Adamts5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adamts5
Entrez Gene ID	304135
Full Name	ADAM metallopeptidase with thrombospondin type 1 motif, 5
General protein information	Preferred Names ADAM metallopeptidase with thrombospondin type 1 motif, 5 Names A disintegrin and metalloproteinase with thrombospondin motifs 5 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q11
Summary	human homolog catalyzes the degradation of aggrecan, a component of cartilage extracellular matrix [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_198761.1	NP_942056.1	A disintegrin and metalloproteinase with thrombospondin motifs 5 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Adamts5	NP_035912.2
Canis lupus familiaris (dog)	ADAMTS5	XP_851118.2
Bos taurus (cattle)	ADAMTS5	NP_001159987.1
Gallus gallus (chicken)	ADAMTS5	XP_425541.4
Xenopus tropicalis (tropical clawed frog)	adamts5	XP_002935740.1
Pan troglodytes (chimpanzee)	ADAMTS5	XP_531407.2
Danio rerio (zebrafish)	adamts5	XP_693454.6
Homo sapiens (human)	ADAMTS5	NP_008969.2
Macaca mulatta (Rhesus monkey)	ADAMTS5	XP_001103439.1
Rattus norvegicus (Norway rat)	Adamts5	NP_942056.1

Gene Ontology
Bibliography

Related articles in PubMed

MiR-132-3p regulates ADAMTS-5 expression and promotes chondrogenic differentiation of rat mesenchymal stem cells.
Zhou X, Luo D, Sun H, Qi Y, Xu W, Jin X, Li C, Lin Z, Li G
Journal of cellular biochemistry119(3)2579-2587(2018 03)

Loss of ADAMTS5 enhances brown adipose tissue mass and promotes browning of white adipose tissue via CREB signaling.
Bauters D, Cobbaut M, Geys L, Van Lint J, Hemmeryckx B, Lijnen HR
Molecular metabolism6(7)715-724(2017 07)

Reducing dietary loading decreases mouse temporomandibular joint degradation induced by anterior crossbite prosthesis.
Liu YD, Liao LF, Zhang HY, Lu L, Jiao K, Zhang M, Zhang J, He JJ, Wu YP, Chen D, Wang MQ
Osteoarthritis and cartilage22(2)302-12(2014 Feb)

Osteoprotegerin promotes the proliferation of chondrocytes and affects the expression of ADAMTS-5 and TIMP-4 through MEK/ERK signaling.
Feng ZY, He ZN, Zhang B, Chen Z
Molecular medicine reports8(6)1669-79(2013 Dec)

Biosynthesis and expression of a disintegrin-like and metalloproteinase domain with thrombospondin-1 repeats-15: a novel versican-cleaving proteoglycanase.
Dancevic CM, Fraser FW, Smith AD, Stupka N, Ward AC, McCulloch DR
The Journal of biological chemistry288(52)37267-76(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Adamts5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adamts5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04989	NM_198761.1 Latest version!	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1 motif, 5 (Adamts5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adamts5 ( NM_198761.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_942056.1 Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1 motif, 5 (Adamts5), mRNA.

Price & Availability↑

CloneID	ORa04989
Clone ID Related Accession (Same CDS sequence)	NM_198761.1
Accession Version	NM_198761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1539273600000
Organism	Rattus norvegicus(Norway rat)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 5 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY382879.1. On Dec 17, 2003 this sequence version replaced XM_221699.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY382879.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689602, SAMN01906355 [ECO:0000348] ##Evidence-Data-END##

ATGCTACTCG AGTGGGCGTC CTTGCTGCTG CTACTGCTGC TGCTGTGCGC GTCCTGCCTG 
GCCCTGGCGG CTGACAACCC TGCCGCGGCA CCTGCCCAGG ATAAAACCAG GCAGCCTCGG 
GCTGCTGCAG CGGCCGCCCA GCCCGACCAG CGGCAGTGGG AGGAAACACA GGAGCGGGGC 
CATCCGCAAC CCTTGGCCAG GCAGCGCAGG AGCAGCGGGC TGGTGCAGAA TATAGACCAA 
CTCTACTCTG GCGGTGGCAA AGTGGGCTAC CTTGTCTACG CGGGCGGCCG GAGGTTCCTG 
CTGGACCTGG AGAGGGATGA CACAGTGGGT GCTGCTGGTG GCATCGTTAC TGCAGGAGGA 
GGGCTGAGCG CATCCTCTGG CCACAGGGGT CACTGCTTCT ACAGAGGCAC TGTGGACGGC 
AGCCCTCGAT CCCTAGCTGT CTTTGACCTC TGTGGGGGTC TCGATGGCTT CTTCGCAGTC 
AAGCATGCGC GCTACACTCT GAAGCCGCTC TTGCGTGGGT CCTGGGCAGA GTCCGAACGA 
GTTTACGGGG ATGGGTCTTC ACGCATCCTG CATGTCTACA CCCGCGAGGG CTTCAGCTTC 
GAGGCCCTGC CGCCACGCAC CAGTTGCGAG ACTCCAGCGT CCCCGTCTGG GCCCCAAGAG 
AGCCCCTCGG TGCACAGTAG TTCTAGGCGA CGCACAGAAC TGGCACCGCA GCTGCTGGAC 
CATTCAGCTT TCTCGCCAGC TGGGAACGCG GGACCTCAGA CGTGGTGGAG GCGGAGGCGC 
CGTTCCATCT CCAGGGCCCG CCAGGTGGAG CTCCTCTTGG TGGCTGACTC TTCCATGGCC 
AAGATGTATG GGCGGGGCCT GCAGCATTAC CTGCTGACCC TGGCCTCTAT TGCCAACCGG 
CTGTACAGTC ATGCAAGCAT CGAGAACCAC ATCCGCCTGG CCGTAGTGAA AGTGGTGGTG 
CTGACCGACA AGAGTCTGGA GGTGAGCAAG AACGCGGCCA CGACCCTCAA GAACTTTTGC 
AAATGGCAGC ACCAACACAA CCAGCTAGGT GATGACCATG AGGAGCACTA CGATGCAGCC 
ATCCTGTTCA CCAGAGAGGA TTTATGTGGG CATCATTCAT GTGACACCCT GGGAATGGCA 
GACGTTGGGA CCATATGTTC TCCGGAGCGC AGCTGCGCTG TGATTGAAGA TGATGGCCTC 
CATGCAGCTT TCACTGTGGC TCACGAAATT GGACATCTAC TTGGCCTCTC TCACGACGAT 
TCCAAATTCT GTGAAGAGAA TTTTGGTTCT ACAGAAGACA AGCGTTTAAT GTCTTCAATC 
CTTACCAGCA TTGATGCATC CAAGCCCTGG TCCAAATGCA CTTCAGCCAC GATCACAGAA 
TTTCTGGATG ACGGTCATGG TAACTGTTTA CTAGATGTAC CACGGAAGCA GATTCTGGGC 
CCCGAGGAAC TCCCAGGACA GACCTATGAT GCCACCCAGC AGTGCAACTT GACATTTGGG 
CCTGAATATT CTGTGTGCCC TGGCATGGAT GTCTGTGCAC GGCTGTGGTG TGCTGTGGTG 
CGCCAAGGCC AAATGGTGTG TCTGACCAAG AAGTTGCCTG CCGTGGAGGG CACTCCCTGT 
GGTAAAGGAA GAATCTGCCT GCAAGGGAAA TGTGTGGACA AAACTAAGAA AAAATATTAC 
TCGACATCAA GCCATGGAAA TTGGGGGTCC TGGGGCCCCT GGGGTCAGTG TTCTCGCTCT 
TGCGGGGGAG GAGTACAGTT TGCCTACCGC CATTGCAATA ACCCCGCACC TCGAAACAGT 
GGCCGCTACT GCACAGGGAA GAGGGCCATA TACCGTTCCT GCAGTGTCAT ACCCTGCCCA 
CCTAACGGCA AATCTTTCCG CCACGAGCAG TGTGAAGCCA AAAATGGCTA TCAGTCCGAT 
GCAAAAGGAG TCAAAACATT TGTAGAATGG GTTCCCAAAT ACGCAGGTGT CCTGCCGGCA 
GACGTGTGCA AGCTTACGTG CAGAGCTAAG GGCACTGGCT ATTACGTGGT CTTTTCTCCA 
AAGGTTACAG ATGGGACAGA ATGTAGACCC TACAGCAACT CCGTGTGTGT CCGAGGAAGG 
TGCGTGAGAA CGGGGTGTGA CGGCATCATC GGCTCAAAGC TACAGTATGA CAAGTGTGGA 
GTGTGTGGAG GGGATAACTC CAGTTGTACA AAGATTATCG GAACCTTCAA TAAAAAAAGC 
AAGGGTTATA CTGACGTTGT GAGGATCCCT GAAGGAGCAA CCCACATAAA AGTCCGACAG 
TTCAAAGCCA AAGACCAGAC TAGATTCACT GCTTACTTAG CCCTAAAGAA GAAAACTGGC 
GAGTACCTTA TCAACGGCAA GTACATGATC TCCACTTCAG AGACCATCAT CGACATCAAT 
GGTACCGTCA TGAACTACAG TGGGTGGAGT CACAGAGATG ATTTTTTACA TGGGATGGGC 
TATTCAGCCA CAAAGGAAAT TCTGATTGTG CAGATCCTTG CAACAGACCC AACTAAAGCA 
TTAGACGTCC GTTACAGCTT TTTTGTTCCC AAGAAGACCA CTCAAAAAGT GAATTCCGTC 
ATTAGCCACA GCAGCAACAA AGTGGGACTA CACTCCCCGC AGCTACAGTG GGTGACAGGC 
CCCTGGCTGG CCTGCTCTAG GACCTGTGAC ACAGGCTGGC ACACCAGGAC CGTGCAGTGC 
CAGGATGGAA ACAGGAAATT AGCTAAGGGA TGCATTCTCT CTCAGAGGCC TTCTGCGTTT 
AAGCAATGTT TGCTCAAGAA ATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942056.1
CDS	1..2787
Translation	MLLEWASLLLLLLLLCASCLALAADNPAAAPAQDKTRQPRAAAAAAQPDQRQWEETQERGHPQPLARQRRSSGLVQNIDQLYSGGGKVGYLVYAGGRRFLLDLERDDTVGAAGGIVTAGGGLSASSGHRGHCFYRGTVDGSPRSLAVFDLCGGLDGFFAVKHARYTLKPLLRGSWAESERVYGDGSSRILHVYTREGFSFEALPPRTSCETPASPSGPQESPSVHSSSRRRTELAPQLLDHSAFSPAGNAGPQTWWRRRRRSISRARQVELLLVADSSMAKMYGRGLQHYLLTLASIANRLYSHASIENHIRLAVVKVVVLTDKSLEVSKNAATTLKNFCKWQHQHNQLGDDHEEHYDAAILFTREDLCGHHSCDTLGMADVGTICSPERSCAVIEDDGLHAAFTVAHEIGHLLGLSHDDSKFCEENFGSTEDKRLMSSILTSIDASKPWSKCTSATITEFLDDGHGNCLLDVPRKQILGPEELPGQTYDATQQCNLTFGPEYSVCPGMDVCARLWCAVVRQGQMVCLTKKLPAVEGTPCGKGRICLQGKCVDKTKKKYYSTSSHGNWGSWGPWGQCSRSCGGGVQFAYRHCNNPAPRNSGRYCTGKRAIYRSCSVIPCPPNGKSFRHEQCEAKNGYQSDAKGVKTFVEWVPKYAGVLPADVCKLTCRAKGTGYYVVFSPKVTDGTECRPYSNSVCVRGRCVRTGCDGIIGSKLQYDKCGVCGGDNSSCTKIIGTFNKKSKGYTDVVRIPEGATHIKVRQFKAKDQTRFTAYLALKKKTGEYLINGKYMISTSETIIDINGTVMNYSGWSHRDDFLHGMGYSATKEILIVQILATDPTKALDVRYSFFVPKKTTQKVNSVISHSSNKVGLHSPQLQWVTGPWLACSRTCDTGWHTRTVQCQDGNRKLAKGCILSQRPSAFKQCLLKKC

Target ORF information:

RefSeq Version	NM_198761.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1 motif, 5 (Adamts5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198761.1

ORF Insert Sequence:

ATGCTACTCG AGTGGGCGTC CTTGCTGCTG CTACTGCTGC TGCTGTGCGC GTCCTGCCTG 
GCCCTGGCGG CTGACAACCC TGCCGCGGCA CCTGCCCAGG ATAAAACCAG GCAGCCTCGG 
GCTGCTGCAG CGGCCGCCCA GCCCGACCAG CGGCAGTGGG AGGAAACACA GGAGCGGGGC 
CATCCGCAAC CCTTGGCCAG GCAGCGCAGG AGCAGCGGGC TGGTGCAGAA TATAGACCAA 
CTCTACTCTG GCGGTGGCAA AGTGGGCTAC CTTGTCTACG CGGGCGGCCG GAGGTTCCTG 
CTGGACCTGG AGAGGGATGA CACAGTGGGT GCTGCTGGTG GCATCGTTAC TGCAGGAGGA 
GGGCTGAGCG CATCCTCTGG CCACAGGGGT CACTGCTTCT ACAGAGGCAC TGTGGACGGC 
AGCCCTCGAT CCCTAGCTGT CTTTGACCTC TGTGGGGGTC TCGATGGCTT CTTCGCAGTC 
AAGCATGCGC GCTACACTCT GAAGCCGCTC TTGCGTGGGT CCTGGGCAGA GTCCGAACGA 
GTTTACGGGG ATGGGTCTTC ACGCATCCTG CATGTCTACA CCCGCGAGGG CTTCAGCTTC 
GAGGCCCTGC CGCCACGCAC CAGTTGCGAG ACTCCAGCGT CCCCGTCTGG GCCCCAAGAG 
AGCCCCTCGG TGCACAGTAG TTCTAGGCGA CGCACAGAAC TGGCACCGCA GCTGCTGGAC 
CATTCAGCTT TCTCGCCAGC TGGGAACGCG GGACCTCAGA CGTGGTGGAG GCGGAGGCGC 
CGTTCCATCT CCAGGGCCCG CCAGGTGGAG CTCCTCTTGG TGGCTGACTC TTCCATGGCC 
AAGATGTATG GGCGGGGCCT GCAGCATTAC CTGCTGACCC TGGCCTCTAT TGCCAACCGG 
CTGTACAGTC ATGCAAGCAT CGAGAACCAC ATCCGCCTGG CCGTAGTGAA AGTGGTGGTG 
CTGACCGACA AGAGTCTGGA GGTGAGCAAG AACGCGGCCA CGACCCTCAA GAACTTTTGC 
AAATGGCAGC ACCAACACAA CCAGCTAGGT GATGACCATG AGGAGCACTA CGATGCAGCC 
ATCCTGTTCA CCAGAGAGGA TTTATGTGGG CATCATTCAT GTGACACCCT GGGAATGGCA 
GACGTTGGGA CCATATGTTC TCCGGAGCGC AGCTGCGCTG TGATTGAAGA TGATGGCCTC 
CATGCAGCTT TCACTGTGGC TCACGAAATT GGACATCTAC TTGGCCTCTC TCACGACGAT 
TCCAAATTCT GTGAAGAGAA TTTTGGTTCT ACAGAAGACA AGCGTTTAAT GTCTTCAATC 
CTTACCAGCA TTGATGCATC CAAGCCCTGG TCCAAATGCA CTTCAGCCAC GATCACAGAA 
TTTCTGGATG ACGGTCATGG TAACTGTTTA CTAGATGTAC CACGGAAGCA GATTCTGGGC 
CCCGAGGAAC TCCCAGGACA GACCTATGAT GCCACCCAGC AGTGCAACTT GACATTTGGG 
CCTGAATATT CTGTGTGCCC TGGCATGGAT GTCTGTGCAC GGCTGTGGTG TGCTGTGGTG 
CGCCAAGGCC AAATGGTGTG TCTGACCAAG AAGTTGCCTG CCGTGGAGGG CACTCCCTGT 
GGTAAAGGAA GAATCTGCCT GCAAGGGAAA TGTGTGGACA AAACTAAGAA AAAATATTAC 
TCGACATCAA GCCATGGAAA TTGGGGGTCC TGGGGCCCCT GGGGTCAGTG TTCTCGCTCT 
TGCGGGGGAG GAGTACAGTT TGCCTACCGC CATTGCAATA ACCCCGCACC TCGAAACAGT 
GGCCGCTACT GCACAGGGAA GAGGGCCATA TACCGTTCCT GCAGTGTCAT ACCCTGCCCA 
CCTAACGGCA AATCTTTCCG CCACGAGCAG TGTGAAGCCA AAAATGGCTA TCAGTCCGAT 
GCAAAAGGAG TCAAAACATT TGTAGAATGG GTTCCCAAAT ACGCAGGTGT CCTGCCGGCA 
GACGTGTGCA AGCTTACGTG CAGAGCTAAG GGCACTGGCT ATTACGTGGT CTTTTCTCCA 
AAGGTTACAG ATGGGACAGA ATGTAGACCC TACAGCAACT CCGTGTGTGT CCGAGGAAGG 
TGCGTGAGAA CGGGGTGTGA CGGCATCATC GGCTCAAAGC TACAGTATGA CAAGTGTGGA 
GTGTGTGGAG GGGATAACTC CAGTTGTACA AAGATTATCG GAACCTTCAA TAAAAAAAGC 
AAGGGTTATA CTGACGTTGT GAGGATCCCT GAAGGAGCAA CCCACATAAA AGTCCGACAG 
TTCAAAGCCA AAGACCAGAC TAGATTCACT GCTTACTTAG CCCTAAAGAA GAAAACTGGC 
GAGTACCTTA TCAACGGCAA GTACATGATC TCCACTTCAG AGACCATCAT CGACATCAAT 
GGTACCGTCA TGAACTACAG TGGGTGGAGT CACAGAGATG ATTTTTTACA TGGGATGGGC 
TATTCAGCCA CAAAGGAAAT TCTGATTGTG CAGATCCTTG CAACAGACCC AACTAAAGCA 
TTAGACGTCC GTTACAGCTT TTTTGTTCCC AAGAAGACCA CTCAAAAAGT GAATTCCGTC 
ATTAGCCACA GCAGCAACAA AGTGGGACTA CACTCCCCGC AGCTACAGTG GGTGACAGGC 
CCCTGGCTGG CCTGCTCTAG GACCTGTGAC ACAGGCTGGC ACACCAGGAC CGTGCAGTGC 
CAGGATGGAA ACAGGAAATT AGCTAAGGGA TGCATTCTCT CTCAGAGGCC TTCTGCGTTT 
AAGCAATGTT TGCTCAAGAA ATGTTAG