTA 
CATATGAGAG GTTTAGACAA GGAGACCTGT CTGATCCCAG CTGTGCAGGA GCCAGTGTTC 
CCCCAGAGCT ACAATCTGGT GACCGAGAAC CTGGCCCTGA CCGAGTATAC CTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GAGATGCTCA GCAGAGTTCT AGCCCTGCTG CTGACAATCT CCGTGAGCAG 
TTCGAGCGCC TGATGACCAT CTATCTCTCT ACCAAGACTG CAATGACTGA ACCCCAGATG 
CTGCAGAACT GCCTGAATTT GCAGGTGTCC ATGGCTGTTC TGCTGGTTCA ACTGGCCATA 
GGCAATGAGG GCTCTCAGCC AATAGAGCTA AGTTTTCCCC TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACCTAGGTG ATTTCCTCAT TTTTCTCCGG 
CGCTTTGCTG AGGACATTTT GGAGACGTCA GCAGATTCCC TGGAGCATGT CCTTCATTTT 
ATCACCATTT TCACTGGAAG CATAGAAAGA ATGAAGAATC CCCACCTGAG GGCCAAACTA 
GCAGAGGTGT TAGAAGCAGT GATGCCTCAC CTGGACCAGA CCCCAAGCCC CTTGGTGTCC 
AGTGTGTTCC ACCGAAAACG TGTGTTCTGC AACTTTCCAT ACGCTCCCCA ACTTTCTGAA 
GCCTTGATCA AGGTTTTTGT GGACATCGAA TTTACAGGGG ACCCCCACCA ATTTGAACAG 
AAGTTTAATT ACCGCCGCCC TATGTATCCC ATCCTAAGAT ACATGTGGGG GACAGATAGC 
TATCGGGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCTATGAAT 
CCCCCACTGT TCCTCCGATT TCTTAACCTA TTAATGAATG ATGCTATCTT CCTTTTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAGCAGA TTGAGAAAGA CCGAGGAGAA 
TGGGAGAGCC TGACGCCAGA AGCCCGCCGA GAGAAAGAGG CAGGTCTGCA GATGTTTGGG 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TTGGGACCCT TTCCTTCCTG 
ACATCAGAGA TCAAGTCACT GTTCGTGCAC CCGTTCCTGG CCGAGCGCAT CATCTCCATG 
CTCAACTACT TCCTGCAGCA CCTGGTTGGC CCCAAGATGG GTGCTCTCAA AGTCAAGGAC 
TTCAGCGAAT TTGATTTCAA ACCTCAGCAG CTTGTATCAG ACATCTGCAC TATCTACTTA 
AATCTTGGAG ATGAGGAGAA TTTCTGTGCC ACTGTGCCCA AGGACGGACG TTCCTATTCC 
CCTACTCTCT TTGCACAGAC AGTCCGAGTC CTGAAGAAAA TCAATAAGCC CGGGAATATG 
ATCGTGGCTT TCAGTAACTT GGCAGAGAGA ATCAAGTCTC TTGCAGACCT CCAACAACAA 
GAGGAGGAGA CATATGCAGA TGCCTGCGAT GAGTTCCTGG ACCCCATCAT GAGCACACTG 
ATGTCTGACC CTGTGGTGCT TCCGTCTTCC AGAGTCACTG TGGACGGATC CACCATCGCC 
AGGCATTTAC TCAGTGACCA AACAGATCCT TTTAATCGTA GTCCCCTTAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT GAAAGAAAAA ATTCAACGGT GGCTTGCAGA GAGGAAGCAA 
CAAAAGGAGC AGCTTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997493.1
CDS	146..3346
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIHKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVSEISRSFRTHQEMCEQLNINHMIQRIFLITLDNSDPNLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQAIFARLLLQDPGNHLISMTSSATLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALLLDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFLEYIQPKDPSNGQMYQKTLLGVILNISCLLKTPGVVENHGFFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHGILFWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCVLKDLNDEERKIKSVHMRGLDKETCLIPAVQEPVFPQSYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELSFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFAEDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPSPLVSSVFHRKRVFCNFPYAPQLSEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDSYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWESLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLSFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVVLPSSRVTVDGSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Target ORF information:

RefSeq Version	NM_207610.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitination factor E4A (Ube4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207610.1

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA GTTTGCAGCA ATCCACAAAG AGCAGCTGAA ACAGCAGTCT 
GATGAGCTCC CAGCAAGCCC AGATGACTCA GATAATAGTG TGTCGGAGAG CCTGGATGAG 
TTTGACTACT CAGTGTCTGA GATCAGCCGC TCTTTCCGGA CACATCAAGA AATGTGTGAA 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTCA TTACTCTGGA CAACAGTGAT 
CCAAACTTGA AAAGTGGGAA CGGCATCCCC AGTCGGTGCG TGTACTTGGA AGAAATGGCA 
GTGGAGCTGG AAGATCAAGA CTGGCTGGAT ATGAGCAACG TGGAGCAGGC CATCTTTGCT 
CGGTTACTGC TTCAAGATCC AGGAAACCAC TTGATCAGCA TGACTTCCTC TGCAACTTTA 
AACCTCTCTG CTGATCGCGA TGCAGGAGAG CGGCACATCT TCTGTTACCT TTACTCCTGC 
TTCCAAAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAAA ACCTACTCCC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAACACG CGAACAGTTC TCCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GACTTGATGT TGGAAGCTAT TCAGGGAGCC 
CATTTTGAAG ATGTGACTGA GTTTTTAGAA GAGGTCATTG AAGCCTTGCT ATTGGATGAG 
GAGGTTCGAA CATTTCCTGA AGTCATGATT CCAGTGTTTG ATATTTTACT TGGCCGAATA 
AAAGATCTGG AACTCTGCCA GATCCTCCTG TACGCATACC TGGACATCCT TCTCTATTTC 
ACCAGGCAGA AGGATATGGC CAAGGTTTTT TTAGAGTACA TTCAGCCCAA GGACCCTAGC 
AATGGGCAGA TGTATCAGAA GACCTTGCTG GGAGTCATTC TGAATATCTC GTGCTTGTTA 
AAGACTCCGG GTGTGGTAGA GAATCATGGC TTCTTCCTGA ATCCATCCCG TTCCAGTCCT 
CAGGAGATCA AAGTACAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCATGAAAAG 
ATCTACCAGA TGCTGAAGAA TCTGCTCCAG CTCTCTCCAG AAACCAAGCA TGGTATCTTG 
TTCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATCTG GGCCAATCAG 
ATGCCCGAAA TCTTCTTCCA AATGTATGCC TCGGATGCCT TCTTTCTGAA TCTGGGTGCT 
GCTCTCCTGA AGCTGTGCCA GCCATTCTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AACCCCACAT ACTGTGTCCT CAAGGACTTG AATGATGAAG AACGAAAAAT TAAAAGTGTA 
CATATGAGAG GTTTAGACAA GGAGACCTGT CTGATCCCAG CTGTGCAGGA GCCAGTGTTC 
CCCCAGAGCT ACAATCTGGT GACCGAGAAC CTGGCCCTGA CCGAGTATAC CTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GAGATGCTCA GCAGAGTTCT AGCCCTGCTG CTGACAATCT CCGTGAGCAG 
TTCGAGCGCC TGATGACCAT CTATCTCTCT ACCAAGACTG CAATGACTGA ACCCCAGATG 
CTGCAGAACT GCCTGAATTT GCAGGTGTCC ATGGCTGTTC TGCTGGTTCA ACTGGCCATA 
GGCAATGAGG GCTCTCAGCC AATAGAGCTA AGTTTTCCCC TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACCTAGGTG ATTTCCTCAT TTTTCTCCGG 
CGCTTTGCTG AGGACATTTT GGAGACGTCA GCAGATTCCC TGGAGCATGT CCTTCATTTT 
ATCACCATTT TCACTGGAAG CATAGAAAGA ATGAAGAATC CCCACCTGAG GGCCAAACTA 
GCAGAGGTGT TAGAAGCAGT GATGCCTCAC CTGGACCAGA CCCCAAGCCC CTTGGTGTCC 
AGTGTGTTCC ACCGAAAACG TGTGTTCTGC AACTTTCCAT ACGCTCCCCA ACTTTCTGAA 
GCCTTGATCA AGGTTTTTGT GGACATCGAA TTTACAGGGG ACCCCCACCA ATTTGAACAG 
AAGTTTAATT ACCGCCGCCC TATGTATCCC ATCCTAAGAT ACATGTGGGG GACAGATAGC 
TATCGGGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCTATGAAT 
CCCCCACTGT TCCTCCGATT TCTTAACCTA TTAATGAATG ATGCTATCTT CCTTTTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAGCAGA TTGAGAAAGA CCGAGGAGAA 
TGGGAGAGCC TGACGCCAGA AGCCCGCCGA GAGAAAGAGG CAGGTCTGCA GATGTTTGGG 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TTGGGACCCT TTCCTTCCTG 
ACATCAGAGA TCAAGTCACT GTTCGTGCAC CCGTTCCTGG CCGAGCGCAT CATCTCCATG 
CTCAACTACT TCCTGCAGCA CCTGGTTGGC CCCAAGATGG GTGCTCTCAA AGTCAAGGAC 
TTCAGCGAAT TTGATTTCAA ACCTCAGCAG CTTGTATCAG ACATCTGCAC TATCTACTTA 
AATCTTGGAG ATGAGGAGAA TTTCTGTGCC ACTGTGCCCA AGGACGGACG TTCCTATTCC 
CCTACTCTCT TTGCACAGAC AGTCCGAGTC CTGAAGAAAA TCAATAAGCC CGGGAATATG 
ATCGTGGCTT TCAGTAACTT GGCAGAGAGA ATCAAGTCTC TTGCAGACCT CCAACAACAA 
GAGGAGGAGA CATATGCAGA TGCCTGCGAT GAGTTCCTGG ACCCCATCAT GAGCACACTG 
ATGTCTGACC CTGTGGTGCT TCCGTCTTCC AGAGTCACTG TGGACGGATC CACCATCGCC 
AGGCATTTAC TCAGTGACCA AACAGATCCT TTTAATCGTA GTCCCCTTAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT GAAAGAAAAA ATTCAACGGT GGCTTGCAGA GAGGAAGCAA 
CAAAAGGAGC AGCTTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube4a ( XM_006242938.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243000.1 Rattus norvegicus ubiquitination factor E4A (Ube4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31082
Clone ID Related Accession (Same CDS sequence)	XM_006242938.3
Accession Version	XM_006242938.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin conjugation factor E4 A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242938.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA GTTTGCAGCA ATCCACAAAG AGCAGCTGAA ACAGCAGTCT 
GGTAGTGTGT TTGGTTCTGA GGAAGGAGCA TTGTGCAACA GCCTACATGT TCCGGCAGAT 
GAGCTCCCAG CAAGCCCAGA TGACTCAGAT AATAGTGTGT CGGAGAGCCT GGATGAGTTT 
GACTACTCAG TGTCTGAGAT CAGCCGCTCT TTCCGGACAC ATCAAGAAAT GTGTGAACAA 
CTCAACATCA ATCACATGAT CCAAAGGATC TTCCTCATTA CTCTGGACAA CAGTGATCCA 
AACTTGAAAA GTGGGAACGG CATCCCCAGT CGGTGCGTGT ACTTGGAAGA AATGGCAGTG 
GAGCTGGAAG ATCAAGACTG GCTGGATATG AGCAACGTGG AGCAGGCCAT CTTTGCTCGG 
TTACTGCTTC AAGATCCAGG AAACCACTTG ATCAGCATGA CTTCCTCTGC AACTTTAAAC 
CTCTCTGCTG ATCGCGATGC AGGAGAGCGG CACATCTTCT GTTACCTTTA CTCCTGCTTC 
CAAAGAGCCA AGGAAGAGAT TACCAAAGTT CCAGAAAACC TACTCCCCTT TGCAGTGCAG 
TGCAGAAACC TCACTGTGTC CAACACGCGA ACAGTTCTCC TCACCCCAGA GATCTATGTT 
GACCAAAACA TCCATGAGCA ACTGGTAGAC TTGATGTTGG AAGCTATTCA GGGAGCCCAT 
TTTGAAGATG TGACTGAGTT TTTAGAAGAG GTCATTGAAG CCTTGCTATT GGATGAGGAG 
GTTCGAACAT TTCCTGAAGT CATGATTCCA GTGTTTGATA TTTTACTTGG CCGAATAAAA 
GATCTGGAAC TCTGCCAGAT CCTCCTGTAC GCATACCTGG ACATCCTTCT CTATTTCACC 
AGGCAGAAGG ATATGGCCAA GGTTTTTTTA GAGTACATTC AGCCCAAGGA CCCTAGCAAT 
GGGCAGATGT ATCAGAAGAC CTTGCTGGGA GTCATTCTGA ATATCTCGTG CTTGTTAAAG 
ACTCCGGGTG TGGTAGAGAA TCATGGCTTC TTCCTGAATC CATCCCGTTC CAGTCCTCAG 
GAGATCAAAG TACAGGAGGC CAACATCCAT CAGTTCATGG CTCAGTTCCA TGAAAAGATC 
TACCAGATGC TGAAGAATCT GCTCCAGCTC TCTCCAGAAA CCAAGCATGG TATCTTGTTC 
TGGCTTGGAA ACTGTTTGCA TGCAAATGCA GGCCGCACCA AGATCTGGGC CAATCAGATG 
CCCGAAATCT TCTTCCAAAT GTATGCCTCG GATGCCTTCT TTCTGAATCT GGGTGCTGCT 
CTCCTGAAGC TGTGCCAGCC ATTCTGCAAA CCCAGATCCT CTCGGCTCCT CACCTTTAAC 
CCCACATACT GTGTCCTCAA GGACTTGAAT GATGAAGAAC GAAAAATTAA AAGTGTACAT 
ATGAGAGGTT TAGACAAGGA GACCTGTCTG ATCCCAGCTG TGCAGGAGCC AGTGTTCCCC 
CAGAGCTACA ATCTGGTGAC CGAGAACCTG GCCCTGACCG AGTATACCTT GTACTTAGGA 
TTTCACAGGT TGCATGATCA GATGGTAAAA ATCAACCAAA ATCTGCATCG GCTGCAGGTT 
GCCTGGCGAG ATGCTCAGCA GAGTTCTAGC CCTGCTGCTG ACAATCTCCG TGAGCAGTTC 
GAGCGCCTGA TGACCATCTA TCTCTCTACC AAGACTGCAA TGACTGAACC CCAGATGCTG 
CAGAACTGCC TGAATTTGCA GGTGTCCATG GCTGTTCTGC TGGTTCAACT GGCCATAGGC 
AATGAGGGCT CTCAGCCAAT AGAGCTAAGT TTTCCCCTGC CAGATGGCTA CAGCTCTTTG 
GCTTATGTGC CAGAATTTTT TGCAGATAAC CTAGGTGATT TCCTCATTTT TCTCCGGCGC 
TTTGCTGAGG ACATTTTGGA GACGTCAGCA GATTCCCTGG AGCATGTCCT TCATTTTATC 
ACCATTTTCA CTGGAAGCAT AGAAAGAATG AAGAATCCCC ACCTGAGGGC CAAACTAGCA 
GAGGTGTTAG AAGCAGTGAT GCCTCACCTG GACCAGACCC CAAGCCCCTT GGTGTCCAGT 
GTGTTCCACC GAAAACGTGT GTTCTGCAAC TTTCCATACG CTCCCCAACT TTCTGAAGCC 
TTGATCAAGG TTTTTGTGGA CATCGAATTT ACAGGGGACC CCCACCAATT TGAACAGAAG 
TTTAATTACC GCCGCCCTAT GTATCCCATC CTAAGATACA TGTGGGGGAC AGATAGCTAT 
CGGGAGAGCA TTAAGGATTT GGCTGACTAT GCCTCTAAGA ATTTAGAAGC TATGAATCCC 
CCACTGTTCC TCCGATTTCT TAACCTATTA ATGAATGATG CTATCTTCCT TTTGGATGAA 
GCCATACAGT ATTTGAGCAA GATAAAGATT CAGCAGATTG AGAAAGACCG AGGAGAATGG 
GAGAGCCTGA CGCCAGAAGC CCGCCGAGAG AAAGAGGCAG GTCTGCAGAT GTTTGGGCAG 
CTGGCACGTT TCCATAACAT CATGTCCAAT GAAACAATTG GGACCCTTTC CTTCCTGACA 
TCAGAGATCA AGTCACTGTT CGTGCACCCG TTCCTGGCCG AGCGCATCAT CTCCATGCTC 
AACTACTTCC TGCAGCACCT GGTTGGCCCC AAGATGGGTG CTCTCAAAGT CAAGGACTTC 
AGCGAATTTG ATTTCAAACC TCAGCAGCTT GTATCAGACA TCTGCACTAT CTACTTAAAT 
CTTGGAGATG AGGAGAATTT CTGTGCCACT GTGCCCAAGG ACGGACGTTC CTATTCCCCT 
ACTCTCTTTG CACAGACAGT CCGAGTCCTG AAGAAAATCA ATAAGCCCGG GAATATGATC 
GTGGCTTTCA GTAACTTGGC AGAGAGAATC AAGTCTCTTG CAGACCTCCA ACAACAAGAG 
GAGGAGACAT ATGCAGATGC CTGCGATGAG TTCCTGGACC CCATCATGAG CACACTGATG 
TCTGACCCTG TGGTGCTTCC GTCTTCCAGA GTCACTGTGG ACAGATCCAC CATCGCCAGG 
CATTTACTCA GTGACCAAAC AGATCCTTTT AATCGTAGTC CCCTTACCAT GGACCAGATC 
CGGCCAAACA CAGAACTGAA AGAAAAAATT CAACGGTGGC TTGCAGAGAG GAAGCAACAA 
AAGGAGCAGC TTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243000.1
CDS	301..3558
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIHKEQLKQQSGSVFGSEEGALCNSLHVPADELPASPDDSDNSVSESLDEFDYSVSEISRSFRTHQEMCEQLNINHMIQRIFLITLDNSDPNLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQAIFARLLLQDPGNHLISMTSSATLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALLLDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFLEYIQPKDPSNGQMYQKTLLGVILNISCLLKTPGVVENHGFFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHGILFWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCVLKDLNDEERKIKSVHMRGLDKETCLIPAVQEPVFPQSYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELSFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFAEDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPSPLVSSVFHRKRVFCNFPYAPQLSEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDSYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWESLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLSFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Target ORF information:

RefSeq Version	XM_006242938.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitination factor E4A (Ube4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242938.3

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA GTTTGCAGCA ATCCACAAAG AGCAGCTGAA ACAGCAGTCT 
GGTAGTGTGT TTGGTTCTGA GGAAGGAGCA TTGTGCAACA GCCTACATGT TCCGGCAGAT 
GAGCTCCCAG CAAGCCCAGA TGACTCAGAT AATAGTGTGT CGGAGAGCCT GGATGAGTTT 
GACTACTCAG TGTCTGAGAT CAGCCGCTCT TTCCGGACAC ATCAAGAAAT GTGTGAACAA 
CTCAACATCA ATCACATGAT CCAAAGGATC TTCCTCATTA CTCTGGACAA CAGTGATCCA 
AACTTGAAAA GTGGGAACGG CATCCCCAGT CGGTGCGTGT ACTTGGAAGA AATGGCAGTG 
GAGCTGGAAG ATCAAGACTG GCTGGATATG AGCAACGTGG AGCAGGCCAT CTTTGCTCGG 
TTACTGCTTC AAGATCCAGG AAACCACTTG ATCAGCATGA CTTCCTCTGC AACTTTAAAC 
CTCTCTGCTG ATCGCGATGC AGGAGAGCGG CACATCTTCT GTTACCTTTA CTCCTGCTTC 
CAAAGAGCCA AGGAAGAGAT TACCAAAGTT CCAGAAAACC TACTCCCCTT TGCAGTGCAG 
TGCAGAAACC TCACTGTGTC CAACACGCGA ACAGTTCTCC TCACCCCAGA GATCTATGTT 
GACCAAAACA TCCATGAGCA ACTGGTAGAC TTGATGTTGG AAGCTATTCA GGGAGCCCAT 
TTTGAAGATG TGACTGAGTT TTTAGAAGAG GTCATTGAAG CCTTGCTATT GGATGAGGAG 
GTTCGAACAT TTCCTGAAGT CATGATTCCA GTGTTTGATA TTTTACTTGG CCGAATAAAA 
GATCTGGAAC TCTGCCAGAT CCTCCTGTAC GCATACCTGG ACATCCTTCT CTATTTCACC 
AGGCAGAAGG ATATGGCCAA GGTTTTTTTA GAGTACATTC AGCCCAAGGA CCCTAGCAAT 
GGGCAGATGT ATCAGAAGAC CTTGCTGGGA GTCATTCTGA ATATCTCGTG CTTGTTAAAG 
ACTCCGGGTG TGGTAGAGAA TCATGGCTTC TTCCTGAATC CATCCCGTTC CAGTCCTCAG 
GAGATCAAAG TACAGGAGGC CAACATCCAT CAGTTCATGG CTCAGTTCCA TGAAAAGATC 
TACCAGATGC TGAAGAATCT GCTCCAGCTC TCTCCAGAAA CCAAGCATGG TATCTTGTTC 
TGGCTTGGAA ACTGTTTGCA TGCAAATGCA GGCCGCACCA AGATCTGGGC CAATCAGATG 
CCCGAAATCT TCTTCCAAAT GTATGCCTCG GATGCCTTCT TTCTGAATCT GGGTGCTGCT 
CTCCTGAAGC TGTGCCAGCC ATTCTGCAAA CCCAGATCCT CTCGGCTCCT CACCTTTAAC 
CCCACATACT GTGTCCTCAA GGACTTGAAT GATGAAGAAC GAAAAATTAA AAGTGTACAT 
ATGAGAGGTT TAGACAAGGA GACCTGTCTG ATCCCAGCTG TGCAGGAGCC AGTGTTCCCC 
CAGAGCTACA ATCTGGTGAC CGAGAACCTG GCCCTGACCG AGTATACCTT GTACTTAGGA 
TTTCACAGGT TGCATGATCA GATGGTAAAA ATCAACCAAA ATCTGCATCG GCTGCAGGTT 
GCCTGGCGAG ATGCTCAGCA GAGTTCTAGC CCTGCTGCTG ACAATCTCCG TGAGCAGTTC 
GAGCGCCTGA TGACCATCTA TCTCTCTACC AAGACTGCAA TGACTGAACC CCAGATGCTG 
CAGAACTGCC TGAATTTGCA GGTGTCCATG GCTGTTCTGC TGGTTCAACT GGCCATAGGC 
AATGAGGGCT CTCAGCCAAT AGAGCTAAGT TTTCCCCTGC CAGATGGCTA CAGCTCTTTG 
GCTTATGTGC CAGAATTTTT TGCAGATAAC CTAGGTGATT TCCTCATTTT TCTCCGGCGC 
TTTGCTGAGG ACATTTTGGA GACGTCAGCA GATTCCCTGG AGCATGTCCT TCATTTTATC 
ACCATTTTCA CTGGAAGCAT AGAAAGAATG AAGAATCCCC ACCTGAGGGC CAAACTAGCA 
GAGGTGTTAG AAGCAGTGAT GCCTCACCTG GACCAGACCC CAAGCCCCTT GGTGTCCAGT 
GTGTTCCACC GAAAACGTGT GTTCTGCAAC TTTCCATACG CTCCCCAACT TTCTGAAGCC 
TTGATCAAGG TTTTTGTGGA CATCGAATTT ACAGGGGACC CCCACCAATT TGAACAGAAG 
TTTAATTACC GCCGCCCTAT GTATCCCATC CTAAGATACA TGTGGGGGAC AGATAGCTAT 
CGGGAGAGCA TTAAGGATTT GGCTGACTAT GCCTCTAAGA ATTTAGAAGC TATGAATCCC 
CCACTGTTCC TCCGATTTCT TAACCTATTA ATGAATGATG CTATCTTCCT TTTGGATGAA 
GCCATACAGT ATTTGAGCAA GATAAAGATT CAGCAGATTG AGAAAGACCG AGGAGAATGG 
GAGAGCCTGA CGCCAGAAGC CCGCCGAGAG AAAGAGGCAG GTCTGCAGAT GTTTGGGCAG 
CTGGCACGTT TCCATAACAT CATGTCCAAT GAAACAATTG GGACCCTTTC CTTCCTGACA 
TCAGAGATCA AGTCACTGTT CGTGCACCCG TTCCTGGCCG AGCGCATCAT CTCCATGCTC 
AACTACTTCC TGCAGCACCT GGTTGGCCCC AAGATGGGTG CTCTCAAAGT CAAGGACTTC 
AGCGAATTTG ATTTCAAACC TCAGCAGCTT GTATCAGACA TCTGCACTAT CTACTTAAAT 
CTTGGAGATG AGGAGAATTT CTGTGCCACT GTGCCCAAGG ACGGACGTTC CTATTCCCCT 
ACTCTCTTTG CACAGACAGT CCGAGTCCTG AAGAAAATCA ATAAGCCCGG GAATATGATC 
GTGGCTTTCA GTAACTTGGC AGAGAGAATC AAGTCTCTTG CAGACCTCCA ACAACAAGAG 
GAGGAGACAT ATGCAGATGC CTGCGATGAG TTCCTGGACC CCATCATGAG CACACTGATG 
TCTGACCCTG TGGTGCTTCC GTCTTCCAGA GTCACTGTGG ACAGATCCAC CATCGCCAGG 
CATTTACTCA GTGACCAAAC AGATCCTTTT AATCGTAGTC CCCTTACCAT GGACCAGATC 
CGGCCAAACA CAGAACTGAA AGAAAAAATT CAACGGTGGC TTGCAGAGAG GAAGCAACAA 
AAGGAGCAGC TTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression analysis of the gene encoding for the U-box-type ubiquitin ligase UBE4A in human tissues.
Gene32869-74(2004 Mar)
Contino G,Amati F,Pucci S,Pontieri E,Pichiorri F,Novelli A,Botta A,Mango R,Nardone AM,Sangiuolo FC,Citro G,Spagnoli LG,Novelli G

U box proteins as a new family of ubiquitin-protein ligases.
The Journal of biological chemistry276(35)33111-20(2001 Aug)
Hatakeyama S,Yada M,Matsumoto M,Ishida N,Nakayama KI

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Ube4a cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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