F5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	F5
Entrez Gene ID	304929
Full Name	coagulation factor V
Synonyms	Ac2-120
General protein information	Preferred Names coagulation factor V Names coagulation factor V coagulation factor V (proaccelerin, labile factor)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q23
Summary	may play a role in blood coagulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001047878.1	NP_001041343.1	coagulation factor V

Pathways from BioSystems

Homologies

Bos taurus (cattle)	F5	NP_776304.1
Rattus norvegicus (Norway rat)	F5	NP_001041343.1
Gallus gallus (chicken)	F5	XP_001231901.3
Danio rerio (zebrafish)	f5	NP_001007209.2
Mus musculus (house mouse)	F5	NP_032002.1
Pan troglodytes (chimpanzee)	F5	XP_513984.4
Homo sapiens (human)	F5	NP_000121.2
Macaca mulatta (Rhesus monkey)	F5	XP_001093072.2
Canis lupus familiaris (dog)	F5	XP_005622605.1
Xenopus tropicalis (tropical clawed frog)	f5	XP_002935820.2

Gene Ontology
Bibliography

Related articles in PubMed

The relationship between the levels and function of endothelial progenitor cells and factor V Leiden and protein C deficiency in patients with primary Budd-Chiari syndrome.
Guan ZY, Yu CW, Song T, Gao Y
European review for medical and pharmacological sciences22(9)2742-2750(2018 05)

Association of factor V Leiden, Janus kinase 2, prothrombin, and MTHFR mutations with primary Budd-Chiari syndrome in Egyptian patients.
El Sebay HM, Safan MA, Daoud AA, Tayel SI, Nouh MA, El Shafie S
Journal of gastroenterology and hepatology31(1)235-40(2016 Jan)

Prothrombotic genetic risk factors are associated with an increased risk of liver fibrosis in the general population: The Rotterdam Study.
Plompen EP, Darwish Murad S, Hansen BE, Loth DW, Schouten JN, Taimr P, Hofman A, Uitterlinden AG, Stricker BH, Janssen HL, Leebeek FW
Journal of hepatology63(6)1459-65(2015 Dec)

Plasminogen activator inhibitor-1 4G/5G polymorphism, factor V Leiden, prothrombin mutations and the risk of VTE recurrence.
Sundquist K, Wang X, Svensson PJ, Sundquist J, Hedelius A, Larsson Lönn S, Zöller B, Memon AA
Thrombosis and haemostasis114(6)1156-64(2015 Nov)

How it all starts: Initiation of the clotting cascade.
Smith SA, Travers RJ, Morrissey JH
Critical reviews in biochemistry and molecular biology50(4)326-36(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13664	NM_001047878.1 Latest version!	Rattus norvegicus coagulation factor V (F5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F5 ( NM_001047878.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001041343.1 Rattus norvegicus coagulation factor V (F5), mRNA.

Price & Availability↑

CloneID	ORa13664
Clone ID Related Accession (Same CDS sequence)	NM_001047878.1
Accession Version	NM_001047878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Rattus norvegicus(Norway rat)
Product	coagulation factor V
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY321333.1. On or before Sep 8, 2006 this sequence version replaced XM_222831.4, XM_001075221.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY321333.1 [ECO:0000332] ##Evidence-Data-END##

ATGCAAAGCT CAGAAGGTCA AGTCCAAGAG AAGCAGGCGG AGATCCGGAG GAGTGACAAA 
AATCATGTCG GGGCCTTTCT ATATGTTCCT CTATATGTCT TTCATGATCT TGAATATAAA 
CAATTTCAAA CCTATCCTTC CTTAGGACTT CTCGGACCAA CTTTATATGC TGAAGTTGGC 
GACACCATCA AGGTTCACTT TAGAAACAAA GCAGACAAAC CTCTAAGCAT CCACCCTCAA 
GGAATTAGAT ACAGCAAATT TTCAGAAGCT CAGCTGTTTG CGGAGGAGGA AATGGACTTT 
GCAGCACCAT TCATCCTCCT TCGATGTTTA TTTGACCCTC CCTCACTCAG TCAGCAAGCA 
TTGGTTCTTC TTCCAAAACG GAAGACTGGT TTCTACGTCA CCCCTCTACT CCTGGAGGGA 
GTGCCCGGTG TTCATAGGTT GTCAATGGGT CTTTACACAA TGAAGCGAGG CATCTTCACC 
CTGGGACAGA CTGGCTTCAT GTGCCCAAGG AGTAGGCTGC ATGCAGGAGC TGGATGTGGA 
GACCGCAGGG GTGGGGAAGA GCCAGAAAGA GCTAGCGCAT CACCCTTTCT CCACACTGGC 
GAGTGGTGGA AAACTCTGGA TGTCTTTGTG GCGGTCACTT CTCCAGGGGC TTCTTACCTG 
GACCACACAT CCCCGGCTGA GAGGAAGGAC GATGCTGTGG CTGCTGGAGA GGAATACACC 
TATGAATGGT TCATCGGTGA GGACAGCGGG CCCACACCTG ATGACCCACC ATGCCTCACC 
CACATCTACT ACTCCTATGA AAACCTGACC CAGGATTTCA ACTCAGGCCT GATTGGGCCT 
CTGCTCATCT GCAAGGAAGG CACCCTGACT GAGGATGGGA CTCAGAAGAT GTTTGACAAG 
CAACACGTGC TTCTGTTTGC TGTATTTGAT GAAAGCAAGA GCTGGAGCCA GTCACCAGCC 
CTCATGTACA CAGTCAACGG CTTTGTGAAT AGGACAATGC CAGATGTAAC AGTCTGTGCC 
TACGATCATG TCAGCTGGCA TCTGATTGGG ATGAGCTCGG GACCAGAATT GTTTTCTATT 
CACTTCAACG GCCAAGTCCT AGAGCAGAAC CAACATAAAG TGTCCACCGT GACTCTGCTG 
CAGACTGTAG GCATCTTTAT CCGACCAATA GCTTTAAATA CTGTGCAAGG TTACATAGCA 
TCCCGTGGTG AGGATCTCCT TGTCCCTTGG GCAACCAGAG CTGCTGGGAT GCAGGCTTAT 
ATTGACATTA AAAACTGCCC AAAGAAAACC AGGAGCTCCA AGACGCTCAC CCGAGAGCAG 
CGGCGGCACA TGAAGAGGTG GGAGTACTTC ATCGCCGCAG AGGAAGTCAT TTGGAACTAC 
GCACCTGTGA TACCCGCAAA CATGGACAAG CATTCTTATC CACTAAGTCC CATGGGAATG 
TGGCCTGATG ATAGTAACAG GAACAATGGC AGTAGAATGC TAGCTACTGT TAACCCTTGT 
AGTAGGTGTT TGCTAAGGCT GTCACATCAG TTCTACAAGC TAGGTGCTAT TCTTGTGTTC 
TTGTATCTAA GGGAAGGTGC CAGAAAATCG GAAGCTCTAG TAGTTCGCCA TATGCCATTT 
CTAAGGGTAG CAGACATGGA GCAGGAGGTC GTGTTTGCTA TGTTTGATGA GAACAAGAGC 
TGGTACATCG AGGACAACAT CAACAAGTTT TGTGAGAATC CCGATGAGGT GAAGCGTGAT 
GACCCCAAGT TTTATGAATC AAACATCATG AACACTATCA ATGGGTACGT GCCTGAGAGC 
ATATCCACCC TAGGATTCTG TTTTGATGAT GCCGTACAGT GGCACTTCTG CAGTGTGGGA 
ACTCATGATG ACATTCTGAC CGTCCACTTC ACTGGGCACT CATTCATCTA TGGGAGGAGG 
CACGAGGACA CCTTGACCCT GTTCCCCATG AGTGGTGAAT CTGTGACTGT CGTGATGGAT 
AATATTGGAA CTTGGATGTT GACCACCATG AGTTCCAATC CAAGACGCAG AAACCTAAGA 
TTGAGATTCA GGGATGTTAA GTGTAACCGG AATGATGATG ACGATGAAGA CTCATATGAG 
ATTTATCAAC CTCTTGAACC TACATCCATG ACAACTCGGA AAATTCATGA TTCTGTGGAA 
AATGACTTTG GCATAGAAAA CGAAGATGAT GATTACCAGT ACGAACTGGC GTCAACACTA 
GGAATTAGGT CATTCAGAAA GTCATTGTTG AATCCGAAGG AAGACGAGTT CAATCTCACT 
GCTCTCGCTC TAGAAAACAG CTCTGAATTC ATATCTCTAA GCACAGACGG AGTTGTTGAC 
TCAAAATCCT CAAGAAACCT TAGTAAGATC ACCAATAATA ACCTCAACGA CTCTCAAAGA 
ACACTTTCTG GCTCAGGAGC CACCATAGCT GGTATCCTAC TTGGAAACCT TACTGGCTTA 
GGTAAGAACT CCGTCCTCAA CCCTTCAACA GAATATCATT CCAGCTCATA TTATGAAAAT 
GATATGGAAG ACCCACAGTC GAACATCACA GTGGTATACC TACTTCCTCT TGGTGCAAAA 
GGATCAGGGA GTCGAGAACA GACTAAACCT AAAACAATCA AGACAGGAAG ACCCCACAGG 
ATGAAGCACA GGTTCTCCTG GATGAAAGCT CCAGCCGGAA AAACTGGGAG GCATTCAAAT 
CCAAAGAATA CTTCTTCCAG AATGAAGTCT GAGGAGGACA TTCCTAGTGA TTTGTTACTC 
TTAAAACAAA AGGTCGCATC CAAACTTTTG AACAGACAGT GGCATATGGC TTCTGAAAAG 
GGTAGTTATG AAATAATACC AGCAAATGGT GAAAACACAG ATATTGATAA GCTGACAAAC 
AGTCCTCAAA ATCAGAATAT CTCAACGCCT TGGGGAGCTA GCACCTCTCG CATAAACACG 
ACAGGGAAGC CAAGTAACCT CCCAACATTT TCTAGATTTA GGCATAAATC TCCACATGTA 
AGACAGGAGG AGGAAAGCGG TGACTTTAAG AAAAGACAGT TGTTCATCAG GACACGGAAG 
AAGAAGAAGA ACAGGAAAAG ACTACTCAAT CATTCATTAC TACTCCACAA ATCCAATGAA 
ACAGCTCTTT CCACAGATCT GAATCAGACC TCCCCTCCAG TGAGTACTGA CAGGTCACTT 
CCTGACTATA ATCAGAACCC TTCAAATGAT ACTGAGCAGA TGAGCTCTTC TTTAGACCTT 
TTTCAGTCAG TGCCACCAGA GGAACACTCT CCAACATTTC CTACCCAAGA TCCTAATCAA 
ACACACTCTA CCACAGATCC TAGCTACAGA TCCTCTCCTC CAGAGCCCAG TCAAGGGATC 
GATTATGACC TAAGCCATGA ATTTTATTCT GATGACATTA GTCAAACATC TTTCTTTCCA 
GACCAAAGTC AAAAGTCACC TCTCACTTCA GATGATGGCC AAGCAATCCC TTCCTCAGAC 
TTAAATCTCT TTACCATCTC TCCAGAATTG GATCAAACAA TTATTTACCC AGACCTGGAT 
CAGTTGTTCC TTTCTCCAGA TGACATCCAG AAGACCTCCT CCCAAGACCT GGGTCAGGTG 
ACCCTTTCTC CAGATGAAAA CCAAGAGACC TCCTCCCAAG ACCTGGGTCA GGTGACCCTT 
TCTCCAAATG AAAACCAAGA GACCTCCTCC CAAGACCTGG GTCAGGTGAC CCTTTCTCCA 
GATGACATCC AGGAGACCTC CTCCCAAGAC CTGGGTCAGG TGACCCTTTC TCCAGATGAA 
AACCAAGAGA CCTCCTCCCC AGACCTGGGT CAGGTGCCCC TTACCCCAGA TGACAACCAG 
AAGACTTCCC CAGACCTAGG TCAGGTGCTC CTTTCTCTAG ATGACAAGCA GAAGACTTAC 
TTCCTAGACC CAGGTCAGGT ACCCATCTCC TCAGACCAAA GCTGGGAGAC CTCCTCCACT 
GACCTTAGCC TACTGACTCT CTCTCCGAAA TTTGGTCAGA CAGTCATTTC CCCAGACTTG 
GATAAGATGC CCCTCTCTTC AGACAACAGT CAAGTTACCC TTTCCCCAGA CCTCAGCCTC 
TTGACCCTCT CACCAGATTT TAATGAGATA ATACTATCCC CAGACATTGA TCAGGTGACC 
CTCTCTCCAG ACCTCATCCA GACAAGCCCT GCTCTTAATC ACAGGCACAA AACATCCTCT 
GCAGACCCTG GTCAAGCATC CTACCCCCCA GATTCTGGTC AGTCTTCACC TCTTCCAGAA 
CTGAATCAGA CTCTTCCTCA TCCAGACCTC ATTCACATGC AGCCTCCTTT ACTATCTCCC 
ACACCTAATG ACACTTCTTT GTCAAAGACA TTTAACCCCC TTGTTGTAGT AGGTCTCAGT 
AGAGTTGATG GAGACGATGT TGAGATGATT CCAAGTGAGG AGCTAGAGAG CATTGACGAA 
GATTATCCTG AGGATGACTA TGTAACCTAC AATGACCCCT ACAAAACAGA CACCAGGGCA 
AATGTCAACT CCTCCAGAAA CCCTGACACT ATTGCAGCAT GGTATCTCCG TAGCTTCGGT 
GGGAACAAAA AATTCTACTA TATTGCTGCT GAAGAAATAT CCTGGGATTA TTCAAAATTT 
GCACCAAGTG AAATGGACAA TGAAGAAACA GACAACACTC CAAAGGACAC CACATACAAG 
AAAGTTGTTT TCAGAAAATA CCTTGATAGT ACTTTCACAA GCCGTGATCC TCAGGGGGAA 
TATGAGGAGC ACCTTGGCAT TCTTGGCCCT GTAATACGTG CTGAAGTGGA TGATGTGATC 
CAAGTTCGAT TTAAAAATTT AGCATCCAGA CCATATTCTC TTCATGCCCA TGGACTTTCC 
TATGAAAAGT CCTCAGAGGG AAAGAATTAC GAAGATGACT CTCCTAAATG GTTTCAGGAA 
GATGATGCTG TCCAGCCCAA TAGCAGTTAC ACATATGTAT GGCATGCCAC TGAGCGCGCG 
GGACCAGAGA ACCCCGGTTC TGCCTGCCGG GCTTGGGTCT ACTATTCTGC AGTGGATGTG 
GAGCGAGACA TCCACTCAGG CTTGATCGGC CCCCTTCTGA TCTGCCGGAA AGGAACACTT 
GACAGGGCGA GCAACCTGCC TCTGGACATG AGAGAGTTTG TCTTGCTCTT CATGGTCTTT 
GATGAGAAGA AGAGCTGGTA CTATGAAAAG TCCAAAGGGT CATGGAGAAT TGAATCTCCA 
GAAGCGAAAA ATTCGCACGA GTTTTACGCA ATTAATGGGA TGATTTACAA CCTGCCCGGC 
CTGAGAATGT ATGAGCAAGA GTGGGTGAGG CTGTACCTGC TGAACATGGG CGGCCCCCAA 
GATATTCACG TGGTTCACTT CCATGGCCAG ATCCTGCTGG ATAATAGGAC CAAACAGCAC 
CACTTAGGGG TCTGGCCCCT CTTGCCTGAG TGCAAGATGC CAATGGGTCT AAGTACTGGT 
GCCATATCTG ACTCACAGAT CAAGGCTTCA GAATATCTGA GCTGCCTTGG AGGACATGCT 
AGAGGACTGA CAGACCCTGT CTGGTACTCC ATGAGCCCAG ACAAGATTCC TAGATCATTC 
CTCATTCCCA GGCTCCTCCC CAAAGAAAAA TATTCAAATG CAGAGAGCCT GTCTGTCATC 
ATTGTGCAAA CCACCTTGAG TCTGTTTCTC TTGGTACTTT ATTCTTTTGC TGGCAATTCA 
GATGCCTCTA CGATAAAAGA GAATCGATTT GACCCACCTA TTGTGGCTAG ATACATTAGG 
ATACATCCCA CGAAATCCTA TAACAGACCC ACCCTTCGGT TGGAGCTGTT GGGCTGTGAG 
GTGAACGGAT GCTCCACACC ACTGGGCCTG GAAGATGGAA GGATACAAAA CAAGCAAATT 
ACTGCATCTT CATTTAAAAA GTCATGGTGG GGAAGCTACT GGGAGCCTTC CCTTGCCCGC 
CTGAATGCCC AGGGCCGAGT GAATGCCTGG CAAGCCAAGG CAAACAACAA CAAGCAGTGG 
TTACAAATTG ATCTGCTCAA AATCAAGAAG GTAACGGCCA TCGTAACTCA GGGTTGCAAG 
TCTCTGTCCT CTGAGATGTA TGTGAAGAGC TACAGCATCC TGTACAGTGA CCAGGGTGTC 
TCCTGGAAAC CCTACCGGCA GAAATCCTCC ATGGTGGACA AGATTTTTGA AGGGAATAGC 
AATACCAAAG GGCATATGAA GAACTTTTTC AACCCACCTA TTATTTCCAG ATTTATCCGA 
ATCATTCCTA AAACATGGAA CCAAAGTATT GCACTTCGCC TGGAACTCTT CGGCTGTGAC 
ATTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041343.1
CDS	1..6309
Translation	MQSSEGQVQEKQAEIRRSDKNHVGAFLYVPLYVFHDLEYKQFQTYPSLGLLGPTLYAEVGDTIKVHFRNKADKPLSIHPQGIRYSKFSEAQLFAEEEMDFAAPFILLRCLFDPPSLSQQALVLLPKRKTGFYVTPLLLEGVPGVHRLSMGLYTMKRGIFTLGQTGFMCPRSRLHAGAGCGDRRGGEEPERASASPFLHTGEWWKTLDVFVAVTSPGASYLDHTSPAERKDDAVAAGEEYTYEWFIGEDSGPTPDDPPCLTHIYYSYENLTQDFNSGLIGPLLICKEGTLTEDGTQKMFDKQHVLLFAVFDESKSWSQSPALMYTVNGFVNRTMPDVTVCAYDHVSWHLIGMSSGPELFSIHFNGQVLEQNQHKVSTVTLLQTVGIFIRPIALNTVQGYIASRGEDLLVPWATRAAGMQAYIDIKNCPKKTRSSKTLTREQRRHMKRWEYFIAAEEVIWNYAPVIPANMDKHSYPLSPMGMWPDDSNRNNGSRMLATVNPCSRCLLRLSHQFYKLGAILVFLYLREGARKSEALVVRHMPFLRVADMEQEVVFAMFDENKSWYIEDNINKFCENPDEVKRDDPKFYESNIMNTINGYVPESISTLGFCFDDAVQWHFCSVGTHDDILTVHFTGHSFIYGRRHEDTLTLFPMSGESVTVVMDNIGTWMLTTMSSNPRRRNLRLRFRDVKCNRNDDDDEDSYEIYQPLEPTSMTTRKIHDSVENDFGIENEDDDYQYELASTLGIRSFRKSLLNPKEDEFNLTALALENSSEFISLSTDGVVDSKSSRNLSKITNNNLNDSQRTLSGSGATIAGILLGNLTGLGKNSVLNPSTEYHSSSYYENDMEDPQSNITVVYLLPLGAKGSGSREQTKPKTIKTGRPHRMKHRFSWMKAPAGKTGRHSNPKNTSSRMKSEEDIPSDLLLLKQKVASKLLNRQWHMASEKGSYEIIPANGENTDIDKLTNSPQNQNISTPWGASTSRINTTGKPSNLPTFSRFRHKSPHVRQEEESGDFKKRQLFIRTRKKKKNRKRLLNHSLLLHKSNETALSTDLNQTSPPVSTDRSLPDYNQNPSNDTEQMSSSLDLFQSVPPEEHSPTFPTQDPNQTHSTTDPSYRSSPPEPSQGIDYDLSHEFYSDDISQTSFFPDQSQKSPLTSDDGQAIPSSDLNLFTISPELDQTIIYPDLDQLFLSPDDIQKTSSQDLGQVTLSPDENQETSSQDLGQVTLSPNENQETSSQDLGQVTLSPDDIQETSSQDLGQVTLSPDENQETSSPDLGQVPLTPDDNQKTSPDLGQVLLSLDDKQKTYFLDPGQVPISSDQSWETSSTDLSLLTLSPKFGQTVISPDLDKMPLSSDNSQVTLSPDLSLLTLSPDFNEIILSPDIDQVTLSPDLIQTSPALNHRHKTSSADPGQASYPPDSGQSSPLPELNQTLPHPDLIHMQPPLLSPTPNDTSLSKTFNPLVVVGLSRVDGDDVEMIPSEELESIDEDYPEDDYVTYNDPYKTDTRANVNSSRNPDTIAAWYLRSFGGNKKFYYIAAEEISWDYSKFAPSEMDNEETDNTPKDTTYKKVVFRKYLDSTFTSRDPQGEYEEHLGILGPVIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKNYEDDSPKWFQEDDAVQPNSSYTYVWHATERAGPENPGSACRAWVYYSAVDVERDIHSGLIGPLLICRKGTLDRASNLPLDMREFVLLFMVFDEKKSWYYEKSKGSWRIESPEAKNSHEFYAINGMIYNLPGLRMYEQEWVRLYLLNMGGPQDIHVVHFHGQILLDNRTKQHHLGVWPLLPECKMPMGLSTGAISDSQIKASEYLSCLGGHARGLTDPVWYSMSPDKIPRSFLIPRLLPKEKYSNAESLSVIIVQTTLSLFLLVLYSFAGNSDASTIKENRFDPPIVARYIRIHPTKSYNRPTLRLELLGCEVNGCSTPLGLEDGRIQNKQITASSFKKSWWGSYWEPSLARLNAQGRVNAWQAKANNNKQWLQIDLLKIKKVTAIVTQGCKSLSSEMYVKSYSILYSDQGVSWKPYRQKSSMVDKIFEGNSNTKGHMKNFFNPPIISRFIRIIPKTWNQSIALRLELFGCDIY

Target ORF information:

RefSeq Version	NM_001047878.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus coagulation factor V (F5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047878.1

ORF Insert Sequence:

ATGCAAAGCT CAGAAGGTCA AGTCCAAGAG AAGCAGGCGG AGATCCGGAG GAGTGACAAA 
AATCATGTCG GGGCCTTTCT ATATGTTCCT CTATATGTCT TTCATGATCT TGAATATAAA 
CAATTTCAAA CCTATCCTTC CTTAGGACTT CTCGGACCAA CTTTATATGC TGAAGTTGGC 
GACACCATCA AGGTTCACTT TAGAAACAAA GCAGACAAAC CTCTAAGCAT CCACCCTCAA 
GGAATTAGAT ACAGCAAATT TTCAGAAGCT CAGCTGTTTG CGGAGGAGGA AATGGACTTT 
GCAGCACCAT TCATCCTCCT TCGATGTTTA TTTGACCCTC CCTCACTCAG TCAGCAAGCA 
TTGGTTCTTC TTCCAAAACG GAAGACTGGT TTCTACGTCA CCCCTCTACT CCTGGAGGGA 
GTGCCCGGTG TTCATAGGTT GTCAATGGGT CTTTACACAA TGAAGCGAGG CATCTTCACC 
CTGGGACAGA CTGGCTTCAT GTGCCCAAGG AGTAGGCTGC ATGCAGGAGC TGGATGTGGA 
GACCGCAGGG GTGGGGAAGA GCCAGAAAGA GCTAGCGCAT CACCCTTTCT CCACACTGGC 
GAGTGGTGGA AAACTCTGGA TGTCTTTGTG GCGGTCACTT CTCCAGGGGC TTCTTACCTG 
GACCACACAT CCCCGGCTGA GAGGAAGGAC GATGCTGTGG CTGCTGGAGA GGAATACACC 
TATGAATGGT TCATCGGTGA GGACAGCGGG CCCACACCTG ATGACCCACC ATGCCTCACC 
CACATCTACT ACTCCTATGA AAACCTGACC CAGGATTTCA ACTCAGGCCT GATTGGGCCT 
CTGCTCATCT GCAAGGAAGG CACCCTGACT GAGGATGGGA CTCAGAAGAT GTTTGACAAG 
CAACACGTGC TTCTGTTTGC TGTATTTGAT GAAAGCAAGA GCTGGAGCCA GTCACCAGCC 
CTCATGTACA CAGTCAACGG CTTTGTGAAT AGGACAATGC CAGATGTAAC AGTCTGTGCC 
TACGATCATG TCAGCTGGCA TCTGATTGGG ATGAGCTCGG GACCAGAATT GTTTTCTATT 
CACTTCAACG GCCAAGTCCT AGAGCAGAAC CAACATAAAG TGTCCACCGT GACTCTGCTG 
CAGACTGTAG GCATCTTTAT CCGACCAATA GCTTTAAATA CTGTGCAAGG TTACATAGCA 
TCCCGTGGTG AGGATCTCCT TGTCCCTTGG GCAACCAGAG CTGCTGGGAT GCAGGCTTAT 
ATTGACATTA AAAACTGCCC AAAGAAAACC AGGAGCTCCA AGACGCTCAC CCGAGAGCAG 
CGGCGGCACA TGAAGAGGTG GGAGTACTTC ATCGCCGCAG AGGAAGTCAT TTGGAACTAC 
GCACCTGTGA TACCCGCAAA CATGGACAAG CATTCTTATC CACTAAGTCC CATGGGAATG 
TGGCCTGATG ATAGTAACAG GAACAATGGC AGTAGAATGC TAGCTACTGT TAACCCTTGT 
AGTAGGTGTT TGCTAAGGCT GTCACATCAG TTCTACAAGC TAGGTGCTAT TCTTGTGTTC 
TTGTATCTAA GGGAAGGTGC CAGAAAATCG GAAGCTCTAG TAGTTCGCCA TATGCCATTT 
CTAAGGGTAG CAGACATGGA GCAGGAGGTC GTGTTTGCTA TGTTTGATGA GAACAAGAGC 
TGGTACATCG AGGACAACAT CAACAAGTTT TGTGAGAATC CCGATGAGGT GAAGCGTGAT 
GACCCCAAGT TTTATGAATC AAACATCATG AACACTATCA ATGGGTACGT GCCTGAGAGC 
ATATCCACCC TAGGATTCTG TTTTGATGAT GCCGTACAGT GGCACTTCTG CAGTGTGGGA 
ACTCATGATG ACATTCTGAC CGTCCACTTC ACTGGGCACT CATTCATCTA TGGGAGGAGG 
CACGAGGACA CCTTGACCCT GTTCCCCATG AGTGGTGAAT CTGTGACTGT CGTGATGGAT 
AATATTGGAA CTTGGATGTT GACCACCATG AGTTCCAATC CAAGACGCAG AAACCTAAGA 
TTGAGATTCA GGGATGTTAA GTGTAACCGG AATGATGATG ACGATGAAGA CTCATATGAG 
ATTTATCAAC CTCTTGAACC TACATCCATG ACAACTCGGA AAATTCATGA TTCTGTGGAA 
AATGACTTTG GCATAGAAAA CGAAGATGAT GATTACCAGT ACGAACTGGC GTCAACACTA 
GGAATTAGGT CATTCAGAAA GTCATTGTTG AATCCGAAGG AAGACGAGTT CAATCTCACT 
GCTCTCGCTC TAGAAAACAG CTCTGAATTC ATATCTCTAA GCACAGACGG AGTTGTTGAC 
TCAAAATCCT CAAGAAACCT TAGTAAGATC ACCAATAATA ACCTCAACGA CTCTCAAAGA 
ACACTTTCTG GCTCAGGAGC CACCATAGCT GGTATCCTAC TTGGAAACCT TACTGGCTTA 
GGTAAGAACT CCGTCCTCAA CCCTTCAACA GAATATCATT CCAGCTCATA TTATGAAAAT 
GATATGGAAG ACCCACAGTC GAACATCACA GTGGTATACC TACTTCCTCT TGGTGCAAAA 
GGATCAGGGA GTCGAGAACA GACTAAACCT AAAACAATCA AGACAGGAAG ACCCCACAGG 
ATGAAGCACA GGTTCTCCTG GATGAAAGCT CCAGCCGGAA AAACTGGGAG GCATTCAAAT 
CCAAAGAATA CTTCTTCCAG AATGAAGTCT GAGGAGGACA TTCCTAGTGA TTTGTTACTC 
TTAAAACAAA AGGTCGCATC CAAACTTTTG AACAGACAGT GGCATATGGC TTCTGAAAAG 
GGTAGTTATG AAATAATACC AGCAAATGGT GAAAACACAG ATATTGATAA GCTGACAAAC 
AGTCCTCAAA ATCAGAATAT CTCAACGCCT TGGGGAGCTA GCACCTCTCG CATAAACACG 
ACAGGGAAGC CAAGTAACCT CCCAACATTT TCTAGATTTA GGCATAAATC TCCACATGTA 
AGACAGGAGG AGGAAAGCGG TGACTTTAAG AAAAGACAGT TGTTCATCAG GACACGGAAG 
AAGAAGAAGA ACAGGAAAAG ACTACTCAAT CATTCATTAC TACTCCACAA ATCCAATGAA 
ACAGCTCTTT CCACAGATCT GAATCAGACC TCCCCTCCAG TGAGTACTGA CAGGTCACTT 
CCTGACTATA ATCAGAACCC TTCAAATGAT ACTGAGCAGA TGAGCTCTTC TTTAGACCTT 
TTTCAGTCAG TGCCACCAGA GGAACACTCT CCAACATTTC CTACCCAAGA TCCTAATCAA 
ACACACTCTA CCACAGATCC TAGCTACAGA TCCTCTCCTC CAGAGCCCAG TCAAGGGATC 
GATTATGACC TAAGCCATGA ATTTTATTCT GATGACATTA GTCAAACATC TTTCTTTCCA 
GACCAAAGTC AAAAGTCACC TCTCACTTCA GATGATGGCC AAGCAATCCC TTCCTCAGAC 
TTAAATCTCT TTACCATCTC TCCAGAATTG GATCAAACAA TTATTTACCC AGACCTGGAT 
CAGTTGTTCC TTTCTCCAGA TGACATCCAG AAGACCTCCT CCCAAGACCT GGGTCAGGTG 
ACCCTTTCTC CAGATGAAAA CCAAGAGACC TCCTCCCAAG ACCTGGGTCA GGTGACCCTT 
TCTCCAAATG AAAACCAAGA GACCTCCTCC CAAGACCTGG GTCAGGTGAC CCTTTCTCCA 
GATGACATCC AGGAGACCTC CTCCCAAGAC CTGGGTCAGG TGACCCTTTC TCCAGATGAA 
AACCAAGAGA CCTCCTCCCC AGACCTGGGT CAGGTGCCCC TTACCCCAGA TGACAACCAG 
AAGACTTCCC CAGACCTAGG TCAGGTGCTC CTTTCTCTAG ATGACAAGCA GAAGACTTAC 
TTCCTAGACC CAGGTCAGGT ACCCATCTCC TCAGACCAAA GCTGGGAGAC CTCCTCCACT 
GACCTTAGCC TACTGACTCT CTCTCCGAAA TTTGGTCAGA CAGTCATTTC CCCAGACTTG 
GATAAGATGC CCCTCTCTTC AGACAACAGT CAAGTTACCC TTTCCCCAGA CCTCAGCCTC 
TTGACCCTCT CACCAGATTT TAATGAGATA ATACTATCCC CAGACATTGA TCAGGTGACC 
CTCTCTCCAG ACCTCATCCA GACAAGCCCT GCTCTTAATC ACAGGCACAA AACATCCTCT 
GCAGACCCTG GTCAAGCATC CTACCCCCCA GATTCTGGTC AGTCTTCACC TCTTCCAGAA 
CTGAATCAGA CTCTTCCTCA TCCAGACCTC ATTCACATGC AGCCTCCTTT ACTATCTCCC 
ACACCTAATG ACACTTCTTT GTCAAAGACA TTTAACCCCC TTGTTGTAGT AGGTCTCAGT 
AGAGTTGATG GAGACGATGT TGAGATGATT CCAAGTGAGG AGCTAGAGAG CATTGACGAA 
GATTATCCTG AGGATGACTA TGTAACCTAC AATGACCCCT ACAAAACAGA CACCAGGGCA 
AATGTCAACT CCTCCAGAAA CCCTGACACT ATTGCAGCAT GGTATCTCCG TAGCTTCGGT 
GGGAACAAAA AATTCTACTA TATTGCTGCT GAAGAAATAT CCTGGGATTA TTCAAAATTT 
GCACCAAGTG AAATGGACAA TGAAGAAACA GACAACACTC CAAAGGACAC CACATACAAG 
AAAGTTGTTT TCAGAAAATA CCTTGATAGT ACTTTCACAA GCCGTGATCC TCAGGGGGAA 
TATGAGGAGC ACCTTGGCAT TCTTGGCCCT GTAATACGTG CTGAAGTGGA TGATGTGATC 
CAAGTTCGAT TTAAAAATTT AGCATCCAGA CCATATTCTC TTCATGCCCA TGGACTTTCC 
TATGAAAAGT CCTCAGAGGG AAAGAATTAC GAAGATGACT CTCCTAAATG GTTTCAGGAA 
GATGATGCTG TCCAGCCCAA TAGCAGTTAC ACATATGTAT GGCATGCCAC TGAGCGCGCG 
GGACCAGAGA ACCCCGGTTC TGCCTGCCGG GCTTGGGTCT ACTATTCTGC AGTGGATGTG 
GAGCGAGACA TCCACTCAGG CTTGATCGGC CCCCTTCTGA TCTGCCGGAA AGGAACACTT 
GACAGGGCGA GCAACCTGCC TCTGGACATG AGAGAGTTTG TCTTGCTCTT CATGGTCTTT 
GATGAGAAGA AGAGCTGGTA CTATGAAAAG TCCAAAGGGT CATGGAGAAT TGAATCTCCA 
GAAGCGAAAA ATTCGCACGA GTTTTACGCA ATTAATGGGA TGATTTACAA CCTGCCCGGC 
CTGAGAATGT ATGAGCAAGA GTGGGTGAGG CTGTACCTGC TGAACATGGG CGGCCCCCAA 
GATATTCACG TGGTTCACTT CCATGGCCAG ATCCTGCTGG ATAATAGGAC CAAACAGCAC 
CACTTAGGGG TCTGGCCCCT CTTGCCTGAG TGCAAGATGC CAATGGGTCT AAGTACTGGT 
GCCATATCTG ACTCACAGAT CAAGGCTTCA GAATATCTGA GCTGCCTTGG AGGACATGCT 
AGAGGACTGA CAGACCCTGT CTGGTACTCC ATGAGCCCAG ACAAGATTCC TAGATCATTC 
CTCATTCCCA GGCTCCTCCC CAAAGAAAAA TATTCAAATG CAGAGAGCCT GTCTGTCATC 
ATTGTGCAAA CCACCTTGAG TCTGTTTCTC TTGGTACTTT ATTCTTTTGC TGGCAATTCA 
GATGCCTCTA CGATAAAAGA GAATCGATTT GACCCACCTA TTGTGGCTAG ATACATTAGG 
ATACATCCCA CGAAATCCTA TAACAGACCC ACCCTTCGGT TGGAGCTGTT GGGCTGTGAG 
GTGAACGGAT GCTCCACACC ACTGGGCCTG GAAGATGGAA GGATACAAAA CAAGCAAATT 
ACTGCATCTT CATTTAAAAA GTCATGGTGG GGAAGCTACT GGGAGCCTTC CCTTGCCCGC 
CTGAATGCCC AGGGCCGAGT GAATGCCTGG CAAGCCAAGG CAAACAACAA CAAGCAGTGG 
TTACAAATTG ATCTGCTCAA AATCAAGAAG GTAACGGCCA TCGTAACTCA GGGTTGCAAG 
TCTCTGTCCT CTGAGATGTA TGTGAAGAGC TACAGCATCC TGTACAGTGA CCAGGGTGTC 
TCCTGGAAAC CCTACCGGCA GAAATCCTCC ATGGTGGACA AGATTTTTGA AGGGAATAGC 
AATACCAAAG GGCATATGAA GAACTTTTTC AACCCACCTA TTATTTCCAG ATTTATCCGA 
ATCATTCCTA AAACATGGAA CCAAAGTATT GCACTTCGCC TGGAACTCTT CGGCTGTGAC 
ATTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The relationship between the levels and function of endothelial progenitor cells and factor V Leiden and protein C deficiency in patients with primary Budd-Chiari syndrome.
European review for medical and pharmacological sciences22(9)2742-2750(2018 05)
Guan ZY,Yu CW,Song T,Gao Y

Association of factor V Leiden, Janus kinase 2, prothrombin, and MTHFR mutations with primary Budd-Chiari syndrome in Egyptian patients.
Journal of gastroenterology and hepatology31(1)235-40(2016 Jan)
El Sebay HM,Safan MA,Daoud AA,Tayel SI,Nouh MA,El Shafie S

Prothrombotic genetic risk factors are associated with an increased risk of liver fibrosis in the general population: The Rotterdam Study.
Journal of hepatology63(6)1459-65(2015 Dec)
Plompen EP,Darwish Murad S,Hansen BE,Loth DW,Schouten JN,Taimr P,Hofman A,Uitterlinden AG,Stricker BH,Janssen HL,Leebeek FW

Plasminogen activator inhibitor-1 4G/5G polymorphism, factor V Leiden, prothrombin mutations and the risk of VTE recurrence.
Thrombosis and haemostasis114(6)1156-64(2015 Nov)
Sundquist K,Wang X,Svensson PJ,Sundquist J,Hedelius A,Larsson Lönn S,Zöller B,Memon AA

How it all starts: Initiation of the clotting cascade.
Critical reviews in biochemistry and molecular biology50(4)326-36(2015)
Smith SA,Travers RJ,Morrissey JH

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F5 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

ORF Insert Sequence:

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