Ube4b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube4b
Entrez Gene ID	298652
Full Name	ubiquitination factor E4B
Synonyms	ufd2a
General protein information	Preferred Names ubiquitination factor E4B Names ubiquitin conjugation factor E4 B ubiquitination factor E4B (UFD2 homolog, yeast) ubiquitination factor E4B, UFD2 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239387.3	XP_006239449.1	ubiquitin conjugation factor E4 B isoform X1
NM_001271198.1	NP_001258127.1	ubiquitin conjugation factor E4 B
XM_017593310.1	XP_017448799.1	ubiquitin conjugation factor E4 B isoform X2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F05269g	XP_455319.1
Eremothecium gossypii	AGOS_ADR096C	NP_984192.1
Neurospora crassa	NCU03357	XP_956466.1
Pan troglodytes (chimpanzee)	UBE4B	XP_001161561.1
Schizosaccharomyces pombe (fission yeast)	ufd2	NP_593630.1
Caenorhabditis elegans (roundworm)	ufd-2	NP_495691.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007870	XP_317622.4
Magnaporthe oryzae (rice blast fungus)	MGG_03255	XP_003716719.1
Rattus norvegicus (Norway rat)	Ube4b	NP_001258127.1
Drosophila melanogaster (fruit fly)	CG9934	NP_609597.1
Arabidopsis thaliana (thale cress)	AT5G15400	NP_568313.2
Mus musculus (house mouse)	Ube4b	NP_071305.2
Danio rerio (zebrafish)	ube4b	NP_919343.1
Xenopus tropicalis (tropical clawed frog)	ube4b	XP_004916084.1
Homo sapiens (human)	UBE4B	NP_001099032.1
Macaca mulatta (Rhesus monkey)	UBE4B	XP_001101836.1
Canis lupus familiaris (dog)	UBE4B	XP_005620583.1
Bos taurus (cattle)	UBE4B	NP_001095974.1
Gallus gallus (chicken)	UBE4B	XP_004947510.1
Saccharomyces cerevisiae (baker's yeast)	UFD2	NP_010091.2

Gene Ontology
Bibliography

Related articles in PubMed

Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis.
Starita LM, Pruneda JN, Lo RS, Fowler DM, Kim HJ, Hiatt JB, Shendure J, Brzovic PS, Fields S, Klevit RE
Proceedings of the National Academy of Sciences of the United States of America110(14)E1263-72(2013 Apr)

Structural and functional characterization of the monomeric U-box domain from E4B.
Nordquist KA, Dimitrova YN, Brzovic PS, Ridenour WB, Munro KA, Soss SE, Caprioli RM, Klevit RE, Chazin WJ
Biochemistry49(2)347-55(2010 Jan)

The WldS gene delays axonal but not somatic degeneration in a rat glaucoma model.
Beirowski B, Babetto E, Coleman MP, Martin KR
The European journal of neuroscience28(6)1166-79(2008 Sep)

Mammalian E4 is required for cardiac development and maintenance of the nervous system.
Kaneko-Oshikawa C, Nakagawa T, Yamada M, Yoshikawa H, Matsumoto M, Yada M, Hatakeyama S, Nakayama K, Nakayama KI
Molecular and cellular biology25(24)10953-64(2005 Dec)

Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis.
Okumura F, Hatakeyama S, Matsumoto M, Kamura T, Nakayama KI
The Journal of biological chemistry279(51)53533-43(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10519	NM_001271198.1 Latest version!	Rattus norvegicus ubiquitination factor E4B (Ube4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa28895	XM_006239387.3 Latest version!	Rattus norvegicus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa61189	XM_017593310.1 Latest version!	Rattus norvegicus ubiquitination factor E4B (Ube4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube4b ( NM_001271198.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258127.1 Rattus norvegicus ubiquitination factor E4B (Ube4b), mRNA.

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CloneID	ORa10519
Clone ID Related Accession (Same CDS sequence)	NM_001271198.1
Accession Version	NM_001271198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527609600000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin conjugation factor E4 B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07050466.1, AABR07050465.1, AABR07050464.1 and AABR07050463.1. On or before Sep 8, 2012 this sequence version replaced XM_001075491.3, XM_233679.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCAGGACCT 
CCAATAGCTG CATCAGTCCC AGGCCCCTCC CAGAGCCTTG GTCTCAATGT CCACAACACG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGTGAA 
GGCGTCAGTT CTCTTAGCAG CTCACCTTCC AACAGCCTTG AGACGCAATC TCAGTCCCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGT GTCTCCTGTG AGAAGAGCAT GTCCCAGGTG 
GATGTGGATT CTGGAATCGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAAGTGT CTGAAGAGCA GGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTGCTG ATGATGTCCA CTCAGACACG AGATGAAAAT 
CCGTTTGCCA GTCTGACGGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
AGAAACCTCA TGCTGAACAC AGGCTCCAGT TCGGGGACAA GTCCCATGTT CTGCAGCTTG 
GGTTCCTTCA GCGCCAGCTC ACTCTCCAGT TTGGGAGCCT CTGGTGGAGC AAGTACCTGG 
GATTCTTACA GTGACCATTT CACCATTGAA ACCTGCAAAG AGACGGACAT GCTGAACTAC 
CTCATCGAGT GCTTTGACCG AGTTGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC 
CAGCCAGCAG TCAGCCAGCT TCTGAGCAAC ATCCGCTCGC AGTGCATCTC CCACACCGCG 
CTAGTGCTAC AAGGGTCCCT CACACAGCCC AGGTCCTTGC AACAGCCATC CTTCCTCGTG 
CCGTACATGC TCTGCAGGAA TCTCCCGTAC GGCTTCATTC AAGAGCTGGT GAGGACCACT 
CACCAGGATG AAGAGGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCGCGT 
GCTGCCAAAG AGTGCTCCTT GGATAGCGAC TACTTTAAGT ACCCCCTCAT GGCACTGGGT 
GAACTCTGTG AAACCAAGTT CGGGAAGACA CACCCTATGT GCAATTTGGT CGCCTCCTTA 
CCCCTGTGGT TGCCGAAGTC CTTAAGCCCT GGTTCTGGGC GGGAGCTGCA GAGACTCTCC 
TACTTAGGGG CCTTCTTTAG CTTCTCTGTC TTCGCAGAGG ACGATGCAAA GGTGGTTGAG 
AAATACTTCT CAGGGCCTGC CATTACCCTG GAGAACACCC GTGTGGTCAG CCAGTCACTA 
CAGCATTACC TGGAGCTGGG ACGGCAAGAG CTTTTCAAGA TTCTGCATAG TATTTTGTTA 
AACGGCGAAA CTCGTGAAGC AGCACTCAGT TACATGGCAG CCATTGTCAA TGCCAATATG 
AAGAAAGCGC AGATGCAGGC AGATGATCGG TTGGTGTCTA CAGACGGGTT CATGCTGAAT 
CTCCTTTGGG TCCTGCAGCA GCTAAGTACA AAAATCAAGT TAGAAACGGT CGACCCCACT 
TACATATTCC ACCCGAGATG TCGGATCACT CTGCCAAACG ATGAGACACG GATAAATGCA 
ACGATGGAGG ATGTGAACGA GTGGCTGACA GAGCTCTATG GAGATCAGCC TCCATTTTCT 
GAGCCAAAAT TCCCTACAGA ATGCTTCTTT CTCACCCTGC ACGCTCACCA TCTCTCTATC 
CTGCCTAGTT GTCGTCGCTA CATTCGCCGG CTCCGAGCCA TCCGTGAGCT CAATAGAACC 
GTGGAAGATT TGAAAAATAA TGAAAGCCAG TGGAAAGATT CCCCACTGGC CACCAGACAC 
CGCGAAATGC TGAAGCGCTG TAAAACTCAG CTTAAGAAAC TGGTACGGTG CAAGGCCTGT 
GCTGACGCTG GCTTACTTGA CGAGAGCTTC CTAAGAAGAT GTCTGAATTT TTATGGCCTT 
CTCATTCAGC TGATGCTGCG CGTCCTGGAC CCTGCATATC CTGACATAAC ACTGCCTTTA 
AATTCAGAAG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TTTATGAGCC CTGCACTCAG 
GACATTGTGA TGTTCCTCGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAACCCTTAC 
CTAGTGGCCA AGCTCGTGGA GGTCATGTTT ATGACCAACC CCTCTGTTCA GCCGAGAACT 
CAGAAGTTTT TTGAGATGAT TGAGAACCAT CCTCTCTCTA CCAAATTGCT GGTACCGTCC 
CTCATGAAGT TCTATACAGA CGTCGAGCAT ACTGGAGCCA CCAGCGAGTT CTATGACAAG 
TTCACAATTC GCTATCACAT CAGCACTATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC 
CATGGCACCT TCATGGAGGA GTTCAATTCT GGAAAGCAGT TCGTTCGGTA TATCAACATG 
CTGATAAATG ACACGACCTT TTTACTGGAC GAAAGTCTGG AGTCTCTGAA GCGGATCCAT 
GAAGTGCAAG AGGAGATGAA GAACCAAGAG CAGTGGGACC AGCTGCCCCG GGATCAGCAG 
CAGGCCCGGC AGTCTCAGCT TGCTCAGGAC GAGCGTGTTT CCCGTTCCTA TCTCGCCCTG 
GCGACTGAAA CCGTGGACAT GTTCCATCTC CTCACCAAGC AAGTCCAGAA GCCGTTCCTG 
AGGCCAGAAC TTGGTCCCCG GTTAGCAGCC ATGCTGAACT TTAACCTGCA GCAGCTCTGC 
GGGCCCAAGT GCCGGGACCT CAAAGTGGAA AACCCAGAGA AGTACGGTTT TGAGCCAAAG 
AAGCTGTTGG ACCAACTGAC GGATATCTAC TTACAGCTGG ACTGTGCTCG CTTTGCTAAA 
GCCATCGCTG ATGACCAGCG ATCCTACAGC AAGGAACTGT TCGAAGAAGT CATTTCAAAG 
ATGCGGAAGG CAGGAATCAA ATCCACCATC GCAATAGAAA AGTTTAAGCT TCTTGCAGAG 
AAAGTGGAGG AAATCGTGGC AAAGAATGCT CGGGCGGAAA TAGACTACAG TGACGCCCCG 
GACGAGTTCA GAGACCCTCT GATGGACACC CTGATGACTG ATCCCGTGAG ACTGCCCTCT 
GGGACCATCA TGGACCGTTC TATCATCCTG CGGCATCTGC TCAACTCCCC CACCGACCCC 
TTCAACCGCC AGATGCTGAC TGAGAGCATG CTGGAGCCAG TGCCAGAGCT AAAGGAGCAG 
ATTCAGGCCT GGATGAGAGA GAAGCAGAGC AGTGACCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258127.1
CDS	841..4362
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASVPGPSQSLGLNVHNTTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCSLGSFSASSLSSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLARAAKECSLDSDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAIVNANMKKAQMQADDRLVSTDGFMLNLLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNEWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRVLDPAYPDITLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNQEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH

Target ORF information:

RefSeq Version	NM_001271198.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitination factor E4B (Ube4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271198.1

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCAGGACCT 
CCAATAGCTG CATCAGTCCC AGGCCCCTCC CAGAGCCTTG GTCTCAATGT CCACAACACG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGTGAA 
GGCGTCAGTT CTCTTAGCAG CTCACCTTCC AACAGCCTTG AGACGCAATC TCAGTCCCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGT GTCTCCTGTG AGAAGAGCAT GTCCCAGGTG 
GATGTGGATT CTGGAATCGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAAGTGT CTGAAGAGCA GGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTGCTG ATGATGTCCA CTCAGACACG AGATGAAAAT 
CCGTTTGCCA GTCTGACGGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
AGAAACCTCA TGCTGAACAC AGGCTCCAGT TCGGGGACAA GTCCCATGTT CTGCAGCTTG 
GGTTCCTTCA GCGCCAGCTC ACTCTCCAGT TTGGGAGCCT CTGGTGGAGC AAGTACCTGG 
GATTCTTACA GTGACCATTT CACCATTGAA ACCTGCAAAG AGACGGACAT GCTGAACTAC 
CTCATCGAGT GCTTTGACCG AGTTGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC 
CAGCCAGCAG TCAGCCAGCT TCTGAGCAAC ATCCGCTCGC AGTGCATCTC CCACACCGCG 
CTAGTGCTAC AAGGGTCCCT CACACAGCCC AGGTCCTTGC AACAGCCATC CTTCCTCGTG 
CCGTACATGC TCTGCAGGAA TCTCCCGTAC GGCTTCATTC AAGAGCTGGT GAGGACCACT 
CACCAGGATG AAGAGGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCGCGT 
GCTGCCAAAG AGTGCTCCTT GGATAGCGAC TACTTTAAGT ACCCCCTCAT GGCACTGGGT 
GAACTCTGTG AAACCAAGTT CGGGAAGACA CACCCTATGT GCAATTTGGT CGCCTCCTTA 
CCCCTGTGGT TGCCGAAGTC CTTAAGCCCT GGTTCTGGGC GGGAGCTGCA GAGACTCTCC 
TACTTAGGGG CCTTCTTTAG CTTCTCTGTC TTCGCAGAGG ACGATGCAAA GGTGGTTGAG 
AAATACTTCT CAGGGCCTGC CATTACCCTG GAGAACACCC GTGTGGTCAG CCAGTCACTA 
CAGCATTACC TGGAGCTGGG ACGGCAAGAG CTTTTCAAGA TTCTGCATAG TATTTTGTTA 
AACGGCGAAA CTCGTGAAGC AGCACTCAGT TACATGGCAG CCATTGTCAA TGCCAATATG 
AAGAAAGCGC AGATGCAGGC AGATGATCGG TTGGTGTCTA CAGACGGGTT CATGCTGAAT 
CTCCTTTGGG TCCTGCAGCA GCTAAGTACA AAAATCAAGT TAGAAACGGT CGACCCCACT 
TACATATTCC ACCCGAGATG TCGGATCACT CTGCCAAACG ATGAGACACG GATAAATGCA 
ACGATGGAGG ATGTGAACGA GTGGCTGACA GAGCTCTATG GAGATCAGCC TCCATTTTCT 
GAGCCAAAAT TCCCTACAGA ATGCTTCTTT CTCACCCTGC ACGCTCACCA TCTCTCTATC 
CTGCCTAGTT GTCGTCGCTA CATTCGCCGG CTCCGAGCCA TCCGTGAGCT CAATAGAACC 
GTGGAAGATT TGAAAAATAA TGAAAGCCAG TGGAAAGATT CCCCACTGGC CACCAGACAC 
CGCGAAATGC TGAAGCGCTG TAAAACTCAG CTTAAGAAAC TGGTACGGTG CAAGGCCTGT 
GCTGACGCTG GCTTACTTGA CGAGAGCTTC CTAAGAAGAT GTCTGAATTT TTATGGCCTT 
CTCATTCAGC TGATGCTGCG CGTCCTGGAC CCTGCATATC CTGACATAAC ACTGCCTTTA 
AATTCAGAAG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TTTATGAGCC CTGCACTCAG 
GACATTGTGA TGTTCCTCGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAACCCTTAC 
CTAGTGGCCA AGCTCGTGGA GGTCATGTTT ATGACCAACC CCTCTGTTCA GCCGAGAACT 
CAGAAGTTTT TTGAGATGAT TGAGAACCAT CCTCTCTCTA CCAAATTGCT GGTACCGTCC 
CTCATGAAGT TCTATACAGA CGTCGAGCAT ACTGGAGCCA CCAGCGAGTT CTATGACAAG 
TTCACAATTC GCTATCACAT CAGCACTATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC 
CATGGCACCT TCATGGAGGA GTTCAATTCT GGAAAGCAGT TCGTTCGGTA TATCAACATG 
CTGATAAATG ACACGACCTT TTTACTGGAC GAAAGTCTGG AGTCTCTGAA GCGGATCCAT 
GAAGTGCAAG AGGAGATGAA GAACCAAGAG CAGTGGGACC AGCTGCCCCG GGATCAGCAG 
CAGGCCCGGC AGTCTCAGCT TGCTCAGGAC GAGCGTGTTT CCCGTTCCTA TCTCGCCCTG 
GCGACTGAAA CCGTGGACAT GTTCCATCTC CTCACCAAGC AAGTCCAGAA GCCGTTCCTG 
AGGCCAGAAC TTGGTCCCCG GTTAGCAGCC ATGCTGAACT TTAACCTGCA GCAGCTCTGC 
GGGCCCAAGT GCCGGGACCT CAAAGTGGAA AACCCAGAGA AGTACGGTTT TGAGCCAAAG 
AAGCTGTTGG ACCAACTGAC GGATATCTAC TTACAGCTGG ACTGTGCTCG CTTTGCTAAA 
GCCATCGCTG ATGACCAGCG ATCCTACAGC AAGGAACTGT TCGAAGAAGT CATTTCAAAG 
ATGCGGAAGG CAGGAATCAA ATCCACCATC GCAATAGAAA AGTTTAAGCT TCTTGCAGAG 
AAAGTGGAGG AAATCGTGGC AAAGAATGCT CGGGCGGAAA TAGACTACAG TGACGCCCCG 
GACGAGTTCA GAGACCCTCT GATGGACACC CTGATGACTG ATCCCGTGAG ACTGCCCTCT 
GGGACCATCA TGGACCGTTC TATCATCCTG CGGCATCTGC TCAACTCCCC CACCGACCCC 
TTCAACCGCC AGATGCTGAC TGAGAGCATG CTGGAGCCAG TGCCAGAGCT AAAGGAGCAG 
ATTCAGGCCT GGATGAGAGA GAAGCAGAGC AGTGACCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube4b ( XM_006239387.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239449.1 Rattus norvegicus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28895
Clone ID Related Accession (Same CDS sequence)	XM_006239387.3
Accession Version	XM_006239387.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239387.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCAGGACCT 
CCAATAGCTG CATCAGTCCC AGGCCCCTCC CAGAGCCTTG GTCTCAATGT CCACAACACG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGTGAA 
GGCGTCAGTT CTCTTAGCAG CTCACCTTCC AACAGCCTTG AGACGCAATC TCAGTCCCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGT GTCTCCTGTG AGAAGAGCAT GTCCCAGGTG 
GATGTGGATT CTGGAATCGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAAGTGT CTGAAGAGCA GGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTGCTG ATGATGTCCA CTCAGACACG AGATGAAAAT 
CCGTTTGCCA GTCTGACGGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
AGAAACCTCA TGCTGAACAC AGGCTCCAGT TCGGGGACAA GTCCCATGTT CTGCAGCTTG 
GGTTCCTTCA GCGCCAGCTC ACTCTCCAGC CTCTATGAAA CGAGCCCCGC CCCCTCTACC 
AGTCTGTGGA GCTTTGTGCC GGGGCTCAGC CCGTCCTTTG CCTCACCTTC CCACACAGCC 
CCCGCCGCCA GCCCTCACAG TTCAGCTCCT CAGGGGGCTG TGGCTTCCCA GTCCCTCAGC 
CCTCAAAGTG CAGCCACGCA TCCCCTGAGT CCCCACAGTG TCGCCCCACA GGCGGCTGTG 
CCTTCCCCGT CCCTCAGCTC CCACAGCACA GCTTCTGTCA TTGCTACCGG AAGCCAGCCG 
TCATCCCCGA GGTATCGGCC CTACACCGTC ACGCACCTGT GGGGGTCTTC ACCTTGCCCC 
ACTCCACGAT CCTCCATTCT TGCTAACCCC CCAGGCTTCC CTGCCCACTC AAGTAGTCCC 
CGGGCAGTAC CTGCCAGCTC CTCCAGACAG AGGCCCCGCA GCAGGGCCCC CGCCTTCCCA 
CCCGCCTCGC CCAGTGCTGC CAGCAGACGT CCCTCCTCCC TGCGGATCTC TCCTAGTATG 
TACGACAGTC CTTTCTCCTC CCTCTTCCTC GCACTTTCTG GGGACAGTAG TGAAGAAGAA 
GAAGAAGAAG ATGAGGAGGA TGATGATGAA GGTGGTGGTG ATGATGATTT TTCTTGTGTC 
CAGTTTGGGT CCAGTTTGGG AGCCTCTGGT GGAGCAAGTA CCTGGGATTC TTACAGTGAC 
CATTTCACCA TTGAAACCTG CAAAGAGACG GACATGCTGA ACTACCTCAT CGAGTGCTTT 
GACCGAGTTG GAATAGAGGA AAAAAAAGCA CCAAAGATGT GCAGCCAGCC AGCAGTCAGC 
CAGCTTCTGA GCAACATCCG CTCGCAGTGC ATCTCCCACA CCGCGCTAGT GCTACAAGGG 
TCCCTCACAC AGCCCAGGTC CTTGCAACAG CCATCCTTCC TCGTGCCGTA CATGCTCTGC 
AGGAATCTCC CGTACGGCTT CATTCAAGAG CTGGTGAGGA CCACTCACCA GGATGAAGAG 
GTGTTCAAGC AGATCTTTAT CCCCATTTTA CAAGGCCTGG CGCGTGCTGC CAAAGAGTGC 
TCCTTGGATA GCGACTACTT TAAGTACCCC CTCATGGCAC TGGGTGAACT CTGTGAAACC 
AAGTTCGGGA AGACACACCC TATGTGCAAT TTGGTCGCCT CCTTACCCCT GTGGTTGCCG 
AAGTCCTTAA GCCCTGGTTC TGGGCGGGAG CTGCAGAGAC TCTCCTACTT AGGGGCCTTC 
TTTAGCTTCT CTGTCTTCGC AGAGGACGAT GCAAAGGTGG TTGAGAAATA CTTCTCAGGG 
CCTGCCATTA CCCTGGAGAA CACCCGTGTG GTCAGCCAGT CACTACAGCA TTACCTGGAG 
CTGGGACGGC AAGAGCTTTT CAAGATTCTG CATAGTATTT TGTTAAACGG CGAAACTCGT 
GAAGCAGCAC TCAGTTACAT GGCAGCCATT GTCAATGCCA ATATGAAGAA AGCGCAGATG 
CAGGCAGATG ATCGGTTGGT GTCTACAGAC GGGTTCATGC TGAATCTCCT TTGGGTCCTG 
CAGCAGCTAA GTACAAAAAT CAAGTTAGAA ACGGTCGACC CCACTTACAT ATTCCACCCG 
AGATGTCGGA TCACTCTGCC AAACGATGAG ACACGGATAA ATGCAACGAT GGAGGATGTG 
AACGAGTGGC TGACAGAGCT CTATGGAGAT CAGCCTCCAT TTTCTGAGCC AAAATTCCCT 
ACAGAATGCT TCTTTCTCAC CCTGCACGCT CACCATCTCT CTATCCTGCC TAGTTGTCGT 
CGCTACATTC GCCGGCTCCG AGCCATCCGT GAGCTCAATA GAACCGTGGA AGATTTGAAA 
AATAATGAAA GCCAGTGGAA AGATTCCCCA CTGGCCACCA GACACCGCGA AATGCTGAAG 
CGCTGTAAAA CTCAGCTTAA GAAACTGGTA CGGTGCAAGG CCTGTGCTGA CGCTGGCTTA 
CTTGACGAGA GCTTCCTAAG AAGATGTCTG AATTTTTATG GCCTTCTCAT TCAGCTGATG 
CTGCGCGTCC TGGACCCTGC ATATCCTGAC ATAACACTGC CTTTAAATTC AGAAGTCCCC 
AAGGTATTTG CAGCATTGCC TGAGTTTTAT GTAGAAGATG TTGCTGAATT TTTATTTTTT 
ATTGTACAAT ACTCTCCTCA GGTGCTTTAT GAGCCCTGCA CTCAGGACAT TGTGATGTTC 
CTCGTTGTGA TGCTGTGCAA CCAGAACTAC ATCCGAAACC CTTACCTAGT GGCCAAGCTC 
GTGGAGGTCA TGTTTATGAC CAACCCCTCT GTTCAGCCGA GAACTCAGAA GTTTTTTGAG 
ATGATTGAGA ACCATCCTCT CTCTACCAAA TTGCTGGTAC CGTCCCTCAT GAAGTTCTAT 
ACAGACGTCG AGCATACTGG AGCCACCAGC GAGTTCTATG ACAAGTTCAC AATTCGCTAT 
CACATCAGCA CTATTTTTAA AAGCCTTTGG CAAAACATAG CTCACCATGG CACCTTCATG 
GAGGAGTTCA ATTCTGGAAA GCAGTTCGTT CGGTATATCA ACATGCTGAT AAATGACACG 
ACCTTTTTAC TGGACGAAAG TCTGGAGTCT CTGAAGCGGA TCCATGAAGT GCAAGAGGAG 
ATGAAGAACC AAGAGCAGTG GGACCAGCTG CCCCGGGATC AGCAGCAGGC CCGGCAGTCT 
CAGCTTGCTC AGGACGAGCG TGTTTCCCGT TCCTATCTCG CCCTGGCGAC TGAAACCGTG 
GACATGTTCC ATCTCCTCAC CAAGCAAGTC CAGAAGCCGT TCCTGAGGCC AGAACTTGGT 
CCCCGGTTAG CAGCCATGCT GAACTTTAAC CTGCAGCAGC TCTGCGGGCC CAAGTGCCGG 
GACCTCAAAG TGGAAAACCC AGAGAAGTAC GGTTTTGAGC CAAAGAAGCT GTTGGACCAA 
CTGACGGATA TCTACTTACA GCTGGACTGT GCTCGCTTTG CTAAAGCCAT CGCTGATGAC 
CAGCGATCCT ACAGCAAGGA ACTGTTCGAA GAAGTCATTT CAAAGATGCG GAAGGCAGGA 
ATCAAATCCA CCATCGCAAT AGAAAAGTTT AAGCTTCTTG CAGAGAAAGT GGAGGAAATC 
GTGGCAAAGA ATGCTCGGGC GGAAATAGAC TACAGTGACG CCCCGGACGA GTTCAGAGAC 
CCTCTGATGG ACACCCTGAT GACTGATCCC GTGAGACTGC CCTCTGGGAC CATCATGGAC 
CGTTCTATCA TCCTGCGGCA TCTGCTCAAC TCCCCCACCG ACCCCTTCAA CCGCCAGATG 
CTGACTGAGA GCATGCTGGA GCCAGTGCCA GAGCTAAAGG AGCAGATTCA GGCCTGGATG 
AGAGAGAAGC AGAGCAGTGA CCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239449.1
CDS	729..4895
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASVPGPSQSLGLNVHNTTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCSLGSFSASSLSSLYETSPAPSTSLWSFVPGLSPSFASPSHTAPAASPHSSAPQGAVASQSLSPQSAATHPLSPHSVAPQAAVPSPSLSSHSTASVIATGSQPSSPRYRPYTVTHLWGSSPCPTPRSSILANPPGFPAHSSSPRAVPASSSRQRPRSRAPAFPPASPSAASRRPSSLRISPSMYDSPFSSLFLALSGDSSEEEEEEDEEDDDEGGGDDDFSCVQFGSSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLARAAKECSLDSDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAIVNANMKKAQMQADDRLVSTDGFMLNLLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNEWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRVLDPAYPDITLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNQEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH

Target ORF information:

RefSeq Version	XM_006239387.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239387.3

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCAGGACCT 
CCAATAGCTG CATCAGTCCC AGGCCCCTCC CAGAGCCTTG GTCTCAATGT CCACAACACG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGTGAA 
GGCGTCAGTT CTCTTAGCAG CTCACCTTCC AACAGCCTTG AGACGCAATC TCAGTCCCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGT GTCTCCTGTG AGAAGAGCAT GTCCCAGGTG 
GATGTGGATT CTGGAATCGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAAGTGT CTGAAGAGCA GGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTGCTG ATGATGTCCA CTCAGACACG AGATGAAAAT 
CCGTTTGCCA GTCTGACGGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
AGAAACCTCA TGCTGAACAC AGGCTCCAGT TCGGGGACAA GTCCCATGTT CTGCAGCTTG 
GGTTCCTTCA GCGCCAGCTC ACTCTCCAGC CTCTATGAAA CGAGCCCCGC CCCCTCTACC 
AGTCTGTGGA GCTTTGTGCC GGGGCTCAGC CCGTCCTTTG CCTCACCTTC CCACACAGCC 
CCCGCCGCCA GCCCTCACAG TTCAGCTCCT CAGGGGGCTG TGGCTTCCCA GTCCCTCAGC 
CCTCAAAGTG CAGCCACGCA TCCCCTGAGT CCCCACAGTG TCGCCCCACA GGCGGCTGTG 
CCTTCCCCGT CCCTCAGCTC CCACAGCACA GCTTCTGTCA TTGCTACCGG AAGCCAGCCG 
TCATCCCCGA GGTATCGGCC CTACACCGTC ACGCACCTGT GGGGGTCTTC ACCTTGCCCC 
ACTCCACGAT CCTCCATTCT TGCTAACCCC CCAGGCTTCC CTGCCCACTC AAGTAGTCCC 
CGGGCAGTAC CTGCCAGCTC CTCCAGACAG AGGCCCCGCA GCAGGGCCCC CGCCTTCCCA 
CCCGCCTCGC CCAGTGCTGC CAGCAGACGT CCCTCCTCCC TGCGGATCTC TCCTAGTATG 
TACGACAGTC CTTTCTCCTC CCTCTTCCTC GCACTTTCTG GGGACAGTAG TGAAGAAGAA 
GAAGAAGAAG ATGAGGAGGA TGATGATGAA GGTGGTGGTG ATGATGATTT TTCTTGTGTC 
CAGTTTGGGT CCAGTTTGGG AGCCTCTGGT GGAGCAAGTA CCTGGGATTC TTACAGTGAC 
CATTTCACCA TTGAAACCTG CAAAGAGACG GACATGCTGA ACTACCTCAT CGAGTGCTTT 
GACCGAGTTG GAATAGAGGA AAAAAAAGCA CCAAAGATGT GCAGCCAGCC AGCAGTCAGC 
CAGCTTCTGA GCAACATCCG CTCGCAGTGC ATCTCCCACA CCGCGCTAGT GCTACAAGGG 
TCCCTCACAC AGCCCAGGTC CTTGCAACAG CCATCCTTCC TCGTGCCGTA CATGCTCTGC 
AGGAATCTCC CGTACGGCTT CATTCAAGAG CTGGTGAGGA CCACTCACCA GGATGAAGAG 
GTGTTCAAGC AGATCTTTAT CCCCATTTTA CAAGGCCTGG CGCGTGCTGC CAAAGAGTGC 
TCCTTGGATA GCGACTACTT TAAGTACCCC CTCATGGCAC TGGGTGAACT CTGTGAAACC 
AAGTTCGGGA AGACACACCC TATGTGCAAT TTGGTCGCCT CCTTACCCCT GTGGTTGCCG 
AAGTCCTTAA GCCCTGGTTC TGGGCGGGAG CTGCAGAGAC TCTCCTACTT AGGGGCCTTC 
TTTAGCTTCT CTGTCTTCGC AGAGGACGAT GCAAAGGTGG TTGAGAAATA CTTCTCAGGG 
CCTGCCATTA CCCTGGAGAA CACCCGTGTG GTCAGCCAGT CACTACAGCA TTACCTGGAG 
CTGGGACGGC AAGAGCTTTT CAAGATTCTG CATAGTATTT TGTTAAACGG CGAAACTCGT 
GAAGCAGCAC TCAGTTACAT GGCAGCCATT GTCAATGCCA ATATGAAGAA AGCGCAGATG 
CAGGCAGATG ATCGGTTGGT GTCTACAGAC GGGTTCATGC TGAATCTCCT TTGGGTCCTG 
CAGCAGCTAA GTACAAAAAT CAAGTTAGAA ACGGTCGACC CCACTTACAT ATTCCACCCG 
AGATGTCGGA TCACTCTGCC AAACGATGAG ACACGGATAA ATGCAACGAT GGAGGATGTG 
AACGAGTGGC TGACAGAGCT CTATGGAGAT CAGCCTCCAT TTTCTGAGCC AAAATTCCCT 
ACAGAATGCT TCTTTCTCAC CCTGCACGCT CACCATCTCT CTATCCTGCC TAGTTGTCGT 
CGCTACATTC GCCGGCTCCG AGCCATCCGT GAGCTCAATA GAACCGTGGA AGATTTGAAA 
AATAATGAAA GCCAGTGGAA AGATTCCCCA CTGGCCACCA GACACCGCGA AATGCTGAAG 
CGCTGTAAAA CTCAGCTTAA GAAACTGGTA CGGTGCAAGG CCTGTGCTGA CGCTGGCTTA 
CTTGACGAGA GCTTCCTAAG AAGATGTCTG AATTTTTATG GCCTTCTCAT TCAGCTGATG 
CTGCGCGTCC TGGACCCTGC ATATCCTGAC ATAACACTGC CTTTAAATTC AGAAGTCCCC 
AAGGTATTTG CAGCATTGCC TGAGTTTTAT GTAGAAGATG TTGCTGAATT TTTATTTTTT 
ATTGTACAAT ACTCTCCTCA GGTGCTTTAT GAGCCCTGCA CTCAGGACAT TGTGATGTTC 
CTCGTTGTGA TGCTGTGCAA CCAGAACTAC ATCCGAAACC CTTACCTAGT GGCCAAGCTC 
GTGGAGGTCA TGTTTATGAC CAACCCCTCT GTTCAGCCGA GAACTCAGAA GTTTTTTGAG 
ATGATTGAGA ACCATCCTCT CTCTACCAAA TTGCTGGTAC CGTCCCTCAT GAAGTTCTAT 
ACAGACGTCG AGCATACTGG AGCCACCAGC GAGTTCTATG ACAAGTTCAC AATTCGCTAT 
CACATCAGCA CTATTTTTAA AAGCCTTTGG CAAAACATAG CTCACCATGG CACCTTCATG 
GAGGAGTTCA ATTCTGGAAA GCAGTTCGTT CGGTATATCA ACATGCTGAT AAATGACACG 
ACCTTTTTAC TGGACGAAAG TCTGGAGTCT CTGAAGCGGA TCCATGAAGT GCAAGAGGAG 
ATGAAGAACC AAGAGCAGTG GGACCAGCTG CCCCGGGATC AGCAGCAGGC CCGGCAGTCT 
CAGCTTGCTC AGGACGAGCG TGTTTCCCGT TCCTATCTCG CCCTGGCGAC TGAAACCGTG 
GACATGTTCC ATCTCCTCAC CAAGCAAGTC CAGAAGCCGT TCCTGAGGCC AGAACTTGGT 
CCCCGGTTAG CAGCCATGCT GAACTTTAAC CTGCAGCAGC TCTGCGGGCC CAAGTGCCGG 
GACCTCAAAG TGGAAAACCC AGAGAAGTAC GGTTTTGAGC CAAAGAAGCT GTTGGACCAA 
CTGACGGATA TCTACTTACA GCTGGACTGT GCTCGCTTTG CTAAAGCCAT CGCTGATGAC 
CAGCGATCCT ACAGCAAGGA ACTGTTCGAA GAAGTCATTT CAAAGATGCG GAAGGCAGGA 
ATCAAATCCA CCATCGCAAT AGAAAAGTTT AAGCTTCTTG CAGAGAAAGT GGAGGAAATC 
GTGGCAAAGA ATGCTCGGGC GGAAATAGAC TACAGTGACG CCCCGGACGA GTTCAGAGAC 
CCTCTGATGG ACACCCTGAT GACTGATCCC GTGAGACTGC CCTCTGGGAC CATCATGGAC 
CGTTCTATCA TCCTGCGGCA TCTGCTCAAC TCCCCCACCG ACCCCTTCAA CCGCCAGATG 
CTGACTGAGA GCATGCTGGA GCCAGTGCCA GAGCTAAAGG AGCAGATTCA GGCCTGGATG 
AGAGAGAAGC AGAGCAGTGA CCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube4b ( XM_017593310.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448799.1 Rattus norvegicus ubiquitination factor E4B (Ube4b), transcript variant X2, mRNA.

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CloneID	ORa61189
Clone ID Related Accession (Same CDS sequence)	XM_017593310.1
Accession Version	XM_017593310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin conjugation factor E4 B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCAGGACCT 
CCAATAGCTG CATCAGTCCC AGGCCCCTCC CAGAGCCTTG GTCTCAATGT CCACAACACG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGTGAA 
GGCGTCAGTT CTCTTAGCAG CTCACCTTCC AACAGCCTTG AGACGCAATC TCAGTCCCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGT GTCTCCTGTG AGAAGAGCAT GTCCCAGGTG 
GATGTGGATT CTGGAATCGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAAGTGT CTGAAGAGCA GGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTGCTG ATGATGTCCA CTCAGACACG AGATGAAAAT 
CCGTTTGCCA GTCTGACGGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
AGAAACCTCA TGCTGAACAC AGGCTCCAGT TCGGGGACAA GTCCCATGTT CTGCAGCTTG 
GGTTCCTTCA GCGCCAGCTC ACTCTCCAGC CTCTATGAAA CGAGCCCCGC CCCCTCTACC 
AGTCTGTGGA GCTTTGTGCC GGGGCTCAGC CCGTCCTTTG CCTCACCTTC CCACACAGCC 
CCCGCCGCCA GCCCTCACAG TTCAGCTCCT CAGGGGGCTG TGGCTTCCCA GTCCCTCAGC 
CCTCAAAGTG CAGCCACGCA TCCCCTGAGT CCCCACAGTG TCGCCCCACA GGCGGCTGTG 
CCTTCCCCGT CCCTCAGCTC CCACAGCACA GCTTCTGTCA TTGCTACCGG AAGCCAGCCG 
TCATCCCCGA GGTATCGGCC CTACACCGTC ACGCACCTGT GGGGGTCTTC ACCTTGCCCC 
ACTCCACGAT CCTCCATTCT TGCTAACCCC CCAGGCTTCC CTGCCCACTC AAGTAGTCCC 
CGGGCAGTAC CTGCCAGCTC CTCCAGACAG AGGCCCCGCA GCAGGGCCCC CGCCTTCCCA 
CCCGCCTCGC CCAGTGCTGC CAGCAGACGT CCCTCCTCCC TGCGGATCTC TCCTAGTTTG 
GGAGCCTCTG GTGGAGCAAG TACCTGGGAT TCTTACAGTG ACCATTTCAC CATTGAAACC 
TGCAAAGAGA CGGACATGCT GAACTACCTC ATCGAGTGCT TTGACCGAGT TGGAATAGAG 
GAAAAAAAAG CACCAAAGAT GTGCAGCCAG CCAGCAGTCA GCCAGCTTCT GAGCAACATC 
CGCTCGCAGT GCATCTCCCA CACCGCGCTA GTGCTACAAG GGTCCCTCAC ACAGCCCAGG 
TCCTTGCAAC AGCCATCCTT CCTCGTGCCG TACATGCTCT GCAGGAATCT CCCGTACGGC 
TTCATTCAAG AGCTGGTGAG GACCACTCAC CAGGATGAAG AGGTGTTCAA GCAGATCTTT 
ATCCCCATTT TACAAGGCCT GGCGCGTGCT GCCAAAGAGT GCTCCTTGGA TAGCGACTAC 
TTTAAGTACC CCCTCATGGC ACTGGGTGAA CTCTGTGAAA CCAAGTTCGG GAAGACACAC 
CCTATGTGCA ATTTGGTCGC CTCCTTACCC CTGTGGTTGC CGAAGTCCTT AAGCCCTGGT 
TCTGGGCGGG AGCTGCAGAG ACTCTCCTAC TTAGGGGCCT TCTTTAGCTT CTCTGTCTTC 
GCAGAGGACG ATGCAAAGGT GGTTGAGAAA TACTTCTCAG GGCCTGCCAT TACCCTGGAG 
AACACCCGTG TGGTCAGCCA GTCACTACAG CATTACCTGG AGCTGGGACG GCAAGAGCTT 
TTCAAGATTC TGCATAGTAT TTTGTTAAAC GGCGAAACTC GTGAAGCAGC ACTCAGTTAC 
ATGGCAGCCA TTGTCAATGC CAATATGAAG AAAGCGCAGA TGCAGGCAGA TGATCGGTTG 
GTGTCTACAG ACGGGTTCAT GCTGAATCTC CTTTGGGTCC TGCAGCAGCT AAGTACAAAA 
ATCAAGTTAG AAACGGTCGA CCCCACTTAC ATATTCCACC CGAGATGTCG GATCACTCTG 
CCAAACGATG AGACACGGAT AAATGCAACG ATGGAGGATG TGAACGAGTG GCTGACAGAG 
CTCTATGGAG ATCAGCCTCC ATTTTCTGAG CCAAAATTCC CTACAGAATG CTTCTTTCTC 
ACCCTGCACG CTCACCATCT CTCTATCCTG CCTAGTTGTC GTCGCTACAT TCGCCGGCTC 
CGAGCCATCC GTGAGCTCAA TAGAACCGTG GAAGATTTGA AAAATAATGA AAGCCAGTGG 
AAAGATTCCC CACTGGCCAC CAGACACCGC GAAATGCTGA AGCGCTGTAA AACTCAGCTT 
AAGAAACTGG TACGGTGCAA GGCCTGTGCT GACGCTGGCT TACTTGACGA GAGCTTCCTA 
AGAAGATGTC TGAATTTTTA TGGCCTTCTC ATTCAGCTGA TGCTGCGCGT CCTGGACCCT 
GCATATCCTG ACATAACACT GCCTTTAAAT TCAGAAGTCC CCAAGGTATT TGCAGCATTG 
CCTGAGTTTT ATGTAGAAGA TGTTGCTGAA TTTTTATTTT TTATTGTACA ATACTCTCCT 
CAGGTGCTTT ATGAGCCCTG CACTCAGGAC ATTGTGATGT TCCTCGTTGT GATGCTGTGC 
AACCAGAACT ACATCCGAAA CCCTTACCTA GTGGCCAAGC TCGTGGAGGT CATGTTTATG 
ACCAACCCCT CTGTTCAGCC GAGAACTCAG AAGTTTTTTG AGATGATTGA GAACCATCCT 
CTCTCTACCA AATTGCTGGT ACCGTCCCTC ATGAAGTTCT ATACAGACGT CGAGCATACT 
GGAGCCACCA GCGAGTTCTA TGACAAGTTC ACAATTCGCT ATCACATCAG CACTATTTTT 
AAAAGCCTTT GGCAAAACAT AGCTCACCAT GGCACCTTCA TGGAGGAGTT CAATTCTGGA 
AAGCAGTTCG TTCGGTATAT CAACATGCTG ATAAATGACA CGACCTTTTT ACTGGACGAA 
AGTCTGGAGT CTCTGAAGCG GATCCATGAA GTGCAAGAGG AGATGAAGAA CCAAGAGCAG 
TGGGACCAGC TGCCCCGGGA TCAGCAGCAG GCCCGGCAGT CTCAGCTTGC TCAGGACGAG 
CGTGTTTCCC GTTCCTATCT CGCCCTGGCG ACTGAAACCG TGGACATGTT CCATCTCCTC 
ACCAAGCAAG TCCAGAAGCC GTTCCTGAGG CCAGAACTTG GTCCCCGGTT AGCAGCCATG 
CTGAACTTTA ACCTGCAGCA GCTCTGCGGG CCCAAGTGCC GGGACCTCAA AGTGGAAAAC 
CCAGAGAAGT ACGGTTTTGA GCCAAAGAAG CTGTTGGACC AACTGACGGA TATCTACTTA 
CAGCTGGACT GTGCTCGCTT TGCTAAAGCC ATCGCTGATG ACCAGCGATC CTACAGCAAG 
GAACTGTTCG AAGAAGTCAT TTCAAAGATG CGGAAGGCAG GAATCAAATC CACCATCGCA 
ATAGAAAAGT TTAAGCTTCT TGCAGAGAAA GTGGAGGAAA TCGTGGCAAA GAATGCTCGG 
GCGGAAATAG ACTACAGTGA CGCCCCGGAC GAGTTCAGAG ACCCTCTGAT GGACACCCTG 
ATGACTGATC CCGTGAGACT GCCCTCTGGG ACCATCATGG ACCGTTCTAT CATCCTGCGG 
CATCTGCTCA ACTCCCCCAC CGACCCCTTC AACCGCCAGA TGCTGACTGA GAGCATGCTG 
GAGCCAGTGC CAGAGCTAAA GGAGCAGATT CAGGCCTGGA TGAGAGAGAA GCAGAGCAGT 
GACCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448799.1
CDS	728..4756
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASVPGPSQSLGLNVHNTTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCSLGSFSASSLSSLYETSPAPSTSLWSFVPGLSPSFASPSHTAPAASPHSSAPQGAVASQSLSPQSAATHPLSPHSVAPQAAVPSPSLSSHSTASVIATGSQPSSPRYRPYTVTHLWGSSPCPTPRSSILANPPGFPAHSSSPRAVPASSSRQRPRSRAPAFPPASPSAASRRPSSLRISPSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLARAAKECSLDSDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAIVNANMKKAQMQADDRLVSTDGFMLNLLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNEWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRVLDPAYPDITLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNQEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH

Target ORF information:

RefSeq Version	XM_017593310.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitination factor E4B (Ube4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593310.1

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCAGGACCT 
CCAATAGCTG CATCAGTCCC AGGCCCCTCC CAGAGCCTTG GTCTCAATGT CCACAACACG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGTGAA 
GGCGTCAGTT CTCTTAGCAG CTCACCTTCC AACAGCCTTG AGACGCAATC TCAGTCCCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGT GTCTCCTGTG AGAAGAGCAT GTCCCAGGTG 
GATGTGGATT CTGGAATCGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAAGTGT CTGAAGAGCA GGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTGCTG ATGATGTCCA CTCAGACACG AGATGAAAAT 
CCGTTTGCCA GTCTGACGGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
AGAAACCTCA TGCTGAACAC AGGCTCCAGT TCGGGGACAA GTCCCATGTT CTGCAGCTTG 
GGTTCCTTCA GCGCCAGCTC ACTCTCCAGC CTCTATGAAA CGAGCCCCGC CCCCTCTACC 
AGTCTGTGGA GCTTTGTGCC GGGGCTCAGC CCGTCCTTTG CCTCACCTTC CCACACAGCC 
CCCGCCGCCA GCCCTCACAG TTCAGCTCCT CAGGGGGCTG TGGCTTCCCA GTCCCTCAGC 
CCTCAAAGTG CAGCCACGCA TCCCCTGAGT CCCCACAGTG TCGCCCCACA GGCGGCTGTG 
CCTTCCCCGT CCCTCAGCTC CCACAGCACA GCTTCTGTCA TTGCTACCGG AAGCCAGCCG 
TCATCCCCGA GGTATCGGCC CTACACCGTC ACGCACCTGT GGGGGTCTTC ACCTTGCCCC 
ACTCCACGAT CCTCCATTCT TGCTAACCCC CCAGGCTTCC CTGCCCACTC AAGTAGTCCC 
CGGGCAGTAC CTGCCAGCTC CTCCAGACAG AGGCCCCGCA GCAGGGCCCC CGCCTTCCCA 
CCCGCCTCGC CCAGTGCTGC CAGCAGACGT CCCTCCTCCC TGCGGATCTC TCCTAGTTTG 
GGAGCCTCTG GTGGAGCAAG TACCTGGGAT TCTTACAGTG ACCATTTCAC CATTGAAACC 
TGCAAAGAGA CGGACATGCT GAACTACCTC ATCGAGTGCT TTGACCGAGT TGGAATAGAG 
GAAAAAAAAG CACCAAAGAT GTGCAGCCAG CCAGCAGTCA GCCAGCTTCT GAGCAACATC 
CGCTCGCAGT GCATCTCCCA CACCGCGCTA GTGCTACAAG GGTCCCTCAC ACAGCCCAGG 
TCCTTGCAAC AGCCATCCTT CCTCGTGCCG TACATGCTCT GCAGGAATCT CCCGTACGGC 
TTCATTCAAG AGCTGGTGAG GACCACTCAC CAGGATGAAG AGGTGTTCAA GCAGATCTTT 
ATCCCCATTT TACAAGGCCT GGCGCGTGCT GCCAAAGAGT GCTCCTTGGA TAGCGACTAC 
TTTAAGTACC CCCTCATGGC ACTGGGTGAA CTCTGTGAAA CCAAGTTCGG GAAGACACAC 
CCTATGTGCA ATTTGGTCGC CTCCTTACCC CTGTGGTTGC CGAAGTCCTT AAGCCCTGGT 
TCTGGGCGGG AGCTGCAGAG ACTCTCCTAC TTAGGGGCCT TCTTTAGCTT CTCTGTCTTC 
GCAGAGGACG ATGCAAAGGT GGTTGAGAAA TACTTCTCAG GGCCTGCCAT TACCCTGGAG 
AACACCCGTG TGGTCAGCCA GTCACTACAG CATTACCTGG AGCTGGGACG GCAAGAGCTT 
TTCAAGATTC TGCATAGTAT TTTGTTAAAC GGCGAAACTC GTGAAGCAGC ACTCAGTTAC 
ATGGCAGCCA TTGTCAATGC CAATATGAAG AAAGCGCAGA TGCAGGCAGA TGATCGGTTG 
GTGTCTACAG ACGGGTTCAT GCTGAATCTC CTTTGGGTCC TGCAGCAGCT AAGTACAAAA 
ATCAAGTTAG AAACGGTCGA CCCCACTTAC ATATTCCACC CGAGATGTCG GATCACTCTG 
CCAAACGATG AGACACGGAT AAATGCAACG ATGGAGGATG TGAACGAGTG GCTGACAGAG 
CTCTATGGAG ATCAGCCTCC ATTTTCTGAG CCAAAATTCC CTACAGAATG CTTCTTTCTC 
ACCCTGCACG CTCACCATCT CTCTATCCTG CCTAGTTGTC GTCGCTACAT TCGCCGGCTC 
CGAGCCATCC GTGAGCTCAA TAGAACCGTG GAAGATTTGA AAAATAATGA AAGCCAGTGG 
AAAGATTCCC CACTGGCCAC CAGACACCGC GAAATGCTGA AGCGCTGTAA AACTCAGCTT 
AAGAAACTGG TACGGTGCAA GGCCTGTGCT GACGCTGGCT TACTTGACGA GAGCTTCCTA 
AGAAGATGTC TGAATTTTTA TGGCCTTCTC ATTCAGCTGA TGCTGCGCGT CCTGGACCCT 
GCATATCCTG ACATAACACT GCCTTTAAAT TCAGAAGTCC CCAAGGTATT TGCAGCATTG 
CCTGAGTTTT ATGTAGAAGA TGTTGCTGAA TTTTTATTTT TTATTGTACA ATACTCTCCT 
CAGGTGCTTT ATGAGCCCTG CACTCAGGAC ATTGTGATGT TCCTCGTTGT GATGCTGTGC 
AACCAGAACT ACATCCGAAA CCCTTACCTA GTGGCCAAGC TCGTGGAGGT CATGTTTATG 
ACCAACCCCT CTGTTCAGCC GAGAACTCAG AAGTTTTTTG AGATGATTGA GAACCATCCT 
CTCTCTACCA AATTGCTGGT ACCGTCCCTC ATGAAGTTCT ATACAGACGT CGAGCATACT 
GGAGCCACCA GCGAGTTCTA TGACAAGTTC ACAATTCGCT ATCACATCAG CACTATTTTT 
AAAAGCCTTT GGCAAAACAT AGCTCACCAT GGCACCTTCA TGGAGGAGTT CAATTCTGGA 
AAGCAGTTCG TTCGGTATAT CAACATGCTG ATAAATGACA CGACCTTTTT ACTGGACGAA 
AGTCTGGAGT CTCTGAAGCG GATCCATGAA GTGCAAGAGG AGATGAAGAA CCAAGAGCAG 
TGGGACCAGC TGCCCCGGGA TCAGCAGCAG GCCCGGCAGT CTCAGCTTGC TCAGGACGAG 
CGTGTTTCCC GTTCCTATCT CGCCCTGGCG ACTGAAACCG TGGACATGTT CCATCTCCTC 
ACCAAGCAAG TCCAGAAGCC GTTCCTGAGG CCAGAACTTG GTCCCCGGTT AGCAGCCATG 
CTGAACTTTA ACCTGCAGCA GCTCTGCGGG CCCAAGTGCC GGGACCTCAA AGTGGAAAAC 
CCAGAGAAGT ACGGTTTTGA GCCAAAGAAG CTGTTGGACC AACTGACGGA TATCTACTTA 
CAGCTGGACT GTGCTCGCTT TGCTAAAGCC ATCGCTGATG ACCAGCGATC CTACAGCAAG 
GAACTGTTCG AAGAAGTCAT TTCAAAGATG CGGAAGGCAG GAATCAAATC CACCATCGCA 
ATAGAAAAGT TTAAGCTTCT TGCAGAGAAA GTGGAGGAAA TCGTGGCAAA GAATGCTCGG 
GCGGAAATAG ACTACAGTGA CGCCCCGGAC GAGTTCAGAG ACCCTCTGAT GGACACCCTG 
ATGACTGATC CCGTGAGACT GCCCTCTGGG ACCATCATGG ACCGTTCTAT CATCCTGCGG 
CATCTGCTCA ACTCCCCCAC CGACCCCTTC AACCGCCAGA TGCTGACTGA GAGCATGCTG 
GAGCCAGTGC CAGAGCTAAA GGAGCAGATT CAGGCCTGGA TGAGAGAGAA GCAGAGCAGT 
GACCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis.
Proceedings of the National Academy of Sciences of the United States of America110(14)E1263-72(2013 Apr)
Starita LM,Pruneda JN,Lo RS,Fowler DM,Kim HJ,Hiatt JB,Shendure J,Brzovic PS,Fields S,Klevit RE

Structural and functional characterization of the monomeric U-box domain from E4B.
Biochemistry49(2)347-55(2010 Jan)
Nordquist KA,Dimitrova YN,Brzovic PS,Ridenour WB,Munro KA,Soss SE,Caprioli RM,Klevit RE,Chazin WJ

The WldS gene delays axonal but not somatic degeneration in a rat glaucoma model.
The European journal of neuroscience28(6)1166-79(2008 Sep)
Beirowski B,Babetto E,Coleman MP,Martin KR

Mammalian E4 is required for cardiac development and maintenance of the nervous system.
Molecular and cellular biology25(24)10953-64(2005 Dec)
Kaneko-Oshikawa C,Nakagawa T,Yamada M,Yoshikawa H,Matsumoto M,Yada M,Hatakeyama S,Nakayama K,Nakayama KI

Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis.
The Journal of biological chemistry279(51)53533-43(2004 Dec)
Okumura F,Hatakeyama S,Matsumoto M,Kamura T,Nakayama KI

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Ube4b cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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