f5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	f5
Entrez Gene ID	327320
Full Name	coagulation factor V
Synonyms	si:bz20i5.3,wu:fd21h06
General protein information	Preferred Names coagulation factor V Names coagulation factor V coagulation factor V (proaccelerin, labile factor)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328531.1	NP_001315460.1	coagulation factor V precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	F5	NP_776304.1
Rattus norvegicus (Norway rat)	F5	NP_001041343.1
Gallus gallus (chicken)	F5	XP_001231901.3
Danio rerio (zebrafish)	f5	NP_001007209.2
Mus musculus (house mouse)	F5	NP_032002.1
Pan troglodytes (chimpanzee)	F5	XP_513984.4
Homo sapiens (human)	F5	NP_000121.2
Macaca mulatta (Rhesus monkey)	F5	XP_001093072.2
Canis lupus familiaris (dog)	F5	XP_005622605.1
Xenopus tropicalis (tropical clawed frog)	f5	XP_002935820.2

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of factor V in zebrafish demonstrates minimal levels needed for early hemostasis.
Weyand AC, Grzegorski SJ, Rost MS, Lavik KI, Ferguson AC, Menegatti M, Richter CE, Asselta R, Duga S, Peyvandi F, Shavit JA
Blood advances3(11)1670-1680(2019 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Systems pharmacology of adiposity reveals inhibition of EP300 as a common therapeutic mechanism of caloric restriction and resveratrol for obesity.
Nishimura Y, Sasagawa S, Ariyoshi M, Ichikawa S, Shimada Y, Kawaguchi K, Kawase R, Yamamoto R, Uehara T, Yanai T, Takata R, Tanaka T
Frontiers in pharmacology6199(2015)

The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
Nesan D, Vijayan MM
PloS one8(11)e80726(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following f5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the f5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa15388	NM_001328531.1 Latest version!	Danio rerio coagulation factor V (f5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

f5 ( NM_001328531.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315460.1 Danio rerio coagulation factor V (f5), mRNA.

Price & Availability↑

CloneID	ODa15388
Clone ID Related Accession (Same CDS sequence)	NM_001328531.1
Accession Version	NM_001328531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573228800000
Organism	Danio rerio(zebrafish)
Product	coagulation factor V precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL590146.2. On Jun 17, 2016 this sequence version replaced XM_005167820.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01009978.1, BC171702.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505378, SAMEA3505392 [ECO:0000348] ##Evidence-Data-END##

ATGAAACTCC GCTCCTGGCC AGGGGCCCTT CATGGTTTGG CCCTCCTGGC ATTCCTGTGT 
CATTGTGCAA CAGCTGTGGA GAGACATTAC TATATTGCAG CTGTTAATAT TAACTGGGAC 
TACACATCTG GACAGCAAAG AACCGGACAA TCATATAAAA AAGTAGTATA CAGGGAATAT 
AATGAGGGAT TCAAACAGCC AAAAGCTCAT CCCTTGTCCT CAGGGTTACT TGGGCCTACA 
TTGCGTGGAC AAGAGGGTGA CACAATCATC GTCACGTTCA GGAACATGGC AGATCATCCT 
TGCAGCCTCC ACCCCCATGG CATTGCTTAC GGGAAGCAGT CAGAGGGGTC TTTATACTTT 
GACAACACGT CACTCCTTGA GAAAAATGAT GATGTAATTC AACCTGGTGA AGAGCACACT 
TACCAGTGGG ACGTGACGTC TGATGTGACC CCTACAGCTG CAGACCCACC ATGCATCACC 
TACACATACT TGTCTCACTT TGACATTGTC CGAGACTACA ATACAGGGTT AATAGGCCCA 
ATGCTGATCT GCAAGAAAGG CACCCTAGAC GACTCAGGAA ATCAGATTCA TTTCCATCAA 
GAGTCTGTGC TGCTGTTTGG AGTATTTGAT GAGAACAAGA GCTGGTACAG CACTGGGGAC 
TCTCCACAGC CTCTGAATGT CAAATACACA ATAAACGGCT ACACAAATGG CTCAGTACCA 
GATCTGGACA TCTGTGCCCA CTCTAAAGTC AGCTGGCACC TGCTGGGAAT GAGCTCAGAG 
CCAGAGCTTT TCTCTGTACA CTTCAATGGG CAAGTCCTTC TGCACGATGG GCACAAAACT 
TCAGCTGTTG GCATTATTAG TGGAACTGCC ACCAGTGCCA GTATGACCGG TGTCCACCCT 
GGCCGCTGGC TCGTTTCCTC GCACATTAGC AAGCACTTGG AAGCTGGTTT GCACGGGTAC 
CTAAATATCA GGAAGTGTGA TGAGTATACG GCGCCAAAAA GACGGCTTAC CATTGAACAG 
AAAAAAGAGA GTCAAGAGTG GACTTACTAT ATGGCAGCAG AAGAGGTCAT CTGGGATTAT 
GCGCCAAATA TGCCAGAAAA CATGGACGGG GATTTCCGGT CAAAATATTT AAAGCAGGGG 
CCTCAGCGGA TAGGTAAAAA ATATAAAAAA GCTGTGTTTA CTCAGTATAA AGATGGCATG 
TTTAAAGAGA GAGCGGAAGA TAAGCAGAGG AAGAGAGAGC TTGGAATTCT TGGCCCAGTG 
ATTAGAGCTC AAATCAGAGA CATCATTAAG ATTGTCTTTA AAAACAAAGC ATCACGTCCC 
TATAGTATCT ATCCCCATGG ACTGACCATA GATAAAGCAG CAGAAGGAGC CAGTTACCCT 
CAGGGAGGGA ACCAAACATA CAGCGTTCAG CCAGGAGAGA CGTACACATA TACATGGAGT 
GTGACTGAGG AAGACGTACC AACGGACAGT GACCCCAGAT GTCTAACCAG GATGTACCAC 
AGTGCAGTGG ACGCTCCTCG AGACATTGCT TCAGGTCTTG TTGGCCCTCT GCTCATCTGT 
AAGAGCCAGT CTCTTAACAA AAAGAATGTC CAGCTAAAAG CTGACAAGGA GCAGCATGCC 
ATGTTTACCG TTTTTGATGA GAACAAGAGC TGGTACCAAG ATGAGAACAT TAACACATAC 
TGCAGTGATC CCAAAAAAGT GAAGAAAGAC GATCCCGAGT TTTACAAGTC AAATGTCATG 
CACACAATCA ATGGTTATGT ATATGAAAGC GGCCAAGAAT TGGGATTTTG TCATGGTGAA 
ATTGTGACTT GGCATGTGTC GAGTGTTGGG GAACAGGATT ACATCCAGAC TGCTACTTTC 
TATGGTCATA CATTTGAGCT AAAAAACAGA GAAGAGGATA TACTCAGTCT ATTTCCTATG 
ACTGGTGAAA CTATCACGAT GAACATGGTT AATATAGGTA TTTGGCTTTT GGCATCATTG 
AACTCGCATG ATTCCACTAA AGGGATGAGG GTGAAATTCA AAGACCTCGA ATGCTTCAGA 
GATTATGTAA TAGAATATGA TTATGAGGAC GGGAAATTCA CTGCATGGAA ACCACCGACT 
ATTAATGAAA TCAAAAAAGA GGAACCGGTC CGTGCAAGAC CTGACGTGGT CGACGAGTAC 
TCTGATTTAT TTGCTGAAAC CCTCAACTTA AGGACGTTCA ACAATGTTAA AGATGAAGTT 
GAGATAATCG ACTTGACATT TCTAGATCAA GATGATGGTT TGTTGCCCAT TGTTGAAGAA 
AAAAGCCTGG GATCAAGCAA CGAGAACTTA CACAATGCTA CTTTACAATC TTTCATTGAG 
ACTCATGGTT TATTAATGGA GGAAGGTGAT TTAGACAAGG GAGAATCTTC TAATAAAGTC 
TTGAATGACA GCACAGACAA GGCATTGCTA GAGACAACAA CTACTTTTGA TTCCAATAGA 
GTTGTCGCAT TAAACAACGA AACGGACAGT ATAATTTTAG ATTTTCCAAT TGTAGAAAGA 
AAGGTTCGTA GTGCACCATC AAAGCCAATG AACGAACCTG AAAGTGTCAC TATGAACTTT 
AAAACAACAG AACATATCAA TTCATCATTA GAGAGAATCA ATGCTATATA TTCCCCAATA 
ACTGAAACAA ACATCAACAC AATGACTGAG ACACACACTG ATTTCAGTAT CACTCCATTT 
GATGGTTCAA CTGGAGAAAT GAACTTCACA CTAGAGGATG ACACCGCACT TCTAAATTCA 
TCTGAATCAG AGCCCCTGCA ATCAAACCAA AACTCCGAAA ACAGAATAGC TTTTCAAGAA 
GAGCTAAATG CAAAAGATGG CACAGATGTT GACAGTAATA ATTCTGTGAA AAATCAGATC 
TTCAAATACA ACGTGCCTAG TGGTGACACT CTGTCCAACA GCTCAAAGAT ACAAGTTGAG 
GAGGATTTTG TGCTTCTGGA CAGTAGTTAT TTTTCAGAAA TGTCAACAAC TATGGAATAT 
GATGTTTCAC AAAAGGACAC TGTAAAAGGT GAATCAAAAG AGACTGCACA AAGTCAAGAA 
CTCAGCAGCA CAAAGAAAAC TTACTCTGGT GAAATAATTT TGGAGAGTCT TCCTGACATA 
ACAAGTGCTT TTAATCTGAA TTCATCTTCA GTTTTGAGAA ACAACAGTTT GGAGAGCAAT 
GAGTCCTCAA ATGAAACTTT GTTCATGTCT TCAAATGCTA CCTTCTCTGA TTCAACAAAT 
GCAACCTCGT CTGATTCCTC AACAGCAACC TTCGCTGATT TCTCAAACAC AACCTTCTCA 
AACGCAACCT TCTCTGATTT CTCAAATAGG ATTTCTCAAA TGTCTGATTC CTCAAATGCA 
ACCTTGTCTG ATTCCTCAAA TGCAACCTTG TCTGATTCCT CAAATGCAAC ATTGTCTGAT 
TCCTCAAATG CAACATTGTC TGATTCCTCA AATGCAACAT TGTCTAATTC CTCAAATGCA 
ACCTTCTCTG ATTCCTCAAA CAAAACCTTC TCTGATTCCT CAAATGCAAC CTTGTTTGGT 
GTTTCATATT CCTCAAACAC AACCTTGTCT GACTTCAGTC TTGAATCTGA AATGACAGAG 
TATATACTGT CATCTGCTAA TGATACAATA AAATCTCACA GTGAAGTTGT AAGCAACACT 
TCACAGCTTT CCTCTTCAGA AAGCACAGAG AATATATCGT TGTTGTATGG CTCTCTCAAT 
GCTTCATCCA TGAAGAACGA CTCAGAAAGT GAAAGCGAAG AAGAAGTGGT TATCTACCTA 
AATAAAAATC ACAGTGAGGC AATCCTCACA TCTCATCTCG ACCAAAAAGA AGAGCACTGG 
GGTTATGAAA GCAAACACGA GCTGGTTCAT AAGGAACTCC CAGATCACAT GAACAAATAT 
GTCAAAGATA AGTCTGCAGC AAACTCGAAC AAGCCAAAGA TCGAAAAGGA AAAGAAAAAA 
GTTTACCAAA GAGTAAAGCC AAAAAAGGGG TACGGGATGA AGACGAAGAA AAGTAAAGAC 
TACAAACCGC AGCCGCGTTC GTCATTTTCT CCAAGAGGTT TTGGACCATC TGTGTTAACT 
CCAAGGGGAA GCAGACCTGT CTCTAGCGAG GATGAACTGA CGGAGAAACC TATTGTCATA 
GGAGTGCCAA GACGGGATTT CAATGATTAT GAGTTATATA TTCCAAAACA AGACCAGGAA 
GCAGATTTTG ATGGCTTGCT TGATCACCCA GAAGAATATG AATATGTAGA GTATAAAGAC 
CCTTACAGCA AAACAGCAGA TGTTCAAGCT CTTGACGCAA CATCCCAGCA TCTCTTAAAA 
ATGGCTGGAG ACAAGAATAC CAGGACCTAT TTCATCTCGG TTGAAGAAGA AGAATGGGAT 
TATGCTGGCT ATGGGCAGAG GCGACTTGAT AAAACAGCTC AAAATGAGAG GCCCACGGTT 
TTCAGAAAAG TGGTGTTCCG AAGGTACCTG GACAGCACCT TTAGCATCCG AGACATCCGA 
GGAGAAATGG ACGAACACCT GGGAATTCTT GGTCCGCTCA TTAAAGCGGA AGTTGATCAG 
ACCGTTATGG TATTTTTCAG GAACCGTGCA AGTCGTCCAT ACTCTTTGCA TGCAAATGGA 
GTAAAATACT TAAAACAAAT GGAAGGCCTG AGTTATGATG ATGAATCTCC ATACTGGTAC 
AAACAGGACG ATGCTGTTCC ACCCAATGGC ACCTTTACCT ACATGTGGAC CATAAATCCC 
AAATCTGGAC CTCAAAATAA TGAATCTGAC TGCCGAACTT GGACCTACTA CTCTGCAGTG 
AATCCTGAGA GAGATATAAA CTCAGGGTTG ATTGGGCCAC TTCTGGTGTG CAGGAAGGGT 
ACACTTGATA AAAAGCCTGA AGACAGAAGA GAGTTTGTCC TGCTTTTCAT GACATTTGAT 
GAAAATAAAA GCTGGCTCTA TGAAGAGAAT CGGCAGAGGA TTGAAAGGAA AAACAGAAGA 
GTGGTCATGG ATCCAAATTT CCAAGATAAT TTAAAGTTTG ATGCTATCAA TGGTATCATC 
TACAGTCTCA AAGGACTTCG AATGTACACA AATCAGCTGG CGAAGTGGCA TCTGATCAAC 
ATGGGCTCTC CTAAAGACCT CCACAGTGTT CACTTCCATG GACAAACATT CATAAATAAA 
GAATTAAAGG ACCACCGTCA GGGCGTTTAC CCGCTTTTAC CAGGTGGATT TGCCACGCTG 
GAAATGCTGC CTTCAAAGCC TGGTCTCTGG CAGCTGGAGT CTGAAGTCGG ACTCTCACAG 
CAGAGGGGAA TGCAAACGCT ATTTCTTGTG TTGGATAATG TCTGTGATCA CCCTCTTGGT 
CTCATCTCTG GGACTGTGCA AGATGAGCAA ATAACAGCAT CTGACACTAG AGGACAATGG 
TATCCTCATT TGGCCAGACT TCATAATACA GGGAAATACA ATGCATGGAG TACATCGTCA 
GAGCCAGGAC AGTACTTACA GGTGGACTTC CAGAGGCCGG TGGTGATTAG TAAAGTCGCC 
ACACAAGGAG CCAAACAATT TTTGACACAC AACTTTGTGT TGAACTACAC CATTTCCTAC 
AGCACTGACA AAAAGAAGTG GATCTACTAC AAGGGAGACA GTGATGCTGT CAGAAAGACT 
TTTGAAGGAA ATGCTGAAGC TTATGAGACG AAGGAAAATA TATTTTTCCC ACCTCTGATT 
GGTCGATATG TGAGACTGCA TCCTTTACAT TCTTACAACT TCCCGACTGT CCGTTTGGAG 
TATTATGGTT GTGAGCTTGA TGGCTGCTCT GTGCCATTAG GAATGGAAAA AGGTCTTATT 
GATGACAGTA AAATCACTGC CAGTTCTGTG GCATCCAACT GGTACTCCGG ACAGTGGCAT 
CCTTGGTATG CTCGCCTAAA TAAACAAGGA ACCGCCAATG CATGGCAAGC AAAGAACAAT 
GACATTCAGC CTTGGATCCA GGTGGAACTC AAAGAAGTAA AGAAGATAAC TGGAATTGTA 
ACACAGGGAG CCAAATCAAT GGGCAATGAA ATGTTTGTGA GATCTTATAT TTTGGAATAT 
AGTGAAGATG GCAGGCGATG GATGAAGTAC ACAGATGATG ATGATTATGA GCAAAAGCTA 
TTTCAAGGCA ACACAGATAA CAATGGCCAA ATAAAGAACT ACATCTACCC GCCGATATTT 
TCCAGATTCA TCAGAATAAT ACCAAAACAA TGGCAGAAAT CCGTCACCAT GAGAATTGAG 
CTGCTGGGTT GTGATTTCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315460.1
CDS	56..6319
Translation	MKLRSWPGALHGLALLAFLCHCATAVERHYYIAAVNINWDYTSGQQRTGQSYKKVVYREYNEGFKQPKAHPLSSGLLGPTLRGQEGDTIIVTFRNMADHPCSLHPHGIAYGKQSEGSLYFDNTSLLEKNDDVIQPGEEHTYQWDVTSDVTPTAADPPCITYTYLSHFDIVRDYNTGLIGPMLICKKGTLDDSGNQIHFHQESVLLFGVFDENKSWYSTGDSPQPLNVKYTINGYTNGSVPDLDICAHSKVSWHLLGMSSEPELFSVHFNGQVLLHDGHKTSAVGIISGTATSASMTGVHPGRWLVSSHISKHLEAGLHGYLNIRKCDEYTAPKRRLTIEQKKESQEWTYYMAAEEVIWDYAPNMPENMDGDFRSKYLKQGPQRIGKKYKKAVFTQYKDGMFKERAEDKQRKRELGILGPVIRAQIRDIIKIVFKNKASRPYSIYPHGLTIDKAAEGASYPQGGNQTYSVQPGETYTYTWSVTEEDVPTDSDPRCLTRMYHSAVDAPRDIASGLVGPLLICKSQSLNKKNVQLKADKEQHAMFTVFDENKSWYQDENINTYCSDPKKVKKDDPEFYKSNVMHTINGYVYESGQELGFCHGEIVTWHVSSVGEQDYIQTATFYGHTFELKNREEDILSLFPMTGETITMNMVNIGIWLLASLNSHDSTKGMRVKFKDLECFRDYVIEYDYEDGKFTAWKPPTINEIKKEEPVRARPDVVDEYSDLFAETLNLRTFNNVKDEVEIIDLTFLDQDDGLLPIVEEKSLGSSNENLHNATLQSFIETHGLLMEEGDLDKGESSNKVLNDSTDKALLETTTTFDSNRVVALNNETDSIILDFPIVERKVRSAPSKPMNEPESVTMNFKTTEHINSSLERINAIYSPITETNINTMTETHTDFSITPFDGSTGEMNFTLEDDTALLNSSESEPLQSNQNSENRIAFQEELNAKDGTDVDSNNSVKNQIFKYNVPSGDTLSNSSKIQVEEDFVLLDSSYFSEMSTTMEYDVSQKDTVKGESKETAQSQELSSTKKTYSGEIILESLPDITSAFNLNSSSVLRNNSLESNESSNETLFMSSNATFSDSTNATSSDSSTATFADFSNTTFSNATFSDFSNRISQMSDSSNATLSDSSNATLSDSSNATLSDSSNATLSDSSNATLSNSSNATFSDSSNKTFSDSSNATLFGVSYSSNTTLSDFSLESEMTEYILSSANDTIKSHSEVVSNTSQLSSSESTENISLLYGSLNASSMKNDSESESEEEVVIYLNKNHSEAILTSHLDQKEEHWGYESKHELVHKELPDHMNKYVKDKSAANSNKPKIEKEKKKVYQRVKPKKGYGMKTKKSKDYKPQPRSSFSPRGFGPSVLTPRGSRPVSSEDELTEKPIVIGVPRRDFNDYELYIPKQDQEADFDGLLDHPEEYEYVEYKDPYSKTADVQALDATSQHLLKMAGDKNTRTYFISVEEEEWDYAGYGQRRLDKTAQNERPTVFRKVVFRRYLDSTFSIRDIRGEMDEHLGILGPLIKAEVDQTVMVFFRNRASRPYSLHANGVKYLKQMEGLSYDDESPYWYKQDDAVPPNGTFTYMWTINPKSGPQNNESDCRTWTYYSAVNPERDINSGLIGPLLVCRKGTLDKKPEDRREFVLLFMTFDENKSWLYEENRQRIERKNRRVVMDPNFQDNLKFDAINGIIYSLKGLRMYTNQLAKWHLINMGSPKDLHSVHFHGQTFINKELKDHRQGVYPLLPGGFATLEMLPSKPGLWQLESEVGLSQQRGMQTLFLVLDNVCDHPLGLISGTVQDEQITASDTRGQWYPHLARLHNTGKYNAWSTSSEPGQYLQVDFQRPVVISKVATQGAKQFLTHNFVLNYTISYSTDKKKWIYYKGDSDAVRKTFEGNAEAYETKENIFFPPLIGRYVRLHPLHSYNFPTVRLEYYGCELDGCSVPLGMEKGLIDDSKITASSVASNWYSGQWHPWYARLNKQGTANAWQAKNNDIQPWIQVELKEVKKITGIVTQGAKSMGNEMFVRSYILEYSEDGRRWMKYTDDDDYEQKLFQGNTDNNGQIKNYIYPPIFSRFIRIIPKQWQKSVTMRIELLGCDFE

Target ORF information:

RefSeq Version	NM_001328531.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio coagulation factor V (f5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328531.1

ORF Insert Sequence:

ATGAAACTCC GCTCCTGGCC AGGGGCCCTT CATGGTTTGG CCCTCCTGGC ATTCCTGTGT 
CATTGTGCAA CAGCTGTGGA GAGACATTAC TATATTGCAG CTGTTAATAT TAACTGGGAC 
TACACATCTG GACAGCAAAG AACCGGACAA TCATATAAAA AAGTAGTATA CAGGGAATAT 
AATGAGGGAT TCAAACAGCC AAAAGCTCAT CCCTTGTCCT CAGGGTTACT TGGGCCTACA 
TTGCGTGGAC AAGAGGGTGA CACAATCATC GTCACGTTCA GGAACATGGC AGATCATCCT 
TGCAGCCTCC ACCCCCATGG CATTGCTTAC GGGAAGCAGT CAGAGGGGTC TTTATACTTT 
GACAACACGT CACTCCTTGA GAAAAATGAT GATGTAATTC AACCTGGTGA AGAGCACACT 
TACCAGTGGG ACGTGACGTC TGATGTGACC CCTACAGCTG CAGACCCACC ATGCATCACC 
TACACATACT TGTCTCACTT TGACATTGTC CGAGACTACA ATACAGGGTT AATAGGCCCA 
ATGCTGATCT GCAAGAAAGG CACCCTAGAC GACTCAGGAA ATCAGATTCA TTTCCATCAA 
GAGTCTGTGC TGCTGTTTGG AGTATTTGAT GAGAACAAGA GCTGGTACAG CACTGGGGAC 
TCTCCACAGC CTCTGAATGT CAAATACACA ATAAACGGCT ACACAAATGG CTCAGTACCA 
GATCTGGACA TCTGTGCCCA CTCTAAAGTC AGCTGGCACC TGCTGGGAAT GAGCTCAGAG 
CCAGAGCTTT TCTCTGTACA CTTCAATGGG CAAGTCCTTC TGCACGATGG GCACAAAACT 
TCAGCTGTTG GCATTATTAG TGGAACTGCC ACCAGTGCCA GTATGACCGG TGTCCACCCT 
GGCCGCTGGC TCGTTTCCTC GCACATTAGC AAGCACTTGG AAGCTGGTTT GCACGGGTAC 
CTAAATATCA GGAAGTGTGA TGAGTATACG GCGCCAAAAA GACGGCTTAC CATTGAACAG 
AAAAAAGAGA GTCAAGAGTG GACTTACTAT ATGGCAGCAG AAGAGGTCAT CTGGGATTAT 
GCGCCAAATA TGCCAGAAAA CATGGACGGG GATTTCCGGT CAAAATATTT AAAGCAGGGG 
CCTCAGCGGA TAGGTAAAAA ATATAAAAAA GCTGTGTTTA CTCAGTATAA AGATGGCATG 
TTTAAAGAGA GAGCGGAAGA TAAGCAGAGG AAGAGAGAGC TTGGAATTCT TGGCCCAGTG 
ATTAGAGCTC AAATCAGAGA CATCATTAAG ATTGTCTTTA AAAACAAAGC ATCACGTCCC 
TATAGTATCT ATCCCCATGG ACTGACCATA GATAAAGCAG CAGAAGGAGC CAGTTACCCT 
CAGGGAGGGA ACCAAACATA CAGCGTTCAG CCAGGAGAGA CGTACACATA TACATGGAGT 
GTGACTGAGG AAGACGTACC AACGGACAGT GACCCCAGAT GTCTAACCAG GATGTACCAC 
AGTGCAGTGG ACGCTCCTCG AGACATTGCT TCAGGTCTTG TTGGCCCTCT GCTCATCTGT 
AAGAGCCAGT CTCTTAACAA AAAGAATGTC CAGCTAAAAG CTGACAAGGA GCAGCATGCC 
ATGTTTACCG TTTTTGATGA GAACAAGAGC TGGTACCAAG ATGAGAACAT TAACACATAC 
TGCAGTGATC CCAAAAAAGT GAAGAAAGAC GATCCCGAGT TTTACAAGTC AAATGTCATG 
CACACAATCA ATGGTTATGT ATATGAAAGC GGCCAAGAAT TGGGATTTTG TCATGGTGAA 
ATTGTGACTT GGCATGTGTC GAGTGTTGGG GAACAGGATT ACATCCAGAC TGCTACTTTC 
TATGGTCATA CATTTGAGCT AAAAAACAGA GAAGAGGATA TACTCAGTCT ATTTCCTATG 
ACTGGTGAAA CTATCACGAT GAACATGGTT AATATAGGTA TTTGGCTTTT GGCATCATTG 
AACTCGCATG ATTCCACTAA AGGGATGAGG GTGAAATTCA AAGACCTCGA ATGCTTCAGA 
GATTATGTAA TAGAATATGA TTATGAGGAC GGGAAATTCA CTGCATGGAA ACCACCGACT 
ATTAATGAAA TCAAAAAAGA GGAACCGGTC CGTGCAAGAC CTGACGTGGT CGACGAGTAC 
TCTGATTTAT TTGCTGAAAC CCTCAACTTA AGGACGTTCA ACAATGTTAA AGATGAAGTT 
GAGATAATCG ACTTGACATT TCTAGATCAA GATGATGGTT TGTTGCCCAT TGTTGAAGAA 
AAAAGCCTGG GATCAAGCAA CGAGAACTTA CACAATGCTA CTTTACAATC TTTCATTGAG 
ACTCATGGTT TATTAATGGA GGAAGGTGAT TTAGACAAGG GAGAATCTTC TAATAAAGTC 
TTGAATGACA GCACAGACAA GGCATTGCTA GAGACAACAA CTACTTTTGA TTCCAATAGA 
GTTGTCGCAT TAAACAACGA AACGGACAGT ATAATTTTAG ATTTTCCAAT TGTAGAAAGA 
AAGGTTCGTA GTGCACCATC AAAGCCAATG AACGAACCTG AAAGTGTCAC TATGAACTTT 
AAAACAACAG AACATATCAA TTCATCATTA GAGAGAATCA ATGCTATATA TTCCCCAATA 
ACTGAAACAA ACATCAACAC AATGACTGAG ACACACACTG ATTTCAGTAT CACTCCATTT 
GATGGTTCAA CTGGAGAAAT GAACTTCACA CTAGAGGATG ACACCGCACT TCTAAATTCA 
TCTGAATCAG AGCCCCTGCA ATCAAACCAA AACTCCGAAA ACAGAATAGC TTTTCAAGAA 
GAGCTAAATG CAAAAGATGG CACAGATGTT GACAGTAATA ATTCTGTGAA AAATCAGATC 
TTCAAATACA ACGTGCCTAG TGGTGACACT CTGTCCAACA GCTCAAAGAT ACAAGTTGAG 
GAGGATTTTG TGCTTCTGGA CAGTAGTTAT TTTTCAGAAA TGTCAACAAC TATGGAATAT 
GATGTTTCAC AAAAGGACAC TGTAAAAGGT GAATCAAAAG AGACTGCACA AAGTCAAGAA 
CTCAGCAGCA CAAAGAAAAC TTACTCTGGT GAAATAATTT TGGAGAGTCT TCCTGACATA 
ACAAGTGCTT TTAATCTGAA TTCATCTTCA GTTTTGAGAA ACAACAGTTT GGAGAGCAAT 
GAGTCCTCAA ATGAAACTTT GTTCATGTCT TCAAATGCTA CCTTCTCTGA TTCAACAAAT 
GCAACCTCGT CTGATTCCTC AACAGCAACC TTCGCTGATT TCTCAAACAC AACCTTCTCA 
AACGCAACCT TCTCTGATTT CTCAAATAGG ATTTCTCAAA TGTCTGATTC CTCAAATGCA 
ACCTTGTCTG ATTCCTCAAA TGCAACCTTG TCTGATTCCT CAAATGCAAC ATTGTCTGAT 
TCCTCAAATG CAACATTGTC TGATTCCTCA AATGCAACAT TGTCTAATTC CTCAAATGCA 
ACCTTCTCTG ATTCCTCAAA CAAAACCTTC TCTGATTCCT CAAATGCAAC CTTGTTTGGT 
GTTTCATATT CCTCAAACAC AACCTTGTCT GACTTCAGTC TTGAATCTGA AATGACAGAG 
TATATACTGT CATCTGCTAA TGATACAATA AAATCTCACA GTGAAGTTGT AAGCAACACT 
TCACAGCTTT CCTCTTCAGA AAGCACAGAG AATATATCGT TGTTGTATGG CTCTCTCAAT 
GCTTCATCCA TGAAGAACGA CTCAGAAAGT GAAAGCGAAG AAGAAGTGGT TATCTACCTA 
AATAAAAATC ACAGTGAGGC AATCCTCACA TCTCATCTCG ACCAAAAAGA AGAGCACTGG 
GGTTATGAAA GCAAACACGA GCTGGTTCAT AAGGAACTCC CAGATCACAT GAACAAATAT 
GTCAAAGATA AGTCTGCAGC AAACTCGAAC AAGCCAAAGA TCGAAAAGGA AAAGAAAAAA 
GTTTACCAAA GAGTAAAGCC AAAAAAGGGG TACGGGATGA AGACGAAGAA AAGTAAAGAC 
TACAAACCGC AGCCGCGTTC GTCATTTTCT CCAAGAGGTT TTGGACCATC TGTGTTAACT 
CCAAGGGGAA GCAGACCTGT CTCTAGCGAG GATGAACTGA CGGAGAAACC TATTGTCATA 
GGAGTGCCAA GACGGGATTT CAATGATTAT GAGTTATATA TTCCAAAACA AGACCAGGAA 
GCAGATTTTG ATGGCTTGCT TGATCACCCA GAAGAATATG AATATGTAGA GTATAAAGAC 
CCTTACAGCA AAACAGCAGA TGTTCAAGCT CTTGACGCAA CATCCCAGCA TCTCTTAAAA 
ATGGCTGGAG ACAAGAATAC CAGGACCTAT TTCATCTCGG TTGAAGAAGA AGAATGGGAT 
TATGCTGGCT ATGGGCAGAG GCGACTTGAT AAAACAGCTC AAAATGAGAG GCCCACGGTT 
TTCAGAAAAG TGGTGTTCCG AAGGTACCTG GACAGCACCT TTAGCATCCG AGACATCCGA 
GGAGAAATGG ACGAACACCT GGGAATTCTT GGTCCGCTCA TTAAAGCGGA AGTTGATCAG 
ACCGTTATGG TATTTTTCAG GAACCGTGCA AGTCGTCCAT ACTCTTTGCA TGCAAATGGA 
GTAAAATACT TAAAACAAAT GGAAGGCCTG AGTTATGATG ATGAATCTCC ATACTGGTAC 
AAACAGGACG ATGCTGTTCC ACCCAATGGC ACCTTTACCT ACATGTGGAC CATAAATCCC 
AAATCTGGAC CTCAAAATAA TGAATCTGAC TGCCGAACTT GGACCTACTA CTCTGCAGTG 
AATCCTGAGA GAGATATAAA CTCAGGGTTG ATTGGGCCAC TTCTGGTGTG CAGGAAGGGT 
ACACTTGATA AAAAGCCTGA AGACAGAAGA GAGTTTGTCC TGCTTTTCAT GACATTTGAT 
GAAAATAAAA GCTGGCTCTA TGAAGAGAAT CGGCAGAGGA TTGAAAGGAA AAACAGAAGA 
GTGGTCATGG ATCCAAATTT CCAAGATAAT TTAAAGTTTG ATGCTATCAA TGGTATCATC 
TACAGTCTCA AAGGACTTCG AATGTACACA AATCAGCTGG CGAAGTGGCA TCTGATCAAC 
ATGGGCTCTC CTAAAGACCT CCACAGTGTT CACTTCCATG GACAAACATT CATAAATAAA 
GAATTAAAGG ACCACCGTCA GGGCGTTTAC CCGCTTTTAC CAGGTGGATT TGCCACGCTG 
GAAATGCTGC CTTCAAAGCC TGGTCTCTGG CAGCTGGAGT CTGAAGTCGG ACTCTCACAG 
CAGAGGGGAA TGCAAACGCT ATTTCTTGTG TTGGATAATG TCTGTGATCA CCCTCTTGGT 
CTCATCTCTG GGACTGTGCA AGATGAGCAA ATAACAGCAT CTGACACTAG AGGACAATGG 
TATCCTCATT TGGCCAGACT TCATAATACA GGGAAATACA ATGCATGGAG TACATCGTCA 
GAGCCAGGAC AGTACTTACA GGTGGACTTC CAGAGGCCGG TGGTGATTAG TAAAGTCGCC 
ACACAAGGAG CCAAACAATT TTTGACACAC AACTTTGTGT TGAACTACAC CATTTCCTAC 
AGCACTGACA AAAAGAAGTG GATCTACTAC AAGGGAGACA GTGATGCTGT CAGAAAGACT 
TTTGAAGGAA ATGCTGAAGC TTATGAGACG AAGGAAAATA TATTTTTCCC ACCTCTGATT 
GGTCGATATG TGAGACTGCA TCCTTTACAT TCTTACAACT TCCCGACTGT CCGTTTGGAG 
TATTATGGTT GTGAGCTTGA TGGCTGCTCT GTGCCATTAG GAATGGAAAA AGGTCTTATT 
GATGACAGTA AAATCACTGC CAGTTCTGTG GCATCCAACT GGTACTCCGG ACAGTGGCAT 
CCTTGGTATG CTCGCCTAAA TAAACAAGGA ACCGCCAATG CATGGCAAGC AAAGAACAAT 
GACATTCAGC CTTGGATCCA GGTGGAACTC AAAGAAGTAA AGAAGATAAC TGGAATTGTA 
ACACAGGGAG CCAAATCAAT GGGCAATGAA ATGTTTGTGA GATCTTATAT TTTGGAATAT 
AGTGAAGATG GCAGGCGATG GATGAAGTAC ACAGATGATG ATGATTATGA GCAAAAGCTA 
TTTCAAGGCA ACACAGATAA CAATGGCCAA ATAAAGAACT ACATCTACCC GCCGATATTT 
TCCAGATTCA TCAGAATAAT ACCAAAACAA TGGCAGAAAT CCGTCACCAT GAGAATTGAG 
CTGCTGGGTT GTGATTTCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of factor V in zebrafish demonstrates minimal levels needed for early hemostasis.
Blood advances3(11)1670-1680(2019 Jun)
Weyand AC,Grzegorski SJ,Rost MS,Lavik KI,Ferguson AC,Menegatti M,Richter CE,Asselta R,Duga S,Peyvandi F,Shavit JA

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Systems pharmacology of adiposity reveals inhibition of EP300 as a common therapeutic mechanism of caloric restriction and resveratrol for obesity.
Frontiers in pharmacology6199(2015)
Nishimura Y,Sasagawa S,Ariyoshi M,Ichikawa S,Shimada Y,Kawaguchi K,Kawase R,Yamamoto R,Uehara T,Yanai T,Takata R,Tanaka T

The transcriptomics of glucocorticoid receptor signaling in developing zebrafish.
PloS one8(11)e80726(2013)
Nesan D,Vijayan MM

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f5 cDNA ORF clone, Danio rerio(zebrafish)

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