Tet1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tet1
Entrez Gene ID	309902
Full Name	tet methylcytosine dioxygenase 1
Synonyms	Cxxc6
General protein information	Preferred Names tet methylcytosine dioxygenase 1 Names methylcytosine dioxygenase TET1 LOW QUALITY PROTEIN: methylcytosine dioxygenase TET1 CXXC finger 6 tet oncogene 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601794.1	XP_017457283.1	LOW QUALITY PROTEIN: methylcytosine dioxygenase TET1
XM_008774952.1	XP_008773174.1	methylcytosine dioxygenase TET1
XM_006223880.2	XP_006223942.1	methylcytosine dioxygenase TET1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tet1	XP_006256460.1
Pan troglodytes (chimpanzee)	TET1	XP_507822.3
Bos taurus (cattle)	TET1	XP_003588047.2
Macaca mulatta (Rhesus monkey)	TET1	XP_002805756.1
Canis lupus familiaris (dog)	TET1	XP_536371.2
Mus musculus (house mouse)	Tet1	NP_001240786.1
Homo sapiens (human)	TET1	NP_085128.2
Gallus gallus (chicken)	TET1	XP_004942068.1

Gene Ontology

The following Tet1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tet1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa21239	XM_006223880.2 Latest version!	Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa21239	XM_008774952.1 Latest version!	Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa39430	XM_017601794.1 Latest version!	Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tet1 ( XM_008774952.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008773174.1 Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.

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CloneID	ORa21239
Clone ID Related Accession (Same CDS sequence)	XM_008774952.1 , XM_006223880.2
Accession Version	XM_008774952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	methylcytosine dioxygenase TET1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000088.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT CCCGCCCCAC AAAGCCTTCC AAATCAGTCA AAACAAAGCT CCAGAAGAAG 
AGCAGCCAGA TGAAGAAGAA GATATCAAAG CAGGCTGTCA AACATGGGGC ATCAGGGAAG 
GCGCTGACCC CTGGAAAACT GAAGCAATTA ATCAGAAGAA GAGATGGTAA GAAGGAAACG 
GAAGTCAAAA CCCCCACACT GCCGCAGAGT CTTCTGACGA GAGCTGGGGC TGCCCGGTTG 
AATCAGGATC GAAACCAAGT TCTTTTTCAG AACCCAGAGT CCTTAACTTG CAATGGGTTT 
ACAATGGCTC TTCGGAGAAC CTCTCTTAGC TGGCGACTCT CCCAACGCCC AGTAGTCACA 
CCCAAACCTA AGAAAGTGCC ACCTTCAAAG AAGCAGTGTG TACATAATGT CCAGGATGAT 
CCAGGGGTAA AGCACTCCGG AAATGATTCA GGCCCCAGCC AAGATGCTGT CATGTCTCCA 
GATACAGAAA ACCTAACTGG TGAACAGAAC ATGTGTTTAG TTGAAGGCGA GAGCCAAGAG 
ATAACCCAGT CTTGCTCTCA AACAGTAGAA GATTCCCAAA GCTGTATTTC TGAATATGGG 
AACCTTGAAG CCGGAATTTC TTGGTCACTG GAAGGGACAC ACAGTGAAGG ACTACTCTCT 
CATCAGACAT CTAATAATGT GAGCAATTCC CATCCGGGGT GTGCTCCATC GCCCCAAAGA 
TCAACTTCTG AAGTTACCTC TGAGAAAAAT GCCAGTGATC AGTTTGTAGA CTTGTGCTCA 
CCAGTAGAGT CTCCCAAGCT GTCTGATCCT TCTCAGAATC CTATGGGAAG CGAGCATAAT 
TGCTTTCCTG ACTCTAGTTT TAGGATCGTT CCTGAATTGG ACCTTAAAAC CTGCATGTCT 
CTTGATGAGT CTGTATATCC CACAGCTCTG ATAAAATTCA TCTTGGCAGG CTCACAGCCG 
GATGTCCTTG ATACTAAGTC CGAAGAAGAA ACCCTTAAAA CTACCCCAGA AAAAGTGGGT 
AGCCACCCAA ATCAAGTTGT GGATGCCACC TCTATCCTAG GACAGGCCTT TAGTACTCTC 
CCGCATCAGT GGGGATTTCC TGGTGCTAAC TTAGTTCATA GTGAGACCCT GGCCAAGGGC 
TCAGTCTCAC CAGAGGATCT TGGTGCTATT ACTATGCTAA ACCAACCAGA AACTCTGGGT 
ATGGATATGG ATGCTCCCCC GGACCTGCCT ATCTTCCTTC CTATGCCTCC AAACACAGTT 
GCTACCTACA GTTGTCTTCT TTCAGGGCCT GAGCCCCACA CCTCTGCTTC CTGTGGACTT 
GAAGTCCAAG GTGCTACCCC GATTTTAACT TTGGACTCAG GACACACTCC CCAACCCCCA 
CAAAACTCTG AGTTAAGTTC AGTACCTCTG GTAATAGCTG CAAACAGTAC ACAAGCTGAG 
AAGCAGTTTG GTGCCAATCT TTTCCCAGCA AACATACAAG GGTTTACAAT TGCCCCTGAG 
AATGAACTCC AGCATGCTCC ATTAGATCTT ACTCAGGGCT CTCATGCTGC TCCCAGCAAA 
TTGGAAGGAG GGATTTCTCA CGTTAGCATG ACCAGCTCAG CTGATGATGT CAAAGCCACA 
ATGATGTCTA TGCCTGTTAC ACGAGCTAGT ACCTCTTCTC TCCCATGTAA ATCTATGCCA 
CCAACTGTGG AGAGGAGGAA GCGCAAGGCC TGTGGGGTCT GTGAGCCGTG CCAGCAGAAG 
GCCAACTGTG GGGAATGCAC CTACTGCAAG AACAGGAAGA ATAGCCACCA GATCTGTAAG 
AAGAGGAAAT GCGAGGTGCT CAAAAAGAAG CCAGAGGCCA CCTCACGGGT ACAGGTTACA 
AAAGAAAACA AGAGGCCCCA GAGGGAAAAG AAGCCCAAAG TTTTAAAGAC AGACTTTAAC 
AACAAACCAG TAAATGGCCC CAAATCAGAG TCCATGGACT ACAGTAGACG TGGTCATGGG 
GAAGAAGAGC AAAGGTTGGG TTTGTTCACA CATCCCCTTG AAAATGTAAG GAAGAATGCA 
GGAGGCATGA CGGGAATTGA AGTGGAGAAG TGGACACCGA ACAAGAAATC ACACTTAGCA 
GCTGGTCAAG TCAAAGGAAG CCGAGGTGCA AATTTAGCTG GAGTTGAAAA TTCTCAACCC 
TCTGAAGATG ACAAGCAACA AACCAACCCT TCCCCGACAT TCGCACAGAC TGTAAGAAAC 
GGCATGAAAA ATGTACACTG TTTGCCCACT GACACAGACC TTCCACTCAA CAAATTGAGT 
CATGAGGAAT TTAGCAAGGT ATTGGGAAAT AATTCCTCTA AATTGCTGAC TGATCCCTCA 
GATTGCAAGG ATGCCATGAG TGACACTACC TCTAGGGAGG GGTGTGATCA TCTCAAGGGG 
CCGAGGAACA TCGTGCTCTT CCAGAAGCCT GGCTTAAACT GCAGATCTGG TGCAGAATCC 
ACCATCTTTA ATAATCACCC TGATACACAC AGTACTGGTA GCCAGCATCA ACCCCCTGAG 
AAGGTACCCA ACAAAGAACC CAAAGATGGC TCTCCAGTTC AGCCAAGTCT TTTATCCCTA 
ATGAAAGACA GGAGATTAAC GCTGGAACAA GTAGTAGCCA TAGAAGCACT GACTCAGCTC 
TCAGAAGCTC CCTCAGAGAA CTCCTCCCCA TCCAAGACAG AGAAGGATGA AGAAACTGAT 
CAGAGAACAG CTAGCCTACT TAATAGCTGC AAGGATGTCC TTCATTCAGT GAGAAAAGAC 
TTCCAAGACC CGAATGTTCA AGGAAAGAGT CTTCACCATG ACACTGTTGT CTTTAATGGC 
CAGAACAGAG CGTTCAAGTC ACCAGATAGT TTTGCTACTA ACCAAGCACT TATAAAGTCA 
CATGAATATT CAAGGTCACC TACAGCTGGT AAAAGCATTG CTAGTGGGAA AGCTCCCCTT 
GATAGTTCTA AGAATTCATG TCCATTACCA GAAGAAAGTC AAAATCTTGA AAACTTCAGT 
CAGGTACTAA TCTGTGATCA GAATTTGAAT TCCCATGATC CATCATGCCA GGATGCACCC 
TATAGTCAAA TTGAAGAAGA TGTCGCAGCA CAATTAACCC AACTTGCATC CACCATTAAT 
CACATCAACC CAGAGTTCAG GAATGCTGAA AGTACACCAG AAAGCCTTGT TGCAAAGAAC 
ACACAGCAAA AACATAGTCA GGAGAAACGC ATGGTACAAC AGAAACCACC TTCAAGTATA 
CAAAATAAAC CTATTGTACC ATCAACAAAG CCAAAGAAGA CACAGAAAAA GGCAAGACCA 
ACTCCATACA CAAGCAAGCG GAAAAAGAAA GCCCCAGCCC ACAGCTCTCA AGAAAGTGAT 
CAGAAGAAGC AAGAGCGGCT AGCTATAGAG TATAGCAAGA TGCATGACAT TTGGATATCA 
TCCCAGTTTC AGAGGTTTGG CCAGTCTGGT CCCCGTAGTC TTCCTGTTCT GTTGAGAAAT 
ATTCCCATCT TTGACCAAAT ATTAAAGCCT GTGACTCAGA GTAAAACATC CTCACACCCC 
AATAAGTTAT TTCCTCCTAT CAATCAGATA AAATTCACAA GAAACCCTGA ATTGGCAAAA 
GAGACAGTGA AGGTTGAACC ATCAGATTCT CTGCCAACAT GCCAATTCAA GATGGAATCC 
AGTGGGCAGG CATTTTCAGA GCCGGCAGAT AATTCTCAGG GACAGCCCAT GGTAAATGTC 
GATCAGAAAG CTCACCCTCT GCCCCAGTCC CCTCCATCTA ACCAGTGTGC TAATATAATG 
GCTGGTGCTG CCCAAACACA TTTTCATCTA GGCGCTCGAA AGAACCTGGT GCATCAGATT 
CCACCACCGA CGTTGCCTGG TATCTCTCCT GACCCACTCT TACCAGACCC AGCATCAGCC 
CTCAGGAAAG GGAACGTGTT AAGTTCCAAT GGAATTACAG TTGTCACCAC AAAGCATGAA 
GAAGCTCAAA CATCAAGCAA TGGCCCATCG GATCCCACCA CAGACAGTGC ACAGGCAGAT 
TTTAATGGGA ACGTCATGGA CTTCCTTAGC ATGCCTGCAA AAAAACTGAT AGCTGAATTG 
AAGGAACAGG AAGCTGCAAC CTGTCAATGT GCTCGTCCTG ATGGAGGCCC ACAAAAAGAC 
AAAGGCCCGT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTATGGCCA GAAGGGAAAA GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT TAGAAGAAGT 
GGTCCTGAAG AGAAGGTTAT TTGTTTGGTT CGTGAGCGTG TGGACCATTA CTGTTCAACG 
GCTGTGATAG TTGTTCTTAT CCTCCTGTGG GAAGGTATCC CTCGACTAAT GGCTGACCGC 
CTGTACAAGG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC TGACCGAAGA 
TGTACCCTCA ACAAAAAACG TACCTGCACC TGTCAAGGCA CCAACCCAAA AACATGCGGA 
GCGTCTTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGA 
AGTGCAAACC CCAGAAAATT CAGACTTGCT CCAAACTATC CCTTACATGA CTACTATAAG 
AGAATTACTG GAAGGTGTTC TGAAGGAAGT GATGTGAAAA CTGGATGGAT CATTCCAGAA 
CGAAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAGG AGAATTTACA AGACTTGGCT 
ACAGTATTAG CTCCAGTTTA TAAGCAGATG GCGCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGACA TTGCTGGAGA CTGTCGACTT GGTAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT CTGTGCTCAT TCTCACAAGG ACATTCACAA CATGAACAAT 
GGAAGCACTG TGGTTTGTAC ATTGATTCGA GAAGATGGCC GTGACAGAAG TGTTCCTGGG 
GATGAGCAGC TACACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAAGCCAA GATCCAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TGTGTTTCAG TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAGATGAAG 
GAGAAGGACA AGAAATCAGG TGCTACTGGC CTCAGCAAGA AGAGGAACTC TGCCTGCACA 
AAGGGAGCCC CAGGTTCGCA CAACACTAAG ATCTATTCAT CAGCCTCATC TACTCCTCAC 
CCAGTGAAAG ATGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCAC AGAAACATCT 
ACCTGTATGT ACAATAAAAC AGCCTCAGGT GGCTTTCCAG AAACAAGTAG CATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTCCTGGGGA ATGTACTGGA 
ATTGTGCAGA CTGCTGCTGA AGTGGCTGCT CTTCCTCACC AATCTCTTCC CATAGCAGAT 
TCTCCCCTTC ATGCTGAGCC TCTCACAAGT CCACCTGATC ATCTAACTTC TAACCAGTCA 
AACCAGCAGC TCCCTTTCCT CAGCTCTCCT CAGAAACTGG CTTCCTGTCA GGTGGAAGAT 
GAACGGCATT CGGAAACAGA TGAGCCTCCT CAACCCGAGG ATGGTAACAT CCCTCAACTT 
GATGAATTTT GGTCAGACAG TGAGGAGATC TATAATGATC CTGCCTTTGG TGGCGTGGCG 
ATAGCACCCA TTCATGGCTC AGTGCTGATT GAGTGTGCCC GGAAAGAGCT TCATGCTACC 
ACCTCTTTGA GTTCTCCCAA ACGAGAGGTC CCTTTTCGTG TGTCCCTTGT ATTCTACCAG 
CACAAAAATC TAAATAAGCC TAATCACGGT TTTGATATCA ACAGAATTAA GTGTAAATGT 
AAAAAAGTAA CAAAAAAACC CCCCGCAGAC AGGGAGGATC CTGATTTATC TCCTGAAACC 
AATTTATCAT ACCAAATTCC TTCTCGAGTT GCATCAACCT TAACCAGCGA CAACGTTGTT 
ACCGTGTCCC CTTATGCTCT CACTCACGTC GCGGGACCCT ACAACCGTTG GGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008773174.1
CDS	539..6715
Translation	MSRSRPTKPSKSVKTKLQKKSSQMKKKISKQAVKHGASGKALTPGKLKQLIRRRDGKKETEVKTPTLPQSLLTRAGAARLNQDRNQVLFQNPESLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCVHNVQDDPGVKHSGNDSGPSQDAVMSPDTENLTGEQNMCLVEGESQEITQSCSQTVEDSQSCISEYGNLEAGISWSLEGTHSEGLLSHQTSNNVSNSHPGCAPSPQRSTSEVTSEKNASDQFVDLCSPVESPKLSDPSQNPMGSEHNCFPDSSFRIVPELDLKTCMSLDESVYPTALIKFILAGSQPDVLDTKSEEETLKTTPEKVGSHPNQVVDATSILGQAFSTLPHQWGFPGANLVHSETLAKGSVSPEDLGAITMLNQPETLGMDMDAPPDLPIFLPMPPNTVATYSCLLSGPEPHTSASCGLEVQGATPILTLDSGHTPQPPQNSELSSVPLVIAANSTQAEKQFGANLFPANIQGFTIAPENELQHAPLDLTQGSHAAPSKLEGGISHVSMTSSADDVKATMMSMPVTRASTSSLPCKSMPPTVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSRVQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDYSRRGHGEEEQRLGLFTHPLENVRKNAGGMTGIEVEKWTPNKKSHLAAGQVKGSRGANLAGVENSQPSEDDKQQTNPSPTFAQTVRNGMKNVHCLPTDTDLPLNKLSHEEFSKVLGNNSSKLLTDPSDCKDAMSDTTSREGCDHLKGPRNIVLFQKPGLNCRSGAESTIFNNHPDTHSTGSQHQPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSENSSPSKTEKDEETDQRTASLLNSCKDVLHSVRKDFQDPNVQGKSLHHDTVVFNGQNRAFKSPDSFATNQALIKSHEYSRSPTAGKSIASGKAPLDSSKNSCPLPEESQNLENFSQVLICDQNLNSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINPEFRNAESTPESLVAKNTQQKHSQEKRMVQQKPPSSIQNKPIVPSTKPKKTQKKARPTPYTSKRKKKAPAHSSQESDQKKQERLAIEYSKMHDIWISSQFQRFGQSGPRSLPVLLRNIPIFDQILKPVTQSKTSSHPNKLFPPINQIKFTRNPELAKETVKVEPSDSLPTCQFKMESSGQAFSEPADNSQGQPMVNVDQKAHPLPQSPPSNQCANIMAGAAQTHFHLGARKNLVHQIPPPTLPGISPDPLLPDPASALRKGNVLSSNGITVVTTKHEEAQTSSNGPSDPTTDSAQADFNGNVMDFLSMPAKKLIAELKEQEAATCQCARPDGGPQKDKGPYYTHLGAGPSVAAVRELMETRYGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKVICLVRERVDHYCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGTNPKTCGASFSFGCSWSMYFNGCKFGRSANPRKFRLAPNYPLHDYYKRITGRCSEGSDVKTGWIIPERKTLISREEKQLEENLQDLATVLAPVYKQMAPVAYQNQVEYEDIAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMNNGSTVVCTLIREDGRDRSVPGDEQLHVLPLYRLADTDEFGSVEGMKAKIQSGAIQVNGPTRKRRLCFSEPVPRCGKRAKMKEKDKKSGATGLSKKRNSACTKGAPGSHNTKIYSSASSTPHPVKDESTDFCPLQASSTETSTCMYNKTASGGFPETSSILHCTMPSGAHSGANAAPGECTGIVQTAAEVAALPHQSLPIADSPLHAEPLTSPPDHLTSNQSNQQLPFLSSPQKLASCQVEDERHSETDEPPQPEDGNIPQLDEFWSDSEEIYNDPAFGGVAIAPIHGSVLIECARKELHATTSLSSPKREVPFRVSLVFYQHKNLNKPNHGFDINRIKCKCKKVTKKPPADREDPDLSPETNLSYQIPSRVASTLTSDNVVTVSPYALTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_008774952.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008774952.1

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCAC AAAGCCTTCC AAATCAGTCA AAACAAAGCT CCAGAAGAAG 
AGCAGCCAGA TGAAGAAGAA GATATCAAAG CAGGCTGTCA AACATGGGGC ATCAGGGAAG 
GCGCTGACCC CTGGAAAACT GAAGCAATTA ATCAGAAGAA GAGATGGTAA GAAGGAAACG 
GAAGTCAAAA CCCCCACACT GCCGCAGAGT CTTCTGACGA GAGCTGGGGC TGCCCGGTTG 
AATCAGGATC GAAACCAAGT TCTTTTTCAG AACCCAGAGT CCTTAACTTG CAATGGGTTT 
ACAATGGCTC TTCGGAGAAC CTCTCTTAGC TGGCGACTCT CCCAACGCCC AGTAGTCACA 
CCCAAACCTA AGAAAGTGCC ACCTTCAAAG AAGCAGTGTG TACATAATGT CCAGGATGAT 
CCAGGGGTAA AGCACTCCGG AAATGATTCA GGCCCCAGCC AAGATGCTGT CATGTCTCCA 
GATACAGAAA ACCTAACTGG TGAACAGAAC ATGTGTTTAG TTGAAGGCGA GAGCCAAGAG 
ATAACCCAGT CTTGCTCTCA AACAGTAGAA GATTCCCAAA GCTGTATTTC TGAATATGGG 
AACCTTGAAG CCGGAATTTC TTGGTCACTG GAAGGGACAC ACAGTGAAGG ACTACTCTCT 
CATCAGACAT CTAATAATGT GAGCAATTCC CATCCGGGGT GTGCTCCATC GCCCCAAAGA 
TCAACTTCTG AAGTTACCTC TGAGAAAAAT GCCAGTGATC AGTTTGTAGA CTTGTGCTCA 
CCAGTAGAGT CTCCCAAGCT GTCTGATCCT TCTCAGAATC CTATGGGAAG CGAGCATAAT 
TGCTTTCCTG ACTCTAGTTT TAGGATCGTT CCTGAATTGG ACCTTAAAAC CTGCATGTCT 
CTTGATGAGT CTGTATATCC CACAGCTCTG ATAAAATTCA TCTTGGCAGG CTCACAGCCG 
GATGTCCTTG ATACTAAGTC CGAAGAAGAA ACCCTTAAAA CTACCCCAGA AAAAGTGGGT 
AGCCACCCAA ATCAAGTTGT GGATGCCACC TCTATCCTAG GACAGGCCTT TAGTACTCTC 
CCGCATCAGT GGGGATTTCC TGGTGCTAAC TTAGTTCATA GTGAGACCCT GGCCAAGGGC 
TCAGTCTCAC CAGAGGATCT TGGTGCTATT ACTATGCTAA ACCAACCAGA AACTCTGGGT 
ATGGATATGG ATGCTCCCCC GGACCTGCCT ATCTTCCTTC CTATGCCTCC AAACACAGTT 
GCTACCTACA GTTGTCTTCT TTCAGGGCCT GAGCCCCACA CCTCTGCTTC CTGTGGACTT 
GAAGTCCAAG GTGCTACCCC GATTTTAACT TTGGACTCAG GACACACTCC CCAACCCCCA 
CAAAACTCTG AGTTAAGTTC AGTACCTCTG GTAATAGCTG CAAACAGTAC ACAAGCTGAG 
AAGCAGTTTG GTGCCAATCT TTTCCCAGCA AACATACAAG GGTTTACAAT TGCCCCTGAG 
AATGAACTCC AGCATGCTCC ATTAGATCTT ACTCAGGGCT CTCATGCTGC TCCCAGCAAA 
TTGGAAGGAG GGATTTCTCA CGTTAGCATG ACCAGCTCAG CTGATGATGT CAAAGCCACA 
ATGATGTCTA TGCCTGTTAC ACGAGCTAGT ACCTCTTCTC TCCCATGTAA ATCTATGCCA 
CCAACTGTGG AGAGGAGGAA GCGCAAGGCC TGTGGGGTCT GTGAGCCGTG CCAGCAGAAG 
GCCAACTGTG GGGAATGCAC CTACTGCAAG AACAGGAAGA ATAGCCACCA GATCTGTAAG 
AAGAGGAAAT GCGAGGTGCT CAAAAAGAAG CCAGAGGCCA CCTCACGGGT ACAGGTTACA 
AAAGAAAACA AGAGGCCCCA GAGGGAAAAG AAGCCCAAAG TTTTAAAGAC AGACTTTAAC 
AACAAACCAG TAAATGGCCC CAAATCAGAG TCCATGGACT ACAGTAGACG TGGTCATGGG 
GAAGAAGAGC AAAGGTTGGG TTTGTTCACA CATCCCCTTG AAAATGTAAG GAAGAATGCA 
GGAGGCATGA CGGGAATTGA AGTGGAGAAG TGGACACCGA ACAAGAAATC ACACTTAGCA 
GCTGGTCAAG TCAAAGGAAG CCGAGGTGCA AATTTAGCTG GAGTTGAAAA TTCTCAACCC 
TCTGAAGATG ACAAGCAACA AACCAACCCT TCCCCGACAT TCGCACAGAC TGTAAGAAAC 
GGCATGAAAA ATGTACACTG TTTGCCCACT GACACAGACC TTCCACTCAA CAAATTGAGT 
CATGAGGAAT TTAGCAAGGT ATTGGGAAAT AATTCCTCTA AATTGCTGAC TGATCCCTCA 
GATTGCAAGG ATGCCATGAG TGACACTACC TCTAGGGAGG GGTGTGATCA TCTCAAGGGG 
CCGAGGAACA TCGTGCTCTT CCAGAAGCCT GGCTTAAACT GCAGATCTGG TGCAGAATCC 
ACCATCTTTA ATAATCACCC TGATACACAC AGTACTGGTA GCCAGCATCA ACCCCCTGAG 
AAGGTACCCA ACAAAGAACC CAAAGATGGC TCTCCAGTTC AGCCAAGTCT TTTATCCCTA 
ATGAAAGACA GGAGATTAAC GCTGGAACAA GTAGTAGCCA TAGAAGCACT GACTCAGCTC 
TCAGAAGCTC CCTCAGAGAA CTCCTCCCCA TCCAAGACAG AGAAGGATGA AGAAACTGAT 
CAGAGAACAG CTAGCCTACT TAATAGCTGC AAGGATGTCC TTCATTCAGT GAGAAAAGAC 
TTCCAAGACC CGAATGTTCA AGGAAAGAGT CTTCACCATG ACACTGTTGT CTTTAATGGC 
CAGAACAGAG CGTTCAAGTC ACCAGATAGT TTTGCTACTA ACCAAGCACT TATAAAGTCA 
CATGAATATT CAAGGTCACC TACAGCTGGT AAAAGCATTG CTAGTGGGAA AGCTCCCCTT 
GATAGTTCTA AGAATTCATG TCCATTACCA GAAGAAAGTC AAAATCTTGA AAACTTCAGT 
CAGGTACTAA TCTGTGATCA GAATTTGAAT TCCCATGATC CATCATGCCA GGATGCACCC 
TATAGTCAAA TTGAAGAAGA TGTCGCAGCA CAATTAACCC AACTTGCATC CACCATTAAT 
CACATCAACC CAGAGTTCAG GAATGCTGAA AGTACACCAG AAAGCCTTGT TGCAAAGAAC 
ACACAGCAAA AACATAGTCA GGAGAAACGC ATGGTACAAC AGAAACCACC TTCAAGTATA 
CAAAATAAAC CTATTGTACC ATCAACAAAG CCAAAGAAGA CACAGAAAAA GGCAAGACCA 
ACTCCATACA CAAGCAAGCG GAAAAAGAAA GCCCCAGCCC ACAGCTCTCA AGAAAGTGAT 
CAGAAGAAGC AAGAGCGGCT AGCTATAGAG TATAGCAAGA TGCATGACAT TTGGATATCA 
TCCCAGTTTC AGAGGTTTGG CCAGTCTGGT CCCCGTAGTC TTCCTGTTCT GTTGAGAAAT 
ATTCCCATCT TTGACCAAAT ATTAAAGCCT GTGACTCAGA GTAAAACATC CTCACACCCC 
AATAAGTTAT TTCCTCCTAT CAATCAGATA AAATTCACAA GAAACCCTGA ATTGGCAAAA 
GAGACAGTGA AGGTTGAACC ATCAGATTCT CTGCCAACAT GCCAATTCAA GATGGAATCC 
AGTGGGCAGG CATTTTCAGA GCCGGCAGAT AATTCTCAGG GACAGCCCAT GGTAAATGTC 
GATCAGAAAG CTCACCCTCT GCCCCAGTCC CCTCCATCTA ACCAGTGTGC TAATATAATG 
GCTGGTGCTG CCCAAACACA TTTTCATCTA GGCGCTCGAA AGAACCTGGT GCATCAGATT 
CCACCACCGA CGTTGCCTGG TATCTCTCCT GACCCACTCT TACCAGACCC AGCATCAGCC 
CTCAGGAAAG GGAACGTGTT AAGTTCCAAT GGAATTACAG TTGTCACCAC AAAGCATGAA 
GAAGCTCAAA CATCAAGCAA TGGCCCATCG GATCCCACCA CAGACAGTGC ACAGGCAGAT 
TTTAATGGGA ACGTCATGGA CTTCCTTAGC ATGCCTGCAA AAAAACTGAT AGCTGAATTG 
AAGGAACAGG AAGCTGCAAC CTGTCAATGT GCTCGTCCTG ATGGAGGCCC ACAAAAAGAC 
AAAGGCCCGT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTATGGCCA GAAGGGAAAA GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT TAGAAGAAGT 
GGTCCTGAAG AGAAGGTTAT TTGTTTGGTT CGTGAGCGTG TGGACCATTA CTGTTCAACG 
GCTGTGATAG TTGTTCTTAT CCTCCTGTGG GAAGGTATCC CTCGACTAAT GGCTGACCGC 
CTGTACAAGG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC TGACCGAAGA 
TGTACCCTCA ACAAAAAACG TACCTGCACC TGTCAAGGCA CCAACCCAAA AACATGCGGA 
GCGTCTTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGA 
AGTGCAAACC CCAGAAAATT CAGACTTGCT CCAAACTATC CCTTACATGA CTACTATAAG 
AGAATTACTG GAAGGTGTTC TGAAGGAAGT GATGTGAAAA CTGGATGGAT CATTCCAGAA 
CGAAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAGG AGAATTTACA AGACTTGGCT 
ACAGTATTAG CTCCAGTTTA TAAGCAGATG GCGCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGACA TTGCTGGAGA CTGTCGACTT GGTAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT CTGTGCTCAT TCTCACAAGG ACATTCACAA CATGAACAAT 
GGAAGCACTG TGGTTTGTAC ATTGATTCGA GAAGATGGCC GTGACAGAAG TGTTCCTGGG 
GATGAGCAGC TACACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAAGCCAA GATCCAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TGTGTTTCAG TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAGATGAAG 
GAGAAGGACA AGAAATCAGG TGCTACTGGC CTCAGCAAGA AGAGGAACTC TGCCTGCACA 
AAGGGAGCCC CAGGTTCGCA CAACACTAAG ATCTATTCAT CAGCCTCATC TACTCCTCAC 
CCAGTGAAAG ATGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCAC AGAAACATCT 
ACCTGTATGT ACAATAAAAC AGCCTCAGGT GGCTTTCCAG AAACAAGTAG CATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTCCTGGGGA ATGTACTGGA 
ATTGTGCAGA CTGCTGCTGA AGTGGCTGCT CTTCCTCACC AATCTCTTCC CATAGCAGAT 
TCTCCCCTTC ATGCTGAGCC TCTCACAAGT CCACCTGATC ATCTAACTTC TAACCAGTCA 
AACCAGCAGC TCCCTTTCCT CAGCTCTCCT CAGAAACTGG CTTCCTGTCA GGTGGAAGAT 
GAACGGCATT CGGAAACAGA TGAGCCTCCT CAACCCGAGG ATGGTAACAT CCCTCAACTT 
GATGAATTTT GGTCAGACAG TGAGGAGATC TATAATGATC CTGCCTTTGG TGGCGTGGCG 
ATAGCACCCA TTCATGGCTC AGTGCTGATT GAGTGTGCCC GGAAAGAGCT TCATGCTACC 
ACCTCTTTGA GTTCTCCCAA ACGAGAGGTC CCTTTTCGTG TGTCCCTTGT ATTCTACCAG 
CACAAAAATC TAAATAAGCC TAATCACGGT TTTGATATCA ACAGAATTAA GTGTAAATGT 
AAAAAAGTAA CAAAAAAACC CCCCGCAGAC AGGGAGGATC CTGATTTATC TCCTGAAACC 
AATTTATCAT ACCAAATTCC TTCTCGAGTT GCATCAACCT TAACCAGCGA CAACGTTGTT 
ACCGTGTCCC CTTATGCTCT CACTCACGTC GCGGGACCCT ACAACCGTTG GGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_006223880.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006223942.1 Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.

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CloneID	ORa21239
Clone ID Related Accession (Same CDS sequence)	XM_008774952.1 , XM_006223880.2
Accession Version	XM_006223880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	methylcytosine dioxygenase TET1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000088.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006223880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT CCCGCCCCAC AAAGCCTTCC AAATCAGTCA AAACAAAGCT CCAGAAGAAG 
AGCAGCCAGA TGAAGAAGAA GATATCAAAG CAGGCTGTCA AACATGGGGC ATCAGGGAAG 
GCGCTGACCC CTGGAAAACT GAAGCAATTA ATCAGAAGAA GAGATGGTAA GAAGGAAACG 
GAAGTCAAAA CCCCCACACT GCCGCAGAGT CTTCTGACGA GAGCTGGGGC TGCCCGGTTG 
AATCAGGATC GAAACCAAGT TCTTTTTCAG AACCCAGAGT CCTTAACTTG CAATGGGTTT 
ACAATGGCTC TTCGGAGAAC CTCTCTTAGC TGGCGACTCT CCCAACGCCC AGTAGTCACA 
CCCAAACCTA AGAAAGTGCC ACCTTCAAAG AAGCAGTGTG TACATAATGT CCAGGATGAT 
CCAGGGGTAA AGCACTCCGG AAATGATTCA GGCCCCAGCC AAGATGCTGT CATGTCTCCA 
GATACAGAAA ACCTAACTGG TGAACAGAAC ATGTGTTTAG TTGAAGGCGA GAGCCAAGAG 
ATAACCCAGT CTTGCTCTCA AACAGTAGAA GATTCCCAAA GCTGTATTTC TGAATATGGG 
AACCTTGAAG CCGGAATTTC TTGGTCACTG GAAGGGACAC ACAGTGAAGG ACTACTCTCT 
CATCAGACAT CTAATAATGT GAGCAATTCC CATCCGGGGT GTGCTCCATC GCCCCAAAGA 
TCAACTTCTG AAGTTACCTC TGAGAAAAAT GCCAGTGATC AGTTTGTAGA CTTGTGCTCA 
CCAGTAGAGT CTCCCAAGCT GTCTGATCCT TCTCAGAATC CTATGGGAAG CGAGCATAAT 
TGCTTTCCTG ACTCTAGTTT TAGGATCGTT CCTGAATTGG ACCTTAAAAC CTGCATGTCT 
CTTGATGAGT CTGTATATCC CACAGCTCTG ATAAAATTCA TCTTGGCAGG CTCACAGCCG 
GATGTCCTTG ATACTAAGTC CGAAGAAGAA ACCCTTAAAA CTACCCCAGA AAAAGTGGGT 
AGCCACCCAA ATCAAGTTGT GGATGCCACC TCTATCCTAG GACAGGCCTT TAGTACTCTC 
CCGCATCAGT GGGGATTTCC TGGTGCTAAC TTAGTTCATA GTGAGACCCT GGCCAAGGGC 
TCAGTCTCAC CAGAGGATCT TGGTGCTATT ACTATGCTAA ACCAACCAGA AACTCTGGGT 
ATGGATATGG ATGCTCCCCC GGACCTGCCT ATCTTCCTTC CTATGCCTCC AAACACAGTT 
GCTACCTACA GTTGTCTTCT TTCAGGGCCT GAGCCCCACA CCTCTGCTTC CTGTGGACTT 
GAAGTCCAAG GTGCTACCCC GATTTTAACT TTGGACTCAG GACACACTCC CCAACCCCCA 
CAAAACTCTG AGTTAAGTTC AGTACCTCTG GTAATAGCTG CAAACAGTAC ACAAGCTGAG 
AAGCAGTTTG GTGCCAATCT TTTCCCAGCA AACATACAAG GGTTTACAAT TGCCCCTGAG 
AATGAACTCC AGCATGCTCC ATTAGATCTT ACTCAGGGCT CTCATGCTGC TCCCAGCAAA 
TTGGAAGGAG GGATTTCTCA CGTTAGCATG ACCAGCTCAG CTGATGATGT CAAAGCCACA 
ATGATGTCTA TGCCTGTTAC ACGAGCTAGT ACCTCTTCTC TCCCATGTAA ATCTATGCCA 
CCAACTGTGG AGAGGAGGAA GCGCAAGGCC TGTGGGGTCT GTGAGCCGTG CCAGCAGAAG 
GCCAACTGTG GGGAATGCAC CTACTGCAAG AACAGGAAGA ATAGCCACCA GATCTGTAAG 
AAGAGGAAAT GCGAGGTGCT CAAAAAGAAG CCAGAGGCCA CCTCACGGGT ACAGGTTACA 
AAAGAAAACA AGAGGCCCCA GAGGGAAAAG AAGCCCAAAG TTTTAAAGAC AGACTTTAAC 
AACAAACCAG TAAATGGCCC CAAATCAGAG TCCATGGACT ACAGTAGACG TGGTCATGGG 
GAAGAAGAGC AAAGGTTGGG TTTGTTCACA CATCCCCTTG AAAATGTAAG GAAGAATGCA 
GGAGGCATGA CGGGAATTGA AGTGGAGAAG TGGACACCGA ACAAGAAATC ACACTTAGCA 
GCTGGTCAAG TCAAAGGAAG CCGAGGTGCA AATTTAGCTG GAGTTGAAAA TTCTCAACCC 
TCTGAAGATG ACAAGCAACA AACCAACCCT TCCCCGACAT TCGCACAGAC TGTAAGAAAC 
GGCATGAAAA ATGTACACTG TTTGCCCACT GACACAGACC TTCCACTCAA CAAATTGAGT 
CATGAGGAAT TTAGCAAGGT ATTGGGAAAT AATTCCTCTA AATTGCTGAC TGATCCCTCA 
GATTGCAAGG ATGCCATGAG TGACACTACC TCTAGGGAGG GGTGTGATCA TCTCAAGGGG 
CCGAGGAACA TCGTGCTCTT CCAGAAGCCT GGCTTAAACT GCAGATCTGG TGCAGAATCC 
ACCATCTTTA ATAATCACCC TGATACACAC AGTACTGGTA GCCAGCATCA ACCCCCTGAG 
AAGGTACCCA ACAAAGAACC CAAAGATGGC TCTCCAGTTC AGCCAAGTCT TTTATCCCTA 
ATGAAAGACA GGAGATTAAC GCTGGAACAA GTAGTAGCCA TAGAAGCACT GACTCAGCTC 
TCAGAAGCTC CCTCAGAGAA CTCCTCCCCA TCCAAGACAG AGAAGGATGA AGAAACTGAT 
CAGAGAACAG CTAGCCTACT TAATAGCTGC AAGGATGTCC TTCATTCAGT GAGAAAAGAC 
TTCCAAGACC CGAATGTTCA AGGAAAGAGT CTTCACCATG ACACTGTTGT CTTTAATGGC 
CAGAACAGAG CGTTCAAGTC ACCAGATAGT TTTGCTACTA ACCAAGCACT TATAAAGTCA 
CATGAATATT CAAGGTCACC TACAGCTGGT AAAAGCATTG CTAGTGGGAA AGCTCCCCTT 
GATAGTTCTA AGAATTCATG TCCATTACCA GAAGAAAGTC AAAATCTTGA AAACTTCAGT 
CAGGTACTAA TCTGTGATCA GAATTTGAAT TCCCATGATC CATCATGCCA GGATGCACCC 
TATAGTCAAA TTGAAGAAGA TGTCGCAGCA CAATTAACCC AACTTGCATC CACCATTAAT 
CACATCAACC CAGAGTTCAG GAATGCTGAA AGTACACCAG AAAGCCTTGT TGCAAAGAAC 
ACACAGCAAA AACATAGTCA GGAGAAACGC ATGGTACAAC AGAAACCACC TTCAAGTATA 
CAAAATAAAC CTATTGTACC ATCAACAAAG CCAAAGAAGA CACAGAAAAA GGCAAGACCA 
ACTCCATACA CAAGCAAGCG GAAAAAGAAA GCCCCAGCCC ACAGCTCTCA AGAAAGTGAT 
CAGAAGAAGC AAGAGCGGCT AGCTATAGAG TATAGCAAGA TGCATGACAT TTGGATATCA 
TCCCAGTTTC AGAGGTTTGG CCAGTCTGGT CCCCGTAGTC TTCCTGTTCT GTTGAGAAAT 
ATTCCCATCT TTGACCAAAT ATTAAAGCCT GTGACTCAGA GTAAAACATC CTCACACCCC 
AATAAGTTAT TTCCTCCTAT CAATCAGATA AAATTCACAA GAAACCCTGA ATTGGCAAAA 
GAGACAGTGA AGGTTGAACC ATCAGATTCT CTGCCAACAT GCCAATTCAA GATGGAATCC 
AGTGGGCAGG CATTTTCAGA GCCGGCAGAT AATTCTCAGG GACAGCCCAT GGTAAATGTC 
GATCAGAAAG CTCACCCTCT GCCCCAGTCC CCTCCATCTA ACCAGTGTGC TAATATAATG 
GCTGGTGCTG CCCAAACACA TTTTCATCTA GGCGCTCGAA AGAACCTGGT GCATCAGATT 
CCACCACCGA CGTTGCCTGG TATCTCTCCT GACCCACTCT TACCAGACCC AGCATCAGCC 
CTCAGGAAAG GGAACGTGTT AAGTTCCAAT GGAATTACAG TTGTCACCAC AAAGCATGAA 
GAAGCTCAAA CATCAAGCAA TGGCCCATCG GATCCCACCA CAGACAGTGC ACAGGCAGAT 
TTTAATGGGA ACGTCATGGA CTTCCTTAGC ATGCCTGCAA AAAAACTGAT AGCTGAATTG 
AAGGAACAGG AAGCTGCAAC CTGTCAATGT GCTCGTCCTG ATGGAGGCCC ACAAAAAGAC 
AAAGGCCCGT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTATGGCCA GAAGGGAAAA GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT TAGAAGAAGT 
GGTCCTGAAG AGAAGGTTAT TTGTTTGGTT CGTGAGCGTG TGGACCATTA CTGTTCAACG 
GCTGTGATAG TTGTTCTTAT CCTCCTGTGG GAAGGTATCC CTCGACTAAT GGCTGACCGC 
CTGTACAAGG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC TGACCGAAGA 
TGTACCCTCA ACAAAAAACG TACCTGCACC TGTCAAGGCA CCAACCCAAA AACATGCGGA 
GCGTCTTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGA 
AGTGCAAACC CCAGAAAATT CAGACTTGCT CCAAACTATC CCTTACATGA CTACTATAAG 
AGAATTACTG GAAGGTGTTC TGAAGGAAGT GATGTGAAAA CTGGATGGAT CATTCCAGAA 
CGAAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAGG AGAATTTACA AGACTTGGCT 
ACAGTATTAG CTCCAGTTTA TAAGCAGATG GCGCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGACA TTGCTGGAGA CTGTCGACTT GGTAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT CTGTGCTCAT TCTCACAAGG ACATTCACAA CATGAACAAT 
GGAAGCACTG TGGTTTGTAC ATTGATTCGA GAAGATGGCC GTGACAGAAG TGTTCCTGGG 
GATGAGCAGC TACACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAAGCCAA GATCCAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TGTGTTTCAG TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAGATGAAG 
GAGAAGGACA AGAAATCAGG TGCTACTGGC CTCAGCAAGA AGAGGAACTC TGCCTGCACA 
AAGGGAGCCC CAGGTTCGCA CAACACTAAG ATCTATTCAT CAGCCTCATC TACTCCTCAC 
CCAGTGAAAG ATGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCAC AGAAACATCT 
ACCTGTATGT ACAATAAAAC AGCCTCAGGT GGCTTTCCAG AAACAAGTAG CATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTCCTGGGGA ATGTACTGGA 
ATTGTGCAGA CTGCTGCTGA AGTGGCTGCT CTTCCTCACC AATCTCTTCC CATAGCAGAT 
TCTCCCCTTC ATGCTGAGCC TCTCACAAGT CCACCTGATC ATCTAACTTC TAACCAGTCA 
AACCAGCAGC TCCCTTTCCT CAGCTCTCCT CAGAAACTGG CTTCCTGTCA GGTGGAAGAT 
GAACGGCATT CGGAAACAGA TGAGCCTCCT CAACCCGAGG ATGGTAACAT CCCTCAACTT 
GATGAATTTT GGTCAGACAG TGAGGAGATC TATAATGATC CTGCCTTTGG TGGCGTGGCG 
ATAGCACCCA TTCATGGCTC AGTGCTGATT GAGTGTGCCC GGAAAGAGCT TCATGCTACC 
ACCTCTTTGA GTTCTCCCAA ACGAGAGGTC CCTTTTCGTG TGTCCCTTGT ATTCTACCAG 
CACAAAAATC TAAATAAGCC TAATCACGGT TTTGATATCA ACAGAATTAA GTGTAAATGT 
AAAAAAGTAA CAAAAAAACC CCCCGCAGAC AGGGAGGATC CTGATTTATC TCCTGAAACC 
AATTTATCAT ACCAAATTCC TTCTCGAGTT GCATCAACCT TAACCAGCGA CAACGTTGTT 
ACCGTGTCCC CTTATGCTCT CACTCACGTC GCGGGACCCT ACAACCGTTG GGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006223942.1
CDS	377..6553
Translation	MSRSRPTKPSKSVKTKLQKKSSQMKKKISKQAVKHGASGKALTPGKLKQLIRRRDGKKETEVKTPTLPQSLLTRAGAARLNQDRNQVLFQNPESLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCVHNVQDDPGVKHSGNDSGPSQDAVMSPDTENLTGEQNMCLVEGESQEITQSCSQTVEDSQSCISEYGNLEAGISWSLEGTHSEGLLSHQTSNNVSNSHPGCAPSPQRSTSEVTSEKNASDQFVDLCSPVESPKLSDPSQNPMGSEHNCFPDSSFRIVPELDLKTCMSLDESVYPTALIKFILAGSQPDVLDTKSEEETLKTTPEKVGSHPNQVVDATSILGQAFSTLPHQWGFPGANLVHSETLAKGSVSPEDLGAITMLNQPETLGMDMDAPPDLPIFLPMPPNTVATYSCLLSGPEPHTSASCGLEVQGATPILTLDSGHTPQPPQNSELSSVPLVIAANSTQAEKQFGANLFPANIQGFTIAPENELQHAPLDLTQGSHAAPSKLEGGISHVSMTSSADDVKATMMSMPVTRASTSSLPCKSMPPTVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSRVQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDYSRRGHGEEEQRLGLFTHPLENVRKNAGGMTGIEVEKWTPNKKSHLAAGQVKGSRGANLAGVENSQPSEDDKQQTNPSPTFAQTVRNGMKNVHCLPTDTDLPLNKLSHEEFSKVLGNNSSKLLTDPSDCKDAMSDTTSREGCDHLKGPRNIVLFQKPGLNCRSGAESTIFNNHPDTHSTGSQHQPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSENSSPSKTEKDEETDQRTASLLNSCKDVLHSVRKDFQDPNVQGKSLHHDTVVFNGQNRAFKSPDSFATNQALIKSHEYSRSPTAGKSIASGKAPLDSSKNSCPLPEESQNLENFSQVLICDQNLNSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINPEFRNAESTPESLVAKNTQQKHSQEKRMVQQKPPSSIQNKPIVPSTKPKKTQKKARPTPYTSKRKKKAPAHSSQESDQKKQERLAIEYSKMHDIWISSQFQRFGQSGPRSLPVLLRNIPIFDQILKPVTQSKTSSHPNKLFPPINQIKFTRNPELAKETVKVEPSDSLPTCQFKMESSGQAFSEPADNSQGQPMVNVDQKAHPLPQSPPSNQCANIMAGAAQTHFHLGARKNLVHQIPPPTLPGISPDPLLPDPASALRKGNVLSSNGITVVTTKHEEAQTSSNGPSDPTTDSAQADFNGNVMDFLSMPAKKLIAELKEQEAATCQCARPDGGPQKDKGPYYTHLGAGPSVAAVRELMETRYGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKVICLVRERVDHYCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGTNPKTCGASFSFGCSWSMYFNGCKFGRSANPRKFRLAPNYPLHDYYKRITGRCSEGSDVKTGWIIPERKTLISREEKQLEENLQDLATVLAPVYKQMAPVAYQNQVEYEDIAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMNNGSTVVCTLIREDGRDRSVPGDEQLHVLPLYRLADTDEFGSVEGMKAKIQSGAIQVNGPTRKRRLCFSEPVPRCGKRAKMKEKDKKSGATGLSKKRNSACTKGAPGSHNTKIYSSASSTPHPVKDESTDFCPLQASSTETSTCMYNKTASGGFPETSSILHCTMPSGAHSGANAAPGECTGIVQTAAEVAALPHQSLPIADSPLHAEPLTSPPDHLTSNQSNQQLPFLSSPQKLASCQVEDERHSETDEPPQPEDGNIPQLDEFWSDSEEIYNDPAFGGVAIAPIHGSVLIECARKELHATTSLSSPKREVPFRVSLVFYQHKNLNKPNHGFDINRIKCKCKKVTKKPPADREDPDLSPETNLSYQIPSRVASTLTSDNVVTVSPYALTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_006223880.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006223880.2

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCAC AAAGCCTTCC AAATCAGTCA AAACAAAGCT CCAGAAGAAG 
AGCAGCCAGA TGAAGAAGAA GATATCAAAG CAGGCTGTCA AACATGGGGC ATCAGGGAAG 
GCGCTGACCC CTGGAAAACT GAAGCAATTA ATCAGAAGAA GAGATGGTAA GAAGGAAACG 
GAAGTCAAAA CCCCCACACT GCCGCAGAGT CTTCTGACGA GAGCTGGGGC TGCCCGGTTG 
AATCAGGATC GAAACCAAGT TCTTTTTCAG AACCCAGAGT CCTTAACTTG CAATGGGTTT 
ACAATGGCTC TTCGGAGAAC CTCTCTTAGC TGGCGACTCT CCCAACGCCC AGTAGTCACA 
CCCAAACCTA AGAAAGTGCC ACCTTCAAAG AAGCAGTGTG TACATAATGT CCAGGATGAT 
CCAGGGGTAA AGCACTCCGG AAATGATTCA GGCCCCAGCC AAGATGCTGT CATGTCTCCA 
GATACAGAAA ACCTAACTGG TGAACAGAAC ATGTGTTTAG TTGAAGGCGA GAGCCAAGAG 
ATAACCCAGT CTTGCTCTCA AACAGTAGAA GATTCCCAAA GCTGTATTTC TGAATATGGG 
AACCTTGAAG CCGGAATTTC TTGGTCACTG GAAGGGACAC ACAGTGAAGG ACTACTCTCT 
CATCAGACAT CTAATAATGT GAGCAATTCC CATCCGGGGT GTGCTCCATC GCCCCAAAGA 
TCAACTTCTG AAGTTACCTC TGAGAAAAAT GCCAGTGATC AGTTTGTAGA CTTGTGCTCA 
CCAGTAGAGT CTCCCAAGCT GTCTGATCCT TCTCAGAATC CTATGGGAAG CGAGCATAAT 
TGCTTTCCTG ACTCTAGTTT TAGGATCGTT CCTGAATTGG ACCTTAAAAC CTGCATGTCT 
CTTGATGAGT CTGTATATCC CACAGCTCTG ATAAAATTCA TCTTGGCAGG CTCACAGCCG 
GATGTCCTTG ATACTAAGTC CGAAGAAGAA ACCCTTAAAA CTACCCCAGA AAAAGTGGGT 
AGCCACCCAA ATCAAGTTGT GGATGCCACC TCTATCCTAG GACAGGCCTT TAGTACTCTC 
CCGCATCAGT GGGGATTTCC TGGTGCTAAC TTAGTTCATA GTGAGACCCT GGCCAAGGGC 
TCAGTCTCAC CAGAGGATCT TGGTGCTATT ACTATGCTAA ACCAACCAGA AACTCTGGGT 
ATGGATATGG ATGCTCCCCC GGACCTGCCT ATCTTCCTTC CTATGCCTCC AAACACAGTT 
GCTACCTACA GTTGTCTTCT TTCAGGGCCT GAGCCCCACA CCTCTGCTTC CTGTGGACTT 
GAAGTCCAAG GTGCTACCCC GATTTTAACT TTGGACTCAG GACACACTCC CCAACCCCCA 
CAAAACTCTG AGTTAAGTTC AGTACCTCTG GTAATAGCTG CAAACAGTAC ACAAGCTGAG 
AAGCAGTTTG GTGCCAATCT TTTCCCAGCA AACATACAAG GGTTTACAAT TGCCCCTGAG 
AATGAACTCC AGCATGCTCC ATTAGATCTT ACTCAGGGCT CTCATGCTGC TCCCAGCAAA 
TTGGAAGGAG GGATTTCTCA CGTTAGCATG ACCAGCTCAG CTGATGATGT CAAAGCCACA 
ATGATGTCTA TGCCTGTTAC ACGAGCTAGT ACCTCTTCTC TCCCATGTAA ATCTATGCCA 
CCAACTGTGG AGAGGAGGAA GCGCAAGGCC TGTGGGGTCT GTGAGCCGTG CCAGCAGAAG 
GCCAACTGTG GGGAATGCAC CTACTGCAAG AACAGGAAGA ATAGCCACCA GATCTGTAAG 
AAGAGGAAAT GCGAGGTGCT CAAAAAGAAG CCAGAGGCCA CCTCACGGGT ACAGGTTACA 
AAAGAAAACA AGAGGCCCCA GAGGGAAAAG AAGCCCAAAG TTTTAAAGAC AGACTTTAAC 
AACAAACCAG TAAATGGCCC CAAATCAGAG TCCATGGACT ACAGTAGACG TGGTCATGGG 
GAAGAAGAGC AAAGGTTGGG TTTGTTCACA CATCCCCTTG AAAATGTAAG GAAGAATGCA 
GGAGGCATGA CGGGAATTGA AGTGGAGAAG TGGACACCGA ACAAGAAATC ACACTTAGCA 
GCTGGTCAAG TCAAAGGAAG CCGAGGTGCA AATTTAGCTG GAGTTGAAAA TTCTCAACCC 
TCTGAAGATG ACAAGCAACA AACCAACCCT TCCCCGACAT TCGCACAGAC TGTAAGAAAC 
GGCATGAAAA ATGTACACTG TTTGCCCACT GACACAGACC TTCCACTCAA CAAATTGAGT 
CATGAGGAAT TTAGCAAGGT ATTGGGAAAT AATTCCTCTA AATTGCTGAC TGATCCCTCA 
GATTGCAAGG ATGCCATGAG TGACACTACC TCTAGGGAGG GGTGTGATCA TCTCAAGGGG 
CCGAGGAACA TCGTGCTCTT CCAGAAGCCT GGCTTAAACT GCAGATCTGG TGCAGAATCC 
ACCATCTTTA ATAATCACCC TGATACACAC AGTACTGGTA GCCAGCATCA ACCCCCTGAG 
AAGGTACCCA ACAAAGAACC CAAAGATGGC TCTCCAGTTC AGCCAAGTCT TTTATCCCTA 
ATGAAAGACA GGAGATTAAC GCTGGAACAA GTAGTAGCCA TAGAAGCACT GACTCAGCTC 
TCAGAAGCTC CCTCAGAGAA CTCCTCCCCA TCCAAGACAG AGAAGGATGA AGAAACTGAT 
CAGAGAACAG CTAGCCTACT TAATAGCTGC AAGGATGTCC TTCATTCAGT GAGAAAAGAC 
TTCCAAGACC CGAATGTTCA AGGAAAGAGT CTTCACCATG ACACTGTTGT CTTTAATGGC 
CAGAACAGAG CGTTCAAGTC ACCAGATAGT TTTGCTACTA ACCAAGCACT TATAAAGTCA 
CATGAATATT CAAGGTCACC TACAGCTGGT AAAAGCATTG CTAGTGGGAA AGCTCCCCTT 
GATAGTTCTA AGAATTCATG TCCATTACCA GAAGAAAGTC AAAATCTTGA AAACTTCAGT 
CAGGTACTAA TCTGTGATCA GAATTTGAAT TCCCATGATC CATCATGCCA GGATGCACCC 
TATAGTCAAA TTGAAGAAGA TGTCGCAGCA CAATTAACCC AACTTGCATC CACCATTAAT 
CACATCAACC CAGAGTTCAG GAATGCTGAA AGTACACCAG AAAGCCTTGT TGCAAAGAAC 
ACACAGCAAA AACATAGTCA GGAGAAACGC ATGGTACAAC AGAAACCACC TTCAAGTATA 
CAAAATAAAC CTATTGTACC ATCAACAAAG CCAAAGAAGA CACAGAAAAA GGCAAGACCA 
ACTCCATACA CAAGCAAGCG GAAAAAGAAA GCCCCAGCCC ACAGCTCTCA AGAAAGTGAT 
CAGAAGAAGC AAGAGCGGCT AGCTATAGAG TATAGCAAGA TGCATGACAT TTGGATATCA 
TCCCAGTTTC AGAGGTTTGG CCAGTCTGGT CCCCGTAGTC TTCCTGTTCT GTTGAGAAAT 
ATTCCCATCT TTGACCAAAT ATTAAAGCCT GTGACTCAGA GTAAAACATC CTCACACCCC 
AATAAGTTAT TTCCTCCTAT CAATCAGATA AAATTCACAA GAAACCCTGA ATTGGCAAAA 
GAGACAGTGA AGGTTGAACC ATCAGATTCT CTGCCAACAT GCCAATTCAA GATGGAATCC 
AGTGGGCAGG CATTTTCAGA GCCGGCAGAT AATTCTCAGG GACAGCCCAT GGTAAATGTC 
GATCAGAAAG CTCACCCTCT GCCCCAGTCC CCTCCATCTA ACCAGTGTGC TAATATAATG 
GCTGGTGCTG CCCAAACACA TTTTCATCTA GGCGCTCGAA AGAACCTGGT GCATCAGATT 
CCACCACCGA CGTTGCCTGG TATCTCTCCT GACCCACTCT TACCAGACCC AGCATCAGCC 
CTCAGGAAAG GGAACGTGTT AAGTTCCAAT GGAATTACAG TTGTCACCAC AAAGCATGAA 
GAAGCTCAAA CATCAAGCAA TGGCCCATCG GATCCCACCA CAGACAGTGC ACAGGCAGAT 
TTTAATGGGA ACGTCATGGA CTTCCTTAGC ATGCCTGCAA AAAAACTGAT AGCTGAATTG 
AAGGAACAGG AAGCTGCAAC CTGTCAATGT GCTCGTCCTG ATGGAGGCCC ACAAAAAGAC 
AAAGGCCCGT ATTATACACA CCTTGGGGCA GGACCAAGTG TGGCTGCTGT CAGGGAGCTC 
ATGGAGACTA GGTATGGCCA GAAGGGAAAA GCAATCCGGA TTGAGAAGAT AGTGTTCACG 
GGGAAGGAAG GGAAGAGCTC TCAGGGCTGC CCGGTCGCCA AGTGGGTGAT TAGAAGAAGT 
GGTCCTGAAG AGAAGGTTAT TTGTTTGGTT CGTGAGCGTG TGGACCATTA CTGTTCAACG 
GCTGTGATAG TTGTTCTTAT CCTCCTGTGG GAAGGTATCC CTCGACTAAT GGCTGACCGC 
CTGTACAAGG AGCTCACTGA GAACTTGAGG TCCTACAGCG GACATCCCAC TGACCGAAGA 
TGTACCCTCA ACAAAAAACG TACCTGCACC TGTCAAGGCA CCAACCCAAA AACATGCGGA 
GCGTCTTTCT CCTTTGGCTG TTCGTGGAGC ATGTATTTCA ACGGCTGTAA GTTTGGGAGA 
AGTGCAAACC CCAGAAAATT CAGACTTGCT CCAAACTATC CCTTACATGA CTACTATAAG 
AGAATTACTG GAAGGTGTTC TGAAGGAAGT GATGTGAAAA CTGGATGGAT CATTCCAGAA 
CGAAAGACCC TCATAAGCAG AGAGGAAAAA CAGCTTGAGG AGAATTTACA AGACTTGGCT 
ACAGTATTAG CTCCAGTTTA TAAGCAGATG GCGCCAGTTG CTTATCAAAA TCAGGTGGAA 
TATGAAGACA TTGCTGGAGA CTGTCGACTT GGTAATGAAG AGGGGCGTCC TTTCTCTGGT 
GTCACCTGTT GCATGGATTT CTGTGCTCAT TCTCACAAGG ACATTCACAA CATGAACAAT 
GGAAGCACTG TGGTTTGTAC ATTGATTCGA GAAGATGGCC GTGACAGAAG TGTTCCTGGG 
GATGAGCAGC TACACGTCCT GCCACTATAC CGGCTTGCAG ACACTGATGA ATTTGGCTCC 
GTGGAAGGGA TGAAAGCCAA GATCCAATCT GGGGCCATCC AAGTCAATGG GCCAACCAGG 
AAGAGGCGAC TGTGTTTCAG TGAGCCTGTT CCTCGATGTG GGAAGAGGGC CAAGATGAAG 
GAGAAGGACA AGAAATCAGG TGCTACTGGC CTCAGCAAGA AGAGGAACTC TGCCTGCACA 
AAGGGAGCCC CAGGTTCGCA CAACACTAAG ATCTATTCAT CAGCCTCATC TACTCCTCAC 
CCAGTGAAAG ATGAATCTAC AGACTTCTGT CCCCTGCAGG CTTCCTCCAC AGAAACATCT 
ACCTGTATGT ACAATAAAAC AGCCTCAGGT GGCTTTCCAG AAACAAGTAG CATTCTCCAC 
TGCACAATGC CTTCTGGAGC ACACAGTGGT GCTAATGCAG CTCCTGGGGA ATGTACTGGA 
ATTGTGCAGA CTGCTGCTGA AGTGGCTGCT CTTCCTCACC AATCTCTTCC CATAGCAGAT 
TCTCCCCTTC ATGCTGAGCC TCTCACAAGT CCACCTGATC ATCTAACTTC TAACCAGTCA 
AACCAGCAGC TCCCTTTCCT CAGCTCTCCT CAGAAACTGG CTTCCTGTCA GGTGGAAGAT 
GAACGGCATT CGGAAACAGA TGAGCCTCCT CAACCCGAGG ATGGTAACAT CCCTCAACTT 
GATGAATTTT GGTCAGACAG TGAGGAGATC TATAATGATC CTGCCTTTGG TGGCGTGGCG 
ATAGCACCCA TTCATGGCTC AGTGCTGATT GAGTGTGCCC GGAAAGAGCT TCATGCTACC 
ACCTCTTTGA GTTCTCCCAA ACGAGAGGTC CCTTTTCGTG TGTCCCTTGT ATTCTACCAG 
CACAAAAATC TAAATAAGCC TAATCACGGT TTTGATATCA ACAGAATTAA GTGTAAATGT 
AAAAAAGTAA CAAAAAAACC CCCCGCAGAC AGGGAGGATC CTGATTTATC TCCTGAAACC 
AATTTATCAT ACCAAATTCC TTCTCGAGTT GCATCAACCT TAACCAGCGA CAACGTTGTT 
ACCGTGTCCC CTTATGCTCT CACTCACGTC GCGGGACCCT ACAACCGTTG GGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tet1 ( XM_017601794.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457283.1 Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.

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CloneID	ORa39430
Clone ID Related Accession (Same CDS sequence)	XM_017601794.1
Accession Version	XM_017601794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: methylcytosine dioxygenase TET1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGTCTCGGT CCCGCCCCAC AAAGCCTTCC AAATCAGTCA AAACAAAGCT CCAGAAGAAG 
AGCAGCCAGA TGAAGAAGAA GATATCAAAG CAGGCTGTCA AACATGGGGC ATCAGGGAAG 
GCGCTGACCC CTGGAAAACT GAAGCAATTA ATCAGAAGAA GAGATGGTAA GAAGGAAACG 
GAAGTCAAAA CCCCCACACT GCCGCAGAGT CTTCTGACGA GAGCTGGGGC TGCCCGGTTG 
AATCAGGATC GAAACCAAGT TCTTTTTCAG AACCCAGAGT CCTTAACTTG CAATGGGTTT 
ACAATGGCTC TTCGGAGAAC CTCTCTTAGC TGGCGACTCT CCCAACGCCC AGTAGTCACA 
CCCAAACCTA AGAAAGTGCC ACCTTCAAAG AAGCAGTGTG TACATAATGT CCAGGATGAT 
CCAGGGGTAA AGCACTCCGG AAATGATTCA GGCCCCAGCC AAGATGCTGT CATGTCTCCA 
GATACAGAAA ACCTAACTGG TGAACAGAAC ATGTGTTTAG TTGAAGGCGA GAGCCAAGAG 
ATAACCCAGT CTTGCTCTCA AACAGTAGAA GATTCCCAAA GCTGTATTTC TGAATATGGG 
AACCTTGAAG CCGGAATTTC TTGGTCACTG GAAGGGACAC ACAGTGAAGG ACTACTCTCT 
CATCAGACAT CTAATAATGT GAGCAATTCC CATCCGGGGT GTGCTCCATC GCCCCAAAGA 
TCAACTTCTG AAGTTACCTC TGAGAAAAAT GCCAGTGATC AGTTTGTAGA CTTGTGCTCA 
CCAGTAGAGT CTCCCAAGCT GTCTGATCCT TCTCAGAATC CTATGGGAAG CGAGCATAAT 
TGCTTTCCTG ACTCTAGTTT TAGGATCGTT CCTGAATTGG ACCTTAAAAC CTGCATGTCT 
CTTGATGAGT CTGTATATCC CACAGCTCTG ATAAAATTCA TCTTGGCAGG CTCACAGCCG 
GATGTCCTTG ATACTAAGTC CGAAGAAGAA ACCCTTAAAA CTACCCCAGA AAAAGTGGGT 
AGCCACCCAA ATCAAGTTGT GGATGCCACC TCTATCCTAG GACAGGCCTT TAGTACTCTC 
CCGCATCAGT GGGGATTTCC TGGTGCTAAC TTAGTTCATA GTGAGACCCT GGCCAAGGGC 
TCAGTCTCAC CAGAGGATCT TGGTGCTATT ACTATGCTAA ACCAACCAGA AACTCTGGGT 
ATGGATATGG ATGCTCCCCC GGACCTGCCT ATCTTCCTTC CTATGCCTCC AAACACAGTT 
GCTACCTACA GTTGTCTTCT TTCAGGGCCT GAGCCCCACA CCTCTGCTTC CTGTGGACTT 
GAAGTCCAAG GTGCTACCCC GATTTTAACT TTGGACTCAG GACACACTCC CCAACCCCCA 
CAAAACTCTG AGTTAAGTTC AGTACCTCTG GTAATAGCTG CAAACAGTAC ACAAGCTGAG 
AAGCAGTTTG GTGCCAATCT TTTCCCAGCA AACATACAAG GGTTTACAAT TGCCCCTGAG 
AATGAACTCC AGCATGCTCC ATTAGATCTT ACTCAGGGCT CTCATGCTGC TCCCAGCAAA 
TTGGAAGGAG GGATTTCTCA CGTTAGCATG ACCAGCTCAG CTGATGATGT CAAAGCCACA 
ATGATGTCTA TGCCTGTTAC ACGAGCTAGT ACCTCTTCTC TCCCATGTAA ATCTATGCCA 
CCAACTGTGG AGAGGAGGAA GCGCAAGGCC TGTGGGGTCT GTGAGCCGTG CCAGCAGAAG 
GCCAACTGTG GGGAATGCAC CTACTGCAAG AACAGGAAGA ATAGCCACCA GATCTGTAAG 
AAGAGGAAAT GCGAGGTGCT CAAAAAGAAG CCAGAGGCCA CCTCACGGGT ACAGGTTACA 
AAAGAAAACA AGAGGCCCCA GAGGGAAAAG AAGCCCAAAG TTTTAAAGAC AGACTTTAAC 
AACAAACCAG TAAATGGCCC CAAATCAGAG TCCATGGACT ACAGTAGACG TGGTCATGGG 
GAAGAAGAGC AAAGGTTGGG TTTGTTCACA CATCCCCTTG AAAATGTAAG GAAGAATGCA 
GGAGGCATGA CGGGAATTGA AGTGGAGAAG TGGACACCGA ACAAGAAATC ACACTTAGCA 
GCTGGTCAAG TCAAAGGAAG CCGAGGTGCA AATTTAGCTG GAGTTGAAAA TTCTCAACCC 
TCTGAAGATG ACAAGCAACA AACCAACCCT TCCCCGACAT TCGCACAGAC TGTAAGAAAC 
GGCATGAAAA ATGTACACTG TTTGCCCACT GACACAGACC TTCCACTCAA CAAATTGAGT 
CATGAGGAAT TTAGCAAGGT ATTGGGAAAT AATTCCTCTA AATTGCTGAC TGATCCCTCA 
GATTGCAAGG ATGCCATGAG TGACACTACC TCTAGGGAGG GGTGTGATCA TCTCAAGGGG 
CCGAGGAACA TCGTGCTCTT CCAGAAGCCT GGCTTAAACT GCAGATCTGG TGCAGAATCC 
ACCATCTTTA ATAATCACCC TGATACACAC AGTACTGGTA GCCAGCATCA ACCCCCTGAG 
AAGGTACCCA ACAAAGAACC CAAAGATGGC TCTCCAGTTC AGCCAAGTCT TTTATCCCTA 
ATGAAAGACA GGAGATTAAC GCTGGAACAA GTAGTAGCCA TAGAAGCACT GACTCAGCTC 
TCAGAAGCTC CCTCAGAGAA CTCCTCCCCA TCCAAGACAG AGAAGGATGA AGAAACTGAT 
CAGAGAACAG CTAGCCTACT TAATAGCTGC AAGGATGTCC TTCATTCAGT GAGAAAAGAC 
TTCCAAGACC CGAATGTTCA AGGAAAGAGT CTTCACCATG ACACTGTTGT CTTTAATGGC 
CAGAACAGAG CGTTCAAGTC ACCAGATAGT TTTGCTACTA ACCAAGCACT TATAAAGTCA 
CATGAATATT CAAGGTCACC TACAGCTGGT AAAAGCATTG CTAGTGGGAA AGCTCCCCTT 
GATAGTTCTA AGAATTCATG TCCATTACCA GAAGAAAGTC AAAATCTTGA AAACTTCAGT 
CAGGTACTAA TCTGTGATCA GAATTTGAAT TCCCATGATC CATCATGCCA GGATGCACCC 
TATAGTCAAA TTGAAGAAGA TGTCGCAGCA CAATTAACCC AACTTGCATC CACCATTAAT 
CACATCAACC CAGAGTTCAG GAATGCTGAA AGTACACCAG AAAGCCTTGT TGCAAAGAAC 
ACACAGCAAA AACATAGTCA GGAGAAACGC ATGGTACAAC AGAAACCACC TTCAAGTATA 
CAAAATAAAC CTATTGTACC ATCAACAAAG CCAAAGAAGA CACAGAAAAA GGCAAGACCA 
ACTCCATACA CAAGCAAGCG GAAAAAGAAA GCCCCAGCCC ACAGCTCTCA AGAAAGTGAT 
CAGAAGAAGC AAGAGCGGCT AGCTATAGAG TATAGCAAGA TGCATGACAT TTGGATATCA 
TCCCAGTTTC AGAGGTTTGG CCAGTCTGGT CCCCGTAGTC TTCCTGTTCT GTTGAGAAAT 
ATTCCCATCT TTGACCAAAT ATTAAAGCCT GTGACTCAGA GTAAAACATC CTCACACCCC 
AATAAGTTAT TTCCTCCTAT CAATCAGATA AAATTCACAA GAAACCCTGA ATTGGCAAAA 
GAGACAGTGA AGGTTGAACC ATCAGATTCT CTGCCAACAT GCCAATTCAA GATGGAATCC 
AGTGGGCAGG CATTTTCAGA GCCGGCAGAT AATTCTCAGG GACAGCCCAT GGTAAATGTC 
GATCAGAAAG CTCACCCTCT GCCCCAGTCC CCTCCATCTA ACCAGTGTGC TATATATGGC 
TGTGCTGCCC AACCATNTCA TCTAGCGCTC GAAAGAACCN NTGTGCATCA GATCCACCCA 
CCGACGNNTG GCTGTATCTC TCCTGACCCA CTCTTACCAG ACCCAGCATC AGCCCTCAGG 
AAAGGGAACG TGTTAAGTTC CAATGGAATT ACAGTTGTCA CCACAAAGCA TGAAGAAGCT 
CAAACATCAA GCAATGGCCC ATCGGATCCC ACCACAGACA GTGCACAGGC AGATTTTAAT 
GGGAACGTCA TGGACTTCCT TAGCATGCCT GCAAAAAAAC TGATAGCTGA ATTGAAGGAA 
CAGGAAGCTG CAACCTGTCA ATGTGCTCGT CCTGATGGAG GCCCACAAAA AGACAAAGGC 
CCGTATTATA CACACCTTGG GGCAGGACCA AGTGTGGCTG CTGTCAGGGA GCTCATGGAG 
ACTAGGTATG GCCAGAAGGG AAAAGCAATC CGGATTGAGA AGATAGTGTT CACGGGGAAG 
GAAGGGAAGA GCTCTCAGGG CTGCCCGGTC GCCAAGTGGG TGATTAGAAG AAGTGGTCCT 
GAAGAGAAGG TTATTTGTTT GGTTCGTGAG CGTGTGGACC ATTACTGTTC AACGGCTGTG 
ATAGTTGTTC TTATCCTCCT GTGGGAAGGT ATCCCTCGAC TAATGGCTGA CCGCCTGTAC 
AAGGAGCTCA CTGAGAACTT GAGGTCCTAC AGCGGACATC CCACTGACCG AAGATGTACC 
CTCAACAAAA AACGTACCTG CACCTGTCAA GGCACCAACC CAAAAACATG CGGAGCGTCT 
TTCTCCTTTG GCTGTTCGTG GAGCATGTAT TTCAACGGCT GTAAGTTTGG GAGAAGTGCA 
AACCCCAGAA AATTCAGACT TGCTCCAAAC TATCCCTTAC ATGACTACTA TAAGAGAATT 
ACTGGAAGGT GTTCTGAAGG AAGTGATGTG AAAACTGGAT GGATCATTCC AGAACGAAAG 
ACCCTCATAA GCAGAGAGGA AAAACAGCTT GAGGAGAATT TACAAGACTT GGCTACAGTA 
TTAGCTCCAG TTTATAAGCA GATGGCGCCA GTTGCTTATC AAAATCAGGT GGAATATGAA 
GACATTGCTG GAGACTGTCG ACTTGGTAAT GAAGAGGGGC GTCCTTTCTC TGGTGTCACC 
TGTTGCATGG ATTTCTGTGC TCATTCTCAC AAGGACATTC ACAACATGAA CAATGGAAGC 
ACTGTGGTTT GTACATTGAT TCGAGAAGAT GGCCGTGACA GAAGTGTTCC TGGGGATGAG 
CAGCTACACG TCCTGCCACT ATACCGGCTT GCAGACACTG ATGAATTTGG CTCCGTGGAA 
GGGATGAAAG CCAAGATCCA ATCTGGGGCC ATCCAAGTCA ATGGGCCAAC CAGGAAGAGG 
CGACTGTGTT TCAGTGAGCC TGTTCCTCGA TGTGGGAAGA GGGCCAAGAT GAAGGAGAAG 
GACAAGAAAT CAGGTGCTAC TGGCCTCAGC AAGAAGAGGA ACTCTGCCTG CACAAAGGGA 
GCCCCAGGTT CGCACAACAC TAAGATCTAT TCATCAGCCT CATCTACTCC TCACCCAGTG 
AAAGATGAAT CTACAGACTT CTGTCCCCTG CAGGCTTCCT CCACAGAAAC ATCTACCTGT 
ATGTACAATA AAACAGCCTC AGGTGGCTTT CCAGAAACAA GTAGCATTCT CCACTGCACA 
ATGCCTTCTG GAGCACACAG TGGTGCTAAT GCAGCTCCTG GGGAATGTAC TGGAATTGTG 
CAGACTGCTG CTGAAGTGGC TGCTCTTCCT CACCAATCTC TTCCCATAGC AGATTCTCCC 
CTTCATGCTG AGCCTCTCAC AAGTCCACCT GATCATCTAA CTTCTAACCA GTCAAACCAG 
CAGCTCCCTT TCCTCAGCTC TCCTCAGAAA CTGGCTTCCT GTCAGGTGGA AGATGAACGG 
CATTCGGAAA CAGATGAGCC TCCTCAACCC GAGGATGGTA ACATCCCTCA ACTTGATGAA 
TTTTGGTCAG ACAGTGAGGA GATCTATAAT GATCCTGCCT TTGGTGGCGT GGCGATAGCA 
CCCATTCATG GCTCAGTGCT GATTGAGTGT GCCCGGAAAG AGCTTCATGC TACCACCTCT 
TTGAGTTCTC CCAAACGAGA GGTCCCTTTT CGTGTGTCCC TTGTATTCTA CCAGCACAAA 
AATCTAAATA AGCCTAATCA CGGTTTTGAT ATCAACAGAA TTAAGTGTAA ATGTAAAAAA 
GTAACAAAAA AACCCCCCGC AGACAGGGAG GATCCTGATT TATCTCCTGA AACCAATTTA 
TCATACCAAA TTCCTTCTCG AGTTGCATCA ACCTTAACCA GCGACAACGT TGTTACCGTG 
TCCCCTTATG CTCTCACTCA CGTCGCGGGA CCCTACAACC GTTGGGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457283.1
CDS	137..6307
Translation	MSRSRPTKPSKSVKTKLQKKSSQMKKKISKQAVKHGASGKALTPGKLKQLIRRRDGKKETEVKTPTLPQSLLTRAGAARLNQDRNQVLFQNPESLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCVHNVQDDPGVKHSGNDSGPSQDAVMSPDTENLTGEQNMCLVEGESQEITQSCSQTVEDSQSCISEYGNLEAGISWSLEGTHSEGLLSHQTSNNVSNSHPGCAPSPQRSTSEVTSEKNASDQFVDLCSPVESPKLSDPSQNPMGSEHNCFPDSSFRIVPELDLKTCMSLDESVYPTALIKFILAGSQPDVLDTKSEEETLKTTPEKVGSHPNQVVDATSILGQAFSTLPHQWGFPGANLVHSETLAKGSVSPEDLGAITMLNQPETLGMDMDAPPDLPIFLPMPPNTVATYSCLLSGPEPHTSASCGLEVQGATPILTLDSGHTPQPPQNSELSSVPLVIAANSTQAEKQFGANLFPANIQGFTIAPENELQHAPLDLTQGSHAAPSKLEGGISHVSMTSSADDVKATMMSMPVTRASTSSLPCKSMPPTVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSRVQVTKENKRPQREKKPKVLKTDFNNKPVNGPKSESMDYSRRGHGEEEQRLGLFTHPLENVRKNAGGMTGIEVEKWTPNKKSHLAAGQVKGSRGANLAGVENSQPSEDDKQQTNPSPTFAQTVRNGMKNVHCLPTDTDLPLNKLSHEEFSKVLGNNSSKLLTDPSDCKDAMSDTTSREGCDHLKGPRNIVLFQKPGLNCRSGAESTIFNNHPDTHSTGSQHQPPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSENSSPSKTEKDEETDQRTASLLNSCKDVLHSVRKDFQDPNVQGKSLHHDTVVFNGQNRAFKSPDSFATNQALIKSHEYSRSPTAGKSIASGKAPLDSSKNSCPLPEESQNLENFSQVLICDQNLNSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINPEFRNAESTPESLVAKNTQQKHSQEKRMVQQKPPSSIQNKPIVPSTKPKKTQKKARPTPYTSKRKKKAPAHSSQESDQKKQERLAIEYSKMHDIWISSQFQRFGQSGPRSLPVLLRNIPIFDQILKPVTQSKTSSHPNKLFPPINQIKFTRNPELAKETVKVEPSDSLPTCQFKMESSGQAFSEPADNSQGQPMVNVDQKAHPLPQSPPSNQCAIYGCAAQPnullHLALERTnullVHQIHPPTnullGCISPDPLLPDPASALRKGNVLSSNGITVVTTKHEEAQTSSNGPSDPTTDSAQADFNGNVMDFLSMPAKKLIAELKEQEAATCQCARPDGGPQKDKGPYYTHLGAGPSVAAVRELMETRYGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKVICLVRERVDHYCSTAVIVVLILLWEGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGTNPKTCGASFSFGCSWSMYFNGCKFGRSANPRKFRLAPNYPLHDYYKRITGRCSEGSDVKTGWIIPERKTLISREEKQLEENLQDLATVLAPVYKQMAPVAYQNQVEYEDIAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMNNGSTVVCTLIREDGRDRSVPGDEQLHVLPLYRLADTDEFGSVEGMKAKIQSGAIQVNGPTRKRRLCFSEPVPRCGKRAKMKEKDKKSGATGLSKKRNSACTKGAPGSHNTKIYSSASSTPHPVKDESTDFCPLQASSTETSTCMYNKTASGGFPETSSILHCTMPSGAHSGANAAPGECTGIVQTAAEVAALPHQSLPIADSPLHAEPLTSPPDHLTSNQSNQQLPFLSSPQKLASCQVEDERHSETDEPPQPEDGNIPQLDEFWSDSEEIYNDPAFGGVAIAPIHGSVLIECARKELHATTSLSSPKREVPFRVSLVFYQHKNLNKPNHGFDINRIKCKCKKVTKKPPADREDPDLSPETNLSYQIPSRVASTLTSDNVVTVSPYALTHVAGPYNRWV

Target ORF information:

RefSeq Version	XM_017601794.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tet methylcytosine dioxygenase 1 (Tet1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601794.1

ORF Insert Sequence:

ATGTCTCGGT CCCGCCCCAC AAAGCCTTCC AAATCAGTCA AAACAAAGCT CCAGAAGAAG 
AGCAGCCAGA TGAAGAAGAA GATATCAAAG CAGGCTGTCA AACATGGGGC ATCAGGGAAG 
GCGCTGACCC CTGGAAAACT GAAGCAATTA ATCAGAAGAA GAGATGGTAA GAAGGAAACG 
GAAGTCAAAA CCCCCACACT GCCGCAGAGT CTTCTGACGA GAGCTGGGGC TGCCCGGTTG 
AATCAGGATC GAAACCAAGT TCTTTTTCAG AACCCAGAGT CCTTAACTTG CAATGGGTTT 
ACAATGGCTC TTCGGAGAAC CTCTCTTAGC TGGCGACTCT CCCAACGCCC AGTAGTCACA 
CCCAAACCTA AGAAAGTGCC ACCTTCAAAG AAGCAGTGTG TACATAATGT CCAGGATGAT 
CCAGGGGTAA AGCACTCCGG AAATGATTCA GGCCCCAGCC AAGATGCTGT CATGTCTCCA 
GATACAGAAA ACCTAACTGG TGAACAGAAC ATGTGTTTAG TTGAAGGCGA GAGCCAAGAG 
ATAACCCAGT CTTGCTCTCA AACAGTAGAA GATTCCCAAA GCTGTATTTC TGAATATGGG 
AACCTTGAAG CCGGAATTTC TTGGTCACTG GAAGGGACAC ACAGTGAAGG ACTACTCTCT 
CATCAGACAT CTAATAATGT GAGCAATTCC CATCCGGGGT GTGCTCCATC GCCCCAAAGA 
TCAACTTCTG AAGTTACCTC TGAGAAAAAT GCCAGTGATC AGTTTGTAGA CTTGTGCTCA 
CCAGTAGAGT CTCCCAAGCT GTCTGATCCT TCTCAGAATC CTATGGGAAG CGAGCATAAT 
TGCTTTCCTG ACTCTAGTTT TAGGATCGTT CCTGAATTGG ACCTTAAAAC CTGCATGTCT 
CTTGATGAGT CTGTATATCC CACAGCTCTG ATAAAATTCA TCTTGGCAGG CTCACAGCCG 
GATGTCCTTG ATACTAAGTC CGAAGAAGAA ACCCTTAAAA CTACCCCAGA AAAAGTGGGT 
AGCCACCCAA ATCAAGTTGT GGATGCCACC TCTATCCTAG GACAGGCCTT TAGTACTCTC 
CCGCATCAGT GGGGATTTCC TGGTGCTAAC TTAGTTCATA GTGAGACCCT GGCCAAGGGC 
TCAGTCTCAC CAGAGGATCT TGGTGCTATT ACTATGCTAA ACCAACCAGA AACTCTGGGT 
ATGGATATGG ATGCTCCCCC GGACCTGCCT ATCTTCCTTC CTATGCCTCC AAACACAGTT 
GCTACCTACA GTTGTCTTCT TTCAGGGCCT GAGCCCCACA CCTCTGCTTC CTGTGGACTT 
GAAGTCCAAG GTGCTACCCC GATTTTAACT TTGGACTCAG GACACACTCC CCAACCCCCA 
CAAAACTCTG AGTTAAGTTC AGTACCTCTG GTAATAGCTG CAAACAGTAC ACAAGCTGAG 
AAGCAGTTTG GTGCCAATCT TTTCCCAGCA AACATACAAG GGTTTACAAT TGCCCCTGAG 
AATGAACTCC AGCATGCTCC ATTAGATCTT ACTCAGGGCT CTCATGCTGC TCCCAGCAAA 
TTGGAAGGAG GGATTTCTCA CGTTAGCATG ACCAGCTCAG CTGATGATGT CAAAGCCACA 
ATGATGTCTA TGCCTGTTAC ACGAGCTAGT ACCTCTTCTC TCCCATGTAA ATCTATGCCA 
CCAACTGTGG AGAGGAGGAA GCGCAAGGCC TGTGGGGTCT GTGAGCCGTG CCAGCAGAAG 
GCCAACTGTG GGGAATGCAC CTACTGCAAG AACAGGAAGA ATAGCCACCA GATCTGTAAG 
AAGAGGAAAT GCGAGGTGCT CAAAAAGAAG CCAGAGGCCA CCTCACGGGT ACAGGTTACA 
AAAGAAAACA AGAGGCCCCA GAGGGAAAAG AAGCCCAAAG TTTTAAAGAC AGACTTTAAC 
AACAAACCAG TAAATGGCCC CAAATCAGAG TCCATGGACT ACAGTAGACG TGGTCATGGG 
GAAGAAGAGC AAAGGTTGGG TTTGTTCACA CATCCCCTTG AAAATGTAAG GAAGAATGCA 
GGAGGCATGA CGGGAATTGA AGTGGAGAAG TGGACACCGA ACAAGAAATC ACACTTAGCA 
GCTGGTCAAG TCAAAGGAAG CCGAGGTGCA AATTTAGCTG GAGTTGAAAA TTCTCAACCC 
TCTGAAGATG ACAAGCAACA AACCAACCCT TCCCCGACAT TCGCACAGAC TGTAAGAAAC 
GGCATGAAAA ATGTACACTG TTTGCCCACT GACACAGACC TTCCACTCAA CAAATTGAGT 
CATGAGGAAT TTAGCAAGGT ATTGGGAAAT AATTCCTCTA AATTGCTGAC TGATCCCTCA 
GATTGCAAGG ATGCCATGAG TGACACTACC TCTAGGGAGG GGTGTGATCA TCTCAAGGGG 
CCGAGGAACA TCGTGCTCTT CCAGAAGCCT GGCTTAAACT GCAGATCTGG TGCAGAATCC 
ACCATCTTTA ATAATCACCC TGATACACAC AGTACTGGTA GCCAGCATCA ACCCCCTGAG 
AAGGTACCCA ACAAAGAACC CAAAGATGGC TCTCCAGTTC AGCCAAGTCT TTTATCCCTA 
ATGAAAGACA GGAGATTAAC GCTGGAACAA GTAGTAGCCA TAGAAGCACT GACTCAGCTC 
TCAGAAGCTC CCTCAGAGAA CTCCTCCCCA TCCAAGACAG AGAAGGATGA AGAAACTGAT 
CAGAGAACAG CTAGCCTACT TAATAGCTGC AAGGATGTCC TTCATTCAGT GAGAAAAGAC 
TTCCAAGACC CGAATGTTCA AGGAAAGAGT CTTCACCATG ACACTGTTGT CTTTAATGGC 
CAGAACAGAG CGTTCAAGTC ACCAGATAGT TTTGCTACTA ACCAAGCACT TATAAAGTCA 
CATGAATATT CAAGGTCACC TACAGCTGGT AAAAGCATTG CTAGTGGGAA AGCTCCCCTT 
GATAGTTCTA AGAATTCATG TCCATTACCA GAAGAAAGTC AAAATCTTGA AAACTTCAGT 
CAGGTACTAA TCTGTGATCA GAATTTGAAT TCCCATGATC CATCATGCCA GGATGCACCC 
TATAGTCAAA TTGAAGAAGA TGTCGCAGCA CAATTAACCC AACTTGCATC CACCATTAAT 
CACATCAACC CAGAGTTCAG GAATGCTGAA AGTACACCAG AAAGCCTTGT TGCAAAGAAC 
ACACAGCAAA AACATAGTCA GGAGAAACGC ATGGTACAAC AGAAACCACC TTCAAGTATA 
CAAAATAAAC CTATTGTACC ATCAACAAAG CCAAAGAAGA CACAGAAAAA GGCAAGACCA 
ACTCCATACA CAAGCAAGCG GAAAAAGAAA GCCCCAGCCC ACAGCTCTCA AGAAAGTGAT 
CAGAAGAAGC AAGAGCGGCT AGCTATAGAG TATAGCAAGA TGCATGACAT TTGGATATCA 
TCCCAGTTTC AGAGGTTTGG CCAGTCTGGT CCCCGTAGTC TTCCTGTTCT GTTGAGAAAT 
ATTCCCATCT TTGACCAAAT ATTAAAGCCT GTGACTCAGA GTAAAACATC CTCACACCCC 
AATAAGTTAT TTCCTCCTAT CAATCAGATA AAATTCACAA GAAACCCTGA ATTGGCAAAA 
GAGACAGTGA AGGTTGAACC ATCAGATTCT CTGCCAACAT GCCAATTCAA GATGGAATCC 
AGTGGGCAGG CATTTTCAGA GCCGGCAGAT AATTCTCAGG GACAGCCCAT GGTAAATGTC 
GATCAGAAAG CTCACCCTCT GCCCCAGTCC CCTCCATCTA ACCAGTGTGC TATATATGGC 
TGTGCTGCCC AACCATNTCA TCTAGCGCTC GAAAGAACCN NTGTGCATCA GATCCACCCA 
CCGACGNNTG GCTGTATCTC TCCTGACCCA CTCTTACCAG ACCCAGCATC AGCCCTCAGG 
AAAGGGAACG TGTTAAGTTC CAATGGAATT ACAGTTGTCA CCACAAAGCA TGAAGAAGCT 
CAAACATCAA GCAATGGCCC ATCGGATCCC ACCACAGACA GTGCACAGGC AGATTTTAAT 
GGGAACGTCA TGGACTTCCT TAGCATGCCT GCAAAAAAAC TGATAGCTGA ATTGAAGGAA 
CAGGAAGCTG CAACCTGTCA ATGTGCTCGT CCTGATGGAG GCCCACAAAA AGACAAAGGC 
CCGTATTATA CACACCTTGG GGCAGGACCA AGTGTGGCTG CTGTCAGGGA GCTCATGGAG 
ACTAGGTATG GCCAGAAGGG AAAAGCAATC CGGATTGAGA AGATAGTGTT CACGGGGAAG 
GAAGGGAAGA GCTCTCAGGG CTGCCCGGTC GCCAAGTGGG TGATTAGAAG AAGTGGTCCT 
GAAGAGAAGG TTATTTGTTT GGTTCGTGAG CGTGTGGACC ATTACTGTTC AACGGCTGTG 
ATAGTTGTTC TTATCCTCCT GTGGGAAGGT ATCCCTCGAC TAATGGCTGA CCGCCTGTAC 
AAGGAGCTCA CTGAGAACTT GAGGTCCTAC AGCGGACATC CCACTGACCG AAGATGTACC 
CTCAACAAAA AACGTACCTG CACCTGTCAA GGCACCAACC CAAAAACATG CGGAGCGTCT 
TTCTCCTTTG GCTGTTCGTG GAGCATGTAT TTCAACGGCT GTAAGTTTGG GAGAAGTGCA 
AACCCCAGAA AATTCAGACT TGCTCCAAAC TATCCCTTAC ATGACTACTA TAAGAGAATT 
ACTGGAAGGT GTTCTGAAGG AAGTGATGTG AAAACTGGAT GGATCATTCC AGAACGAAAG 
ACCCTCATAA GCAGAGAGGA AAAACAGCTT GAGGAGAATT TACAAGACTT GGCTACAGTA 
TTAGCTCCAG TTTATAAGCA GATGGCGCCA GTTGCTTATC AAAATCAGGT GGAATATGAA 
GACATTGCTG GAGACTGTCG ACTTGGTAAT GAAGAGGGGC GTCCTTTCTC TGGTGTCACC 
TGTTGCATGG ATTTCTGTGC TCATTCTCAC AAGGACATTC ACAACATGAA CAATGGAAGC 
ACTGTGGTTT GTACATTGAT TCGAGAAGAT GGCCGTGACA GAAGTGTTCC TGGGGATGAG 
CAGCTACACG TCCTGCCACT ATACCGGCTT GCAGACACTG ATGAATTTGG CTCCGTGGAA 
GGGATGAAAG CCAAGATCCA ATCTGGGGCC ATCCAAGTCA ATGGGCCAAC CAGGAAGAGG 
CGACTGTGTT TCAGTGAGCC TGTTCCTCGA TGTGGGAAGA GGGCCAAGAT GAAGGAGAAG 
GACAAGAAAT CAGGTGCTAC TGGCCTCAGC AAGAAGAGGA ACTCTGCCTG CACAAAGGGA 
GCCCCAGGTT CGCACAACAC TAAGATCTAT TCATCAGCCT CATCTACTCC TCACCCAGTG 
AAAGATGAAT CTACAGACTT CTGTCCCCTG CAGGCTTCCT CCACAGAAAC ATCTACCTGT 
ATGTACAATA AAACAGCCTC AGGTGGCTTT CCAGAAACAA GTAGCATTCT CCACTGCACA 
ATGCCTTCTG GAGCACACAG TGGTGCTAAT GCAGCTCCTG GGGAATGTAC TGGAATTGTG 
CAGACTGCTG CTGAAGTGGC TGCTCTTCCT CACCAATCTC TTCCCATAGC AGATTCTCCC 
CTTCATGCTG AGCCTCTCAC AAGTCCACCT GATCATCTAA CTTCTAACCA GTCAAACCAG 
CAGCTCCCTT TCCTCAGCTC TCCTCAGAAA CTGGCTTCCT GTCAGGTGGA AGATGAACGG 
CATTCGGAAA CAGATGAGCC TCCTCAACCC GAGGATGGTA ACATCCCTCA ACTTGATGAA 
TTTTGGTCAG ACAGTGAGGA GATCTATAAT GATCCTGCCT TTGGTGGCGT GGCGATAGCA 
CCCATTCATG GCTCAGTGCT GATTGAGTGT GCCCGGAAAG AGCTTCATGC TACCACCTCT 
TTGAGTTCTC CCAAACGAGA GGTCCCTTTT CGTGTGTCCC TTGTATTCTA CCAGCACAAA 
AATCTAAATA AGCCTAATCA CGGTTTTGAT ATCAACAGAA TTAAGTGTAA ATGTAAAAAA 
GTAACAAAAA AACCCCCCGC AGACAGGGAG GATCCTGATT TATCTCCTGA AACCAATTTA 
TCATACCAAA TTCCTTCTCG AGTTGCATCA ACCTTAACCA GCGACAACGT TGTTACCGTG 
TCCCCTTATG CTCTCACTCA CGTCGCGGGA CCCTACAACC GTTGGGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Tet1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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